COMPOSITION FOR PROMOTING INCREASE IN SKIN VOLUME

The present invention refers to case ratio , volume hundred , astuteness , radix salviae miltiorrhizae, mint, against, wheat, blending, paper and selected from the group consisting of phosphorous in the pieces 1 to at least one fat cell stimulating growth composition.

In a term "the adipocyte differentiation" in the present invention using the animal, preferably mammalian fatty precursor cells or intermediate the lobe, the stem cell fat of differentiating into which is used, intermediate preferably human than the lobe, the stem cell , most preferably human bone marrow derived from intermediate the lobe, the stem cell by a rope. of differentiating into fat.

Using in the present invention has excellent inhibition effect on to equal.

Specific case ratio ([...]) (Castaneae Semen) and little under. enclose an properties which the moon. Diarrhea strong stomach lower and is the health [...] ability receiver which cough, leg military force symptoms. use in. Hemostatic action and in addition is connected to the semiconductor layer. such bronchitis. Pharmacological effect been the report antioxidation activity at rich in vitamin C skin care, fatigue recovery, the like preventing wound report described.

volume hundred ([...]) (Selaginella tamariscina) are odour-less and which of the properties which the map. the thermocouple without and spring biased. Various lower bleeding faecal, so that it is useful stanched bleeding, hematemesis , [...] , which dissolving the solid derivative into uterine bleeding, blood row food in potent recycle menstrual colic or when there is no menses, bruise is used or the like. Staphylococcal yellow at pharmacological effect possess inhibitory activity has been reported.

astuteness (l. Aloe vera) has aloesin, speed (Aloe) a plant belonging to the entire indicating or one of mean. Africa and chickens and is, about 300 paper around the world. tube lobe into plants grown in greenhouse at home in a dome-like manner in medicine or shelve in a. also. To mold bacteria aerator has a and toxin that antagonize which and a source of both [...] aloe, aloe effect are ulcer having anticancer activity [...][...] example a the registrar. Even out, steroid, amino acid, saponin, antibiotic, wound healing hormone, determining the dimensions of the coldbox pertaining inorganic of a polyimide resin, such. and a source of both. Aloe a from being deformed due to hangover due to repeats one or and refreshment is as or the like, a face, improved brightness to leaf aloe exceptionally bitter yellow material runs which, . in particular rapidly swells in this. Or ingesting to upset stomach fractioncopyright 2000 leaf aloe in civilian and trauma or Image-proof packing use. In addition the neutralizing oily skin and dry skin cosmetic raw material having skin moisturizing effect there is a cosmetic raw material, even with. written.

Mint ([...]) (var Mentha arvensis . Piperascens Makinv.) the health if placing in a mouth is peripheral surface with different tempos kick and map. The efficiency external sensitivity closed because of the a heating, headache, SSM it holds decongestion of the eye in using of eucommia, throat salt , tonsil salt, measles initial ponciri fructus, liver disease, visceral pain, diarrhea, vomiting is used or the like. Pharmacological action antipyretic, inflammatory, strong stomach , bile secretion promoting and gastrointestinal smooth muscle inhibitors, release of the mucus cleaned, central nervous excited, uterine contraction has been reported and the like.

((Thunb.) Breit. Pinellia ternata ) ([...]) against the odor chew unusually the later but mucosal slightly if taste which aryl the neurointoxication is which enclose an properties and map. Since treating [...]shovel lower, seawater, headache due to containing which can be used for asthma, [...] , breast answer answer symptoms , brimmed conditions, vomiting, as a result of throat , I on the back a abscess, mastitis, pregnancy vomiting is used or the like. expectoration at pharmacological effect, considerably shaken, true vomiting, silicosis preventing, anti cancer activities and the like has been reported.

Wheat (wheat) (Triticum l. aestivum ) a mill is bonded. Wheat western but used as foodstuffs in formed with a thick is amount stock in, foodstuffs to 2 world together consumer's needs and. 90% lane breaking away is milling at least is crop, baking, confectionery, industrial. container is. Wheat bran which in addition also a starting material for soy sauce and toenjang a good feed is written. The blacked the durable and straw cap straw use cross-carried to or the like.

(Saururus Baill. chinensis ) blending ([...]) the leaves BB broke surface a savour of fish which kick slightly properties and map taste. Lower antipyretic, waste anti abscessdrainage action due to coughing, when spat defendant roentgen per hour at one meter , pneumonia, class chronic bronchitis , enteritis, urinary tract inflammatory winding, which wearing on abscess, when ball nail urine which generates a large heat is used. Antibacterial activity at pharmacological effect, immunoadjuvant effect, carcinogenic substances and, diuresis in, has been reported effect considerably shaken.

Won (Polygala tenuifoliaWilld.) does ([...]) the root at the end long permitted several circle stem is substantially provided to concentrate heap't vehicle. Leaves manner as to deviate from one another form of stripes and't is leaf sack and 1.5 ∼ 3 cm and lengths. The flower petal and is embedded with note-accommodated within an upper region of the outer brush end which make up for as. established. A [...] 2 (caryopsis) a flat, as-the seed which diverges it is born chock--a--supplied to the light. Herb in expectorant as polygalae radix roots, robust, . writing zero of popped rice seasoned with taffy liquid. Polyaddition Korean north and. distributed northern China.

The east Asia at least a fragment a (Gleditsia sinensis Lam.) a record head capable of going the improvement of the function field between the set minimum value and the various fasting treatment is out it from medicinal herbs used. A may be overcoated which called pieces, such as various of potent drainage. wearing on abscess. Korean. passing the winter even fat polyaddition.

Radix salviae miltiorrhizae ([...]) the unlined summer jacket (Salvia miltiorrhizaBunge.) is referred to to times ([...]), slightly smell specific medicinal and kick slightly which is astringent. Lower foot sole to stimulate blood circulation, for displaying sale subtotal and remove the high varies. mitigating joint pain. Wife physiological impure, cramps, partum visceral pain, as a result of fatherhonest submission -varies, .and treating obesity bruise. Due to heat of high temperature for psychoneurosis which [...] , nonsense, time trillion , insomnia, skin rash, a used and treating obesity palpitation. Pharmacological effect at coronary artery extension, decreasing cholesterol, blood pressure depressor, liver function activated, true in a multilamellar vesicle, anticancer, has been reported of antibacterial activity.

In the present invention using case ratio , volume hundred , astuteness , radix salviae miltiorrhizae, mint, against, wheat, blending, with water or an extract is pieces paper and using an organic solvent, such as ethanol a well-known conventional art method extraction can be extracted by.

(Mesenchymal Stem Cell, MSC) the lobe, the stem cell intermediate composition of the present invention to promote differentiation of by, the cells into which the fat make up or the blood it does not do room layer relative to the skin of a sense of volume to impart, skin external composition or number may be provided as pharmaceutical compositions,.

External skin a composition by the present invention number composition according to a body part, it is possible to transmit a, are specifically formulations thereof is not limited. Specifically for example, belonging to dicksoniaceae, nutritional wash containing a crude drug, massage cream, nutritional cream, pack, gels, or skin adhesive type cosmetic formulations of which can be cosmetic composition having, in addition lotion, ointment, gel, cream, patch or spray such as transdermal administration can be like dosage form.

case ratio composition number external the skin by the present invention, volume hundred , astuteness , radix salviae miltiorrhizae, mint, against, wheat, blending, paper and pieces 1 selected from the group consisting of phosphorous in the at least one useful as anti-skin external 0.0001-30 number % relative to the weight the total composition and comprising at amount of weight.

In addition each dosage the skin by external composition number, of the present invention said case ratio , volume hundred , astuteness , radix salviae miltiorrhizae, mint, against, wheat, blending, polygalae radix or extract is other than form or skin external preparation to other components according to a use purpose enemy without bothering a one skilled in the art can be combined which the selecting, in this case other material and connected to an external substrate. capable of effect moved up.

Furthermore, pharmaceutical compositions is a composition by the present invention may be used if the, preservative, stabilizer, hydrophilizers or emulsions promoter, salts and/or buffer osmolyte for pharmaceutical auxiliaries such as number and other therapeutically useful substances which may, according to conventional method various oral or oral dosage form formulated in penetration hole while moving up and down.

Purification for example formulations may be in the form of for oral administration, bolus, rigid and soft capsule number, liquid, suspension number, emulsion number, number syrup, granules or resonance is restricted by protecting the number, these formulations diluent in addition to an agent (e.g.: lactose, dextran [...] , sucrose, mannitol, sorbitol, cellulose and glycine) and [...] (e.g.: silica, talc, stearic and of magnesium or one of a calcium salt and of a polyethylene glycol) containing of wet liquid to flow down. Tablets in addition magnesium aluminum silicate, starch paste, gelatin, [...] estradiol, methylcellulose, sodium carboxymethylcellulose and binders such as [...] which agent, optionally starch, agar, alginate or the sodium salt such as disintegrant, absorbent, colored number, flavor number, and sweeteners such as can pharmaceutical additive. Tablets conventional mixing, granulation or coating can be produced by method. In addition of the dosage form for parenteral administration with injectable liquids or for formulation as a representative isotonic preferably is a fish. However, the severity of symptoms, for effective ingredient actual dose, routes of administration selected, of the subject age, sex, weight and state of health various fields including associated factor falling on a detector a to be determined must be understood, thus, also any dose constitutes a herb extract is provided to said method is not of, or limit the scope of the present invention. Generally herb extract is provided to dose constitutes a 0.001 mg/kg/day to approximately 2000 mg/kg/day is in the range of. Further the preferred dosage is 0.5 mg/kg/days to 2.5 mg/kg/day is.

Intermediates the lobe, the stem cell the present invention promote differentiation a cosmetic or molding of a composition for the application region includes all adipose tissue surface of the body, but not limited to specific site, for example, for liposuction the scan electrode driver applies a female breast, gas is injected may comprise an. Said fat tissue cosmetic or lower but increased adipose tissue purpose molding, this is not limited.

Hereinafter, a further embodiment the present invention and/or at least two different. rapidly and to reduce a memory. Stage, embodiment examples of the present invention relate to for only range of the present invention is not limited.

Herb extract is provided to [in the embodiment 1]

case ratio , volume hundred , astuteness , radix salviae miltiorrhizae, mint, against, wheat, blending, polygalae radix, at least a fragment a and male, blank, hundred two old Mr. , [...] , brain , [...] , flax user, 30g to ethanol each direction of repose (95.0-95.5v/v %), 200 ml then placed in an apparatus for ASE300 ACCELERATED SOLVENT EXTRACOR (DIONEX Corporation) in 50 °C 1500psi grinding under pressure was 20 minutes. In addition an apparatus for 87 sony ultrasonic cleaner (BRANSON Ultrasonics corporation) 3 in 50 °C was grinding additional degree. Each leave powder is 40 spindle module extract (Modul spin 40 ; Biotron corporation) an apparatus for 40 °C 24 in each drying time been produced with at sample extract.

[Test example 1] the lobe, the stem cell intermediate derived human an intramedullary nail into adipocytes stimulating growth efficacy and quantitatively measure

Human (human bone marrow mesenchymal stem cells, hBM-MSCs) the lobe, the stem cell intermediate derived an intramedullary nail theory company a (Lonza, Inc. Walkersville, MD, USA) according to instructions stored in the good theory company. Rosiglitazone trough instead (indomethacin) indomethacin, the guiding the adipocyte differentiation (Troglitazone) to an intermediate using fat cell the lobe, the stem cell of inducing differentiation of adenocarcinoma cancer cells a point except that the method using a recommended in theory company has been attained. Specifically, intermediate the lobe, the stem cell culture insulin 1 μg/ml, dexamethasone 1 µm (Dexamethasone, DEXA), 0.5 mm (isobutyl methyl xanthine, IBMX) this cow department[...] doesn't have any error frames, of differentiating into with a lipolytic stem cells by culturing has been, the adipocyte differentiation case ratio IDXT to when inducing ignition and under conditions, volume hundred , astuteness , radix salviae miltiorrhizae, mint, against, wheat, blending, polygalae radix or extract is added to medium at each 10 PPM. Furthermore, for comparison male, blank, hundred two old Mr. , [...] , brain , [...] , each extract direction of repose or flax added to medium at 10 PPM. Rosiglitazone trough positive the control group at using 2 µm (Troglitazone; TRO), was treating, such as badges IDX. Intermediate the lobe, the stem cell after 14 or not the program has instructed the starting the adipocyte differentiation, to remove the cell culture, (Phosphate Buffered Saline, PBS) buffer charged into the depositing tank of 10 ml of cells in a washing the then, 10% formalin/PBS solution 0.2 ml/cm² of cells using the 10 minutes. Fixed cells 60% isopropanol solution 0.5 ml/cm² 1 to which washes the times, a stain solution (Oil Red O) senses a rotation velocity of the disk means of isopropanol and 60% oil red five 0.2 ml/cm² lipid cell in fat minutes 10 to a dye of 100. Furthermore, a post-treatment effect such as the driving of a centrifuge and a shaker (hematoxylin) [...]refined salt in each to easily after the n bit parallel data inputted seen microscopically, case ratio , volume hundred , astuteness , radix salviae miltiorrhizae, mint, against, wheat, blending, polygalae radix or extract is also results of processing when showed to 1.

Also 1 a, IDXT or IDX intermediate under conditions of inducing differentiation of adenocarcinoma cancer cells than with fat cell the lobe, the stem cell IDXT of radix salviae miltiorrhizae extract under conditions further preparing a soil improving agent and a intermediate the lobe, the stem cell fat cell of inducing differentiation of adenocarcinoma cancer cells when the fat cell differentiation is more effectively occurred..

red five oil dyed after 100% isopropanol formulation then corresponding advertisement based on the shown measuring absorbance at a wavelength of 500 nm, showed to result to the computer of the also 2h to 2a. At this time, each data value was correcting the control group as insulin. This increase brings statistically significant corresponding advertisement based on the shown list, statistics corresponding advertisement based on the shown list verification tester to t [...] Stowe both sides, 0.05 hereinafter p value when that difference significant layer containing a filler. case ratio in addition, volume hundred , astuteness , radix salviae miltiorrhizae, mint, against, wheat, blending, polygalae radix, at least a fragment a, male, blank, hundred two old Mr. , [...] , brain , [...] , flax and intermediate when each handling a extract direction of repose the lobe, the stem cell 's growth rate stimulating growth fat contrast IDXT 4, 4'-methyl bix 30% or more, a table 1 showed to

Table 1 and 2a to 2h result of as is found in, case ratio by the present invention, volume hundred , astuteness , radix salviae miltiorrhizae, mint, against, wheat, blending, polygalae radix or extract is respective intermediate the lobe, the stem cell fat without inducing cell death, and to promote differentiation of cells which, male, blank, hundred two old Mr. , [...] , brain , [...] , flax or of repose direction extract and as compared to when a of fat cell more efficiently can promote differentiation may confirm it.

[Test example 2] skin sense of volume enhancing for measuring effectiveness of

case ratio of the present invention, volume hundred , astuteness , radix salviae miltiorrhizae, mint, against, wheat, blending, polygalae radix or extract is sense of volume of human skin in use of the article and to see enhancement is subject to centrifugal separation participants clinical trials of 15 the questionnaire to the embodiment.

2 table the specifically as defined in the each extract the in relation to the total weight the composition a 2.5% weight amount of daily cosmetic nutrient cream containing 1 microorganism beds can eliminate application site ball face daytime 8 corresponding advertisement based on the shown list, then records a response to the questionnaire for sense of volume for promoting the invention also provides for a. When the intention of a sense of volume growth promoting effect is, usually if the Δ, is charted to × mobile station if a, showed to 3 table to the result.

Said table as is found in 3, blank extract when the skin but sense of volume growth promoting effect from antenna, of the present invention while case ratio , volume hundred , astuteness , radix salviae miltiorrhizae, mint, against, wheat, blending, polygalae radix of the memory to which the extract at least a fragment or much of the test participants sense of volume the skin for growth promoting effect is. capable of confirming the.

case ratio said from said experiments, volume hundred , astuteness , radix salviae miltiorrhizae, mint, against, wheat, blending, polygalae radix or a composite containing the extract is each face applied to skin, test, and other tests that the dosage or invasive to the skin an effective ingredient of said extract is adipose tissue in a and so as to increase the volume, thus of skin effect-elasticity improving effect without sense of volume and recovery characteristics and possible to obtain can be viewed.

Preparations for the composition of the present invention to the exemplary thereby, the cold air flows.

[Formulation example 1] purification

The present invention according to case ratio , volume hundred , astuteness , radix salviae miltiorrhizae, mint, against, wheat, blending, at least a fragment paper and extract of species 1 in 100 mg, 400 mg lactose, maize starch 400 mg 2 mg magnesium stearic acid and after mixing, according to conventional manufacturing method of purifying was for preparing the tablets by granule with a sugar alcohol.

Number [formulations e.g. 2] capsule

case ratio , volume hundred , astuteness , radix salviae miltiorrhizae, mint, against, wheat, blending, at least a fragment paper and extract of species 1 in 100 mg, 400 mg lactose, maize starch 400 mg 2 mg magnesium stearic acid and after mixing, conventional capsule agent is filled with gelatin capsule according to the manufacturing method are also sealed have been prepared.

[Formulation example 3] belonging to dicksoniaceae (skin lotion)

[Formulation example 4] nutritional wash containing a crude drug (milk lotion)

[Formulation example 5] nutritional cream

[Formulation example 6] massage cream

[Formulation example 7] pack