PRODUCING METHOD OF SUPERCRITICAL SALICONIA HERBACEA L. EXTRACT HAVING ANTIOXIDANT AND ANTIMICROBIAL EFFECT
The present invention refers to and has an effect and antibacterial antioxidant extract of salicornia herbacea of manufacturing method relates to, more particularly a multistage auxiliary solvent supercritical carbon dioxide supercritical by extracting from salicornia herbacea extract of salicornia herbacea which has an effect and antibacterial antioxidant manufactured with an efficient and highly effective relates to method. Salicornia herbacea [...] of the our country ( National hydroxide microware filld for [...] of promotion circle according to component analysis, the water content leaf 90.9% in terms of specialized high school admission, 4.6% leaf the batch, stem is 6.1%, is 6.2% root. Salt content the stem highest memory state in selectively removed leaves, . sprout this of the roots, wherein the. During batch leaves content of. the paper side weft yarns to occupy about 70%. Each at the root the reducing sugar and total party 22.8% and 13.4% in terms of specialized high school admission and, uronic acid content of a sample (uronic acid) per leaf 0.31g 100g, stem is 1.40g, root is 1.88g. When leaf the amino acid (glutamic acid) glutamic acid, aspartic a large such as (aspartic acid), stem and at the root lysine (lysine), and the like. many glutamic acid. (Tyrosine) tyrosine the leaves is 10.8 mg/100g and to and roots according containing is not, the taurine denitrified 3-5 at the root stems, than leaves. many. pro phosphorus the leaf and at the root (proline) to about 7% total amino acid is detected. Inorganic sodium is best 1333.8 mg/100g is contains more. In particular contains more potassium [...] natriuretic is a catalyst, yet in the body drag and also calcium the maintenance of a healthy body of the framework of the canopy shelter is contains more. And highest memory state in the roots, wherein the sodium inorganic microware filld, calcium leaves (100g/650.1 mg) in root (22.1 mg/100g) about 20 is denitrified contains more. Magnesium (46.5 mg/100g -54.0 mg/100g) and potassium (650.1-741.1 mg/100g) according to large thin film transistors are electrically connected to a portion where heat collection, 29.6 mg/100g best the zinc stem in. high. 31.5 mg/100g electrode, an iron leaves, stem in 100g/66.2 mg, at the root 84.8 mg/100g in revealed which has, in addition copper, nickel, manganese and the like is containing traces. Chemically free radical (free radical) 1 one or more paired rotation undesirable electromagnetic fields but is present independent of with circulation promoted. species. Normal molecules 2 a opposite directions for in a three-shaft posture using are one pair of made exist stable, free radical of the pair prevent it form the active electron structure having generally unstable and thereby, very large and which is shorter in life. The generation of active oxygen in vivo various route generated. Normal metabolism processes even in 2% of oxygen mask is superoxide radical. are known to be converted into. After exposed to ultra-violet light, skin cells and tissues bisulphate cause free radicals damage to is skin device comprises an optical system damage consistent against the degradative action which presenting the, main physiological described as follows. (1) free radical to the skin and having an ultraviolet action generated. Skin free radical antioxidant defense system which contains a hardener, and a with each Certificate expires. If there are multiple amounts of ultraviolet and measures the audio defense meter and threshold is reached is induced oxide cells and tissues. As a result protein, lipid, the primary cells, such as DNA free radical components to be asked to damage by, the colouring substances and, decomposition of the surface during the cell functioning is promoted skin aging the skin damage and. is induced diseases. Free radical from the skin caused by the generation of a pleated three representative. users can. First, the skin by ultraviolet surface of the radioactive part into contact destruction antioxidant, second, lipid oxide, protein oxide, and DNA acids such as oxidation of matrix metalloproteases finally oxidation of component cell constituents overexpressing (matrix metalloproteinase), abnormal cleavage chain collagen cross-coupled, anionic cut chain (hyaluronic acid) connective tissue damage is found. Excessive ultraviolet exposure free radical produced by the corrugating which is able to promote various physiological damage as well as the resulting, a pigment present in the skin melanin stimulating cells (melanin) of the generating the skin for acceleration of constitution: known made be darkened. In addition according to the position and extent thereof of inflammation and serious skin immune of may be loaded with and without causing damage. An active referred oxygen kind harmful oxygen (toxic oxygen species) (reactive oxygen species, ROS) such as the breath by the action physiological the toxicity produced in cells, which is produced constantly material the extinction, in normal state, a to present 3-5%. This active oxygen kind super oxide radical (Superoxide radical, O2-), hydroxy radical (hydroxyl radical, HO+) free radical such as (free radical: chemically outermost electronic to and paired not atom or an acid molecules very unstable having reactive high) or in the form of hydrogen peroxide (hydorgen peroxide, H2 O2) a photointensifier include a photopigment which singlet or (Singlet radical,1 O2) such as a zero point pair present in the form of compounds with a. Physiologically active oxygen in-vivo sterilization but are respectively a defending activity, is eliminated oxide in vivo generally exact location of an diseases make the beneficial harmful. Attack a biological molecule active oxygen is the which damaging cells or tissues, involved in diseases adult to generate converted aging of an illness of from the received signals that reporting caused. Such activity by oxygen is which is capable of suppressing the oxidation antioxidants constitution: liquid silicon is cosmetic which either progressing actively effectively, extracted from Alaska Pollack potent natural aging research/development of units as simple processing steps at a low cost as prevention. Natural environment and yet show a growing from natural plant material a variety of physiologically active component containing antioxidant and excellent having function inputted from the user, such physiologically active based the food, cosmetic, and by winding the pharmaceuticals. However, physiologically active of natural plant royal tomb antioxidant and method for extracting step of greater extent according to is governed degree due to the excellent friendly, high-vivo in blood of the physiologically active ingredients in a brewing operation the most suitable for the user the method establish a non-volatile memory elements is considered critical, as they of wet liquid to flow down. The extraction solvent extraction or extraction heat most is received by the in-vehicle came. After extraction is comprised of a conventional, heat sensitive caused loss of those components, a and the contents stored in the database on the state of charge organic solvent, the solvent is removed, and separation effect low oil component is connected to the semiconductor layer. improve power efficiency. Other active ingredient (supercritical fluid extraction) supercritical fluid extraction extraction of wet liquid to flow down process is used. The material a predetermined portion of liquid phase of operation is set to a pressure and temperature or more dissolution of viscosity and the spreading factor of gas phase force having a characteristic the, quickly and selective extractive method is. Supercritical fluid extraction in particular used in cost-effective carbon dioxide as the solvent, is nontoxic force of the stitched area of the properties such as extraction solvent environment-friendly is shown and are potentially useful in the applied. Under such background, the present inventor in blood of the physiologically active various extract of salicornia herbacea are as a mask and effects utility value of dot type considered there is insufficient in the supercritical carbon dioxide and a ground drying salicornia herbacea step co-solvent supercritical which is then subjected to reverse phase extract of salicornia herbacea said a message based on biological evaluation skin in vitro of human skin cells in vitro safety, antioxidant activity DPPH, antimicrobial ability against microorganisms deterioration atopic dermatitis and evaluates it result in particular from salicornia herbacea having excellent well as antimicrobial activity antioxidant activity possible to obtain an efficient and highly effective extract checks that the he rattled through his the present invention. The present purpose of the invention a supercritical fluid a multistage auxiliary solvent supercritical fluid extraction is which has an effect and antibacterial method for producing salicornia herbacea by a rope. provides. Said to solve the, including a the present invention refers to the following steps which has an effect and antibacterial antioxidant extract of salicornia herbacea of provides manufacturing method. 1) 60 °C to 35 °C ground drying salicornia herbacea 100bar to 250bar under a pressure of at temperature and of supercritical carbon dioxide supercritical fluid extraction difference be debinded the step of agitating the mixed hot metal 1 (step 1); Drying salicornia herbacea extracted supercritical difference 2) said 1 said 1 ground tea extract and while maintaining the same temperature range of 450bar to 300bar while the pressure increasing to a process pressure in the supercritical carbon dioxide and a co-solvent supercritical fluid extraction difference 2 to remaining maintaining component and to remove impurities (step 2); and Drying salicornia herbacea extracted supercritical difference 3) said 2 said 2 ground tea extract and while maintaining the same temperature range under final pressure behaviour of said step 2 in the supercritical carbon dioxide and a co-solvent to the supercritical fluid extraction difference 3 which has an effect and antibacterial antioxidant extract of salicornia herbacea, methods of recovering (step 3). Preferably, of the present invention which has an effect and antibacterial antioxidant extract of salicornia herbacea of the manufacturing method 1 a the following steps prior to said step b to step may further comprise the: A) step of drying a salicornia herbacea (step a); and B) said salicornia herbacea building for grinding step (step b); Preferably, of the present invention which has an effect and antibacterial antioxidant extract of salicornia herbacea of the manufacturing method after said step 3 extract of salicornia herbacea obtained in said step 3 (step 4) a concentration step may further comprise the. Preferably, of the present invention which has an effect and antibacterial antioxidant extract of salicornia herbacea of said step 4 after the manufacturing method step said extract of salicornia herbacea concentrate obtained at the 4 to filter out, and recovering the supernatant (step 5) may further comprise the. Hereinafter, the present constitution of the invention described the detail the. In the present invention, said supercritical extraction supercritical fluid extraction process (supercritical fluid extraction, SFE) to which means that, generally a supercritical fluid is high-pressure conditions a high-temperature gas in the critical point having only memory, a random access memory properties of liquids and gases has, chemically a non-polar solvent with similar polarity the nature has pump of a fat-soluble supercritical fluid is possible to.. When a driver fluid equipment supercritical carbon dioxide pressure and critical point liquid and gaseous through a process having both properties of supercritical fluid thus fat-soluble solute solubility increased. Supercritical carbon dioxide is maintains the amount of material containing extraction vessel is allowed to pass through the fat-soluble material contained in the sample retention and covers the other variety of materials are. out extracted by supercritical carbon dioxide. In particular maintaining component is extracted from that scene and is separated from and removal of fall a by the host device. Maintaining component raw material remaining in the extraction vessel which is then subjected to reverse phase (defatted raw material) small amount of co-solvent back to flowing supercritical carbon dioxide containing pure passing extracted signals Q5 and Q6, following readout supercritical carbon dioxide extracted components are not obtainable can be permitting. Through such as in particular the present invention refers to said method, and covers the other retention falling somewhat active removed and, defatted is separated from pure supercritical carbon dioxide signals Q5 and Q6, following readout purity an active substance which has not been extracted is connected to the semiconductor layer. be possible that the frequency of the. the step a, as a step in of drying a salicornia herbacea, extraction of salicornia herbacea in order to increase the efficiency from the preprocessing circuit is. A term used in the present invention "salicornia herbacea ( In the present invention, its various said salicornia herbacea the engine or partially (for example leaf, flower, root, stem, of, seeds, and the like, and dehydrating are performed) means, preferably leaves, stem, of, and mixture by the addition of an initiator or roots from shell, . mixture by the addition of an initiator stem most preferably. In the present invention, said air ventilation has a step may be performed in a drying furnace, not limited to blood. A and hot air-dried said using hot wind in meaning manner, preferably at a temperature range of 40 °C to 70 °C but may be performed, is at. During days a and hot air-dried in addition said, preferably 1 to 3 which can be performed during, this moisture content of salicornia herbacea can be reducing the driving time of a compressor. Said step b the, building for grinding as a step in said salicornia herbacea, by building salicornia herbacea extraction of salicornia herbacea pulverizing unit in order to increase the efficiency from the preprocessing circuit is. In the present invention, said a salicornia herbacea particle size of 0.5 mm to 2 mm preferably grinding the. If out-of-range of the grinding particles when said size, salicornia herbacea of extraction efficiency, and decorative use and the like.. In particular, in grain size of less than 0.5 mm load pressure when super critical extraction wall with a extraction efficiency due to 2 mm in size larger particles is discharged, containing a little particles efficacious material in supercritical fluids on resulting difficult are extracted is formed in the outer pump of effective. efficiency. In the present invention, starting at said milling method can be a fin can be is subjected to stripping for stripping. Said step 1 the, 60 °C to 35 °C ground drying salicornia herbacea 100bar to 250bar under a pressure of at temperature and of supercritical carbon dioxide as the supercritical fluid extraction difference step of agitating the mixed hot metal be debinded 1, of relatively low pressure in pressure conditions grinding drying salicornia herbacea as an antimicrobial component and antioxidant effects except retaining component is mainly, and recovering the extracts. Maintaining said included in salicornia herbacea which are representative of a component lipid and fat. lipid-soluble substance such as. Is the present invention they are in other words, an antioxidant and anti-microbial active ingredient and a low content of, inhibiting the activity and antibacterial antioxidant acting as can be. In the present invention by be debinded them as by performing a supercritical fluid extraction difference 1, maintaining component outputted at high efficiency can be removed from the. Said step 1 under a condition of maintaining said anti-oxidant and anti-components as an antimicrobial component gap is formed between the substrate support can be extracted an amount of.. 3 step future through second pattern is formed by a well as antimicrobial activity of an antioxidant in efficiently obtained component. Step 1 including a retaining component extracted from the supernatant may be processed to recover extract after executing removing purpose according to use. Preferably, said step 1 are preferably designed as 10 minutes to 1 time, more preferably 20 minutes to 40 ingredient, most preferably about 30 minutes but may be performed, not limited to blood. Of the present invention in one embodiment, the 1 50 °C pressure temperature of said step for setting an e -150bar supercritical carbon dioxide using only the embodiment 30 minutes. The 2 said step, said 1 drying salicornia herbacea extracted supercritical difference said 1 ground tea extract and while maintaining the same temperature range of 450bar to 300bar while the pressure increasing to a process pressure in the supercritical carbon dioxide and a co-solvent supercritical fluid extraction difference 2 to remaining maintaining component and as to remove impurities, as well as remaining salicornia herbacea said step 1 in other words, an antioxidant and as an antimicrobial component except for the impurities. is and recovery. The 2 step said further, active a non-constant component extraction for a multi-steps (step 3) and the pressure and temperature the aim of set so as to utensils, photopolymerization initiator represented by chemical of intermediate steps. In the present invention the dopants will said as an antimicrobial component and an antioxidant desired antioxidant except for various as is the inhibition of the activity and antibacterial, which are representative of a is retaining aforementioned can be. Removing said step 1 mainly component maintained at small component 2 in the following steps including antioxidant well as antimicrobial activity which are able to inhibit the components, i.e. the objective compound. to remove impurities. Specifically, connected to the said 2 step 1 and step 1 step said stepwise act as intermediate steps of 3, step 1 from pressure extraction of pressure comparatively high pressure, 450bar to 300bar condition and within the range of to a process pressure while slowly increasing pressure can be. In addition step 2 a supercritical carbon dioxide supplied during the course of a further a co-solvent can be supplying is melted or the outside. The scanner by the wafer transfer residual raw material through that significantly removes and brightness and can be, further in a subsequent step 3 purity antioxidant and antibacterial component capable of extracting can be adjusted to values very. Temperature of said temperature in said step 2 step 1 but may be the same as, and not limited to 60 °C in a temperature range from 35 °C to be. In the present invention, step 2 the pressing speed preferably 2 bar/min to 25 bar/min can be. 1 as well as said step, step including impurities extracted from 2 is recovered and allowed to extract after executing removing purpose according to use. Preferably, said step 2 preferably has a to 10 minutes to 1 time, more preferably 20 minutes to 40 ingredient, most preferably about 30 minutes but may be performed, not limited to blood. Of the present invention in one embodiment, the 2 step said (Ethanol) ethanol as co-solvent while adding be. In the supercritical fluid extraction device, to maintain the temperature and the pressure is from 50 °C 150bar 400bar up while slowly increasing the ethanol co-solvent extraction with is rinsed in the supercritical carbon dioxide by flowing out of pairing a predefined 30 minutes performed for all the. The ethanol used as co-solvent supercritical fluid extraction device of the wall remaining oils (maintaining component) until. that the guide. The 3 said step, said 2 drying salicornia herbacea extracted supercritical difference said 2 ground tea extract and while maintaining the same temperature range under final pressure behaviour of said step 2 in the supercritical carbon dioxide and a co-solvent to the supercritical fluid extraction difference 3 which has an effect and antibacterial antioxidant extract of salicornia herbacea, and recovering the as, said step 1 and step 2 is the inhibition of the activity and antibacterial antioxidant through formed in the center of crushed article of drying salicornia herbacea removed substantial amount of an antioxidant in and antibacterial active ingredient-purity compound in a is vehicle speed is lower than the extracted and with high efficiency. Preferably, said step 3 are preferably designed as time 5 minutes to 30, more preferably 60 minutes to 3 time, most preferably about 120 minutes but may be performed, not limited to blood. Of the present invention in one embodiment, the 3 said step (Ethanol) ethanol as co-solvent while adding be. In the supercritical fluid extraction device, to maintain the temperature and the pressure is 50 °C 400bar while maintaining the co-solvent extraction with ethanol is rinsed in the supercritical carbon dioxide by flowing out of pairing a predefined 120 minutes performed for all the. Preferably, said supercritical carbon dioxide 1 step 3 to step 5 ml/min to 60 ml/min which can be supplied with a volumetric flow rate in, extraction time contrast greater amount of for anti-oxidant purpose by good volume of 30 ml/min to 60 ml/min to supply is preferably but, not limited to blood. Said volumetric flow the supercritical liquid carbon dioxide from a carbon dioxide bombay sewage up to desorption of the carbon dioxide in the high-pressure pump and the pressure and temperature state is based on a volume, per unit of time. volume and into extraction bath. In a term "co-solvent" in the present invention using the supercritical carbon dioxide for use with organic solvent mixture by the addition of an initiator, anti-oxidant and anti-absolute as an active ingredient, an in of more efficiently as an antimicrobial component can be outputted at high.. Using type solvents comprise trialkylamines ethanol, methanol, chloroform, water, ethyl acetate, hexane and with D ethyl ether one or selected from the group consisting of at least one 1 2 may be used in mixture of, preferably, use can be made of, ethanol. Said step 2 and step 3 of the entire co-solvent mixtures with supercritical carbon dioxide by stably of 1 to 30 volume % is usable in, preferably 5 volume % amount of use the preferably in extraction efficiency. The 4 said step, said extract of salicornia herbacea obtained at the 3 step a concentration as a step in, extracted extract of salicornia herbacea in a co-solvent extract to remove. is the step of concentrating. A typical known art the concentrate said marker for the diagnosis of gastric cancer for concentrating. that can be actuated to effect. The 5 said step said extract of salicornia herbacea concentrate obtained at the 4 step to filter out as, and recovering the supernatant, extract of salicornia herbacea concentrate of precipitation in green bottom during high a purity excluding, and recovering the supernatant is as an active ingredient. The final extract in the supernatant portion antioxidant and antiaging active is better than. Specifically, the various publicly known supercritical extract of a filter can be performed using, paper filter involves the use of optical signal and an electric signal filtration efficiency but preferred, not limited to blood. Antioxidant of the present invention which has an effect and antibacterial extract of salicornia herbacea of retaining-c region and/or greater manufacturing method for removing impurity of a step for by 1 and step 2, and the dyeing steps extract in 3 step 1 and step 2 antioxidant activity than extract in higher well as antimicrobial activity could confirm it. In in particular of the present invention in the embodiment, in the case of supercritical fluid extraction of the present invention active and antimicrobial antioxidant activity reduces blood cholesterol, platelet of the existing method unusually high compared to could confirm it. Specifically, to experiment of the present invention, having excellent antioxidant activity extract of salicornia herbacea of the present invention manufacturing method according to (experiment example 1), has (experiment e.g. 2) antimicrobial activity, further adding specific concentration cytotoxic (experiment e.g. 3) and the amorphous layer not having any has been confirmed. Furthermore, the present invention refers to said method extract of salicornia herbacea produced by including as an active ingredient for preventing or ameliorating skin aging provides a cosmetic composition. The aforementioned manufacturing method extraction via at the extract of salicornia herbacea obtained is high in comparison with the thermal hot-water extract of the existing method as described above, has antioxidant activity are effective in and wrinkle-reducing skin whitening, the skin aging cosmetic composition for preventing or ameliorating can be used. Furthermore, the present invention refers to said method extract of salicornia herbacea produced by including as an active ingredient for preventing or ameliorating atopic provides a cosmetic composition. The aforementioned manufacturing method extraction via at the extract of salicornia herbacea obtained hot-water extract of the existing method has monocyclic beta-lactam antibiotics are is high in comparison with the thermal are effective in atopic, the cosmetic composition for preventing or ameliorating atopic can be used. A term used in the present invention, "prevention" rotation, administered to the subject composition of the present invention said skin an effect of aging or suppress generation of atopic all or delay. behavior. A term used in the present invention, the "improved" the treated state parameters associated with, for example all the at least reduced degree of conditions. behavior. A term used in the present invention, the RAT "individual", livestock, including mammalian including human or the like. all animals. In the present invention, cosmetic composition for example, wash containing a crude drug, skin, lotion, cream, gel, ointment, paste, powder foundation, immunopathological, emulsion foundation, seat belt, paste, powder, spray, water-in-oil or oil-in-water (O/W) (W/O), such as a can be formulated into dosage forms, and.. Furthermore, the present invention according to the cosmetic composition in cosmetic for mulatins said acceptable carrier may comprise an. Wherein, "cosmetic for mulatins in acceptable carrier" that can be included in a cosmetic for mulatins email widow, a web page or already publicly known compound or composition that be is used in forward or the compound or composition may be developed are generated when the contact with the skin of the as adaptable abnormal toxicity of, . of the mobile communication network people, Letters and the thing which or irritative instability. Said carrier relative to the total weight of said cosmetic composition of the present invention about 1 weight % to about 99.99 weight %, preferably about 90 weight % to about weight of the composition, of a body is included to the display apparatus 99.99 weight %. However the ratio said produced cosmetic composition is of the present invention, according to formulations aforementioned passed it on to an application region of the specific (face, such as neck) a preferred application or its which differ depending on the amount and the like because the, said any ratio whatever side of the present invention involves limiting the range is don't understood. On the other hand, said carrier as alcohol, oil, surfactant, fatty acid, silicon oil, wetting, moisturizing number, viscous modified number, emulsion, stabilizer, UV blocking agent, chromophore number, can be the exemplary flavoured. Said alcohol, oil, surfactant, fatty acid, silicon oil, wetting, moisturizing number, viscous modified number, emulsion, stabilizer, UV blocking agent, chromophore number, fragrances compounds which can be used in already, or the like, that has/composition since the publicly known art if one skilled in the art/composition material information by local and selecting suitable, use can be made of, the. Of the present invention in one embodiment, the present invention according to the cosmetic composition in addition to extract of salicornia herbacea said glycerin, butylene glycol, propylene glycol, polyoxyethylene-hardened castor, ethanol, triethanolamin may include or the like, preservative, [...] , dye, purified water need of may include trace according to. Furthermore, fat substances and further contains cosmetic composition of the present invention, organic solvent, dissolved number, gelling and number concentrate, softening number, antioxidant number, point that serves as a suspending agent, stabilizer, blowing agent (foaming agent), aromatic, surfactant, water, W emulsion or non-ionic-glucose, an oligosaccharide, a number, charging number, WIPO chelating agent, preservative, vitamin, blockers, damp topic , essential oils, dye, pigment, hydrophilic or lipophilic active agent, lipid vesicles or cosmetic at the any other, such as a component cosmetic diagnostics or dermatological field typically used in can an auxiliary agent. And, the components of the commonly used in dermatological field may for example have been introduced an amount. An extractor that is aforementioned method extracts through a extract of cosmetic composition having which the exemplary application, if one skilled in the art, within the range of the present invention, extract of salicornia herbacea antioxidant effect, skin whitening and wrinkle-reducing based on, skin, which can be drinking scalp and shampoos and such as soap or detergent, food and beverage can be material and poured or the like, is in addition the present invention must understand made to. The present invention refers to using supercritical carbon dioxide and a co-solvent antioxidant from salicornia herbacea in multiple stages by extracting is rinsed in the supercritical extract well as antimicrobial activity, antioxidant derived salicornia herbacea an efficient and highly effective extraction of extract well as antimicrobial activity that can provide the method, including extract said skin whitening and wrinkle improving effect for makeup to effect improvements atopic composition or a cosmetic composition is provided which. the. Figure 1 shows a supercritical multi-stage manners of the present invention embodiment also obtained through extraction monitoring Taxus cuspidata erase radical according to DPPH of agricultural extract kit article is of graph representing the result. Figure 2 of the present invention embodiment supercritical multi-stage the first deoxygenator Hereinafter, embodiment to the present constitution of the invention and effects, to one specifically, than, these embodiment relate it will be a record only an exemplary of the present invention in the embodiment of the present invention range only limited not. In the embodiment 1: multi-stage crushed drying salicornia herbacea supercritical fluid extraction Anti-oxidant and anti-from crushed article of drying salicornia herbacea in a multilamellar vesicle high activity small component can be efficiently obtained by multiple stages through supercritical fluid extraction method on an entire surface of the semiconductor, low activity, the oil (maintaining) for removing been includes degreasing. Each of the in an extraction step an extract obtained both are obtained as sample. Is pre, and hot air-dried during 1 in 60 °C a salicornia herbacea pin style pulverizer after 0.5mmm using particle size of raw material extracted by crushed to shown. First step to, 150 g each of said pre-processed raw material implanted amount of supercritical fluid extraction device (SCFE-0050-0100-080-re-ISA, puts [...] , Korean) 50 °C in using, the 150bar pressure difference 1 embodiment the supercritical extraction. 60 ml/min in the flow rate of supercritical carbon dioxide 30 minutes corresponding advertisement based on the shown list extraction (SFE extraction), is recovered and allowed to extract wherein are obtained as sample (compared e.g. 1). Pressed in a second step to, first and the subsequent step of supercritical fluid extraction device 400bar from 150bar by controlling the pressure of and pressure slowly until, as well as supercritical carbon dioxide of ethanol as co-solvent (ethanol) further the 1 tea extract through the tea extract for embodiment 2 of the ingredients. The first step and the second step to the flow rate of supercritical carbon dioxide remains the same state with, co-solvent 3 ml/min in ethanol 30 minutes the flow rate of extraction corresponding advertisement based on the shown list, is recovered and allowed to extract wherein are obtained as sample (compared e.g. 2). Third step the to, in 50 °C 400bar pressure in the supercritical carbon dioxide and a co-solvent in the range of ethanol is supplied to an engine, said 2 tea extract through for tea extract 3 of the ingredients (SFE with co-solvent) the embodiment. Third step the fluid flow to a each one of the fluids of the aforementioned pressed in a second step and equal to 10 Ps, corresponding advertisement based on the shown list extraction minutes 120, is recovered and allowed to extract wherein are obtained as sample (in the embodiment 1). Respect to said each stage, supercritical fluid extraction device supercritical fluid extraction system when the contents are executed supercritical is formed on the resultant structure pressure bath extraction of the fluid condition that were as a sample extract. Said samples for each stage the withdrawal of a treatment fluid and using for the concentration of the concentrate and a. Said 3 tea extract of the resulting in the embodiment 1 (Whatman filter papers No. 1) paper filter samples for concentrating the excited. Furthermore, samples that were each. These for each table a showed to 1. Experiment 1 e.g.: supercritical fluid extraction condition analysis experiment ability erase radical DPPH extract according to In said in the embodiment 1 to which the extract obtained above is relative to the sample experiment erase radical DPPH the embodiment. The ethanolamine 1, 1-Diphenyl-2-picryhydrazyl on said present test method (DPPH, Sigma D9132-1G) produces free radical by testing ability to an erase method of a nonvolatile direct effect antioxidant user indicates the functionalities, is a test method. Compound 1, 1-Diphenyl-2-picryhydrazyl the ethanolamine (DPPH, Sigma D9132-1G) and generate the free radical in, the, having a concentration of the extract by mixing a amount of free radical it is found out that whether reduced to some extent. Specifically ethanol 0.4 ml to 0.5 ml solution DPPH of 0.1 mm, at a predetermined concentration and dilution is added a 0.1 ml and extracts from the homogeneous strongly seconds. 10 after vortexing leucorrhea cow in reacting a metal salt of 30 minutes, using ELISA measures the absorbance in 517 nm, antioxidant using ethanol degree of potency light absorption burglar percentage based on group of a garnish layer containing a filler. The resulting expressions of the following ability by removing free radicals the bill by 1. [Expressions 1] (%) = {1-(B/A)} ×100 active by removing free radicals A: process a sample extract absorbance well with the control group 1 having no B: process a sample extract absorbance well group the experiments Result to the computer of the a table showed to 1 and 2. Said result through the erase radical DPPH increases is enhanced concentration in the embodiment representing high capacity can be confirm that the user. Experiment 2 e.g.: measuring and analyzing apparatus of antimicrobial activity hot-water extract extract and in the embodiment Activity of extracts of supercritical salicornia herbacea a signal modulation unit in order to of solid medium for utilizing a antibacterial test is performed for all the. To this end a typical negative bacterial gram Solid medium for utilizing a antibacterial test, i.e. to accomplish a agar plate paper difusion assay together with the information performed for all the. Result to the computer of the also showed to 2. Also 2 through the, : a hot-water extract of salicornia herbacea Experiment 3 e.g.: concentration of extract in the embodiment by human keratinocyte forming cells cytotoxic evaluation for measuring stability Said in the embodiment to which the extract obtained above is cytotoxic by concentration of a sample by sample evaluation for measuring is performed for all the. In the embodiment 1 supercritical as a beverage for a human skin root sheath cells comprises a in other words, keratinocyte (keratinocyte) HaCaT cell line and anionic additives proliferation-promoting effect and security for using test MTT were examined. To this end human keratinocyte type characteristic taxgun week 24 well plate to HaCaT cells 5 × 103 cells/well at equipment through the inlet at the hemacytometer the same after the good frequency divides the. 10% FBS in DMEM containing by culturing time 48-50% by surface area of the culture vessel, the cultured surface, salicornia herbacea supercritical extract the processed in a concentration appropriate 24 hours' extra good. Culture nor post-incubation 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT, Sigma M5655, USA) solution (2.5 mg/ml) for 50 micro l adding good further time 3. Furthermore, off all cell culture, 200 micro l of dimethyl sulfoxide (DMSO, Sigma D2650, USA) per each well after the l micro 200, 100 micro l of 570 nm to take 96 well it was determined that the absorbance in Enzyme-Linked Immunosorbent Assay (ELISA). Safety upon a cell and anionic additives promoting the proliferation using pure water effect the control group of absorption strength percentage based on layer containing a filler. Result to the computer of the a table showed to 3 and 3. As a result, in the embodiment the lowest extract 0.0001 weight % in concentrations processing allowing an cells in a high resolution PSD so that the developing is observed concentration or more process, but he acid to increase cell proliferation in been effect is observed. Not cytotoxic and is thereby can be acyl. Experiment 4 e.g.: HPLC analysis result the first deoxygenator index component embodiment Supercritical fluid extract of the present invention said in the embodiment 1 (Betain) betaine component index corresponding advertisement based on the shown list is selected, betaine to HPLC extract (Betain) respect to the contents of fixed human power by operating all systems. Time of a steam heater and then 5,000 PPM of said in the embodiment 1, to 0.45 µm membrane filter filtering the was subject to analysis by HPLC (waters, USA). Analysis as at the columm SHODEX sugar KS-802 (8.0 mm × 300 mm, 5 µm, USA) using the n bit parallel data inputted, bed using calcareous water human power by operating all systems by corresponding advertisement based on the shown list 50 minutes. Flow rate 1 ml/min, the dose l micro 10, column temperature of 84 °C, detector was to analyze the contents to refractive index difference to RI Detector. Result to the computer of the to table 4 and a also showed to 4. Also and a 4 4 through said table, supercritical multi-stage of the present invention extract of salicornia herbacea obtained through extraction is containing kitchen beta substantial amount of is can be viewed. The present invention relates to a producing method of a Saliconia Herbacea L. extract having an anti-oxidant and an anti-microbial effect and, more specifically, to a producing method of a Saliconia Herbacea L. extract having an anti-oxidant and an anti-microbial effect at high efficiency from the Saliconia Herbacea L. through multistage supercritical extraction using supercritical carbon dioxide and a co-solvent. COPYRIGHT KIPO 2016 The following steps including a which has an effect and antibacterial antioxidant extract of salicornia herbacea of manufacturing method: 1) 60 °C to 35 °C ground drying salicornia herbacea 100bar to 250bar under a pressure of at temperature and of supercritical carbon dioxide supercritical fluid extraction difference be debinded the step of agitating the mixed hot metal 1 (step 1); 2) drying salicornia herbacea extracted supercritical difference said 1 said 1 ground tea extract and while maintaining the same temperature range of 450bar to 300bar while the pressure increasing to a process pressure in the supercritical carbon dioxide and a co-solvent supercritical fluid extraction difference 2 to remaining maintaining component and to remove impurities (step 2); and 3) said 2 drying salicornia herbacea extracted supercritical difference said 2 ground tea extract and while maintaining the same temperature range under final pressure behaviour of said step 2 in the supercritical carbon dioxide and a co-solvent to the supercritical fluid extraction difference 3 which has an effect and antibacterial antioxidant extract of salicornia herbacea, methods of recovering (step 3). According to Claim 1, said co-solvent ethanol, methanol, chloroform, water, ethyl acetate, hexane and with D ethyl ether 1 selected from the group consisting of one or at least one mixture with the method 2. According to Claim 1, said co-solvent a supercritical carbon dioxide and co-solvent mixture at a low temperature of 1 to 30 volume % to characterized by method. Extract of salicornia herbacea produced by any one of Claim 1 to Claim 3 method as an active ingredient including skin aging cosmetic composition for preventing or ameliorating. Extract of salicornia herbacea produced by any one of Claim 1 to Claim 3 method as an active ingredient including cosmetic composition for preventing or ameliorating atopic. Number The extraction temperature (°C) Extraction pressure (bar) Extraction time (min) Co-solvent (ethanol) Compared e.g. (tea extract 1) 1 50 150 30 1 difference 2 difference 3 difference: total 180 ingredient - Compared e.g. (tea extract 2) 2 50 150 400 from pressurized until 30 3 ml/min In the embodiment 1 50 400 120 3 ml/min By concentration in the embodiment Control 62.5 g/ml micro 125 micro g/ml 250 micro g/ml 500 micro g/ml 1 mg/ml (%) Active by removing free radicals 0 6.7 10.7 17.3 27.9 41.8 By concentration in the embodiment Control 0.0001 weight % 0.0005% weight 0.001 weight % 0.005% weight 0.01 weight % Relative cell growth (%) also 100 89.8 123.6 108.3 121.6 135.2 In the embodiment 1 extract of concentration In the embodiment 1 of extract concentration betaine 5,000 PPM 62.316 PPM
