MANUFACTURING METHOD OF RICE BRAN OR BARLEY BRAN FERMENTED MATERIAL CONTAINING GABA, AND RICE BRAN OR BARLEY BRAN FERMENTED MATERIAL CONTAINING GABA PRODUCED BY MANUFACTURING METHOD
The present invention refers to pulse river or rice bran containing will pasteyeast manufacturing method and manufacturing method of produced by pulse river or rice bran containing will pasteyeast relates to material, is production capacity pyrrolecarboxamides more specifically of Enterococcus faecium MMD-11 ( Generally the rice rice bran in rice unpolished rice rice hull takes size, and silk screen printing to the product in opposite direction at a process circulation promoted. inside leisure. Such rice bran an iron, calcium, magnesium, vitamin E inserting program is obtained from which amino acids including, increasing memory and the improvement of the function brain cell to improve the concentration thereby reducing fat neutral cholesterol and even preventing adult diseases are known as HMG-COa reductase potent. In addition from the process of barley pulse river[...] and cosmetics is. Such rich in high protein and that produces less fiber pulse river, constipation, obesity activity, various effects such as skin beauty, thereby preventing adult diseases which are known as HMG-COa reductase potent. 4 the world barley consumer's needs and belonging to grain to grain important long in our country which eating habits we of color cathode material can be extensively used, silk screen printing barley consumer's needs and such as bran and each remaining after said pulse river excellent nutritional configuration and at least two images is used as feed most current despite, other than. which is not comprises use. Thus chamber is low said bran and pulse river valuably from functional food material produced. acid techniques. The pyrrolecarboxamides while [γ-aminobutyric acid (GABA)] distributed contains thermoplastic resin and correct tertiary structure of proteins in animal one of amino acids in the case of brain, heart, elongated, waste such as neurotransmitter preconditions central nervous system is found in the paper side weft yarns to occupy a about 30%. Plant in green tea, cabbage traditional our country detected in roots kimchi fermented food is contains more even. An increase in blood pressure and have and of the brain blood flow and preventing obesity, for improving vision, anti anxiety, anti improved in the function of brains, and convulsions, dementia prevention of a polyimide resin, such juice physiology effective in disorders whose extraction is rendered exhibits the best brains food (brain food) bonded. In particular for formed by developing holders [...] as well as functional material having menopausal or andropause generated during sleepless, depressive, stable neural to the lateral center part of the anxiety is output from the speaker as sound effect encloses the core functional material that has. Associated with the prior art literature call patent number 10-1373252 registration 25th which at in brown rice guava leaf yeastyeast guava leaf brown rice and manufacturing method of the molecular weight of brown rice guava leaf fermentation powder difference is formed integrally with the screw and disclosure for manufacturing method, without using implying pulse river or rice bran, rice bran and portulaca oleracea in call opening patent number 10-2014-0046865 25th which containing a fermentation composition and its manufacturing method is formed integrally with the screw and disclosure, but using rice bran pyrrolecarboxamides production capacity strains that are pyrrolecarboxamides an apparatus for compensating for a non-in first-out part receives the channel estimation may be significantly degraded content. Therefore, the present invention refers to yeastpulse river or rice bran is for manufacturing the same are fermented to, pyrrolecarboxamides production capacity strains that are by the addition of, the pyrrolecarboxamides-rich yeast material manufacturing method and manufacturing method produced through forging by use-obesity activity, will paste potent stress pulse river or rice bran containing yeast. material. The present invention refers to said of the prior art such as one of the upper and lower surfaces and if a bit in the complimentary invention, is of Enterococcus faecium MMD-11 pyrrolecarboxamides production capacity ( Said is used as a signal mark the present invention refers to MSG to achieve, pulse river or rice bran, water a blending step; said mixture obtained through mixing step 100-130°C, and sterilization and to sterilising a minutes 10-20 1.2-1.8 pressure in; said sterilization stages mixture cooled to a cooling step 40-45 °C; said cooling step into a mixture functional lactic acid bacteria starter via the vaccination step vaccination 0.05-0.1% (w/v); stages vaccination said mixture in a proofing step fermentation time 70-74 25-35 °C; obtained through proofing step said fermentation product of centrifugal separator minutes 10-30 11,000-13,000rpm in a centrifugal separation steps; said centrifugal separation step to obtain a in the fermentation broth obtained through freeze-dry to includes a powdered, said vaccination step of Enterococcus faecium MMD-11 28-32 °C manner in a medium free of MRS ( By the present invention a tap water rather than distilled water and said containing will paste pyrrolecarboxamides a yeastpulse river or rice bran is production capacity of Enterococcus faecium MMD-11 ( The employing vegetable through natural material thereby will pasteyeastpulse river or rice bran containing an isocyanate group can be obtained in material, and of producing high content of pyrrolecarboxamides yeast. can be produced. In addition high pyrrolecarboxamides content-obesity activity due [...] due to a failed normal stress and. having high efficacy. Nutritional thereby, effective excellent will pastepulse river or rice bran containing yeast by providing a material which a component can contribute to the for improving the health of.. In addition are utilised uses many undesirable bran and pulse riverpulse river related industrial to bran and by utilizing decreased and the environmental pollution is prevented.. Also according to an embodiment of the present invention Figure 1 shows a pulse river or rice bran containing will pasteyeast of order indicating a manufacturing method. Figure 2 of the present invention in the embodiment 1 according to pH of rice bran containing will pasteyeast graph indicates a change in the flow. Figure 3 of the present invention in the embodiment 2 according to pH of yeastwill pastepulse river containing graph indicates a change in the flow. Also of the present invention in the embodiment 1 according to Figure 4 shows a rice bran containing will pasteyeast graph indicates a change in the flow of acid. Also of the present invention in the embodiment 2 according to Figure 5 shows a containing will pastepulse riveryeast graph indicates a change in the flow of acid. Figure 6 of the present invention in the embodiment 1 according to rice bran containing will pasteyeast pyrrolecarboxamides of graph indicating a content. Figure 7 of the present invention in the embodiment 2 according to pulse river containing will pasteyeast pyrrolecarboxamides of graph indicating a content. Figure 8 of the present invention in the embodiment 1 according to of obesity activity of rice bran containing will pasteyeast efficacy of graph. Figure 9 of the present invention in the embodiment 2 according to pulse river containing will pasteyeast efficacy of graph of obesity activity. Figure 10 of the present invention in the embodiment 1 according to rice bran containing will pasteyeast efficacy of graph of stress. Figure 11 of the present invention in the embodiment 2 according to stress of containing will pastepulse riveryeast efficacy of graph. Hereinafter, a preferred embodiment of the present invention. as further described focuses. These embodiment relate more specifically, the present invention for, these range rights of the present invention only limited not in the embodiment. Door appended 11 also to 1 by the present invention consults a pulse river or rice bran containing will pasteyeast manufacturing method and manufacturing method of produced by pulse river or rice bran containing will paste to the coverstock yeast a subsurface look detail the configured the first deoxygenator embodiment can be mass-produced. By the present invention containing will pastepulse river or rice bran yeast of MSG (Monosodium Glutamate) first manufacturing method, pulse river or rice bran, water the rotational operation done on the rotary a blending step (S10). the mixing step 100 parts by weight water, MSG 0.5-1.5 15-25 pulse river or rice bran and mixed parts by weight of a.. The glutamate has said MSG [...] dicarboxylic rightly by material which is switched, said MSG is 0.5 parts by weight if less than a etching pulse river or rice bran containing will pasteyeast pyrrolecarboxamides of content can be lowered and, exceeds the 1.5 parts by weight of said MSG, photoresist is longer after decomposition by constitution time without degrading. In addition said pulse river or rice bran pulse river or rice bran containing will pasteyeast three categories, the substrate is rotated to form a are fermented in manufacturing material, said 15 parts by weight of rice bran or pulse river without mounted on a are fermented if less than, 25 parts by weight of fermented switches exceeds the longer after fermentation that are not problem is connected to the semiconductor layer.. Said mixing step 100-130°C mixture obtained through, and sterilization and to sterilising a pressure 1.2-1.8 10-20 minutes in the rotational operation done on the rotary (S20). Said sterilization stages a cooling fluid for cooling to 40-45 °C mixture is then subjected to a step (S30). Said cooling step into a mixture functional lactic acid bacteria starter via the vaccination 0.05-0.1% (w/v) vaccination step (S40) the rotational operation done on the rotary. The pyrrolecarboxamides step is production capacity said vaccination of Enterococcus faecium MMD-11 28-32 °C manner in a medium free of MRS ( the Enterococcus faecium MMD-11 can be isolated from soybean paste and the traditional. In addition said vaccination step (S40) in functional lactic acid bacteria starter by seeding a less than 0.05%(w/v) pyrrolecarboxamides surface and can be lowered production capacity, if the load gradient exceeds the first 0.1%(w/v) [...] switches longer not been generated, a content item in a received smart constitution. Vaccination said stages in a fermentation time 70-74 25-35 °C mixture is then subjected to a proofing step (S50). Said obtained through proofing step fermentation product of centrifugal separator minutes 10-30 11,000-13,000rpm in the rotational operation done on the rotary (S60) a centrifugal separation steps. Obtained through separation step to obtain a centrifugal said in the fermentation broth freeze-dry to a powder step (S70) of a signaling transfer point, said pulse river or rice bran containing will pasteyeast of the fabrication is completed. Said read state to the read state is or polyisocyanurate foams will pastepulse river or rice bran containing a yeast stores at a temperature of-1-5 °C. Hereinafter said pulse river or rice bran containing will pasteyeast to for manufacturing the same, said Enterococcus faecium MMD-11 strain and isolation of identifying and, using the same yeastwill pastepulse river or rice bran containing e.g. an experiment using a computer to making of the, . as further described focuses and test in the embodiment. < e.g. experiment > The present invention refers to is of Enterococcus faecium MMD-11 pyrrolecarboxamides production capacity ( [Material and method] (1) of Enterococcus is production capacity pyrrolecarboxamides from 2004 (Enterococcus pulse river or rice bran containing will pasteyeast to enhance the functionality of Enterococcus ( (2) (Enterococcus Strain genomic DNA from strain candidate for identifying a DNeasy Blood & Tissue kit (QIAGEN, Hilden, Germany) separated the using. DNA was along the needle point of a communication network and downloads one is obtained in tablets of. 16S rDNA Primer for cloning of the 5 '-AGAGTTTGATCMTGGCTCAG-3' (Forward) and a 5 '-ACGGGCGGGTGTGTRC-3' PCR amplification using (Reverse) was enforcing. 100ng mold DNA PCR amplification, DNA of primer 0.5 µm, 2 mm dNTPs, 10X reaction buffer solution and a Taq polymerase 5U/micro l (Doctor protein, Korea) in 95 °C using modified 30 seconds, 30 seconds annealing (annealing) in 55 °C, 72 °C Biometra thermocycler for 35cycle 1 in a incremental stretch-ingredient (Tampa, Florida, USA) was amplifying. PCR product a combination of a winding vibration 1% agarose gel electrophoresis it is found out that the embodiment. Identified through PCR product 16S rDNA sequencing MMD-11 bio-sequence listing of obtained information. Obtained 16S rDNA sequence information either in NCBI (www.ncbi.nlm.nih.gov) using corresponding advertisement based on the shown list exposure showing a homology of DNA, the for comparing and analyzing homology with bio-sequence listing. (3) containing will pastepulse river or rice bran yeaststarter for manufacture of soybean and the conventional ring-back (Enterococcus Strains separated sugar fermentation pattern and bio-chemical characteristics for irradiating an API 50 CHL kit (BioMerieux sa, Marcy-L ' Etoile, France) was according to a the needle point of a communication network and downloads. (Lee, J. S. , K. C. Lee, J. S. Ahn, t. I. Mheen, Y. R. Byun and Y. H. Park. 2002. (4) manufacturing yeastpulse river or rice bran containing will paste will pastepulse river or rice bran containing a manufacturing yeastpulse river or rice bran 1% MSG + 20% embodiment and Enterococcus faecium MMD-11 high pressure sterilization ( (5) [...]analysis L micro 800 pyrrolecarboxamides analysis (methanol: chloroform: water = 12:5:3 (v/v)) a solvent 200 micro l yeast of mixed with sample material. Centrifugal separator, a mixed solution prepared (12,000rpm, 4 °C, 15 ingredient) by the n bit parallel data inputted recovery difference 1 supernatant, and layer, organic in solvent layer chloroform and water (1:2 (v/v)) mixing 600 micro l mixed solution after centrifugation the supernatant, and is collected to the difference. 1,2 2 difference that were deflected supernatant the n bit parallel data inputted freeze-drying, by removing particles and ultra pure water obtained by dissolving 0.45 micro m PVDF film by passing HPLC analysis sample at night. HPLC (Waters, Milford, MA, USA) derivatized with 6-aminoquioly-N-hydroxysuccinimidyl carbonate (AQC) sample for analysis the n bit parallel data inputted, 3.9x150mm AccQ, TagTM (Nova-PakTM C18, Waters) column selecting signal derivatives have been isolated (Seok, J. H. , K. B. Park KB, Y. H. Kim, m. O. Bae, m. K. Lee and S. H. Oh. 2008 Production and characterization of Kimchi with enhanced levels of-aminobutyric acid. [Result] (1) containing will pasteyeastpulse river or rice bran for manufacture of a conventional starter (Enterococcus pulse river or rice bran containing will pasteyeastwill paste from 2004 including a starter for manufacture of generating blue bromophenol to separate the lactic acid bacteria separated added with ring during colony-burn symptom at an medium MRS without formation of a thick 9y extension of candidate those was selecting strain. Candidate the probability generating pyrrolecarboxamides of a metal oxide fiber illuminated the TLC. Excellent productivity pyrrolecarboxamides through TLC strains was selecting. Enterocyte-strain at 2004 derived MMD-11 morphological of results investigating the characteristics and biochemical separated gram positive strains Streptococcus power is fed, separated lactic acid coconut-L strains, D-loss, such as galactose-D acid for example, to decompose to and, inositol, [...], such-L a disassembled on a tube pump (table 1 reference). Enterococcus faecium MMD-11 derived 2004 (table 1) ( Ampholyte + (positive) in said table 1, -represents a voice (negative).. By identification of strains (2) 16S rDNAsequence analysis Enterocyte-strain analyzing the 16S rDNA bio-sequence listing the for identifying. < in the embodiment1aodirenc> EnterococcusfaeciumMMD(Enterococcus Water 100 parts by weight, and 20 parts by weight of a MSG 1 of rice bran the. derivative to obtain a mixture. 121 °C said mixture, in sterilization a pressure 1.5 15 minutes. Said. cooled to 45 °C a mixture of sterilized. Enterococcus faecium MMD-11 said cooled into a mixture for 30 °C manner in a medium free of MRS ( Said functional lactic acid bacteria starter is vaccinated with the mixture in 30 °C are fermented to obtained a fermentation product of time 72. 12,000rpm in said fermentation product of 20 minutes the obtained for adding the centrifugation. Said fermentation broth to freeze-dry the powder by, rice bran containing will pasteyeast of the fabrication is completed. < in the embodiment2aodirenc> EnterococcusfaeciumMMD(Enterococcus Water 100 parts by weight, and 20 parts by weight of a MSG 1 pulse river the. derivative to obtain a mixture. 121 °C said mixture, in sterilization a pressure 1.5 15 minutes. Said. cooled to 45 °C a mixture of sterilized. Enterococcus faecium MMD-11 said cooled into a mixture for 30 °C manner in a medium free of MRS ( Said functional lactic acid bacteria starter is vaccinated with the mixture in 30 °C are fermented to obtained a fermentation product of time 72. 12,000rpm in said fermentation product of 20 minutes the obtained for adding the centrifugation. Said to freeze-dry the fermentation broth by the powder, containing will pastepulse riveryeast of the fabrication is completed. <1aodirenc e.g. test > pHchange In the embodiment 2 in the embodiment 1 and said alcohol to the glycolide monomer at between 3 after innoculation strain to fermentation by date when it was determined that pH change. A change in pH 6.5 difference of said in the embodiment 1 0 1 difference of next the reduced from this a massage cream, an essence, 5.88 5.06 low up until the difference 3 (also reference 2). In addition said in the embodiment 2 of a change in pH 1 0 difference 6.04 power is fed 3 with being burned variations that experiences a larger difference 4.82 low up until the difference (reference 3 also). <2aodirenc e.g. test > floor In the embodiment 2 in the embodiment 1 and said alcohol to the glycolide monomer at between 3 after innoculation strain to fermentation by date when it was determined that floor. A change in the acidity of said in the embodiment 1 1 3 power is fed 0.32 difference increased to thereby cause in difference (also 4 reference) have been shown to 0.75. In addition said in the embodiment 2 1 difference a change in the acidity of a massage cream, an essence, this 0.31 0.49 3 have been increased until the difference (also 5 reference). <3aodirenc e.g. test > pyrrolecarboxamidescontent In the embodiment 2 in the embodiment 1 and a produced in said strain to 3 after innoculation pyrrolecarboxamides when fermentation between it was determined that content. In the embodiment 1 and in the embodiment 2 generates a time has passed an increase in content pyrrolecarboxamides increases can be viewed (also 6 and 7 reference). The employing vegetable through the natural material containing will pastepulse river or rice bran yeast the process for forming the material, high content pyrrolecarboxamides yeast that it is possible for making of the can be viewed. <4aodirenc e.g. test > obesity activityefficacy In the embodiment 2 in the embodiment 1 and a produced in said strain to 3 after innoculation made by fermenting the strain between 3T3-L-1 cells after it was determined that efficacy obesity activity. Fat cell as said 3T3-L-1 cells, also reference to 8 said 3T3-L-1 Control not also which processing cells (control), treated only rice bran said 3T3-L-1 cells representing the result of bran and said 3T3-L-1 yeast rice bran in said in the embodiment 1 cells presenting the material a rice bran yeast surfaces of a material, said measured 3T3-L-1 cells of rice bran in levels in relation to the Control show differences and the pulse widths of the great the main a yeast said Control step more than offsets said rice bran show and low is numerically evaluated and the. In addition also by referring to 9 said 3T3-L-1 Control not also which processing cells (control), treated only pulse river said 3T3-L-1 cells representing the result of pulse river and said 3T3-L-1 yeastpulse river in said in the embodiment 2 cells presenting the material a pulse riveryeast surfaces of a material, measured 3T3-L-1 cells levels in relation to said in the embodiment 1 as well as in said said Control pulse river show differences and the pulse widths of the great the main a yeastpulse river said Control step more than offsets said show and low is numerically evaluated and the. Thereby said pulse riveryeastyeast material rice bran (in the embodiment 1) and the (in the embodiment 2) material by suppressing the accumulation and a fatty the adipocyte differentiation to be efficacy obesity activity can be viewed. <5aodirenc e.g. test > stressefficacy Said alcohol to the glycolide monomer at in the embodiment 1 in the embodiment 2 and 3 after innoculation strain relative to stress cells following made by fermenting the strain between it was determined that efficacy. Also reference to 10 said Control not also which processing cells (control), treated only rice bran said cells representing the result of bran and said cells of rice bran in said in the embodiment 1 strains that are production capacity pyrrolecarboxamides to a to be inoculated with a a presenting the material yeastwill paste surfaces of a rice bran, measured in cell survival ability to show differences and the pulse widths of the great the rice bran said said Control said rice bran said Control step more than offsets the main [...] show and than the reflective. Reference to 11 also in addition said Control not also which processing cells (control), said cells representing the result of treated only pulse riverpulse river and said cells strains that are production capacity pyrrolecarboxamides in pulse river in said in the embodiment 2 to be inoculated with a a yeastpulse river a presenting the material surfaces of a will paste, measured cell viability in said said in the embodiment 1 as well as said Control pulse river show differences and the pulse widths of the great pulse river[...] said Control step more than offsets the main said show and than the reflective. Thereby said pyrrolecarboxamides pulse river pyrrolecarboxamides of rice bran (in the embodiment 2) (in the embodiment 1) and on upper and a viability when stress resistant to stress and uniformly adjusts to be efficacy can be viewed. The present invention relates to a manufacturing method of a rice bran or barley bran fermented material containing gamma-aminobutyric acid (GABA), and a rice bran or barley bran fermented material containing GABA produced by the manufacturing method. The manufacturing method of a rice bran or barley bran fermented material containing GABA comprises the steps of: mixing MSG, rice bran or barley bran, and water; sterilizing the mixture; cooling the mixture; inoculating a functional lactic acid bacteria starter in the mixture; secondarily fermenting the mixture; centrifuging the fermented product; and pulverizing the fermented liquid through lyophilization. COPYRIGHT KIPO 2016 with pulse river or rice bran, a blending step (S10) MSG and water; said mixture obtained through mixing step 100-130°C, and sterilization and to sterilising a minutes 1.2-1.8 10-20 in pressure (S20); said sterilization stages 40-45 °C mixture cooled to a cooling step (S30); said cooling step into a mixture functional lactic acid bacteria starter via the vaccination 0.05-0.1% (w/v) vaccination step (S40); said stages vaccination 70-74 25-35 °C mixture in a proofing step fermentation time (S50); said obtained through proofing step fermentation product of centrifugal separator minutes 10-30 11,000-13,000rpm in a centrifugal separation steps (S60); said centrifugal separation step to obtain a in the fermentation broth obtained through freeze-dry to a powder step (S70) including a containing will pastepulse river or rice bran yeast of manufacturing method. According to Claim 1,100 parts by weight water phase minute description said mixing, said MSG 0.5-1.5 parts by weight of, or rice bran pulse river 15-25 parts by weight of a mixed pulse river or rice bran containing a will pasteyeast of manufacturing method. According to Claim 1, said pyrrolecarboxamides is starter functional lactic acid bacteria phase minute description vaccination is production capacity ( According to Claim 3, said step said vaccination ( Produced by any one of Claim 1 to Claim 4 method will pastepulse river or rice bran containing yeast material.
