EXTERNAL SKIN APPLICATION COMPOSITION WITH ANTI-INFLAMMATORY AND ANTI-AGING EFFECTS, CONTAINING ZOSTERA CAPRICORNI PLANT CULTURE EXTRACT AND PRODUCTION METHOD THEREOF
The present invention refers to well the blood ( Of human skin, and at inner product over a time that the various control metabolism decreases and high yield and releasing hgrf to the hormone, immune cell function and cell of required and authenticating biometric becomes poisoned with a configured and in vivo protein immunomodulatory the biosynthesis of proteins endogenous aging (intrinsic aging) and a player, extracellularly at various contaminants and ultraviolet by photoaging (photoaging) by, which thin skin (in the case of endogenous aging), installed rotatably on the base panel of the housing body elasticized a topical patch preparation is exposed to and keratinocyte in skin by UVB by melanin cells is subjected to cell damage. Skin aging is not exposed to the sun in aging regiment crane occurring in the site (chronologic aging) can take place in the exposed parts of the solar and a of change in regiment crane composited with aging (actinic ageing) aging- demoniac is prevented is array block, and by dividing 6. Clinical characteristic in aging in regiment crane fine clients ask skin wrinkle, atrophy of dermis, is the blood it does not do room layer second material is observed. Photoaging system controls an the pleat deep rough (coatse wrinkling and furrowing) is revealed and abnormal accumulated amount have elasticity (elastotic material) material such as leather is the skin is thick. is substantially slackened. Chronic sunlight epoxy skin in a transmission the nozzle in one resilient first locking slide member in misplacing the collagen blown together of dermis amount have deposition of material (solar elastosis) is increased (proteoglycan) proteoglycan and they are hundred qualities of an IGBT dermis collagen is dramatically reduced the recording operation.. Imparting tension strength and to the skin collagen the outer which force or stimulation of serving for the protection of the skin against the paper side weft yarns to occupy 90% layer dermis a reduction of the collagen is output from the speaker as sound more closely with aging skin associated have a relationship of wet liquid to flow down. Endogenous cells in or aging skin constituting a, vertically active cell photoaging, signal delivery system had been incompletely decomposing the dermal tissue, an enzyme in the biosynthesis of MMPs (matrix metalloproteinases) is increased in, in the biosynthesis of collagen to discharge a gas from an interior, is reduced in the resilient them and mushroom kimchi, skin melanin formation on a wheel and installs a sensor 5c are known as HMG-COa reductase is increased. Thus for propagating or skin cells, skin constituting a matrix substance the skin, by increasing of of materials that can, a substrate configured skin collagen material MMPs for decomposing an aromatic the compression chamber volume can be suppressed in the biosynthesis of a material, which is capable of suppressing the melanine biosynthesis, wrinkle substance, resilient loss, blackening skin such as skin to alleviate symptoms. has been shown to be. Whitening, in anti-aging, in a multilamellar vesicle for constitution: liquid silicon is coated according materials but, existing chemical materials toxicity, vocoded, use a plurality of leads and excessive dosage according to various problems such as side effects to accumulate the inputted energies used as the side effects thereof and the like natural, a difference between both scales is to develop a material in plants. brisk sufficient extracting active ingredient. However natural safety even when, electrochromic stability possibility on cosmetic of pharmaceuticals effective beyond a threshold concentration in many of the dangers in using the subject is, a satisfactory effect objects' traces to couldn't. On the other hand, adapted to seawater marine it blooms wellphanerogam of accident plants that our country numeral key the them a total of 8 well the blood form of wet liquid to flow down. Yan it blooms well high fecundity based on valued economical to ecosystem the seawater and many marine animal to feed, habitat and part or the like spawning ground Yan improved fecundity hydroxide of the notches has a very important in addition which serves, in sea water by through tissue of the aerial part and nutritional salt and remove, are promoted tissue rosanin and water of stabilizing such very for the purification environment Yan which one or an important role, well blood research the beginning the lenses only a few ten years in Korea a massage cream, an essence, should not outside environment including the objects' traces to study as a kind of electric charge like. Vegetable constitution cosmetic development and functional food in the same by using resources of the protein complex from a using plepuring for material has been but, regard well in the bloodwell the blood extract in a cosmetic composition techniques patent preceding used active ingredient (patent document 1) part of the shaft, into a plant cell culture therefrom, provided to or extract thereof culture irritation studies have plepuring for cosmetic material is levels no. The present inventor are well the blood plant cells, irritation culture or extract thereof is in a multilamellar vesicle, anti-aging, and can be used as effective in whitening, and the like, and, he rattled through his the present invention. Therefore, the present purpose of the invention the well the blood ( In the present invention, for improving wrinkles for improving skin said, whitening, skin inflammation, inhibitors, for improving, inhibiting sebum secretion, improved inhibit or acne, preventing damage the skin by ultraviolet, mitigating, relief, for includes. Another object of the present invention, said well the blood plant cells, irritation culture or extract thereof to show excellent skin improved composition for external application for skin number is provided to manufacturing method. Another object of the present invention, nucleotide sequence of active proliferation well the blood said method is provided to and transplant method. Said to achieve is used as a signal mark, the present invention refers to plant cells of ( The present invention refers to in addition, ( The present invention refers to in addition, ( The present invention refers to in addition, ( The present invention refers to in addition, ( The present invention refers to in addition, ( The present invention refers to in addition, ( The present invention refers to in addition, ( The present invention refers to in addition, including following steps ( (A) of well the blood, or stem tissue irritation or plant cells are separating the culture a; and (b) said well the blood plant cell cultures or irritation culture composition for skin external application containing or extract thereof number composition the stages of formation of the product. In the present invention, said (a) step, (I) said well the blood are separated from each other and tissue or stem of [...] benzyl 6 - (6-Benzylaminopurine, 6-BAP), 2,4-dichloro phenoxyacetic (2,4-Dichlorophenoxyacetic acid, 2,4-D) containing cellulose in a broth medium by culturing the transformed plant cell cultures to obtain an or, well the blood (ii) said tissue or stem of (IBA) butylic acid indole are separated from each other and containing cellulose in a broth medium by culturing the transformed to obtain an culture irritation, Can be characterized in that the. In the present invention, said (a) step in culture, , 3 to 6 time or a UVA, shikimic acid for 150uM-400uM culture added to medium further including manner. In the present invention, said (b) step, (a) obtained in a well the blood plant cell cultures or irritation of drying and culture, powder mixed water composition producing or, powder is mixed water, ultrasonic extraction or reduces blood cholesterol, platelet including step of preparing the composition manner. The present invention refers to, including following steps, ( (A) nucleotide sequence of active well the blood fragment with a extraction the leaves or stem; (B) light fragment with a leaf or stem 0.5-1 mg/L 6-benzyl [...] (6-Benzylaminopurine), L 2/0.3-0.6mg, 4-D, containing life times MS medium (Murashige and Skoog) in amount by number 1 to obtain an plant cell cultures well the blood anti according to; (C) containing [...] 0.2-0.5 mg/L MS the culture medium to which said (b) step for plant cell cultures well the bloodlife timesnew candle by inducing an amount; and Derived from (d) said (c) step new candle hydroxybutyric of 0.2-0.5 mg/L (containing MS Indole-3-butyric acid (IBA) by amount life times manner in a medium free of the step of inducing root. Bacterium obtained by the said present invention refers to in addition said method for steel or for sticking optical fibers in sea well the blood to well the blood provides method characterized by transplantation of plant. The present invention according to well the blood plant cells containing the same extract or culture irritation or external application to skin having number toxic to composition of the present invention for treating a skin wrinkle using cold not fall dead in spite of, pore-shrinking, skin whitening, pin inhibitors, acne inhibitors, using ultraviolet rays having such as lotion, lotion, skin damage. well the blood plant proliferation in addition the present invention according to. propagating through method. Adult well the blood electrophotographic Figure 1 is indicative of the scene of a plant. Figure 2 is electrophotographic indicating a process proliferation plant well the blood. Also the present invention according to Figure 3 shows a well the blood is electrophotographic into a plant cell oil plants. Also the present invention according to Figure 4 shows a oil plants well the blood into a plant cell a 5 L in bioreactor is electrophotographic indicating a process in culture. well the blood ( The present invention refers to in one aspect, well the blood ( In the present invention, rotation "for improving skin", condition on the inside of skin, e.g., when clause salt, mitigation of inflammatory, preventing, reducing, anti-aging, for treating a skin wrinkle using cold, whitening, pore-shrinking, skin elasticity effect through synergistic action of, protected the skin by ultraviolet, sebum inhibitors, . including both such acne. In the present invention, meaning of "diseases which contain as the active ingredient" a, skin external number various said composition and an improvement effect of a skin, for example, wrinkle, whitening through ability antioxidant, efficacy in a multilamellar vesicle such as effective amount of the quantity. containing a. In the present invention, the plant cell cultures well the bloodwell the blood part nucleotide sequence of active, e.g., stem, or leaves all or some of an induction cut in the. of a product that is prepared. The culturing such induction of the relates to tissue culture, small for the photomodulation of living tissue culture within plant tissue ( "Plant cell cultures" cut in plants the texture or culturing cellulose in a broth medium containing oxindole, what type of plant of a wound and, or processing oxindole a at nine caused in a tissue or an special circulation promoted. cell mass. Usually callus cell cancer, a normally for tissue differentiation or forming engine causing ability, amorphous tissue or cell mass and which is the specific portion cytometry. Agrobacterium include a broad definition (agrobacterium) caused by infection such as as to include the organization chart tumor plant. also. Therefore, the present invention according to well the blood stem, leaves such as being derived from body legs but, and is capable of providing both is, in one embodiment, some tissue dried by stem well the blood, or a seed germination well the bloodtype grave are guided against the cotyledonous from may be into a plant cell, the cut part whether tissue which well the blood derivat ives and cytochrom oxindole placing the culture medium to which the controlling the content kinin a face, plant cells may be formed. In the case of culture irritation and plant cell culture but predetermined, just, irritation can be derived to. different combination plant hormones. In the present invention, said culture irritation or plant cells well the blood the total way of the composition or extract thereof 0.1 to 10 weight % relative to the weight may. Only this ratio and the user makes a is a desired range, e.g., a 0.1 weight % to 5 weight % in the embodiment in demonstrated that despite instances is formed integrally with the screw and includes a, 5% or more, 10% capable of mediating to have good corresponding advertisement based on the shown list it is confirmed that, cell survival rate, not affected by even identifying improving in addition 10%. In addition to is 20%, 30% or more even in the case, and an improvement effect of a application to skin having excellent, wrinkle, whitening, in a multilamellar vesicle function. nontrivial twiddle factors and to one skilled in the art have. In the present invention, in a multilamellar vesicle a ultraviolet rays blocking said, anti-aging, for treating a skin wrinkle using cold, whitening, skin elasticity effect through synergistic action of, protected the skin by ultraviolet, sebum inhibitors, includes for inhibiting acne. In the present invention, said (embryoid bodies) culture irritation or plant cells well the blood a pigmented or self, is either used to filter out culture, is either used the cultures, the second powder is dried to cultures, use can be made of, for melt water purification. In the present invention, "extract" well the blood the extract culture irritation or plant cells or a pulverulent material, cold water extraction, heat extraction, ethanol, such as extraction oil jojoba various conventional extract obtained by extraction.. Extraction method in the present invention is not limited the first substrate are assembled, for example leucorrhea needle extraction, ultrasonic extraction, reflux extraction, reduces blood cholesterol, platelet, such as extraction oil jojoba. Wherein, in the case of reduces blood cholesterol, platelet, 8-48 time in distiller scalding, hot-water extract to obtain heated to 80-100 °C. In the case of extracting cold water, e.g., cold water (15-25° C) to said culture itself or by mixing powder drying thereof in extracting copper 3 to obtain pipe into the cold water extract. Or, water, organic solvent, or in a solvent mixture of an aprotic using the can be produced in a method. Immediately a extracted or or concentration and/or drying especially wet particles, use can be made of, the. When extracting using an organic solvent, methanol, ethanol, isopropanol, butanol, ethylene, acetone, hexane, ether, chloroform, ethyl acetate, butyl acetate, dichloro methane, N, N-dimethylformamide (DMF), Dimethylsulphoxide (DMSO), 1,3-butylene glycol, polypropylene glycol or a combination of organic solvent as the solvent employ the crude drug effective components of said is attached to or minimized at conditions to produce. can be extracted by hot or room temperature. Extracting organic solvent extraction for effective ingredient of the medicament according to examine itself loss of the insertion part and the of non-materials, by finding the an organic solvent and subjected to normal suitable outputs a relay driving signal.. In particular, ethanol extract in the present invention, preferably 20-50% ethanol extract, as one embodiment 50% ethanol extract preferably, extraction method as described the embodiment aspect, after mixing the 50% ethanol to obtained by stirring into copper 3. In the present invention, said extract is concentrated and, or high the dilution, use is made of an electrolyte distillation of extract may be loaded with. The present invention refers to in another aspect, (Zostera marina.) well the blood of plants containing the same extract or culture irritation or plant cells for skin improvement for external application for skin number composition provides manufacturing method: (A) of well the blood, or stem tissue irritation or plant cells are separating the culture a; and (B) said well the blood plant cell cultures or irritation culture composition for skin external application containing or extract thereof number composition the stages of formation of the product. Said (a) step extracted from well the blood material is woven portion, plant tissue culture, i.e., plant cell cultures, or irritation is process obtaining cultures. In the present invention, said nucleotide sequence of active leaves well the blood, or stem tissue, use can be made of, are separated from each other and. In the present invention, said separated from the tissue in plant cell cultures, irritation, for cultures an appropriate medium may be selected. Said (a) step, specifically well the blood plant cells or irritation medium suitable for culturing may be selected. In various technical fields as normally used in a tissue culture if there is a medium, .used without limited. Plant generally MS medium, and by using the mixture or the like medium B5, in one example, MS of an overlay medium, composition (1L fiducial time) the NH4 NO3 1650 mg, KNO3 1900 mg, CaCl2. 2H2 O 440 mg, MgSO4. 7H2 O 370 mg, KH2 PO4 170 mg, KI 0.83 mg, H3 BO3 6.2 mg, MnSO4. 4H2 O 22.3 mg, ZnSO4. 7H2O 8.6 mg, Na2 MoO4. 2H2 O 0.25 mg, CuSO4, 5H2 O 0.025 mg 88800013038 882. 6H2 O 0.025 mg, FeSO4. 7H2 O 27.8 mg, Na2 EDTA.2H2 O 37.3 mg, Myoinositol 100 mg, Nicotinic acid 0.5 mg, Pyridoxine-HCl 0.5 mg, Thiamine-HCl 0.5 mg, Glycine 2 mg, Sucrose 30000 mg is. Derived plant leaf, when inducing ignition and plant cell culture back of a human derived plant stalk, basic MS media composition the difference in substantially cannot, plant cells, most important in inducing irritation of plant growth hormone on the kind of. hereinafter. The present invention according to the case of plant cell culture, in the case of plant cell culture derived from or stem leaves, (Benzylaminopurine) (6-BAP) [...] benzyl 6-, 2,4-dichloro (Dichlorophenoxyacetic acid) phenoxyacetic (2,4-D) is made of the by culturing the transformed plant cell cultures can cause. The ratio combination of each hormone 6-Benzylaminopurine (6-BAP) and 2,4-Dichlorophenoxyacetic acid (2,4-D) is 1 : 0.4-0.8, or 1 : 0.5-0.6 can be characterized in that the. I.e., 6-benzyl [...] (6-BAP) 100 parts by weight, 40 to 80 parts by weight 2,4-dichloro phenoxyacetic, or 50 to 60 weight manner wife. E.g., 6-benzyl [...] (6-Benzylaminopurine, 6-BAP) and 2,4-dichloro phenoxyacetic (2,4-Dichlorophenoxyacetic acid, 2,4-D) 0.5-1mg/ml each, and 0.3-0.6 mg/ml can be containing. While, in the case of culture irritation, indole hydroxybutyric (Indole-3-butyric acid, IBA) is made of the cultures irritation by culturing the transformed and may be obtained. E.g., a discriminating signal of a comparator is 0.2-1mg/ml IBA can cause culture irritation. In the present invention, said (a) step in culture, , 3 to 6 time or a UVA, shikimic acid for 150uM-400uM dispersed by a blender before being inoculated in addition to medium may further include any. In the present invention, whitening, anti-aging, in a multilamellar vesicle, sebum inhibitors, improved acne, face on left and right side parts the skin by ultraviolet in order to increase the efficacy of, attracting number processing may include a process. UVA processing number attracting such processing, shikimic acid, including processing which phenol-type compounds, carotenoid, flavonoid increase. in addition. Detailed conditions as follows. 1. UVA: as physical attractant UV-A fluorescent lamp (20W, Sankyo Denki, Japan) a daily 0.5-8 hours a and by applying treatment culture a time. 2. Shikimic acid: 200-600 M MS of shikimic acid (Shikimic acid) packed in culture the medium. In the present invention, in said (b) step, through culture of step (a), the mixed paper has excellent air cell or irritation including a second culture composition number for skin external use of the correct shape producing or composition, or said cultures with radio frequency form extract through publicly known method obtained by the composition number for skin external use of the correct shape can be produced. E.g., said (b) step, (a) obtained in a well the blood of drying and culture irritation or plant cells, mixed water powder composition producing or, (A) obtained in a well the blood of drying and culture irritation or plant cells, powder is mixed water, cold water extraction, reduces blood cholesterol, platelet, oil jojoba using vegetable oils including an extractor that is, inorganic solvent including or ethanol using of an organic solvent such as alcohol using the composition the stages of formation of the product may comprise an. In another example, culture obtained in said (a) a hot air after drying in order to carry out the method water by powdered can be produced. In addition the present invention according to well the blood plant cells, irritation culture or extract thereof has excellent melanogaster generating inhibition ability, whitening by utilizing. can be. In addition the present invention according to well the blood plant cells, irritation culture or extract thereof may be utilized by the pore-shrinking for.. In addition the present invention according to well the blood plant cells, irritation sebaceous secretion inhibitory culture or extract thereof, improved inhibit or acne, using ultraviolet rays have a lotion, lotion, skin damage. In addition the present invention according to well the blood plant cells, irritation iNOS blood cholesterol by inhibiting the activity of the culture or extract thereof through-inlfammation, mitigation of inflammatory i.e., relief, has a effect such. In the present invention, said skin external composition number cosmetic composition. can be, or in pharmaceutical compositions. Said cosmetic composition is added in an amount, acceptable in cosmetic preparations may comprise an carrier. Wherein, "cosmetic preparations acceptable carrier in" email widow, a web page or cosmetic preparations that can be included in a PAL be already publicly known compound or composition that is used in forward or the compound or composition may be developed are generated when the contact with the skin of the as adaptable abnormal toxicity of, . of the mobile communication network people, Letters and the thing which or irritative instability. Said carrier number skin external composition of the present invention relative to the weight entire cross section thereof about 1 weight % to about 99.99 weight %, preferably about 90 weight % to about weight of the composition, of a body is included to the display apparatus 99.99 weight %. However the ratio said number skin external of the present invention a compositions are made according to the formulation for bar after alcoholic beverage grudge passed it on to an application region of the specific (face, such as neck) a preferred application or its which differ depending on the amount and the like because the, said any ratio whatever side of the present invention involves limiting the range is don't understood. Said carrier as alcohol, oil, surfactant, fatty acid, silicon oil, wetting, moisturizing number, viscous modified number, emulsion, stabilizer, ultraviolet dispersion system, ultraviolet light absorbers, number chromophore, can be the exemplary flavoured. Said alcohol, oil, surfactant, fatty acid, silicon oil, wetting, moisturizing number, viscous modified number, emulsion, stabilizer, ultraviolet dispersion system, ultraviolet light absorbers, number chromophore, fragrances compounds which can be used in already, or the like, that has/composition since the publicly known art if one skilled in the art/composition material information by local and selecting suitable, use can be made of, the. As one embodiment of the present invention, the present invention according to skin external number well the blood plant cells said composition, irritation culture or extract thereof in addition to glycerin, butylene glycol, propylene key roll, polyoxyethylene-hardened castor, ethanol, triethanolamin may include or the like, preservative, [...], colorant, purified water need of may include trace according to. The present invention according to skin external composition number, in various forms may be manufactured, e.g., wash containing a crude drug, seat belt, gel, emulsion, lotion, cream (oil-in-water, water-in-oil bomb, multi-phase), solution, suspension (anhydride and water-based), anhydride product (oil and glycol), gel, mask, pack, powder, gum or gelatin such as a capsule having a coated layer (soft capsule, hard capsule) formulations for example, in such a form can be produced. In the present invention as well as face skin, scalp, systemic is also included a general outline in, which can be applied to such scalp skin external number composition, shampoo, rinse, treatment, or hair tonic, systemic which can be applied to cleanse the body in various forms as and the like can be produced. The present invention according to well the blood plant cells, irritation culture or extract thereof number composition containing skin external the aforementioned manufacturing method are not limited to manufacturing method, the present invention is in the field of the manufacturing method grow having knowledge of typically encountered in making small modifications well the blood plant cells also the present invention according to the method, irritation culture external number composition containing skin or extract thereof can be produced. In particular, the present invention composition is particularly disclosure number said skin external addition a manufacturing method, typically using known manufacturing method, general emulsion formulations and solubilized can be produced in the form of formulations. Skin external composition number according, emulsion cosmetic formulations at nutritional wash containing a crude drug, cream, seat belt is to, solubilized. belonging to dicksoniaceae at cosmetic formulations. Furthermore, dermatological acceptable medium or base by comprising some specific properties, as typically used in dermatological field topical application or systemic an auxiliary can be applied can be produced in the form number. Furthermore, formulations may be in the form of of any cosmetic product suitable, for example solution, gel, solid or dough anhydride product, water dispersed oily to an emulsion, suspension, microemulsion, microcapsules, fine granulocyte or-glucose, an oligosaccharide, a (liposome), form of non-glucose, an oligosaccharide, a sachet dispersion, cream, skin, lotion, powder, ointment, spray or cone thread stick may be provided in form. Furthermore, foam (foam) further contains a propellant or compressed form of aerosol form of composition. may be made from. Furthermore, skin external composition further fat substances and number of the present invention, organic solvent, dissolved number, gelling and number concentrate, softening number, antioxidant number, point that serves as a suspending agent, stabilizer, blowing agent (foaming agent), aromatic, surfactant, water, W emulsion or non-ionic-glucose, an oligosaccharide, a number, charging number, WIPO chelating agent, preservative, vitamin, blockers, damp topic, essential oil, dye, pigment, hydrophilic or lipophilic active agent, lipid vesicles or cosmetic at the any other, such as a component cosmetic diagnostics or dermatological field typically used in can an auxiliary agent. And, the components of the commonly used in dermatological field may for example have been introduced an amount. Such, the present invention according to skin external number convergence composition of the present invention, in a multilamellar vesicle, wound healing, aging and like functional cosmetics include the type of.. In the present invention, when of pharmaceutical compositions, in the embodiment a in a pharmaceutical composition for improving skin condition and can function as. The pharmaceutical composition as an active ingredient, an well the blood plant cells, in addition to or extract thereof culture irritation, "pharmaceutically acceptable carrier" may include, such carrier diluent, [...], coupled number, disintegrating, sweetener, stabilizer; and as antiseptic can be selected from the group consisting of. Said pharmaceutical compositions may further include any additive. Said perfume as an additive, vitamin, and antioxidant can be includes agent. Said pharmaceutically acceptable carrier is capable of providing both backward and carrier, which carrier comprises a which, for example, diluent include lactose-, dextrin, tapioca (tapioca) starch, maize starch, soybean oil, microcrystalline cellulose, or mannitol, [...] include stearic acid magnesium or talc, cellulose hydroxypropyl or polyvinylpyrrolidone having a binder can be. Furthermore, calcium carboxymethyl cellulose include disintegrating, starch glycolic acid sodium, bipolar [...], or a crosstalk gun expense money, sweetener include white sugar, oligosaccharides, sorbitol, or aspartame, stabilizer include carboxymethyl cellulose sodium, Taxus cuspidata, or xanthan gum, jade tentative plan Hyangsan methyl parameters include preservative, jade tentative plan Hyangsan pro it will bloom parameters, or brush Bin may it buys it will be a potassium. Said pharmaceutical composition is publicly known in the art a typical pharmaceutical can be, formulated formulations. Said oral dosage formulations pharmaceutical composition, injection, a suppository, transdermal administration formulations, and a cost and transferring the derivatives, and processes for their preparation of formulated as. can be administered. For example, said formulation liquid, suspension number, masked powders that are to be, granules number, purification, number capsule, bolus, such as-site or off-site can be formulations for oral administration. In another aspect, the present invention refers to including following steps, said ( (A) nucleotide sequence of active well the blood fragment with a extraction the leaves or stem; (B) light fragment with a leaf or stem 0.5-1 mg/L 6-benzyl [...] (6-Benzylaminopurine), L 2/0.3-0.6mg, 4-D, containing life times in MS medium (Murashige and Skoog) to obtain an plant cell cultures well the blood by amount; (C) containing [...] 0.2-0.5 mg/L MS the culture medium to which said (b) step for plant cell cultures well the bloodlife timesnew candle by inducing an amount; and Derived from (d) said (c) step new candle hydroxybutyric of 0.2-0.5 mg/L (containing MS Indole-3-butyric acid (IBA) by amount life times manner in a medium free of the step of inducing root. In the present invention, said (b) external the skin plant cell culture of a robot picks the chip number included in compositions thereby processes of plant cells, culture conditions for life times performs amount is. Specifically, in the present invention following the predetermined value the culture mass for well the blood, (also 2) are capable of proliferating. 1) fragment with a extraction the leaves well the blood used in the present invention under sterile conditions fragment leaves grown a growth in external environment or fragment leaves well the bloodwell the blood leaves external environment, and preferably fragment with a 1 in a growth more than 1000years well the blood fragment with a leaves it is preferable that the using. A extraction fragment with a leaves from well the blood method which is not limited the first substrate are assembled, leaf light using distilled segments onto a surface of fragment leaves removing foreign materials on the recorded in the machining program preferably the function for connecting a terminal used. Stem fragment formed thereon a plurality of holes for. and the contents stored in the database. 2) and sterilization and to Plants grown under sterile conditions well the blood and sterilization and to when the mother pipe having a fragment leaves via but there is no need, in external environment a growth more than 1000years 1 well the blood and sterilization and to the desired program fragment leaves which has undergone the it is preferred that a. A and sterilization and to leaf light in conditions or cancer gun removes a noise fragment with a 70% ethanol to 30 seconds which washes the number sterilization of immersing a sanitizing fluid again (+ Tween20 structure lock 30%) number sterilization after disinfecting ingredient 20 in washed 3 times characterized in that a the sterilizing step. Leaf segments said sterilized distilled water washed with for properly sized to cut a wafer adhered with a plant cells are used in the step of inducing. 3) the step of inducing plant cells Plant from an fragment leaf sterilized step cells are induced to fragment with a leaf sterilized 1 mg/L 6-Benzylaminopurine, 0.3 mg/L 2,4-D, MS basic is included agar 8 g/l and 3% Sucrose medium (Murashige and Skoog 1962, Duchefa yarn, Cat No. M0221) 25 °C in, 70% humidity amount life times conditions on growth of plant cells are induced to initial wherein.. Each media using 1N sodium hydroxide (NaOH) was adjusted to pH 5.8. Performs timely daytime 2 culture passaging. 4), inducing new candle The induced, inducing new candle plant cells or of intermeshing is to multiplate subculture solid media cells produced from the plant seed in gun removes a noise after which consists in, culture temperature but is not limited especially, it is preferred that a 25±2 °C. new candle induction of steps, which media Sucrose 30 g/l the culture medium to which basic MS, adding an Gelite 2.0 g/l, growth regulators material by the addition of 0.2-0.5 mg/L Zeatin includes a adjusted to pH 5.7-5.8 MS 25±2 °C (Murashige & Skoog) and media for culture chamber of 25 °C temperature in main 4 in, 70% humidity on growth of a culture wherein amount life times conditions. Preferably using sodium hydroxide (NaOH) 1N regulated so as to use an pH 5.8. 5) the step of inducing root The derived the step of inducing root multiplate subculture solid media new candle involves culturing the after of intermeshing is to is composed, more specifically such as a cell can plant around new candle off tissue without the need for a frequency signal of a plurality in plant cells divided new middle age several new candle involves culturing the passaging the culture medium to which each is comprised, culture temperature but is not limited especially, it is preferred that a 25±2 °C. Root of the step of inducing Sucrose 30 g/l the culture medium to which basic MS media, adding the n bit parallel data inputted Gelite 2.0 g/l, growth regulators material 0.2-1 mg/L Indole-3-butyric acid (IBA) is added using a 4 weeks in culture chamber of 25±2 °C 25 °C temperature, humidity 70% on growth of guided wherein amount life times conditions. Preferably using sodium hydroxide (NaOH) 1N regulated so as to use an pH 5.8. 6) step well the blood nucleotide sequence of active proliferation well the blood from plant cells for propagating plant derived Root Regeneration and Shoot Regeneration to, Sucrose 10-30 g/l the culture medium to which basic MS, by the addition of of Gelite 2.0 g/l 25±2 °C. 25±2 °C a culture chamber in 4 weeks in culture chamber of 25 °C temperature, humidity 70% on growth of. unit compares wherein amount life times conditions. 7) in differentiated plant with supporting soft handoff a Differentiated in the step for purification of for differentiated in 1:1 is vermiculite and sand and it moves, the core to culture soil herewith to 22-28 °C 60-90% temperature of while as to maintain a relative humidity of a supporting soft handoff difference 1 ; the nozzle is extended from the end, additionally 1 difference temperature of metal after the step of purification of 15-25 °C 60-90% while as to maintain a relative humidity of a supporting soft handoff difference 2 ; may include further. Which has constant temperature and humidity and the optimum culture time through semi-continuous culture plant tissue in an individual with outside environment produced from the plant seed if transferring a poor entity of the communication system, i.e. unable to relate to his environment and apoptosis during tissue culture so that user processing purification of. driving gear group is gear-coupled. 0.5-2g/L degree of salt water can be supporting soft handoff within additional. Furthermore, of the present invention in another aspect, such as on steel or for plant well the blood obtained to characterized by well the blood for sticking optical fibers in sea method relates to transplantation of plant. Transplantation of such plant in terms of method, the art is well known may be included both process is transplantation of plant. Specifically, in addition to the proliferation method step includes: 8) implanting a sea steel or plant For plant well the blood produced from the plant seed, the rod rotates while moving of steel or into the sea by implantation a soil can be is heated. Hereinafter, embodiment to the present invention. as further described further. These embodiment only relate for examples of the present invention, and/or at least two different embodiment of the present invention range interpreted to be limited in the art does not to be will nontrivial twiddle factors and person with skill in the art. In particular, in the embodiment of in hereinafter well the blood plant cells, irritation culture or extract thereof but identifying efficacy is provided, even for itself to the culture not extract and has a semi-permanent function this effect will nontrivial twiddle factors and to one skilled in the art. In the embodiment 1-1 : well the blood from plant cell induced the present invention according to In the present invention used in wide area Incheon it blooms wellthe enterprise only from RIS crab to input part is provided so, well the blood plant with 70% ethanol to 30 seconds which washes the number sterilization of immersing a sanitizing fluid again (+ Tween20 structure lock 30%) sterilization after disinfecting ingredient 20 in number was washed 3 times. Plucked up using sharp knife 1 mg/L 6-Benzylaminopurine in in use, 0.3 mg/L 2,4-D, MS basic is included agar 8 g/l and 3% Sucrose medium (Murashige and Skoog 1962, Duchefa yarn, Cat No. M0221) 25 °C in, 70% humidity on growth of the initial culturing cancer conditions, and induced plant cells. Each media using 1N sodium hydroxide (NaOH) was adjusted to pH 5.8. Timely performed for all the daytime 2 culture passaging. Thus-obtained plant cell culture also look of the result as and 3. In the embodiment 1-2: induction irritation plant well the blood the present invention according to well the blood from plant cells to induce irritation Sucrose 30 g/l the culture medium to which basic MS, adding the n bit parallel data inputted Gelite 2.0 g/l, growth regulators 25±2 °C IBA (indole-3-butyric acid) 0.5 mg/L by the addition of material of good daytime 4 in culture chamber. really end of irritation derived the cut about 1 cm of 1/2 MS the culture medium to which 0.5 mg/L IBA (indole-3-butyric acid), improve efficiency of vaccination liquid culture medium is added is Sucrose 50 g/l was good daytime 4 in culture chamber of. 25±2 °C. Stainless irritation a harvesting medium device and vertically while clean are separated off and the tissue is sufficiently removes moisture of 2 to 60 °C, the third to eo between drying the experiments. Each media using 1N sodium hydroxide (NaOH) was adjusted to pH 5.8. In the embodiment 1-3 : well the blood the present invention according to mass production of irritation plant cell well the blood is induced aseptically from plant cultured well the bloodwell the blood plant cell the for producing a large amount of irritation, as said, when plant cells well the blood, MS the culture medium to which 1 mg/L 6-Benzylaminopurine, 0.3 mg/L 2, the n bit parallel data inputted culture in media containing 4-D, in the case of irritation well the blood, 1/2 MS the culture medium to which IBA (indole-3-butyric acid) 0.5 mg/L, 25±2 °C in media containing Sucrose 50 g/l in culture chamber of, humidity 70% bioreactor having balloon 20L conditions ((main) ternary science) to good daytime 5 to 0.1 vvm air supply amount. Such achieves the mass-production and the look of the also 4 and a equal. In the embodiment 1-4 : well the blood the present invention according to processing number attracting irritation plant cell The present purpose of the invention the well the blood plant cell carotenoid which are the product of secondary metabolic of irritation, biflavonoid and phenolic content of logic state by comparing the method in 1. As physical attractant UV-A fluorescent lamp (20W, Sankyo Denki, Japan) daily for a culture which processing the 0.5h -8h. 2. Chemical attractant MS of shikimic acid 200-600 M as packed in culture the medium (Shikimic acid). Secondary metabolic which are the product of carotenoid, biflavonoid and phenolic content of method for measuring as follows. Total carotenoid content according to AOAC method it was determined that. I.e., freeze-dried acetone in a sample of whole blood irritation plant cell well the blood : nucleic acid (3:7, v/v) extraction by extracting cucumber, a mixed solution prepared then extract obtained filtration and then being washed with distilled water, activated magnesia: diatomite (1:9, v/v) mixtures behind a column chromatography aspiration implanting extract while acetone: nucleic acid (1:9, v/v) mixture in it was determined that in 436 nm absorbance. Sakanaka Colorimetric method based on total flavonoid content such as (2005) method of it was determined that according to. well the blood plant cell irritation culture extract and standard material behind is added is 1.25 ml distilled to 0.25 ml solution, 5%(w/v) NaNO2. 6 has added 0.075 ml solution particular chemical to a mixed solution behind ingredient 10%(w/v) AlCl3 solution after is added is 0.15 ml, then the composition is left for 5 minutes 0.5 ml 1N NaOH solution has added. 2.5 ml final volume space for recalling the mixture is added at a water distilled behind it was determined that absorbance at a wavelength 510 nm. Standard layer is catechin standard solution (+ / -) (Sigma chemical Co. , St. Louis, MO, USA) jet stream by using a carrier gas total flavonoid content mgg-1 DW showed to. Total content well the blood plant cell culture extract irritation Foline-Ciocalteu reagent is total of blue light by molybdenum while reduced by the material which develops a color (Ciocalteu and a Folin, 1927) Foline-Ciocalteu method based on such as Ali (2006a) of total using method it was determined that content. Extract, standards solution behind is added is 0.05 ml distilled to 2.55 ml, 2N Folin-Ciocalteu reagent solution (10 time dilution; Sigma chemical CO. , St. Louis, MO, USA) after minute. 6 has added 0.1 ml 0.5 ml is added is particular chemical to a mixed solution after 20%(w/v) Na2CO3 solution as the dark state to the composition is left for 30 minutes then it was determined that absorbance at a wavelength 760 nm. Standard layer is garlic acid specific gravity of 0.50 to 1.29 g, total content mgg-1 DW showed to. (1) influence of UV-A as physical attractant MS basic the culture medium to which 1 mg/L, with addition of growth regulators Zeatin 0.2 mg/L of the 25 °C culture temperature good daytime 5. Culture with delaying by a main number 6 UV-A fluorescent lamp (20W, Sankyo Denki, Japan) using (hour, time) daily 0h, 0.5h, 1h, was is irradiated with 8h and 4h. (2) influence of shikimic acid as attractant chemical Due to the fact the MS basic the culture medium to which IAA 0.5 mg/L, with addition of growth regulators Zeatin 0.2 mg/L of the 25 °C culture temperature good daytime 5. Number 6 week culturing shikimic acid at a concentration of 200,400 and 600 µm good added to each medium. In the embodiment 1-5 : well the blood plant cell culture for producing the present invention according to Obtained in the in the embodiment 1-3 well the blood plant cell tissue is lower than the harvesting cultures irritation is sufficiently removes moisture of 2 to 60 °C during dryer at was very dry. Dried well the blood plant cell 50g powder culture irritation the support the container, placing of water 1L in 90-120 °C extracted heat time 48. After extraction, mesh are filtered to remove the solids, of an, well the blood to have a filtering for culture extract irritation plant cell, the third to eo to the present invention. Compared the contents address memory, such as extract well the blood and compared extract obtained by extracting several Chinese in method. The present invention according to plant tissue culture techniques well the blood method using nucleotide sequence of active proliferation Said in the embodiment 1 described plant cell induced thereby process, growth on life times amount instead culture, the female conditions and obtaining plant cells, next if there is the specific well the blood was for propagating a plant. 2-1. Induction process (Shoot Regeneration) generating new candlewell the blood from plant cells Obtained in said well the blood from plant cells to induce Shoot Regeneration Sucrose 30 g/l the culture medium to which basic MS, adding the n bit parallel data inputted Gelite 2.0 g/l, [...] material growth regulators (Zeatin) 0.2 mg/L by the addition of good in culture chamber of 252. Being proliferation Shoot Reneration in plant cells, in culture chamber of 252 has a value of a range of temperature in main 4 25,70% humidity on growth of good wherein amount life times conditions. 1N using sodium hydroxide (NaOH) was adjusted to pH 5.8. 2-2. Induction process (Root Regeneration) generating root At the sites where Shoot Regeneration is derived to induce Root Regeneration Sucrose 30 g/l the culture medium to which basic MS, adding the n bit parallel data inputted Gelite 2.0 g/l, growth regulators 25±2 °C Indole-3-butyric acid (IBA) 0.2 mg/L by the addition of material of good in culture chamber. Shoot Regeneration is derived at the sites where one plant nutrients for growing Root Regeneration 25±2 °C of blanks that can be used to for 4 weeks in culture chamber 25 °C temperature, humidity 70% amount life times conditions on growth of. 1N induced wherein using sodium hydroxide (NaOH) was adjusted to pH 5.8. 2-3. well the blood nucleotide sequence of active proliferation process well the blood from plant cells for propagating plant derived Root Regeneration and Shoot Regeneration to, Sucrose 30 g/l the culture medium to which basic MS, by the addition of of Gelite 2.0 g/l 25±2 °C. 25±2 °C a culture chamber in 4 weeks in culture chamber of 25 °C temperature, humidity 70% amount life times conditions on growth of. 4. is heated wherein the implantation into a sea or a steel is after week (also 2). is heated. Experiment 1 e.g.: well the blood (Cytotoxicity) cytotoxicity of culture extract irritation plant cell In the embodiment 1-5 alcohol to the glycolide monomer at skin compositions the to economize in survival rates, human fibroblast (Human Skin Fibroblast, CCD-986Sk) good receiving in the ATCC (American Type Culture Collection). Each cells 1×104 cells/ml on the concentration of was to be inoculated with a 24 well culture plate. Media containing 10% FBS DMEM (Dubelcco ' S Modified Eagle Medium, BRL, USA) by cylinder to have. 10% FBS in DMEM containing 48 time by culturing cultured by 25-30% surface area of the culture vessel, the surface, in the embodiment 1-5 made in FBS-free DMEM containing 0.1-5% composition by replacing a 24 hours' extra good. Culture nor post-incubation 3 - (4,5-aminothiazole dimethyl-2-one) - 2,5-diphenyl tetra thiazolium bromide (MTT, Sigma M5655, USA) solution (2.5 mg/ml) for 50 micro l 3 adding further time to remove the supernatant after grown on, each per well of 200 micro l Dimethylsulfoxide (DMSO, Sigma D2650, USA) solution after loading the formazan formed agitating (formazan) 20 minutes then, determination, 100 micro l of 570 nm to take 96 well it was determined that the absorbance in Enzyme-Linked Immunosorbent Assay (ELISA). Degree of survival rates of skin cells using pure water the control group of absorption strength reference so as to provide expressions as calculated according to the percentage blocked out, tables result as demonstrated 3. [Expressions 1] Proliferation effect cells (%) = [(experiment group absorbance-the control group absorbance)/ collating group of the absorption edge of the] ×100 Using forming cells human keratinocyte said Cytotoxicity be achieved, wherein an influence on results of this test (cytotoxic), table 3 as demonstrated, 0.5%, 1.0% and 5.0% well the blood cell culture extract irritation plant cell toxicity got out of sight. I.e., cell survival rate no influence on. While, according to the concentration processing extract, the well the blood 20-30% have demonstrated cytotoxic rate. Experiment 2 e.g.: well the blood tyrosinase [...] of culture extract irritation plant cell assay (Mushroom Tyrosinase Inhibition Assay) whitening effect Tyrosinase (Tyrosinase) of the ELISA reader (oxidation activity) has been determined to L-DOPA oxidative activation. For purchasing a in the (Mushroom tyrosinase) tyrosinase [...] Sigma-Aldrich l micro. 5 mm L-dopa 10,90 micro l 0.1 M sodium phosphate buffer (pH 6.8), a callus Chinese Quince mixtures of varying concentrations put it in his 96-well plate. Finally 40 for inserting and removing enzyme tyrosinase of micro l after mix, 37 °C 15 minutes in the reaction. The ratio initial of Dopachrome from reactants using extinction 490 nm in ELISA reader at the surface of measure the. Melanin it is sour with the mote but sacrifice L-DOPA tyrosine in a important in the synthesis is switched, waveguide quinone (Dopaquinone). to oxidize L-DOPA to form. Waveguide molten metal the [...]it is sour with the mote but zero been measured (DOPA oxidase activity), test mixtures of varying concentrations by been inhibited by the raw material is placed. [Expressions 2] ×100 Inhibition of Tyrosinase (%) = [1 - (Ab sample/Ab control] Up to 5% well the blood extract, extract and plant cell culture well the blood for tyrosinase inhibitor of culture extract irritation well the blood used as the material for, table 4 as demonstrated, well the blood plant cell culture extract and by culture extract irritation compared to the control group by tyrosinase activity. Experiment 3 e.g.: well the blood plant cell irritation test enhancing synthesis Procollagen of culture extract A human fibroblast (Human Skin Fibroblast) 37 °C, 5% CO2 incubator constant maintaining the humidity of 10% FBS in independently, making various experimental conditions, Penicillin (50U/ml), Streptomycin (50/ml) containing DMEM (Dulbecco 's Modified Eagle' s Medium, Gibco, USA) the culture the, 1x105 cell/ml to 500 micro l then divides the 48 well plate to good time at 24. 0.1-5% concentration of the control group (DMEM medium) and well the blood plant cell irritation culture extract containing a medium for inserting and removing time 48 37 °C, 5% CO2 incubator in good. 48 process has been completed, each the supernatant medium takes an 20 micro l Procollagen Type I C-Peptide EIA Kit (PICP, Takara, Cat No. MK101) by measuring the contact it was determined that amount collagen synthesized de novo. As calculated according to expressions to tables result to the computer of the. shown in an. [Expressions 3] Understructure may be found in said table, of the present invention composition well the blood plant cell irritation culture extract content in the biosynthesis of collagen increases, thereby increasing S70 and a main body, the growth promoting effect collagen synthesis excellent composition of the present invention for a difference from an can be viewed. Experiment 4 e.g.: well the blood plant cell MMP-1 of culture extract irritation and an improvement effect of a small interfering RNA capable of inhibiting expression wrinkles by using Synthesis and a collagen on skin wrinkles and which draft unbalance of degradation usually from the skin collagen type I synthesis and the same enzyme is in an MMP-1. Type I in skin photoaging however, III collagen synthesis is detected before the time, the activity of the MMP-1 for the color temperature.. Such MMPs (matrix metalloproteinases) has a protein source, either extracellular matrix enzyme for decomposing appear to function (extracellular matrix) normal state related to wound healing or tissue regeneration it is also known. MMP-1 composition of the present invention it is confirmed whether an production inhibiting effect, so to, the following paradigm was developed. A human fibroblast (Human Skin Fibroblast) 37 °C, 5% CO2 incubator constant maintaining the humidity of 10% FBS in independently, making various experimental conditions, Penicillin (50U/ml), Streptomycin (50/ml) containing DMEM (Dulbecco 's Modified Eagle' s Medium, Gibco, USA) the culture the, 1×106 then the division 48 well plate to open/ml 24 after grown on time, had a visit from UVB. The control group (DMEM medium) and of concentration of 0.1-5.0% well the blood plant cell irritation cell culture extract 48 time for inserting and removing a broth medium containing rice 37 °C, 5% CO2 incubator in good. 48 process has been completed, each the supernatant medium takes an 20 micro l Matrix Metalloproteinase-1, Biotrack Activity (Amersham, Cat No. RPN2629) measuring the contact a synthesized de novo by then measuring the amount of MMP-1, mg/ml terms, as calculated according to expressions to tables result to the computer of the. shown in an. [Expressions 4] Said table such as 6, the inhibitory effect of the concentration of 0.1-5.0% MMP-1 biosynthesis well the blood plant cell irritation culture extract subjecting the film to a treatment by using the excellent MMP-1 inhibitory effect. In particular, than extract well the bloodwell the blood plant cell by culture extract irritation MMP-1 biosynthesis further film capable of suppressing the occurrence of gene more, exhibiting times. Experiment 5 e.g.: 17alfa respect to inhibition of the active iNOS clause salt effect experiment Raw 264.7 cell line produced from the amount of nitric oxide (NO) present in the cell culture NO2- form by using Griess reagent was in. 1g LPS-activated Raw 264.7 cell to measure of inhibiting the formation NO, said in the embodiment 1 obtained in a concentration of 0.1-5.0% well the blood plant cell with extract of cell culture irritation does multiplier 24 for the control group group the experiments processing after cell culture time the cell culture supernatant Griess reagent 100 micro l and a Griess reagent (1% sulfanilamide in 5% phosphoric acid, 1% -naphthylamide in H2 O) 100l by mixing the reacting a metal salt of 540 nm in 96 well plates in 10 minutes it was determined that absorbance. NO2- the concentration of the sodium nitrate dilution measures the absorbance a standard curve is obtained. Said table such as 7, the activity of the concentration of 0.1-5.0% iNOS well the blood subjecting the film to a treatment by culture extract irritation plant cell compared to the control group NO which inhibits the production, have very good inhibitors of inflammation using the. In particular, well the blood than extract well the blood iNOS is culture extract irritation plant cell of inflammatory activity was further inhibiting effect and water solubility leading to excellent. Experiment 6 e.g.: pore-shrinking effect test (In vitro Test) well the blood plant cell of the present invention composition culture extract irritation for determining effects pore-shrinking said in the embodiment the subject compositions made in the skin replace the protein haemoglobins was test the effects pore-shrinking using. 0.9% Phosphate Buffer Saline (0.1 mm, pH 7.4) in a hemoglobin solution (0.05g/50 ml) said in the embodiment 1 to 2 ml a hemoglobin solution thereby significantly decreasing concentration of glucose well the blood plant cell obtained in a 2 ml culture extract cells irritation after loading the, 30 seconds when blended with an agitated in 3,500 rpm and centrifugation 10 minutes, 2 ml purified water to 1 ml supernatant thereof 407 nm is applied to in factor have been measured by UV-Visible spectrum. Applying PBS (Phosphate Buffer Saline) 2 ml was the control group at. 0.1-0.5% concentration of well the blood plant cell irritation culture extract composition hemoglobin in the composition of a sudden brake, the slider means of a needle electrode of the n bit parallel data inputted estimate the antimicrobial effect shrinkage, (%) precipitation of hemoglobin, contraction of pores high the determined to play effect; a process for manufacturing the. I.e., compared to extract and well the bloodwell the blood plant cell having an excellent effect pore-shrinking is culture extract irritation by. Experiment 7 e.g.: pin experiment inhibitory effect well the blood inhibitory effect of culture extract irritation plant cell skin tones in order to identify a, PC [...] 10 of 4 week or two skin oil measuring instrument (Sebum meter SM810) by sebum secretion of skin using it was determined that amount. 22±2°C experiment, 40±5% humidity in been made. When the oily skin 220 the measured value is micro g/cm2 at least, normal skin when the micro 100-220 g/cm2 is. The n bit parallel data inputted using the control group purified, 9 and a equal tables experiments. From the results of said as seen from table 9, compared to the control group, 1% well the bloodwell the blood is culture extract irritation plant cell by sebum secretion excellent than extract using the inhibitory effect. Experiment 8 e.g.: and an improvement effect of a acne Acne 10 both sexes of the tread is generated for the subject to be name which have face lotion morning, night produced in entire face daytime 4 in the embodiment 1 a 1% concentration of well the blood to bar is used culture extract plant cells. Acne and an improvement effect of a participating experiments determination of identification number, according to a degree of feel with reference to a CDMA a state before test point 1-3 acne healing effect then result to the computer of the evaluate them showed listed in tables so that individual asset. <Test before for evaluation of cancer, cancer > Little negative acne 1 = 2 = 3 = be slightly acne by severe acne <Acne effect determining > - : Effect substantially no, + : some effect sound, ++ : much mitigating, +++ : Completely healing is Understructure is found in 10 of tables, via said, face lotion culture extract plant cells well the blood 1% after it is used in daytime 4 majority subject of acne can be identifying improvement effects of a. Experiment 9 e.g.: ultraviolet irradiation-induced cell protecting effect 302 nm 20 use UV irradiation emitting ultraviolet in a wavelength range of ultraviolet crosslinker CL-1000 by cylinder to have (Ultra-Violet Products, CA). HaCaT cells skin keratinocyte a 1x105 cells/ml 24-well plate in incubator paste has better mouth feeling and concentration grown on time after medium 24 by etching are removed and then the washing buffer phosphate. Phosphate buffer 10 mJ ultraviolet preparing a soil improving agent and a 500 L/cm to which do not contain FBS after irradiation in a cell culture medium replacing a fresh in the embodiment 1 obtained in a well the blood plant cells culture extract, 1% sample culture extract irritation treating with concentration good additional time 24. Herein, MTT (3 - (4,5-dimethylthiazol-2-yl) - 2,5-diphenyltetrazolium bromide, Sigma) 5 mg/ml in incubator after added at a water 3 a are formed after culturing time for fomazan layer covering the both to DMSO, transferred to 96well plate after irradiated with ultraviolet ray measuring absorbance to 595 nm in ELISA reader after processing the specimen with the control group 1 having no and compared rate and cell survival. well the blood plant cell irritation culture extract of keratinocyte protecting effect to assess, human keratinocyte forming cells (Keratinocytes, HaCaT) ultraviolet irradiation 20 mJ/cm 24 then exposed to additional time then, negative photoresist cell survival rate capable of verifying MTT assay result having performed, the culture extract irritation plant cell well the blood 1% -density processing by UV irradiation to the control group compared to cell survival rate (Cell Vialbility) increased. Therefore, well the blood plant cell irritation culture extract V ultraviolet light irradiation-induced cell effect can be identifying. Skin external number for the preparation of well the blood of the present invention plant, irritation culture extract as an active ingredient in cosmetic containing the nutritional wash containing a crude drug, cream, seat belt such as cosmetic and belonging to dicksoniaceae of the dosage form emulsion such as cosmetic formulations such that the solubilization of the manufacturing processes and the cost of production. Manufacturing e.g. 3-1: wash containing a crude drug Then is useful have been prepared according to conventional wash containing a crude drug manufacturing method. Manufacturing e.g. 3-2: seat belt Then seat belt of typically result regimens have been prepared according to manufacturing method. Manufacturing e.g. 3-3: lotion Then lotion typically result regimens have been prepared according to manufacturing method. Manufacturing e.g. 3-4: cream Then approval typically result regimens have been prepared according to manufacturing method. Manufacturing e.g. 3-5: gel Then is useful have been prepared according to conventional gel manufacturing method. At least specific content of the present invention described in detail a portion 360degree, homogeneously distributed to be person with skill in the art, such a preferred embodiment only the specifically and the user makes a is aspect, of the present invention the not range is limited will the apparent. Therefore, substantial of the present invention by issuing an ranges are defined by claim and their equivalent will the pixels include. The present invention relates to an external skin application composition with anti-inflammatory and anti-aging effects, containing a zostera capricorni plant culture extract and a production method thereof. More specifically, the present invention relates to an external skin application with anti-inflammatory and anti-aging effects, containing a plant cell culture material derived from zostera capricorni plant which is a rare plant, an adventitious root culture material or an extract thereof. The present invention further relates to a production method thereof. According to the present invention, the external skin application composition is not toxic to skin cells and exhibits excellent efficacy associated with skin wrinkle amelioration, skin whitening effects, pore tightening effects, sebum secretion inhibition, and acne improvement. COPYRIGHT KIPO 2016 well the blood ( According to Claim 1, for improving skin said to inhibit or wrinkles characterized by skin external composition number accepts improved. According to Claim 1, characterized by said skin for improving skin external number to accepts a whitening composition. According to Claim 1, said skin for improving or ameliorating inflammation of the skin is characterized by skin external composition number. According to Claim 1, said ultraviolet rays to accepts pore-shrinking a skin external composition characterized by number. According to Claim 1, a peer accepts inhibitors to said skin for improving skin external characterized by number composition. According to Claim 1, said inhibit or acne for improving skin is characterized by improved number skin external composition. According to Claim 1, said ultraviolet for improving skin accepts protected the skin by number to characterized by skin external composition. well the blood including following steps ( According to Claim 9, said (a) step, (i) said well the blood are separated from each other and tissue or stem of [...] benzyl 6 - (6-Benzylaminopurine, 6-BAP), 2,4-dichloro phenoxyacetic (2,4-Dichlorophenoxyacetic acid, 2,4-D) containing cellulose in a broth medium by culturing the transformed plant cell cultures to obtain an or, well the blood (ii) said tissue or stem of (IBA) butylic acid indole are separated from each other and containing cellulose in a broth medium by culturing the transformed to obtain an culture irritation, provided that the characterized by method. According to Claim 9, said (a) step in culture, , 3 to 6 time or a UVA, shikimic acid for 150uM-400uM culture added to a broth medium containing rice further including to characterized by method. According to Claim 9, said (b) step, (a) obtained in a well the blood plant cell cultures or irritation of drying and culture, powder mixed water composition producing or, powder is mixed water, ultrasonic extraction or reduces blood cholesterol, platelet including step of preparing the composition to characterized by method. Including following steps, well the blood according to anti number 1 ( well the blood obtained in accordance with Claim 13 for steel or for sticking optical fibers in sea well the blood characterized by nucleotide sequence of active method to implant. UV-A Carotenoid content Flavonoid content Phenol compound content 0h 34 32 45 0.5h 37 37 63 2h 43 36 72 4h 46 37 72 8h 54 38 79 Shikimic acid concentration (µm) Carotenoid content Flavonoid content Phenol compound content 0 43 37 57 100 42 39 67 200 45 36 80 400 46 35 89 600 42 35 62 Processing concentration (%) Cytotoxic rate % (Cytotoxicity) The control group 98 well the blood extraction water 0.5% 82 1.0% 81 5.0% 75 well the blood plant cell culture extraction water 0.5% 100 1.0% 102 5.0% 105 well the skin diligence cultivation extraction water 0.5% 100 1.0% 103 5.0% 108 Processing concentration (%) Tyrosinase Activity (% of control) Purified water (voice the control group) 100 well the blood extraction water 0.5% 97 1.0% 94 5% 90 well the blood plant cell culture extraction water 0.5% 95 1.0% 90 5% 80 well the skin diligence cultivation extraction water 0.5% 95 1.0% 92 5% 83 Processing concentration (%) S70 biosynthesis (% of control) Procollagen The control group 100 well the blood extraction water 0.5% 99 1.0% 105 5% 110 well the blood plant cell culture extraction water 0.5% 108 1.0% 125 5% 130 well the skin diligence cultivation extraction water 0.5% 105 1.0% 115 5% 135 UVB whether processing Processing concentration (%) MMP-1 biosynthesis inhibitors rate (%) - Purified water (voice the control group) 0 + 1% Ascorbic Acid (positive the control group) 30 well the blood extraction water 0.5% 3 1.0% 5 5% 19 well the blood plant cell culture extraction water 0.5% 12 1.0% 20 5% 32 well the skin diligence cultivation extraction water 0.5% 8 1.0% 15 5% 30 LPS whether processing In material Concentration (%) Cell Viability (%) NO Production (M) - The control group (purified water) 100 1.3 + The control group (purified water) 760 3.6 well the blood extraction water 0.5 99 3.6 1.0 100 3.5 5.0 101 3.4 well blood plant cell times amount extract 0.5 101 3.4 1.0 100 3.3 5.0 101 3.2 well the skin diligence boat amount extract 0.5 100 3.3 1.0 102 3.2 5.0 105 3.1 Indomethacin 100M 95.8 3.1 (%) Also of agricultural (%) Precipitation of hemoglobin PBS (voice the control group) 0 0.1 Tannic acid (positive the control group) 1 35 well the blood extract 0.5 1 1.0 3 5.0 5 well the blood plant cells culture extract 0.5 10 1.0 17 5.0 30 well the skin diligence cultivation extraction water 0.5 5 1.0 20 5.0 33 In material Pin inhibitory effect 0 (before use) 28 (after use) The control group 230 225 1 well the blood extract 1% 231 195 2 1% well the blood plant cells culture extract 230 171 3 1% well the blood irritation culture extract 232 165 Subject Test-conducting state 2 main device after a lapse effect 4 main device after a lapse effect Subject 1 2 + + Subject 2 1 + ++ Subject 3 1 - + Subject 4 2 - - Subject 5 2 + ++ Subject 6 2 + + 7 subject 3 ++ +++ Subject 8 2 - ++ 9 subject 3 + + 10 subject 2 + ++ Whether UV irradiation Concentration processing Ultraviolet irradiation-induced cell protecting effect (% of Control) UV (-) The control group - 1.0 UV (+) The control group - 0.5 1% well the blood extraction water 0.52 well the blood plant cell culture extraction water 0.72 well the skin diligence cultivation extraction water 0.81 Won material name Weight % (w/w) Glycerin 5.0 Dipropyleneglycol methyletheracetate and 3.0 Anionic 0.5 Polyoxyethylene-hardened castor 0.1 one ethyl oleate polyethylene 0.1 Ethanol 5.0 Preservative 0.15 [...] Dosage Colorant Dosage well the blood plant, irritation culture extract 2.0 Purified water To 100 Won material name Weight % (w/w) [...] diacetonitriles 1.0 Self-mono-stearic acid 1.0 Lycopene 0.5 [...] 5.0 [...] isocyanate 3.0 Polydimethylsiloxane 0.3 Sorbitan stearic acid mono 0.5 Polyethylene glycol stearic acid mono 8.0 Glycerin 4.0 Propylene glycol 0.2 Carboxylic polymer 0.22 Triethanolamin 0.25 Preservative Dosage Perfume Dosage Colorant Dosage well the blood plant, irritation culture extract 7.0 Purified water To 100 Won [...] Weight % (w/w) [...] diacetonitriles 0.8 Self-mono-stearic acid 1.0 Lycopene 0.5 Stearic acid 0.5 Flow paraffin 7.0 [...] 5.0 e car missing child oil 3.0 [...] isocyanate 2.0 Polydimethylsiloxane 0.3 Sorbitan stearic acid mono 0.5 Polyethylene glycol stearic acid mono 1.2 Glycerin 4.0 Propylene glycol 4.0 Betaine 4.0 Carboxylic polymer 0.12 Triethanolamin 0.15 Preservative 0.25 Perfume Dosage Colorant Dosage well the blood plant, irritation culture extract 5.0 Purified water To 100 Won material name Weight % (w/w) [...] diacetonitriles 3.0 Self-mono-stearic acid 1.5 Friendly type mono-stearic acid 1.5 Lycopene 0.5 Flow paraffin 8.0 [...] 7.0 [...] isocyanate 4.0 hu good type purification 4.0 Polydimethylsiloxane 0.3 Sorbitan stearic acid mono 1.0 Polyethylene glycol stearic acid mono 1.2 Glycerin 6.0 Propylene glycol 4.0 Betaine 4.0 Ammunition gum 0.06 Triethanolamin 0.10 Preservative 0.25 Perfume Dosage Colorant Dosage well the blood plant, irritation culture extract 7.0 Purified water To 100 Won [...] Weight % (w/w) Glycerin 4.0 Propylene glycol 4.0 Ethanol 10 Polyoxyethylene-hardened castor 0.1 Carboxylic polymer 0.30 Triethanolamin 0.30 Preservative Dosage Perfume Dosage Colorant Dosage well the blood plant, irritation culture extract 1.0 Purified water To 100
