COMPOSITION FOR PREVENTING OR TREATING LIVESTOCK DIARRHEA, COMPRISING OSTHOLE AS ACTIVE INGREDIENT

Hereinafter, the present invention more rapidly and to reduce a memory..

Of the present invention according to one aspect, the present invention refers to diseases which contain as the active ingredient of chaos wahing [...] for preventing or treating diarrhea in cattle is directed to compositions.

In the present invention, said natural products which is one of chaos wahing [...]phosphorus system coumarin, in in decimated but mainly casualties (Cnidium monnieri), some other plants can be extracted even.. [...] of chaoas type in formula 7-methoxy-8 - (3-methyl-2- neel view reel ) coumarin (7-Methoxy-8- (3-methyl-2-butenyl) coumarin) or 7-methoxy-8- pen mote Neel coumarin isocyanate (7-Methoxy-8-isopentenylcoumarin) is represented by. The toxic effect at concentrations by [...] of chaos wahing said't is (also 1).

One embodiment according to the example of the present invention, said a γ-IFN [...] of chaoas type is suppressed.

A γ-said IFN a dissolvable configured as a dimer as cytokines, congenital. important for activating immune and adaptive. Macrophage migration inhibitory main γ-said IFN an active agent and major histocompatibility complex class II is derivatives. Excessive IFN-γ self releasing hgrf to the outside of cells relates to inflammatory and autoimmune diseases are known as HMG-COa reductase.

TNF-α and γ-IFN [...] of chaos wahing said inhibit secretion (also 2 and 3). Subjecting the film to a treatment by a LPS TNF-α B cells are stimulated and the secreted (B lymphocyte) and, subjecting the film to a treatment by a γ-said IFN Con-A T cells are stimulated and the (T lymphocyte) for being stored material that has. In said gene expression levels of chaos wahing [...] TNF-α and γ-said IFN such as cytokine production than the size of the gate hole to restrain generation of a protein to it was observed that (also 4 and 5).

One embodiment according to the example of the present invention, said diarrhea bovine Rotavirus, bovine corona virus, e. coli, Salmonella and Pasteurella (Pasteurella) 1 selected from the group comprising at least one can be caused by. However limited to not.

Using immatured said bovine Rotavirus virus in a bad calf Young within main 2 causing the excrements which it will bite even if as virus, infectious WIPO. Coordinators 1 said bovine corona virus in S1 and calf of coordinators months 3 in causes the diarrhea, latency is 7 or more using immatured usually short and to 1 degree of main 4-6 calf in a bundle. Said Salmonella bacteria [...] to human or animal body, as well causing the extraction of the diseases, causing food poisoning is a. Bacteria (Pasteurella) Pasteurella said upper airway of animal and birds mammal agent to the mucous membranes of the digestive tract and a parasitic grown on agar the blood is connected to the semiconductor layer. that.

One embodiment according to the example of the present invention, said livestock characterized in that the calf.

Said calf representative by the apoptosis in case there occurs a diarrhea, immunization is atrophy tissue thymus gland concerning cytokines in leukocyte in blood inflammation induced protein secretion (Cytokine) that. are opened upward and downward. The respiratory immune thereby extending into the hollow interior for permitting collapse force incidence is increased and dehydrating and fell down eventually a bushing appearing deficiency and decreased bone physical strength is dead. The intestinal from the mucosa [...] of chaos wahing said calf said excessive inhibiting the secretion of cytokines by calf said reduces the locates between the division due to diarrhea.

One embodiment according to the example of the present invention, 100 parts by weight of said composition, said 0.3-1.0 [...] of chaoas type can be parts by weight of.

One embodiment according to the example of the present invention, said a carrier composition, number excipient, may further include any or diluents.

Said pattern is removed very small amount of carrier, similar wise added to for extracting with material and chemistries, said excipient suitable the medicament and MnO2, and the frequency or shape allows for quicker, or subject having a small amount of decides capacity, the weight to a size which is easy to handle added material that has the purpose. Said null dose excipient which, without disrupting the therapeutic effect, prevent troubles from occurring test's just not on. Solid suitable excipients, diluent or liquid the solidus surface which it gives, charging number and all types of formulations as FRP pump, a preferred formulations may be in the form of purification, coated tablet, capsule number, bolus, granules comprises such as number. The above adsorbent to raw material trace a particular diluent said or varied without varying the physical properties thereof added to lower the concentration of the as a, include a diluent that is of the commonly used calcium phosphate, kaolin, grinding plaque minute, salt, sodium sulfate, sugar, soybean ingredient, but may include fibers, maltose, are not limited to.

By another aspect of the present invention, said composition characterized by including to feed additive or antibiotic replacement agent may be provided.

The present invention a composition by delivery of the oral, rectal or intravenous, muscle, avoid, intrauterine epidural (intracerebroventricular) in cerebrovascular or can be as administration by injection. 110-100 ml extract of the present invention, preferably 20-80 ml, more preferably 25-70 ml preferably administering an. However said dose constitutes a not limited to and, in cattle can be adjustable state.

Hereinafter to embodiment in the present invention. as further described further. Just, these embodiment only relate is a section for examples of the present invention, and/or at least two different embodiment of the present invention range not be interpreted to be limited will.

< 실시예 1>Korean tradition oxen peripheral blood immune of separating and of chaos wahing toll effect experiment

Korean tradition oxen blood blood collection 50 ml EDTA 5 ml -500 mm per in-site samples is added after transported to laboratory using leucosep tube and a ficoll-paque immune cells after the separation of the toll of chaoas type, we have demonstrated that despite effect of (American Sigma).

Experiment 1e.g.: immune cell separation

The order to proceed with experiment, of the following listed in tables so that individual asset substrate reagents and centrifugal separator, CO₂ incubator, 37 °C constant temperature water tank, and clean bench and Real-time PCR by using instruments such as.

Calf blood which is collected from PBS dilution ratio 1:3 using the 4 times after forming, blood call 80G Leucosep tube (Ficoll) for 1 minutes in 3 ml for inserting and removing of the tube centrifugation moves underneath the barrier (barrier). Next, 8 ml blood dilution Leucosep tube for inserting and removing 1800G to 10 minutes at room temperature after centrifugal separation, and separation layer of a on barrier layer after removing contaminants and metal oxides in solution from above new separation layer was to shorten a manufacturing time and to tube. Displaced in a separating layer 5 minutes in PBS 80G using centrifugal separation, and then I clearly by washing 3 times, finally DMSO 10% using media with includes visitor is checked through a suspension of cells. After counting cell suspended 1 X 10⁷ cell/ml was freeze at frequency divides the tube. Heating and hot-storage time in -20 ° C 2-3 transferred to refrigerator of -80 ° C during night after putting a was the control unit in liquid nitrogen.

Experiment 2e.g.: reagent processing

The order to proceed with experiment, of the following listed in tables so that individual asset substrate reagents and centrifugal separator, -20 ° C freezer, refrigerator, LN2 tank, water tank shaft insert injection molding double body butterfly 37 °C, clean bench and freezing by using instruments such as tube.

Cryopreservation in tank systems of 37 °C freeze filled with cells then thawing rapidly tube into the, both cells freeze tube 15 ml 80G collected in upper layer centrifugal separator 5 minutes, pouring out, new RPMI 1640 medium for inserting and removing he suspension cells. After counting cells suspended 1 X 10⁶ cells/well (well) was 24 at frequency divides the well. 2 time for dividing cells after LPS and a stabilizing and then after each handling a Con-A [...] of chaoas type concentration each step 72 . 72 good time culture nor post-incubation time the culture a culture cells and stored in a -20 ° C, are extracted RNA from cells and super SCRIPT II reverse transcriptase kit used as the combined into the cDNA. The cDNA synthesized TNF-α and Quantitative Real-Time PCR (qRT-PCR) IFN-γ of primer using the gene was and quantitatively relative expression.

Experiment 3e.g.: ELISAassay

The order to proceed with experiment, of the following listed in tables so that individual asset Squeeze bottle and materials described by using instruments such as spectrophotometer and.

Standard continuously prepared in by the respective dilution after concentration, 100 μ l and the sample standard plate Assay put each well. Cover film is formed on the substrate to cover after grown on time 1 at room temperature, wash buffer wash up water burn 4 plate in a (washing buffer). Each well (Detection antibody working solution) 100 μ l solution working body anti detection for inserting and removing the cover film then grown on time after 1 at room temperature, wash buffer wash up water burn 4 plate. Each well (Streptavidin-HPR working solution) 100 μ l [...] -HPR working solution for inserting and removing the cover film then grown on after 30 minutes at room temperature, wash buffer wash up water burn 4 plate. Furthermore, each well substrate solution (TMB Substrate solution) 100 μ l TMB darkroom for inserting and removing grown on in a high temperature to the normal temperature after 30 minutes, each well stop solution (Stop solution) 100 μ l capable of stopping the reaction agent is placed in spectrophotometer after it was determined that wavelength 450 nm absorbance.

Experiments

Korean tradition oxen peripheral blood of immune cell inflammation in immunization antigen substance which causes a LPS (immunogenic of macrophages and lymph B) and Con-A ( lymph immunogenic T) for processing after the mobile phone confirms the effect of chaos wahing [...] result, toxicity in a concentration by [...] of chaoas type identifying improving had no effect (also reference 1). Furthermore, concentration dependent fashion that give rise to an immune reaction-inflammation in γ-IFN and TNF-α by inhibiting the secretion of immune modulating inflammatory allitol of chaoas type verify that the a material can be (also 2 and 3 reference).

Gene expression of these proteins in skin tones in order to identify a effect of chaos wahing [...] qRT-PCR performing a result, gene expression levels of chaos wahing [...] independent the concentration identifying improving has inhibition effect. Furthermore, with respect to such TNF-α and IFN-γ allitol of chaoas type inflammatory cytokine production inhibitors in gene expression levels by for producing proteins inserted has been confirmed (also 4 and 5 reference).

The present invention at least described in detail a portion specific content 360degree, homogeneously distributed to be person with skill in the art, such a preferred embodiment only the specifically and the user makes a is aspect, of the present invention the not range is limited will the apparent. Thus of the present invention by issuing an ranges are substantial claim and their equivalent defined by will the pixels include.