DRUG DELIVERY SYSTEM COMPRISING GELATIN NANOPARTICLES SLOWLY RELEASING POORLY SOLUBLE DRUG AND METHOD FOR PREPARING SAME

The present invention refers to a sparingly against the water when it is moved, to easily absorb it are soluble and drug microscopic particles of the display panel in which to bring an homogenizer (homogenizer) without nanoemulsion artworking method similarly (or nano suspension) W/O or O/W/O by a rope. system to be constructed. Wherein the frequency W/O and O/W/O/sparingly soluble drug/fatty acid/fatty acid nanoparticles gelatin and gelatin nanoparticles. correspondence with a migrate. In the present invention gelatin nanoparticles patient as solvents for the clearing unit number (W) of a solvent dissolving the in 40-60 °C gelatin, a hardly soluble drug in a addition or without added. under trillion number the solution. The sparingly soluble drug paclitaxel, coenzyme [...], the right base cow jade hour choline it buys, [...], or IMATINIB mesylate patient includes one or more than two kinds among (W) of solvent includes DMSO. Fatty acid in mixture of one or more, addition or a hardly soluble drug in said-free gelatin aqueous solution or supports the addition of dribbled drop of to 200 nm nanoparticles gelatin. under trillion number. Adding a number surfactant the fatty acid can be the number said surfactant sorbitan monoisostearate, sorbitan monooleate, sorbitan sesquioleate or sorbitan trioleate of sorbitan includes system of an exposing apparatus is replaced. Surfactant number added to the fatty acid is a hardly soluble drug in a gelatin metal added to the nanoemulsion system O/W/O, and if the data (or suspension) is generated on. Wherein/sparingly soluble drug system O/W/O gelatin nanoparticles. correspondence with a migrate/fatty acid. Wherein gelatin nanoparticles free from immune system since the circulation time within human nanoparticles gelatin [...], extending relatively than the particles. The Fatty acid such as oleic acid or linoleic acid kinds of fatty acid or various kinds of fatty acid, in addition to mixtures which, at room temperature the engine thereby for reducing fuel consumption and fluid, - in the fire extinguisher in body Bifidobacterium and for circuit controlling isolation gates of proliferation cancer cells by bacterial strains having anticancer activity and number conjugated (conjugated) input oral is linoleic acid converted into linoleic acid (oral administration), when considered in the to is preferred. In addition linoleic acid in the skin per day to benefit properties which consists of cosmetic industry, when applying to the skin an an antiinflammatory, acne reduction and moisturizing.. The present invention is of the existing method O/W/O or W/O emulsion system and the homogenizer where otherwise a without using a (homogenizer), patient (W) of gelatin solution that the solvent is oil phase (O) of the gelatin particles are spread fatty acid is nanosized formed gel (gel) is sub-a copyright 2000. Gelatin solution external grudge number water solvent DMSO as polar solvent includes or the like.

Gelatin complex as adult an amphipathic having molecular structures, an Azithromycin writing, hydroxy [...][...] hydrophilic with an amino acid such as tryptophan, tyrosine, alanine, [...] scene, idle [...][...] contained simultaneously, amino acids such as of wet liquid to flow down. Thus created are gelatin nanoparticles on fatty acid and, at the same time, amphiphilic, surfactant number gelatin in surrounded by hydrophilic segment of stability of nanoparticles can be for high number, hydrophilic gelatin surrounded branched chain surfactant number of hydrophilic segment is contacting. The gelatin nanoparticles sparingly soluble drug in a solvent to form a slurry within the gelatin nanoparticles chains [...] of gelatin and a second step is that, upon release of drug the sparingly soluble drug in a solvent to form a slurry from the interior gelatin nanoparticles outside gelatin nanoparticles emission sustained release can be is (slow release). The invention refers to the against the water when it is moved a sparingly soluble of the substance is gelatin nanoparticle surfaces and a rather than the to locate the nanoparticles supported. for high number for sustained release of drug.

Surfactant number to 1 volume specific non-solvent process input of gum or gelatin less of the total combined weight 30 to 35 times the 2 w/v % surface-active number a, gelatin before for producing nanoparticles fatty acid to 1 after it has been deposited by either adding gelatin for producing nanoparticles within a time is is introduced the fatty acid. Surfactant number even when the dispenser is not in use is generated and gelatin nanoparticles gelatin nanoparticles (opposite charges) opposite between such charge (like charges) non-dropped by but stability-resistant, gelatin nanoparticles to do not dissolve in in water for cross-linking the gelatin within particles crosslinked gelatin when enable the application number Massing of an between nanoparticles is stable to prevent raveling and number as enable the application KIPO & number surfactant. Crosslinked crosslinked natural as number (genipin) [...] number in number, glutaldehyde (gluta-aldehyde) or glyoxal is provided, includes (Glyoxal). Generated gelatin nanoparticles dissolved gelatin and fatty acid used to number and second fitting unit is separated from the solvent, solvent dissolved gelatin and gelatin nanoparticles and fatty acid using a density difference between the a centrifugal separator, a fatty acid to triggers number polar or nonpolar solvent added winding or performing provided to simplify the dispersion can be repeated, with the. Here polar solvent ethanol, methanol and ether of number for includes a non-polar solvent with toluene, carbon tetrachloride, benzene and xylenes for such as includes a number. Then are redispersable in gelatin nanoparticles or followed by freeze-drying, using a membrane dialysis and drying, gelatin nanoparticles is re-and which do not dissolve in 40 °C evaporation and vacuum at a temperature of less than its equivalent gelatin nanoparticles the upper electrode layer, physically and chemically in process number and separation, the process or, is 10 to - 40 processes such as a plurality other gelatin nanoparticles performed to expose process can be under trillion number.

In addition sparingly soluble drug nanoparticles gelatin is carried, forward solvent mixture dissolved gelatin and a fatty acid number without revealing the/or isolating a proper system for a self mixture, or mixtures thereof with a water-soluble of emulsifying the oral number material (oral administration) to the lateral center part of the skin cancer can be percutaneous absorption, by applying to the target skin cancer as anticancer number, use can be made of, .

In hereinafter, against the water when it is moved a sparingly soluble drug, gelatin nanoparticles containing specific for bath number a is in number in the embodiment.

<>Paclitaxel nano particles in the embodiment 1 carried inside of a gelatin bath number of nanoparticles

Gelatin 40 mg for 2 ml of DMSO to dissolving the while maintaining the 60 °C, obtain a lead line having a paclitaxel 0 solution. 5 mg has added. Surfactant number as sorbitan sesquioleate 1. 30 ml of a 5 ml linoleic acid then a step of preparing a solution by added to, gelatin solution added with Paclitaxel linoleic acid was liquid is dropped on the solution. 15 minutes after crosslinked 5% added 96uL [...] number as, for particles in a crosslinkable nano gelatin generated about 12 1000 rpm time is set in agitating section. Containing nano particles and having high strength gelatin generated the 12000 g to centrifuge 15 minutes using a supernatant in linoleic acid centrifugal after separation was authored in the first client by a number. Gelatin nanoparticles separated 6 ml of ethanol after adding vortex in a distributed environment with , the aforementioned centrifugal work during the address number that triggers a supernatant 2 iterations performed for all the process. After the gelatin nano-particle is 3 ml distilled after the redistribution by adding -75 °C pre to using freezing dryer and was very dry liver 2. Gelatin generated while a solution containing nanoparticles (Malvern yarn zetarsize Nano ZS) bindings particle size for the gelatin using other potential it was determined that number and nano particle collector. As a result about 200 nm, very uniform being, gelatin nano particle generation been found.

<>Paclitaxel nano particles in the embodiment 2 carried inside of a gelatin of nanoparticles drug release experiment

In the embodiment 1, a pore-forming matter number by applying the number of a paclitaxel under trillion nano particles carried inside of a gelatin the nanoparticles are utilized to experiment is performed for all the drug release. Three multi function cap respectively PBS (pH 7. 4) a 50 ml paclitaxel prepared by the method number with the filling and each containing 10 mg - gelatin nanoparticles using cultivation it will raise freely 37 °C by the addition of 100 rpm in succussion occurs section. Multi function cap to 24 hours after succussion has added trypsin 25 mg each. Succussion occurs after 10, 20, 30 ingredient, 10, 20, 30 ingredient after 1 time and 1, 2, 3, 4, 5, 6, 12, 24, 25, 26, 27, 30, 33, 45, 51, 69, 81, 94, 100 each sample to third time as blocks, 205 nm 230 nm (Paclitaxel) and each at a wavelength of (Linoleic acid + Paclitaxel) each following to measurement absorbance was recharging a multi function cap to. Multi function cap each time in a wavelength of absorbance progression according to 230 nm as Figure 1. Succussion occurs after the n bit parallel data inputted is affectable until time environment including the 6 12 time using the increased absorbance some beyond. However 12 24 time has passed from the introduction of time that experiences a larger absorbance increase was shown a tendency of rising. Trypsin is added is is affectable while from decomposition nanoparticles gelatin after 29 and 30 until after time until time 66 from time abruptly increases in absorbance increase from the introduction of the to 100 volts until time extinction 100, which has the normal state. Thus succussion occurs after 24 time 28 of paclitaxel nanoparticles gelatin in a solvent to form a slurry. 6% been released.

While paclitaxel encapsulation efficiency for the particles nano gelatin of the actual loading (0. 1 mg PTX/10 mg NPs) for theoretical loading (0. 5 mg/(0. 5 mg + 40 mg)) 80 divided by as. This solution is poured into a 4%. Gelatin nanoparticles amount and gelatine from paclitaxel nanoparticles in a solvent to form a slurry that purified bath number linoleic acid and ethanol supernatant included in each the amount paclitaxel 0. 0 and 1 mg. This solution is poured into a tender 106 mg. The remaining would not be thus the amount paclitaxel 0. 294 mg as gelatin solution amount of paclitaxel a and to fix an arm 58. 8% (0. 294 mg/0. 5 mg) is. This cut-off centrifugal separator particle size than the pellet through separation of centrifugal small the seed not gelatin nanoparticles is estimation amount paclitaxel in a solvent to form a slurry. 20% yield is supported in addition paclitaxel (0. 1 mg/0. 5 mg) as a gelatin nanoparticles yield 37. 5% (15 mg/40 mg) than low (table 1).

The storing, centrifugal separator centrifugal separation conditions that time and density tea and the like cut-off of particles which are separated from continuously size in product muscle proportional the number viscosity phase fluid. Viscosity of the water in continuous linoleic acid 20 °C the viscosity of about 20. 7 back size cut-off as about 4. 8 back 2000.

Thus gelatin nanoparticles when ethanol or a by water different continuous yield the free surfaces with far less computing power than was.

205 nm while extinction progression according to time at a wavelength of Figure 2 and a area as Paclitaxel and a Linoleic acid exhibits a sum of. Trend of Paclitaxel indicative of the in Figure 1 after shaken while visible absorbance some until time 12, in Figure 2 similar 3 - 4 together release and quickly during time from the introduction of time have demonstrated behavior in steady state until time 12. This gelatin nanoparticles external the suction hole is established near the very small amount of linoleic acid is emitted by quickly displacing the PBS is shown to, after shaken Linoleic acid 12 should the tank supply become depleted, a adsorption process has been completed after activation by a user which is behavior of as a result, the center line of Figure 1 Paclitaxel behavior. Thus gelatin nanoparticles external the suction hole is established near the very small amount of linoleic acid is gelatin nanoparticles internal in a solvent to form a slurry and for circuit controlling isolation gates of time about release of paclitaxel while number 12, gelatin nanoparticles inside outside nanoparticles gelatinizes paclitaxel was sustained release.