NOVEL LACTOBACILLUS CASEI DK128 STRAIN HAVING PROBIOIC ACTIVITY AND METHOD FOR PRODUCING OPUNTIA HUMIFUSA-FERMENTED MILK FOR IMMUNIZATION REINFORCEMENT OF ANIMALS USING SAME

The purpose of the invention to achieve, the present invention refers to the probiotic Lactobacillus car number (probiotic) activity (Lactobacillus Casei) DK128 strain number [...] substrate.

The antiallergic agent of the present invention said car number (Lactobacillus Casei) DK128 acid resistant strains, private talk juice acidic, resolution and an hundred qualities, it is cauchy writing base number β - β - galactosidase persimmons having excellent performance and secretion when disclosed number secretion, probiotic activity identified persimmons separated from traditional foods.

In addition, the present invention refers to said strain, the of the handle, said handle including a buffering composition number number [...] immunity-enhancing animal milk concentrate or dry matter ultra [...] substrate.

In one embodiment of the present invention according to at least two, said antioxidant functionality may be employed, can be a antimicrobial having activity of force f is increased, the number is not one.

In addition, in the present invention said Lactobacillus car number is (Lactobacillus Casei) For processing a crude or removal of fat gushing oil persimmons [...] DK128 adding fermented animal milk [...] seconds number search for the high pressure liquid coolant immunity-enhancing physiological activity, antioxidant capability, and methods employed for antimicrobial activity conducts an experiment result, of the present invention is Lactobacillus car number (Lactobacillus Casei) Of the present invention is the non-milk fermented [...] seconds persimmons DK128 persimmons [...] seconds crude or with the reserved disclosed antioxidant employed for making fermentation, significantly excellent antibacterial having activity of capacity has been confirmed.

The, of the present invention is Lactobacillus car number (Lactobacillus Casei) DK128 strain, of culture, said handle including a milk concentrate or dry matter animal immunity-enhancing [...] seconds number milk composition employed for quiet antioxidant [...] seconds, such that the antimicrobial activity of substantially elevated immune ability, milk can be useful for developing animal immunity-enhancing [...] seconds.

Lactobacillus car number is said (Lactobacillus Casei) Culture of Lactobacillus strain is Lactobacillus strain is the strain number DK128 DK128 DK128 car number car would the inclusion of various materials in animal immunity-enhancing composition number [...] seconds when quiet milk ingredient, comprising said compositions can exhibit the same effect strain of a lactic bacterium.

Of the present invention said composition further comprises a pharmaceutical carrier and excipient effective in addition number can be conventional, these compositions contain conventional manufacturing method is ten dry or lyophilized probiotic composition according - locus number by using high pressure liquid coolant can be drying form number. Relative to the weight of the total composition of the present invention composition, the active ingredient 10 DK128 strain Lactobacillus car number⁵ To 10¹² Cfu/g, preferably 10⁷ To 10¹¹ Cfu/g can be of content, each of the culture of the strain Lactobacillus car number DK128 SDA 10 active ingredient⁶ To 10¹² Ml/g, preferably 10⁷ To 10¹¹ Ml/g can be of content.

In addition, the present invention refers to said strain, of culture, said handle concentrate or dry matter added to the crude or in removal of fat gushing oil [...] seconds to achieve an animal said method and manufacturing method including milk [...] immunity-enhancing immunity-enhancing animal prepared by the number by a number milk seconds seconds [...][...] substrate.

In the present invention, said strain method of contrast can be incubation method typically used a and fermentation.

In addition, the present invention refers to said animal milk as an active ingredient [...] seconds immunity-enhancing feed additive composition number number [...] substrate.

Strains such as described above and said order, a feed additive number immune enhancing composition can be added disclosed. Number feed additive of the present invention is therefore on feed quality control method corresponding to the substrate.

Number feed additive of the present invention composition can be added to feed compositions, animal "feed" is reduced, and relieves, or extinguishing any suitable natural or artificial diet, such as meal or meal component can be said means. One kind of non-feed said specially number correspondingly, normally used in the art with a feed can be used. Examples of said feed but number, manufacture, with muscle type, food processing current, avian, fibrous current, number about by current, oil-and-fat, before classification, such as [...] or grain products like vegetable feed; protein current, inorganic current, oil-and-fat, mineral flow, oil-and-fat, or single protein, such as animal feed or a food animal substances is cited.

Hereinafter, the present invention in the embodiment by a detailed as follows. Stage, in the embodiment for the present invention is exemplified ephemeral is, in the embodiment of the present invention to not the limited to contents.

In the embodiment 1. Immunity-enhancing animal [...] seconds For milk number numerical control machine Fermentation strain Selecting

1 - 1. Lactic acid bacteria fermented animal immunity-enhancing ability selecting excellent [...] seconds

Korean traditional foods after separating container 60 species based on identifying physiologically active verifies derived from lactic acid, a lactic acid HCl for 1 to table 1 difference moieties 36 two experiments using water, bile salts (bile salt) experiments using bile acid resistance, when disclosed number β - galactosidase protein degradation vitality and active, s [khwul phosphorus agar test (Esculin agar test) embodiment experiments was selecting 8 lactic acid species.

1 - 2. Korean traditional foods physiologically active identification and 2 difference selecting derived from lactic acid bacteria

1 - 2 - 1. Korean traditional foods derived from acid resistant bacteria

Identifying lactic acid resistant of separating container for measuring pH 2. 0 2 In MRS broth culture time after time and 24 adjusted to DK101, DK102, DK104, DK107, DK109, DK119, DK121, DK128, DK207, DK211, DK212, DK215, DK301, lactic acid bacteria culture is about the same time levels in total 14 species DK303 maintains high survival force 0 rotary into foods.

1 - 2 - 2. Korean traditional foods lactic acid bacterium derived from bile acid resistance

For measuring bile acid resistance of lactic acid 1. 0% Oxgall 37 °C MTS broth culture nor post-added in 2 confirmed viability in time. A range of tolerance DK128 lactic acid, DK207, including paper DK DK212 DK211 and total 32 10 strains⁷ (CFU/mL) representing more viability has been confirmed.

1 - 2 - 3. Lactic acid esters derived from Korean traditional foods it is cauchy writing base number vitality through β - [khwul phosphorus agar test (Esculin agar test)

It is cauchy writing base moieties per of lactic acid utilized as indicator number in order to identify activity using ability such as β - 1 [khwul phosphorus agar test Agar well diffusion method was also used as a testing method. The test result s [khwul phosphorus agar plate formed in bacteria around of the resulting transparent housing DK109, DK119, DK121, DK128, DK207, DK211, it is cauchy writing base cream (also 1) of β - DK215 DK212 and identifying number vitality, it is cauchy writing base number vitality was very insignificantly other strain is β - free. The number remaining strains is insignificantly [...] DK strains on according to the sugar using capability, low capacity [...] seconds fermentation has been expected.

1 - 2 - 4. Hundred qualities as to Korean traditional foods derived from lactic acid decomposition vitality

Selecting hundred qualities as to lactic acid was used as a test for measuring resolution Well diffusion method. Formed in bacteria mixture comprises the cap around its generated on ring moieties reporting activity has been confirmed that the hundred qualities resolution as to lactic acid.

DK119 determination result, and DK128 hundred qualities have been found as to DK121 3 inhibitors for the treatment time from the time of culturing DK109 ring 72 was identified, other DK strains [...] hundred qualities as to number remaining strains other resolution is low compared differentiation strains as signal peptides.

1 - 2 - 5. Number disclosed in Korean traditional foods derived from β - galactosidase activity of lactic acid

Selecting a lactic acid bacterium cultured in MRS broth for β - galactosidase activity when disclosed number ONPG β - galactosidase activity (Sigma, USA) after confirming result when disclosed number using a solution, DK128, DK207, DK211 DK212 100 unit/mL or more excellent activity and the bix, number of β - galactosidase activity remaining strains [...] DK strains on other strains is lower than when disclosed number differentiation as signal peptides (2 also).

1 - 3. Selecting difference derived from Korean traditional foods 2 of lactic acid

1 Lactic acid based on number of physiologically active and β - it is cauchy writing base 36 species difference selecting excellent vitality ([...] seconds) be expected DK109 average fermentation of agricultural, DK119, DK121, DK128, DK207, DK211, DK212, DK215 8 2 difference selecting his total lactic acid bacteria species.

In the embodiment 2. Selecting optimal culture medium of lactic acid development and optimum condition setting

Biochemical characteristics and functional, animal milk [...] seconds for immunity-enhancing moieties DK128 lactic rates applications, DK207, a DK DK212 DK211 and optimized by applying growth force medium self development JMO strains has been confirmed.

50 Ml culture medium 24 to 37 °C JMO in time after inoculation by a 1 ml each strain (DK128, DK207, DK211 and DK212), 0 h, MRS agar medium dilution to the sampled time total [...] triazines crushing 24 were measured. As a result after culturing, DK128 is 3. 85 × 10⁹ CFU/mL, DK207 is 3. 90 × 10⁹ CFU/mL, DK211 is 2. 89 × 10⁹ CFU/mL, the DK212 2. 85 × 10⁹ CFU/mL showed decreased. 10 Both said 4 strain⁹ At least JMO CFU/mL both suitable growth medium has been confirmed that the active force.

In the embodiment 3. [...] seconds Before fermentation, after Antioxidant ability Change comparative analysis

Post-harvest prong [...] seconds (lifestyle beginning) after freeze-drying are grinded using a stand-alone level number 10 provided 20 mm long grinding work through powder using industrial mixer [...] seconds. For the milk to efined [...] seconds based on manufacturing method in the embodiment 6.

[...] seconds before fermentation, total flavonoid content change after a long time. [...] seconds before fermentation, a sample methanol, 10% AlCl₃ , 1M potassium acetate, at room temperature 30 minutes set by reacting with gun toe meter 415 nm absorbance spectral UV/VIS distilled water into and out of the total flavonoid content were measured. As a result, total flavonoid content as follows [...] seconds in addition result is higher than the regulated milk (milk [...] is corrosion-seconds) has been, in particular in the present invention using the best DK128 isolated probiotic activity by fermenting the fermentation sample controls are flavonoid content higher than before sample [...] seconds as well as fermentation and viscoelasticity (3 also).

In addition, [...] seconds before fermentation, total polyphenol content change after a long time. 0 Sample [...] seconds before and after fermentation. 2M reagents at room temperature 30 minutes after reacting a sample 10% Na N-a Folin₂ CO₃ Through the 700 nm absorbance spectral UV/VIS 1 total polyphenol content with gun toe meter set has been completed at room temperature were measured. As a result, regulated and [...] seconds before fermentation sample containing [...] seconds before fermentation compared to total polyphenol content before fermentation sample containing the difference invisible but, in particular DK128, DK207, DK212 DK211 and respective check number after fermentation containing total polyphenol content than fermented [...] seconds when the bath sample fabrics (4 also).

In addition, [...] seconds before fermentation, antioxidant capability change after a long time. Sample filled in 30 minutes UV/VIS [...] seconds before reacting with gun toe meter 37 °C fermentation DPPH reagent to be set to 510 nm absorbance spectral absorbance were measured. As a result, anti-oxidation activity regulated [...] seconds before fermentation sample prepared as before to go invisible but in particular DK128, DK207, DK211 DK212 [...] seconds using DPPH value is regulated and fermentation of fermentation and each sample before tends and viscoelasticity (also 5) increased.

Consequently, after fermentation of TPC [...] seconds, TFC and antioxidant potency viewed tendency is increased antioxidant activity is mainly composed of a material having excellent antioxidant flavonoids phenolic components so been determined.

In the embodiment 4. [...] seconds Before fermentation, comparative analysis after component change

[...] seconds before fermentation, component change after a long time. Fermentation controls and fermentation [...] seconds before, receives the comparison of HPLC chromatography sample was. Indicator component is isorhamnetin provided 3 a-O-a β-a galactosyl-a 4 '- O-a β-a glucoside (1) on isorhamnetin provided 3 a-O-a β-a glucosyl-a 4' - O-a β-a glucoside (2) 0.50 to, 13 each. 7 The heater 14. 5 Component peak appeared.

Before fermentation [...] seconds, after which the amount of glucose after fermentation glucose amount before reducing main the saccharide glucose fermentation has been confirmed (6 also). This strains during fermentation using glucose fermentation c [...] seconds DK128 proliferating and been analysis.

In the embodiment 5. [...] seconds Before fermentation, activity change after comparative analysis

[...] seconds before fermentation, in order to compare activity of 4 kinds of pathogenic microorganisms causing object comparison his database. KCTC registered pathogenic microorganisms are Escherichia coli (E. Coli KCTC 1682), Staphylococcal (S. Aureus KCTC 3881), faecalis (E. Faecalis KCTC 2011) and bacillus cereus (B. Cereus KCTC 3624) KCTC regardless from to as well as experiments. 1% To 35 °C TSB medium (v/v) level for the approved strains in culture nor post-seeding 24h TSA solid medium before fermentation, the supernatant of a sample takes paper well (10 mm) to be inoculated with a sample size was 200 micro l to a largest inhibition annularity comparing dissolved. As shown in the below table 2, DK128 [...] seconds corresponding motor is crucial to containing sample is dissolved in controls relative to 24.4, bacillus cereus fermentation than before after fermentation is to Staphylococci and for ETEC is dissolved.

Said result before fermentation, [...] seconds after activity of pathogenic bacteria compared to paper well (10 mm) from inhibiting annularity size (1 a-2 mm: +, 2 provided 4 mm: + +, 4 a-8mm + + +) precursor according, is NE activity that is not effective for a return electrode substrate.

In the embodiment 6. Immunity-enhancing animal [...] seconds Number article producing milk (lactic acid)

6 - 1. Establishing optimum condition of immunity-enhancing animal milk [...] seconds

6 - 1 - 1. Animal immunity-enhancing [...] seconds set raw material milk

(Main) to receive a supply line bio industry in a number prong [...] seconds (lifestyle beginning) 10 provided 20 mm size beyond the periphery of the joint cooperation only freeze-drying of PET after sternotomy [...] university industry and education in the cabin (OPERON, OPR-a FDV provided 7012) for supplying a 1AH, 12A, 24h -70 °C 10Hz to long term industrial mixer setting positions and keeping the sample as well as the sealing -24 °C freezer. Number jaws [...] seconds for removal of fat gushing oil Skim milk Preparation immunity-enhancing animal milk (SMP8515, Schils Food, B. V. Holland) for removal of fat gushing oil feed supply of 0.50 to experiments, proliferating the number of lactic acid was used article 'DEAHAN' yarn crystallized glucose function.

6 - 1 - 2. Animal milk [...] seconds immunity-enhancing combination ratio setting

A fermented milk grinding [...][...] seconds 0 seconds. Upon addition of 4% or more during fermentation [...] seconds due not made due to agglomeration problem generated in a curd muco polysaccharide in fermentation. The processing a grinding [...] 0. 1, 0. 2, 0. 3% Milk was added to a high pressure liquid coolant number verifying functional and effective. In addition 0. In maintaining a viable count of milk containing 2% hereinafter [...] seconds grinding capacity 0. Its effect is then combined at a 3% as compared to the last table 3 has been obtained by such as waste. The car number is 1 × 10 DK128 Lactobacillus strains⁶ To 1 × 10⁷ So cfu/ml was inoculated.

6 - 1 - 3. Animal milk [...] seconds total polyphenol content and antioxidant capability analysis for immunity

Animal milk [...] seconds total polyphenol content of the immunity-enhancing his (also 7). 0 Milk sample which has been pretreated [...] seconds. 2M reagents at room temperature 30 minutes after reacting a sample 10% Na N-a Folin₂ CO₃ Through the 700 nm absorbance spectral total polyphenol content after UV/VIS 1h with gun toe meter set at room temperature were measured. Animal immunity-enhancing [...] seconds [...] seconds total polyphenol milk tends significantly according increased unsaturated fatty acid content increased unsaturated fatty acid content and viscoelasticity.

6 - 1 - 4. Immunity-enhancing antioxidant (DPPH) analysis employed by animal milk content [...] seconds

0 Milk sample which has been pretreated [...] seconds. 02 Mm DPPH solution at room temperature by reacting with gun toe meter 517 nm UV/VIS 30min adding spectral absorbance (%) set by the erase DPPH employed for a long time. Animal immunity-enhancing [...] seconds [...] seconds to form milk increased unsaturated fatty acid content (%) prone to increased significantly DPPH erase function and viscoelasticity (also 8).

6 - 1 - 5. (FRAP) analysis employed by animal immunity-enhancing antioxidant milk content [...] seconds

Milk which has been pretreated with gun toe meter 593 nm absorbance spectral UV/VIS FRAP [...] seconds after sample reaction in solution (%) set by the FRAP 30min group erase function value measures the contrast of Troloc concentrate have shown. Animal milk [...] seconds content difference according to invisible but significant immunity-enhancing [...] seconds, [...] seconds but significantly high antioxidant ability (also 9) compared to controls is shown.

6 - 1 - 6. Milk content (ABTS) analysis employed by animal immunity-enhancing antioxidant [...] seconds

After reacting at room temperature milk sample which has been pretreated with gun toe meter absorbance spectral UV/VIS 6min [...] seconds adding ABTS stock solution set by the ABTS (%) value measures the contrast of Troloc concentrate 734 nm erase function shown. The ABTS [...] immunity-enhancing animal milk seconds increased unsaturated fatty acid content increases and viscoelasticity prone to erase at the side of a [...] seconds (10 also).

6 - 1 - 7. Animal immunity-enhancing [...] seconds according to viable count change during milk content storage

Animal prepared by the number 56 7 measuring viable count change milk immunity-enhancing [...] seconds during grinding result is 0 [...] seconds during storage. 10^1 Or more viable count than adhesively bonded ratio of 3% milk glumate forming capacitor has been confirmed. This number to number 2 year later physical and circulation number article width development will be reproduced locus bench and the number of the product circulation date and stability will significantly contribute to been analyzed (11 also).

In the embodiment 7. Animal immunity-enhancing [...] seconds when milk number article Pig Experimental product specification

For yearly cancer immunity [...] seconds animal milk effects have to manage ch'onan University (Professor one eastern fortune), 30 June 2016 from between 1 April 2016 to the experimental is conducting. Processing a reason of Pig with character money is added to the growing bacillus fermentation [...] assessing occurrence of diarrhea and swine respiratory disease immune measuring productivity electric effect for reducing malodor in effect be examined by measuring money in his embodiment Pig manure environment.

7 - 1. Test animals and designing experiments

Average weight 7. 22 ± 0. 04 Kg ([Yorkshire × Landrace] × Duroc) money to the growing around the X-axis 240 deduced species in terpolymer, after reason (4 main), growing electric (4 main) total 8 to main conducting during testing specifications. 4 Iteration by each processing in accordance with the weight per total 3 processing money room was arranged so as to surround two 20 [...] (RCBD; Randomized Complete Block Design). Processing a configuration 1) Con: matching opening 2) T1: Pig route pasture added 1% 3) T2:1% milk such as gates of the pasture [...] seconds added to the table in 4.

1) Con: matching opening 2) T1: Pig route pasture added 1% 3) T2: milk added 1% pasture [...] seconds

7 - 2. Specification management

The contrast and the matching opening feed feed product entering Con existing T1 and T2 is added 1% 1% milk feed [...] seconds a Pig feed route class hereinafter been class hereinafter. Switching plate is coupled feed, water is free-living on vegetables psfv feeding auto fallen, other specifications it will be a wind farm management standard specification pigpens been satisfying quality control method.

7 - 3. Investigation and method

Week 1 times daily allowance (ADG) measuring body weight and feed intake from disclosure experiments have the productivity amount, daily allowance feed intake (ADFI) and feed efficiency (G: F ratio) calculated by the productivity effect [...] seconds in Young Pig milk feed efficiency were measured. Feed intake is measured when the remaining total salary amount between the receiving every each, were measured using the productivity daily allowance for the amount of money elder brother (movable Pig weight dedicated balance). Feed request rate of the pin between the productivity amount tires are a feed intake. The whole experiment 2 times to 10 - 11 morning when monitoring a clinical episodes qualifications with respect to respiratory symptoms are observed to fire extinguisher conditions and respiratory conditions and server stores generated diarrhea frequency of occurrence were measured. The morning when measuring pollutants smell of face powder 10 - 11 1 10 by two main observing any clinical episodes [...] processing times caused by arthritis [...] moieties after taking a plastic container which is included in the Gastec 100g (Model GV-a 100, GASTEC, Japan) through a hydrogen sulfide concentration ammonia concentrations were measured.

7 - 4. On porcine blood chemical apparatus and IFN-a r TNF a-a, IL provided 6 measuring

The use of a separate milk swine influence on 24 - 25 formed around the scan intervals and the X-axis 20 by any one zero Young Pig 300 experiments EDTA tube for detection after taking a blood processing [...] two centrifuging him. The reason for detection in the plasma to convert any blood [...] around each processing [...] 20 have tested on blood serum centrifuging blood chemical measure IFN-a r TNF a-a, even though the main and 8 embodiment 3 times each content measurement is IL provided 6 4 experiments experiments were measured (IDDEX yarn ELISA kit) main to concentration in the blood.

7 - 5. Productivity

Specifications the productivity daily allowance amount based on the experiment period, daily allowance in processing section but significantly differential output of feed intake, the productivity daily allowance amount 523 from the group consisting of milk processing [...] seconds. 2G and to milk the productivity higher than the regulated amount in control nine mistletoe. In milk processing job [...] seconds feed efficiency point controls and Pig for processing life [...] seconds yogurt processing regions are T2 appears to be relatively low so as bottom up (P<0. 05). As a result since the productivity of swine blood circulation [...] milk and milk product to effect an electric cell and growing ultra feed product containing Pig growth performance is higher than the daily allowance but exhibit Pig is other processing so as to surround the feed intake [...] seconds compared to milk processing device a lower intake opening is shown as a carbon-high feed efficiency purges. Experiment compared to milk and end at the time of weighing result compared even been increased (table 5) opening [...] seconds processing device weight.

1) Con: matching opening 2) T1: Pig route pasture added 1% 3) T2: milk added 1% pasture [...] seconds

** Pooled standard error

In contrast to significantly lower the productivity daily allowance amount 4 5 6 but the productivity amount parking and parking population tends recovery from 4 - 5 fire extinguisher and respiratory symptoms was this main between main exhibit relative to expression of milk in a milk processing that a processing group [...] seconds according to the amount of porcine circovirus disease defense showing the [kkwu under it gives reason late and the productivity daily allowance group appears that only small part been analyzed electric growing effect. In the milk and milk [...] seconds added systems influence on productivity swine feed intake show significant difference to all processing section but the highest result at an contrast him money. The group consisting of milk in milk processing group [...] seconds feed efficiency is high in comparison with a series of many processing appears to be regulated and made, in particular, from the group consisting of milk processing step 6 is compared to previously grown regulated [...] seconds initial testing period is lower than the opening appears to be most but regulated parking contrast compared with consistently high milk processing kind appeared.

7 - 6. Generating defense effect [...] disease

Reason for table 6 and table 7 expression rate [...] diarrhea and respiratory conditions have shown the results of a comparison. Milk and milk [...] piglet feed addition reason the table 6 on a likely influence on diarrhea ultra [...][...] seconds added to milk as the lowest at an diarrhea likely 24.4, in milk processing device then showed low levels (P=0. 05). This specifications [...] seconds (T2) at each growth of highest capability that milk [...] furniture purges. However was not consistent result appears in milk opening of seafood added sexual specifications. In addition table 7 as in expression of respiratory symptoms or conditions involving fire extinguisher rate is lower than the effect appears to be off. Expression of respiratory conditions compared to a low relative dielectric constant compared both [...] seconds and milk processing device opening order appears to be blocking respiratory disease fails ETEC [...] seconds only a sliding milk product.

1) Con: matching opening 2) T1: Pig route pasture added 1% 3) T2: milk added 1% pasture [...] seconds

1) Con: matching opening 2) T1: Pig route pasture added 1% 3) T2: milk added 1% pasture [...] seconds

7 - 7. Yogurt is minute within arm [...] seconds influence of harmful gas generation

Test for money [...] seconds and 8 while adding milk feed main intervals caused by Pig ordure [...] 2 times 4 daytime product be used as the amount of harmful gas in table 8 as disclosed. First ammonia (NH₃ ) The amount of milk processing port T1 and T2 respectively processing device 11 milk processing job [...] seconds. 2 Ppm and 9. The contrast can be effectively shown relative to lower opening 6 ppm (p<0. 05) Even number of swine billion harmful gas purges put into minute within arm milk.

1) Con: matching opening 2) T1: Pig route pasture added 1% 3) T2: milk added 1% pasture [...] seconds

On the other hand, hydrogen sulfide (H₂ S) T1 and T2 processing each gas inlet 8. 1 Ppm and 5. The matching opening 8 ppm hydrogen sulfide is effectively reducing amount of milk processing job [...] seconds compared to T2 can be ETEC. Pig milk [...] seconds minute within arm harmful gas analysis results than oral administration of recombinant porcine removing ammonia and hydrogen sulfide reducing sewage hog farm billion harmful gas generation number been evaluated as effective. Oil number occur through dietary control article may be obesity management and prevention, prevention and treatment are used as constituents of enhancing health have exclusively of vehicle from the outside. The number food to food with constituents of milk and oil product utilizing a continuous positive studies will be in progress.

7 - 7. [...] seconds yogurt is an influence on immune response

Pig immune response when milk [...] seconds is absorbed through an influence on pasture after 2 separate experiments after blood sampling including INF-a γ TNF provided a main, every substance characterized in that inflammation such as inflammatory IL-a 6 billion effectively measures the number has been confirmed (table 9) to detect whether or not. In the blood including TNF a-a INF-a γ, IL-a 6 high antioxidant activity measured material from the group consisting of milk [...] seconds processing and generation of inflammatory mediators billion number the number of passes is excellent anti-inflammatory peptides. Number of locus in both basic research, enzyme number, yeast and antibiotic number at the time of the productivity by feeding the same amount and fodder is able from a piglet growth stimulating number demonstrated the possibility as improved efficiency locus number corrosion disclosed. Weight gain and feed efficiency for processing a composite containing a number [...] locus in yeast as well as raising NH₃ And H₂ S breeding environment by reducing the occurrence of gas effect appeared.

1) Con: matching opening 2) T1: Pig route pasture added 1% 3) T2: milk added 1% pasture [...] seconds

** Pooled standard error

7 - 8. Experiment result abstract animal specifications

The experiments of Pig milk and milk added feed addition [...][...] seconds and disease defense effect and an influence on productivity of night embodiment for measuring ammonia generating hydrogen sulfide was. Experiments matching opening (C), matching opening Pig feed route 0. Adding 1% to the ball (T1), milk natural seconds 0. Adding 1% (T2) to the ball are two processing hole such as 3, 4 per processing repeated, by reason of the two total 240 was repeated 20 per two [...] embodiment the X-axis. The feed intake amount [...] seconds compared to milk processing device opening the productivity result experiment compared significantly (P<0. 05) Pressure was high, compared to the amount of milk processing ports opening slightly improving the productivity (C) contrast the machining statistical significant difference have not been provided. All processing section feed request rate significantly difference him money. Observing [...] seconds at an added result milk discriminates whether a milk processing to cope with the disease most likely lower 24.4 diarrhea, in milk processing device then showed low levels (P=0. 05). The amount of milk and milk processing device both [...] seconds compared to ammonia gas is lower than the opening as shown in the same number of milk is the incidence ammonia gas purges.

The resulting composite enzyme on addition of reason [...] improve productivity locus number number number generation billion to lack of ammonia gas. As a result of the enzymatic secretion [...] seconds more milk is normal fire extinguisher tube which maintains the function of microorganisms using nutrient feed smoothly and the rotation effect the productivity of money amount and fodder with character money growing reason to increase the efficiency of ETEC. In addition hydrolyzing milk milk [...] seconds compared to general reason with character money exerts anti-oxidant and inflammatory effects of diarrhea and respiratory disease in [nyo containing more land and lowers the growing money with minute arm of Pig breeding industry and other industrial applications by reducing the amount of ammonia and hydrogen sulfide gas hog farm solve environmental pollution around door number who purchase lottery tickets show article to determine the other.