5-NITROFURAN DERIVATIVES

PROCESS AND DEVICE FOR THE DETECTION OF PATHOGENS

There is provided a process for the detection of pathogens, such as bacteria, fungi and viruses, in blood in the presence of an anticoagulant agent, wherein the pathogen is separated in an extracorporeal circulation of the blood with a biocompatible adsorbent which selectively binds the pathogen, whereafter the pathogen is detected by bacteriological, mycological, virological or electron microscopic methods. A particular device for the detection of pathogens in blood includes a column, both ends of which are open, which contains a biocompatible adsorbent which selectively binds the pathogen, the adsorbent being held in place in the column by means of filters, grids or meshes with slipped-over caps, the caps having openings with connections for tubes for admitting or removing blood. The tubes for removing blood can also be provided with a transfusion device and a filter.

PROCESS AND REAGENT FOR THE COMPLETE AND RAPID REMOVAL OF A TURBIDITY IN A BIOLOGICAL FLUID

The present invention provides a process for the removal of turbidities in biological fluids by the addition of surface-active agents, wherein, as surfaceactive agents, there are used a) a polyethoxylated triglyceride with an HLB value of 4 to 14, b) a secondary n-alkane sulphonate, as well as optionally c) a further non- or anionic tenside, in aqueous, optionally buffered solution. The present invention also provides an agent for the removal of a turbidity in a biological fluid, wherein it contains a) a polyethoxylated triglyceride with an HLB value of 4 to 14, b) a secondary n-alkane sulphonate, as well as optionally c) a further non- or anionic tenside, in aqueous, optionally buffered solution.

NUCLEOSIDE TRIPHOSPHATE-DEPENDENT 1-METHYL- HYDANTOINASE, A PROCESS FOR OBTAINING IT AND THE USE THEREOF

A nucleoside triphosphate-dependent 1-methyl-hydantoinase, a process for obtaining it and the use thereof The present invention provides 1-methylhydantoin-ase, which hydrolyses 1-methylhydantoin in the presence of a nucleoside triphosphate and of polyvalent metal ions. The present invention also provides a process for obtaining 1-methylhydantoinase and a reagent containing it. Furthermore, the present invention provides a process for the determination of creatinine by the conversion of the creatinine with creatinine deiminase (E.C. 3.5.4.21) into 1-methylhydantoin, hydrolysis of the latter with the 1-methylhydantoinase in the presence of nucleoside triphosphate and of polyvalent metal ions and determination a) of the hydrolysis product formed from l-methyl-hydantoin with N-carbamoylsarcosinamidohydrolase with formation of sarcosine and detection of the sarcosine with sarcosine oxidase or sarcosine dehydrogenase or b) of the simultaneously formed nucleoside diphosphate.

DEVICE FOR THE DETECTION OF THE PRESENCE OF AN ALLERGY AND FOR THE SPECIFIC DETECTION OF THE ALLERGEN RESPONSIBLE FOR THE ALLERGY

A device for the detection of the presence of an allergy or corresponding stimulation factor and fox the determination of anti-allergy substances includes a microtitre plate with rows of different reagents each of which comprises a buffer with a pH of from 6.3 to 9 with 0.5 to 5 x 10 3 mol/l. of calcium ions and 10 4 to 10 3 mol/l. of chromogen and a stimulation factor inducing protease liberation; the device can be used in the diagnosis of allergies and other diseases.

TEST STRIPS

A test strip comprises a film layer containing reagents and a support or handle member, particularly in the form of a synthetic resin film; the reagent film layer is preponderantly on one side of a multifilar carrier layer such as a fabric or fleece, between the film layer and the support there is preferably employed an additional absorbent layer, the components may be connected together by means of a thin, transparent mesh fixed to the support. A process for the production of such a test strip comprises applying a dispersion or solution of the components forming the reagent film layer in an appropriate solvent, to one side of the carrier layer and drying to form the film layer, whereafter this is connected in per se known manner with the other components of the test strip and, if desired, cut up into appropriate smaller units.

PIPERIDINOPROPYL DERIVATIVES

The present invention provides piperidinopropyl derivatives of the formula: wherein R1 and R2, which can be the same or different, are hydrogen atoms or lower alkyl radicals or together represents an alkylene radical, R3 is a hydrogen atom or-O-R5, R5 being a hydrogen atom or an acyl radical,R4 is a hydrogen atom or a formyl radical, A is an -X1=Y1-grouping, in which X1 and Y1, which can be the same or different, are nitrogen atoms or -C(R6)=, in which R6 is hydrogen atom, a lower alkyl radical optionally substituted by -O-R5, wherein R5 is as defined above a carboxyl or an alkoxy carbonyl radical; or A is an-X2-Y2- grouping, in which X2 is -CH2- or -N(R7)-, in which R7 is a hydrogen atom or a lower alkyl radical, and Y2 is -CH2- or -C(=Z)-, in which Z is an oxygen or sulphur atom; or A is a -CR8=CR9- grouping, in which R8 and R9 together represent a -CH=CH-CH=CH- bridge, with the proviso that Y1 or Y2 is connected to >N-R4 in the above-given formula, and B is a heterocyclic radical or, ...

PHENYLACETIC ACID DERIVATIVES AND THE PREPARATION THEREOF

Phenylacetic acid derivatives with which the present invention is concerned are compounds of the general formula (I) (I), wherein A is an aryl radical which is unsubstituted or substituted by one or more halogen, trifluoromethyl, alkyl, alkoxy, alkenyloxy, alkoxyalkoxy, alkyl-substituted amino or aryloxy, or a heterocyclic ring system which is unsubstituted or substituted by halogen, alkyl or alkoxy, Y is a valency bond or an unbranched or branched alkylene radical containing 1 to 3 carbon atoms and R1 and R2, which may be the same or different, are hydrogen atoms or lower alkyl radicals; and the physiologically compatible salts and esters thereof. The compounds and the physiologically compatible salts and esters thereof possess surprisingly outstanding hypoglycaemic and/or hypolipidaemic properties.

2-(PHENYLALKYLHYDRAZONO)-PROPIONIC ACID DERIVATIVES AND THE PREPARATION THEREOF

New propionic acid derivatives are provided which exhibit a hypoglycaemic activity at a dosage level at which an inhibition of monoamino oxidases does not occur or does not occur to a substantial degree; the new derivatives have the formula (I): (I) wherein R1 and R2, which can be the same or different, are hydrogen or halogen atoms, or trifluoromethyl, lower alkyl, hydroxyl, acyloxy or lower alkoxy radicals or together represent a methylenedioxy radical and X is a straight-chain, saturated or unsaturated aliphatic hydrocarbon radical containing 2 to 4 carbon atoms which is unsubstituted or substituted by a lower alkyl radical in the .beta.- or .gamma.-position to the nitrogen atom; and the pharmaceutically acceptable, physiologically compatible salts, esters and amides thereof; there are also provided processes for preparing the new compounds.

STABILISED UREASE

A stabilised urease is provided which contains, as stabilising agent, a mixture of glutathione, ethylenediamine-tetra-acetic acid and citrate, the stabilised urease may be impregnated in a carrier paper or carrier film and employed for the determination of ammonia.

O-SUBSTITUTED PYRUVIC ACID OXIMES, PROCESSES FOR THE PREPARATION THEREOF AND PHARMACEUTICAL COMPOSITIONS WHICH CONTAIN THESE NEW COMPOUNDS

The present invention provides pyruvic acid oximes of the general formula: (I) wherein R is a hydrogen atom, a C3 - C8 cycloalkyl, C1 - C6 alkoxy, cinnamyloxy, phenylamino, phenyl-N-alkylamino or phenylthio radical or an aryl or aryloxy radical, the aryl moiety of which can be substituted one or more times by C1 - C6 alkyl, C1 - C6 alkoxy, halogen, hydroxyl, trifluoromethyl, amino, acetylamino, nitrile, nitro or methylenedioxy, A is a straightchained or branched, saturated or unsaturated aliphatic hydrocarbon chain containing up to 10 carbon atoms, which can be substituted one or more times by halogen or hydroxyl,and R1 is a C1 - C8 alkyl radical, which can be substituted one or more times by halogen, hydroxyl, nitrile, phenyl or carboxyl, or is a nitrile or formyl group, with the proviso that R-A- is not a methyl, ethyl or benzyl radical; and the physiologically acceptable salts, carboxylic acid esters and amides thereof. The present invention also provides processes for the preparation ...

PROCESS FOR ANALYTICAL DETERMINATIONS AND ROTOR INSERT ELEMENT THEREFOR

The present invention provides a process for carrying out analytical determinations by mixing and incubating a sample solution with at least one reagent solution and optical measurement of one parameter in the incubated reaction solution mixture, mixing, incubating and measuring being carried out during the action of a centrifugal force, wherein, under the influence of the centrifugal force, the sample solution is brought together with a soluble dry reagent, with at least partial dissolving thereof, and the mixture or the solution is forced centrifugally through a plurality of small hollow spaces, the centrifugal force and the flow resistance of the small hollow spaces being so adapted with regard to one another that a complete dissolving and mixing of the reaction components and possibly incubation takes place before the reaction solution passes from the small hollow spaces into a measuring chamber in which the measurement is carried out. The present invention also provides an insert element ...

PROCESS FOR THE CARRYING OUT OF ENZYMATIC DETERMINATIONS

A process and reagent for carrying out enzymatic determinations in which NAD(P)H is formed and intermediate products and non-specifically-reacting disturbing substances are removed in a preliminary reaction, during which, there is simultaneously carried out an NAD(P)H-consuming enzymatic reaction which completely oxidises the NAD(P)H formed to give NAD(P); the NAD(P)H-consuming reaction is stopped at the start of the measurement reaction.

PROCESS FOR THE PREPARATION OF A TISSUE THROMBOPLASTIN

The present invention provides a process for the production or a tissue thromboplastin preparation which is sensitive towards coagulation Factor VII by the production of an acetone dry powder from mammalian tissues and extraction of the powder with salt solution, wherein the extraction is carried out with a salt solution which contains 1 to 20 mMole/litre of calcium ions and optionally a surface-active agent and the extract obtained is, if desired, dried. The present invention also provides a reagent for assessing blood coagulability with a content of thromboplastin and calcium salt, the thromboplastin having been obtained by the above process. If desired, the reagent can also contain a thromboplastin preparation which is insensitive towards Factor VII in an amount such that a predetermined Factor VII sensitivity is present.

PROCESS FOR THE PREPARATION OF 1,4:3,6-DIANHYDRO-D- GLUCITOL 5-NITRATE

The present invention provides a process for the preparation of 1,4:3,6-dianhydro-D-glucitol-5-nitrate, in which, in a particular embodiment. a) 1,4:3,6-dianhydro-D-glucitol is acetylated with 0.5 to 1.5 mole equivalents of acetic anhydride in the presence of a catalyst, a mixture of 1,4:3,6-dianhydro-D-glucitol-2-acetate, 1,4:3,6dianhydro-D-glucitol-5-acetate and 1,4:3,6dianhydro-D-glucitol-2,5-diacetate being obtained; b) the mixture is nitrated with nitric acid, a mixture of 1,4:3,6-dianhydro-D-glucitol-2-acetate-5-nitrate and 1,4:3,6-dianhydro-D-glucitol-5-acetate-2-nitrate being obtained, and c) hydrolysis is carried out with an inorganic base to give 1,4:3,6-dianhydro-D-glucitol-5-nitrate; wherein, in step a), the reaction is carried out in the presence of a base and the 1,4:3,6-dianhydro- D-glucitol-2,5-diacetate is substantially removed before further working up.

PROCESS FOR THE ENZYME IMMUNO DETERMINATION IN THE HETEROGENOUS PHASE

The present invention provides a process for the determination of a component of the reaction between a specific binding protein and a substance bindable therewith by incubation of a sample solution with a previously ascertained amount of binding component in insoluble form in the presence of a reagent which contains a known amount of one of the binding components in enzyme-labeled form, separation of the liquid phase from the solid phase by centrifuging and determination of the activity of the marker enzyme remaining in the liquid phase, wherein measurement of the enzyme activity is carried out in a centrifuge rotor during or after the action of increased gravitational force whereby the solid phase and the liquid phase are still in contact with one another.

PROCESS FOR THE ENZYMATIC DETERMINATION OF FREE AND ESTERIFIED CHLORESTEROL

Cholesterol is determined by incubating a sample suspectod of containing cholesterol in an aqueous medium with cholesterol oxidase and determining either the oxygen consumption, the hydrogen peroxide formed, or cholestenone, as a measure of the initial cholesterol content; reagent compositions comprising choloaterol oxidase and a system for the determination of H2O2 or for the determination of cholestenone are provided.

SULPHUR-CONTAINING PHOSPHOLIPIDS, THEIR PREPARATION AND USE

The present invention provides alkanol phosphoric acid monoammonium alkyl esters of the general formula: (I) wherein X is a valency bond, an oxygen or sulphur atom, a sulphinyl or sulphonyl group, an aminocarbonyl, carbonylamino or ureido group or a cycloalkylene or phenylene radical, Y is an oxygen or sulphur atom, R1 is a hydrogen atom, a straight-chained or branched, saturated or unsaturated aliphatic hydrocarbon radical containing up to 18 carbon atoms, which can be substituted one or more times by aryl, halogen, lower alkoxy, alkylthio, alkoxycarbonyl, alkanesulphiny or alkanesulphony, R2 is a straight-chained or branched, saturated or unsaturated aliphatic hydrocarbon chain containing up to 18 carbon atoms, which can be substituted one or more times by halogen, aryl, lower alkoxy, alkoxycarbonyl, alkylthio, alkanesuphinyl or alkanesulphonyl, R3 is a straight-chained or branched, saturated or unsaturated aliphatic hydrocarbon chain containing 2 - 0 carbon atoms, which can also ...

DERIVATIVES OF INDAZOLE AND THE PREPARATION THEREOF

New derivatives of indazole and their preparation are provided of the formula (I): (I) wherein R1 is a hydrogen atom or a lower alkyl radical. R' is a hydrogen atom or an acyl radical and R2 is a benzyl radical or a radical of the formula: in which X is a reactive residue and Y is a hydroxyl group or X and Y together represent an oxygen atom and, when R ' is an acyl radical, R2 can also be a hydrogen atom, the derivatives have been found to be valuable intermediates for the preparation of useful, pharmacologically-active compounds, for example, 3-indazolyl-(4)-oxy-propan-2-ol amines which can bring about an inhibition of adrenergic .beta.-receptors and can, therefore, be used for the treatment and propylaxis of cardiac and circulatory diseases.

AMINE DERIVATIVES OF 4-HYDROXY-2,1,3-BENZTHIADIAZOLE

New amine derivatives of 4-hydroxy-2,1,3-benzthiadia-zole of formula (I): (I) wherein R is a straight-chained or branched lower-alkyl radical, are provided, their pharmaceutically acceptable, pharmacologically compatible acid addition salts and processes for their preparation. The new derivatives of formula (I) bring out an inhibition of adrenogenic .beta.-receptors and are useful for the treatment and prophylaxis of cardiac and circulatory diseases.

DIGOXIN MONOMETHYL ETHERS

PROCESS AND REAGENT FOR THE SPECIFIC DETERMINATION OF PANCREATIC ALPHA-AMYLASE

Process and reagent for the specific determination of pancreatic alpha-amylase The present invention provides a process for the specific determination of pancreatic alpha-amylase in the presence of salivary alpha-amylase in body fluids, especially in serum, plasma, duodenal juice or urine, by reaction with a system for the detection of alphaamylase in the presence of a monoclonal antibody which inhibits salivary alpha-amylase / wherein, as inhibitor, there is used a first monoclonal antibody which specifically inhibits the salivary enzyme by less than 97%, in combination with a second monoclonal antisaliva alpha-amylase antibody which inhibits this enzyme by less than 10%. The present invention also provides a reagent for the specific determination of pancreatic alpha-amylase in the presence of salivary alpha-amylase in body fluids, especially in serum, duodenal juice, plasma or urine, containing a system for the detection of alpha-amylase and a monoclonal antibody against salivary alpha-amylase ...

TRICYCLIC AMINOMETHYL DERIVATIVES

NERIIFOLIN DERIVATIVES

PROCESS FOR THE DETERMINATION OF A POLYVALENT ANTIGEN AND REAGENT THEREFOR

The present invention provides a process for determining a polyvalent antigen by incubation with three different receptors, the first and third of which are present in liquid phase and with which the antigen is bindable, the second receptor is present in solid phase and is only bindable with a part of the first receptor and the third receptor carries a marking and does not cross-react with the first and second receptor, separation of the solid phase. From the fluid phase and measurement of the marking in one of the phases, wherein a solution containing the antigen is brought together with a first solid carrier material, which contains, impregnated or lyophilised, the first and the third receptor in removable form and, after removal of the receptors, the solution obtained is immediately contacted with a second solid carrier material which contains a second receptor in non-removable form, whereafter the liquid is separated from the second solid carrier material and the marking is measured ...

CARDENOLIDE BIS-DIGITOXOSIDES

The present invention provides cardenolide-bis-digitoxosides of the general formula: (I) wherein R1 is a hydrogen atom, a hydroxyl group or an acyloxy radical containing up to 3 carbon atoms and R2, R3 and R4, which can be the same or different, are hydrogen atoms, acyl radicals containing up to 3 carbon atoms or alkyl radicals containing up to 3 carbon atoms, with the proviso that the compounds contain at least one alkyl radical. The present invention also provides processes for the preparation of these compounds and pharmaceutical compositions containing them. Furthermore, the present invention is concerned with the use of these compounds for the treatment of cardiac insufficiency.

CHOLESTEROL STANDARD

This application relates to a non-woven fabric wherein the fibers of the fabric are bonded to each other and/or to another material in a plurality of equally spaced-apart spots, wherein the spots are the same size and are combined in a plurality of identical groups of not less than three nor more than five identical spots, each of each of said spots within each of said line beyond the end-spots of said group positioned along said line intersects and adjacent line along which an adjacent group of spots is positioned, and wherein the ratio of the distance separating the spots within each group of spots to the distance from an end-spot in each group to the next adjacent line along which an adjacent group of spots is positioned, is about 0.3 to about 3.0. This arrangement permits optimum adhesion with other material or consolidation of the fabric itself and also provides directionally neutral flexibility.

HETEROARYLOXYPROPANOLAMINES

The present invention provides heteroaryloxypropanolamines of the general formula: < IMG > (I) wherein A is a bi- or tricyclic heteroaromatic radical, which is optionally partly hydrogenated, X is a straightchained or branched alkylene chain containing 2 to 6 carbon atoms, R1 and R2, which can be the same or different, are hydrogen atoms or lower alkyl or benzyl radicals, R3, R4 and R5, which can be the same or different, are hydrogen atoms or lower alkyl, benzyl, formyl, lower alkanoyl, cyano, hydroxymethyl, lower alkoxycarbonyl or carbamoyl radicals or the divalent substituents sulphur or oxygen and R6, R7 and R8, which can be the same or different, are hydrogen or halogen atoms, lower alkyl, nitro, amino, lower alkylthio or lower alkoxy radicals or R6 and R7 together represent an optionally unsaturated trimethylene chain; and the pharmacologically acceptable salts thereof. The present invention also provides processes for the preparation of these compounds, as well as pharmaceutical ...

PROCESS AND A REAGENT FOR THE DETERMINATION OF BLOOD GLUCOSE IN HAEMOLYSED WHOLE BLOOD

Process and reagent for the determination of glucose in haemolysed blood The present invention provides a process for the determination of D-glucose in body fluids by reaction with ATP in the presence of hexokinase, glucose-6-phosphate dehydrogenase, NADP, Mg++ ions and buffer and measurement of the NADPH formed, wherein whole blood is mixed with 0.01 to 0.5% weight/volume of an alkyl sulphate or sulphonate or of an alkyl-aryl sulphonate, whereby the alkyl radicals can contain 8 to 32 carbon atoms and can contain one or more hydroxyalkylamino radicals as substituents and/or can be interrupted by one or more ether oxygen atoms and the alkyl-aryl radicals can contain 8 to 18 carbon atoms in the alkyl moiety, and optionally with a preserving agent, the said further reagents then being added directly. The present invention also provides a reagent for carrying out this method, wherein it contains alkyl sulphate or sulphonate or alkyl-aryl sulphonate, whereby the alkyl radicals can contain 8 ...

PROCESS FOR THE PREPARATION OF PERMANENT ANIMAL AND HUMAN CELL LINES AND THEIR USE

Process for obtaining permanently culturable animal and human cell lines and the use thereof. The present invention provides a process for obtaining permanently culturable animal and human cell lines by fusion of normal animal and human cells with biological components which bring about a culturability in vitro, wherein normal animal or human cells are fused with cell fragments of transformed cells which alone are not capable of multiplication and then cultured in a culture medium without selection substances. The cell lines thus obtained can be used for obtaining cell products and for testing active substances.

AGENT FOR THE DETECTION OF ESTEROLYTIC AND/OR PROTEOLYTIC ENZYMES AND WITH SUBSTRATES SUITABLE FOR THIS PURPOSE

The present invention provides an agent for the detection of esterolytic and/or proteolytic enzymes, consisting of an absorbent carrier, a film layer, a powder mixture, a lyophilisate, a solution or a reagent tablet containing an appropriate esterase and/or protease detection principle, a buffer and optionally further conventionally used additives, wherein the esterase and/or protease detection principle used is a combination of a specially substituted diazonium salt and an appropriate ester, the reactivity of which is mutually coordinated by appropriate selection of the substituents in such a manner that a sufficiently rapid coupling takes place with the alcohol component liberated from the ester but no reaction with other components of the test solution occurs. The present invention also provides new esters, which are substrates for esterolytic and/or proteolytic enzymes, said esters being compounds of the general formula:- < IMG > in which R"1, R"2, R"3 and R"4, which may be the same ...

4-PHENOXYMETHYLPIPERIDINE COMPOUNDS

New intermediates and their preparation are provided of formula (I): (I) wherein R is a hydrogen or halogen atom or a nitro group or a lower alkyl or alkoxy radical; and the acid addition salts thereof with inorganic and organic acids; the new compounds are useful in the preparation of compounds with pharmaceutical properties, for example, with anti-allergic, and central nervous system-depressing action.

PURINE DERIVATIVES

New piperidinoalkyl derivatives of purine of the formula (I): wherein R1 is a hydrogen atom or a lower alkyl radical, R2 is a hydrogen atom, an aryl or a lower alkyl radical which is optionally substituted by hydroxyl or R1 and R2, together with the nitrogen atom to which they are attached, can also form a heterocyclic ring and R3 is a hydrogen atom or an acyl radical; and the acid addition pharmaceutically acceptable, pharmacologically compatible salts thereof with non-toxic inorganic or organic pharmacologically acceptable acids have anti-allergic, anti-inflammatory and anti-oedematous activities.

PROCESS FOR A REAGENT FOR THE DETERMINATION OF BIOLOGICALLY-ACTIVE HEPARIN IN PLASMA

A method and apparatus for lining a pipe, e.g. an aluminium pipe, with a thermoplastic material, in which either the inner surface of the pipe or the external surface of a tube of thermoplastic material is coated with an adhesive layer, the tube is inserted in the pipe, a purely radial force is applied to press the plastic tube against the inner surface of the pipe, and axially progressive heating is applied to render the thermoplastic material into a plastic state.

PROCESS FOR THE PREPARATION OF (ALPHA)-(4-(4- CHLOROBENZOYLAMINOETHYL)-PHENOXY)ISOBUTYRIC ACID

A new process for preparing 2-¢4-(4-chlorobenzoylamino-ethyl)-phenoxy!-2-methylpropionic acid of the formula: and salts thereof, comprising reacting N-(4-chlorobenzoyl)-tyramine,acetone, chloroform and alkali at a temperature of from about 10 to 40.degree.C., in a molar ratio of N-(4-chloro-benzoyl)-tyramine to acetone to chloroform of 1:20 to 100:2 to 10, and 3 to 4 moles of alkali per mole of chloroform, the compound has lipid- and cholesterol-lowering action the new process is more economic and produces a greater yield.

PROCESS AND REAGENT FOR DETERMINATION OF URIC ACID

Uric acid in biological material is determined by means of uricase, catalase and aldehyde dehydrogenase in the presence of alcohol and nicotinamide-adenine-dinucleotide or phosphate (NAD(P), and at least one compound selected from oxalates, malonic acid mono(lower) alkyl esters, trihaloethanols, pyrazole and pyridine optionally substituted with one or more lower alkyl radicals and/or halogen atoms, pyridine-carboxylic acids optionally substituted by a lower alkyl radical and the amides and lower alkyl esters thereof, thiourea, isobutyramide and chelate-forming complexing agents; in this way there is provided a disturbance-free determination of uric acid in, for example, serum and plasma, which may be important in, for example, the clinical investigation of gout.

1-(3-(NAPHTH-1-YLOXY)-2-HYDROXY-PROPYL)-PIPERAZINE DERIVATIVES

New 1-¢3-(naphth-1-yloxy)-2-hydroxypropyl!-piperazine derivatives and their process of preparation are provided of the formula: (I) and their pharmaceutically acceptable, pharmacologically compatible acid addition salts; the new derivatives possess anti-hypertensive properties.

PARTICULATE IMMOBILIZED ENZYME ADHESIVELY BOUND TO FORMED BODY

Enzyme containing bodies are provided useful in research, industry and medicine; the bodies comprise formed bodies with an enzymatically-active surface, wherein the surface of a formed body is at least partially coated with adhesive and on the adhesive layer there are present particles which contain at least includes synthetic resin, metal, glass or a synthetic or natural polymer.

3,3',5,5'-TETRAALKYLBENZIDINES AND THEIR USE AS INDICATORS IN DIAGNOSTIC AGENTS

A diagnostic agent for the determination of hydrogen peroxide or of substances which react with the formation of hydrogenperoxide or of peroxidase or of peroxidate-active substances, employs the reaction of hydrogen peroxide with a chromogen, which reaction is catalyzed by peroxidase or a peroxidate-active substancas, and evaluation of the coloration producedby the reaction, the diagnostic agent employs, as chromogen, a compound of formula (I): in which R1, R2, R3, and R4, which can be the same or different, are straight-chained or branched alkly radicals containing 1 to 6 carbon atoms; the chromogen employed gives more stable coloured radicals in the test than o-tolidine.

CYCLOPENTENO-QUINOLONE DERIVATIVES

New cyclopenteno-quinolone derivatives, and their preparation, of the formula(I): (I) wherein R is a saturated or unsaturated aliphatic lower-hydro-carbon radical, X is a hydrogen atom or a lower alkyl radical and Y is a halogen atom or an azido, lower-alkylthio, loweralkyl-sulphonyl or nitrile radical or an unsubstituted or substituted amino group, and the pharmaceutically acceptable and pharmacologically compatible salts thereof, the derivatives in comparison with the known compounds have about the same antimicrobial action in vitro but a substantially stronger action in vivo, especially in the urinary tract.

1-(3-(NAPHTHYL-1-YL-OXY)-PROPYL)-PIPERAZINE DERIVATIVES

New 1-¢3-(naphth-1-yloxy)-propyl!-piperazine derivatives and their preparation of the formula (I): (I) wherein X is a hydrogen atom or a methoxy radical; and the pharmaceutically acceptable, pharmacologically compatible acid addition salts thereof; the derivatives possess outstanding blood pressure-lowering and thus anti-hypertensive properties.

BIS-(2,4-DINITROPHENYL)-METHYL-PYRIDINIUM COMPOUNDS AND THE PREPARATION THEREOF

New bis-(2,4-dinitrophenyl)-methylpyridinium compound useful as indicators in the detection of ammonia, for example, ammonia liberatea from urea in body flulas, are provided of the formula (I): (I) wherein one of R1 and R2 represents a bis-(2,4-dinitrophenyl)-methyl radical and the other represents a lower alkyl radical, or, in the case of R2, can also represent a hydrogen atom or a trifluoronethyl radical, R3 ana R4 are hydrogen atoms or, together with the carbon atoms to whlch they are attached, represent a benzene ring and X ? is an acid anion and the betaine form thereof.

PROCESS AND A DEVICE FOR THE PRODUCTION OF REAGENT SOLUTIONS

The present invention provides a process for the production of reagent solutions containing a plurality of components for analytical purposes, wherein the individual components of several differently composed reagents are each introduced into a solid, proportionable carrier material in easily redissolvable form in such an amount that each part by volume or weight of the carrier material carries a definite amount of one reagent component, the carriers of the components are brought together in a magazine-like manner and, for the production of a particular reagent, from all carriers which carry a component of the reagent, there are simultaneously taken off the amounts corresponding to the required amounts of the components, which are introduced into a reagent solution, in order to dissolve the components. The present invention also provides a device for the production of reagent solutions for analytical purposes which contain a plurality of components, comprising a magazine, which contains ...

TEST STRIP EVALUATING INSTRUMENT FOR MULTIPLE TEST STRIPS

A test strip evaluating instrument for multiple test strips used in the analysis of body fluids as an aid to diagnosis, has a transport and positioning device which transports the test strips at right angles to their longitudinal direction from a test strip feed area along a transport path to a disposal area; at least one measuring station with an optical measuring unit is arranged on the transport path; rails on which the test strips slide, run along the transport path; the test strips are transported step-wise by at least two rows of cam elements following one another in the transport direction, which move synchronously with one another in a periodically recurring movement path; the handling can be facilitated considerably without impairment of the measuring accuracy by the fact that the rails are designed as longitudinal profiling in a disposable transport insert made of a thin plastics material, the cam elements engage from above between the rails of the transport insert, a bearing ...

(5-INDOLYLOXY)-ACETIC ACID COMPOUNDS AND THERAPEUTIC COMPOSITIONS

PROCESS FOR THE ISOLATION AND PURIFICATION OF S-ADENOSYL METHIONINE AND ETHIONINE AND NOVEL SULFATES THEREOF

5-NITROFURAN DERIVATIVES, PROCESS THEREFOR AND COMPOSITION THEREOF

HETEROCYCLIC OXYPROPANOLAMINE DERIVATIVES

The present invention provides heterocyclic oxypropanol derivatives of the general formula: wherein R1 is a hydrogen atom or a lower alkyl, aralkyl or lower alkanoyl radical, R2 and R3, which can be the same or different, are hydrogen atoms or lower alkyl, hydroxyalkyl, alkoxycarbonyl or alkanoyloxyalkyl radicals or together represent an alkylene radical, R4 is a hydrogen atom or a lower alkanoyl radical or an aroyl radical, R5 is a hydrogen atom or a lower alkyl radical or an aralkyl radical, R6 is a hydrogen atom or a lower alkyl radical, R7 is a hydrogen atom, a hydroxyl group or a lower alkyl radical, Z is a valency bond, a methylene radical or an oxygen or sulphur atom, Ar is a carbocyclic aryl radical or a pyridyl radical, R8, R9 and R10, which can be the same or different, are hydrogen or halogen atoms, hydroxyl groups, lower alkanoyl radicals, lower alkyl radicals, lower alkenyl radicals, lower alkoxy radicals, aralkoxy radicals, allyloxy radicals, lower alkylthio radicals ...

PHENOXYALKYLCARBOXYLIC ACID DERIVATIVES

New phenoxyalkylcarboxylic acid derivatives and their preparation are provided of the formula (I) (I) wherein R is a hydrogen atom or a lower alkyl radical, R1 is an alkyl radical or an aryl, aralkyl or aralkenyl radical, the aryl moiety of which is unsubstituted or substituted one or more times by halogen atoms, hydroxyl groups, trifluoromethyl radicals or lower alkyl, lower alkoxy or acyl radicals, R2 and R3, which may be the same or different, are hydrogen atoms or lower alkyl radicals and n is 0, 1, 2 or 3, and the pharmaceutically acceptable, physiologically compatible salts, esters and amides thereof, the derivatives display a significant lipid-sinking action and exhibit an outstanding inhibiting action on thrombocyte aggresion.

DIGOXIN DERIVATIVES

The present invention provides 12-dehydrodigoxin oximes of the general formula: wherein R1 and R2, which can be the same or different, are hydrogen atoms, lower acyl radicals or lower alkyl radicals or R1 and R2 together can represent an alkylidene radical containing 2 to 6 carbon atoms and R3 is a hydrogen atom or a lower alkyl or aralkyl radical. The present invention also provides a process for the preparation of these oximes and pharmaceutical compositions containing them and is also concerned with the use of these oximes for the preparation of pharmaceuticals for the treatment of cardiac insufficiency.

CARBAZOLYL-(4)-OXYPROPANOLAMINE DERIVATIVES

New carbazolyl-(4)-oxypropanolamine derivatives and their process of preparation are provided of formula (I): (I) wherein R1 is a hydrogen atom, a lower alkanoyl radical or an aroyl radical: R2 is a hydrogen atom, a lower alkyl radical or an ar-lower-alkyl radical: R3 is a hydrogen atom or a lower alkyl radical, R4 is a hydrogen atom or a lower alkyl radical or, when X is an oxygen atom, together with R5 can also represent a -CH2-O- radical X is a valency bond. a-CH2- radical or an oxygen or sulphur atom: Ar is a monoor bicyclic hydrocarbon radical or a pyridyl radical and R5 and R6, which can be the same or different, are selected from the group consisting of hydrogen, halogen, hydroxyl, lower alkyl, aminocarbonyl, lower alkoxy, ar-lower-alkyloxy, lower alkylthio, lower alkylsulphinyl and lower alkylsulphonyl: or R5 and R6 together represent a methylenedioxy radical: and the pharmaceutically acceptable, pharmacologically or physiologically compatible acid addition salts thereof with non-toxic ...

PROCESS AND A REAGENT FOR THE ACTIVATION OF CHOLESTEROL ESTERASE

The present invention provides a process or activating cholesterol esterase in ion-containing solution by the addition of at least on surface-active agent, wherein there is useda) as surface-active agent, a polyethoxy ether or ester and/or a bile acid compound and/or a salt of a fatty acid containing 6 to 10 carbon atoms, in combination withb) a synergistically-acting alcohol selected from straight-chained, branched d and cyclic aliphatic alcohols which contain 5 to 12 carbon atoms, optionally halogen-substituted d aromatic alcohols and aliphatic alcohols which contain up to 3 carbon atoms and are substituted by several halogen atoms The present invention also provides an agent for the activation of cholesterol esterase in containing solution, comprising at least one esterase in ion-containing based upon a polyethoxy ether of ester and/or a bile acid compound and/or a salt of fatty acid containing 6 to 10 carbon atoms, together with a synergistically-acting alcohol selected from straight-chained ...

DERIVATIVES OF XANTHINE

The present invention provides xanthine derivatives of the general formula: wherein one of the symbols R1 and R2 stands for a methyl radical and the other for a radical of the general formula:- wherein R3 is a hydrogen atom or an acyl radical; and the pharmacologically acceptable salts thereof. The present invention also provides processes for the preparation of these compounds and pharmaceutical compositions containing them and is also concerned with the use thereof for the preparation of pharmaceutical compositions for the treatment of allergies.

ADENOSINE-5'-CARBOXYLIC ACID DERIVATIVES AND PROCESS FOR THEIR PREPARATION

Adenosine-5'-carboxylic acid derivatives of the formula: wherein R1 and R2, which may be the same or different, are hydrogen atoms, hydroxyl or amino groups or lower alkyl, lower alkenyl, optionally N-alkylated lower aminoalkyl, cycloalkyl or hydroxyalkyl radicals containing from 1 to 8 carbon atoms, preferably from 1 to 6 carbon atoms, or piperidine residues or R1 and R2 together represent a divalent alkylene radical containing 4 - 7 carbon atoms, which can be interrupted by an oxygen or sulphur atom or by an imino, alkylimino or arylimino radical and Z represents two hydrogen atoms or a lower alkylidene radical are prepared by reacting an appropriate adenosine-5'-carboxylic acid reactive derivative with an appropriate amine. The products and their pharmacologically compatible salts, preferably in the form of their pharmaceutical compositions are useful pharmacologically and exhibit a very strong circulatory action.

IMMUNESTIMULATING N-SUBSTITUTED AZIRIDINE- 2-CARBOXYLIC ACID DERIVATIVES

The present invention provides pharmaceutical compositions containing at least one aziridine-2-carboxylic acid derivative which is substituted on the ring nitrogen atom and which have the general formula: (I). wherein X is a carboxyl or nitrile group or an alkoxycarbonyl radical or an unsubstituted or substituted carbamoyl group; R is a straight-chained or branched, saturated or mono-or polyunsaturated aliphatic hydrocarbon radical which is optionally substituted one or more times by halogen, alkoxy, hydroxyl, dialkylamino, cycloalkylamino, acylamino, acyl, nitro, alkylthio, alkylsulphinyl, alkylsulphonyl, nitrile, carbalkoxy or carbamoyl radicals or by cycloalkyl radicals optionally substituted by alkyl, alkoxy or carbalkoxy, or by cycloalkenyl radicals, which can optionally be bridged, or by an aliphatic or aromatic heterocyclic radical, by aryl, aryloxy, arylthio, acyloxy, alkoxycarbonylamino or ureido groups, or R is a cycloalkyl or cycloalkenyl radical containing 3 to 10 carbon ...

PROCESS FOR THE QUANTIFICATION OF CELL POPULATIONS OR SUBPOPULATIONS AND A REAGENT SUITABLE THEREFOR

The present invention provides a process standard and kit for the quantification of cell populations or subpopulations, wherein a sample with the cell population to be determined is incubated with labelled antibodies which are specifically directed against characteristic surface antigens of the cell population to be quantified, one or more standards with known, differing particle concentration are incubated with the same labelled antibodies in the same way, the standard solution contains a mixture of particles which, in their sedimentation behaviour, are comparable with the cells to be determined, a first portion of said mixture comprising particles carrying a non-specific antibody and a second portion comprising particles carrying molecules directed specifically against the labelled antibodies or a part thereof, the cells of the sample solution, as well as the particles of the standard solution, are separated off from the excess labelled antibodies, the amount of the labelling is measured ...

REDOX INDICATORS

New redox indicators The present invention provides imidazole derivatives of the general formula: (I) wherein R is a hydrogen atom, a tetrahydrofuranyl, cycloalkyl or alkyl radical, which can be substituted by hydroxyl, alkoxy, a sulphuric acid, phosphonic acid or carboxylic acid residue, as well as by a phenyl radical, and R1 and R2, which can be the same or different, are julolidine radicals, tetrahydroquinoline radicals, which optionally carry on the nitrogen atom an alkyl radical which, in turn, can be substituted by a sulphuric acid, phosphonic acid or carboxylic acid residue, or radicals of the general formula: in which R4 is a hydroxyl or amino group or a monoor dialkylated amino group, in which the alkyl radicals can carry a sulphuric acid, phosphonic acid or carboxylic acid residue, and R3 and R5, which can be the same or different, are hydrogen atoms or alkyl or alkoxy radicals which are optionally substituted by a carboxyl group, with the proviso that R1 and R2 cannot ...

DIAGNOSTIC AGENT FOR THE DETECTION OF OCCULT BLOOD IN FAECES

Guaiaconic acid A is provided having a specific extinction E?Cm at 600 nm of at least 200, determined by the reaction with peroxidase and hydrogen peroxide, having infra-red bands at 1600 cm-1 (m): 1505 cm 1 (v.s): 1260 cm-1(s:b), 1115 cm-1 (m): 1025 cm-1 (m) and 1200 cm-1 (s:b) and having an RF value of 0.45 (toluene/dioxan/glacial acetic acid: 90:25:10 v/v/v); the acid is useful as a chromogen in diugnostic agents for the detection of occult blood in faeces.

DEVICE FOR THE OPTICAL IDENTIFICATION OF A CODING ON A DIAGNOSTIC TEST STRIP

The present invention provides a device for the optical identification of a bar coding on a diagnostic test strip in an evaluation device in which the test strip is positively movable relative to a reading device for the coding, with a source of light as a transmitter and a light-sensitive element as a receiver, wherein the bar coding has a high information density, between the test strip and the transmitter and/or between the test strip and the receiver there is, in each case, present a slit aperture and a cylindrical lens, the lens axis runs parallel to the test strip surface carrying the coding and to the code bars, the aperture slit lies in the plane defined by the cylinder axis and the transmitter or receiver and the distance between the lens axis and the test strip surface is substantially such that the focus lines of the lens lie approximately in the test strip surface.

PROCESS AND REAGENT FOR THE DETERMINATION OF TRIGLYCERIDES

Triglycerides are determined by enzymatic saponification with lipase in a buffer in the presence of a carboxylesterase and an alkali metal or alkaline earth metal alkyl sulphate, the alkyl radical of which contains 10 to 15 carbon atoms, wherein a compound of the general formula RO(CH2CH2O)nH, in which R is an alkyl or alkenyl radical containing 14 to 20 carbon atoms and n is a number of from 7 to 20, is also added.

PROCESS FOR THE PREPARATION OF SHAPED, COMPRESSED CONTROLLED-RELEASE UNIT-DOSAGE FORMS, AND THE COMPRESSED UNIT-DOSAGE FORMS THUS OBTAINED

A process is disclosed for producing moulded, pressed dose units with retarded r elease of a therapeutic active ingredient having compression pressure dependent self-retarding properties. In order to pro duce dose units having a very uniform. essentially linear and reproducible constant release of the active ingredient, the active in gredient is reduced during a first step to particles. with a fraction of an additive that inhibits or compensates its self-retarding p roperties. so that mouldings formed with these particles with no further additives have a rapid release in relation to the desired retard ed release in the pressure range required to compress the dose units. Then, during a second step. the particles are moulded w ith a retardant into dose units. Corresponding dose units are also disclosed.

INOSINE 2',3' ACETALS

N-SUBSTITUTED 1-AMINO-3-PHENOXY-PROPAN-2-OLS

New N-substituted 1-amino-3-phenoxy-propan-2-ols and their preparation are provided of the formula: in which R1 and R2 are hydrogen atoms or together represent a sulphur atom, an ethylene radical or a valency bond; B is a straight or branched chain alkylene chain containing 2 to 5 carbon atoms, and is unsubstituted or substituted by a hydroxyl group; and R3 is a hydrogen atom or a lower alkyl radical containing 1 to 3 carbon atoms; and the pharmacologically compatible salts thereof; the new compounds have valuable cardiac and circulatory properties.

PROCESS AND REAGENT FOR THE DETERMINATION OF THE ACTIVITY OF CREATINE KINASE-MB

The present invention provides a process for the determination of creatine kinase-MB in serum by the immunological elimination of subunit M and measurement of subunit B by methods known for the determination of creatine kinase, wherein subunit M is eliminated by adding to the test sample monovalent fragments obtained from antibodies against subunit M by proteolytic splitting under reducing conditions, whereafter subunit B is measured by methods known for the determination of creatine kinase. The present invention also provides a reagent for the determination of creatine kinase-MB, comprising a system for the determination of creatine kinase-MB and an agent for the immunological elimination of subunit M of the creatine kinase, the agent for the immunological elimination consisting of monovalent fragments of antibodies against creatine kinase-MM.

DIAGNOSTIC AGENT FOR CHOLESTEROL AND CHOLESTEROL ESTERS

A diagnostic agent for the detection and determination of cholesterol and cholesterol esters in body fluids comprises an absorbent carrier or a synthetic resin film impregnated with or having embedded therein cholesterol oxidase, a system for the detection of hydrogen peroxide, buffer and at least one surfaceactive compound with lipophilic and hydrophilic properties; a p??cess for the activation of cholesterol oxidase is also provided.

PIPERIDINE DERIVATIVES OF INDOLE

New piperidine derivatives of indole and their process of preparation are provided in formula (I) (I) wherein R1 and R2, which may be the same or different, are selected from the group consisting of hydrogen atoms and lower alkyl radicals, R3 is a hydrogen or halogen atom or a lower alkyl or lower alkoxy radical and X is a straight-chain or branched alkylene radical containing 2 or 3 carbon atoms; and the acid addition salts thereof with pharmacologically compatible acids; the new derivatives and their pharmacologically compatible salts have an anti-hypertensive action.

INSTILLATION COMPOSITION

Instillation compositions are provided in which a sparingly soluble active material, for example, an antibacterial material, is suspended or partly dissolved in an aqueous emulsion which contains a swelling agent; the swelling agent stabilizes the emulsion containing the active material.

METHOD FOR IMMOBILIZING PROTEIN ON A CARRIER

Biologically-active proteins are immobilized by a method in which a compound containing at least two secondary amino groups is reacted at a temperature of from -80 to +50.degree.C. with a chlorination agent with the conversion of the secondary amino groups into imino-chloride groups of the general formula: R - ? = N - R1 in which R and R1, which can be the same or different, are aliphatic, aromatic or aliphatic-aromatic radicals, and the product thus obtained is either reacted in an aqueous solvent directly with a biologically-active protein, or is reacted with a bifunctional compound containing at least one primary amino group, optionally in an aqueous or organic solvent, and the product thereby obtained with groups of the general formula: in which R2 is an alkylene radical, an .alpha., .omega.-dioxyalkylene radical or a dicarboxylic acid amide group containing 2 to 8 carbon atoms and X is a primary amino group or a function capable of protein binding, is either reacted directly with a ...

ROTOR UNIT WITH INSERT ELEMENTS FOR A CENTRIFUGAL ANALYSER

The present invention provides a rotor unit for a centrifugal analyser with a rotor base connected with a drive and a rotor head which, in operation, is connected with the rotor base, said rotor head including chambers for the reception of a sample liquid and, radially outwardly from the associated sample chambers, measuring chambers for the measurement of characteristic parameters for the detection of components of the sample, as well as liquid channels for connecting the sample chambers with the measuring chambers, wherein the rotor head comprises a plurality of different insert elements which are exchangeable and connectable with the rotor base at different selectable positions, said insert elements being positionally stable when the centrifugal analyser is in operation. The present invention also provides an insert element for use with this rotor unit, wherein the insert element comprises a sample chamber, a measuring chamber and a fluid channel for connecting the sample chamber and ...

ROTOR UNIT WITH INSERT ELEMENTS FOR A CENTRIFUGAL ANALYSER

An insert element for use in a rotor unit of a centrifugal analyser for the analytical determincation of at least one component of a sample fluid comprises a housing containing at least one sample chamber for the reception of a sample liquid, at least one measuring chamber for the measurement of characteristic parameters for the detection of components of the sample, while the element rotates with the rotor unit, and a fluid connecting channel communicating each sample chamber with an opposed measuring chamber; the housing has retaining means adapted to cooperate with a rotor base of the rotor unit such that the housing is adapted to be held positionally stable on the rotor base when the centrifugal analyser is in operation, with the at least one measuring chamber disposed radially outwardly of the at least one sample chamber; at least one analysis reagent is disposed in the channel between the sample chamber and its opposed measuring chamber, such that in operation a sample liquid in a ...

PROCESS AND REAGENT FOR DETERMINATION OF GLYCEROL

The present invention provides a process for the determination of glycerol, wherein glycerol is incubated in an aqueous medium in the presence of oxygen with galactose oxidase and either the oxygen consumption or the hydrogen peroxide or glyceraldehyde formed are determined. The present invention also provides a reagent for the determination of glycerol, which comprises galactose oxidase and a system for the determination of hydrogen peroxide or a system for the determination of glyceraldehyde.

DERIVATIVES OF 4-PHENYLAMINOPYRIDINE, A PROCESS FOR THE PREPARATION THEREOF AND PHARMACEUTICAL COMPOSITIONS CONTAINING THEM

New derivatives of 4-phenylaminopyridine, a process for the preparation thereof and pharmaceutical compositions containing them The present invention provides derivatives of 4-phenylaminopyridine of the general formula: < IMG > (II) wherein X and Y are hydrogen atoms or hydroxyl groups, X being a hydroxyl group when Y is a hydrogen atom, whereas X is a hydrogen atom when Y is a hydroxyl group in one of the free positions of the aromatic nucleus and Z is a hydrogen atom or an alkyl radical containing up to 4 carbon atoms, which is optionally substituted by hydroxyl; and the pharmaceutically acceptable salts thereof. The present invention also provides a process for the preparation of these derivatives and pharmaceutical compositions containing them.

DIAGNOSTIC AGENT WITH A NEW STABILISER

... 1-Arylsemicarbazides are provided as stabilizers for oxygen indicators functioning as chromogen in diagnostic agents employed in the detection of glucose in body fluids, such as urine and blood; and the detection of blood in body materials such as urine, faeces and liquors; the stabilizers enable the manufacture of reliable test strips for rapid testing in medical diagnosis; the 1-arylsemicarbazides have the formula: Ar-NH-NH-CO-NH2 wherein Ar is an aryl radical which may be substituted by alkyl, alkoxy or halogen. ` ...

N-SUBSTITUTED CARBOXYLIC ACID AMIDES

An N-substituted carboxylic acid amide of the formula (1) wherein A is a valency bond or an oxygen or sulphur atom or a methylene, ethylene, vinylene, ethyleneoxa or ethylenethia radical, V is a trivalent hydrocarbon radical containing up to 4 carbon atoms, R1 and R2 each independently is hydrogen, a halogen atom, a hydroxyl group or an alkyl or alkoxy radical, R3 is hydrogen, a hydroxyl group or an alkoxy radical, Y is an alkylene radical containing up to 3 carbon atoms, X is a valency bond or a divalent aliphatic hydrocarbon radical containing up to 6 carbon atoms, and D is a hydroxymethyl, formyl, free or esterified car boxyl or an N-substituted carbamoyl radical, or a physiologically compatible salt thereof, characterized by hypoglycaemic and hypolipidaemic activity.

AMINOPROPANOL DERIVATIVES

The present invention provides aminopropanol derivatives of the general formula: (I) wherein R1 and R2, which can be the same or different, are hydrogen atoms or lower alkyl radicals or together represent an alkylene radical, R3 is a hydrogen atom or an acyl radical, A is one of the following structural elements: , , or in which R4 is a hydrogen atom or a lower alkyl radical, which is optionally substituted by hydroxyl, halogen, phenyl or alkylthio and R5 and R6, which can be the same or different, are lower alkyl radicals, and B is an alkylamino radical which optionally carries a phenyl and/or phenoxy radical, which can be substituted one or more times by halogen, hydroxyl, lower alkyl, lower acyl, lower alkylthio, acylamino, aminocarbonyl, lower alkoxy, lower alkenyloxy, phenoxy, lower alkenyl, lower alkylsulphonyl, lower alkylsulphinyl or haloalkyl, or is an an aryl- or heteroaryloxymethylpiperidine radical, which is optionally substituted onr or more times by ...

PROCESS AND AN AGENT FOR THE REMOVAL OF ASCORBIC ACID FROM AQUEOUS LIQUIDS

The present invention provides a process for removing ascorbic acid from aqueous solutions by the addition of ascorbate oxidase. wherein catalase and hydrogen peroxide are also added to the solution. The present invention also provides an agent for the removal of ascorbic acid from aqueous solutions, based on a solid carrier material impregnated with ascorbate oxidase, wherein the carrier material also contains catalase and hydrogen peroxide and optionally also a stabilizer and/or a buffer substance.

APPARATUS FOR DETERMINING THE DIFFUSE REFLECTIVITY OF A SAMPLE SURFACE OF SMALL DIMENSIONS

The present invention relates to an apparatus for determining the diffuse reflectivity of a sample surface of small dimensions, especially of the test field of a test strip for determining the substances contained in a body fluid. The apparatus has a semiconductor light emitter, especially a light-emitting diode, for the emission of visible or infrared light to the sample surface, a measuring receiver for receiving the light diffusely reflected by the sample surface and producing an electrical signal corresponding thereto, an evaluating means including an electronic circuit for converting the receiver signal to a value corresponding to the diffuse reflectivity, and a reference channel using the light from the same emitter for the calibration of the apparatus and for the elimination of errors of measurement caused by variations of the emitter or of other electronic components.

PROCESS AND REAGENT FOR THE DETERMINATION OF AN OXIDIZED PYRIDINE COENZYME

Process and reagent for the quantitative determination of an oxidised pyridine co-enzyme with oxidation with oxygen in the presence of the luciferase system and measurement of the emitted light, wherein an oxidised pyridine co-enzyme is reacted in the presence of its specific alcohol dehydrogenase with an aliphatic alcohol containing 8 to 16 carbon atoms and the aldehyde formed is oxidised with reduced flavine mononucleotide (FMNH2) and oxygen in the presence of luciferase and of the particular FMN reductase which is independent of the co-enzyme to be determined, as well as of the reduced substrate co-enzyme of the latter, with light emission the reagent comprises luciferase, FMN reductase, the aliphatic alcohol of 8 to 16 carbon atoms, the reduced co-enzyme, the alcohol dehydrogenase specific for the co-enzyme to be determined, the flavine-mononucleotide (FMN), a buffer and optionally an organic sulphhydryl compound and/or a surfaceactive agent.

ANILINE DERIVATIVES AND AGENTS FOR THE DETECTION OF REDOX SYSTEMS COMPRISING THEM

The present invention is concerned with the use of aniline derivatives of the general formula: (I) wherein R1 is a hydrogen atom or a lower alkyl or aryl radical, R2 is a hydrogen atom or a lower alkyl radical which can be substituted by hydroxyl, amino, carboxyl, lower alkoxycarbonyl, lower alkanoylamido, aryl lower alkyl or aryl or by a group of the structure: in which R5 and R6 have the meanings given hereinafter, R3 is a hydrogen or halogen atom or a carboxyl group, R4 and R'4, which can be the same or different, are hydrogen or halogen atoms, carboxyl groups or lower alkoxy radicals or lower alkyl radicals which are preferably in the m-position and R1 and R4 or R2 and R4 or R4 and R'4, when the two substituents are ortho to one another, can together form a saturated or unsaturated hydrocarbon chain containing 2 to 6 carbon atoms which can be substituted by hydroxyl or oxo groups, R5 is a hydrogen atom or a lower alkyl radical and R6 is a hydroxyl group or a lower alkoxy ...

DERIVATIVE OF TISSUE-TYPE PLASMINOGEN ACTIVATOR

A new tissue-type plasminogen activator (t-PA)-derivative is not glycosylated and consists of the following amino acid sequence: It is coded by the following DNA-sequence and possesses particularly favourable properties when used to dissolve blood clots.

COMPOSITIONS AND METHOD FOR DIAGNOSING DISEASES OF THE PANCREAS

PROCESS FOR PREPARING S-ADENOSYL HOMOCYSTEINE

PROCESS FOR PREPARING PARTICULATE MEDICINAL CARRIERS

3-NITROPYRAZOLE DERIVATIVES

OF THE DICLOSURE New 3-nitropyrazole derivatives and their process of preparation are provided of formula (I): (I) in which R1 is a lower alkyl radical, R2 is a hydrogen atom or a lower alkyl radical, R3 is a cyano, carboxyl or lower alkoxycarbonyl radical or the radical -CO-NR4R5, in which R4 is a hydrogen atom or a hydroxyl, carbamoyl, lower-alkylcarbamoyl, di-lower-alkylcarbamoyl, thiocarbamoyl, ureido, thioureido, hydroxyphenyl or picolyl radical or a lower alkyl, alkenyl or cyclo-lower-alkyl radical optionally substituted by a cyano, hydroxyl, pyrrolidino, piperidino, amino, lower alkylamino, lower dialkylamino or lower acylamino radical and R5 is a hydrogen atom or a lower alkyl radical or R4 and R5 together represent a lower alkylene bridge; and the pharmaceutically acceptable pharmacologically compatible salts thereof, the new derivatives have an anti-microbial action in vivo and particularly in the treatment of urinary infections.

2-HYDRAZONOPROPIONIC ACID DERIVATIVES AND THE PREPARATION THEREOF

New propionic acid derivatives and their process of preparation; the derivatives have the formula (I) (I) wherein R is a straight-chain, branched-chain or cyclic, saturated or unsaturated, aliphatic hydrocarbon radical and X is a valency bond or a straight-chain or branched-chain saturated or unsaturated, divalent aliphatic hydrocarbon radical containing up to 4 carbon atoms, with the proviso that when X is a valency bond, R is other than a saturated cycloalkyl radical: and the physiologically acceptable salts, esters and amide, thereof, and stereoisomers thereof: the new derivatives exhibit, in comparison with the corresponding hydrazines, a considerably increased hypoglycaemic action, whereas the MAO (monoamine oxidase) inhibiting action is almost completely suppressed.

PEROXIDASE DETERMINATION BY EMISSION OF LIGHT

The present invention provides a process for the determination of peroxidase in the presence of a compound, the enzyme-catalysed oxidation of which with a peroxy compound results in the emission of light, which is measured, wherein 7-dimethylaminonaphthalene-1,2-dicarboxylic acid hydrazide is used as light-emitting compound. The present invention also provides a reagent for the determination of peroxidase, comprising 7-dimethyl-aminonaphthalene-1,2-dicarboxylic acid hydrazide, a system providing hydrogen peroxide and a buffer substance of pH 6 to 9.

1-ACYL-2-CYANOAZIRIDINES AND THE PREPARATION THEREOF

New 1-acyl-2-cyanoaziridines are provided that are concerostatic and immune-stimulating therapeutic compounds that, while having the same or increased effectiveness and lower toxicity as compared with known 1-acyl-2cyanoaziridine derivatives can be administered orally; the derivatives when administered orally bring about marked leukocytosis with an immune-stimulating action and can, therefore, be used as immune-stimulating therapeutics.

AZIRIDINE DERIVATIVES

Novel aziridine derivatives and their process of preparation are provided of the formula (I): in which R is a nitrile or carbamoyl group and R1 and R2, which can be the same or different, are hydrogen atoms or aliphatic hydrocarbon radicals containing up to 10 carbon atoms, which can be straight-chanined, branched, cyclic, saturated or hydrocarbon and optionally substituted by hydroxyl, alkoxy, amino, alkylamino, dialkylamino, alkoxycarbonyl, cyano, 1, 2 or 3 halogens, cycloalkyl, phenyl or phenoxy- or axe nitrile, carboxyl, dialkylamino, or optionally hydrogenated monocyclic heteroaryl or phenyl radicals which a-a optionally substituted by alkyl, alkoxy, hydroxy, alkoxycarbonyl, dialkylamino, alkylthio, trifluoromethyl, nitro, carbamoyl, nitrile, sulphonamido, hydroxyalkyl or methylenedioxy groups or by halogen, or R1 and R2 together with the carbon atoms to which they are attached, can form a saturated or unsaturated ring containing up to 8 ring members, which can also be interrupted ...

BASIC-SUBSTITUTED DERIVATIVES OF 4-HYDROXY-BENZIMIDAZOLE

New basic-substituted derivatives of 4-hydroxy-benz-imidazole of the formula (I): ( I ) wherein R1 is a straight -chained or branched lower alkyl radical and R2 is a hydrogen atom or a lower alkyl radical; and the pharmacologically compatible, pharmaceutically acceptable acid addition salts thereof, bring about an inhibition of adrenergic .beta.-receptors and are, therefore, for the treatment of prophylaxis of cardiac and circulatory diseases.

HALOGENATED SULPHOPHTHALEINS

Octahalosulphophthaleins are provided of formula and the enol tautomer thereof wherein X is bromine or chlorine and each of X1, X2, X3 and X4 is a halogen atom selected from the group consisting of bromine and chlorine, provided at least one of X1, X2, X3 and X4 is different from X and provided that when X is chlorine at least one of X1, X2, X3 and X4 is chlorine; the novel compounds give a sensitive colour change in the presence of proteins and are useful in the detection of proteins in for example, body fluids.

RAPID DIAGNOSTIC FOR THE DETERMINATION OF URIC ACID IN BODY FLUIDS

The present invention provides a rapid diagnostic for the determination of uric acid in body fluids, using the principle of inversion colorimetry, wherein the indicator used is a radical of the general formula: in which R is a lower alkyl radical and Me is an alkali metal or an ammonium group.

PROCESS AND REAGENT FOR THE ENZYMATIC DETERMINATION OF ENZYME SUBSTRATES

The present invention provides a reagent for the enzymatic determination of an enzyme substrate, containing a system for the determination of the substrate, which comprises at least one enzyms, at least one buffer substance and at least one indicator substance and optionally contains adjuvants, wherein the reagent contains a definite amount of the enzyme substrate in an insufficient amount with regard to the substrate determination capacity of the system for the determination of this substrate. The present invention also provides a process for the enzymatic determination of an enzyme substrate in the presence of disturbing substance which can react with the substrate itself or with an intermediate or end product of the indicator reaction, wherein, before the addition of the sample to be investigated, a definite amount of the substrate is first reacted with the reagent until reaction is complete, whereafter the sample to be determined is added.

PROCESS AND REAGENT FOR DETERMINING TRIGLYCERIDES

For the determination of triglycerides by ester splitting with lipase and optionally esterase with the formation of fatty acids and glycerol, phosphorylation of the glycerol with ATP in the presence of glycerokinase with the formation of glycerol-1-phospate and ADP and determination of one of these formed substances, a glycerokinase from Bacillus stearothermophilis is combined with an activator selected from detergents, phenol derivatives and aniline derivatives; in addition, a suitable reagent contains lipase, ATP and glycerokinase from Bacillus stearothermophilis in combination with said activator and a system for determining glycerol-1-phospate or ADP, and optionally esterase; the activator is suitably a detergent, especially a polyethylene oxide ester, a polyethylene oxide ether, a bile acid or an alkyl sulphate.

DIAGNOSTIC AGENTS FOR THE DETECTION OF LEUKOCYTES IN BODY FLUIDS

The present invention provides a diagnostic agent for the detection of esterolytic and/or proteolytic enzymes, especially of leukocyte esterases and/or proteases, in body fluids, comprising a known esterase and/or protease substrate and conventional adjuvants, characterised in that it additionally contains at least one activator. The present invention also provides processes for the production of this diagnostic agent. Furthermore, the present invention is concerned with the use of this diagnostic agent for the detection of leukocytes in body fluids, particularly urine.

PROCESS AND A REAGENT FOR THE REMOVAL OF TURBIDITIES

For the removal of turbidities in reaction mixtures clouded by sample material, especially for photometric measurement, there are added to the reaction mixture:a) at least one low molecular weight organic compound, such as an aromatic alcohol or amine, which contains an electronegative substituent, for example a phenol or naphthol, which is substituted with one or more halogen atoms and/or hydroxyl groups andb) at least one detergent which dissolves without compound a) to give a clear solution and which forms a sparingly soluble complex with component a), such as a non-ionic polyethylene oxide adduct or a cationic detergent, especially an alkyl, aralkyl or alkylthio ether or an alkyl or aralkyl ester of a polyethylene oxide glycol containing 8 to 22 carbon atoms in the alkyl radical and 5 to 25 ethylene oxide units or an alkyl dimethyl benzyl ammonium chloride compound containing 8 to 22 carbon atoms in the alkyl radical, in an amount sufficient for the appearance of a precipitate of the ...

PROCESS AND REAGENT FOR THE DETERMINATION OF CHOLESTEROL

The present invention provides a process for the determination of cholesterol in free or found form by means of cholesterol oxidase and optionally of cholesterol esterase by measurement of the oxygen consumption, of the hydrogen peroxide formed or of the cholestenone formed, wherein the determination is carried out kinetically with the use of a cholesterol oxidase which has been obtained from a micro-organism of the genus Streptomyces and with the addition of 3,4-dichlorophenol. The present invention also provides a reagent for the kinetic determination of cholesterol, comprising cholesterol oxidase and a system for the determination of hydrogen peroxide or of cholestenone and optionally cholesterol esterase, wherein it additionally contains 3,4-dichlorophenol and the cholesterol oxidase used originates from a microorganism of the genus Streptomyces.

ANTI-ALLERGY BICYCLIC PHENOL ETHERS

Bicyclic phenol ethers of the formula (I): (I) wherein R1 is a hydrogen atom or a lower alkyl radical, R2 is a hydrogen atom or an acyl radical, X is an oxygen or sulphur atom or an -NH- group, Y is a -CO-CHR3- group or a -CO-C=CR4R5 group, R3 is a hydrogen atom, a lower alkyl radical, which can optionally be substituted by an aryl radical, or a cycloalkyl radical containing 3 to 7 carbon atoms and R4 and R5, which can be the same or different, are hydrogen atoms, lower alkyl radicals, alkenyl radicals containing 2 to 16 carbon atoms, which can optionally be substituted by an aryl radical, or an aryl radical or R4 and R5, together with the carbon atom to which they are attached, form a cycloalkyl ring containing 3 to 7 carbon atoms, and pharmaceutically acceptable, pharmacologically compatible salts thereof have an inhibiting action on anaphylactic reactions of the skin and of the bronchial system, even when administered orally in low dosages.

PYRROLOBENZIMIDAZOLES, PROCESS FOR THE PREPARATION THEREOF AND PHARMACEUTICAL COMPOSITIONS CONTAINING THEM

Pyrrolobenzimidazoles of the general formula: (I) wherein R1 is a hydrogen atom or an alkyl, alkenyl or cycloalkyl radical; R2 is a hydrogen atom, cyano group or alkyl or alkenyl radical or a carbonyl group substituted by hydroxyl, alkyl, alkoxy, amino, alkylamino, dialkylamino or hydrazino or together with R1 forms a cycloalkylene radical or R1 and R2 together form an alkylidene or cycloalkylidene radical; R3, R4 and R5, which can be the same or different, each represent a hydrogen atom or an alkanesulphonyloxy, trifluoromethanesulphonyloxy, alkanesulphonylamino, trifluoromethanesulphonylamino, N-alkyl-alkane-sulphonylamino, N-alkyl-trifluoromethanesulphonyl-amino, alkylsulphenylmethyl, alkylsulphinylmethyl or alkylsulphonylmethyl radical, a carbonyl group substituted by hydroxyl, alkoxy, amino, alkylamino or dialkylamino, a sulphonyl group substituted by amino, alkylamino, dialkylamino or cyclic imino, whereby a methylene group in the 4-position can be replaced by a sulphur or oxygen ...

PROCESS AND REAGENT FOR THE DETERMINATION OF THE LUTEINISING HORMONE AND MONOCLONAL ANTIBODIES SUITABLE THEREFOR

An immunological process for the determination of the luteinising hormone (LH) employs at least one monoclonal antibody which is directed against LH and cross-reacts with other glycoprotein hormones to an extent of less than 3%; a reagent for the determination of LH contains at least one such monoclonal antibody; monoclonal antibodies and a process and a hybridoma cell line for producing it are also provided.

GLYCOSIDES OF RESORUFIN DERIVATIVES, PROCESSES FOR THE PREPARATION THEREOF AND THE USE THEREOF FOR DETERMINING THE ACTIVITIES OF GLYCOSIDASES

Glycosides of resorufin derivatives, processes for the preparation thereof and the use thereof for determining the activities of glycosidases The present invention provides glycosides of resorufin derivatives of the general formulae: (Ia) (Ib) wherein R is a hydrogen atom, R2, R3 and R5, which can be the same or different, are hydrogen or halogen atoms or lower alkyl radicals, R4 and R6, which can be the same or different, are hydrogen or halogen atoms, cyano groups, lower alkyl, lower alkoxy, carboxyl, lower alkoxycarbonyl, carboxy lower alkyl or lower alkoxycarbonyl lower alkyl radicals or carboxamido groups optionally substituted once or twice or radicals of the general formula -COO-(CH2CH2O)n-R7, wherein R7 is a hydrogen atom or a lower alkyl radical and n is a whole number from 1 to 4, and R6 can additionally also represent a sulphonyl or nitro group and Y is a nitrogen atom or the group N ? 0. The present invention also provides processes for the preparation of these glycosides ...