01-05-2014 дата публикации
Номер: US20140120564A1
This invention provides devices, compositions and methods for determining the concentration of one or more metabolites or analytes in a biological sample, including cells, tissues, organs, organisms, and biological fluids. In particular, this invention provides materials, apparatuses, and methods for several non-invasive techniques for the determination of in vivo blood glucose concentration levels based upon the in vivo measurement of one or more biologically active molecules found in skin. 1. A sensor composition comprising at least one small molecule metabolic reporter (SMMR) , wherein the composition is applied to at least one surface of living tissue , organs , interstitial fluid , and whole organisms and transported into the tissue at an effective concentration , wherein when the at least one small molecule metabolic reporter is brought in contact with one or more specific metabolites , a change in fluorescence or absorption of the at least one small molecule metabolic reporter occurs , thereby allowing quantification of the change in fluorescence or absorption , thereby providing detailed in vivo information regarding picomolar through millimolar levels of cellular metabolites and metabolic precursors in the living tissue , organs , interstitial fluid , and whole organisms.2. The sensor composition of claim 1 , wherein the at least one small molecule metabolic reporter is selected from the group consisting of a fluorophore claim 1 , a protein labeled fluorophore claim 1 , a protein comprising a photooxidizable cofactor claim 1 , a protein comprising another intercalated fluorophore claim 1 , a mitochondrial vital stain or dye claim 1 , and a dye exhibiting at least one of a redox potential claim 1 , a membrane localizing dye claim 1 , a dye with energy transfer properties claim 1 , a pH indicating dye claim 1 , a coumarin dye claim 1 , a derivative of a coumarin dye claim 1 , an anthraquinone dye; a cyanine dye claim 1 , an azo dye; a xanthene dye; an ...
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