Thraustochytrids, fatty acid compositions, and methods of making and uses thereof

本发明涉及分离的破囊壶菌微生物以及其菌株和突变体。本发明还涉及生物质、微生物油、组合物、培养物、生成微生物油的方法、和使用分离破囊壶菌、生物质及微生物油的方法。

Thraustochytrids, Fatty Acid Compositions, and Methods of Making and Uses Thereof

The present invention is directed to isolated thraustochytrid microorganisms as well as strains and mutants thereof. The invention is further directed to biomasses, microbial oils, compositions, cultures, methods of producing microbial oils, and methods of using the isolated thraustochytrids, biomasses, and microbial oils.

Recombinant Thraustochytrids that Grow on Xylose, and Compositions, Methods of Making, and Uses Thereof

The present invention is directed to recombinant thraustochytrids that grow on xylose and cell cultures comprising the recombinant thraustochytrids as well as methods of producing cell cultures, biomasses, microbial oils, compositions, and biofuels using the recombinant thraustochytrids.

Thraustochytrids, fatty acid compositions, and methods of making and uses thereof

The present invention is directed to isolated thraustochytrid microorganisms as well as strains and mutants thereof. The invention is further directed to biomasses, microbial oils, compositions, cultures, methods of producing microbial oils, and methods of using the isolated thraustochytrids, biomasses, and microbial oils.

Schizochytrium PKS genes

The present invention relates to compositions and methods for preparing poly-unsaturated long chain fatty acids in plants, plant parts and plant cells, such as leaves, roots, fruits and seeds. Nucleic acid sequences and constructs encoding PKS-like genes required for the poly-unsaturated long chain fatty acid production, including the genes responsible for eicosapentenoic acid production of Shewanella putrefaciens and novel genes associated with the production of docosahexenoic acid in Vibrio marinus are used to generate transgenic plants, plant parts and cells which contain and express one or more transgenes encoding one or more of the PKS-like genes associated with such long chain polyunsaturated fatty acid production. Expression of the PKS-like genes in the plant system permits the large scale production of poly-unsaturated long chain fatty acids such as eicosapentenoic acid and docosahexenoic acid for modification of the fatty acid profile of plants, plant parts and tissues. Manipulation of the fatty acid profiles allows for the production of commercial quantities of novel plant oils and products.

Chimeric PUFA polyketide synthase systems and uses thereof

Disclosed are chimeric polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) proteins and chimeric PUFA PKS systems, including chimeric PUFA PKS proteins and systems derived from Schizochytrium and Thraustochytrium . Disclosed are nucleic acids and proteins encoding such chimeric PUFA PKS proteins and systems, genetically modified organisms comprising such chimeric PUFA PKS proteins and systems, and methods of making and using such chimeric PUFA PKS proteins and systems.

PUFA polyketide synthase systems and uses thereof

Disclosed are the complete polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems from the bacterial microorganisms Shewanella japonica and Shewanella olleyana , and biologically active fragments and homologues thereof. More particularly, this invention relates to nucleic acids encoding such PUFA PKS systems, to proteins and domains thereof that comprise such PUFA PKS systems, to genetically modified organisms (plants and microorganisms) comprising such PUFA PKS systems, and to methods of making and using the PUFA PKS systems disclosed herein. This invention also relates to genetically modified plants and microorganisms and methods to efficiently produce lipids enriched in various polyunsaturated fatty acids (PUFAs) as well as other bioactive molecules by manipulation of a PUFA polyketide synthase (PKS) system.

PUFA polyketide synthase systems and uses thereof

The invention generally relates to polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems, to homologues thereof, to isolated nucleic acid molecules and recombinant nucleic acid molecules encoding biologically active domains of such a PUFA PKS system, to genetically modified organisms comprising PUFA PKS systems, to methods of making and using such systems for the production of bioactive molecules of interest, and to novel methods for identifying new bacterial and non-bacterial microorganisms having such a PUFA PKS system.

Plant seed oils containing polyunsaturated fatty acids

Disclosed are plants that have been genetically modified to express a PKS-like system for the production of PUFAs (a PUFA PKS system), wherein oils produced by the plant contain at least one PUFA produced by the PUFA PKS system and are free of the mixed shorter-chain and less unsaturated PUFAs that are fatty acid products produced by the modification of products of the FAS system in standard fatty acid pathways. Also disclosed are the oil seeds, oils, and products comprising such oils produced by this system, as well as methods for producing such plants.

Nucleic acid molecule encoding ORFA of a PUFA polyketide synthase system and uses thereof

The invention generally relates to polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems isolated from or derived from Schizochytrium sp., to homologues thereof, to isolated nucleic acid molecules and recombinant nucleic acid molecules encoding biologically active domains of such a PUFA PKS system, to genetically modified organisms comprising PUFA PKS systems, to methods of making and using such systems for the production of bioactive molecules of interest, and to novel methods for identifying new bacterial and non-bacterial microorganisms having such a PUFA PKS system.

Production of DHA and other LC-PUFAs in plants

Oxylipins from stearidonic acid and γ-linolenic acid and methods of making and using the same

Disclosed are novel oxylipins that are derived from γ-linolenic acid (GLA; 18:3n-6) and stearidonic acid (STA or SDA; 18:4n-3), and methods of making and using such oxylipins. Also disclosed is the use of such oxylipins in therapeutic and nutritional or cosmetic applications, and particularly as anti-inflammatory or anti-neurodegenerative compounds. Also disclosed are The invention novel ways of producing long chain polyunsaturated acid (LCPUFA)-rich oils and compositions that contain enhanced and effective amounts of SDA- and/or GLA-derived oxylipins.

Enhanced production of lipids containing polyenoic fatty acids by high density cultures of eukaryotic microbes in fermentors

本发明提供了培养真核微生物的方法，所述微生物可以生产脂质，具体是含有多烯脂肪酸的脂质。本发明还提供了生产真核微生物脂质类的方法。

Enhanced production of lipids containing polyenoic fatty acids by very high density cultures of eukaryotic microbes in fermentors

本发明提供了培养真核微生物的方法，所述微生物可以生产脂质，具体是含有多烯脂肪酸的脂质。本发明还提供了生产真核微生物脂质类的方法。

Docosahexaenoic acid and compound including docosahexaenoic acid

【課題】ＤＨＡ含有食用単細胞オイルを提供する。 【解決手段】渦鞭毛虫、好ましくはクリプテコディニウムコニーを供給源とすることにより、トリグリセリドを含む食用単細胞オイルであって、ＤＨＡがオイルの少なくとも２０％を占める食用単細胞オイル、より好ましくは、少なくとも７０％のトリグリセリドを含み、ＤＨＡがトリグリセリドの少なくとも３０％を占める、食用単細胞オイルが提供される。 【選択図】図１

Process for growing thraustochytriales

Disclosed is a process for growing the microflora Thraustochytrium, Schizochytrium and mixtures thereof, which includes the growing of the microflora in fermentation medium containing non-chloride containing sodium salts, in particular sodium sulfate. In a preferred embodiment of the present invention, the process produces microflora having a cell aggregate size useful for the production of food products for use in aquaculture. Further disclosed is a food product which includes Thraustochytrium, Schizochytrium, and mixtures thereof, and a component selected from flaxseed, rapeseed, soybean and avocado meal. Such a food product includes a balance of long chain and short chain omega-3 highly unsaturated fatty acids.

Solventless extraction process

The present invention provides a method for extracting lipids from microorganisms without using a nonpolar organic solvent as an extraction solvent. In particular, the present invention provides a method for extracting lipids from microorganisms by lysing cells and removing water soluble compound and/or materials by washing the lysed cell mixtures with aqueous washing solutions until a substantially non-emulsified lipid is obtained.

Process for the heterotrophic production of products with high concentrations of omega-3 highly unsaturated fatty acids

A process for the heterotrophic or predominantly heterotrophic production of whole-celled or extracted microbial products with a high concentration of omega-3 highly unsaturated fatty acids, producible in an aerobic culture under controlled conditions using biologically pure cultures of heterotrophic single-celled fungi microorganisms of the order Thraustochytriales. The harvested whole-cell microbial product can be added to processed foods as a nutritional supplement, or to fish and animal feeds to enhance the omega-3 highly unsaturated fatty acid content of products produced from these animals. The lipids containing these fatty acids can also be extracted and used in nutritional, pharmaceutical and industrial applications.

DHA Triglyceride Emulsions

The present invention is directed to an emulsion comprising an emulsifier, an isotonic agent and a docosahexaenoic acid triglyceride (DHA-TG) wherein the emulsion is substantially free of eicosapentaenoic acid (EPA) and is suitable for parenteral administration.

Method for production of arachidonic acid

【課題】アラキドン酸を経済的に生成し、それを配合した食品を提供する。 【解決手段】好ましくは複合窒素源成分を含有する発酵培地において、モルティエラ シュマッカリ亜属の微生物を培養する。アラキドン酸は幼児の栄養素として重要なエイコサノイドの前駆体であり、食品にモルティエラ シュマッカリ亜属の微生物、又はこれらの微生物から分離される脂質を配合して、アラキドン酸含量を向上させた幼児用調合乳やベビーフードが製造される。 【選択図】なし

Production of DHA in microalgae in low pH medium

Methods for production of highly unsaturated fatty acids by marine microorganisms, including the heterotrophic marine dinoflagellate Crypthecodinium , using low levels of chloride ion are disclosed. Specifically, methods of increasing production of highly unsaturated fatty acids by marine microorganisms while growing in low chloride media by manipulating sodium ion and potassium ion levels. The invention also relates to methods of production of highly unsaturated fatty acids by marine organisms at low pH levels, and includes methods for generation of low pH tolerant strains.

PUFA polyketide synthase systems and uses thereof

Disclosed are the complete polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems from the bacterial microorganisms Shewanella japonica and Shewanella olleyana , and biologically active fragments and homologues thereof. More particularly, this invention relates to nucleic acids encoding such PUFA PKS systems, to proteins and domains thereof that comprise such PUFA PKS systems, to genetically modified organisms (plants and microorganisms) comprising such PUFA PKS systems, and to methods of making and using the PUFA PKS systems disclosed herein. This invention also relates to genetically modified plants and microorganisms and methods to efficiently produce lipids enriched in various polyunsaturated fatty acids (PUFAs) as well as other bioactive molecules by manipulation of a PUFA polyketide synthase (PKS) system.

Solvent-free extraction process

Foreliggende oppfinnelse tilveiebringer lipider ekstrahert fra mikroorganismer uten 5 anvendelse av et ikke-polart organisk løsningsmiddel som ekstraksjonsløsningsmiddel. Spesielt tilveiebringer foreliggende oppfinnelse lipider ekstrahert fra mikroorganismer ved lysing av celler og fjerning av vannoppløselige forbindelser og/eller materialer ved vasking av de lysede celle-blandinger med vandige vaskeløsninger inntil et hovedsakelig ikke-emulgert lipid blir oppnådd.

Product and process for transformation of thraustochytriales microorganisms

本发明公开了均来自破囊壶菌目微生物的乙酰乳酸合酶，乙酰乳酸合酶调控区，α－微管蛋白启动子，来自破囊壶菌目聚酮化合物合酶(PKS)系统的启动子，和脂肪酸脱氢酶启动子的核酸和氨基酸序列。本发明也公开了用于破囊壶菌目微生物转化的重组载体，和转化破囊壶菌目微生物的方法。所述本发明的重组核酸分子可被用于外源核酸在破囊壶菌目微生物的表达，和基因在破囊壶菌目微生物的去除，突变或灭活。

Process for obtaining lipid from cells

The present invention relates to a process for obtaining lipid from a composition comprising cells and water, said process comprising contacting the composition with a desiccant, and recovering the lipid from the cells. The invention also relates to a lipid obtainable by this process. The process according to then invention enables lipid of high quality to be obtained with a high yield, and does not require a heating step.

Method for the separation of phospholipids from phospholipid-containing materials

Enhanced production of lipids containing polyenoic fatty acids by high density cultures of eukaryotic microbes in fermentors

The present invention provides a process for growing eukaryotic microorganisms, which are capable of producing lipids, in particular, lipids containing polyenoic fatty acids. The present invention also provides a process for producing eukaryotic microbial lipids.

Product and process for transformation of thraustochytriales microorganisms

Disclosed are nucleic acid and amino acid sequences for acetolactate synthase, acetolactate synthase regulatory regions, α-tubulin promoter, a promoter from a Thraustochytriales polyketide synthase (PKS) system, and fatty acid desaturase promoter, each from a Thraustochytriales microorganism. Also disclosed are recombinant vectors useful for transformation of Thraustochytriales microorganisms, as well as a method of transformation of Thraustochytriales microorganisms. The recombinant nucleic acid molecules of the present invention can be used for the expression of foreign nucleic acids in a Thraustochytriales microorganism as well as for the deletion, mutation, or inactivation of genes in Thraustochytriales microorganisms.

Enhanced production of lipids containing polyenoic fatty acids by high density cultures of eukaryotic microbes in fermentors

本发明涉及通过在发酵罐中高密度培养真核微生物来增加含有多烯脂肪酸的脂质的产生。本发明提供了培养真核微生物的方法，所述微生物可以生产脂质，具体是含有多烯脂肪酸的脂质。本发明还提供了生产真核微生物脂质类的方法。

Process for the Heterotrophic Production of Microbial Products with High Concentrations of Omega-3 Highly Unsaturated Fatty Acids

Disclosed is a process for growing the microflora Thraustochytrium, Schizochytrium and mixtures thereof, which includes the growing of the microflora in fermentation medium containing non-chloride containing sodium salts, in particular sodium sulfate. In a preferred embodiment of the present invention, the process produces microflora having a cell aggregate size useful for the production of food products for use in aquaculture. Further disclosed is a food product which includes Thraustochytrium, Schizochytrium and mixtures thereof, and a component selected from flaxseed, rapeseed, soybean and avocado meal. Such a food product includes a balance of long chain and short chain omega-3 highly unsaturated fatty acids.

Pufa polyketide synthase systems and uses thereof

Disclosed are the complete polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems from the bacterial microorganisms Shewanella japonica and Shewanella olleyana , and biologically active fragments and homologues thereof. More particularly, this invention relates to nucleic acids encoding such PUFA PKS systems, to proteins and domains thereof that comprise such PUFA PKS systems, to genetically modified organisms (plants and microorganisms) comprising such PUFA PKS systems, and to methods of making and using the PUFA PKS systems disclosed herein. This invention also relates to genetically modified plants and microorganisms and methods to efficiently produce lipids enriched in various polyunsaturated fatty acids (PUFAs) as well as other bioactive molecules by manipulation of a PUFA polyketide synthase (PKS) system.

Carotene synthase gene and uses therefor

Described herein is a novel three domain gene from Schizochytrium, denoted carotene synthase, that encodes a protein with three different enzymatic activities; phytoene dehydrogenase (PD), phytoene synthase (PS), and lycopene cyclase (LC). Also described is the isolated gene encoding the carotene synthase, homologues thereof, the enzyme encoded by such gene, biologically active portions and homologues thereof and fusion proteins comprising said enzyme portions and homologues, recombinant nucleic acid molecules, microorganisms and plants that have been genetically modified to increase or decrease the action of such gene, and methods of producing carotenoids and derivatives thereof. Also described are methods of producing microorganisms and products comprising lipids lacking pigmentation using the knowledge of the carotene synthase described herein and products of said methods.

Methods of Incorporating Polyunsaturated Fatty Acids in Milk

Method for incorporating polyunsaturated fatty acids into milk with improved efficiency. The methods include protecting the polyunsaturated fatty acids, including omega-3 and omega-6 polyunsaturated fatty acids, with a protective agent prior to feeding the fatty acids to a milk producing animal. Methods for feeding polyunsaturated fatty acids to milk producing animals by top dressing a polyunsaturated fatty acid supplement on top of animal feed compositions and methods of making and using such compositions are also provided.

Docosahexaenoic Acid Gel Caps

The present invention is directed to an oral dosage form comprising a gel capsule comprising, (a) plasticizer; (b) gelatin; (c) water; and (d) greater than 850 mg DHA ethyl ester, wherein less than about 2% (wt/wt) of the total fatty acid content of the dosage form is EPA, wherein the total weight of the capsule is about 1 g and wherein the capsule is stable at about 25° C. at 60% relative humidity.

PUFA polyketide synthase systems and uses thereof

The invention generally relates to polyunsaturated fatty acid (PUFA) polyketide synthase (PKA) systems isolated from or derived from non-bacterial organisms, to homologues thereof, to isolated nucleic acid molecules and recombinant nucleic acid molecules encoding biologically active domains of such a PUFA PKS system, to methods of making and using such system for the production of bioactive molecules of interest, and to novel methods for identifying new bacterial and non-bacterial microorganisms having such a PUFA PKS system.

Product and process for transformation of thraustochytriales microorganisms

Microwaveable Popcorn and Methods of Making

Microwaveable popcorn compositions, products and methods of making the same are disclosed. The composition includes unpopped popcorn kernels, an oil comprising at least one omega-3 LC PUFA, and an oil that is solid at about 20° C. The an oil comprising at least one omega-3 LC PUFA can include a microbial oil prepared by extraction with isopropanol and water in the absence of winterization.

PROCESS TO OBTAIN A LIPID FROM CELLS.

Un proceso para obtener un lípido a partir de una composición que comprende células y agua, donde dicho proceso comprende: (a) poner en contacto la composición con un desecante; y (b) recuperar el lípido de las células. A process for obtaining a lipid from a composition comprising cells and water, wherein said process comprises: (a) contacting the composition with a desiccant; and (b) recover the lipid from the cells.

Methods for controlling highly unsaturated fatty acid content in various tissues

A method of treating a neurological disorder comprises administering to a person affected from such a disorder a microbial oil comprising DHA, a microbial oil comprising ARA or a combination of DHA and ARA oils in an amount sufficient to elevate the levels of circulating DHA and/or ARA in the person's blood to at least normal levels.

Use of docosahexaenoic acid in treating mild cognitive impairment

The present invention is directed to methods of improving cognitive function in subjects having age related cogni-tive decline or mild cognitive impairment and to methods of decreasing heart rate in a subject by administering dosage forms com-prising docosahexaenoic acid (DHA) substantially free of eicosapentaenoic acid (EPA).

Process for obtaining lipid from cells

The present invention relates to a process for obtaining lipid from a composition comprising cells and water, said process comprising contacting the composition with a desiccant, and recovering the lipid from the cells. The invention also relates to a lipid obtainable by this process. The process according to then invention enables lipid of high quality to be obtained with a high yield, and does not require a heating step.

Polyunsaturated fatty acid production in heterologous organisms using PUFA polyketide synthase systems

Schizochytrium PKS genes

The present invention relates to compositions and methods for preparing poly-unsaturated long chain fatty acids in plants, plant parts and plant cells, such as leaves, roots, fruits and seeds. Nucleic acid sequences and constructs encoding PKS-like genes required for the poly-unsaturated long chain fatty acid production, including the genes responsible for eicosapentenoic acid production of Shewanella putrefaciens and novel genes associated with the production of docosahexenoic acid in Vibrio marinus are used to generate transgenic plants, plant parts and cells which contain and express one or more transgenes encoding one or more of the PKS-like genes associated with such long chain polyunsaturated fatty acid production. Expression of the PKS-like genes in the plant system permits the large scale production of poly-unsaturated long chain fatty acids such as eicosapentenoic acid and docosahexenoic acid for modification of the fatty acid profile of plants, plant parts and tissues. Manipulation of the fatty acid profiles allows for the production of commercial quantities of novel plant oils and products.

Production of DHA in Microalgae in Low pH Medium

Methods for production of highly unsaturated fatty acids by marine microorganisms, including the heterotrophic marine dinoflagellate Crypthecodinium , using low levels of chloride ion are disclosed. Specifically, methods of increasing production of highly unsaturated fatty acids by marine microorganisms while growing in low chloride media by manipulating sodium ion and potassium ion levels. The invention also relates to methods of production of highly unsaturated fatty acids by marine organisms at low pH levels, and includes methods for generation of low pH tolerant strains.

PUFA polyketide synthase systems and uses thereof

The invention generally relates to polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems isolated from or derived from non-bacterial organisms, to homologues thereof, to isolated nucleic acid molecules and recombinant nucleic acid molecules encoding biologically active domains of such a PUFA PKS system, to genetically modified organisms comprising PUFA PKS systems, to methods of making and using such systems for the production of bioactive molecules of interest, and to novel methods for identifying new bacterial and non-bacterial microorganisms having such a PUFA PKS system.

Arachidonic acid and process for its preparation and use thereof

Process for increasing the amount of omega-3-high unsaturated fatty acids in peat meats, as well as food product.

Dha-containing nutritional compositions and methods for their production

Extraction of Lipid From Cells and Products Therefrom

The present invention relates to processes for obtaining a lipid from a cell by lysing the cell, contacting the cell with a base and/or salt, and separating the lipid. The present invention is also directed to a lipid prepared by the processes of the present invention. The present invention is also directed to microbial lipids having a particular anisidine value, peroxide value, and/or phosphorus content.

Arachidonic acid and processes for its preparation and its use

Isolated nucleic acid molecule comprising a nucleic acid sequence encoding an acyl-coa synthetase, an isolated recombinant host cell comprising same and method to produce oil

Polyunsaturated fatty acid-containing solid fat compositions and uses and production thereof

The present invention provides a solid fat composition that includes an oil having saturated fat and an oil having at least one long chain polyunsaturated fatty acid. In particular, the solid fat composition can have high levels of long chain polyunsaturated fatty acid and low to no presence of emulsifiers. In preferred embodiments, the polyunsaturated oil is an unwinterized microbial oil. The invention also relates to methods for making such compositions and food, nutritional, and pharmaceutical products comprising said compositions.

Trophic conversion of obligate phototrophic algae through metabolic engineering

Most microalgae are obligate photoautotrophs and their growth is strictly dependent on the generation of photosynthetically-derived energy. In this study it is shown that the microalga Phaeodaclylurn tricornutum can be engineered to import glucose and grow in the dark through the introduction of genes encoding glucose transporters. Both the human and Chlorella kessleri glucose transporters facilitated the uptake of glucose by P. tricornutum , allowing the cells to metabolize exogenous organic carbon and thrive, independent of light. This is the first successful trophic conversion of an obligate photoautotroph through metabolic engineering, and it demonstrates that methods of cell nourishment can be fundamentally altered with the introduction of a single gene. Since strains transformed with the glucose transport genes are able to grow non-photosynthetically, they can be exploited for the analysis of photosynthetic processes through mutant generation and characterization. Finally, this work also represents critical progress toward large-scale commercial exploitation of obligate phototrophic algae through the use of microbial fermentation technology, eliminating significant limitations resulting from light-dependent growth.

Food fortification with polyunsaturated fatty acids

Coated food products fortified with a polyunsaturated fatty acid, including sweetened food products, and methods for their preparation are provided.

Methods for improving cognitive function and decreasing heart rate

The present invention is directed to methods of improving cognitive function in subjects having age related cognitive decline or mild cognitive impairment and to methods of decreasing heart rate in a subject by administering dosage forms comprising docosahexaenoic acid (DHA) substantially free of eicosapentaenoic acid (EPA).

METHODS AND UTILIZING PHARMACEUTICAL COMPOSITIONS IN THE TREATMENT OF NEUROLOGICAL DISTURBLES

Polyunsaturated fatty acids for treatment of dementia and pre-dementia-related conditions

Disclosed are compositions and methods for treating or preventing dementia and pre-dementia-related conditions and/or symptoms or characteristics of such conditions.

PUFA polyketide synthase systems and uses thereof

The invention generally relates to polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems isolated from or derived from non-bacterial organisms, to homologues thereof, to isolated nucleic acid molecules and recombinant nucleic acid molecules encoding biologically active domains of such a PUFA PKS system, to genetically modified organisms comprising PUFA PKS systems, to methods of making and using such systems for the production of bioactive molecules of interest, and to novel methods for identifying new bacterial and non-bacterial microorganisms having such a PUFA PKS system.

Dha-containing nutritional compositions and methods for their production

Polyunsaturated fatty acid-containing solid fat compositions and uses and production thereof

本发明提供了一种固体脂肪组合物，其包含具有饱和脂肪的油和具有至少一种长链多不饱和脂肪酸的油。具体说，所述固体脂肪组合物具有高水平的长链多不饱和脂肪酸和低水平的乳化剂或不含乳化剂。在优选实施方式中，所述多不饱和油是未经冬化处理的微生物油。本发明还涉及制备这些组合物以及包含所述组合物的食品、营养品和药品的方法。

A method of improving the flavor, tenderness and overall consumer acceptability of poultry meat.

PUFA polyketide synthase systems and uses thereof

The invention generally relates to polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems, to homologues thereof, to isolated nucleic acid molecules and recombinant nucleic acid molecules encoding biologically active domains of such a PUFA PKS system, to genetically modified organisms comprising PUFA PKS systems, to methods of making and using such systems for the production of bioactive molecules of interest, and to novel methods for identifying new bacterial and non-bacterial microorganisms having such a PUFA PKS system.

Pufa polyketide synthase systems and uses thereof

The invention generally relates to polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems, to homologues thereof, to isolated nucleic acid molecules and recombinant nucleic acid molecules encoding biologically active domains of such a PUFA PKS system, to genetically modified organisms comprising PUFA PKS systems, to methods of making and using such systems for the production of bioactive molecules of interest, and to novel methods for identifying new bacterial and non-bacterial microorganisms having such a PUFA PKS system.

PUFA polyketide synthase systems and uses thereof

Disclosed are the complete polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems from the bacterial microorganisms Shewanella japonica and Shewanella olleyana , and biologically active fragments and homologues thereof. More particularly, this invention relates to nucleic acids encoding such PUFA PKS systems, to proteins and domains thereof that comprise such PUFA PKS systems, to genetically modified organisms (plants and microorganisms) comprising such PUFA PKS systems, and to methods of making and using the PUFA PKS systems disclosed herein. This invention also relates to genetically modified plants and microorganisms and methods to efficiently produce lipids enriched in various polyunsaturated fatty acids (PUFAs) as well as other bioactive molecules by manipulation of a PUFA polyketide synthase (PKS) system.

Promotor, acetolactate synthase and process for transformation of thraustochytriales microorganisms

Disclosed are nucleic acid and amino acid sequences for acetolactate synthase, acetolactate synthase regulatory regions, α-tubulin promoter, a promoter from a Thraustochytriales polyketide synthase (PKS) system, and fatty acid desaturase promoter, each from a Thraustochytriales microorganism. Also disclosed are recombinant vectors useful for transformation of Thraustochytriales microorganims, as well as a method of transformation of Thraustochytriales microorganisms. The recombinant nucleic acid molecules of the present invention can be used for the expression of foreign nucleic acids in a Thraustochytriales microorganism as well as for the deletion, mutation, or inactivation of genes in Thraustochytriales microorganisms.

Process for preparing materials for extraction

The present invention relates to a process for preparing a biomass, such as from a microbial fermentation, for an extraction process to separate desired chemicals, nutritional products, bioactive components, proteins, carbohydrates, and lipids, from the biomass. Particularly preferred substances to extract include docosahexaenoic acid, docosapentaenoic acid, and arachidonic acid. The present invention also includes extracting the prepared biomass. Biomasses to be treated in accordance with the methods of the invention include plant, animal, and microbial biomass, particularly a microorganism such as Crypthecodinium cohnii and a fungus such as Mortierella alpina.

Extraction and winterization of lipids from oilseed and microbial sources

本发明公开了主要具有含有至少一种长链多不饱和脂肪酸的中性脂类成分的脂类组合物的纯化方法。该方法使用使所述的脂类组合物接触极性溶剂、诸如丙酮接触的步骤，其中对所述的溶剂进行选择，使得溶于该溶剂的污染物比溶于所述长链多不饱和脂肪酸的少。该方法一般在冷却温度、包括约0℃下进行。在从所述脂类组合物中沉淀污染物时，进行分离以便从该脂类组合物中除去沉淀的物质。所述的长链多不饱和脂肪酸可以包括ARA、DPA、EPA和/或DHA。该方法可以有效使脂类组合物冬化，由此减少这类组合物变浑浊的倾向。

USEFUL METHODS AND PHARMACEUTICAL COMPOSITIONS FOR THE TREATMENT OF NEUROLOGICAL DISEASES

Enhanced production of lipids containing polyenoic fatty acid by very hugh density cultures of eukaryotic microbes in fermentors

The present invention provides a process for growing eukaryotic microorganisms which are capable of producing lipids, in particular lipids containing polyenoic fatty acids. The present invention also provides a process for producing eukaryotic microbial lipids.

Solventless extraction process

The present invention provides a method for extracting lipids from microorganisms without using a nonpolar organic solvent as an extraction solvent. In particular, the present invention provides a method for extracting lipids from microorganisms by lysing cells and removing water soluble compound and/or materials by washing the lysed cell mixtures with aqueous washing solutions until a substantially non-emulsified lipid is obtained.

EXTRACTION OF CELL LIPIDS AND PRODUCTS FROM THEM

La presente se refiere a procesos para obtener un lípido de una célula por lisado de la célula, la puesta en contacto de la célula con una base y/o la sal y la separación del lípido. La presente también se refiere a un lípido preparado por medio de los procesos de la presente. La presente también se refiere un lípido microbiano que tiene un valor de anisidina particular, valor de peróxido y/o contenido de fósforo.Reivindicación 1: Un proceso para obtener un lípido de una composición celular microbiana, que comprende: elevar el pH de la composición celular a 8 o más; y separar un lípido de la composición celular, en donde el lípido contiene menos del 5% en peso de un solvente orgánico. This refers to processes for obtaining a lipid from a cell by lysing the cell, bringing the cell into contact with a base and / or salt and separating the lipid. The present also refers to a lipid prepared by means of the processes of the present. The present invention also refers to a microbial lipid having a particular anisidine value, peroxide value and / or phosphorus content. Claim 1: A process for obtaining a lipid of a microbial cellular composition, comprising: raising the pH of the composition cell phone at 8 or more; and separating a lipid from the cellular composition, wherein the lipid contains less than 5% by weight of an organic solvent.

Enhanced production of lipids containing polyenoic fatty acid by very high density cultures of eukaryotic microbes in fermentors

The present invention provides a process for growing eukaryotic microorganisms which are capable of producing lipids, in particular lipids containing polyenoic fatty acids. The present invention also provides a process for producing eukaryotic microbial lipids.

1C, 15N, 2H labeled proteins for NMR structural determinations and their preparation

Method and kit for making a multidimensional combinatorial chemical library

Building blocks for making a combinatorial chemical library comprise α-allyl carboxylic acids and their functionalized derivatives. These are covalently linked by monotonous or diverse linkages. These can be conformationally constrained by cyclization and annelation. Kits comprising diverse α-allyl carboxylic acids can be used to make libraries.

Process for separation of renewable materials from microorganisms

Methods of separating renewable materials, such as lipids, from microorganisms, such as oleaginous yeasts, may include conditioning cell walls of the microorganisms to form, open or enlarge pores, and removing at least a portion of the renewable material through the pores. These methods may result in delipidated microorganisms with cell walls that are substantially intact and with mesopores. These delipidated microorganisms may be used to produce biofuels.

Extraction and winterization of lipids from oilseed and microbial sources

A process for purifying a lipid composition having predominantly neutral lipid components having at least one long chain polyunsaturated fatty acid is disclosed. The process employs contacting the lipid composition with polar solvent, such as acetone, wherein the solvent is selected such that contaminants are less soluble in the solvent than in the long chain polyunsaturated fatty acid. The process is typically conducted at cooler temperatures, including about 0°C. Upon precipitation of the contaminants from the lipid composition, a separation is conducted to remove the precipitated material from the lipid composition. The long chain polyunsaturated fatty acids can include ARA, DPA, EPA and/or DHA. The process effectively winterizes lipid compositions, thereby reducing the tendency of such compositions to become hazy.

High fluorescent intensity cross-linked allophycocyanin

Solventless extraction process

The present invention provides a method for extracting lipids from microorganisms without using organic solvent as an extraction solvent. In particular, the present invention provides a method for extracting lipids from microorganisms by lysing cells and removing water soluble compound and/or materials by washing the lysed cell mixtures with aqueous washing solutions until a substantially non-emulsified lipid is obtained.

PUFA polyketide synthase systems and uses thereof

The invention generally relates to polyunsaturated fatty acid (PUFA) polyketide synthase (PKA) systems isolated from or derived from non-bacterial organisms, to homologues thereof, to isolated nucleic acid molecules and recombinant nucleic acid molecules encoding biologically active domains of such a PUFA PKS system, to methods of making and using such system for the production of bioactive molecules of interest, and to novel methods for identifying new bacterial and non-bacterial microorganisms having such a PUFA PKS system.

Enriched Beverages and Methods of Making The Same

The present invention provides a composition that contains a beverage component, pectin and an oil at least one long chain polyunsaturated fatty acid and a method for making the same.

Procedure for the heterotrophic production of microbial products with large concentrations of very unsaturated omega-3 fatty acids

SE DESCRIBE UN PROCESO PARA EL CULTIVO DE MICROFLORA DE THRAUSTOCHITRIUM, SCHIZOCHITRIUM Y MEZCLAS DE LAS MISMAS, QUE INCLUYE EL CRECIMIENTO DE LA MICROFLORA EN UN MEDIO DE FERMENTACION QUE CONTIENE SALES DE SODIO QUE NO TIENEN CLORO, EN PARTICULAR SULFATO DE SODIO. EN UNA VERSION REFERIDA DEL PRESENTE INVENTO, EL PROCESO PRODUCE MICROFLORA QUE TIENE UN TAMAÑO DE AGREGACION CELULAR UTIL PARA LA PRODUCCION DE PRODUCTOS ALIMENTICIOS PARA EMPLEAR EN AGRICULTURA. ADEMAS SE DESCRIBE UN PRODUCTO ALIMENTICIO QUE INCLUYE THRAUSTOCHITRIUM, SCHIZOCHITRIUM Y MEZCLAS DE LAS MISMAS, Y UN COMPONENTE SELECCIONADO ENTRE LINAZA, SEMILLAS DE COLZA, SOJA Y HARINA DE AGUACATE. TAL PRODUCTO ALIMENTICIO INCLUYE UN EQUILIBRIO DE ACIDOS GRASOS ALTAMENTE INSATURADOS OMEGA - 3 DE CADENA CORTA Y CADENA LARGA. A PROCESS FOR THE MICROFLORA CULTURE OF THRAUSTOCHITRIUM, SCHIZOCHITRIUM AND MIXTURES OF THE SAME IS DESCRIBED, INCLUDING THE GROWTH OF THE MICROFLORA IN A FERMENTATION MEDIA CONTAINING SODIUM SALTS THAT DO NOT HAVE CHLORINE, IN PARTICULAR OF SATO. IN A REFERRED VERSION OF THIS INVENTION, THE PROCESS PRODUCES MICROFLORA THAT HAS A USEFUL CELLULAR AGGREGATION SIZE FOR THE PRODUCTION OF FOOD PRODUCTS TO BE USED IN AGRICULTURE. IN ADDITION A FOOD PRODUCT IS DESCRIBED THAT INCLUDES THRAUSTOCHITRIUM, SCHIZOCHITRIUM AND MIXTURES OF THE SAME, AND A SELECTED COMPONENT BETWEEN LINAZA, COLZA SEEDS, SOY AND AVOCADO FLOUR. SUCH FOOD PRODUCT INCLUDES A BALANCE OF HIGHLY INSATURED FATTY ACIDS OMEGA - 3 OF SHORT CHAIN AND LONG CHAIN.

Process for obtaining lipid from cells

The present invention relates to a process for obtaining lipid from a composition comprising cells and water, said process comprising contacting the composition with a desiccant, and recovering the lipid from the cells. The invention also relates to a lipid obtainable by this process. The process according to then invention enables lipid of high quality to be obtained with a high yield, and does not require a heating step.

Process for the preparation of arachidonic acid

Infant formulas and other food products containing phospholipids

A food product containing mixed glycerides, including polyunsaturated fatty acid residues, highly unsaturated fatty acid residues, or a combination thereof, is stabilized by the addition of phospholipids. The addition of phospholipids to fat blends has been found to have an unexpected stabilizing effect on the fats contained in the blend. The phospholipids are preferably derived from vegetables, milk, microbes, or a combination thereof.

Pufa polyketide synthase systems and uses thereof

The invention generally relates to polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems isolated from or derived from non-bacterial organisms, to homologues thereof, to isolated nucleic acid molecules and recombinant nucleic acid molecules encoding biologically active domains of such a PUFA PKS system, to genetically modified organisms comprising PUFA PKS systems, to methods of making and using such systems for the production of bioactive molecules of interest, and to novel methods for identifying new bacterial and non-bacterial microorganisms having such a PUFA PKS system.

ARACHIDON ACID AND METHOD FOR THE PRODUCTION AND USE THEREOF

Processed Meat Products and Methods of Making

Processed meat products and methods of making the same are disclosed. The methods include incorporating a powder-like substance comprising an oil comprising a long chain polyunsaturated fatty acid into a processed meat product.

Extraction and winterization of lipids from oilseed and microbial sources

A process for purifying a lipid composition having predominantly neutral lipid components having at least one long chain polyunsaturated fatty acid is disclosed. The process employs contacting the lipid composition with a polar solvent, such as acetone, wherein the solvent is selected such that the contaminants are less soluble in the solvent than in the long chain polyunsaturated fatty acid. The process is typically conducted at cooler temperatures including about 0 °C. Upon precipitation of the contaminants from the lipid composition, a separation is conducted to remove the precipitated material from the lipid composition. The long chain polyunsaturated fatty acids can include ARA, DPA, EPA and/or DHA. The process effectively winterizes lipid compositions, thereby reducing the tendency of such compositions to become hazy.

Extraction and winterization of lipids from oilseed and microbial sources

A process for purifying a lipid composition having predominantly neutral lipid components having at least one long chain polyunsaturated fatty acid is disclosed. The process employs contacting the lipid composition with a polar solvent, such as acetone, wherein the solvent is selected such that the contaminants are less soluble in the solvent than in the long chain polyunsaturated fatty acid. The process is typically conducted at cooler temperatures including about 0° C. Upon precipitation of the contaminants from the lipid composition, a separation is conducted to remove the precipitated material from the lipid composition. The long chain polyunsaturated fatty acids can include ARA, DPA, EPA and/or DHA. The process effectively winterizes lipid compositions, thereby reducing the tendency of such compositions to become hazy.

Polyunsaturated fatty acid production in heterologous organisms using pufa polyketide synthase systems

Disclosed are novel acyl-CoA synthetases and novel acyltransferases, nucleic acid molecules encoding the same, recombinant nucleic acid molecules and recombinant host cells comprising such nucleic acid molecules, genetically modified organisms (microorganisms and plants) comprising the same, and methods of making and using the same. Also disclosed are genetically modified organisms (e.g., plants, microorganisms) that have been genetically modified to express a PKS-like system for the production of PUFAs (a PUFA PKS system or PUFA synthase), wherein the organisms have been modified to express an acyl-CoA synthetase, to express an acyl transferase, to delete or inactivate a fatty acid synthase (FAS) expressed by the organism, to reduce competition for malonyl CoA with the PUFA synthase or to increase the level of malonyl CoA in the organism, and in one aspect, to inhibit KASII or KASIII. Additional modifications, and methods to make and use such organisms, in addition to PUFAs and oils obtained from such organisms, are disclosed, alone with various products including such PUFAs and oils.

Process for the heterotrophic production of microbial products with high concentration of omega-3 highly unsaturated fatty acids

Pufa polyketide synthase systems and uses thereof

The invention generally relates to polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems isolated from or derived from non-bacterial organisms, to homologues thereof, to isolated nucleic acid molecules and recombinant nucleic acid molecules encoding biologically active domains of such a PUFA PKS system, to genetically modified organisms comprising PUFA PKS systems, to methods of making and using such systems for the production of bioactive molecules of interest, and to novel methods for identifying new bacterial and non-bacterial microorganisms having such a PUFA PKS system.

Methods for controlling highly unsaturated fatty acid content in various tissues

A method of treating a neurological disorder comprises administering to a person affected from such a disorder a microbial oil comprising DHA, a microbial oil comprising ARA or a combination of DHA and ARA oils in an amount sufficient to elevate the levels of circulating DHA and/or ARA in the person's blood to at least normal levels.

Schizochytrium and thraustochytrium strains for producing high concentrations of omega-3 highly unsaturated fatty acids

Disclosed is a process for growing the microflora Thraustochytrium, Schizochytrium , and mixtures thereof, which includes the growing of the microflora in fermentation medium containing non-chloride containing sodium salts, in particular sodium sulfate. In a preferred embodiment of the present invention, the process produces microflora having a cell aggregate size useful for the production of food products for use in aquaculture. Further disclosed is a food product which includes Thraustochytrium, Schizochytrium , and mixtures thereof, and a component selected from flaxseed, rapeseed, soybean and avocado meal. Such a food product includes a balance of long chain and short chain omega-3 highly unsaturated fatty acids.

Food products comprising long chain polyunsaturated fatty acids and methods for preparing the same

The present invention includes a food oil composition comprising a blend of a first oil comprising an LC PUFA and a second oil comprising substantially no LC PUFA. The first oil can preferably comprise an omega-3 LC PUFA, an omega-6 LC PUFA or mixtures thereof. The present invention also provides methods of food preparation, more particularly, methods for skillet-frying, deep-frying, methods for preparing edible lipid-containing food sauces, methods for preparing extruded food products, and methods for enhancing the LC PUFA content of a food product, particularly previously cooked food products, and food products prepared in accordance with such methods. Such compositions and methods are useful, for example, for increasing intake of LC PUFAs.

Reducing the risk of pathological effects of traumatic brain injury

The present disclosure provides methods and compositions for reducing the risk of pathological effects of traumatic brain injury.

Solventless extraction process

Enhanced production of lipids containing polyenoic fatty acid by very high density cultures of eukaryotic microbes in fermentors

The present invention provides a process for growing eukaryotic microorganisms which are capable of producing lipids, in particular lipids containing polyenoic fatty acids. The present invention also provides a process for producing eukaryotic microbial lipids.

Pufa polyketide synthase systems and uses thereof

Disclosed are the complete polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems from the bacterial microorganisms Shewanella japonica and Shewanella olleyana , and biologically active fragments and homologues thereof. More particularly, this invention relates to nucleic acids encoding such PUFA PKS systems, to proteins and domains thereof that comprise such PUFA PKS systems, to genetically modified organisms (plants and microorganisms) comprising such PUFA PKS systems, and to methods of making and using the PUFA PKS systems disclosed herein. This invention also relates to genetically modified plants and microorganisms and methods to efficiently produce lipids enriched in various polyunsaturated fatty acids (PUFAs) as well as other bioactive molecules by manipulation of a PUFA polyketide synthase (PKS) system.

Polyunsaturated fatty acids for treatment of dementia and pre-dementia-related conditions

本发明披露了用于治疗或预防痴呆和前痴呆相关病症和/或这类病症的症状或特征的组合物和方法。