Methods for Healthy Aging

Genome-wide association studies have been used to elucidate genes and/or pathways related to diseases; however, this methodology has yet to be used to understand the phenotype of healthy aging and/or healthspan in humans.

Methods of treating a neuroendocrine tumor

Disclosed herein, are methods of treating a neuroendocrine tumor (NET) in an individual in need thereof, comprising administering to the individual a therapeutically effective amount of an agent that inhibits a tropomyosin receptor kinase (Trk) protein, wherein the NET is associated with a Trk protein that has undergone a genetic translocation or is an NTRK gene fusion protein. Also disclosed herein are methods of treating a neuroendocrine tumor (NET) in an individual in need thereof, comprising: (a) obtaining a sample of NET genetic material from the individual; (b) determining whether the NET tumor comprises a NTRK translocation or gene fusion; and (c) administering to the individual a therapeutically effective amount of an agent that inhibits a tropomyosin receptor kinase (Trk) protein.

Apparatus and methods for balancing a joint

A joint replacement balancing system which provides real-time feedback to a surgeon during a joint replacement surgery to assist the surgeon to balance a joint replacement. The joint replacement balancing system includes a non-transitory processor-readable medium storing code representing instructions to cause a processor to receive a signal from a joint balancing apparatus, determine if the joint replacement is out of balance, determine a corrective course of action to bring the joint into balance and generate and display to the surgeon during the joint replacement surgery a recommended corrective course of action to complete the joint replacement surgery.

Methods of transplanting chondrocytes

Provided are tri-component matrices having collagen, hyaluronan, and chondroitin sulfate. Also provided are processes for producing a tri-component matrix. Additionally, provided are processes for providing cells capable of producing cartilage to a bone or cartilage defects.

Methods of transplanting chondrocytes

Provided are tri-component matrices having collagen, hyaluronan, and chondroitin sulfate. Also provided are processes for producing a tri-component matrix. Additionally, provided are processes for providing cells capable of producing cartilage to a bone or cartilage defects.

Predictive analysis for myocardial infarction

Compositions, systems and methods for the diagnosing the risk of acute myocardial infarction are provided. The methods described herein relate to the use of biomarkers, such as gene expression profiles, and analytical tools for providing information to a health care provider or the patient, that is relevant to the cardiovascular health of the patient.

METHODS FOR HEALTHY AGING

Genome-wide association studies have been used to elucidate genes and/or pathways related to diseases; however, this methodology has yet to be used to understand the phenotype of healthy aging and/or healthspan in humans.

Electrospinning of cartilage and meniscus matrix polymers

Disclosed herein are methods of producing a cartilaginous implant by producing a polymer scaffold composition by electrospinning a polymer solution onto a collector in order to obtain polymer fibers; crosslinking the polymer fibers; and adding a plurality of cells to the polymer scaffold composition, wherein the plurality of cells comprises cartilaginous cells to form a cartilaginous implant.

Methods of differentiating stem cells into chondrocytes

Disclosed herein are methods of producing chondrocytes from pluripotent stem cells. The invention further provides methods of regenerating cartilaginous tissue.

Tissue array printing

Methods of printing a bio-ink on a substrate are provided comprising at least one bio ink layer, said method comprising: i) positioning a printhead comprising a two-dimensional array of print nozzles within proximity of or in contact with a substrate; and ii) ejecting a bio-ink through the print nozzles onto the substrate, forming a bio ink layer, wherein the bio-ink construct comprises at least one bio-ink layer. The methods further encompass methods of printing a live tissue and methods of treating tissue defects.

Systems and methods to repair tissue defects

Methods of bioprinting a bio-ink construct on an internal tissue defect or a chondral defect during a minimally invasive surgery on an individual in need thereof are provided, comprising: visualizing the defect; positioning a bioprinter comprising a printhead within proximity of or in contact with the defect; and ejecting a bio-ink from the printhead onto the defect to form a bio-ink layer, thereby generating a bio-ink construct. Further provided are systems for bioprinting a bio-ink construct on an internal tissue defect during a minimally invasive surgery on an individual in need thereof, comprising a control system, an endoscope, and a bioprinter comprising a printhead.

In situ tissue engineering using magnetically guided three dimensional cell patterning

Methods are provided for the three dimensional manipulation of cells, and for the formation of an organized engineered cell tissue. Also provided are the organized engineered cell tissues produced by the methods. In one method, a plurality of magnetically labeled cells are mixed with a cross-linkable hydrogel to form a cell-hydrogel mixture, the at least a portion of the plurality of magnetically labeled cells are manipulated with a magnetic field to arrange the magnetically labeled cells into a specific cellular arrangement, and the hydrogel is crosslinked to form the organized engineered cell tissue. The approach presented herein offers a means to circumvent the deficiencies in the field of regenerative medicine, and allows for the production of organized tissues in situ with specific cellular organizations that mimic the native tissue.

Methods of reprogramming chondrocytes

Disclosed herein are methods of producing induced pluripotent stem cells from chondrocytes, and further, methods of producing chondrocytes from said induced pluripotent stem cells. The invention further provides methods of regenerating cartilaginous tissue.

Method of making a population of chondrocytes from reprogrammed chondrocytes

Disclosed herein are methods of producing induced pluripotent stem cells from chondrocytes, and further, methods of producing chondrocytes from said induced pluripotent stem cells. The invention further provides methods of regenerating cartilaginous tissue.

Methods of differentiating stem cells into chondrocytes

Disclosed herein are methods of producing chondrocytes from pluripotent stem cells. The invention further provides methods of regenerating cartilaginous tissue.

ELECTROSPINNING OF CARTILAGE AND MENISCUS MATRIX POLYMERS

Disclosed herein are methods of producing a cartilaginous implant by producing a polymer scaffold composition by electrospinning a polymer solution onto a collector in order to obtain polymer fibers; crosslinking the polymer fibers; and adding a plurality of cells to the polymer scaffold composition, wherein the plurality of cells comprises cartilaginous cells to form a cartilaginous implant.

Predictive analysis for myocardial infarction

Compositions, systems and methods for the diagnosing the risk of acute myocardial infarction are provided. The methods described herein relate to the use of biomarkers, such as gene expression profiles, and analytical tools for providing information to a health care provider or the patient, that is relevant to the cardiovascular health of the patient.

APPARATUS AND METHODS FOR BALANCING A JOINT

An apparatus includes a first portion configured to be coupled to a first bony structure and a second portion configured to be coupled between the first portion and a second bony structure. The second bony structure is disposed opposite the first bony structure. The apparatus further includes a transducer coupled between the first portion and the second portion. 1. An apparatus , comprising:a first portion configured to be coupled to a first bony structure;a second portion configured to be coupled between the first portion and a second bony structure, the second bony structure being disposed opposite the first bony structure; andat least one of a transducer and an actuator coupled between the first portion and the second portion.2. The apparatus of claim 1 , wherein the transducer is one transducer from a plurality of transducers claim 1 , each transducer from the plurality of transducers configured to output data associated with the apparatus.3. The apparatus of claim 2 , wherein the data includes at least one of force data claim 2 , position data claim 2 , displacement data or rotation data.4. The apparatus of claim 2 , wherein the transducer is configured to output the data during a surgical procedure.5. The apparatus of claim 1 , further comprising a mount coupled to the first portion claim 1 , the mount configured to be coupled to the first bony structure.6. The apparatus of claim 2 , wherein the mount is removably coupled to the first portion.7. The apparatus of claim 1 , further including an intermediate portion claim 1 , disposed between the first portion and the second portion claim 1 , configured to restrict the movement of the first portion relative to the second portion.8. The apparatus of claim 6 , wherein the intermediate portion is one of a post claim 6 , a bearing claim 6 , or a gasket.9. The apparatus of claim 1 , further including a flexible member claim 1 , disposed between the first portion and the second portion claim 1 , configured to limit a ...

METHODS OF TRANSPLANTING CHONDROCYTES

Provided are tri-component matrices having collagen, hyaluronan, and chondroitin sulfate. Also provided are processes for producing a tri-component matrix. Additionally, provided are processes for providing cells capable of producing cartilage to a bone or cartilage defects. 1. A tri-component matrix comprising:a) isolated and purified non-denatured type II collagen;b) hyaluronan; andc) chondroitin sulfate.2. (canceled)3. The tri-component matrix of claim 1 , further comprising a basal media.4. (canceled)5. The tri-component matrix of claim 1 , wherein the type II collagen is bovine-derived type II collagen.6. The tri-component matrix of claim 1 , wherein the concentration of type II collagen is from about 0.5 mg/ml to about 5 mg/ml.7. The tri-component matrix of claim 1 , wherein the concentration of type II collagen is about 3 mg/ml.8. The tri-component matrix of claim 1 , wherein the concentration of hyaluronan is from about 0.25 mg/ml to about 3 mg/ml.9. The tri-component matrix of claim 1 , wherein the concentration of hyaluronan is about 1 mg/ml.10. The tri-component matrix of claim 1 , wherein the concentration of chondroitin sulfate is from about 0.25 mg/ml to about 3 mg/ml.11. The tri-component matrix of claim 1 , wherein the concentration of chondroitin sulfate is about 1 mg/ml.12. The tri-component matrix of claim 1 , further comprising chondrocytes claim 1 , chondroprogenitor cells claim 1 , mesenchymal stem cells claim 1 , induced pluripotent stem cells (iPS cells) claim 1 , iPS cells derived from chondrocytes claim 1 , H9-derived chondroprogenitor cells claim 1 , Sox-9 transduced chondrocytes claim 1 , osteoblasts claim 1 , osteoprogenitors claim 1 , or any combinations thereof.13. The tri-component matrix of claim 1 , further comprising cells selected from chondrocytes claim 1 , chondroprogenitor cells claim 1 , and combination thereof.14. (canceled)15. The tri-component matrix of claim 1 , further comprising iPS cells.1627-. (canceled)28. A method ...

Systems and methods to repair tissue defects

Methods of bioprinting a bio-ink construct on an internal tissue defect or a chondral defect during a minimally invasive surgery on an individual in need thereof are provided, comprising: visualizing the defect; positioning a bioprinter comprising a printhead within proximity of or in contact with the defect; and ejecting a bio-ink from the printhead onto the defect to form a bio-ink layer, thereby generating a bio-ink construct. Further provided are systems for bioprinting a bio-ink construct on an internal tissue defect during a minimally invasive surgery on an individual in need thereof, comprising a control system, an endoscope, and a bioprinter comprising a printhead.

Apparatus and methods for balancing a joint

A joint replacement balancing system which provides real-time feedback to a surgeon during a joint replacement surgery to assist the surgeon to balance a joint replacement. The joint replacement balancing system includes a non-transitory processor-readable medium storing code representing instructions to cause a processor to receive a signal from a joint balancing apparatus, determine if the joint replacement is out of balance, determine a corrective course of action to bring the joint into balance and generate and display to the surgeon during the joint replacement surgery a recommended corrective course of action to complete the joint replacement surgery.

Method of analyzing cardiovascular disorders and uses thereof

Compositions, systems, and methods comprising circulating endothelial cells (CECs) are provided. The compositions described herein utilize isolated CECs, including compositions in a form that allows analysis of the CECs. The systems described herein utilize isolated CECs and an analytical tool or an output from an analytical tool. The methods described herein are related to the use of isolated CECs and analytical tools for providing information, to a health care provider or the CEC donor, that is relevant to the cardiovascular health of the CEC donor. Thus, the compositions, systems and methods described herein involve both a transformation (e.g., non-isolated CECs to isolated CECs, or isolated CECs to analyzed CECs) and a machine (e.g., isolation tools and analytical tools).

Method of analyzing cardiovascular disorders and uses thereof

Compositions, systems, and methods comprising circulating endothelial cells (CECs) are provided. The compositions described herein utilize isolated CECs, including compositions in a form that allows analysis of the CECs. The systems described herein utilize isolated CECs and an analytical tool or an output from an analytical tool. The methods described herein are related to the use of isolated CECs and analytical tools for providing information, to a health care provider or the CEC donor, that is relevant to the cardiovascular health of the CEC donor. Thus, the compositions, systems and methods described herein involve both a transformation (e.g., non-isolated CECs to isolated CECs, or isolated CECs to analyzed CECs) and a machine (e.g., isolation tools and analytical tools).

Methods of differentiating stem cells into chondrocytes

Predictive analysis for myocardial infarction

Compositions, systems and methods for the diagnosing the risk of acute myocardial infarction are provided. The methods described herein relate to the use of biomarkers, such as gene expression profiles, and analytical tools for providing information to a health care provider or the patient, that is relevant to the cardiovascular health of the patient.

Tissue array printing

Disclosed herein are methods of printing a bio-ink on a substrate. The methods further encompass methods of printing a live tissue and methods of treating tissue defects.

Systems and methods to repair tissue defects

Methods of bioprinting a bio-ink construct on an internal tissue defect or a chondral defect during a minimally invasive surgery on an individual in need thereof are provided, comprising: visualizing the defect; positioning a bioprinter comprising a printhead within proximity of or in contact with the defect; and ejecting a bio-ink from the printhead onto the defect to form a bio-ink layer, thereby generating a bio-ink construct. Further provided are systems for bioprinting a bio-ink construct on an internal tissue defect during a minimally invasive surgery on an individual in need thereof, comprising a control system, an endoscope, and a bioprinter comprising a printhead.

組織欠損を修復するためのシステムおよび方法

【課題】必要としている個体における低侵襲手術中に内部組織欠損または軟骨の欠損上にバイオインク構築物をバイオプリントする方法を提供する。【解決手段】方法は、欠損を視覚化する工程；欠損の近位またはそれに接する位置にプリントヘッドを含むバイオプリンターを配置する工程；およびバイオインク層を形成するために、欠損上にプリントヘッドからバイオインクを排出し、それによってバイオインク構築物を生成する工程、を含む。必要としている個体における低侵襲手術中に内部組織欠損上にバイオインク構築物をバイオプリントするためのシステムであり、システムは、制御システム、内視鏡、およびプリントヘッドを含むバイオプリンターを含む。【選択図】図６

Systems and methods to repair tissue defects

Methods of bioprinting a bio-ink construct on an internal tissue defect or a chondral defect during a minimally invasive surgery on an individual in need thereof are provided, comprising: visualizing the defect; positioning a bioprinter comprising a printhead within proximity of or in contact with the defect; and ejecting a bio-ink from the printhead onto the defect to form a bio-ink layer, thereby generating a bio-ink construct. Further provided are systems for bioprinting a bio-ink construct on an internal tissue defect during a minimally invasive surgery on an individual in need thereof, comprising a control system, an endoscope, and a bioprinter comprising a printhead.

Electrospinning of cartilage and meniscus matrix polymers

Electrospinning of cartilage and meniscus matrix polymers

Disclosed herein are methods of producing a cartilaginous implant by producing a polymer scaffold composition by electrospinning a polymer solution onto a collector in order to obtain polymer fibers; crosslinking the polymer fibers; and adding a plurality of cells to the polymer scaffold composition, wherein the plurality of cells comprises cartilaginous cells to form a cartilaginous implant.

Electrospinning of cartilage and meniscus matrix polymers

Electrospinning of cartilage and meniscus matrix polymers

Electrospinning of cartilage and meniscus matrix polymers

Disclosed herein are methods of producing a cartilaginous implant by producing a polymer scaffold composition by electrospinning a polymer solution onto a collector in order to obtain polymer fibers; crosslinking the polymer fibers; and adding a plurality of cells to the polymer scaffold composition, wherein the plurality of cells comprises cartilaginous cells to form a cartilaginous implant.

Cell injection printing with a coaxial needle

Provided are an apparatus and method for injection of a fluid into a substrate. In some embodiments, an apparatus or method described herein delivers cells to a tissue scaffold, graft, or site of tissue injury to facilitate the repair of a tissue defect.

Alk5 inhibitors for efficient derivation of mesenchymal stem cells from embryonic stem cells

Provided herein are methods for deriving mesenchymal stem cells from embryonic stem cells. In some cases, the methods provided herein involve the use of ALK5 inhibitors.

Dialdehyde starch crosslinked scaffold compositions and methods

Provided herein are compositions comprising collagen, dialdehyde starch, and at least one population of cells. Also, provided herein are methods of bioprinting and methods of producing cell-laden, three-dimensional scaffolds, comprising the compositions described herein.

Cell injection printing with a coaxial needle

Detection and isolation of myeloid-derived suppressor cell subpopulations

Myeloid derived suppressor cells (MDSCs) are a heterogeneous group of immature myeloid cells with the ability to mediate immunosuppression in cancer. Disclosed herein are methods of identifying MDSCs, methods of isolating MDSCs, and methods of treating patients.

Compositions and methods for disrupting a macrophage network

Described herein are methods and compositions for disrupting a macrophage network. Described herein are targeting agents capable of targeting one or more cells of a macrophage network. Methods for treating various diseases, such as cancer and granulomatous diseases are provided.

Dialdehyde starch crosslinked scaffold compositions and methods

Provided herein are compositions comprising collagen, dialdehyde starch, and at least one population of cells. Also, provided herein are methods of bioprinting and methods of producing cell-laden, three-dimensional scaffolds, comprising the compositions described herein.

治疗神经内分泌肿瘤的方法

本文公开了治疗有需要的个体的神经内分泌肿瘤(NET)的方法，包括向个体施用治疗有效量的抑制原肌球蛋白受体激酶(Trk)蛋白的药剂，其中NET与经历遗传易位的Trk蛋白或为NTRK基因融合蛋白的Trk蛋白相关。本文还公开了治疗有需要的个体的神经内分泌肿瘤(NET)的方法，包括：从个体获得NET遗传物质的样品；确定NET肿瘤是否包含NTRK易位或基因融合；以及向个体施用治疗有效量的抑制原肌球蛋白受体激酶(Trk)蛋白的药剂。

Methods of treating a neuroendocrine tumor

Disclosed herein, are methods of treating a neuroendocrine tumor (NET) in an individual in need thereof, comprising administering to the individual a therapeutically effective amount of an agent that inhibits a tropomyosin receptor kinase (Trk) protein, wherein the NET is associated with a Trk protein that has undergone a genetic translocation or is an NTRK gene fusion protein. Also disclosed herein are methods of treating a neuroendocrine tumor (NET) in an individual in need thereof, comprising: obtaining a sample of NET genetic material from the individual; determining whether the NET tumor comprises a NTRK translocation or gene fusion; and administering to the individual a therapeutically effective amount of an agent that inhibits a tropomyosin receptor kinase (Trk) protein.

Methods of treating a neuroendocrine tumor

Disclosed herein, are methods of treating a neuroendocrine tumor (NET) in an individual in need thereof, comprising administering to the individual a therapeutically effective amount of an agent that inhibits a tropomyosin receptor kinase (Trk) protein, wherein the NET is associated with a Trk protein that has undergone a genetic translocation or is an NTRK gene fusion protein. Also disclosed herein are methods of treating a neuroendocrine tumor (NET) in an individual in need thereof, comprising: obtaining a sample of NET genetic material from the individual; determining whether the NET tumor comprises a NTRK translocation or gene fusion; and administering to the individual a therapeutically effective amount of an agent that inhibits a tropomyosin receptor kinase (Trk) protein.

Dialdehyde starch crosslinked scaffold compositions and methods

治疗神经内分泌肿瘤的方法

本文公开了治疗有需要的个体的神经内分泌肿瘤(NET)的方法，包括向个体施用治疗有效量的抑制原肌球蛋白受体激酶(Trk)蛋白的药剂，其中NET与经历遗传易位的Trk蛋白或为NTRK基因融合蛋白的Trk蛋白相关。本文还公开了治疗有需要的个体的神经内分泌肿瘤(NET)的方法，包括：从个体获得NET遗传物质的样品；确定NET肿瘤是否包含NTRK易位或基因融合；以及向个体施用治疗有效量的抑制原肌球蛋白受体激酶(Trk)蛋白的药剂。