VORRICHTUNGEN UND VERFAHREN ZUM ENTFERNEN UNERWÜNSCHTER BESTANDTEILE AUS BLUTZELLEN

A bioprocessing system

Testing Filter Media

A fibrous filter medium is subjected to grafting of hydroxyethyl, hydroxyethyl methocrylate and carboxylic acid groups thereto, in order to present anionic groups in the fibre surfaces and thereby enhance its ability to remove leucocytes from blood plasma without depleting the platelets. The efficiency of the medium to do this is tested by passing an aqueous solution of cationic dye therethrough. The dye is initially adsorbed from the solution, but eventually the emerging liquid turns from colourless to coloured as the medium saturates. The quantity of solution passed before this happens gives a measure of the efficiency.

THE CONTROL OF GRAFTING PROCESSES IN THE PRODUCTION OF FIBROUS LEUKOCYTE DEPLETION FILTERS

Removing Impurities from Red Blood Cells

Red blood cells being salvaged are centrifuged into a liquid layer, mixed with a wash solution, and thereafter separated therefrom.

Automated system and method for processing biological fluid

PROCEDURE AND DEVICE FOR THE BLOOD TREATMENT WITH SENSORS FOR THE RECOGNITION OF CONTAMINATION

SYSTEM UND VERFAHREN ZUM BEARBEITEN VON BIOLOGISCHEN FLÜSSIGKEITEN

SEVERAL TREATMENT CHAMBERS COMPREHENSIVE SENTENCE AND VERWENDUNGSVERFAHEN

MEHRFACHBEUTELSYSTEM ZUR STERILEN BEHANDLUNG VON MENSCHLICHEN BLUTKONSERVEN

BLOOD CRACKING SYSTEM.

BLOOD CRACKING SYSTEM AND - PROCEDURES.

PROCEDURE AND DEVICE FOR A PACKAGING BIOLOGICAL OF ACTIVE SUBSTANCES IN CELLS

PROCEDURE AND DEVICE FOR THE TREATMENT OF HUMAN BLOOD FOR THE TRANSFUSION

Passive separation of whole blood

Described are systems, methods, and kits for compression sedimentation and whole blood separation. For example, a compression sedimentation system may include a compression stage configured to accept a flexible reservoir configured to contain a liquid mixture. The compression stage may include a base substrate and a compression substrate configured to apply a force to the flexible reservoir effective to create a pressure in the liquid mixture. An apparatus for whole blood separation may include a sedimentation system that separates whole blood into a supernatant including platelet rich plasma and a subnatant including red blood cells. At least one platelet-concentrating device may be included to receive the supernatant including the PRP and to separate a platelet concentrate and a platelet poor plasma from the supernatant.

Device and method for the analysis of rolling blood leukocytes

Confined air tube and methods for handling air in closed blood processing systems

System for treating transition zone material

BLOOD COMPONENT SEPARATION SYSTEM AND SEPARATION MATERIAL

The present invention relates to a separation system and a separation material for easily and rapidly separating a red blood cell-rich fraction, a white blood cell-rich fraction, and/or a platelet-rich fraction from a biological fluid containing these blood cell components without the necessity of performing centrifugation. The separation of a red blood cell-rich fraction, a white blood cell-rich fraction, and/or a platelet-rich fraction from a biological fluid can be accomplished by capturing white blood cells and platelets on a blood cell separation material by contacting the biological fluid with the blood cell separation material, thereby providing a red blood cell-rich fraction; and recovering a white blood cell-rich fraction by separating white blood cells captured from the resulting blood cell separation material using a separation solution.

APPARATUS FOR SEPARATION OF BLOOD COMPONENTS

An apparatus for the separation of blood components, specifically for the removal of white blood cells and blood platelets from blood comprises a tubular housing provided at one end thereof with a blood inlet and at the other end thereof with a blood outlet and an aggregate layer of fibers capable of sequestering white blood cells and blood platelets packed in the housing. The aggregate layer of fibers comprises a blood inlet side layer of a bulk density of not less than 0.16 g/cm3 and less than 0.21 g/cm3 and a blood outlet side layer of a bulk density of not less than 0.21 g/cm3 and not more than 0.23 g/cm3 and is packed in a ratio in the range of 0.04 to 0.09 g per ml of the flow volume of blood under treatment.

MULTIPLE PROCESSING CHAMBER SET AND USE THEREOF

A multiple sample processing apparatus for a continuous flow centrifuge, including a plurality of axially aligned processing chambers and expressor chambers, each chamber comprising an axial opening, in a fixed arrangement, and a plurality of central hubs disposed in the axial openings, the central hubs constructed and arranged to define passages for fluid communication between the chambers and a fluid supply.

AUTOMATED COLLECTION SYSTEMS AND METHODS FOR OBTAINING RED BLOOD CELLS, PLATELETS, AND PLASMA FROM WHOLE BLOOD

Blood processing systems and methods separate blood drawn from a donor into red blood cells and platelets. The systems and methods operate in a first mode to collect platelets while returning red blood cells to the donor. The systems and methods operate in a second mode to concurrently collect both platelets and red blood cells without returning platelets or red blood cells to the donor.

BLOOD COLLECTION SYSTEMS AND METHODS THAT DERIVE ESTIMATED EFFECTS UPON THE DONOR'S BLOOD VOLUME AND HEMATOCRIT

Blood processing systems and methods convey blood drawn from a donor through a blood processing circuit to separate the blood into at least one targeted blood component for collection. The systems and methods derive an estimated effect of the procedure upon the donor. The estimated effect can be expressed in terms of a net blood fluid volume loss, or as a hematodcrit of the donor after completion of the desired blood collection procedure. The systems and methods present the estimated effect to an operator for viewing, reading, or offloading.

PURIFICATION OF RED BLOOD CELLS BY SEPARATION AND DIAFILTRATION

Red blood cells are purified by separating whole blood, such as by centrifugation, to form a red blood cell fraction and a liquid fraction. The whole blood can be defibrinated or treated to prevent coagulation prior to separation. Preferably, the whole blood is bovine blood. The red blood cell fraction is then diafiltered to purify the red blood cells. The purified red blood cells can then be lysed to form a lysate of purified red blood cells. The purified red blood cells and the lysate of purified red blood cells are suitable for use in producing hemoglobin blood substitute.

A BIOPROCESSING SYSTEM

Disclosed is a bioprocessing system comprising apparatus (200) including a centrifugal separation housing (210) having a temperature controllable compartment (215) for removably accepting a separation chamber (50), the apparatus further comprising at least one mixing station (250) for supporting one or more fluid storage vessels (10, 20, 30, 40), the station including a temperature controllable area (252) for increasing or decreasing the temperature of the contents of the or each supported vessel. The system further includes a disposable fluidic arrangement (100) including a centrifugal separation chamber (50) removably mountable within the compartment (215) and having one or more ports (52) allowing fluid ingress into, or egress out of the chamber, via the one or more ports in use, said ports being in fluid communication with one or more of said fluid storage vessels via fluid conduits (12,22,32,42) and via one or more valve arrangements ...

APPARATUS AND KIT FOR ENCAPSULATING AT LEAST ONE COMPOUND FOR THERAPEUTIC AND/OR DIAGNOSTIC USE IN ERYTHROCYTES

A portable and highly automated apparatus and method for introducing at least one compound within erythrocytes; the apparatus (1) comprises a reusable part (56) provided with mechanical elements such as pumps (31, 40, 49) and valves (10, 26, 29, 30, 33, 46, 50) and electronic units such as a control unit (15); the apparatus (1) also comprises a disposable part (55), which is adapted to come into contact with the sample containing the erythrocytes and is provided with a system (2) of tubes made of deformable material, a plurality of reservoirs (6, 13, 21, 27, 28, 39, 48) and one or more filters (37); the apparatus (2) allows a further concentration of the erythrocytes after they have been treated; the apparatus (1) allows to introduce the compound in the erythrocytes in a virtually totally automated manner.

METHOD OF BLOOD POOLING AND STORAGE

The disclosure provides methods of making a cell-containing product having a uniform amount of cells therein. The method comprises pooling red blood cells from a plurality of blood units, and inactivating any pathogen contained therein. A storage solution added to the cellular component results in a cell-containing product that is essentially pathogen and white blood cell free and has an extended shelf life of about 42 to about 100 days. The cell-containing product is further divided into units which comprise a uniform dose of RBCs per unit.

DEVICE AND METHOD FOR THE ANALYSIS OF ROLLING BLOOD LEUKOCYTES AND IDENTIFYING INHIBITORS AND PROMOTERS

... 2110292 9221746 PCTABS00017 The present invention provides in vitro models of the in vivo) rolling and arrest of leukocytes along the endothelial cell wall, which are important steps in the migration of leukocytes out of the blood stream and into tissue, as part of the inflammatory response. The in vitro models of the invention are functional under physiologic flow conditions resulting in physiologic shear stresses. In a specific embodiment, for modelling leukocyte rolling, the apparatus of the invention comprises a solid phase surface with rolling mediator molecules present thereon. Such rolling mediators are, for example, selectins and selectin ligands which have binding partners expressed on leukocytes. In another specific embodiment, for modelling leukocyte rolling followed by adhesion/arrest, the apparatus of the invention comprises a solid phase surface with both rolling mediators and integrin binding partners present thereon. The apparatuses of the invention can be used for collecting ...

AUTOMATION OF PLASMA SEQUESTRATION

A plasma sequestration system having a volume control system is provided. When a calculated volume of whole blood has been processed, the system switches from a fill to an empty cycle. The termination point is calculated in advance, based on the desired composition of the collected plasma, thus improving process control and product uniformity. The system is automated or partially automated using an optical sensor or marker to monitor the volume of packed red blood cells. When the red cells reach the marker or sensor, a signal is sent to the control system which then calculates the required fill volume. Processing continues until the calculated fill volume is reached, at which time the system automatically stops the collection cycle. The system also provides for two speed operation of the centrifuge as a safeguard against foaming which may result in aerosol formation and blood trauma. Foaming is reduced by reducing centrifuge speed as the packed red blood cells approach the stationary deflector ...

WASHING CELLS

Red blood cells being salvaged are centrifuged into a liquid layer, mixed with a wash solution, and thereafter separated therefrom.

METHOD AND APPARATUS FOR ENCAPSULATION OF BIOLOGICALLY-ACTIVE SUBSTANCES IN CELLS

The present invention relates to a method and apparatus for the encapsulatio n of biologically-active substances in red blood cells, characterized by an optionally automated, continuous-flow self-contained electroporation system (10), as shown in the figure which allows withdrawal of blood from a patient (11), separation of red blood cells (44), encapsulation of a biologically-active substance in the cells (72), and optional recombination of blood plasma and the resulting modified red blood cells (96), thereby producing blood with modified biological characteristics. The present invention is particularly suited for use to encapsulate allosteric effectors of hemoglobin, thereby reducing the affinity of erythrocytes for oxygen and improving the release of oxygen from erythrocytes in tissues.

METHODS FOR PROCESSING RED BLOOD CELL PRODUCTS FOR LONG TERMSTORAGE FREE OF MICROORGANISMS

A blood processing method provides a red blood cell product that is free of microorganisms like Yersinia enterocoli- tica during storage periods over 24 hours. The red blood cell product is collected in a first container (16), where it is refrige- rating to cool the blood product to a temperature of about 3 to degrees C. The refrigerated product is transferred from the first container (16) into a storage container (34) through a pre- scribed filter medium (44) that comprises a mass of synthetic fibers having an average fiber diameter of about 10 microns or less and a bulk density of about 0.7 gram per cubic centimeter or less. The filtered product is retained in the storage container (34) at a temperature of about 3 to 5 degrees C for a storage pe- riod over 24 hours after filtration. Using the filter medium (44), microorganisms like Yersinia enterocolitica present in the red blood cell product at the time of collection are depleted. The filtered blood product remains free of clinically ...

Automated device for processing a biological fluid and its use.

PROCEDE POUR SEPARER LES COMPOSANTS DES CELLULES DU SANG

Procédé de séparation des composants des cellules du sang. Le procédé consiste à faire passer une suspension des cellules du sang contenant les cellules rouges et au moins un groupe des leucocytes et plaquettes sanguines à travers une colonne remplie de coton égyptien dégraissé et blanchi de façon à permettre le composant des cellules du sang autres que les cellules rouges d'être adsorbé sur le coton égyptien et à isoler la partie cellules rouges seule.

METHOD OF BLOOD POOLING AND STORAGE

The disclosure provides methods of making a cell-containing product having a uniform amount of cells therein. The method comprises pooling red blood cells from a plurality of blood units, and inactivating any pathogen contained therein. A storage solution added to the cellular component results in a cell-containing product that is essentially pathogen and white blood cell free and has an extended shelf life of about 42 to about 100 days. The cell-containing product is further divided into units which comprise a uniform dose of RBCs per unit.

MEMBRANE SEPARATION DEVICES, SYSTEMS AND METHODS EMPLOYING SAME, AND DATA MANAGEMENT SYSTEMS AND METHODS

A membrane separation device is disclosed along with systems and methods employing the device in blood processing procedures. In one embodiment, a spinning membrane separator is provided in which at least two zones or regions are created in the gap between the spinning membrane and the shell, such that mixing of the fluid between the two regions is inhibited by a radial rib or ridge associated with the spinning membrane that decreases the gap between the spinning membrane and the shell to define two fluid regions, the ridge isolating the fluid in the two regions to minimize mixing between the two. Automated systems and methods are disclosed for separating a unit of previously-collected whole blood into selected blood components, such as concentrated red cells and plasma, for collecting red cells and plasma directly from a donor in a single pass, and for cell washing. Data management systems and methods and priming methods are also disclosed.

Red blood cell storage system

Closed blood bag system for long term storage of red blood cells (RBCs) without contamination due to storage. System comprises the combination of a white blood cell (WBC) filter in sterile communication with a plastic bag made from a film substantially free of blood extractable plasticizers. In use, an RBC preservative solution (preferably from within the closed system) is added to a mixture of RBCs and WBCs and the mixture is then passed through the filter to remove at least 98% of the WBCs. The filtered RBCs and preservative solution pass into the attached bag and can then be stored in the attached bag for up to 42 days.

Method for processing blood for human transfusion

The invention discloses a method for treating blood comprising (a) centrifuging blood and (b) passing a supernatant layer of the centrifuged blood through a porous medium until red blood cells block the porous medium.

Method of blood pooling and storage

The disclosure provides methods of making a cell-containing product having a uniform amount of cells therein. The method comprises pooling red blood cells from a plurality of blood units, and inactivating any pathogen contained therein. A storage solution added to the cellular component results in a cell-containing product that is essentially pathogen and white blood cell free and has an extended shelf life of about 42 to about 100 days. The cell-containing product is further divided into units which comprise a uniform dose of RBCs per unit.

Whole blood separation system

A system and method are provided for controlling fouling and complement protein activation during separation of plasma from whole blood using a spinning membrane separator. The separator includes a pair of relatively rotating surfaces spaced apart to define a gap therebetween, with at least one of the surfaces comprising a membrane that allows plasma to pass therethrough but substantially prevents the passage of red cells. In accordance with the method, the membrane material and membrane fabrication technique are selected so as that the resulting membrane both resists fouling and complement protein activation. In a specific embodiment, the membrane is has a smooth surface and substantially linear pores. The pores have a nominal diameter of less than 2 microns (so as to exclude platelets) and preferably a diameter of from 0.6 microns to 0.8 microns, as may be obtained by use of track-etching. In addition, the membrane material preferably is polycarbonate, as it has been determined that polycarbonate ...

ALL-IN-ONE MEANS OF SEPARATING BLOOD COMPONENTS

SYSTEMS AND METHODS FOR REMOVING UNDESIRED MATTER FROM BLOOD CELLS

Systems and methods of collecting blood cells use a first container (16) connected to at least three transfer containers (18, 26, 34). A filtration system (10) is provided with one transfer container (34), which ultimately serves as the storage container for red blood cells free of white blood cells. The two other transfer containers (18, 26) are connected to each other, one (18) of which is empty and one (26) or which contains an additive solution (5) for storage of red blood cells. The empty transfer container (18) is used for storage of platelet concentrate, while the container (26) that originally contained the additive solution is ultimately used to store the platelet-poor plasma component. A portion of the additive solution (5) may be separately passed through the filtration system (10) after filtration of the red cells to flush additional red blood cells from the filtration device (40).

BLOOD COMPONENT SEPARATOR

FLUID MANAGEMENT SYSTEM AND METHODS

A hysteroscopic fluid management system includes a saline source with an electrolyte concentration, at least one pressure mechanism for circulating saline to and from a targeted site and through a filter having filter characteristics back to the source, and a controller. The controller provides a saline inflow in a first flow path to the site and a saline outflow in a second flow path from the site through the filter and back to the source at a controlled flow rate. A diagnostic or therapeutic procedure is performed at the site in the presence of the saline. The filter characteristics and the controlled flow rate are selected to (1) cause substantially no change in the electrolyte concentration in the saline, (2) to prevent hemolysis of greater than 5% of filtered red blood cells exposed to the saline, and/or (3) to minimize effect on prothrombin time of plasma exposed to the filter.

GERÄT UND VERFAHREN ZUR VERARBEITUNG VON BIOLOGISCHEN ZELLEN

METHODE UND VERWENDUNG EINES FILTERS ZUR ENTFERNUNG VON LEUCOCYTEN

INJIZIERBARE, STROMAFREIE HAEMOGLOBINLOESUNG UND VERFAHREN ZU IHRER HERSTELLUNG

Automated system and method for processing biological fluid

FLOW/RETURN OF BLOOD CELLS TO A PATIENT/DONOR AFTER CENTRIFUGAL SEPARATION

STROMA-FREE HAEMOGLOBIN

APPARATUS FOR BRINGING OF MEDICINES INTO RED BLOOD CORPUSCLES

VERFAHREN ZUR BEHANDLUNG EINES BIOLOGISCHEN FLUIDS

VERFAHREN ZUR BEHANDLUNG EINES BIOLOGISCHEN FLUIDS

SYSTEM AND PROCEDURE FOR WORKING ON BIOLOGICAL LIQUIDS

System and method for processing biological fluid

BLOOD FRACTIONATION SYSTEM AND METHOD

SYSTEMS AND METHODS FOR REMOVING UNDESIRED MATTER FROM BLOOD CELLS

Apparatus for extracting a blood component contained in a system of bags

The invention relates to an apparatus (9) for extracting at least one blood component contained in a primary bag (1) of a system of bags, said system of bags further including at least one bag (4) of solution containing an additive solution for said blood component, and at least one satellite bag (3) for collecting said blood compound with said additive solution added thereto, said bags (1, 3, 4) being connected to one another by tubes, said apparatus including: an extraction device (14) for extracting the blood compound by compressing the primary bag (1), a monitoring device (15) for enabling said extracted compound or said combination of the extracted compound and the additive solution to be transferred to the collecting satellite bag (3) via the tubes, an agitation device (16) for agitating the collecting satellite bag (3), and a drive system for controlling said extraction device (14), said monitoring device (15) and the agitation device (16), said system being designed so as to enable ...

BLOOD BAG SYSTEM WITH INTEGRAL FILTERING MEANS

BLOOD COMPONENT SEPARATION SYSTEM AND SEPARATION MATERIAL

Provided are a system and separation material that can quickly and easily separate a bodily fluid containing various blood cell components into an erythrocyte-rich fraction, a leukocyte-rich fraction, and a thrombocyte-rich fraction, without requiring centrifuging. The provided method includes: a step in which the bodily fluid is brought into contact with a blood cell separation material and leukocytes and thrombocytes are captured, yielding an erythrocyte-rich fraction; and a step in which a separation solution is used to collect a leukocyte fraction captured by the blood cell separation material.

METHOD AND SYSTEM FOR REMOVING OXYGEN AND CARBON DIOXIDE DURING RED CELL BLOOD PROCESSING USING AN INERT CARRIER GAS AND MANIFOLD ASSEMBLY

A portable assembly for processing red blood cells RBCs including a disposable blood collection set including a blood bag, an anaerobic storage bag and an oxygen and/or oxygen and carbon dioxide depletion device disposed between the blood collection bag and anaerobic storage bag. The portable assembly further provides for a gas circulation device in fluid communication with the oxygen or oxygen and carbon dioxide depletion device, The gas circulation device includes a pressure source that is able circulate flushing gas through the depletion device as RBCs pass from the blood collection bag, through the depletion device and into the anaerobic storage bag.

AUTOMATED SYSTEM AND METHOD FOR PROCESSING BIOLOGICAL FLUID

An automated system for processing biological fluid includes a pressure differential generator; a biological fluid processing assembly, and an automated control arrangement coupled to at least one of the pressure differential generator and the biological fluid processing assembly. The automated system may include at least one porous medium in a housing to form a filter assembly, such as a red cell barrier assembly, a leukocyte depletion filter assembly and a red cell barrier leukocyte depletion filter assembly.

SYSTEM FOR TREATING TRANSITION ZONE MATERIAL

Methods and devices for treating transition zone material are disclosed. Transition zone material in a plurality of source containers (20) passes through the conduits (40) of the pooling assembly (21) through junction (50) and then to receiving container (22). The transition zone material in the receiving container (22) is processed to form a supernatant layer which includes platelets and a sediment layer which includes red blood cells, and the supernatant layer is separated from the sediment layer by passing the supernatant layer through a porous medium such as a red cell barrier medium (70) into a satellite container (80). Gas may be separated by passing it into a gas collection and displacement loop (300) including first and second conduits (160, 170) and a liquid barrier medium (150).

RED BLOOD CELL STORAGE SYSTEM

Closed blood bag system for long term storage of red blood cells (RBCs) without contamination due to storage. System comprises the combination of a white blood cell (WBC) filter in sterile communication with a plastic bag made from a film substantially free of blood extractable plasticizers. In use, an RBC preservative solution (preferably from within the closed system) is added to a mixture of RBCs and WBCs and the mixture is then passed through the filter to remove at least 98% of the WBCs. The filtered RBCs and preservative solution pass into the attached bag and can then be stored in the attached bag for up to 42 days.

TREATMENT OF THE MATERIAL OF THE TRANSITION ZONE.

APPARATUS FOR EXTRACTING A BLOOD COMPONENT CONTAINED IN A POCKET SYSTEM

APPARATUS FOR EXTRACTING A BLOOD COMPONENT CONTAINED IN A POCKET SYSTEM

L'invention concerne un appareil (9) pour extraire au moins un composant sanguin contenu dans une poche primaire (1) d'un système à poches, ledit système à poches comprenant en outre au moins une poche (4) de solution contenant une solution additive pour ledit composant sanguin et au moins une poche satellite (3) de recueil destinée à recueillir ledit composant sanguin additionné de ladite solution additive, lesdites poches (1,3,4) étant associées entre elles par des tubulures, ledit appareil comprenant : - un dispositif d'extraction (14) destiné à extraire le composant sanguin par compression de la poche primaire (1), - un dispositif de contrôle (15) destiné à permettre le transfert dudit composant extrait ou dudit composant extrait et de la solution additive, dans la poche satellite (3) de recueil par l'intermédiaire des tubulures, - un dispositif d'agitation (16) destiné à agiter la poche satellite (3) de recueil, et - un système de pilotage pour commander ledit dispositif d'extraction (14), ledit dispositif de contrôle (15) et le dispositif d'agitation (16), ledit système étant conçu pour permettre le mélange homogène du composant sanguin extrait et de la solution additive.

Automated system for processing biological fluid, esp. blood

METHOD FOR PRECIPITATING RED BLOOD CELLS

A method for washing collected blood uses an inert anticoagulant during collection of the blood. The inert anticoagulant does not interfere with agglomeration of the red blood cells so that gravity sedimentation separation is facilitated. The preferred inert anticoagulant is CPD.

METHODS FOR PROCESSING RED BLOOD CELL PRODUCTS FOR LONG TERM STORAGE FREE OF MICROORGANISMS

A blood processing method provides a red blood cell product that is free of microorganisms like Yersinia enterocolitica during storage periods over 24 hours. The red blood cell product is collected in a first container (16), where it is refrigerating to cool the blood product to a temperature of about 3 to 5 degrees C. The refrigerated product is transferred from the first container (16) into a storage container (34) through a prescribed filter medium (44) that comprises a mass of synthetic fibers having an average fiber diameter of about 10 microns or less and a bulk density of about 0.7 gram per cubic centimeter or less. The filtered product is retained in the storage container (34) at a temperature of about 3 to 5 degrees C for a storage period over 24 hours after filtration. Using the filter medium (44), microorganisms like Yersinia enterocolitica present in the red blood cell product at the time of collection are depleted. The filtered blood product remains free of clinically significant ...

System and method for processing biological fluid

A system for collecting and processing donated blood comprises a first porous medium interposed between a blood collection bag and a satellite bag and a second porous medium interposed between the blood collection bag and another satellite bag. The porous media are leucocyte depletion media. The system may also include one or more of the following: a red cell barrier medium, a separation medium, a gas inlet, and a gas outlet. The system can be used to centrifuge whole blood into one or more components, and includes a means for protecting the system during centrifugation.

Apparatus and Method for Separating Discrete Volumes of A Composite Liquid

An apparatus for separating at least two discrete volumes of a composite liquid into at least a first component and a second component comprises a centrifuge, which comprising a rotor having a rotation axis, comprising at least two separation cells, each for containing a separation bag containing a volume of composite liquid; and a first balancing means for balancing the rotor when the respective weights of the at least two separation bags in the at least two separation cells are different.

Rotating seals for cell processing systems

The invention provides an improved rotating seal apparatus including a plurality of concentrically spaced annular rotating seals. The invention also provides a rotating seal apparatus including a pressurized annular chamber surrounding an annular rotating seal.

Liquid filtering device

BLOOD COMPONENT SEPARATOR

Separator for leucocytes and platelets from blood A separator for blood components comprises a cylindrical housing with ports for blood introduction and exit, packed with at least two layers of fibrous aggregate in series, the layer on the inlet side having a bulk density of 0.16-0.21 g/cm3 and that on the outlet side having a bulk density of 0.21-0.23 g/cm3. The inlet side layer may comprise two successive layers of fibrous aggregate having respectively bulk densities of 0.16-0.19 and 0.19-0.21 g/cm3. The wt. ratio of the layers is pref. 75:25-60:40. The separator is packed with 0.04-0.09 g fibrous aggregate per 1 ml of blood to be treated. The fibre is pref. short fibres and may be natural cotton, polyester, polyamide, polyacrylonitrile or cellulose acetate. Pref. is defatted, bleached Egyptian cotton. The removal of leucocytes and platelets from blood.

УСТРОЙСТВО И НАБОР ДЛЯ ИНКАПСУЛЯЦИИ В ЭРИТРОЦИТАХ ПО МЕНЬШЕЙ МЕРЕ ОДНОГО СОЕДИНЕНИЯ ДЛЯ ТЕРАПЕВТИЧЕСКОГО И ДИАГНОСТИЧЕСКОГО ПРИМЕНЕНИЯ

... 1. Устройство для введения по меньшей мере одного соединения в эритроциты; при этом устройство (1) содержит систему (2) соединительных каналов, которая включает в себя первый и второй каналы (9, 10); блок (3) введения для введения пробы, содержащей эритроциты, в устройство (1); разделительный блок (4) для разделения между собой разных компонентов пробы; объединительный блок (5), который содержит первую емкость (6), и в зоне которого эритроциты и соединение объединяют для получения обработанных эритроцитов; входное отверстие (7) для ввода соединения в первую емкость (6); питающий блок (8) для подачи первого раствора по первому каналу (9) и подачи второго раствора по второму каналу (10); концентрирующий блок (11) для концентрирования содержимого первой емкости (6); и сборный блок (12), который содержит вторую емкость (13) для сбора обработанных эритроцитов;причем система (2) каналов соединяет блок (3) введения, разделительный блок (4), объединительный блок (5), питающий блок (8), концентрирующий ...

Separationseinrichtung zur Trennung von Blut

Vorrichtung zum Filtrieren von Blutplättchen.

Vorrichtung und Verfahren zur Überwachung eines Gefäßzugangs für eine extrakorporale Blutbehandlung

Die Erfindung betrifft eine Vorrichtung und ein Verfahren zur Überwachung eines Zugangs zu einem Patienten für eine extrakorporale Blutbehandlungsvorrichtung mit einem extrakorporalen Blutkreislauf. Darüber hinaus betrifft die Erfindung eine extrakorporale Blutbehandlungsvorrichtung mit einer Vorrichtung zur Überwachung des Gefäßzugangs. Die erfindungsgemäße Vorrichtung und das erfindungsgemäße Verfahren beruhen auf der Überwachung einer charakteristischen Eigenschaft des Bluts, insbesondere der Konzentration von Hämoglobin in dem in der arteriellen Blutleitung des extrakorporalen Blutkreislaufs I einer extrakorporalen Blutbehandlungsvorrichtung A strömenden Blut. Bei einem nicht ordnungsgemltnisse im kommunizierenden intra- und extrakorporalen Blutzirkulationssystem. Diese Veränderungen der Strömungsverhältnisse sind als Änderung der Hämoglobinkonzentration nachweisbar. Eine Diskonnektion der venösen Punktionskanüle 8 für den Patientenzugang wird durch eine Verringerung der Hämoglobinkonzentration ...

VERFAHREN ZUR BEHANDLUNG VON PRODUKTEN ROTER BLUTKÖRPERCHEN ZUR LANGZEITLAGERUNG FREI VON MIKROORGANISMEN.

Positioning of filter assembly in centrifugal processing of biological fluids

A system for processing a biological fluid, especially blood, comprises at least one container which fits in a centrifuge bucket 120, at least one filter assembly 114 in fluid communication with the container and a bracket 122, adapted to receive the filter assembly, which is cooperatively arranged with the bucket. A method for processing a biological fluid, especially blood, using a processing apparatus (comprising a first and second container and a filter assembly interposed therebetween) comprises collecting a biological fluid in the first container, placing the processing apparatus in a centrifuge bucket and positioning the filter assembly away from the bottom of the bucket prior to centrifuging. Preferably the positioning is achieved using a bracket which rests on or partly within the centrifuge bucket. ...

System and method for processing biological fluids

Automated system and method for processing biological fluid

Flow/return of blood cells to a patient/donor after centrifugal separation

Returning red blood cells to a patient after a centrifuge separation procedure by connecting an inflow line of a separation channel to an outflow line to provide a closed loop and recirculating liquid through it to remove blood cells. Also disclosed is pulling more liquid out of a flexible separation channel than permitted into it in order to reduce the volume of liquid to be used in returning cells to the patient.

Surgical swab washing method and apparatus

AUTOMATIC SYSTEM FOR PLASMA SEPARATION

PROCEDURE AND DEVICE FOR THE TREATMENT OF BLOOD FOR THE HUMAN ONE TRANSFUSION

SEPARATE ION MECHANISM FOR THE SEPARATION FROM BLOOD INTO ITS COMPONENTS.

MULTIPLE BAG SYSTEM FOR THE STERILE TREATMENT OF HUMAN UNITS OF STORED BLOOD

AUTOMATED TREATMENT SYSTEM F�R BIOLOGICAL FLUIDS AND PROCEDURE HIEZU

MEHRFACHBEUTELSYSTEM ZUR STERILEN BEHANDLUNG VON MENSCHLICHEN BLUTKONSERVEN

PROCEDURE AND DEVICE FOR THE BLOOD TREATMENT WITH SENSORS FOR THE RECOGNITION OF CONTAMINATION

AUTOMATED TREATMENT OF BLOOD

Centrifuge system and automated processing of blood

Apparatus and kit for encapsulating at least one compound for therapeutic and/or diagnostic use in erythrocytes

A portable and highly automated apparatus and method for introducing at least one compound within erythrocytes; the apparatus (1) comprises a reusable part (56) provided with mechanical elements such as pumps (31, 40, 49) and valves (10, 26, 29, 30, 33, 46, 50) and electronic units such as a control unit (15); the apparatus (1) also comprises a disposable part (55), which is adapted to come into contact with the sample containing the erythrocytes and is provided with a system (2) of tubes made of deformable material, a plurality of reservoirs (6, 13, 21, 27, 28, 39, 48) and one or more filters (37); the apparatus (2) allows a further concentration of the erythrocytes after they have been treated; the apparatus (1) allows to introduce the compound in the erythrocytes in a virtually totally automated manner.

SEPARATION OF CELLS FROM A CELLULAR SUSPENSION

CENTRIFUGAL SEPARATION OF BLOOD

Automated system and method for processing biological fluid

INTEGRATED LEUKOCYTE, OXYGEN AND/OR CO2 DEPLETION, AND PLASMA SEPARATION FILTER DEVICE

A method for processing blood, comprising passing blood through a blood filter device (63). The filter device (63) comprises a housing comprising an outer wall, a first inlet (85), a first outlet (83) and a second outlet (83); a membrane (73) which is capable of separating plasma from blood. The membrane (73) forms at least one inner chamber (69) within the housing and blood enters the at least one inner chamber of the blood filter device through the first inlet (85). A leukocyte and oxygen depletion media (67) is disposed within the at least one inner chamber (69) and which is capable of depleting leukocytes and oxygen from said blood. The device (63) also has an outer chamber (77) disposed between the outer wall and the membrane (73). The method separates plasma from the blood by permeating through the membrane (73), wherein the plasma enters said outer chamber (77) and exits the housing via the first outlet (83) and depleting oxygen, leukocytes and plasma from the blood, whereby oxygen ...

Cell filter separation system

Separation of a wash liquid from red blood cells is disclosed using a filter separation and pressure differential system. The filter may include a membrane filter.

CENTRIFUGE

Centrifuges are useful to, among other things, remove red blood cells from whole blood and retain platelets and other factors in a reduced volume of plasma. Platelet rich plasma (PRP) and or platelet poor plasma (PPP) can be obtained rapidly and is ready for immediate injection into the host. Embodiments may include valves, operated manually or automatically, to open ports that discharge the excess red blood cells and the excess plasma into separate receivers while retaining the platelets and other factors in the centrifuge chamber. High speeds used allow simple and small embodiments to be used at the patient's side during surgical procedures. The embodiments can also be used for the separation of liquids or slurries in other fields such as, for example, the separation of pigments or lubricants. 127-. (canceled)30. A centrifuge for isolating and concentrating a fraction of a biologic liquid mixture comprising:a. a chamber having a longitudinal axis, a first end, a second end, and a sidewall extending between said first end and said second end, wherein at least a portion of said sidewall is transparent;b. a first port provided in said chamber at a first radial distance from said longitudinal axis, and in fluid communication with a first valve;c. a second port provided in said chamber at a second radial distance from said longitudinal axis, and in fluid communication with a second valve, said second radial distance being less than said first radial distance;d. a motor configured to rotate said chamber about said longitudinal axis and thereby produce a centrifugal field;wherein the sidewall is configured to direct toward the first port a biologic liquid mixture that is present in the chamber and experiencing a centrifugal field.31. The centrifuge of claim 30 , wherein the sidewall extends from the first end to the second end in a first direction that is away from the longitudinal axis.32. The centrifuge of claim 30 , wherein the sidewall extends from the first end to ...

PLASMAPHERESIS DEVICE

Apparatus for treating blood. A device () for separating plasma from blood comprises a blood flow path () including formations () for agitating blood flow along the blood flow path and a separation membrane () with a first surface in fluid communication with the blood flow path. The device may be comprised in an extracorporeal blood circuit and used in a method for separating plasma from blood. 1. A device for separating plasma from blood , the device comprising:a blood flow path;a separation membrane having a first surface in fluid communication with the blood flow path; andformations located in the blood flow path for agitating blood flow along the blood flow path.2. The device of claim 1 , wherein the separation membrane is substantially permeable to blood plasma but substantially impermeable to one or more other components of blood claim 1 , such as red blood cells and platelets.3. The device of claim 2 , wherein the separation membrane has an average pore size of between 0.5 μm and 5 μm claim 2 , between 1 μm and 4 μm claim 2 , between 1.5 μm and 3 μm claim 2 , or around 2 μm.4. The device of claim 1 , wherein the separation membrane has an air flow rate at 200 Pa of at least 10 l/m claim 1 , at least 15 l/mor at least 20 l/m.5. The device of claim 1 , wherein blood flow path is formed of a substantially transparent material or comprises a substantially transparent portion.6. The device of claim 1 , wherein the separation membrane forms at least 20% claim 1 , at least 30% or at least 40% of the surface area of the blood flow path.7. The device of claim 1 , wherein the separation membrane forms a relatively lowest portion of the surface of the blood flow path.8. The device of claim 1 , wherein the ratio between the length and the width of the blood flow path may be at least 10:1 claim 1 , at least 50:1 or at least 100:1.9. The device of claim 1 , wherein the blood flow path is convoluted.10. The device of claim 9 , wherein the blood flow path is made up of ...

DIALYZER CONTROL APPARATUS AND DRIVING METHOD THEREOF

A the dialyzer control apparatus includes a magnetic field generator which is disposed on an outer surface of a dialyzer which removes wastes from a blood flowing therein and generates a magnetic field; and a controller which controls the magnetic field generator to perform a magnetic field stimulation on the blood flowing into the dialyzer. The magnetic field stimulation may be a stimulation related to the improvement of the Rouleau formation for the red blood cells in the blood. 1. A dialyzer control apparatus , comprising:a magnetic field generator which is disposed on an outer surface of a dialyzer which removes wastes from a blood flowing therein to discharge and generates a magnetic field; anda controller which controls the magnetic field generator to perform a magnetic field stimulation on the blood flowing into the dialyzer,wherein the magnetic field stimulation is a stimulation related to improvement of Rouleau formation for red blood cells in the blood.2. The dialyzer control apparatus according to claim 1 , wherein the magnetic field stimulation is a stimulation related to a blood ionization acceleration by binding oxygen to iron ions included in hemoglobin of the red blood cells.3. The dialyzer control apparatus according to claim 1 , wherein the magnetic field stimulation is a stimulation related to acceleration of at least one of anti-inflammatory claim 1 , anti-cancer claim 1 , and antimicrobial effects due to generation of nitric oxide (NO) in the blood.4. The dialyzer control apparatus according to claim 1 , wherein the controller controls at least one of an intensity claim 1 , a frequency claim 1 , a time claim 1 , and a stimulation mode of the magnetic field as a type of the magnetic field generated from the magnetic field generator.5. The dialyzer control apparatus according to claim 4 , wherein the controller controls the frequency of the magnetic field to any one of 8 Hz to 30 Hz andthe magnetic field generator generates the magnetic field ...

Integrated Leukocyte, Oxygen and/or CO2 Depletion, and Plasma Separation Filter Device

A blood filter device comprising: a housing comprising an outer wall and a first inlet, a first outlet and a second outlet; a membrane which is capable of separating plasma from the blood, wherein the membrane forms an inner chamber; a leukocyte and oxygen and/or carbon dioxide depletion media disposed wherein the inner chamber, the leukocyte and oxygen and/or carbon dioxide depletion media is capable of depleting leukocytes and oxygen and/or carbon dioxide from the blood; an outer chamber disposed between the outer wall and the membrane, wherein the plasma which permeates through the membrane enters the outer chamber and exits the filter device via the first outlet; whereby the blood which has been depleted of oxygen and/or carbon dioxide, leukocytes and plasma exists and filter device via the second outlet. 138.-. (canceled)39. A method for processing blood , comprising: a housing comprising an outer wall, a first inlet, a first outlet and a second outlet;', 'a membrane which is capable of separating plasma from said blood, wherein said membrane forms at least one inner chamber within said housing and said blood enters said at least one inner chamber of said blood filter device through said first inlet;', 'a leukocyte, carbon dioxide, and oxygen depletion media disposed within said at least one inner chamber, said leukocyte, carbon dioxide, and oxygen depletion media is capable of depleting leukocytes, carbon dioxide and oxygen from said blood; and', 'an outer chamber disposed between said outer wall and said membrane,, 'passing said blood through a blood filter device comprisingseparating said plasma from said blood by permeating through said membrane, wherein said plasma enters said outer chamber and exits said housing via said first outlet; anddepleting oxygen, leukocytes and plasma from said blood, whereby oxygen, leukocytes and plasma depleted blood exits said housing via said second outlet as packed red blood cells (pRBCs).40. The method according to claim ...

FILTER AND DEVICE INCILUDING THE SAME

A filter and a device including the filter may include a filter and a plurality of pores arranged two-dimensionally on the filter. The plurality of pores may include a plurality of first pores having a longer structure in a certain direction and a plurality of second pores having a longer structure in a direction different from that of the first pore. The first and second pores may have a two-dimensional arrangement in order to suppress or prevent the occurrence of cracks in the filter due to stress. 1. A filter comprising a plurality of pores arranged two-dimensionally to filter a biomaterial ,wherein the plurality of pores comprises:a plurality of first pores extending in a first direction and having a relatively longer structure in the first direction than in a direction perpendicular to the first direction; anda plurality of second pores having a relatively long structure in a second direction different from the first direction,wherein an end portion along a major axis direction of the second pores faces a central portion on a longer side of the first pores.2. The filter of claim 1 , wherein the first and second pores are respectively alternately arranged in the first and second directions.3. The filter of claim 1 , wherein the end portion along the major axis direction of the first pores faces the central portion of the longer side of the second pores or an area adjacent thereto.4. The filter of claim 1 , wherein pairs of some of the first pores are respectively arranged in the first direction with a second pore in between each two pairs claim 1 , the second pore being aligned in the first direction claim 1 , and pairs of some of the first pores are respectively arranged in the second direction with the second pore in between each two pairs.5. The filter of claim 1 , wherein some of the first pores are respectively arranged in the first direction with a second pore in between each two of the first pores claim 1 , the second pore being aligned in the first ...

SEPARATION AND CONCENTRATION OF PARTICLES

Described are devices, methods, and kits for controlled incremental filtration (CIF), as well as methods of designing CIF devices. For example, a method for CIF may modulate a concentration of particles of a desired size in a fluid. The fluid including the particles may be flowed along a flow path through a central channel to contact a plurality of gaps that fluidically couple the central channel to at least one adjacent side channel network. Flow resistance may be decreased along at least a portion of the flow path effective to modulate the concentration of particles. The method may include selecting the plurality of gaps to be larger than the particles. The method may include causing a consistent flow fraction fin the central channel to traverse each gap in the plurality of gaps and flow through the at least one side channel network along the flow path. 1. A controlled incremental filtration (CIF) device , comprising:a. a fluid exchange module in a first substrate, the fluid exchange module comprising a corresponding first microfluidic channel and a first array of island structures in the first microfluidic channel, the first array of island structures being arranged in one or more rows that extend along a longitudinal direction of the first microfluidic channel, each island structure in a row being spaced apart from an adjacent island structure in the row to form an opening,b. wherein the first array of island structures in the fluid exchange module is configured and arranged to shift portions of fluid through the opening between adjacent island structures within a row; anda particle concentration module, the particle concentration module comprising a corresponding second microfluidic channel and a second array of island structures, each island structure in the second array of island structures being spaced apart from an adjacent island structure in the second array of island structures to form an opening,c. wherein the second array of island structures in each ...

Automated System for Processing a Biological Fluid

Systems and methods for processing biological fluids are disclosed. The systems and methods use a reusable hardware unit and a disposable fluid processing circuit that mounts onto the reusable hardware unit. The system and method, under the direction of a pre-programmed controller allow for automatically, opening one or more flow paths to effect addition of one or both parts of an additive solution to a biological fluid component.

SYSTEMS AND METHODS FOR MONITORING AND CONTROLLING FLUID BALANCE DURING A BIOLOGICAL FLUID PROCEDURE

Described is a method for controlling fluid volume balance. A controller is configured with a first set of inputs comprising a hematocrit, a total blood volume, and an ACD ratio. A maximum extracorporeal RBC amount during the procedure is estimated based on the first set of inputs. A fluid circuit is primed with a priming fluid. Whole blood is drawn from a blood source and separated into a RBC component, a target cell component, and a plasma component. The target cell component is directed to a product container. The product container comprising the target cell component is treated. A treated target cell component, a portion of the RBC component remaining in the fluid circuit, and/or a portion of the plasma component remaining in the fluid circuit are returned to the blood source. A first response action is provided if the maximum extracorporeal RBC amount estimated is above a programmed limit. 1. A system for monitoring and controlling fluid balance during an extracorporeal photopheresis procedure , comprising:a disposable fluid circuit comprising a product container configured to receive a target cell component;a separator configured to work in association with the disposable fluid circuit, the separator comprising a chamber configured to rotate about a rotational axis and convey whole blood from a blood source into an inlet region of the chamber for separation into a red blood cell component, a plasma component, and the target cell component; estimate an end-of-procedure fluid balance by calculating a total volume of anticoagulant solution having a citrate concentration to be used for the procedure, wherein the end-of-procedure fluid balance is estimated based on manual or automatic inputs comprising an ACD ratio relating unanticoagulated extracorporeal whole blood to anticoagulant solution, an amount of whole blood to process, a citrate infusion threshold rate, a patient body weight associated with the blood source, and a total blood volume of the blood source ...

SYSTEMS AND METHODS FOR RETURNING TREATED MONONUCLEAR CELLS TO A BLOOD SOURCE

A method for treating mononuclear cells for an extracorporeal photopheresis procedure, driven and adjusted by a microprocessor-based controller, comprising the steps of priming a fluid circuit with priming fluid, directing whole blood derived from a blood source into the fluid circuit, separating the whole blood into a red blood cell component, a mononuclear cell component, and a plasma component, returning a first portion of the red blood cell component and a first portion of the plasma component to the whole blood, adding a photoactivation agent to the mononuclear cell component to create an agent-added mononuclear cell component, irradiating the agent-added mononuclear cell component to create a photoactivated mononuclear cell component, and incubating for a period of time a first portion of the photoactivated mononuclear cell component to create an incubated photoactivated mononuclear cell component. 1. A method for treating mononuclear cells for an extracorporeal photopheresis procedure , driven and adjusted by a microprocessor-based controller , comprising the steps of:priming a fluid circuit with priming fluid;directing whole blood derived from a blood source into the fluid circuit;separating the whole blood into a red blood cell component, a mononuclear cell component, and a plasma component;returning a first portion of the red blood cell component and a first portion of the plasma component to the whole blood;adding a photoactivation agent to the mononuclear cell component to create an agent-added mononuclear cell component;irradiating the agent-added mononuclear cell component to create a photoactivated mononuclear cell component; andincubating for a period of time a first portion of the photoactivated mononuclear cell component to create an incubated photoactivated mononuclear cell component.2. The method of claim 1 , further comprising:retaining a second portion of the red blood cell component and a second portion of the plasma component within the fluid ...

Systems, Devices, and Methods For Thrombolysis

Disclosed herein are systems, devices, and methods for thrombolysis. For example, a medical device for thrombolysis can include a conduit, a supply reservoir, and a waste reservoir. The conduit can be configured to insert into a lumen of a venous access device having an intraluminal clot. The conduit can include a supply lumen configured to convey an aqueous thrombolytic composition from the supply reservoir through an opening in a distal-end portion of the conduit to the intraluminal clot. The waste reservoir can be configured to collect waste from the lumen of the venous access device including fibrin fragments, platelets, red blood cells, or spent solution of the thrombolytic composition used to break down the intraluminal clot. Administering a thrombolytic composition in accordance with the disclosed systems, devices, and methods can break down clots more quickly than at least the common 3-way stopcock method. 1. A medical device for thrombolysis , comprising:a conduit configured to insert into a lumen of a venous access device, the conduit including a supply lumen configured to convey an aqueous thrombolytic composition from a supply reservoir thereof through an opening in a distal-end portion of the conduit for administration of the thrombolytic composition to an intraluminal clot in the lumen of the venous access device;a connector coupled to a proximal-end portion of the conduit, the connector configured to fluidly connect the supply reservoir to the supply lumen of the conduit; anda waste reservoir configured to fluidly connect to the lumen of the venous access device, the waste reservoir configured to collect waste from the lumen of the venous access device including fibrin fragments, platelets, red blood cells, or spent solution of the thrombolytic composition used to break down the intraluminal clot in the lumen of the venous access device.2. The medical device of claim 1 , further comprising the supply reservoir optionally pre-filled with the ...

Biological component collection kit and blood component collection system

A third cassette ( 28 ) of a blood component collection kit ( 12 ) is equipped with a third cassette main body ( 29 ) in which a flow path (F) is formed together with having a filter accommodating unit ( 86 ), and a filter member ( 88 ) disposed in the filter accommodating unit ( 86 ). The flow path (F) includes a red blood cell line (f 1 ) in which red blood cells flow, a preservation solution line (f 2 ) in which a preservation solution flows, a confluence section (f 3 ) in which the red blood cells and a preservation solution are made to merge together, a mixed liquid line (f 4 ) through which a mixed liquid of the red blood cells and the preservation solution flows, and a filter chamber (f 5 ) formed inside the filter accommodating unit ( 86 ).

Method of Blood Pooling and Storage

The disclosure provides methods of making a cell-containing product having a uniform amount of cells therein. The method comprises pooling red blood cells from a plurality of blood units, and inactivating any pathogen contained therein. A storage solution added to the cellular component results in a cell-containing product that is essentially pathogen and white blood cell free and has an extended shelf life of about 42 to about 100 days. The cell-containing product is further divided into units which comprise a uniform dose of RBCs per unit.

MEMBRANE SEPARATION DEVICES, SYSTEMS AND METHODS EMPLOYING SAME, AND DATA MANAGEMENT SYSTEMS AND METHODS

A membrane separation device is disclosed along with systems and methods employing the device in blood processing procedures. In one embodiment, a spinning membrane separator is provided in which at least two zones or regions are created in the gap between the spinning membrane and the shell, such that mixing of the fluid between the two regions is inhibited by a radial rib or ridge associated with the spinning membrane that decreases the gap between the spinning membrane and the shell to define two fluid regions, the ridge isolating the fluid in the two regions to minimize mixing between the two. Automated systems and methods are disclosed for separating a unit of previously-collected whole blood into selected blood components, such as concentrated red cells and plasma, for collecting red cells and plasma directly from a donor in a single pass, and for cell washing. Data management systems and methods and priming methods are also disclosed. 154.-. (canceled)55. A disposable fluid circuit configured to interface with a hardware component to form an automated blood collection system for collecting red blood cells and plasma from a donor comprising , the disposable component comprising:a donor access device for withdrawing whole blood from a donor;a whole blood collection container in communication with the donor access device;a blood separation device communicating with the whole blood collection container and employing relatively rotating surfaces, at least one of which carries a membrane substantially permeable to plasma and substantially impermeable to red blood cells to separate the whole blood into substantially concentrated red cells and plasma;a first collection container communicating with the blood separation device for receipt of the substantially concentrated red blood cells;a source of preservative solution communicating with the first collection container; anda second collection container communicating with the blood separation chamber for receipt of the ...

LOW VOLUME EXTRACORPOREAL PHOTOPHERESIS SYSTEMS AND METHODS

Systems and methods for performing low volume (e.g., 500 mL or less) extracorporeal photopheresis (ECP) procedures are disclosed. Each of the different systems and methods eliminates the need for multiple kits and solutions and reduce some of the potential risks inherent in the use of such multiple kits and solutions. 1. A unitary disposable kit for performing low volume extracorporeal photopheresis comprising:a) a first container configured to receive whole blood from a patient and to be mountable in both a centrifuge and a blood component separator;b) a second container connected to the upper portion of the first container by a first tubing segment for receipt of plasma configured to be mountable in both a centrifuge and a blood component separator, the first container and second container each having an upper portion and a lower portion when mounted in the centrifuge and blood component separator;c) a third container connected to the lower end of the first container by a second tubing segment for receipt of packed red blood cells;d) a fourth container connected to the first container by a third tubing segment for receipt of buffy coat and configured to be mountable in an irradiation device.2. The unitary disposable kit of further comprising a phlebotomy needle connected to the first container by a fourth tubing segment for introducing whole blood into the first container and wherein the first container is prefilled with a volume of anticoagulant.3. The unitary disposable kit of further comprising a fifth tubing segment connected to the fourth container for introducing a photoactivation agent into the fourth container.4. The unitary disposable kit of further comprising a fifth container containing prefilled with a volume of photoactivation agent and connected to the fourth container by the fifth tubing segment.5. The unitary disposable kit of further comprising a sixth container prefilled with a volume of saline and connected to the first container by a sixth ...

Interface Detector For Blood Processing System

Blood separation systems and methods are provided for controlling the interface between separated blood components. The system includes a centrifuge assembly having a light-transmissive portion, a light reflector, and a fluid processing region therebetween. An optical sensor system emits a scanning light beam along a path toward the light-transmissive portion, which transmits at least a portion of the scanning light beam to the fluid processing region and the light reflector. The light reflector reflects at least a portion of the scanning light beam toward the optical sensor system along a path substantially coaxial to the path of the scanning light beam from the optical sensor system toward the light-transmissive portion of the centrifuge assembly. The scanning light beam may be a white light beam or narrow spectrum beam. The reflected beam may be directed through the optical sensor system via optical fibers. 117-. (canceled)18. An optical sensor system for use in combination with a blood processing system , the optical sensor system comprising:a light source;a light detector; andan optical fiber providing a light path between the light source and the light detector.19. The optical sensor system of claim 18 , further comprising a housing claim 18 , wherein the light source is at least partially positioned within the housing claim 18 , the light detector is positioned outside of the housing claim 18 , and the optical fiber is connected to the housing.20. The optical sensor system of claim 19 , wherein the optical fiber is adjustably connected to the housing.21. The optical sensor system of claim 19 , further comprising a plurality of optical fibers connected to the housing by an adjustable module configured to simultaneously adjust the position of the optical fibers with respect to the housing.22. The optical sensor system of claim 18 , further comprising a beam splitter configured to receive light from the light source and direct at least a portion of the light ...

Interface Detector For Blood Processing System

Blood separation systems and methods are provided for controlling the interface between separated blood components. The system includes a centrifuge assembly having a light-transmissive portion, a light reflector, and a fluid processing region therebetween. An optical sensor system emits a scanning light beam along a path toward the light-transmissive portion, which transmits at least a portion of the scanning light beam to the fluid processing region and the light reflector. The light reflector reflects at least a portion of the scanning light beam toward the optical sensor system along a path substantially coaxial to the path of the scanning light beam from the optical sensor system toward the light-transmissive portion of the centrifuge assembly. The scanning light beam may be a white light beam or narrow spectrum beam. The reflected beam may be directed through the optical sensor system via optical fibers. 141-. (canceled)42. An optical sensor system for use in combination with a blood processing system , the optical sensor system comprising a white light source.43. The optical sensor system of claim 42 , wherein the white light source comprises a light-emitting diode.44. The optical sensor system of claim 42 , wherein the white light source has a relatively high spectral power distribution in the red wavelength spectrum.45. The optical sensor system of claim 42 , wherein the white light source has a relatively high spectral power distribution in the blue wavelength spectrum.46. The optical sensor system of claim 42 , further comprising a light detector positioned adjacent to the white light source and configured to monitor the intensity of light emitted by the white light source.47. A blood processing system claim 42 , comprising:a centrifuge assembly including a light-transmissive portion and a fluid processing region positioned at least partially adjacent to the light-transmissive portion; andan optical sensor system that emits a white light directed toward ...

SYSTEMS AND METHODS FOR PLATELET CONCENTRATION WITH A SPINNING MEMBRANE SEPARATOR

A method for automated processing of a blood product, the method comprising providing a reusable separation apparatus controlled by a microprocessing unit, said apparatus configurable with settings and configured to associate with a disposable circuit comprising a separator and in communication with a source blood product having a first concentration and first volume. The apparatus and disposable circuit are configured to flow the source blood product into an inlet of the separator and separate supernatant of the source blood product from a first outlet of the separator into a filtrate container. The apparatus and disposable circuit are also configured to separate platelets and remaining supernatant from a second outlet of the separator into a retentate container, wherein the platelets and remaining supernatant in the retentate container have a second concentration greater than the first concentration and second volume less than the first volume. 1. A system for automated processing of a blood product , the system comprising:a reusable separation apparatus controlled by a microprocessing controller unit driven by software, wherein the apparatus and microprocessing controller unit are configurable with a plurality of settings;a disposable sterile circuit configured to associate with the reusable separation apparatus, the disposable sterile circuit comprising a spinning membrane separator comprising a porous membrane, wherein the separator comprises first and second outlets and an inlet configured to communicate with a source blood product having a first concentration and first volume;wherein the separation apparatus is configured by the microprocessing controller to flow the source blood product into the inlet of the separator, separate an amount of supernatant of the source blood product from the first outlet into a filtrate container, and separate platelets and remaining supernatant from the second outlet into a retentate container;wherein the separation apparatus ...

APPARATUS FOR EXTRACTING A BLOOD COMPONENT CONTAINED IN A SYSTEM OF BAGS

An apparatus for extracting at least one blood component contained in a primary bag of a system of bags, said system of bags comprising at least one bag of solution containing an additive solution for said blood component and at least one collecting satellite bag configured to collect said blood component to which said additive solution has been added, said bags being connected to one another by tubes, said apparatus comprising an extraction device configured to extract the blood component by compressing the primary bag, and a control device configured to transfer said extracted component or of said extracted component and of the additive solution, to the collecting satellite bag by the intermediary of tubes, further comprising: an agitation device, and a drive system, said system being designed to enable the uniform mixing of the extracted blood component and of the additive solution in said collecting satellite bag. 119-. (canceled)20. An apparatus for extracting at least one blood component contained in a primary bag of a system of bags , said system of bags further comprising at least one bag of solution containing an additive solution for said blood component and at least one collecting satellite bag configured to collect said blood component to which said additive solution has been added , said bags being connected to one another by tubes , said apparatus comprising:an extraction device configured to extract the blood component by compressing the primary bag, anda control device configured to transfer said extracted component or said extracted component and the additive solution, to the collecting satellite bag by the intermediary of tubes, further comprising:an agitation device configured to agitate the collecting satellite bag, anda drive system configured to control said extraction device, said control device and said agitation device, said system being designed to enable the uniform mixing of the extracted blood component and of the additive solution in ...

Membrane separation and washing devices, systems and methods employing same, and data management systems and methods

A method of separating blood into two or more components and subsequently washing a component, comprising providing a blood storage container containing an initial blood composition, and a blood separation circuit comprising a separator. The method also comprises separating and washing cellular material within the same blood separation circuit.

Method and system for collecting leukoreduced red blood cells

A method and system for collecting leukoreduced red blood cells employing a spinning membrane separator including a housing having an upper end region and a lower end region in an operating position with a red blood cell outlet in the upper end region of the housing and a whole blood inlet in the lower end region of the housing. The method and system provide for flowing additive solution into the whole blood inlet of the housing to prime the separator; flowing whole blood into the whole blood inlet of the housing separating red blood cells from the whole blood; flowing separated red blood cells out of the red blood cell outlet of the housing; combining the separated red blood cells with additive solution: passing the separated red blood cells and additive solution combination through a leukoreduction filter; and collecting the filtered red blood cells and additive solution. 158-. (canceled)59. A fluid processing circuit for the collection of leukoreduced red blood cells , comprising:a blood separator for separating red blood cells from whole blood wherein the separator includes a membrane configured to spin about a generally vertically-oriented axis within a housing, the housing including an upper end region and a lower end region in an operating position, and the separator including a red blood cell outlet in the upper end region of the housing and a whole blood inlet in the lower end region of the housing;a source of additive solution in communication with the whole blood inlet for priming the blood separator with additive solution;a red blood cell flow path connecting a leukoreduction filter to the red blood cell outlet;a red blood cell collection container connected to the leukoreduction filter for collecting red blood cells after passage through the leukoreduction filter; andthe source of additive solution being in communication with the red blood cell flow path upstream of the leukoreduction filter wherein additive solution is added to the red blood cell flow ...

MEDICATION INFUSION DEVICES, SYSTEMS, AND METHODS

Devices, systems, and methods for medication infusion are described herein. In some embodiments, a system includes a patient access subassembly, a first fluid reservoir, a second fluid reservoir, and an assembly. The assembly can have a first configuration in which the patient access subassembly is in fluid communication with the first fluid reservoir via a first tube, a second configuration in which the first fluid reservoir is in fluid communication with the second fluid reservoir, and a third configuration in which the first fluid reservoir is in fluid communication with the patient access subassembly via a second tube, the first fluid reservoir fluidically isolated from the first tube in the third configuration. 1. A method , comprising:coupling a patient access subassembly to a patient, the patient access subassembly fluidically coupled to a first fluid reservoir containing a first substance and a second fluid reservoir containing a second substance via an assembly;drawing cells through the patient access subassembly, through the assembly, and into the first fluid reservoir such that the cells and the first substance form a third substance;manipulating the assembly such that the first fluid reservoir is fluidically isolated from the patient access subassembly and such that the first fluid reservoir is in fluidic communication with the second fluid reservoir;transferring a portion of the third substance from the first fluid reservoir through the assembly and into the second fluid reservoir such that the portion of the third substance and the second substance form a fourth substance;transferring the fourth substance from the second fluid reservoir through the assembly and into the first fluid reservoir such that the remainder of the third substance and the fourth substance form a fifth substance;manipulating the assembly such that the first fluid reservoir is in fluid communication with the patient access subassembly;transferring the fifth substance from the ...

Systems Enabling Alternative Approaches To Therapeutic Red Blood Cell Exchange And/Or Therapeutic Plasma Exchange

Systems and methods are provided for therapeutic red blood cell exchange and/or therapeutic plasma exchange. A blood separation device includes a centrifugal separator, a spinning membrane separator drive unit, a pump system, and a controller. Blood is conveyed through a fluid flow circuit into either the centrifugal separator or the spinning membrane separator, which separates out the target blood component (red blood cells, in the case of therapeutic red blood cell exchange, or plasma, in the case of therapeutic plasma exchange). The target blood component is retained in the circuit as a waste product, while a replacement fluid is added to the remaining blood component(s), which is then conveyed to a recipient. In addition to allowing for execution of an exchange procedure using either a centrifugal separator or a spinning membrane separator drive unit, the blood separation device also allows for the use of differently sized spinning membrane separators.

System for Recovering Autologous Thrombin

A system is disclosed for recovering autologous thrombin from a blood product. The blood product is drawn into an injecting syringe and a clot is allowed to form. A discharge port of the injecting syringe is communicably connected to an intake port of an aspirating syringe through a hydrophilic filter assembly. The injecting and aspirating syringes are simultaneously operated to transmit blood product through the filter such that fibrous clotting material and red blood cells are removed by the filter and a concentrated, high-quality thrombin solution is recovered. 1. An autologous thrombin recovery system comprising:an injecting syringe including a supply receptacle for holding a blood product and having a discharge port formed therein, said injecting syringe further including a first piston assembly operatively interengaged with said supply receptacle;an aspirating syringe including a receiving receptacle having a fluid inlet port formed therein and a second piston assembly operatively engaged with said receiving receptacle; anda filter assembly operably interconnected between said injecting and aspirating syringes, said filter assembly including a fluid intake port for communicably connecting to said discharge port of said injecting receptacle and a fluid outlet port for communicably connecting to said inlet port of said aspirating syringe, said filter assembly further including a filter component disposed between and communicably interconnecting said intake and outlet ports of said filter assembly; said first piston assembly of said injecting syringe being driven through said supply receptacle of said injecting syringe and said second piston assembly being simultaneously retracted through said receiving receptacle of said aspirating syringe for transmitting the blood product from said supply receptacle of said injecting syringe through said filter component of said filter assembly such that constituent components of the blood product are removed by said filter ...

Container for rapid blood detection and blood loss assessment

Disclosed are methods, materials and devices for approximation of blood volume in a fluid, such as in a biological fluid collected during a surgical procedure. The method and devices include the use of a RBC flocculant, such as polyDADMAC, and an approximate blood hematocrit for the type of animal, as well as a calculated RBC packing ratio corresponding to the collection device being used. Also provided is a Blood Indicator Panel (BIP), comprising a series of markings calculated from an observed red blood settlement volume, the average animal type hematocrit, and a calculated RBC packing ratio “η” value for the collection device. Pediatric (about 200 ml or 250 ml size container), adult human (about 1,000 ml-1,500 ml) and veterinary (about 500 ml-2,500 ml) collection containers are also disclosed, that include a RBC flocculant, for use in approximating blood volume in a fluid.

Custom data fields for automated apheresis procedures

A method and automated system for processing blood in which the automated system includes a programmable controller, a database, and an interactive display screen for displaying information and receiving operator input. The programmable controller is configured to automatically control the system to perform the method. Upon activation of the system, the screen displays a listing of different blood processing procedures that may be performed using the system. The operator may then input into the controller an identification of a specified blood processing procedure that is to be performed, such that an initial list of parameters that are associated with the specified blood processing procedure are displayed on the screen. The operator may then input into the controller an identification of the parameters that are to populate the display screen during performance of the procedure and indicate a format in which the selected parameters are to be presented on the display screen. The controller then creates a display for the specified blood processing procedure. Current values of the selected parameters in the selected format are displayed on the screen during performance of the specified procedure. The controller automatically saves an image of the display screen periodically during performance of the specified blood processing procedure, and transfers information from the saved images of the display screens to a procedure record form.

Cell washing device using a bulk acoustic wave with phantom material

Separation devices for separating a component from a multicomponent mixture are provided. The separation devices can include a body that defines a separation channel with a cross-sectional geometry that is not circular, rectangular, or square. The separation devices can be positioned relative to a base having an acoustic wave generator. A standing bulk acoustic wave generated by the acoustic wave generator can separate one or more components from the multicomponent mixture. Methods are provided for separating one or more components from a multicomponent mixture using the separation devices.

FUNCTIONALLY-CLOSED, STERILE BLOOD PROCESSING SOLUTION SYSTEM AND METHOD

Delivering a blood processing solution to blood in a blood bag includes coupling a first tube to a vented spike at one end and to a Y-shaped tube connector at a second end. An in-line microbiotic barrier filter is coupled to the first tube between its ends. A second tube is coupled to a transfer bag at one end and to the Y-shaped tube connector at its other end. A third tube is coupled to the output of the Y-shaped tube connector and sealed at its distal end. The blood bag includes a fourth tube that is sealed at a distal end. The third tube is welded to the fourth tube using a sterile tubing welder, wherein a functionally-closed, sterile flow path through which the blood processing solution can flow into the blood bag is maintained. 120-. (canceled)21. A functionally-closed , sterile Y-type tube set comprising:a vented spike coupled to a first end of a first tube;a Y-shaped tube connector having a first and a second input and an output, wherein a second end of the first tube is coupled to the first input of the Y-shaped tube connector;an in-line microbiotic barrier filter coupled between the first and second ends of the first tube, wherein a flow path extends from the first end to the second end of the first tube that passes through the microbiotic barrier filter;a transfer bag coupled to a first end of a second tube, wherein a second end of the second tube is coupled to the second input of the Y-shaped tube connector; anda third tube coupled to the output of the Y-shaped tube connector, wherein a second end of the third tube is sealed.22. The functionally-closed claim 21 , sterile Y-type tube set of claim 21 , wherein the inline microbiotic filter is a 0.2 micron IV filter.23. A method for delivering a blood processing solution to blood present in a blood bag claim 21 , the method comprising:obtaining a functionally-closed sterile Y-type tube set comprising an input tubing member coupled to an output tubing member and providing a sterile flow path extending from a ...

MEDICAL APPARATUS FOR EXTRACORPOREAL BLOOD TREATMENT AND METHOD FOR DETERMINING A BLOOD PARAMETER VALUE IN A MEDICAL APPARATUS THEREOF

A medical apparatus for extracorporeal blood treatment and a method for determining blood parameter value have been provided. The medical apparatus has a control unit () configured for taking from a storage memory () a plurality of blood parameter value measures each made through the sensors () provided on the bloodline set of each of a machines plurality at different patient treatment sessions; all the blood parameter values relate to the same patient; receiving at least a corresponding laboratory measured valise of the same blood parameter relating to the same patient and a laboratory measurement time; determining a correction factor function of a difference between a measured value made though the sensor and a laboratory measured value; obtaining an actual value of said blood parameter by varying at least the last measure made through the sensor by means of the correcting factor. 29. Medical apparatus according to claim 1 , wherein the control unit () is configured for obtaining a hemoglobin value by varying at least the last measure made through the sensor by means of the correcting factor.39. Medical apparatus according to claim 1 , wherein the control unit () is configured for performing the following steps:{'b': 8', '12, 'interpolating said plurality of measures made through the hematocrit sensor () for obtaining an interpolated hemoglobin curve () defining a hemoglobin trend along time;'}determining the correcting factor as a difference between the actual control hemoglobin value and a time-corresponding hemoglobin value in the interpolated curve.49. Medical apparatus according to claim 3 , wherein the control unit () is further configured for performing the following steps:{'b': 12', '12', '12', '12, 'i': a', 'a, 'translating at least the last part () of the interpolated curve () by means of the correcting factor, the last part () of the interpolated curve () comprising at least the last two measured hemoglobin values;'}{'b': '13', 'displaying either the at ...

SYSTEM AND METHOD TO LYSE AND REMOVE RED BLOOD CELLS FROM A CELL PRODUCT

A method is provided for removing red blood cells from a suspension comprising red blood cells, white blood cells, platelets and plasma using a spinning membrane separator. The method comprises: a) flowing whole blood into the gap of the spinning membrane separator; b) collecting red blood cells and white blood cells in the gap and passing plasma and platelets through the membrane; c) introducing a first quantity of lysing buffer into the gap; d) incubating the red blood cells, white blood cells and lysing buffer in the gap for a period of time to cause a lysis reaction with the red blood cells; e) introducing a second quantity of lysing buffer into the gap to displace the first quantity of lysing buffer and a first quantity of red blood cell debris out of the gap; f) introducing a first quantity of wash buffer into the gap to quench the lysis reaction and displace the second quantity of lysing buffer and a second quantity of red blood cell debris out of the gap; and g) introducing a second quantity of wash buffer into the gap to flow washed white blood cells out of the housing. 1. A method for removing red blood cells from a suspension comprising red blood cells , white blood cells , platelets and plasma using a spinning membrane separator , the spinning membrane separator comprising a housing , a rotor rotatably supported within the housing , a membrane affixed to the rotor having a porous surface that permits plasma and platelets to pass therethrough but not red blood cells and white blood cells , with a gap being defined between the housing and the membrane , the method comprising:a) flowing whole blood into the gap;b) collecting red blood cells and white blood cells in the gap and passing plasma and platelets through the membrane;c) introducing a first quantity of lysing buffer into the gap;d) incubating the red blood cells, white blood cells and lysing buffer in the gap for a period of time to cause a lysis reaction with the red blood cells;e) introducing a ...

MEMBRANE SEPARATION DEVICES, SYSTEMS AND METHODS EMPLOYING SAME, AND DATA MANAGEMENT SYSTEMS AND METHODS

A membrane separation device is disclosed along with systems and methods employing the device in blood processing procedures. In one embodiment, a spinning membrane separator is provided in which at least two zones or regions are created in the gap between the spinning membrane and the shell, such that mixing of the fluid between the two regions is inhibited by a radial rib or ridge associated with the spinning membrane that decreases the gap between the spinning membrane and the shell to define two fluid regions, the ridge isolating the fluid in the two regions to minimize mixing between the two. Automated systems and methods are disclosed for separating a unit of previously-collected whole blood into selected blood components, such as concentrated red cells and plasma, for collecting red cells and plasma directly from a donor in a single pass, and for cell washing. Data management systems and methods and priming methods are also disclosed. 133-. (canceled)34. A blood processing system , comprising:a leukoreduction filter for removing leukocytes from red blood cells as the red blood cells pass through the filter; anda pump for pumping an additive solution through the leukoreduction filter to flush the leukoreduction filter of red blood cells that remain in the leukoreduction filter after filtration of the red blood cells, wherein the pump increases the flush rate of the additive solution during flushing of the leukoreduction filter.35. (canceled)36. The system of wherein the pump gradually increases the flush rate step-wise.37. The system of wherein the pump continuously increases the flush rate claim 34 ,38. The system of wherein the pump increases the flush rate depending on one or more of hematocrit of the red blood cells within the filter claim 34 , the amount of hemolysis of the red blood cells within the filter and a pressure within the filter.39. The system of wherein the pump increases the flush rate based on real-time measurements.40. The system of wherein ...

Passive Separation of Whole Blood

Described are systems, methods, and kits for compression sedimentation and whole blood separation. For example, a compression sedimentation system may include a compression stage configured to accept a flexible reservoir configured to contain a liquid mixture. The compression stage may include a base substrate and a compression substrate configured to apply a force to the flexible reservoir effective to create a pressure in the liquid mixture. An apparatus for whole blood separation may include a sedimentation system that separates whole blood into a supernatant including platelet rich plasma and a subnatant including red blood cells. At least one platelet-concentrating device may be included to receive the supernatant including the PRP and to separate a platelet concentrate and a platelet poor plasma from the supernatant.

Method of estimating blood volume

Disclosed are methods, materials and devices for approximation of blood volume in a fluid, such as in a biological fluid collected during a surgical procedure. The method and devices include the use of a RBC flocculant, such as polyDADMAC, and an approximate blood hematocrit for the type of animal, as well as a calculated RBC packing ratio corresponding to the collection device being used. Also provided is a Blood Indicator Panel (BIP), comprising a series of markings calculated from an observed red blood settlement volume, the average animal type hematocrit, and a calculated RBC packing ratio “η” value for the collection device. Pediatric (about 200 ml or 250 ml size container), adult human (about 1,000 ml-1,500 ml) and veterinary (about 500 ml-2,500 ml) collection containers are also disclosed, that include a RBC flocculant, for use in approximating blood volume in a fluid.

Systems and methods for preservation of cells

Provided herein are systems and methods for molecule delivery into cells. The method may include steps of forming a solution comprising an amount of microbubbles, an amount of soluble molecules, and one or more cells, flowing the solution through a microfluidic device, and sonicating the solution for a period of time sufficient to form pores in the one or more cells. In particular, such methods may be used for preservation of cells through delivery of a preservation agent.

CELL WASHING USING ACOUSTIC WAVES

Disclosed is a device for separating a cellular component from a multicomponent fluid. The device can comprise a body, a first acoustic wave generator, and a second acoustic wave propagating component. The body can define a channel having a first surface and a second surface opposite the first surface. The channel can extend along a longitudinal axis from a first end to a second end. The first acoustic wave generator can be coupled to the first surface. The first acoustic wave generator can be configured to generate an acoustic wave having a wavelength. The second acoustic wave propagating component can be coupled to the second surface. The second surface can be spaced an integer fractional multiple of the wavelength from the first surface and each integer factional multiple equals a number of pressure nodes within the channel. 1. A device for separating a cellular component from a multicomponent fluid , the device-comprising:a body defining a channel having a first surface and a second surface opposite the first surface, the channel extending along a longitudinal axis from a first end to a second end;a first acoustic wave generator coupled to the first surface, the first acoustic wave generator configured to generate an acoustic wave having a wavelength; anda second acoustic wave propagating component coupled to the second surface,wherein the second surface is spaced an integer fractional multiple of the wavelength from the first surface and each factional multiple equals a number of pressure nodes within the channel.2. The device of claim 1 , wherein a central power generating region of the first acoustic wave generator is aligned with the second end of the channel and proximate a bifurcation region of the channel.3. The device of claim 1 , wherein the integer fractional multiple is 0.5 and the number of pressure nodes is 1.4. The device of claim 1 , wherein the first acoustic wave generator and the second wave propagating component are located proximate a ...

SINGLE COLLECTION BAG BLOOD COLLECTION SYSTEM, METHOD AND APPARATUS

A blood or blood component collection and processing system, apparatus and method are disclosed. One embodiment includes a vascular access device and a blood collection container having a blood inlet and first and second blood component outlets. The outlets are located at opposite ends of the container, and a blood flow conduit extends between the vascular access device and collection container. The collection container is free of attachment to other blood collection containers when in an initial collection configuration at the time of collection. 120-. (canceled)21. A red cell collection system comprising:a red cell collection assembly comprising:a red cell collection container,a red cell flow conduit having one end defining a red cell inlet and another end in fluid communication with the red cell collection container, anda tubing loop separate from the red cell flow conduit and having one end in fluid communication with the interior of the red cell container and another end in fluid communication with red cell flow conduit at a junction between the ends of the red cell flow conduit,the red cell flow conduit and the loop configured for cooperation with a pump for promoting mixing of the red blood cells.22. The red cell collection system of claim 21 , wherein the red cell flow conduit comprises a leukocyte filter disposed between the junction and the red cell collection container.23. The red cell collection system of claim 21 , wherein the red cell collection assembly comprises a processing container connected to and in fluid communication with the red cell flow conduit upstream of the junction.24. The red cell collection system of claim 23 , in which the red cell collection container contains certain ingredients of a red cell preservative solution and the processing container contains other ingredients of the red cell preservative solution.25. The red cell collection system of claim 24 , in which the red cell collection container contains glucose and the processing ...

Blood component separation device

A blood reservoir ( 20 ) for storing blood includes a first storage section ( 21 ), a second storage section ( 22 ), and a third storage section ( 23 ) that is provided between the first storage section and the second storage section, and communicates with the first storage section and the second storage section. A volume of the blood reservoir ( 20 ) can be adjusted by changing the amount of expansion or contraction of a bellows structure ( 28 ) using a bellows adjustment mechanism ( 83, 93 ).

BLOOD COMPONENT SAMPLING CASSETTE, BLOOD SAMPLING CIRCUIT SET, AND BLOOD COMPONENT SAMPLING SYSTEM

A blood component sampling cassette which can be more efficiently manufactured at lower cost as compared to a typical cassette, a blood sampling circuit set, and a blood component sampling system. A blood component sampling cassette () includes a cassette main body () made of a soft material to which heat sterilization is applicable. The cassette main body () is provided with a retransfusion line (). The retransfusion line () is provided with a reservoir () configured to temporarily store a blood component to be returned to a blood donor. The reservoir () is pressed by a retransfusion pump () to discharge the blood component from the reservoir (). 1. A biological component sampling cassette including a cassette main body provided with a plurality of flow paths and configured to be attachable to a separation device configured to separate a biological component from liquid containing at least one biological component ,wherein the cassette main body is consists exclusively of a first sheet of a soft material to which heat sterilization is applicable and a second sheet of said soft material, said first and second sheet being bonded to each other to form said plurality of flow paths, an introduction line configured to introduce the liquid,', 'a biological component transfer line configured to transfer, to a sampling container, the biological component obtained by separation processing of the liquid, and', 'a return line configured to transfer liquid other than the biological component obtained by the separation processing,, 'the plurality of flow paths includes'}the return line is provided with a reservoir configured to temporarily store the liquid to be returned, andthe reservoir is configured to be expandable and contractable, and is pressed by a return pump provided at the separation device to discharge the liquid from the reservoir.2. The biological component sampling cassette according to claim 1 , whereinthe cassette main body has a sensor pressing section ...

SYSTEM AND METHOD FOR CONTINUOUS FLOW RED BLOOD CELL WASHING

A method for continuously washing packed red blood cells includes (1) transferring, at a first flow rate, packed red blood cells from a container to a separation device and (2) transferring, at the same time as the packed red blood cells, wash solution from a container to the separation device. The wash solution may be transferred at a second flow rate that is greater than the first flow rate. The wash solution mixes with the packed red blood cells within the inlet line of the separation device and dilutes/washes the packed red blood cells. The separation device separates the red blood cells from the wash solution and a supernatant. The method may then monitor the volume of washed red blood cells within the separation device and begin to extract the washed red blood cells into a red blood cell product container when a target volume is collected within the separation device. 1. A method for continuously washing packed red blood cells comprising:(a) connecting a wash solution container to a wash solution line, the wash solution line fluidly connecting the wash solution container and an inlet line of a blood component separation device;(b) connecting a red blood cell container to a red blood cell line, the red blood cell line fluidly connecting the red blood cell container and the inlet line of the blood component separation device, the red blood cell container containing a volume of packed red blood cells;(c) transferring, at a first flow rate, packed red blood cells from the red blood cell container to the blood component separation device;(d) transferring, at the same time as step (c), wash solution from the wash solution container to the blood component separation device, the wash solution being transferred at a second flow rate that is greater than the first flow rate, the wash solution mixing with the packed red blood cells in the inlet line, thereby diluting and washing the packed red blood cells, the blood component separation device separating washed red blood ...

System and Method for Washing Shed Blood

A method for collecting and washing shed blood includes providing a blood salvage reservoir and a blood salvage system. The user may connect a vacuum inlet port on the reservoir to a vacuum outlet port on the salvage system, connect an inlet port on the reservoir to a patient, and connect a vacuum source to a vacuum connection port on the salvage system. The method may then (1) draw a vacuum on the reservoir to draw shed blood into the reservoir, (2) disconnect the reservoir from the surgical field and salvage system, and (3) connect a second reservoir. The first blood salvage reservoir may then be connected to an automated blood processing system, and the collected blood may be introducing into the automated blood processing system and washed. The blood processing system may then return a portion of the washed blood to the patient. 1. A method for collecting and washing shed blood comprising:providing a first blood salvage reservoir having an inlet port, an outlet port and a vacuum inlet port;providing a blood salvage system having a vacuum connection port and a vacuum outlet port, the vacuum inlet port on the first blood salvage reservoir fluidly connected to the vacuum outlet port on the blood salvage system;fluidly connecting the inlet port of the first blood salvage reservoir to a surgical field of a patient, the inlet port configured to allow blood lost during a surgical procedure to be collected within the first blood salvage reservoir;fluidly connecting the vacuum connection port on the blood salvage system to a vacuum source;drawing a vacuum on the first blood salvage reservoir and the blood salvage system to draw blood from the surgical field and into the blood salvage reservoir;collecting a volume of shed blood within the first blood salvage reservoir;disconnecting the first blood salvage reservoir from the surgical field and from the blood salvage system;fluidly connecting a second blood salvage reservoir to the surgical field and blood salvage system to ...

METHOD OF BLOOD POOLING AND STORAGE

The disclosure provides methods of making a cell-containing product having a uniform amount of cells therein. The method comprises pooling red blood cells from a plurality of blood units, and inactivating any pathogen contained therein. A storage solution added to the cellular component results in a cell-containing product that is essentially pathogen and white blood cell free and has an extended shelf life of about 42 to about 100 days. The cell-containing product is further divided into units which comprise a uniform dose of RBCs per unit. 1. A method for preparing uniform dose blood components from a plurality of whole blood units of the same blood group and type comprising:a) leukoreducing a plurality of individual whole blood units to form leukoreduced blood components, wherein the leukoreduced blood components comprises RBCs, platelets, and plasma, wherein the plurality of whole blood units comprises 10-100 whole blood units;b) pooling the leukoreduced blood components from the plurality of whole blood units;c) treating the pooled leukoreduced blood component to inactivate one or more pathogens;d) separating at least one of a pooled RBC component, a pooled platelet component, and a pooled plasma component from the pooled leukoreduced blood component; ande) dividing at least one of the pooled RBC component, pooled platelet component, and pooled plasma component into uniform dose product units.2. The method of claim 1 , wherein the blood component is separated into at least two claim 1 , or all three claim 1 , of RBCs claim 1 , platelets claim 1 , and plasma from the leukoreduced blood component.3. The method of claim 1 , further comprising adding a storage solution to the pooled RBC component or the pooled platelet component after the separating step and before the dividing step.4. The method of claim 3 , wherein the storage solution comprises at least one material selected from the group consisting of adenine claim 3 , glucose claim 3 , phosphate claim 3 , ...

CUSTOM DATA FIELDS FOR AUTOMATED APHERESIS PROCEDURES

A method and automated system for processing blood in which the automated system includes a programmable controller, a database, and an interactive display screen for displaying information and receiving operator input. The programmable controller is configured to automatically control the system to perform the method. Upon activation of the system, the screen displays a listing of different blood processing procedures that may be performed using the system. The operator may then input into the controller an identification of a specified blood processing procedure that is to be performed, such that an initial list of parameters that are associated with the specified blood processing procedure are displayed on the screen. The operator may then input into the controller an identification of the parameters that are to populate the display screen during performance of the procedure and indicate a format in which the selected parameters are to be presented on the display screen. The controller then creates a display for the specified blood processing procedure. Current values of the selected parameters in the selected format are displayed on the screen during performance of the specified procedure. The controller automatically saves an image of the display screen periodically during performance of the specified blood processing procedure, and transfers information from the saved images of the display screens to a procedure record form. 1. A method for processing blood or blood products using an automated system including a programmable controller , a database , and an interactive display screen for displaying information and receiving operator input , the method comprising:a) upon activation of the system, displaying on the screen a listing of different blood processing procedures that may be performed using the system;b) upon inputting into the controller an identification of a specified blood processing procedure that is to be performed, displaying on the screen an ...

Methods And Systems For Reducing The Risk Of Bacterial Contamination In Collected Platelets

Methods and systems for reducing bacterial contamination of platelets are disclosed. The methods and systems disclosed herein provide for the processing of a pre-determined volume of whole blood so as to reduce the risk that platelets separated and collected from the whole blood have a reduced risk of bacterial contamination. 1. A method for reducing the risk of bacterial contamination in collected blood platelets comprising:a) inserting a needle of a blood processing kit into a donor to access the vascular system of the patient;b) withdrawing a first pre-determined volume of whole blood from a donor;c) introducing said first pre-determined volume of said whole blood into at least a portion of said blood processing kit;d) returning at least substantially said first pre-determined volume to said donor; ande) withdrawing a second volume of whole blood from said donor.2. The method of further comprising separating and collecting platelets from said second volume of whole blood.3. The method of further comprising collecting a portion of whole blood from said donor in a sample collection container prior to withdrawing said first pre-determined volume of whole blood.4. The method of further comprising introducing a portion of said first pre-determined volume whole blood into a different portion of said blood processing kit.5. The method of comprising introducing said first pre-determined volume of said whole blood into a chamber of a multi-chambered blood processing container comprising first and second sub-chambers in fluid communication with one another.6. The method of comprising removing said first pre-determined volume from said one sub-chamber of said multi-chambered blood processing container without said first pre-determined volume entering said second sub-chamber.7. The method of comprising preventing said first pre-determined volume from entering said second chamber of said multi-chambered blood processing container.8. The method of comprising separating said ...

METHODS AND SYSTEMS FOR THE DETECTION AND REMOVAL OF PATHOGENS FROM BLOOD

The invention relates to methods and systems for removal of pathogens from blood or blood products. The invention further relates to methods and systems for treatment and diagnosis of infection in the blood and/or sepsis in a patient in need thereof. 1. A method for the removal of a pathogen from blood or a blood product comprising red blood cells , said method comprising:(a) separating said blood or blood product into at least two components, a first component comprising red blood cells (RBCs) and substantially free of white blood cells (WBCs) and platelets, and a second component comprising WBCs and platelets and substantially free of RBCs;(b) contacting said first component with a filter comprising a non-specific RBC adhesion molecule binder thereby depleting said first component of RBCs that have bound at least one pathogen; and(c) optionally combining said first component that is depleted of RBCs that have bound at least one pathogen and said second component.2. The method of claim 1 , further comprising storing said first component that is depleted of RBCs that have bound at least one pathogen and said second component.3. A method for the removal of a pathogen from blood of a patient in need thereof comprising:(a) withdrawing blood from said patient;(b) separating said blood into at least two blood components, a first component comprising red blood cells (RBCs) and substantially free of white blood cells (WBCs) and platelets, and a second component comprising WBCs and platelets and substantially free of RBCs;(c) contacting said first component with a filter comprising a non-specific RBC adhesion molecule binder thereby depleting said first component of RBCs that have bound at least one pathogen;(d) optionally combining said first component that is depleted of RBCs that have bound at least one pathogen and said second component; and(e) returning said first component that is depleted of RBCs that have bound at least one pathogen and said second component to said ...

Systems and methods for collection of red blood cells

A method and system for collecting a double volume of red blood cells using a spinning membrane separator is provided by which a first quantity of whole blood is withdrawn from a donor; a first quantity of whole blood is flowed to the spinning membrane separator; where it is separated into a first quantity of red blood cells and a first quantity of plasma. The first quantity of red blood cells and the first quantity of plasma are then flowed to respective collection containers, and at least a portion of the first quantity of plasma is returned to the donor. A second quantity of whole blood withdrawn from the donor, and then separated into a second quantity of red blood cells and a second quantity of plasma using the spinning membrane separator. The second quantity of red blood cells and the second quantity of plasma are then flowed to the respective collection containers, and at least a portion of the second quantity of plasma is returned to the donor.

SURGICAL SWAB WASHING METHOD AND APPARATUS

Automated methods and apparatus for washing surgical swabs to extract viable red blood cells from said swabs. One method comprises contacting at least one swab retaining viable red blood cells with saline-based wash solution in a receptacle under sterile conditions; and effecting the automated compression and/or agitation of the receptacle containing the swab(s), saline-based wash solution and red blood cells to facilitate extraction of viable red blood cells from the swabs into the saline-based wash solution. Another method comprises providing at least one swab retaining viable red blood cells in a receptacle; providing saline-based wash solution in said receptacle so that it contacts said swab(s) such that viable red blood cells are extracted from the swab(s) into the saline-based wash solution; effecting automated compression and/or agitation of the receptacle containing the swab(s), saline-based wash solution and red blood cells to facilitate extraction of viable red blood cells from the swab(s) into the saline-based wash solution; and pumping saline-based wash solution containing viable red blood cells extracted from the swab(s) to a reservoir. 181-. (canceled)82. A method for washing surgical swabs to extract viable red blood cells from said swabs , the method comprising:contacting at least one swab retaining viable red blood cells with a saline-based wash solution in a receptacle under sterile conditions; andeffecting at least one of automated compression and automated agitation of the receptacle containing said at least one swab, saline-based wash solution and red blood cells to facilitate extraction of viable red blood cells from said at least one swab into the saline-based wash solution.83. A method according to claim 82 , wherein said receptacle is flexible.84. A method according to claim 83 , wherein the method comprises automated compression of the receptacle containing said at least one swab claim 83 , saline-based wash solution and red blood cells by ...

Cell Washing Device Using Standing Acoustic Waves And A Phantom Material

Devices for washing a composition including cells are provided. The devices include a separation channel, a first wave component and a second wave component. The separation channel is positioned between the first and second wave components. Collectively, the wave components generate a standing bulk acoustic wave that isolates cells from the composition.

Systems, Methods, and Devices for Removing Circulating Tumor Cells from Blood

A crossflow filter includes a rigid cylindrical inner wall and a rigid cylindrical outer wall inner with an inelastic filter membrane positioned therebetween defining a retentate channel inside the filter membrane and a permeate channel outside the filter membrane. Further, the filter includes transition channels shaped and connected to the inner and outer walls to deliver a flow of fluid from an inlet port to the retentate channel and to capture flow flowing longitudinally along the cylindrical inner and outer walls from both the retentate and permeate channels to respective outlet ports.

Systems And Methods For Leukoreducing A Red Blood Cell-Containing Fluid And Concentrated Red Blood Cells

Systems and methods are provided for separating a red blood cell-containing fluid into separated red blood cells and another fluid constituent. A suitable system includes a disposable fluid flow circuit and a durable, reusable separation system, with the circuit being mounted onto or otherwise associated with the separation system. The circuit includes a membrane separator for separating the fluid into its constituent parts, as well as a leukoreduction filter. The leukoreduction filter may be used before or after the red blood cell-containing fluid has been passed into the membrane separator. The red blood cell-containing fluid (if the leukoreduction filter is positioned upstream of the membrane separator) or the separated red blood cells (if the leukoreduction filter is positioned downstream of the membrane separator) may also be passed through a microaggregate filter prior to passing through the leukoreduction filter. 1. A fluid flow circuit for separating an anticoagulated red blood cell-containing fluid into separated red blood cells and another fluid constituent , the fluid flow circuit comprising:a separator including an inlet port and a red blood cell outlet port and configured to separate an anticoagulated red blood cell-containing fluid into separated red blood cells and another fluid constituent;a fluid source container configured to contain an anticoagulated red blood cell-containing fluid;an inlet flow path in fluid communication with the inlet port and the fluid source container for flowing the anticoagulated red blood cell-containing fluid from the fluid source container to the inlet port; anda leukoreduction filter associated with the inlet flow path.2. The fluid flow circuit of claim 1 , wherein the anticoagulated red blood cell-containing fluid is anticoagulated whole blood and the leukoreduction filter is configured to reduce the amount of leukocytes in the anticoagulated whole blood.3. The fluid flow circuit of claim 1 , further comprising a ...

ACOUSTIC SEPARATION FOR BIOPROCESSING

A method for separating cells in a biofluid includes pretreating the biofluid by introducing an additive, flowing the pretreated biofluid through a microfluidic separation channel, and applying acoustic energy to the microfluidic separation channel. A system for microfluidic cell separation, capable of separating target cells from non-target cells in a biofluid includes at least one microfluidic separation channel, a source of biofluid, a source of additive, and at least one acoustic transducer coupled to the microfluidic separation channel. A kit for microfluidic cell separation includes a microfluidic separation channel connected to an acoustic transducer, a source of an additive, and instructions for use. 1. A method of separating target cells from non-target cells in a biofluid , comprising:pretreating the biofluid by introducing an additive to alter at least one of size of the target cells, size of the non-target cells, compressibility of the biofluid, compressibility of the target cells, compressibility of the non-target cells, aggregation potential of the target cells, and aggregation potential of the non-target cells;flowing the pretreated biofluid into an inlet of a microfluidic separation channel; andapplying acoustic energy to the microfluidic separation channel, such that the target cells accumulate within at least one primary stream along the separation channel and the non-target cells accumulate within at least one secondary stream along the separation channel.2. The method of claim 1 , further comprising collecting the at least one primary stream comprising the target cells.3. The method of claim 2 , further comprising separately collecting the at least one secondary stream comprising the non-target cells.4. The method of claim 1 , further comprising selecting the additive from the group consisting of a cell aggregator claim 1 , deionized water claim 1 , a detergent claim 1 , a surfactant claim 1 , a solution to regulate salinity of the biofluid claim ...

METHODS AND SYSTEMS FOR REDUCING THE RISK OF BACTERIAL CONTAMINATION IN COLLECTED PLATELETS

Methods and systems for reducing bacterial contamination of platelets are disclosed. The methods and systems disclosed herein provide for the processing of a pre-determined volume of whole blood so as to reduce the risk that platelets separated and collected from the whole blood have a reduced risk of bacterial contamination. 1. A method for reducing the risk of bacterial contamination in collected blood platelets comprising:a) inserting a needle of a blood processing kit into a donor to access the vascular system of the patient;b) withdrawing a first pre-determined volume of whole blood from a donor;c) introducing said first pre-determined volume of said whole blood into at least a portion of said blood processing kit;d) returning at least substantially said first pre-determined volume to said donor; ande) withdrawing a second volume of whole blood from said donor.2. The method of further comprising separating and collecting platelets from said second volume of whole blood.3. The method of further comprising collecting a portion of whole blood from said donor in a sample collection container prior to withdrawing said first pre-determined volume of whole blood.4. The method of further comprising introducing a portion of said first pre-determined volume whole blood into a different portion of said blood processing kit.5. The method of comprising introducing said first pre-determined volume of said whole blood into a chamber of a multi-chambered blood processing container comprising first and second sub-chambers in fluid communication with one another.6. The method of comprising removing said first pre-determined volume from said one sub-chamber of said multi-chambered blood processing container without said first pre-determined volume entering said second sub-chamber.7. The method of comprising preventing said first pre-determined volume from entering said second chamber of said multi-chambered blood processing container.8. The method of comprising separating said ...

Systems And Methods For Determining Replacement Fluid And Plasma Flow Rates For Red Blood Cell Exchange Procedures

Systems and methods for performing a red blood cell exchange procedure are disclosed. In one aspect, the flow rates of a replacement fluid (e.g., red blood cells from a healthy donor) and plasma being flowed to a blood source are calculated based on certain parameters of the procedure that are known, including the flow rate of blood being drawn from the source and the volume of replacement fluid to be used during the procedure. The replacement fluid flow rate and the plasma flow rate are calculated in a way that allows for simultaneous depletion of the supply of replacement fluid and achievement of another prescribed process parameter. The other prescribed process parameter depends on the nature of the procedure, namely whether the fluid volume and hematocrit of the source are to change by the end of the procedure or remain the same. 1. A blood processing system comprising:a blood separation device configured to separate whole blood from a blood source into a first component including separated red blood cells and a second component including separated plasma;{'sub': '3', 'an inlet pump operable to convey whole blood from the blood source into the blood separation device at a prescribed rate Q;'}a plasma pump operable to convey separated plasma from the blood separation device to the blood source at a plasma flow rate;a replacement fluid pump operable to convey a replacement fluid to the source at a replacement fluid flow rate; and [{'sub': 3', 'R, 'calculate the plasma flow rate and the replacement fluid flow rate based at least in part on Qand a volume Vof replacement fluid to be flowed to the source,'}, 'adjust the operation of the replacement fluid pump to achieve the calculated replacement fluid flow rate, and', {'sub': 'R', 'adjust the operation of the plasma pump to achieve the calculated plasma flow rate, wherein the plasma flow rate and the replacement fluid flow rate are calculated so as to simultaneously deplete the volume Vof replacement fluid and ...

Centrifugal Syringe And Method For Blood Fractionation

Disclosed is a centrifugable syringe and syringe components for use in blood fractionation. Also disclosed is a method of blood fractionation using the syringe. The syringe comprises a substantially transparent barrel, a substantially transparent, elongated delineation neck, a plunger for drawing blood into and expelling blood from the barrel, and an adapter attached to the neck for enabling releasable connection of a selected device over an inlet/outlet opening in the neck. The delineation neck extends away from a distal end of the barrel. The barrel and the neck have respective axial bores in fluid flow communication with each other, the cross sectional area of the bore in the delineation neck being substantially less than the cross sectional area of the bore in the barrel. The selected device may be one of various devices including a capping device sealing the inlet/outlet opening of the neck, a needle device used during withdrawal of blood from a subject through the neck, or a hose device used to carry away delineated blood fractions from the syringe through the neck.

SELECTIVE REMOVAL OF AGE-MODIFIED CELLS FOR TREATMENT OF ATHEROSCLEROSIS

A method of treating atherosclerosis comprises removing AGE-modified cells from a patient. The AGE-modified cells include erythrocytes, intima cells, endothelial cells, smooth muscle cells, macrophages, and foam cells. A variety of techniques, such as ultrasound and binding with an anti-AGE antibody, may be used to identify and remove the AGE-modified cells.

METHOD OF BLOOD POOLING AND STORAGE

The disclosure provides methods of making a red blood cell, plasma, and platelet products having a uniform dose and volume. The method comprises pooling a plurality of blood units, leukoreducing the blood and inactivating any pathogen contained therein. Plasma, RBCs, and platelets are then divided into uniform dose and volume units which have an extended shelf life. 2. The method of claim 1 , wherein the storage solution comprises at least one material selected from the group consisting of adenine claim 1 , glucose claim 1 , phosphate claim 1 , mannitol claim 1 , guanosine claim 1 , and a combination thereof.3. The method of claim 1 , wherein the treating step inactivates one or more pathogens without damaging the structure or function of a cell component.4. The method of claim 1 , wherein the one or more pathogens are selected from the group consisting of viruses claim 1 , bacteria claim 1 , fungi claim 1 , prions claim 1 , parasites claim 1 , and combinations thereof.5. The method of claim 1 , wherein the one or more pathogens are inactivated by at least one method selected from the group consisting of irradiation claim 1 , solvent and detergent claim 1 , magnetophoresis claim 1 , immunomagnetic bead technology claim 1 , filtration claim 1 , and a combination thereof.6. The method of claim 1 , wherein each unit of the RBC component contains about 1×10to about 5×10RBCs/unit.7. The method of claim 1 , wherein each unit of the RBC component contains about 20-80 g of hemoglobin/unit.8. The method of claim 1 , wherein each unit of the platelet component contains about 2-6×10platelets/unit.9. The method of claim 1 , further comprising the step of filtering out poorly-deformable RBCs from an RBC or blood component.10. The method of claim 1 , wherein the leukoreduction step is performed prior to the pathogen inactivation step.11. The method of claim 1 , wherein the pathogen inactivation step is performed prior to the leukoreduction step.12. The method of claim 1 , wherein ...

BLOOD TREATMENT FILTER AND BLOOD BAG SYSTEM

A blood treatment filter includes a housing having a first resin sheet and a second resin sheet, a filter member disposed in the housing, a peripheral edge molded body formed on a peripheral edge portion of the filter member and having an inner peripheral portion joined to an outer peripheral end edge of a filtering material, and a connection sheet extending outward from the peripheral edge molded body and connected to the housing. 1. A blood treatment filter configured to remove a predetermined blood component from blood , the blood treatment filter comprising:a housing including a first resin sheet and a second resin sheet constituting walls opposite each other;a filter member provided between the first resin sheet and the second resin sheet forming a partition in the interior of the housing into a blood inflow chamber and a blood outflow chamber, the filter member including at least one filtering material;{'b': '54', 'an inflow port in communication with the blood inflow chamber ();'}an outflow port in communication with the blood outflow chamber;a peripheral edge molded body configured to cover a peripheral edge portion of the filter member, the peripheral edge molded body including an inner peripheral edge portion joined to an outer peripheral end edge of the filtering material; anda connection sheet connecting the peripheral edge molded body to the housing.2. The blood treatment filter according to claim 1 ,wherein the connection sheet surrounds the peripheral edge molded body, andthe peripheral edge molded body is connected to the first resin sheet and the second resin sheet via the connection sheet.3. The blood treatment filter according to claim 1 ,wherein the connection sheet includes a first connection sheet provided on the inflow port side, and a second connection sheet provided on the outflow port side,the first resin sheet is connected to the peripheral edge molded body and the first connection sheet, andthe second resin sheet is connected to the ...

BLOOD COMPONENT SEPARATION DEVICE AND BLOOD COMPONENT SEPARATION METHOD

Provided is a blood component separation device and related method, each capable of separating a blood component from blood and rapidly performing a virus inactivation process on the separated blood component. The blood component separation device includes: a blood component separation section, provided in a centrifuge, configured to separate a blood component from blood by centrifugation, a diluting section configured to dilute, in a diluent containing riboflavin, concentrated red blood cells separated by the blood component separation section, and a UV light emitting unit configured to perform a virus inactivation process by exposing the concentrated red blood cells thus diluted to the UV light. 1. A blood component separation device , the device comprising:a blood component separation section configured to separate a blood component from blood by centrifugation;a diluting section configured to dilute, in a diluent containing a photoactive virus inactivation agent, the blood component separated by the blood component separation section; anda blood-component virus inactivation section configured to perform a virus inactivation by exposing the diluted blood component to a UV light source.2. The blood component separation device according to claim 1 , further comprising a concentration adjustment section configured to adjust a concentration of the blood component having been subjected to the virus inactivation process.3. The blood component separation device according to claim 2 , wherein the concentration adjustment section adjusts the concentration of the blood component by centrifuging the blood component.4. The blood component separation device according to claim 3 , wherein the blood component separation section and the concentration adjustment section are provided in a single centrifugal separation device.5. The blood component separation device according to claim 4 , wherein the blood component separation section and the concentration adjustment section are ...

CONNECTIVE TISSUE PROGENITOR CELL ASPIRATION AND PROCESSING SYSTEM

A method of treating soft tissue conditions. A harvesting device is provided. The harvesting device is operably connected to a tissue processing device using tubing. An aperture is formed in a bone. The bone has an interior. The harvesting device is inserted through the aperture in the bone and into the interior of the bone. The harvesting device is manipulated to dissociate connective tissue progenitor cells in the interior of the bone. Tissue is aspirated from the interior of the bone. The connective tissue progenitor cells are separated from the aspirated tissue. The separated connective tissue progenitor cells are injected in a region of a body that is experiencing a soft tissue condition to treat the soft tissue condition. 1. A method of treating soft tissue conditions comprising:providing a harvesting device;operably connecting the harvesting device to a tissue processing device using tubing;forming an aperture in a bone, wherein the bone comprises an interior;inserting the harvesting device through the aperture in the bone and into the interior of the bone;manipulating the harvesting device to dissociate connective tissue progenitor cells in the interior of the bone;aspirating tissue from the interior of the bone;separating the connective tissue progenitor cells from the aspirated tissue; andinjecting the separated connective tissue progenitor cells in a region of a body that is experiencing a soft tissue condition to treat the soft tissue condition.2. The method of claim 1 , wherein manipulating the harvesting device morcelizes bone in the interior to dissociate the connective tissue progenitor cells from the bone and wherein the method further comprises separating bone fragments from the aspirated tissue.3. The method of claim 1 , and further comprising separating red blood cells from the aspirated tissue using a binding agent that is capable of selectively binding with the red blood cells in the aspirated tissue.4. The method of claim 1 , wherein the ...

DEVICES AND METHODS FOR BIO-PROCESSING CELLULAR SAMPLES

The present disclosure relates to completely closed systems suitable for bio-processing of cellular samples, for example peripheral blood samples used for immunotherapy applications, and related methods of use. The systems are not open to the air, thus allowing for sterile sample processing and transfer of the sample throughout the entirety of bio-processing. Each component of the disclosed systems contains a unique identifier, allowing for traceability of the sample as it proceeds through the various steps involved in bio-processing. The identifier ultimately traces the sample back to the patient from which the sample was derived. Certain embodiments provide a unique freezing bag for long-term storage of cellular samples. The freezing bag has a unique identifier that allows for easy traceability and retrieval of a bio-archived sample and at least two ports, one for sample testing, another for sterile docking to a device that allows for delivery of its contents to a patient. 1. A system for cellular bioprocessing and manufacturing , comprising: one or more processing bagsets , a culture bagset and a washing bagset , wherein:each bagset comprises the same 2D barcode that is unique to the system; andeach bagset is configured to be in fluid connection with the other bagsets via one or more luer connections or via one or more sterile docks using a sterile connection device.2. The system of claim 1 , wherein the one or more processing bagsets comprise flexible components.3. The system of or claim 1 , wherein the flexible components comprise a processing bag claim 1 , a red blood cell bag claim 1 , and a cell concentrate bag;wherein the system is closed to the outside environment and the components are in fluid connection with each other via a plurality of tubes.4. The system of claim 3 , wherein:the processing bag is in fluid connection with the red blood cell bag via a first tube;the processing bag is in fluid connection with the cell concentrate bag via a second tube; ...

FAILSAFE SYSTEM AND METHOD FOR A MEDICAL FLUID PROCEDURE

A failsafe system for a medical fluid procedure, comprising a medical fluid processing apparatus comprising a sealer and a programmable controller driven by software, wherein the programmable controller is programmed to recognize a failure event from input from hardware components of the medical fluid processing apparatus. The system also comprises a disposable fluid circuit configured to associate with the medical fluid processing apparatus and comprising a tubing segment configured to fit within the sealer. The programmable controller is configured to seal the tubing segment by activating the sealer surrounding the tubing segment in response to an occurrence of the failure event. 1. A failsafe system for a medical fluid procedure , comprising:a medical fluid processing apparatus comprising a sealer and a programmable controller driven by software, wherein the programmable controller is programmed to recognize a failure event from input from hardware components of the medical fluid processing apparatus;a disposable fluid circuit configured to associate with the medical fluid processing apparatus and comprising a tubing segment configured to fit within the sealer;wherein the programmable controller is configured to seal the tubing segment by activating the sealer surrounding the tubing segment in response to an occurrence of the failure event.2. The failsafe system of claim 1 , wherein the failure event comprises at least one of a leakage in a component of the fluid circuit claim 1 , abnormal hematocrit claim 1 , abnormal fluid volume claim 1 , procedure pause event claim 1 , procedure termination event claim 1 , and failure to detect an authentication mark.3. The failsafe system of claim 1 , wherein the hardware components comprise at least one of the sealer claim 1 , a hematocrit sensor claim 1 , a pressure sensor claim 1 , and scale configured to detect the failure event.4. The failsafe system of claim 1 , wherein the programmable controller is further configured ...

Determination Of Cause Of Redness In Biological Fluid

An optical sensor device includes a light source configured to emit a light including a wavelength in a range of 650 to 900 nm that is exposed to a biological fluid at first and second times. At least a portion of the light is reflected off of the fluid and received by a light detector. The light detector analyzes at least a portion of the received light to determine a first intensity of the light at the wavelength at the first time and a second intensity of the light at the wavelength at the second time. A controller compares the first and second intensities and generates an output indicative of the presence of red blood cells or free hemoglobin in the biological fluid depending on which intensity is greater and whether there is more redness in the biological fluid at the first time or at the second time. 1. A method of determining a cause of redness in a biological fluid , comprising:exposing a biological fluid to a light including a wavelength in a range of 650 nm to 900 nm at a first time and at a second time that is subsequent to the first time so as to cause at least a portion of the light to be reflected by the biological fluid;receiving at least a portion of the reflected light at the first time and at the second time;analyzing at least a portion of the received light at the first time to determine a first intensity of said at least a portion of the received light at said wavelength and at the second time to determine a second intensity of said at least a portion of the received light at said wavelength;determining whether the redness in the biological fluid at the second time is greater or less than the redness in the biological fluid at the first time;comparing the first intensity to the second intensity; andgenerating an output indicative of a presence of red blood cells in the biological fluid or generating an output indicative of a presence of free hemoglobin in the biological fluid, based upon said determination of whether the redness in the biological ...

SYSTEMS AND METHODS FOR DETECTING AN EMPTY WB CONTAINER

A system and method are provided for separating previously-collected whole blood into a red blood cell fraction and a plasma fraction by which the container of previously-collected whole blood is determined to be empty based on using the combination of the measured gross weight of the container and a calculated fluid flow rate from the container, based on weigh scale feedback. Upon detection of the empty container, flow from the container is stopped. 1. A method for separating previously collected whole blood into at least a first red blood cell fraction and a second plasma fraction using a system comprising a disposable fluid flow circuit comprising a separator and a fluid flow path including a whole blood line connected to a container of whole blood and a durable hardware component comprising a weigh scale for the container of whole blood and a pump for acting on the fluid flow path to control the flow of whole blood from the container of whole blood to the separator , the method comprising:determining a target weight for an empty whole blood container;b) determining a target flow ate of whole blood from the container of whole blood indicative of a near empty whole blood container;c) flowing whole blood from the container of whole blood to the separator;d) determining a current weight of the container of whole blood with the weigh scale;e) calculating a flow rate of whole blood from the container of whole blood;f) comparing the current weight of the container of whole blood to the target weight for an empty whole blood container and comparing the calculated flow rate of whole blood from the container of whole blood to the target flow rate of whole blood from the container of whole blood indicative of the near empty whole blood container; andg) stopping the flow of whole blood from the container of whole blood when both the current weight is equal to or less than the target weight and the calculated flow rate is equal to or less than the target flow rate.2. The ...

Process for Preparing Blood Components and Biomedical Device

A process for preparing blood components from blood, by means of a biomedical device (), comprising the steps of: subjecting an isolated blood sample () to a first centrifugation at a speed of 250 rpm for a time of 10 minutes, and to a second centrifugation at a speed of 2000 rpm for a time of 15 minutes. 11621113. A process for preparing blood components from blood , by means of a biomedical device () comprising a first bag () connected to a second bag () and to a third bag () , said process comprising the steps of:{'b': 1', '2', '3', '4, 'a) Subjecting an isolated blood sample (), contained within the first bag (), to a first centrifugation at a speed of 250 rpm for a time of 10 minutes, so as to obtain a sediment consisting of red blood cells () and a supernatant consisting of platelet-rich plasma ();'}{'b': 2', '4', '11, 'b) Transferring from the first bag () said platelet-rich plasma () obtained from step a) to the second bag ();'}{'b': 2', '11', '13', '5', '2', '6', '2', '5', '11', '12', '11, 'c) Subjecting the first bag (), connected to the second bag () and to the third bag (), to a second centrifugation at a speed of 2000 rpm for a time of 15 minutes, so as to obtain a supernatant consisting of platelet-poor plasma () in an upper portion of the first bag () and a red blood cell concentrate () in a lower portion of the first bag (), and a supernatant consisting of platelet-poor plasma () in an upper portion of the second bag () and a platelet pad () in a lower portion of the second bag ();'}{'b': 5', '2', '11', '13, 'd) Transferring the platelet-poor plasma () from the first bag () to the second bag () or to the third bag ();'}{'b': 2', '11', '13', '2', '7, 'e) Separating the first bag () from the second bag () and from the third bag (), and fluidly connecting the first bag () to a storage bag ();'}{'b': 5', '11', '13', '5', '12', '15, 'f) Transferring the platelet-poor plasma () from the second bag () to the third bag (), minus a volume of platelet-poor ...

Blood processing systems and methods with sensors to detect contamination due to presence of cellular components or dilution due to presence of plasma

Blood processing systems and methods employ two sensors, one to detect a condition of plasma exiting a separation device and another sensor to detect a condition of a cellular component exiting the separation device. The first sensor detects, e.g., contamination of the plasma due to presence of unwanted cellular components. The second sensor detects, e.g., dilution of the cellular component due to presence of plasma. Blood processing parameters are carried out based, at least in part, by conditions detected by one or both of the sensors.

Red blood cell processing systems and methods with deliberate under spill of red blood cells

Collection systems and methods use a separation chamber that receives blood or a suspension containing red blood cells and performs a separation process including separation of red blood cells from the blood or suspension. The separation chamber contains, during the separation process, a red blood cell volume. An outlet line is coupled to the separation chamber to remove red blood cells from the chamber device, at least in part, while the separation process occurs. A collection container is coupled to the outlet line to receive a volume of red blood cells removed from the separation chamber. A controller includes a processing function that, during the separation process, includes comparing the volume of red blood cells received by the collection container to a selected targeted red blood cell collection volume to derive a difference. The controller includes another processing function that includes purging substantially all the red blood cell volume occupying the separation chamber into the collection container when the red blood cell volume occupying the separation chamber approximates the difference.

Red blood cell processing systems and methods which control red blood cell hematocrit

Processing systems and methods comprise a separation device that, in use, performs a separation process including separation of red blood cells from blood or a suspension containing red blood cells. The systems and methods include an outlet line coupled to the separation device to remove red blood cells from the separation device, at least in part, while the separation process occurs. A sensor associated with the outlet line senses hematocrit of red blood cells removed from the separation device and generates a sensed hematocrit output. A controller is coupled to the separation device to control removal of red blood cells from the separation device based, at least in part, upon the sensed hematocrit output.

Red blood cell processing systems and methods with deliberate under spill of red blood cells

Collection systems and methods use a separation chamber that receives blood or a suspension containing red blood cells and performs a separation process including separation of red blood cells from the blood or suspension. The separation chamber contains, during the separation process, a red blood cell volume. An outlet line is coupled to the separation chamber to remove red blood cells from the chamber device, at least in part, while the separation process occurs. A collection container is coupled to the outlet line to receive a volume of red blood cells removed from the separation chamber. A controller includes a processing function that, during the separation process, includes comparing the volume of red blood cells received by the collection container to a selected targeted red blood cell collection volume to derive a difference. The controller includes another processing function that includes purging substantially all the red blood cell volume occupying the separation chamber into the collection container when the red blood cell volume occupying the separation chamber approximates the difference.

Red blood cell processing systems and methods which control red blood cell hematocrit

Processing systems and methods comprise a separation device that, in use, performs a separation process including separation of red blood cells from blood or a suspension containing red blood cells. The systems and methods include an outlet line coupled to the separation device to remove red blood cells from the separation device, at least in part, while the separation process occurs. A sensor associated with the outlet line senses hematocrit of red blood cells removed from the separation device and generates a sensed hematocrit output. A controller is coupled to the separation device to control removal of red blood cells from the separation device based, at least in part, upon the sensed hematocrit output.

Red blood cell processing systems and methods which control red blood cell hematocrit

Processing systems and methods comprise a separation device that, in use, performs a separation process including separation of red blood cells from blood or a suspension containing red blood cells. The systems and methods include an outlet line coupled to the separation device to remove red blood cells from the separation device, at least in part, while the separation process occurs. A sensor associated with the outlet line senses hematocrit of red blood cells removed from the separation device and generates a sensed hematocrit output. A controller is coupled to the separation device to control removal of red blood cells from the separation device based, at least in part, upon the sensed hematocrit output.

Red blood cell processing systems and methods which control red blood cell hematocrit

Processing systems and methods comprise a separation device that, in use, performs a separation process including separation of red blood cells from blood or a suspension containing red blood cells. The systems and methods include an outlet line coupled to the separation device to remove red blood cells from the separation device, at least in part, while the separation process occurs. A sensor associated with the outlet line senses hematocrit of red blood cells removed from the separation device and generates a sensed hematocrit output. A controller is coupled to the separation device to control removal of red blood cells from the separation device based, at least in part, upon the sensed hematocrit output.

控制红细胞比容的红细胞处理系统和方法

处理系统和方法，包括在使用中执行分离处理的分离设备，该分离处理包括，从血液或者包含红细胞的悬液中分离红细胞。该系统和方法包括出口管线(104)，其联接到分离设备，用以在分离处理进行时，至少部分地从分离设备移除红细胞。与出口管线(104)相关联的传感器(148)检测从分离设备移除的红细胞的比容，并且产生检测比容输出。控制器联接到分离设备，用以至少部分基于检测比容而控制红细胞从分离设备的移除。

自己輸血装置

(57)【要約】本公報は電子出願前の出願データであるた め要約のデータは記録されません。

处理生物液体的自动化系统和方法

一种用于处理生物液体的自动系统，包括压力差 发生器(51)；生物液体处理装置(10)；和至少偶合于 压力差发生器(51)和生物液体处理装置(10)之一的 自动控制装置(50)。自动系统至少包括在一个壳体 内的一个多孔介质，以便形成过滤器装置；如血红细 胞阻挡过滤器装置(12)，白血球去除过滤器装置(13、 17)和血红细胞阻挡且白血球去除过滤装置。

流体管理系统与方法

宫腔镜流体管理系统包括具有电解质浓度的生理盐水源，至少一个压力机构，其用于使生理盐水循环到目标位置并且从目标位置通过具有过滤器特征的过滤器循环返回到源，以及控制器。控制器以受控流速将在第一流动路径中的生理盐水流入提供到位置以及提供从该位置沿第二流动路径通过过滤器返回到源的生理盐水流出。在存在生理盐水的情况下，在此位置处执行诊断或治疗程序。过滤器特征与受控流速选择为(1)基本上不造成生理盐水中电解质浓度的改变，(2)防止暴露到生理盐水的过滤的红细胞的大于5％的溶血，和/或(3)使血浆暴露到过滤器的凝血酶时间上的效果最小化。

子宮鏡システム

Systems and methods for removing undesired matter from blood cells

Systems and methods of collecting blood cells, substantially free of undesired matter, use a first container (16), that forms a part of blood collection system (10), to initially collect a quantity of blood cells. A filtration system (14) is then attached to the first container. The filtration system (14) includes a second container (34), a first fluid path (36) that leads to the second container (34) through a filtration device (40), and a second fluid path (38) that leads to the second container (34) bypassing the filtration device (40). Blood cells are conveyed from the first container (16) through the first fluid path (36) and filtration device (40) and into the second container (34) to separate the undesired matter from blood cells. Blood cells, now substantially free of the undesired matter, are then conveyed from the second container (34) through the second fluid path (38), bypassing the filtration device (40), and back into the first container (16). The filtration system (14) is then detached from the blood collection system (10).

Blood component separation system, separation material

Systems and methods for removing undesired matter from blood cells

Systems and methods of collecting blood cells, substantially free of undesired matter, use a first container, that forms a part of a blood collection system, to initially collect a quantity of blood cells. A filtration system is then attached to the first container. The filtration system includes a second container, a first fluid path that leads to the second container through a filtration device, and a second fluid path that leads to the second container bypassing the filtration device. The blood cells are conveyed from the first container through the first fluid path and filtration device and into the second container to separate the undesired matter from the blood cells. The blood cells, now substantially free of the undesired matter, are then conveyed from the second container through the second fluid path, bypassing the filtration device, and back into the first container. The filtration system is then detached from the blood collection system.

Female tire blood group incompatibility adsorbing therapy instrument

本发明涉及医学领域中一种母胎血型不合吸附治疗仪，其特征在于分别以渗透浓度为25和35mmol/L的PB作为裂解液，充分洗涤Rh阳性O型红细胞并以生理盐水清洗，制成透析了血红蛋白但保留抗原性能有效吸附Rh抗体的血影细胞，分别以经100℃溶化后保温在56℃的0.9％、1.0％、1.1％、1.2％、1.3％琼脂糖配成95％血影细胞浓度的吸附剂，按琼脂糖浓度从高到低依次取55～65ml加入以高生物相容性材料制成的圆柱形容器内，制成使其中的吸附剂从进样端到出样端形成血影细胞均匀分布而琼脂糖浓度从低到高的吸附器，用以清除滤过吸附器的血浆中的Rh抗体及被破坏红细胞产生的大分子致病物质，从而达到避免孕妇Rh抗体进入胎儿血液和减轻病情的治疗目的。

How to treat red blood cells

Method and system for removing unwanted substances from blood cells

Method for removing tumor cells from stem cell products contaminated with tumor cells

(57)【要約】 腫瘍細胞に汚染された幹細胞産物から腫瘍細胞を除去する方法が開示される。この方法の一つの態様は、好ましくは一つ以上の腫瘍細胞低減フィルターパッドからなる腫瘍細胞低減フィルター手段を含む直列の濾過装置に依存する。この腫瘍細胞低減フィルター手段は、濾過された幹細胞産物において少なくとも３０％の幹細胞の回収を可能としつつ少なくとも１０倍の腫瘍細胞の減少をもたらす。

Device for separating red blood cell from blood

A device for separating a red blood cell in blood is provided to perform a red blood cell extraction process rapidly and conveniently, and to rotate a lever of the device automatically with a minute and an organized method. A device for separating a red blood cell in blood includes an injector(100), a cylinder(110), a piston(120), an arm, a holder, an image camera, and a controller. The injector has a screw thread formed according to a circumference of a nozzle. A minute controller(200) is combined on the injector. The minute controller has a protruded tip to a direction. The screw thread is formed according to an inner circumference of the tip. The cylinder has a hollow part. The piston includes a head, a rod, and a lever. The arm is installed at the bottom of the lever. The arm comprises a nipper, a shaft, and a speed reduction motor.

System for Filtering Medical and Biological Fluids

의료 및 생물성 유체 등의 현탁액을 여과하는 장치 및 방법으로서, 그 일면에서는, 제1형의 입자인 적혈구 입자(104)가 제2형의 입자인 백혈구(108) 입자보다 비교적 낮은 힘으로 변형되고/되거나 빠른 속도로 변형되는, 크기 및 모양이 다른 2형 이상의 입자들을 포함하는 현탁액을 분리하는 것이다. Apparatus and method for filtering suspensions of medical and biological fluids and the like, in one aspect of which red blood cell particles 104, which are particles of type 1, are deformed at a relatively lower force than white blood cells 108 particles, which are particles of type 2, And / or to separate suspensions of two or more types of particles of different sizes and shapes, which deform rapidly. 제1형의 입자(104)는 손상없이 또는 최소한의 손상만으로 통과시키지만 제2형의 입자(108)는 상당한 손상을 일으켜야만 통과시키도록 상당히 정밀하게 설계된 동공(102)들을 가지는 여과막(100)을 제공한다. 여과막(100)은 동공(102)들 사이의 더 작은 간격에도 불구하고 동공(102)들 사이의 간격이 유지되도록 정밀하게 설계된 동공(102)들을 가지기 때문에, 상기 막(100)의 다공성은 비교적 상당히 커서 전단 응력 노출 시간은 감소시키면서 여과 속도는 더 크게 함으로써 결과적으로 입자의 손상을 감소시키게 한다. 또한 막(100)이 막히는 것을 방지하는 여러 방법을 개시한다. Particles of type 1 104 pass through without damage or with minimal damage, while particles of type 2 108 have filtration membranes 100 having pupils 102 that are designed with great precision to pass only after significant damage has occurred. to provide. Since the filtration membrane 100 has pupils 102 precisely designed such that the spacing between the pupils 102 is maintained despite the smaller spacing between the pupils 102, the porosity of the membrane 100 is relatively significant. As a result, the shear stress exposure time is reduced while the filtration rate is increased, resulting in reduced particle damage. Also disclosed are various methods of preventing the membrane 100 from being blocked.

Be used to handle the system and method for biofluid

一个生物流体处理系统包括：一个生物流体的第 一容器，该容器与包含红细胞障壁介质的第一有作用 的生物医学装置和一个第三接收容器相通；一个包括 白细胞脱除介质的第二有作用的生物医学装置，该装 置与第一容器和第二接收容器相通。

Monkey-people's cell merges the preparation of female tire blood group incompatibility treatment hybrid strain

一种用于医学领域的猴‑人细胞融合母胎血型不合治疗杂交株的制备，其特征在于，以促红细胞生成素为诱导剂，培养Rh阳性“O”型恒河猴骨髓细胞，以增加定向发育的红系细胞，继之将培养后的恒河猴骨髓细胞与人骨髓瘤细胞融合为杂交细胞，再以促红细胞生成素诱导杂交细胞定向分化，以HAT筛选杂交细胞克隆，以Rh抗体筛选能在体外无限扩增的Rh阳性细胞杂交株，经产业化扩增后作为吸附剂，制成吸附器，然后与血液分离器共同构成体外吸附装置，通过吸附母胎血型不合孕妇外周血浆中的致病性Rh抗体，创建不清除血浆及其有益成份的无需血浆置换液的廉价安全的母胎血型不合溶血病的治疗新方法。

Method for setting a blood transfusion parameter

One variation of a method for setting a blood transfusion parameter for a patient includes identifying a blood transfusion bag in a photographic image; extracting a color feature from a region of the photographic image corresponding to the blood transfusion bag; estimating a blood component content within the blood transfusion bag based on the color feature; and triggering transfusion from the blood transfusion bag based on the blood component content within the blood transfusion bag and an estimated volemic status of the patient.

Blood component separation system, separation material

Cell washing device using a bulk acoustic wave with phantom material

Separation devices for separating a component from a multicomponent mixture are provided. The separation devices can include a body that defines a separation channel with a cross-sectional geometry that is not circular, rectangular, or square. The separation devices can be positioned relative to a base having an acoustic wave generator. A standing bulk acoustic wave generated by the acoustic wave generator can separate one or more components from the multicomponent mixture. Methods are provided for separating one or more components from a multicomponent mixture using the separation devices.

Cell washing using acoustic waves

Disclosed is a device for separating a cellular component from a multicomponent fluid. The device can include a body, a first acoustic wave generator, and a second acoustic wave propagating component. The body can define a channel having a first surface, an opposing second surface, a first side, and an opposing second side. The channel can extend along a longitudinal axis from a first end to an opposing second end. The first acoustic wave generator can be coupled to the first surface. The second acoustic wave propagating component can be coupled to the second surface. The first acoustic wave generator and second acoustic wave propagating component can be configured to generate a bulk standing acoustic wave in the channel.

Device and method for analysis of blood components and identifying inhibitors and promoters of the inflammatory response

The present invention provides in vitro models of the in vivo rolling and arrest of leukocytes along the endothelial cell wall, which are important steps in the migration of leukocytes out of the blood stream and into tissue, as part of the inflammatory response. The in vitro models of the invention are functional under physiologic flow conditions resulting in physiologic shear stresses. In a specific embodiment, for modelling leukocyte rolling, the apparatus of the invention comprises a solid phase surface with rolling mediator molecules present thereon. Such rolling mediators are, for example, selectins and selectin ligands which have binding partners expressed on leukocytes. In another specific embodiment, for modelling leukocyte rolling followed by adhesion/arrest, the apparatus of the invention comprises a solid phase surface with both rolling mediators and integrin binding partners present thereon. The apparatuses of the invention can be used for collecting, concentrating, purifying, and analyzing blood and blood components, in particular, leukocytes and subsets thereof. The invention further relates to methods for identifying inhibitors or, alternatively, promoters (agonists, functional components) of the inflammatory response. Therapeutic and diagnostic methods, pharmaceutical compositions and kits are also provided.

Blood component separation system and separation material

본 발명은, 각 혈구 성분을 함유하는 체액으로부터, 원심 조작을 필요로 하지 않고, 신속하고 간편하게 적혈구 풍부 분획, 백혈구 풍부 분획, 혈소판 풍부 분획으로 분리하는 시스템 및 분리재에 관한 것이다. 혈구 분리재에 체액을 접촉시켜, 백혈구와 혈소판을 포착시켜 적혈구 풍부 분획을 얻는 공정, 분리 용액을 사용하여 혈구 분리재로 포착된 백혈구 분획을 회수하는 공정을 실시함으로써, 체액으로부터 적혈구 풍부 분획, 백혈구 풍부 분획, 혈소판 풍부 분획으로 분리할 수 있다. The present invention relates to a system and a separating material for rapidly and conveniently separating a body fluid containing each blood cell component into an erythrocyte-rich fraction, a leukocyte-rich fraction and a platelet-rich fraction without centrifuging. A step of bringing a body fluid into contact with a hemocyte separation material to obtain a red blood cell rich fraction by capturing white blood cells and platelets and a step of recovering a white blood cell fraction captured by a hemocyte separation material by using a separation solution to obtain a red blood cell rich fraction, Rich fraction, and platelet rich fraction.

Fluid management system

The invention present concerns a device for conveying fluid comprising: a first source of a first fluid; a second source of a second fluid; a first tube attached to the first source through which the first fluid is adapted to flow; a second tube attached to the second source through which the second fluid is adapted to flow; a connector to which the first and second tubes are coupled; and a device for distributing the first and second fluids to a particular destination, wherein the first and second tubes are coupled to first and second inputs of the device via the connector.

Blood processing systems and methods with sensors to detect contamination

System for treating transition zone material

ABSTRACT Methods and devices for treating transition zone material are disclosed. Transition zone material is processed to form a supernatant layer which includes platelets and a sediment layer which includes red blood cells, and the supernatant layer is separated from the sediment layer by passing the supernatant layer through a porous medium.

Blood processing system and method comprising a sensor for detecting contamination

INTERNALIZING MACHINE.

Methods and assembly of treatment of a biological fluid.

La présente invention concerne des méthodes et un assemblage de traitement d'un fluide biologique. Les méthodes selon l'invention comprennent le passage d'un fluide biologique par un assemblage de filtre d'élimination des leucocytes et la séparation du gaz déplacé par le fluide biologique par passage du gaz par un milieu de barrage de liquide. Les méthodes et l'assemblage du traitement de la présente invention peuvent en particulier trouver application dans le domaine du traitement des fluides biologiques. The present invention relates to methods and assembly for treating a biological fluid. The methods of the invention include passing a biological fluid through a leukocyte removal filter assembly and separating the gas displaced by the biological fluid by passing the gas through a liquid barrier medium. The methods and assembly of the treatment of the present invention may in particular find application in the field of the treatment of biological fluids.