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Небесная энциклопедия

Космические корабли и станции, автоматические КА и методы их проектирования, бортовые комплексы управления, системы и средства жизнеобеспечения, особенности технологии производства ракетно-космических систем

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Мониторинг СМИ

Мониторинг СМИ и социальных сетей. Сканирование интернета, новостных сайтов, специализированных контентных площадок на базе мессенджеров. Гибкие настройки фильтров и первоначальных источников.

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Поддерживает ввод нескольких поисковых фраз (по одной на строку). При поиске обеспечивает поддержку морфологии русского и английского языка
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Применить Всего найдено 8784. Отображено 100.
08-03-2012 дата публикации

Method and installation for regulating the modifier level in chromatography or supercritical extraction with recycling

Номер: US20120055876A1
Автор: Mohamed Shaimi
Принадлежит: PIC Solution SAS

A chromatography or supercritical extraction method is disclosed, in which the eluent comprises a mixture of a fluid and a modifier and in which the fluid is recycled. One exemplary method comprises an operation consisting in determining at least one quantity linked to the level of modifier that is mixed with the recycled fluid and, if necessary, a correction operation in order to limit variations in the level of modifier in the eluent at the inlet of the column or the extractor. The disclosure also relates to a chromatography or extraction installation.

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17-05-2012 дата публикации

Media For Membrane Ion Exchange Chromatography Based On Polymeric Primary Amines, Sorption Device Containing That Media, And Chromatography Scheme And Purification Method Using The Same

Номер: US20120121819A1
Принадлежит: Millipore Corp

Media and devices, such as anion exchangers including such media, wherein the media is a membrane having a surface coated with a polymer such as a polyallylamine. The resulting membrane offers stronger binding of protein impurities and superior removal of host cell proteins from biological samples than conventional ligands based on quaternary ammonium salts, including trimethylammonium ligands. Also described is a chromatography scheme and method for purifying monoclonal antibodies, wherein the anion exchange sorber is placed downstream of an affinity column (e.g., Protein A or Protein G) and optionally one or more polishing devices such as cationic exchange columns. Little or no dilution of the cation exchanger pool (or affinity column exchange pool where no cation exchanger is used) is necessary to lower the conductivity of the sample. The sorber functions well to strongly bind host cell proteins and other impurities in biological samples even at high conductivities and pH.

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21-06-2012 дата публикации

Elimination of Residual Transfer Line Raffinate from Feed to Increase Normal Paraffin Separation Unit Capacity

Номер: US20120152802A1
Принадлежит: UOP LLC

A process to increase the capacity of the adsorbent in a normal paraffin adsorption separation system is presented. A tertiary flush stream is used to improve the capacity of the simulated moving bed system by flushing residual raffinate from the feed transfer line. The flushing removes residual raffinate containing desorbent that competes with the adsorption of normal paraffins from the feedstream. The flush stream is a material that will displace fluid in the column, but will not enter the pores of the adsorbent.

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16-08-2012 дата публикации

Gradient start up system

Номер: US20120205314A1
Автор: Dale A. Davison
Принадлежит: Teledyne Instruments Inc

To provide or maintain pump prime in a liquid chromatographic system when changing solvents or solvent reservoirs or starting up a chromatographic run, first and second solvents are supplied to a mixer through corresponding first and second lines and from the mixer to a chromatographic column. Air in one of the lines is removed by a pump and the line is filled with solvent.

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13-09-2012 дата публикации

Chromatography of polyolefin polymers

Номер: US20120227469A1
Принадлежит: Dow Global Technologies LLC

The invention provides a method for one-dimensional chromatography of a polyolefin polymer, comprising introducing a solution of the polyolefin polymer into a liquid flowing through a liquid chromatography stationary phase, the liquid chromatography stationary phase comprising graphitic carbon, and wherein the polyolefin polymer emerging from the liquid chromatography stationary phase has a retention factor greater than zero, and wherein the solution introduced into the liquid chromatography stationary phase is subjected to a temperature gradient, and/or the solution is subjected to a solvent gradient. The invention also provides a method for multi-dimensional chromatography of a polyolefin polymer, comprising introducing a solution of the polyolefin polymer into a liquid flowing through a first liquid chromatography stationary phase or a field flow fractionation device, and subsequently flowing the solution through a second liquid chromatography stationary phase, the second liquid chromatography stationary phase comprising graphitic carbon, and wherein the polyolefin polymer emerging from the liquid chromatography stationary phase has a retention factor greater than zero. The invention also provides an apparatus for polyolefin polymer chromatography, comprising a liquid chromatography stationary phase, the liquid chromatography stationary phase comprising graphitic carbon and at least one inert filler.

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29-11-2012 дата публикации

Product recovery from adsorption-separation purge fluids

Номер: US20120302812A1
Принадлежит: UOP LLC

Purge fluid from a vessel head in an adsorption process is distributed to recovery processes according to the purity of product contained in the fluid. Extract-rich fluid thus is routed directly to recovery of the extract product. Distribution preferably is determined by internal positioning of feed, desorbent and product streams in the adsorption vessel.

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07-03-2013 дата публикации

Fractionation of de-asphalted oil of vacuum resid using preparative high performance liquid chromatographic separations

Номер: US20130055795A1
Принадлежит: ExxonMobil Research and Engineering Co

Two quantitative separation approaches to fractionate de-asphalted oils (DAOs) into seven classes of compounds (saturates, 1-4 + ring-aromatics, sulfides, and polars). In the first step (named as “SGS”) of present invention, the DAO of a petroleum vacuum resid is separated in to four classes of compounds, namely saturates, aromatics, and sulfides. In this first step of separation, about 3 grams of a DAO can be separated. Whereas in the second step (named as “ARC” separation) of invention, only less than 300 mg of the aromatic fraction obtained in “SGS” (described above) can be further fractionated at very low temperature (about −40 degrees centigrade) into 4 fractions, namely 1-ring, 2-ring, 3-ring, and 4 + -ring aromatics. The present invention protocol is suitable for a wide range of compositionally different DAOs of petroleum vacuum resids.

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11-04-2013 дата публикации

METHOD FOR PERFORMING MAINTENANCE ON A CHROMATOGRAPHY COLUMN

Номер: US20130087491A1
Принадлежит: GE HEALTHCARE BIO-SCIENCES AB

A method for performing maintenance on a chromatography column () comprising the steps of: 11141. A method for performing maintenance on a chromatography column (; ′; ) comprising the steps of:{'b': 7', '7', '63', '5', '53', '3', '43, 'a) detaching a detachable joint (; ′; ) between a bottom plate (; ) of the chromatography column and a stand (; ) on which the chromatography column is provided, when maintenance is required;'}{'b': 15', '15', '59', '1', '1', '41', '5', '53', '3', '43', '15', '15', '59, 'b) attaching an adaptor (; ′; ) provided in the chromatography system to any part of the chromatography column (; ′; ) making it possible to lift the bottom plate (; ) from the stand (; ) with the adaptor (; ′; ); and'}{'b': 15', '15', '59', '5', '53', '5', '53, 'c) raising the adaptor (; ′; ) and thereby also the bottom plate (; ) such that maintenance can be performed under the bottom plate (; ).'}2. The method of claim 1 , further comprising before step a):{'b': 45', '41', '51', '53, 'releasing a column tube () of the chromatography column () from a lid () and the bottom plate () of the chromatography column; and'}{'b': 45', '51', '53, 'rotating the column tube () out from the lid () and the bottom plate (); and step b) further comprising'}{'b': 59', '53, 'attaching the adaptor () to the bottom plate ().'}3. The method of claim 1 , further comprising:{'b': 15', '59', '5', '53, 'd) locking the adaptor (; ) and bottom plate (; ) in the raised position.'}41141343553343776316166115155911411515. A chromatography system comprising a chromatography column (; ′; ) and a stand (; ) onto which the chromatography column is provided claim 1 , characterised in that a bottom plate (; ) of the chromatography column is detachably attached to the stand (; ) by a detachable joint (; ′; ) and in that a locking means (; ′; ) is provided which during maintenance of the chromatography column can lock an adaptor (; ′; ) of the chromatography system to any part of the chromatography ...

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09-05-2013 дата публикации

FLUID MIXING IN A DISPOSABLE FLUID PROCESSING SYSTEM

Номер: US20130112624A1
Принадлежит: GE HEALTHCARE BIO-SCIENCES AB

High accuracy mixing of fluids in a disposable fluid processing system with at least two pumps is provided by a method where a calibration fluid volume is pumped through each pump via a flow meter at at least one calibration pump speed while registering the flow rate using data output from the flow meter, a pump calibration function is calculated from the calibration pump speed and flow rate data and two or more operation fluids are mixed to a predetermined mixture ratio and predetermined flow rate by controlling the pump speed of the respective pumps in accordance with the pump calibration functions. 12223212050525353423243334101130314041. A method for conveying a mixture of at least two operation fluids to a receptacle (;;) in a disposable fluid processing system (;;) comprising at least one flow meter (;;) and at least two pumps ( ,; ,; ,) , each pump connected to at least one source of fluid ( ,; ,; ,) , said method comprising the steps of{'b': 3', '4', '23', '24', '33', '34', '5', '25', '35', '5', '25', '35, 'a) for each pump pumping a calibration fluid volume through said pump (,;,;,) via the flow meter (;;) at at least one calibration pump speed registering the flow rate using data output from the flow meter (;;),'}b) for each pump calculating a pump calibration function from said calibration pump speed and said flow rate and{'b': 3', '4', '23', '24', '33', '34, 'c) mixing two or more operation fluids to a predetermined mixture ratio and predetermined flow rate by controlling the pump speed of the respective pumps (,;,;,) in accordance with said pump calibration functions.'}2. The method of claim 1 , wherein each calibration fluid volume comprises an operation fluid.38283822232342324333482838222323423243334. The method of claim 1 , wherein before step a) a system of valves (;;) is controlled to disconnect said receptacle (;;) from said at least two pumps ( claim 1 ,; claim 1 ,; claim 1 ,) and before step c) said system of valves (;;) is controlled to connect ...

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09-05-2013 дата публикации

Process for separation by selective adsorption on a solid containing a zeolite with a crystalline structure analogous to im-12

Номер: US20130116485A1
Принадлежит: IFP Energies Nouvelles IFPEN

A process for adsorption separation uses a solid IM-12 type adsorbent to separate a molecular species from any feed.

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16-05-2013 дата публикации

Helium Conservation Device for a Gas Chromatograph

Номер: US20130118231A1
Принадлежит: Thermo Finnigan LLC

A device for conserving helium gas in a gas chromatograph system is characterized in that during the majority of an analytical separation, helium is used as the carrier gas while an auxiliary non-helium gas is used to pressurize the inlet and provide for septum purge and split vent flow. Prior an injection period, a coaxial helium flow is established at the column entrance wherein the coaxial helium flow is less than the column flow. Following the injection and sample transfer period, a coaxial helium flow is established wherein the flow is greater than the column flow.

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16-05-2013 дата публикации

POLYMERIC MATERIAL, PRODUCTION AND USE THEREOF

Номер: US20130118980A1
Принадлежит:

The present invention relates to a polymeric material obtainable by reaction of 1. A polymeric material obtainable by reaction of(A) at least one polyimide selected from condensation products of(a) at least one polyisocyanate having on average at least two isocyanate groups per molecule and(b) at least one polycarboxylic acid having at least 3 COOH groups per molecule or anhydride thereof, with(B) at least one diol or triol.2. The polymeric material according to claim 1 , wherein polyimide (A) is selected from those polyimides that have a molecular weight Mof at least 1000 g/mol.3. The polymeric material according to or claim 1 , wherein claim 1 , as polycarboxylic acid (b) claim 1 , a polycarboxylic acid having at least 4 COOH groups per molecule is selected claim 1 , or the respective anhydride.4. The polymeric material according to any one of to claim 1 , wherein polyisocyanate (a) is selected from hexamethylene diisocyanate claim 1 , tetramethylene diisocyanate claim 1 , isophorone diisocyanate claim 1 , 4 claim 1 ,4′-diphenylmethane diisocyanate claim 1 , 2 claim 1 ,4′-diphenylmethane diisocyanate claim 1 , toluoylene diisocyanate and mixtures of at least two of the above-mentioned polyisocyanates (a).5. The polymeric material according to any one of to claim 1 , wherein polyisocyanate (a) is selected from oligomeric hexamethylene diisocyanate claim 1 , oligomeric tetramethylene diisocyanate claim 1 , oligomeric isophorone diisocyanate claim 1 , oligomeric diphenylmethane diisocyanate claim 1 , trimeric toluoylene diisocyanate and mixtures of at least two of the abovementioned polyisocyanates (a).6. The polymeric material according to any one of to claim 1 , wherein diol (B) is selected from diols having a molecular weight Min the range from 250 to 5000 g/mol.7. The polymeric material according to any one of to claim 1 , wherein diol (B) is selected from polyethylene glycols claim 1 , polypropylene glycols claim 1 , polyester diols claim 1 , polycarbonate diols ...

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30-05-2013 дата публикации

MOBILE PHASE SOLVENT DELIVERY MODULE

Номер: US20130134079A1
Принадлежит: WATERS TECHNOLOGIES CORPORATION

Described is a mobile phase solvent delivery module for liquid chromatography and supercritical fluid chromatography systems. The module includes a pump disposed in a fluid path that conducts a mobile phase solvent. The pump provides the mobile phase solvent at an increased pressure. The module also includes a purification cartridge disposed in the fluid path either before or after the pump. The purification cartridge has an inlet to receive the mobile phase solvent and an outlet to provide a purified mobile phase solvent, and includes a packing material for removing an impurity from the mobile phase solvent. In various embodiments, the module also includes one or more additional components to allow various techniques for regeneration or replacement of spent purification cartridges. For example, the module may also include one or more of a temperature control module, a fluidic switch and one or more additional purification cartridges. 1. A solvent delivery module for a chromatography system , comprising:a fluid path having a path inlet to receive a mobile phase solvent;a pump disposed in the fluid path and having a pump inlet configured to receive the mobile phase solvent and a pump outlet to provide the mobile phase solvent at an increased pressure; anda purification cartridge comprising a packing material for removing an impurity from the mobile phase solvent, the purification cartridge disposed in the fluid path and having an inlet to receive the mobile phase solvent and an outlet to provide a purified mobile phase solvent.2. The solvent delivery module of further comprising a solvent reservoir in communication with the path inlet and configured to hold a mobile phase solvent.3. The solvent delivery module of wherein the outlet of the purification cartridge is communication with the pump inlet.4. The solvent delivery module of further comprising a gradient proportioning valve disposed in the fluid path between the path inlet and the purification cartridge claim 3 ...

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30-05-2013 дата публикации

FITTING ELEMENT WITH HYDRAULIC GRIP FORCE ELEMENT

Номер: US20130134082A1
Автор: Dehmer Bernhard
Принадлежит: AGILENT TECHNOLOGIES, INC.

A fitting element (), in particular for an HPLC application (), is configured for providing a fluidic coupling of a tubing () to a fluidic device (). The fitting element () comprises a gripping piece () to exert—upon coupling of the tubing () to the fluidic device ()—a grip force (G) between the fitting element () and the tubing (). The gripping piece () comprises a hydraulic element () configured to transform an axial force (S) into a hydraulic pressure (P) within the hydraulic element (). The hydraulic pressure (P) in the hydraulic element () causes the grip force (G). 110010102103100. A fitting element () , in particular for an HPLC application () , configured for providing a fluidic coupling of a tubing () to a fluidic device () , the fitting element () comprising{'b': 108', '102', '103', '100', '102, 'a gripping piece () configured to exert—upon coupling of the tubing () to the fluidic device ()—a grip force (G) between the fitting element () and the tubing (), and'}{'b': 108', '170', '170, 'wherein the gripping piece () comprises a hydraulic element () configured to transform an axial force (S) into a hydraulic pressure (P) within the hydraulic element (),'}{'b': '170', 'the hydraulic pressure (P) in the hydraulic element () causes the grip force (G).'}2100170. The fitting element () of or any of the above claims claim 1 , wherein the hydraulic element () is an isotropic hydraulic element.3100. The fitting element () of the preceding claim claim 1 , comprising at least one of:the isotropic hydraulic element is configured to transforms an applied force into an isotropic pressure within the hydraulic element;the isotropic hydraulic element is configured to transforms an applied force into an isotropic pressure within the hydraulic element, at least after a given time period or at a given time constant;the isotropic hydraulic element is configured so that pressure at a boundary surface of the hydraulic element is substantially equal;the isotropic hydraulic ...

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30-05-2013 дата публикации

Liquid-Chromatography Conduit Assemblies Having High-Pressure Seals

Номер: US20130134083A1
Принадлежит: WATERS TECHNOLOGIES CORPORATION

A method for making a liquid-chromatography apparatus includes inserting two inner conduits into an intermediate tube, inserting the intermediate tube into an outer tube, forming a proximal seal between the intermediate tube and at least one of the inner conduits, and forming a distal seal between the intermediate tube and at least one of the inner conduits. A liquid-chromatography apparatus includes an outer tube, an intermediate tube disposed in the outer tube, two inner conduits disposed in the intermediate tube, a proximal seal between the intermediate tube and at least one of the inner conduits, and a distal seal between the intermediate tube and at least one of the inner conduits. 111-. (canceled)12. A liquid-chromatography apparatus , comprising:two inner conduits disposed end-to-end, in contact, to define a low dead-volume interface;an intermediate tube, within which the two inner conduits are disposed, wherein the intermediate tube is attached to at least one of the two inner conduits at a distal seal area spaced from the interface;an outer tube, within which the intermediate tube is disposed, wherein the outer tube is deformed at at least two locations proximal to the interface to form a substantially liquid-tight seal areas between the two inner conduits and the intermediate tube;13. The apparatus of claim 12 , wherein the two inner conduits comprise two liner tubes having different inner diameters.14. The apparatus of claim 13 , further comprising particles packed in the liner tube having a larger inner diameter.15. The apparatus of claim 14 , wherein the liner tube having the larger inner diameter defines a trap column or a separation column.16. The apparatus of claim 15 , further comprising a connector directly attached to an exit end of the column.17. The apparatus of claim 12 , further comprising at least one ferrule disposed about the outer tube adjacent to the interface claim 12 , and crimped to define the proximal seal areas.18. The apparatus of ...

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30-05-2013 дата публикации

Flash chromatography cartridge

Номер: US20130134096A1
Принадлежит: Scientific Plastic Products Inc

A low pressure liquid chromatographic cartridge is provided having a tubular polymer container adapted to receive a chromatographic packing material. The container has an outlet port located at a downstream end of the container and container threads formed on an upstream end of the container. A polymer cap having cap threads located on the cap threadingly engage the container threads. An inlet port is located on an upstream end of the cap. A resilient fluid tight seal is interposed between the polymer cap and container suitable for use in low pressure liquid chromatography. A locking tab on a skirt of the cap engages a recess on the container when the seal engages the cap and container to lock the cap in position relative to the container.

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27-06-2013 дата публикации

Chromatography Device

Номер: US20130161244A1
Принадлежит: HITACHI HIGH-TECHNOLOGIES CORPORATION

Provided is a highly safe chromatography device that does not sacrifice user-friendliness during the addition and replacement of a sample holding container. The chromatography device has doors , a door locking mechanism , and a sensor for detecting whether the doors are open or closed. The doors are opened and closed to place a sample holding container and a sample rack in the chromatography device. The door locking mechanism is operated to lock the doors when the sensor indicates that the doors are closed and the analysis of a sample in the sample holding container starts, and is operated to unlock the doors when a return operation and a washing operation terminate after a needle , a syringe , and other mechanical parts operate. Consequently, the sample holding container can be safely added or replaced without having to perform, for instance, a procedure for executing a pause function. 1. A chromatography device having a door , a lock means for locking the door , and a door open/close detection means for detecting whether the door is open or closed , the door being opened and closed in order to place a sample holding container in the chromatography device , the chromatography device comprising:a control section that switches at least between a standby state and an analysis state and controls the locking of the door in accordance with information indicative of a mechanism operation period in the chromatography device during the analysis state and with a door open/close signal supplied from the door open/close detection means.2. The chromatography device according to claim 1 , wherein the analysis state includes at least an injection period for a sample claim 1 , a return period claim 1 , a washing period claim 1 , and a data acquisition period; and wherein the mechanism operation period includes at least the injection period claim 1 , the return period claim 1 , and the washing period.3. The chromatography device according to claim 2 , wherein the control section ...

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27-06-2013 дата публикации

Single-use manifolds for automated, aseptic handling of solutions in bioprocessing applications

Номер: US20130161245A1
Автор: Karl G. Schick
Принадлежит: Parker Hannifin Corp

Presteralized manifolds are provided which are designed for sterile packaging and single-use approaches. Disposable tubing and flexible-wall containers are assembled via aseptic connectors. These manifolds are adapted to interact with other equipment which can be operated by a controller which provides automated and accurate delivery of biotechnology fluid. The manifold also can be used in conjunction with one or more sensors such as pressure and conductivity sensors that interact with the controller or are connected to a separate user interface. An aseptic environment obtains avoiding or reducing cleaning and quality assurance procedures.

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27-06-2013 дата публикации

Chromatography column and maintenance method

Номер: US20130161248A1
Автор: Stefan Kjell Eriksson
Принадлежит: GE Healthcare Bio Sciences AB

A chromatography column and method of maintenance is described which does not require the use of a hoist or crane for disassembly. The method provides improved operator safety by reducing the need for the operator to work below a suspended or supported load within the column. Furthermore, the removal or replacement of column components is facilitated by providing access to the interior of the column and by the provision of a handling device.

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04-07-2013 дата публикации

Purification of Metals

Номер: US20130171046A1
Принадлежит: Mallinckrodt LLC

A solid composition comprises: MnO 2 ; and a compound represented by the general formula (I) wherein: R is a polymer; each Y is independently a hydrogen or a negative charge; Z is either hydrogen or is not present; each n is independently 1, 2, 3, 4, 5 or 6; wherein the MnO 2 is bound to the compound of formula (I) so as to coat the surface thereof. Such a composition may be used for the separation of polyvalent metal species, such as Mo, from one or more accompanying impurities.

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11-07-2013 дата публикации

WETTING OF A PLASTIC BED SUPPORT FOR A CHROMATOGRAPHY COLUMN

Номер: US20130175219A1
Принадлежит: GE HEALTHCARE BIO-SCIENCES AB

A method for wetting a plastic bed support for a chromatography column, comprising the steps of providing a plastic bed support with a pore structure comprising air in said pore structure and soaking the plastic bed support in an aqueous solution comprising 0.1-30 wt % of a non-surfactant wetting agent, resulting in the removal of at least about 60% of the air from said pore structure. 1. A method for wetting a plastic bed support for a chromatography column , said method comprising the steps of:a. providing a plastic bed support with a pore structure comprising air in said pore structure; andb. soaking the plastic bed support in an aqueous solution comprising 0.1-30 wt % of a non-surfactant wetting agent, resulting in the removal of at least about 60% of the air from said pore structure.2. The method of claim 1 , further comprising:c. applying at least 10 kPa overpressure to said plastic bed support when soaked in said aqueous solution and/or vibrating said plastic bed support when soaked in said aqueous solution.3. The method of wherein step b comprises soaking the plastic bed support in an aqueous solution comprising 0.1-17 wt % claim 1 , such as 12-17 wt % claim 1 , of a non-surfactant wetting agent.4. The method of claim 1 , further comprising:d. washing said plastic bed support to remove said non-surfactant wetting agent.5. The method of claim 2 , wherein step c. comprises applying ultrasound to the plastic bed support.6. The method of claim 1 , wherein in step b. said aqueous solution has either no flash point or a flash point of at least about 30° C. claim 1 , such as higher than about 35° C.7. The method of claim 1 , wherein said non-surfactant wetting agent is selected from the group consisting of alkali metal hydroxides and water soluble C3-C6 alcohols claim 1 , glycols and glycol ethers claim 1 , such as water soluble C3-C6 primary alcohols.8. The method of claim 1 , wherein said non-surfactant wetting agent is selected from the group consisting of n- ...

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25-07-2013 дата публикации

SEPARATING COMPONENTS OF MIXED FLUID USING A FUNCTIONALLY GRADED MATERIAL

Номер: US20130186832A1

A system for separating components of a fluid containing at least a first component and a second component includes a device having an inlet for introducing the fluid into the device, a first outlet for directing the first component of the fluid from the device, and a second outlet for directing the second component of the fluid from the device. A material that has a gradient in properties is located in the device between the inlet and the first and second outlets. The material has a first portion with an affinity for the first fluid component and a second portion with an affinity for the second fluid component. The first portion is positioned with relation to the first outlet such that the first component is directed from said device through the first outlet. The second portion is positioned with relation to the second outlet such that the second component is directed from the device through the second outlet. 1. An apparatus for separating components of a fluid containing at least a first component and a second component , comprising:a device;an inlet in said device for introducing the fluid into said device;a first outlet in said device;a second outlet in said device; anda material in said device between said inlet and said first and second outlets that has a gradient in properties, said material having a first portion with an affinity for the first component of the fluid and a second portion with an affinity for the second component of the fluid;wherein said first portion of said material has a location with relation to said first outlet such that the first component of the fluid is directed from said device through said first outlet, andwherein said second portion of said material has a location with relation to said second outlet such that the second component of the fluid is directed from said device through said second outlet.2. The apparatus for separating components of a fluid of claim 1 , wherein said fluid has a direction of flow between said inlet and ...

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01-08-2013 дата публикации

LOW NOISE BACK PRESSURE REGULATOR FOR SUPERCRITICAL FLUID CHROMATOGRAPHY

Номер: US20130192698A1
Принадлежит: AGILENT TECHNOLOGIES, INC.

A drive mechanism for back pressure regulator used in liquid chromatography, supercritical fluid chromatography, or supercritical fluid extraction allows very fine automated control over a very wide range of pressures by combining a linear actuator compressing a spring, pushing a pin. The nozzle assembly of the regulator comprises a flow through chamber containing a diaphragm and a seat, in which the pin pushes the diaphragm against the seat, together with an upstream pressure sensor and electronic feedback control to the motor of the actuator. The BPR of the embodiments exhibits high pressure stability and extremely low pressure noise, even at moderate to high pressures. The exemplary BPR can be use at either constant pressure or to generate pressure programs where the pressure is varied versus time. Further, the nozzle assembly has a field-replaceable head, requiring no mechanical adjustment on replacement. 1a linear actuator comprising a motor applying a force to a spring, wherein said spring applies a second force to a pin;a nozzle assembly comprising a flow-through chamber with a dimpled diaphragm against a seat of a nozzle body;an inlet tube for transferring a mobile phase into the nozzle assembly; andan outlet tube connected to a shaped orifice which receives the dimpled diaphragm when actuated by the force from the pin.. A drive mechanism for a back pressure regulator, comprising: None.The present invention relates to methods and systems for controlling pressure of a mobile phase flowstream within chromatography and extraction systems, such as liquid chromatography, supercritical chromatography, and supercritical extraction systems, with a pressure regulator.Supercritical fluid chromatography (SFC) is a separation technique similar to high performance liquid chromatography (HPLC), except one of the fluids used as a solvent is a highly compressible liquefied gas. Supercritical fluid extraction (SFE) is a related technique but with somewhat lower requirements ...

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08-08-2013 дата публикации

AN APPARATUS FOR CONTINUOUS SEPARATION OF VALINE AND A METHOD FOR CONTINUOUS SEPARATION OF VALINE USING THE SAME

Номер: US20130204040A1
Принадлежит: CJ CHEILJEDANG CORPORATION

The present invention relates to an apparatus for continuous separation of valine from the mixture comprising amino acids such as leucine, isoleucine, etc. and a method for continuous separation of valine by using the same, and the present invention can continuously separate valine from the mixture comprising amino acids such as leucine, isoleucine, etc. in a high purity and yield. 1. An apparatus for continuous separation of valine , which comprising:a Desorbent port (D);a Feed port (F);a Raffinate port (R);an Extract port (E);{'b': 10', '20', '30, 'a number of rotary valves (, , ) each selectively connected to the ports (D, F, R, E); and'}{'b': 40', '50', '60', '10', '20', '30, 'a number of chromatography zones (, , ) each equipped with every a number of rotary valves (, , ),'}{'b': 10', '20', '30, 'wherein the number of rotary valves (, , ) are connected to each other,'}{'b': 10', '20', '30', '10', '10', '10', '20', '20', '20', '30', '30', '30, 'i': a,', 'b,', 'c', 'a,', 'b,', 'c', 'a,', 'b,', 'c, 'wherein the number of rotary valves (, , ) are equipped with the number of connection ports () () (), respectively, and'}{'b': 10', '10', '10', '20', '20', '20', '30', '30', '30', '10', '20', '30', '10', '20', '30, 'i': a,', 'b,', 'c', 'a,', 'b,', 'c', 'a,', 'b,', 'c, 'wherein only any one of the number of connection ports () () () is opened, along with the rotation of the number of rotary valves (, , ), and subsequently any one of the rotary valves (, , ) selectively connected to each of the ports (D, F, R, E) are changed.'}2. The apparatus for continuous separation of valine according to claim 1 , wherein:{'b': 10', '20', '30, 'the number of rotary valves (, , ) rotate after a short period of time, and'}{'b': 10', '20', '30', '10', '20', '30, 'the rotary valves (, , ) connected to the ports (D, F, R, E) are changed, along with the rotation of the number of rotary valves (, , ).'}3. The apparatus for continuous separation of valine according to claim 2 , wherein:{'b': ...

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15-08-2013 дата публикации

Process For Preparing Liquid Mixtures Of Known pH And Salt Concentration

Номер: US20130206666A1
Принадлежит: WATERS TECHNOLOGIES CORPORATION

A method of preparing a liquid mixture for use in a liquid chromatography system is provided. The mixture comprises one or more acids, one or more bases, one or more salts, and one or more solvents, and the method comprises the steps of: i) calculating pH and/or salt concentration at a particular time t from a user-determined gradient function; and ii) based on the values obtained in step (i), calculating percent acid, percent base, percent salt and percent solvent in the liquid mixture at time t. A liquid chromatography system incorporating such method is also provided. 1. A method of preparing a liquid mixture , the mixture comprising one or more acids , one or more bases , one or more salts , and one or more solvents , the method comprising the steps of:i) calculating pH and/or salt concentration at a particular time t from a user-determined gradient function; andii) based on the values obtained in step (i), calculating percent acid, percent base, percent salt and percent solvent in the liquid mixture at time t.2. The method of claim 1 , wherein the gradient function comprises a fraction multiplier that provides predetermined changes in pH and/or salt concentration claim 1 , each being independent of the other claim 1 , over a time period of interest.3. The method according to or claim 1 , wherein the gradient function is based on the following equation:{'br': None, 'i': y=y', 'y', '−y, 'sub': s', 'n', 's, '+()×fraction\u2003\u2003(equation 1)'}{'sub': s', 'n, 'where y is selected from the group consisting of pH and salt concentration, yis the value at time 1 and yis the value at time 2.'}54. The method according to any one of - claims 1 , wherein for a particular pH or salt concentration the % base can be calculated claims 1 , using interpolation claims 1 , from values provided in a pH look-up table.6. The method according to claim 5 , wherein the pH look-up table is empirically derived or is calculated from the pKof the acid.7. The method according to claim 6 , ...

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05-09-2013 дата публикации

Flash chromatography cartridge

Номер: US20130228502A1
Принадлежит: Scientific Plastic Products Inc

A low pressure liquid chromatographic cartridge has a tubular polymer container that receives a chromatographic packing material. The container has an outlet port located at its downstream end and container threads formed on its upstream end. A polymer cap, with an inlet port on an upstream end, screws onto the container threads. A flange depending from the cap mates with the lip of the container to form a fluid tight seal between the polymer cap and container suitable for use in low pressure liquid chromatography.

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05-09-2013 дата публикации

GRADIENT SEPARATION METHOD TRANSFER FOR LIQUID CHROMATOGRAPHY SYSTEMS

Номер: US20130228513A1
Автор: Andrews Richard W.
Принадлежит: WATERS TECHNOLOGIES CORPORATION

Described is a method of transferring a gradient separation method to a liquid chromatography system. A value of a delivered gradient slope for a gradient separation method performed on a first liquid chromatography system is determined based on a value of a programmed gradient slope for the gradient separation method and a predetermined relationship between delivered gradient slope and programmed gradient slope for the first liquid chromatography system. The gradient separation method is performed on a second liquid chromatography system using a delivered gradient slope having a value equal to the value of the delivered gradient slope determined for the first liquid chromatography system 1. A method of transferring a gradient separation method to a liquid chromatography system , the method comprising:determining a value of a delivered gradient slope for a gradient separation method performed on a first liquid chromatography system, the determination being based on a value of a programmed gradient slope for the gradient separation method and a predetermined relationship between delivered gradient slope and programmed gradient slope for the first liquid chromatography system; andperforming the gradient separation method on a second liquid chromatography system using a delivered gradient slope having a value equal to the value of the delivered gradient slope determined for the first liquid chromatography system.2. The method of wherein the programmed gradient slope and the delivered gradient slope for the first liquid chromatography system are different.3. The method of wherein performing the gradient separation method on the second liquid chromatography system comprises programming a gradient slope for the second liquid chromatography system that is predetermined to achieve the delivered gradient slope for the second liquid chromatography system.4. The method of further comprising determining the relationship between delivered gradient slope and programmed gradient ...

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26-09-2013 дата публикации

SYSTEM AND PROCESS FOR BIOPOLYMER CHROMATOGRAPHY

Номер: US20130248451A1
Автор: Hall Martin, Lacki Karol
Принадлежит: GE HEALTHCARE BIO-SCIENCES AB

A chromatography system for separation of a biopolymer is described, comprising at least one feed tank, at least one hold tank, at least one elution buffer tank, at least one eluate tank, at least two packed bed chromatography columns and for each packed bed chromatography column at least one pump and at least one outlet detector both connected to said each packed bed chromatography column, wherein the feed tank, the hold tank(s), the elution buffer tank and the eluate tank are each connected to the packed bed chromatography columns via a system of valves. 11344445678781011781213781478abc. A chromatography system () for separation of a biopolymer , comprising: at least one feed tank () , at least one hold tank (; , , ) , at least one elution buffer tank () , at least one eluate tank () , at least two packed bed chromatography columns ( , ) and for each column ( , ) at least one pump () and at least one outlet detector () both connected to said each column ( , ) , wherein said feed tank , hold tank(s) , elution buffer tank and eluate tank are each connected to said at least two columns via a system of valves () and wherein said hold tank(s) is/are connected to at least one inlet end () of a column ( , ) and at least one outlet end () of a column ( , ).2212. The chromatography system of claim 1 , further comprising at least one control unit () claim 1 , electrically claim 1 , pneumatically or hydraulically connected to said system of valves ().3151617. The chromatography system of claim 1 , further comprising at least one equilibration buffer tank () claim 1 , at least one wash buffer tank () and/or at least one regeneration liquid tank ().4444414781387abc. The chromatography system of claim 1 , wherein said at least one hold tank (; claim 1 , claim 1 , ) is adapted to receive a fluid from an outlet end () of one column ( claim 1 , ) and to convey a fluid to the inlet end () of another column ( claim 1 , ).578. The chromatography system of claim 1 , wherein said ...

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03-10-2013 дата публикации

ION CHROMATOGRAPHY SYSTEMS WITH FLOW-DELAY ELUENT RECYCLE

Номер: US20130256232A1
Принадлежит:

A chromatographic method including chromatographically separating sample ionic species in an eluent stream, detecting the separated sample ionic species, catalytically combining hydrogen and oxygen gases or catalytically decomposing hydrogen peroxide in a catalytic gas elimination chamber, and recycling the effluent stream from the chamber to the chromatography separation column. The residence time between the detector and the chamber is at least about one minute. Also, flowing the recycle sequentially through two detector effluent flow channels of an electrolytic membrane suppressor. Also, applying heat or UV energy between the detector and the chamber. Also, detecting bubbles after the chamber. Also, a Platinum group metal catalyst and ion exchange medium in the chamber. Apparatus for performing the methods. 1. A chromatography apparatus comprising:(a) an electro elution separator including a chromatographic separation medium disposed in a column lumen, and spaced electrodes in electrical communication with the chromatographic separation medium and disposed to pass an electric current through the chromatographic separation medium;(b) a catalytic gas elimination chamber in fluid communication with the electro elution separator, the catalytic gas elimination chamber being downstream of the electro elution separator, the catalytic gas elimination chamber including a catalyst for combining hydrogen and oxygen gases, or for catalytically decomposing hydrogen peroxide, or both, in an effluent stream to form water and reduce the gas content in the eluent stream; and(c) a detector in fluid communication with the electro elution separator and the catalytic gas elimination chamber, the detector being downstream of the electro elution separator.2. The chromatography apparatus of further comprising:(d) a first conduit for the effluent stream providing fluid communication between the electro elution separator and the catalytic gas elimination chamber.3. The chromatography ...

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03-10-2013 дата публикации

Continuous processing methods for biological products

Номер: US20130260419A1
Принадлежит: TARPON BIOSYSTEMS Inc

The present invention is directed to the development of continuous processing technology for the purification of biopharmaceuticals and biological products, such as monoclonal antibodies, protein therapeutics, and vaccines. Methods for continuous processing of a biological product in a feed stream toward formulation of a purified bulk product are described.

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10-10-2013 дата публикации

METHODS AND COMPOSITIONS FOR CHROMATOGRAPHY

Номер: US20130264263A1
Автор: Herman Heath H.
Принадлежит:

The present invention is directed to methods and compositions for separating and isolating target molecules. In particular, the present invention comprises devices, such as CCDs, that contain particles without the need for support structures. Chromatography separation techniques, including but not limited to, ion exchange, size separation, affinity chromatography, ion exclusion, ligand exchange, reversed phase and normal phase partitioning, are used in the CCD. Methods also include low, medium and high pressure liquid chromatography. Such methods can be used for analytical, semi-preparative processes, initial clarification, preparative filtration and process scale applications. 1. An apparatus for isolating a target molecule , comprising:at least one rotating chamber with an inlet port and an outlet port, the chamber containing a plurality of chromatography particles in an interior thereof and rotating about a horizontal axis to create a centrifugal force on the particles; anda pressurized liquid source containing a heterogeneous liquid comprising the target molecule, the first pressurized liquid source in fluid communication with the inlet port and configured to flow the heterogeneous liquid through the inlet port to the interior of the chamber such that a liquid flow force is imparted on the chromatography particles inside the chamber, wherein the liquid flow force substantially opposes the centrifugal force, wherein a gravitational force contributes to a resultant vector summation of all forces acting on the chromatography particles, and wherein the gravitational, liquid flow, and centrifugal forces substantially immobilize the chromatography particles in a fluidized chromatography bed inside the chamber;wherein the outlet port is configured to discharge the heterogeneous liquid less the target molecule.2. The apparatus of claim 1 , wherein the chamber is configured to adjustably rotate to affect either the density or shape of the fluidized chromatography bed ...

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10-10-2013 дата публикации

Methods and compositions for deacidifying fruit juice

Номер: US20130266706A1

The present invention relates to a method for deacidifying fruit juice comprising the steps of providing a resin having a tertiary amine functionality and contacting the fruit juice with said resin to extract the uncharged free organic acids from said juice. Preferably, this is accomplished by using different column processes that are serially connected such that the substance or material exiting one column process is fed to another column process. The term “column process” is defined herein as the chemical or physical process that occurs in the

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17-10-2013 дата публикации

DISPOSABLE HORIZONTAL OR RADIAL FLOW TYPE CHROMATOGRAPHIC COLUMN

Номер: US20130270167A1
Принадлежит:

A liquid chromatography column, utilizing horizontal or radial flow of sample material passing there through, preferably in inward direction, comprising: a housing defining a chamber therein and including at least one removable end section, e.g. screw lid; a first and second longitudinally extending porous fits positioned within said chamber of said housing; a bed or packing of, preferably particulate, chromatographic separation material positioned within said chamber of said housing and intermediate said porous frits, the first of said porous frits being adjacent said housing and an inlet channel, the second of said porous frits being positioned adjacent a core member and an outlet channel; distribution means operatively connected to said inlet channel; collector means operatively connected to said outlet channel, said distribution means and said inlet channel being constructed to direct associated material to be separated in said bed evenly across a longitudinal length of said bed in a substantially horizontal direction. 17-. (canceled)8. A liquid chromatography column , utilizing radial flow of sample material passing there through in inward direction , comprising: a sealed housing defining a chamber therein and including opposite , said housing closing , longitudinal end sections of which at least one is removable and provided by a lid , and a cylindrical housing wall longitudinally extending between and connecting to said opposite end sections and closing the house; a first and a second longitudinally extending , cylindrical porous frit positioned within said chamber of said housing; a doughnut shaped packing of particulate chromatographic separation material contained in a longitudinally sealed doughnut shaped packing space positioned within said chamber of said housing and intermediate said porous frits , the first of said porous frits being adjacent said cylindrical housing wall and a sample material inlet channel of the housing , the second of said porous ...

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07-11-2013 дата публикации

Downstream bioprocessing device

Номер: US20130296538A1
Автор: Sarfaraz K. Niazi
Принадлежит: Therapeutic Proteins International LLC

Large-scale downstream processing of secreted recombinant proteins is provided in a single device, wherein the contents of a plurality of bioreactors are combined simultaneous to their harvesting and purification resulting in significant savings of time and the cost of manufacturing.

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28-11-2013 дата публикации

CAGED BAGS OF POROUS MATERIALS

Номер: US20130313198A1
Принадлежит: NORTHEASTERN UNIVERSITY

Systems and methods employing beds of bagged and caged absorbent and adsorbent materials are disclosed. These inventions are useful in the area of solid phase extraction. 1. A caged porous bag comprising a bed of solid phase material within a porous meshed cloth bag , at least a first part of the bag being locked in a cage , wherein the bed has a minimum width that is defined as the minimum distance through the bed along a straight line that intersects at least one porous meshed side of the bed at a right angle and passes through a point at the center of mass of the bed , said straight line passing sequentially through a porous meshed side of the bag , the bed , and a second porous meshed side of the bag with said sides of the bag contacting the bed where said line passes through , and wherein the bed has a maximum length which is the distance between the two ends of the bed that are farthest apart , and the ratio of said length to the said width is at least 2.2. The caged porous bag of claim 1 , wherein the ratio of said length to said width of the bed is at least 5.3. The caged porous bag of claim 1 , wherein at least 90% of the volume of the meshed cloth bag is occupied by said bed.4. The caged porous bag of claim 1 , wherein at least one part of the meshed cloth bag is locked in contact with the cage by mechanical stress claim 1 , embedding or covalent binding.5. The caged porous bag of claim 1 , wherein the cage is external to the meshed cloth bag.6. The caged porous bag of claim 1 , wherein the cage comprises a container claim 1 , wherein at least part of the cage is in contact with the inside wall of the container.7. The caged porous bag of any of claim 1 , wherein the pores in the porous meshed cloth are selected from the range of about 1 to about 100 microns.8. The caged porous bag of claim 1 , wherein the cage comprises one or more rigid or semi-rigid meshes.9. The caged porous bag of claim 1 , wherein the cage closes an opening of the meshed cloth bag.10. ...

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05-12-2013 дата публикации

High purity chromatographic materials comprising an ionizable modifier

Номер: US20130319086A1
Принадлежит: Waters Technologies Corp

The present invention provides novel chromatographic materials, e.g., for chromatographic separations, processes for its preparation and separations devices containing the chromatographic material; separations devices, chromatographic columns and kits comprising the same; and methods for the preparation thereof. The chromatographic materials of the invention are high purity chromatographic materials comprising a chromatographic surface wherein the chromatographic surface comprises a hydrophobic surface group and one or more ionizable modifier.

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12-12-2013 дата публикации

Method and System for Liquid Chromatograph with Compressibility and Viscosity Monitoring to Identify Fluids

Номер: US20130327692A1
Автор: BRANN John E.
Принадлежит:

A system for providing a solvent or reagent to a liquid chromatography system comprises: a valve comprising a common port and a plurality of other ports, configurable such that the common port may be fluidically coupled to any one of the other ports; a pump fluidically coupled to the common port of the valve; a plug configured to block flow through a first one of said other ports of the valve; a container containing the solvent or reagent, said container fluidically coupled to a second one of said other ports of the valve; a fluid tubing line having a known resistance to fluid flow fluidically coupled to a third one of said other ports of the valve; and a pressure gauge configured to measure fluid pressure within the pump, wherein the solvent or reagent is provided to the liquid chromatography system by a fourth one of the other ports. 1. A system for providing a solvent or reagent to a liquid chromatography system comprising:a valve comprising a common port and a plurality of other ports, configurable such that the common port may be fluidically coupled to any one of the other ports;a pump fluidically coupled to the common port of the valve;a plug configured to block flow through a first one of said other ports of the valve;a container containing the solvent or reagent, said container fluidically coupled to a second one of said other ports of the valve;a fluid tubing line having a known resistance to fluid flow fluidically coupled to a third one of said other ports of the valve; anda pressure gauge configured to measure fluid pressure within the pump,wherein the solvent or reagent is provided to the liquid chromatography system by a fourth one of the other ports.2. A system as recited in claim 1 , further comprising a second container containing a second solvent or reagent fluidically coupled to a fifth one of said other ports of the valve claim 1 , said system configurable so as to also provide the second solvent or reagent to the liquid chromatography system.3. A ...

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26-12-2013 дата публикации

Pressure Monitor Optimizaiton Of Fluid Path Utilization

Номер: US20130340503A1
Принадлежит: Waters Technologies Corp

A device comprising a pressure monitor and a control means that receives a signal representing measured pressure at the pressure monitor and controls the controllable elements of a fluid system is utilized to monitor a fluid system for error conditions, to optimize operations and to diagnose the fluid system. By following a testing protocol that selectively enables parts of the system, the control means narrows the list of possible failing components. Comparing the measured pressure against normal pressures allows the device to identify error conditions. Determining the volume of fluid being transported and controlling the duration of the flow optimizes operation of the fluid system.

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09-01-2014 дата публикации

SEPARATION PROCESS BY MEANS OF HIGH FLOW CONTINUOUS CHROMATOGRAPHY AND CORRESPONDING DEVICE

Номер: US20140008300A1
Автор: Fauquet Nicolas
Принадлежит:

A method and device are provided for separating fractions of a mixture that is to be separated by liquid phase chromatography. The method includes the steps of: multiple injections of the mixture to be separated, where the injections are made successively into a chromatography column after time intervals A; multiple decanting operations, wherein the fractions of said column are decanted successively after time intervals A, generating an eluate enriched with the fraction of interest, which is created from at least one of said decanted fractions; multiple injections of the eluate collected in the preceding step, wherein the injections of the eluate are carried out successively after time intervals B into a second chromatography column; and multiple decanting operations, wherein the fractions from said second column are decanted successively after time intervals B. 1. A method for separating fractions of a mixture to be separated by liquid chromatography , comprising:multiple injections of the mixture to be separated, wherein the injections are made successively into a chromatography column after time intervals A,multiple decanting operations, wherein the fractions of said column are decanted successively after time intervals A, generating an eluate enriched with the fraction of interest, which is created from at least one of said decanted fractions,multiple injections of the eluate generated in the decanting operations, wherein the injections of the eluate are carried out successively after time intervals B into a second chromatography column, andmultiple decanting operations, wherein the fractions from said second column are decanted successively after time intervals B.2. The method for separating according to claim 1 , wherein the injections of the mixture into a column may be made before first elutions of the fractions of interest are made from the same column.3. The method for separating according to claim 1 , wherein the injections of the mixture into a column ...

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23-01-2014 дата публикации

Separation System

Номер: US20140021117A1
Принадлежит:

A process for separating a product from a multicomponent feedstream to an adsorption apparatus or system is described. The apparatus or system may comprise a moving-bed or a simulated moving-bed adsorption means. The product comprises at least one organic compound, such as an aryl compound with alkyl substitutes. In embodiments the conduits used to supply the feedstream to the apparatus or system are flushed with media of multiple grades. The improvement is more efficient use of the desorbent. In embodiments the process achieves improvements in one or more of efficiency of adsorption separation, capacity of adsorption apparatus systems, and purity of product attainable by adsorption process. 114-. (canceled)15201204205. A simulated moving bed adsorption apparatus , the improvement characterized by a fluid connection between the flush out conduit and the secondary flush input and/or , whereby primary flush out may be routed directly for use as secondary flush in , without being routed through intervening distillation apparatus. This application claims the benefit of U.S. Provisional Application No. 61/319,080, filed Mar. 30, 2010 and U.S. application Ser. No. 12/774,319, filed May 5, 2010, the disclosures of which are incorporated herein by reference in their entireties.The invention relates to a process for separating one or more of the components from two or more multicomponent fluid mixtures, and more particularly to a process for separating organic compounds from such a fluid mixture by means of adsorption apparatus, such as moving-bed or simulated moving-bed adsorption apparatus, and a system comprising such apparatus.Various means are currently available to separate the components of a multicomponent fluid mixture. If the densities of the components differ sufficiently, the effects of gravity over time may be adequate to separate the components. Depending on the quantities of the components involved, a centrifuge may be used to more rapidly separate components ...

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13-02-2014 дата публикации

CHANNEL UNIT FOR LIQUID CHROMATOGRAPH

Номер: US20140042066A1
Принадлежит:

A column container is formed in a bonding portion between a first support plate and a second support plate, and a column is held in the column container. An inlet channel connects the column container to a liquid inlet port. The inlet channel includes a first channel having a small diameter and a second channel having an increasing diameter. An inner surface of the second channel is a hemispherical surface. The radius of the column container is substantially the same as the radius of the hemispherical surface. The distance from an inflow end of the column to the boundary between the first channel and the second channel is substantially the same as the radius of the hemispherical surface. 1. A channel unit comprising:a column including a stationary phase for a liquid chromatograph; anda supporter holding the column,the supporter having a column container, a liquid inlet port, a liquid outlet port, an inlet channel, and an outlet channel are formed in, the column container holding the column, the inlet channel connecting the liquid inlet port to an inflow end of the column, the outlet channel connecting an outflow end of the column to the liquid outlet port,wherein the inlet channel includes a first channel and a second channel, the first channel having a uniform cross-sectional area, the second channel having a cross-sectional area that gradually increases from a boundary between the first channel and the second channel toward the inflow end of the column, andwherein a cross section of the column container, the cross section being perpendicular to an axis of the column container, is circular, an inner surface of the second channel is convex, and a distance from the boundary to the inflow end is substantially the same as a radius of the cross section of the column container.2. The channel unit according to claim 1 , wherein a shape of a cross section of the inner surface of the second channel claim 1 , the cross section being taken along a plane including the axis of ...

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13-03-2014 дата публикации

SIMPLIFIED FILTER

Номер: US20140069871A1
Автор: Brown Dennis B.
Принадлежит: Aquamira Technologies, Inc.

A filter includes a structurally rigid adsorptive filtering medium formed as a sintered porous material chosen to remove targeted contaminant(s). The adsorptive filtering medium forms a hollow chamber sized to receive a mechanical filtering medium which is sized to press outward against the adsorptive filtering medium which supports the mechanical filtering medium thus eliminating a cage and a core to support the mechanical filtering medium and leading to a simplified construction. The fluid enters the filter through substantially the entire face of the adsorptive filtering structure and proceeds through the mechanical filtering medium which is a pleated paper filter. The hollow chamber may be capped at each end such that the caps effectively seal the filter and direct the fluid through the adsorptive filter medium and the mechanical filter medium 1. A filter for filtering contaminants in a fluid from a fluid source , said filter comprising:an adsorptive filter medium (AFM) having an AFM length, an AFM first end, an AFM second end, a wall having an exterior surface positioned to receive said fluid from said fluid source and an interior surface opposite said exterior surface, said wall being formed of a material which has a first porosity to pass said fluid therethrough while inhibiting the passage of first selected contaminants therethrough, said wall being formed of material selected to function as the exoskeleton of said filter, and said wall being formed with said interior surface oriented to define a chamber, and said wall being formed with said interior surface oriented to define a chamber,a mechanical filtering medium (MFM) having a MFM length substantially the same as the AFM length, said MFM being positioned in said chamber to receive said fluid from said AFM, and said MFM being formed of a non rigid material which has a second porosity that is different from said first porosity for allowing said fluid to flow therethrough while inhibiting the passage of ...

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20-03-2014 дата публикации

SYSTEM AND METHOD FOR THE AUTOMATION OF COLUMN AND MEDIA PACKING

Номер: US20140076459A1
Принадлежит: GE HEALTHCARE BIO-SCIENCES CORP.

This invention provides for the fully automated, hands free, packing of chromatography columns by means of delivering a pre-calculated volume of slurry and using two different packing modalities to stop the packing when either 1) this volume has been delivered in the column, or 2) when the adapter is moved to reach a bed height corresponding to the pre-calculated volume. Thus, a chromatography column can be packed in a fully automated fashion and such a column is 1)stable and 2) has the desired performance characteristic. 13-. (canceled)4. A method for automating column packing comprising:determining a slurry concentration of a chromatography medium;determining a column volume in at least one chromatography column;determining a compression factor in the at least one chromatography column for the chromatography medium;calculating a volume of a slurry based on the determined column volume, the determined compression factor and the determined slurry concentration;configuring the calculated volume of the slurry in a slurry tank;positioning a movable adapter of the at least one chromatography column at a low position;positioning or opening a media valve that allows for delivery of the calculated volume of the slurry into the at least one chromatography column;consolidating the chromatography medium;automatically lowering the movable adapter in order to compress the chromatography medium to the determined compression factor; andautomatically terminating the packing of the at least one chromatography column when an optimal column bed height is reached.5. The method of claim 4 , wherein the optimal column bed height occurs when the movable adapter has displaced a volume of liquid equal to a difference in volume between an initial height of the adapter and the final height of the movable adapter.6. The method of claim 4 , wherein the media valve is from the group consisting of nozzle claim 4 , slurry port and slurry valve. This application claims priority to U.S. provisional ...

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20-03-2014 дата публикации

CLASS OF HDAC INHIBITORS EXPANDS THE RENAL PROGENITOR CELLS POPULATION AND IMPROVES THE RATE OF RECOVERY FROM ACUTE KIDNEY INJURY

Номер: US20140081054A1

The invention concerns separation methods and systems including those comprising a continuous chromatographic simulated moving bed integrated with vapor compression distillation to create a high efficiency separations platform applicable to a broad range of separation functions. 1. A process for separation and purification of compounds in a liquid mixture with low energy input , the process comprising:passing a feed mixture comprising a first compound and a second compound into a simulated moving bed chromatography apparatus;the first compound acting as a solvent for the second compound which forms a precipitate at concentrations above a solubility limit;passing an eluent solvent into the simulated moving bed chromatography apparatus to separate the feed into a first stream and a second stream, wherein the first stream has an elevated concentration of the first compound in eluent and the second stream has an elevated concentration of the second compound in eluent, as compared to the feed;passing the first stream to a vapor compression distillation unit to generate a high purity stream of the first compound;vaporizing at least a portion of the eluent from the first stream at a first temperature to form a vapor, compressing the vapor to form an eluent condensate at a second temperature, such that the second temperature is greater than the first temperature, and the eluent condensate has a thermal energy content; andtransferring at least a portion of the thermal energy content of the eluent condensate into the first stream to be used in vaporizing the eluent in the first stream.2. The process of claim 1 , wherein less thermal energy is added to the process than would be needed to vaporize the high purity stream of the first compound.3. The process of further comprising:passing the second stream to a vapor compression distillation unit to recover additional eluent and to generate a concentrated second stream with less eluent.4. A process for separation and purification ...

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03-04-2014 дата публикации

Countercurrent Chromatography Rotor

Номер: US20140091036A1
Автор: Finn Thomas, Knight Martha
Принадлежит: CC BIOTECH LLC

A method for constructing a spiral tube support apparatus used in countercurrent chromatography, improvements to countercurrent chromatography tube support design, and methods of using the improved countercurrent chromatography apparatus are described. The spiral tube support apparatus may be constructed by a shape forming process such as a three dimensional printing process that in turn uses a laser sintering technique, and can be made out of any easily formed material. Shape changes on both the tube support and the top improve the performance of the tube support and ease of manufacturing. The improved tube support and new method of creation permit use in both micro or macro scale preparations and use in small or large molecule preparations. In particular, specific solvent systems are described that permit purification of proteins. 1. A method for manufacturing a countercurrent chromatography rotor comprising forming a first surface which comprises a plurality of interweaved spiral channels; one or more tubing access ports , and one or more curved radial channels , to obtain said rotor.2. The method of claim 1 , wherein said first surface is formed from one or more materials selected from the group consisting of a nylon polymer claim 1 , a plastic claim 1 , a polytetrafluoroethylene claim 1 , a polyvinyl chloride claim 1 , a polystyrene claim 1 , a polyamide PA-220 claim 1 , a photopolymer claim 1 , a FullCure® material claim 1 , a Polyjet 3D Printer® material claim 1 , a monomeric powder claim 1 , and a particulate comprising a metal or a metal composite.3. The method of claim 1 , wherein said forming is a process selected from the group consisting of laser sintering claim 1 , ultraviolet irradiation claim 1 , molding claim 1 , prototyping claim 1 , drilling and machining.4. The method of claim 1 , wherein said first surface further comprises a space in which weights may be placed claim 1 , a loop to hold tubing claim 1 , a retainer support claim 1 , a snap lock ...

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05-01-2017 дата публикации

PROCESS FOR EXTRACTION AND DEBITTERIZING SWEET COMPOUNDS FROM STEVIA PLANTS

Номер: US20170000175A1
Принадлежит:

A method of extracting sweet compounds from plant powder includes mixing powder and deionized water to create a powder slurry, filtering the slurry and adding it to an extraction column, adding an ethanol solution to create an elute, mixing the elute with activated charcoal and filtering the elute, removing the ethanol and water from the elute, and spraying the elute to produce the sweet compounds. 1stevia{'i': stevia', 'stevia, 'i) mixing powder and deionized water at a temperature of between about 45° C. and about 75° C. to create a powder slurry;'}{'i': 'stevia', 'ii) filtering the powder slurry;'}{'i': stevia', 'stevia, 'iii) adding the filtered powder slurry to an extraction column charged with adsorbent polymer resins to provide a powder material:water ratio in the range of between about 1:10 and 1:16 v/v;'}iv) adding an ethanol solution having between about 70% and about 100% ethanol concentration to the extraction column, forming a mixture ratio of liquid slurry:ethanol in the range of between about 1:2.3 and about 1:3.75 v/v to discharge an elute;v) mixing the elute with activated charcoal;vi) filtering the elute;vii) removing the ethanol from the elute;viii) passing the alcohol-free elute through a water extraction device; andix) spraying the elute using a spray drying unit to produce a composition comprising the extracted sweet compounds.. A method for extracting sweet compounds from plant powder, comprising: This application is a Continuation of U.S. patent application Ser. No. 14/678,583, filed Apr. 3, 2015 (now U.S. Pat. No. 9,445,617), which claims priority to and the benefit of U.S. Provisional Application No. 62/026,952, filed Jul. 21, 2014. The entire disclosures of U.S. patent application Ser. No. 14/678,583 and U.S. Provisional Patent Application No. 62/026,952 are incorporated herein by reference.The present application relates generally to the extraction of sweet compounds from and debitterizing the same.Extraction of components of value from ...

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01-01-2015 дата публикации

Fluidic Chip with Displacable Patterned Layer for Detecting Fluid Pressure

Номер: US20150000416A1
Принадлежит: AGILENT TECHNOLOGIES, INC.

A fluidic chip device configured for processing a fluid, wherein the fluidic chip device comprises a plurality of layers laminated to one another, wherein at least a part of the layers comprises a patterned section of an alternating sequence of bars and fluidic channels for conducting the fluid under pressure, the patterned section being configured for being displaceable in response to the pressure, and a pressure detector responding to the displacement of the patterned section by generating a detector signal being indicative of a value of the pressure. 1. A fluidic chip device configured for processing a fluid , the fluidic chip device comprising:a plurality of layers laminated to one another;wherein at least a part of the layers comprises a patterned section of an alternating sequence of bars and fluidic channels for conducting the fluid under pressure, the patterned section being configured for being displaceable in response to the pressure; anda pressure detector configured for responding to the displacement of the patterned section by generating a detector signal being indicative of a value of the pressure.2. The fluidic chip device according to claim 1 , comprising:a further patterned section of an alternating sequence of further bars and further fluidic channels for conducting a further fluid under pressure, wherein the further patterned section is configured for being displaceable in response to the pressure of the further fluid;wherein the pressure detector is configured for responding to the displacement of the further patterned section by generating a further detector signal being indicative of a value of the pressure of the further fluid; anda differential pressure determining unit configured for determining information related to a pressure difference between the fluid and the further fluid based on the detector signal and based on the further detector signal.3. The fluidic chip device according to claim 2 , wherein the further patterned section is ...

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07-01-2016 дата публикации

Mobile Phase Degassing for Nano-Flow Liquid Chromatography

Номер: US20160001198A1

Systems and methods for degassing liquids in nano-flow liquid applications (). In a chromatography embodiment, a system includes a buffer container, a degasser, a buffer pump, a nano-flow pump, and a separation column. The buffer pump is configured to move a buffer from the buffer container through the degasser and the nano-flow pump is configured to move the buffer from the buffer container or the degasser to the separation column. 1. A nano-flow liquid chromatography system , comprising:a buffer container,a degasser in fluid communication with the buffer container,a buffer pump configured to move a buffer from the buffer container through the degasser,a nano-flow pump in fluid communication with at least one of the buffer container and the degasser; anda separation column in fluid communication with the nano-flow pump, wherein the nano-flow pump is configured to move the buffer from one of the buffer container and the degasser to the separation column.2. The nano-flow liquid chromatography system of claim 1 , wherein the buffer pump is configured to move the buffer from the buffer container claim 1 , through the degasser claim 1 , and back into the buffer container.3. The nano-flow liquid chromatography system of and further comprising a tee connection positioned at an outlet of the degasser claim 1 , wherein the tee connection is configured to direct a first portion of the buffer from the degasser toward the buffer container and a second portion of the buffer from the degasser toward the nano-flow pump.4. The nano-flow liquid chromatography system of and further comprising a tee connection positioned at an outlet of the degasser claim 1 , and a waste container in fluid communication with the degasser claim 1 , wherein the tee connection is configured to direct a first portion of the buffer from the degasser toward the waste container and a second portion of the buffer from the degasser toward the nano-flow pump.5. A nano-flow liquid degassing system claim 1 , ...

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01-01-2015 дата публикации

Method for conducting maintenance on a chromatography column

Номер: US20150001775A1
Принадлежит: GE Healthcare Bio Sciences AB

The present invention relates to methods for conducting maintenance on chromatography columns used in industrial-scale chromatography. In particular, the invention is concerned with safer methods for performing maintenance on such columns, such as cleaning and replacing bed supports, distributors, nozzles, O-rings and other column components, by the use of a handling device to support, lift, carry and manipulate such column components.

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04-01-2018 дата публикации

Novel multimodal oscillatory chromatographic purification system

Номер: US20180001227A1
Автор: Michael Dale Hilgert
Принадлежит: Individual

The present invention comprises a novel multimodal chromatography sequence of short length alternating adsorption and size exclusion media operating with gradient elution. The novel multimodal chromatography in an oscillating series utilizes the alternating solvent exchange media to reposition the active region of separation back in phase with the target solutes. Each solvent exchange column bed length in the sequence is designed to achieve a subtle decrease or increase in the solvent gradient (or salt gradient) concentration associated with the two solutes of interest which results in an extension of the active separation or increasing differences in solute velocity for two solutes of interest. The novel oscillatory chronographic system demonstrates much improved separation capability as shown by a one dimensional model.

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02-01-2020 дата публикации

A Method in Continuous Chromatography

Номер: US20200001203A1
Принадлежит:

The present invention relates to a method for purifying a target product in a flow-through chromatography system comprises at least a first column loaded with feed material from a feed source. The at least first column is purged after binding of impurities and wherein the outlet of purged material from the column is subsequently passed to the feed source. 1. A method for purifying a target product in a flow-through chromatography system comprising at least a first column loaded with feed material from a feed source , wherein the at least first column is purged after binding of impurities to produce purged material and wherein the purged material from the column is passed upstream of the at least one column to be re-purified.2. The method according to claim 1 , wherein said upstream passing of the purged material is passing to the feed source.3. The method according to further including the steps of:providing an outlet sensor arranged downstream of at least the first column;loading the first column with feed material from the feed source,detecting impurity breakthrough based on a signal detected by the outlet sensor of the first column,when a predetermined impurity breakthrough is detected, disconnecting the first column from the feed source,purging partly purified feed material from the first column using a purging buffer, andpassing the purged partly purified feed material to the feed source.4. The method according to claim 3 , wherein the or each outlet sensor is a UV sensor and the predetermined impurity breakthrough corresponds to a predetermined percentage of impurities in a target product downstream of the or each column.5. The method according to claim 4 , wherein the predetermined percentage of impurities in the target product is about 1% claim 4 , 10% claim 4 , 20% claim 4 , 50% or 70%.6. The method according to or claim 4 , wherein a UV sensor is provided upstream each column claim 4 , and the method further comprises dynamically controlling the impurity ...

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04-01-2018 дата публикации

Process for the Recovering of Paraxylene

Номер: US20180002253A1
Принадлежит:

Disclosed herein are processes for recovering paraxylene in which a first simulated moving bed adsorption unit is used to produce a paraxylene-rich extract stream that also contains a significant amount of the ethylbenzene and a paraxylene-depleted raffinate stream. Because a significant amount of the ethylbenzene is removed in the paraxylene-rich extract stream (at least enough to limit buildup in the isomerization loop), the paraxylene-depleted raffinate stream may be isomerized in the liquid phase. Avoiding vapor phase isomerization saves energy and capital, as liquid phase isomerization requires less energy and capital than the vapor phase isomerization process due to the requirement of vaporizing the paraxylene-depleted stream and the use of hydrogen, which requires an energy- and capital-intensive hydrogen recycle loop. 1. A process for recovering paraxylene , the process comprising:(a) introducing a hydrocarbon feed stream into a first simulated moving bed adsorption unit, wherein the hydrocarbon feed stream comprises a mixture of paraxylene (PX), metaxylene (MX), orthoxylene (OX), and ethylbenzene (EB);(b) introducing a desorbent stream into the first simulated moving bed adsorption unit, wherein the desorbent stream comprises desorbent;(c) withdrawing a first PX-rich extract stream from the first simulated moving bed adsorption unit, wherein the first PX-rich extract stream comprises desorbent, PX, and EB;(d) withdrawing a PX-depleted raffinate stream from the first simulated moving bed adsorption unit, wherein the PX-depleted raffinate stream comprises desorbent, MX, OX, and EB;(e) isomerizing at least a portion of the first PX-depleted raffinate stream at least partially in the liquid phase to produce an isomerized stream having a higher PX concentration than the first PX-depleted raffinate stream;(f) recycling at least a portion of the first isomerized stream to the first simulated moving bed adsorption unit; and(g) introducing the first PX-rich extract ...

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02-01-2020 дата публикации

VITAMIN E PRODUCTION METHOD AND VITAMIN E PRODUCTION DEVICE

Номер: US20200002305A1
Принадлежит: TOHOKU UNIVERSITY

A vitamin E production method and a vitamin E production device which can highly purify vitamin E in a vitamin E concentrated fraction are provided. A raw oil supply section supplies a raw oil to a series column in which two or more columns including a strongly basic anion exchanger are coupled in series to adsorb vitamin E included in the raw oil on the strongly basic anion exchanger of at least one column from among the series column. A desorption solution supply section supplies a desorption solution to a column on which vitamin E has been adsorbed to desorb vitamin E from the strongly basic anion exchanger of the column. 1. A vitamin E production method for recovering vitamin E included in a raw oil , the method having:an adsorption step of supplying the raw oil to a series column in which two or more columns comprising a strongly basic anion exchanger are coupled in series, thereby adsorbing vitamin E included in the raw oil on the strongly basic anion exchanger of at least one column from among the series column, anda desorption step of supplying a desorption solution to a column on which vitamin E has been adsorbed in the adsorption step, thereby desorbing the vitamin E from the strongly basic anion exchanger of the column.2. The vitamin E production method according to claim 1 , wherein in the desorption step claim 1 , the vitamin E is desorbed from the strongly basic anion exchanger of at least one column from among the series column claim 1 , except for a column through which the raw oil flows first.3. The vitamin E production method according to claim 1 , wherein in the desorption step claim 1 , the vitamin E is desorbed from the strongly basic anion exchanger of claim 1 , from among the series column claim 1 , at least a column through which the raw oil flows last.4. The vitamin E production method according to claim 1 , wherein in the series column claim 1 , the lengths of columns along a direction through which the raw oil flows are the same claim 1 , ...

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02-01-2020 дата публикации

SYSTEMS AND METHODS FOR PREPARING A POLYPEPTIDE FROM A MIXTURE

Номер: US20200002373A1
Принадлежит: Regeneron Pharmaceuticals, Inc.

Embodiments of the present disclosure are directed to methods for preparing a target polypeptide from a mixture including the target polypeptide. The method may include contacting the mixture to a hydrophobic interaction chromatography (HIC) apparatus including multiple chromatographic zones. The method may further include passing the target polypeptide through the outlets of at least a first zone and a second zone of the HIC apparatus. A residence time for the mixture including the target polypeptide in a first zone may be approximately the same as a residence time of one or more mobile phases in the second zone. 1. A method for preparing a target polypeptide from a mixture including the target polypeptide , the method comprising:contacting the mixture including the target polypeptide to a first zone of a hydrophobic interaction chromatography (HIC) apparatus, the first zone having one or more chromatographic columns and an outlet;contacting one or more mobile phases to a second zone of the HIC apparatus, the second zone having one or more chromatographic columns and an outlet; andpassing the target polypeptide through the outlets of at least the first and second zones of the HIC apparatus;wherein a residence time for the mixture including the target polypeptide in the first zone is configured to be approximately the same as a residence time of the one or more mobile phases in the second zone.2. The method of claim 1 , wherein the target polypeptide is a monoclonal antibody.3. The method of claim 1 , wherein the one or more mobile phases comprises an equilibration buffer and a wash buffer.4. The method of claim 1 , further comprising passing an effluent including the target polypeptide from the first zone of the HIC apparatus to the second zone of the HIC apparatus.5. The method of claim 1 , wherein contacting the one or more mobile phases to the second zone of the HIC apparatus includes:contacting a wash buffer to the second zone of the HIC apparatus; and ...

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07-01-2016 дата публикации

INTEGRATED FLUIDIC CONNECTION OF PLANAR STRUCTURES FOR SAMPLE SEPARATION DEVICES

Номер: US20160003383A1
Принадлежит:

A fluidic device (), comprising a planar structure () constituted by a plurality of laminated layers () and accommodating a fluid channel () extending up to a surface of the planar structure (), and a female adapter piece () configured for a fluid-tight accommodation of a male adapter piece () having a fluid conduit (), wherein the female adapter piece () is connected or connectable with the planar structure () so that, when the male adapter piece () is accommodated in the female adapter piece (), the fluid conduit () is brought in fluid-tight fluid communication with the fluid channel (), wherein the fluid channel () is exposed to the female adapter piece () at a lateral surface of the planar structure () at which the laminated layers () are exposed. 1. A fluidic device , comprising:a planar structure constituted by a plurality of laminated layers and accommodating a fluid channel extending up to a surface of the planar structure);{'b': '206', 'a female adapter piece configured for a fluid-tight accommodation of a male adapter piece having a fluid conduit, wherein the female adapter piece () is connected or connectable with the planar structure so that, when the male adapter piece is accommodated in the female adapter piece, the fluid conduit is brought in fluid-tight fluid communication with the fluid channel;'}wherein the fluid channel is exposed to the female adapter piece at a lateral surface of the planar structure at which the laminated layers are exposed.2. The fluidic device according to claim 1 , wherein the female adapter piece is integrally fixed with the planar structure.3. The fluidic device according to claim 1 , wherein at least part of the laminated layers is patterned to thereby define the fluid channel.4. The fluidic device according to claim 1 , comprising a fluid processing unit located within the planar structure and configured for processing fluid flowing through the fluid channel.5. The fluidic device according to claim 4 , wherein the fluid ...

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03-01-2019 дата публикации

SCALABLE PURIFICATION METHOD FOR AAVRH10

Номер: US20190002841A1
Принадлежит:

A two-step chromatography purification scheme is described which selectively captures and isolates the genome-containing rAAV vector particles from the clarified, concentrated supernatant of a rAAV production cell culture. The process utilizes an affinity capture method performed at a high salt concentration followed by an anion exchange resin method performed at high pH to provide rAAV vector particles which are substantially free of rAAV intermediates 1. A method for separating AAVrh10 viral particles having packaged genomic sequences from genome-deficient AAVrh10 capsid intermediates , said method comprising:subjecting a mixture comprising recombinant AAVrh10 viral particles and AAV rh10 capsid intermediates to fast performance liquid chromatography, wherein the AAVrh10 viral particles and AAVrh10 intermediates are bound to an anion exchange resin equilibrated at a pH of about 10.0 and subjected to a salt gradient while monitoring eluate for ultraviolet absorbance at about 260 and about 280, wherein the AAVrh10 full capsids are collected from a fraction which is eluted when the ratio of A260/A280 reaches an inflection point.2. The method according to claim 1 , wherein the inflection point is when the ratio of A260/A280 changes from less than 1 to greater than 1.3. The method according to claim 1 , wherein the salt gradient has an ionic strength equivalent to at least about 20 mM to about 190 mM NaCl.4. The method according to claim 1 , wherein the AAVrh10 intermediates are separated from the anion exchange resin when the salt gradient reaches an ionic strength equivalent to about 50 nM NaCl or greater.5. The method according to claim 1 , wherein the mixture comprising the recombinant AAVrh10 viral particles and AAVrh10 capsid intermediates contains less than about 10% contamination from viral and cellular proteinaceous and nucleic acid materials.6. The method according to claim 1 , wherein the mixture is at least about 95% purified from viral and cellular ...

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03-01-2019 дата публикации

SCALABLE PURIFICATION METHOD FOR AAV9

Номер: US20190002842A1
Принадлежит:

A two-step chromatography purification scheme is described which selectively captures and isolates the genome-containing rAAV vector particles from the clarified, concentrated supernatant of a rAAV production cell culture. The process utilizes an affinity capture method performed at a high salt concentration followed by an anion exchange resin method performed at high pH to provide rAAV vector particles which are substantially free of rAAV intermediates. 1. A method for separating AAV9 viral particles having packaged genomic sequences from genome-deficient AAV9 capsid intermediates , said method comprising:subjecting a mixture comprising recombinant AAV9 viral particles and AAV9 capsid intermediates to fast performance liquid chromatography, wherein the AAV9 viral particles and AAV9 intermediates are bound to an anion exchange resin equilibrated at a pH of about 10.2 and subjected to a salt gradient while monitoring eluate for ultraviolet absorbance at about 260 and about 280, wherein the AAV9 full capsids are collected from a fraction which is eluted when the ratio of A260/A280 reaches an inflection point.2. The method according to claim 1 , wherein the inflection point is when the ratio of A260/A280 changes from less than 1 to greater than 1.3. The method according to claim 1 , wherein the salt gradient has an ionic strength equivalent to at least about 20 mM to about 190 mM NaCl.4. The method according to claim 1 , wherein the AAV9 intermediates are eluted from the anion exchange resin when the salt gradient reaches an ionic strength equivalent to about 50 nM NaCl or greater.5. The method according to claim 1 , wherein the mixture comprising the recombinant AAV9 viral particles and AAV9 capsid intermediates contains less than about 10% contamination from viral and cellular proteinaceous and nucleic acid materials.6. The method according to claim 1 , wherein the mixture is at least about 95% purified from viral and cellular proteinaceous and nucleic acid materials ...

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03-01-2019 дата публикации

SCALABLE PURIFICATION METHOD FOR AAV1

Номер: US20190002843A1
Принадлежит:

A two-step chromatography purification scheme is described which selectively captures and isolates the genome-containing rAAV vector particles from the clarified, concentrated supernatant of a rAAV production cell culture. The process utilizes an affinity capture method performed at a high salt concentration followed by an anion exchange resin method performed at high pH to provide rAAV vector particles which are substantially free of rAAV intermediates. 1. A method for separating AAV1 viral particles having packaged genomic sequences from genome-deficient AAV1 capsid intermediates , said method comprising:subjecting a mixture comprising recombinant AAV1 viral particles and AAV 1 capsid intermediates to fast performance liquid chromatography, wherein the AAV1 viral particles and AAV1 intermediates are bound to an anion exchange resin equilibrated at a pH of about 9.8 and subjected to a salt gradient while monitoring eluate for ultraviolet absorbance at about 260 and about 280, wherein the AAV1 full capsids are collected from a fraction which is eluted when the ratio of A260/A280 reaches an inflection point.2. The method according to claim 1 , wherein the inflection point is when the ratio of A260/A280 changes from less than 1 to greater than 1.3. The method according to claim 1 , wherein the salt gradient has an ionic strength equivalent to at least about 20 mM to about 190 mM NaCl.4. The method according to claim 1 , wherein the AAV1 intermediates are eluted from the anion exchange resin when the salt gradient reaches an ionic strength equivalent to about 50 nM NaCl or greater.5. The method according to claim 1 , wherein the mixture comprising the recombinant AAV1 viral particles and AAV1 capsid intermediates contains less than about 10% contamination from viral and cellular proteinaceous and nucleic acid materials.6. The method according to claim 1 , wherein the mixture is at least about 95% purified from viral and cellular proteinaceous and nucleic acid materials ...

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03-01-2019 дата публикации

SCALABLE PURIFICATION METHOD FOR AAV8

Номер: US20190002844A1
Принадлежит:

A two-step chromatography purification scheme is described which selectively captures and isolates the genome-containing rAAV vector particles from the clarified, concentrated supernatant of a rAAV production cell culture. The process utilizes an affinity capture method performed at a high salt concentration followed by an anion exchange resin method performed at high pH to provide rAAV vector particles which are substantially free of rAAV intermediates. 1. A method for separating AAV8 viral particles having packaged genomic sequences from genome-deficient AAV8 capsid intermediates , said method comprising:subjecting a mixture comprising recombinant AAV8 viral particles and AAV8 capsid intermediates to fast performance liquid chromatography, wherein the AAV8 viral particles and AAV8 intermediates are bound to an anion exchange resin equilibrated at a pH of about 10.2 and subjected to a salt gradient while monitoring eluate for ultraviolet absorbance at about 260 and about 280, wherein the AAV8 full capsids are collected from a fraction which is eluted when the ratio of A260/A280 reaches an inflection point.2. The method according to claim 1 , wherein the inflection point is when the curve ratio of A260/A280 changes from less than 1 to greater than 1.3. The method according to claim 1 , wherein the salt gradient has an ionic strength equivalent to at least about 20 mM to about 190 mM NaCl.4. The method according to claim 1 , wherein the AAV8 intermediates are eluted from the anion exchange resin when the salt gradient reaches an ionic strength equivalent to about 50 nM NaCl or greater.5. The method according to claim 1 , wherein the mixture comprising the recombinant AAV8 viral particles and AAV8 capsid intermediates contains less than about 10% contamination from viral and cellular proteinaceous and nucleic acid materials.6. The method according to claim 1 , wherein the mixture is at least about 95% purified from viral and cellular proteinaceous and nucleic acid ...

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07-01-2016 дата публикации

SOLUTE EXTRACTING APPARATUS

Номер: US20160003784A1
Принадлежит: MIURA CO., LTD.

A solute trapping column including two trapping agent layers has a first branched pathway extending from an upper part of a main body part thereof, and a second branched pathway extending from between two trapping agent layers, and with its upper part a purification column is connected. By injection of solution of dioxins and supply of solvent into the purification column, dioxins are trapped by the trapping agent layers. An extraction solvent supplied from the lower end side of the solute trapping column while keeping both the purification column and the first branched pathway blocked extracts the dioxins trapped by the second trapping agent layer and flows into the second branched pathway. An extraction solvent supplied in a similar manner while keeping both the purification column and the second branched pathway blocked extracts the dioxins trapped by the first trapping agent layer and flows into the first branched pathway. 1. An apparatus for extracting a solute from a solution , comprising:a solute trapping column having an injection part equipped with an injection port for the solution at one end and a discharge port at the other end, and including multiple trapping agent layers capable of trapping the solute packed with a space interposed therebetween;a first supplying device for supplying the solute trapping column with a solvent for developing the solute within the solute trapping column; anda second supplying device for supplying the solute trapping column with a solvent for extracting the solute,wherein, the solute trapping column has branched pathways, which are independent of each other and respectively extend from the injection part and the space, for the flow of the extraction solvent from the second supplying device, andthe second supplying device is settable during its operation so that the extraction solvent flows in one of the branched pathways sequentially selected from the extraction solvent supply side while flowing of the extraction solvent in ...

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07-01-2021 дата публикации

LIQUID CHROMATOGRAPHY SYSTEMS

Номер: US20210003541A1
Автор: Sievers-Engler Adrian
Принадлежит: ROCHE DIAGNOSTICS OPERATIONS, INC.

A liquid chromatographic (LC) system is introduced which comprises at least one fluidic stream, the fluidic stream comprising a sample-injection valve, a trap-bypass-selection valve, a column-bypass valve, a load-elute valve and a trap-selection valve. Also, a liquid chromatographic (LC) system is introduced which comprises at least one fluidic stream. The fluidic stream comprises a first substream and a second substream. The first substream comprises a first sample-injection valve, a load-elute valve and a trap-selection valve. The second substream comprises a second sample-injection valve and a column-bypass valve. The fluidic stream further comprises a trap-LC substream transfer valve and a substream-selection valve. The LC systems provide a broad choice of chromatographic options and modes and enable to flexibly and rapidly switch between them. 1. A liquid chromatographic (LC) system comprising at least one fluidic stream , the fluidic stream comprising:a sample-injection valve comprising a plurality of ports and a multi-way switch to switch fluidic connections between ports, including a sample input port fluidically connected to a sample input nozzle, an aspiration/dispensing-pump port fluidically connected to a sample aspiration pump, a sample-loop-input port and a sample-loop-output port interconnected by a sample loop, an LC-pump port fluidically connected to an LC pump and a sample-injection-to-trap-bypass-selection port;a trap-bypass-selection valve comprising a plurality of ports and a multi-way switch to switch fluidic connections between ports, including a trap-bypass-selection-to-sample-injection port fluidically connected to the sample-injection-to-trap-bypass-selection port of the sample injection valve, two bypass ports interconnected by a bypass fluidic path, a trap-bypass-selection-to-column-bypass port and two trap-bypass-selection-to-load-elute ports;a column-bypass valve comprising a plurality of ports and a multi-way switch to switch fluidic ...

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07-01-2021 дата публикации

METHODS FOR EXTRACTING PROTEINS FROM BLOOD PLASMA

Номер: US20210003552A1
Принадлежит:

A method of producing protein products including alpha-1-proteinase inhibitor, gamma globulin, albumin, and other proteins from plasma includes steps of: (1) adding a salt to the blood product to produce a first intermediate, wherein the salt comprises between 11-13 wt % of the first intermediate; (2) separating the first intermediate to produce a first supernatant and a first paste; (3) adding a salt to the first intermediate to produce a second intermediate, wherein the salt comprises between 21-23 wt % of the second intermediate; (4) separating the second intermediate to produce a second supernatant and a second paste; (5) separating a third intermediate from the second supernatant by affinity chromatography; and (6) separating the third intermediate by ion exchange chromatography to produce an eluate containing the protein product. Advantageously, the inventive methods are simple and produce alpha-1-proteinase inhibitor, gamma globulin, albumin, and other proteins in high yields. 1. A method of separating A1P1 from a blood plasma containing product , the method comprising:thawing a frozen blood plasma product followed by stirring at a temperature suitable for dissolving a cryoprecipitate to generate the blood plasma containing product;adding a salt to the blood plasma containing product to produce a first intermediate, wherein the salt comprises between 11-20 wt % of the first intermediate;separating the first intermediate to produce a first supernatant and a first paste;adding a salt to the first supernatant to produce a second intermediate, wherein the salt comprises between 15-30 wt % of the second intermediate;separating the second intermediate to produce a second supernatant and a second paste;separating the second supernatant by affinity chromatography using an A1P1-specific affinity media to generate a flow-through fraction and a first eluate, wherein the first eluate comprises A1P1.2. The method of claim 1 , wherein the step of adding the salt to the ...

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13-01-2022 дата публикации

METHODS OF PURIFICATION OF ALBUMIN FUSION PROTEINS

Номер: US20220009958A1
Автор: Bogsnes Are, Dolby Viveka
Принадлежит:

The present invention provides a chromatographic separation method for improving the quality of albumin fusion protein solutions by removing impurities from the albumin fusion protein solution. This invention provides albumin fusion protein solution with a significantly reduced amount of the (yellow) coloured impurities and HCP. 1. A method of purification of an aqueous albumin fusion protein solution , wherein said method comprises: (i) the step of loading said fusion protein solution onto a column comprising a cationic exchange chromatographic resin , and (ii) the step of eluting the fusion protein from the column using an elution buffer with a cation containing gradient.2. The method according to claim 1 , wherein the albumin fusion protein is a MIC-1 human serum albumin fusion protein.3. The method according to claim 1 , wherein the cation containing gradient is a Na claim 1 , Mg NH claim 1 , K or Ca containing gradient.4. The method according to claim 3 , wherein the cation containing gradient is a Ca containing gradient.5. The method according to claim 1 , wherein the elution buffer has a pH of 4.0-5.0.6. The method according to claim 5 , wherein the elution buffer has a pH of 4.2.7. The method according to claim 1 , wherein the albumin fusion protein is eluted from the cation exchange column using an elution buffer gradient comprising 20 mM acetic acid claim 1 , 11 mM NaOH and 500 mM CaCl) claim 1 , at a pH of 4.2 and gradually increasing the content of CaCl) in the elution buffer to 500 mM at pH 4.2.8. The method according to claim 1 , further comprising carrying out an anion exchange purification step of the albumin fusion protein solution prior to step (i).9. The method according to claim 8 , wherein the albumin fusion protein is eluted from an anion exchange column in a Tris buffered sodium chloride solution at pH of 7.7 claim 8 , following a wash comprising ethanol and Ca.10. The method according to claim 1 , further comprising claim 1 , after step (ii) ...

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03-01-2019 дата публикации

MODULAR AUTOMATED CHROMATOGRAPHY SYSTEM

Номер: US20190004017A1
Принадлежит:

Valves, pumps, detectors, sample loops, fraction collectors and the like are individually incorporated into modules that are mountable at individual mounting sites on a base unit which also supports one or more chromatography columns. Each module includes fluid connections to other modules and a microcontroller joining the module to a computed and monitor through an electronic connector at each mounting site. The fluid connections between the modules and the column(s) are removed from the electronic connections and accessible to the user. A software platform may recognize the modules and their locations, coordinate fluid connections between the modules, and provide a variety of control, monitoring, data generating and data processing functions to generate chromatographic data. The software platform may also provide graphical tools for designing chromatographic methods from a library of phases. 1. A system for joining a plurality of fluid manipulation components into a flow scheme for directing fluids to and from a chromatographic separation device and for operating said joined fluid manipulation components according to a selected protocol , said system comprising:a plurality of modules, each said module comprising (a) one of said fluid manipulation components, and (b) a microcontroller that transmits operational signals to said fluid manipulation component in response to commands received from outside said module;a mounting frame comprising a plurality of bays, each of the plurality of bays constructed to receive a single module, and each of the plurality of bays comprising a signal connector that couples to and communicates with the microcontroller of a module mounted in said bay, wherein at least some of the bays are constructed to receive single modules interchangeably with other said modules; anda software platform in communication with the microcontroller of each module, said software being programmed to (1) receive from each of the plurality of modules ...

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13-01-2022 дата публикации

METHOD FOR PREPARING AT LEAST ONE GENERATOR WITH A HIGH RADIUM-228 CONTENT

Номер: US20220013246A1
Принадлежит:

A method for preparing one or more generators with a high radium-228 content from an aqueous solution comprising thorium-232 and radium-228. The generator(s) can be used, in particular, for producing thorium-228, from which radium-224, then lead-212 and bismuth-212 can be obtained. The method and the generator(s) that it can be used to prepare are therefore applicable, in particular, in the manufacture of radiopharmaceuticals made from lead-212 or bismuth-212, which can be used in nuclear medicine and, in particular, in targeted alpha radiotherapy for the treatment of cancers. 1. A method for preparing at least one generator comprising radium-228 from an aqueous solution A1 comprising thorium-232 and radium-228 , comprising at least the steps of:a) circulating in a first chromatography column a volume V1 of the aqueous solution A1, the first chromatography column comprising a first stationary phase consisting of a solid material which selectively retains radium with respect to thorium;b) washing at least once the first stationary phase with an aqueous solution A2;c) eluting the radium-228 from the first stationary phase with a volume V3 of an aqueous solution A3 comprising an agent complexing radium-228, the volume V3 being between 0.005% and 1% of the volume V1 of the aqueous solution A1 having circulated in the first chromatography column, whereby an aqueous solution A4 which comprises radium-228 complexes is obtained;d) dissociating the radium-228 complexes present in the aqueous solution A4 by modifying a pH of the aqueous solution A4, whereby an aqueous solution A5 comprising the radium-228 in a decomplexed form is obtained;e) loading a second chromatography column with the aqueous solution A5, the second chromatography column comprising a second stationary phase consisting of a same material as the first stationary phase; andf) washing at least once the second stationary phase with an aqueous solution A6, whereby the at least one generator is obtained.2. The ...

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08-01-2015 дата публикации

PNEUMATICALLY/HYDRAULICALLY ACTUATED FLUOROPOLYMER-HPLC CHROMATOGRAPHIC SYSTEM FOR USE WITH HARSH REAGENTS

Номер: US20150008171A1
Принадлежит:

The invention provides a high-performance liquid chromatography system, said system is controlled in temperature by running a fluid in sleeves that surround the different parts of the system. All parts in contact with the fluid are made in fluoropolymer, carbon-filled fluoropolymer, or carbon-fiber fluoropolymer. The system comprises at least one reagent reservoir; at least one mixing chamber, wherein the contents of the at least one reagent reservoir are combined; at least one pump that transfers the contents of the at least one reservoir to the mixing chamber; and at least one modular elution column, wherein the at least one modular elution column contains a temperature control means; a sample injection system connected to an injection loop or 3-way valve to inject the sample solutions in the modular elution columns, at least one manifold or X-Y moving stage to distribute the eluted volumes in different receptacles; at least one return line to automatically reinject selected elution fractions at the top of the column; wherein all moving components of the said system are fluid actuated. 1. A high-performance liquid chromatography system wherein all moving components of that said system are actuated by fluid pressure , said system comprising:a. at least one reagent reservoir, wherein the at least one reservoir contains a temperature control means;b. a mixing chamber, wherein the contents of the at least one reagent reservoir are combined, and wherein the mixing chamber contains a temperature control means;c. a pump that transfers the contents of the at least one reservoir to the mixing chamber;d. a modular elution column, wherein the column contains a temperature control means;e. a means for injecting one or a plurality of sample solutions in the modular elution column;f. a means for distributing eluted volumes to different receptacles; wherein all moving components of the said system are pneumatically actuated andg. a return line with a pump to automatically reload ...

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08-01-2015 дата публикации

HG SENSOR USING ANISOTROPIC AU NANOPARTICLES AND RELATED WATER REMEDIATION

Номер: US20150008174A1

A method of sensing Hg and related Hg sensing system for fluid samples includes the steps of providing a sensing solution including a plurality of anisotropic Au nanoparticles, and contacting a water sample or an air sample suspected of containing Hg or a vapor stream derived from the water sample with the plurality of anisotropic Au nanoparticles. A gold amalgam compound is generated when Hg is present in the sample. The presence, and optionally the concentration, of Hg in the sample are then determined using an optical method based on a change in at least one of absorption, reflectance and scattering of the solution. In a related inventive embodiment a filter for water treatment and remediation including the removal of Hg includes a first flow through grid having a Hg reducing material thereon on an inlet side of the filter and at least one flow through second grid including a surface having amalgamating material downstream from the first grid. 1. A method of sensing Hg in fluids , comprising the steps of:providing a sensing solution including a plurality of anisotropic Au nanoparticles;contacting a fluid sample suspected of containing Hg with said sensing solution; anddetermining if Hg is present in said sample using an optical method, said optical method comprising detecting a change in an optical parameter resulting from said contacting step.2. The method of claim 1 , wherein claim 1 , said change comprises a spectral change of at least one of absorption claim 1 , reflectance and scattering.3. The method of claim 1 , wherein the step of determining includes quantification of said Hg in said sample.4. The method of claim 1 , wherein said change comprises a shift in a maximum absorption wavelength.5. The method of claim 4 , wherein said shift comprises a shift in a longitudinal mode band of said anisotropic Au nanoparticles.6. The method of claim 1 , wherein said fluid sample comprises a water sample claim 1 , an air sample claim 1 , or a vapor stream derived ...

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14-01-2021 дата публикации

FILTER ASSEMBLY AND PORTABLE WATER FEEDER

Номер: US20210007324A1
Автор: CHEN Silong
Принадлежит:

The present invention relates to water feeder technical field, more particularly to a filter assembly and a portable water feeder. The filter assembly includes a tray and a filter piece; the tray comprises a bottom frame with a bottom plate and a supporting wall and a supporting ring, the supporting wall is arranged around its circumference of the bottom plate and extends upward, the supporting wall and the bottom plate are jointly surrounded to form a cavity, and the bottom plate is provided with a number of filter holes; the supporting ring is arranged above the bottom frame and is connected to its top of the supporting wall, the filter piece is placed in the cavity. When the filter assembly is used, water flows from the filter hole into the filter piece, and the filter assembly has the advantages of simple and compact structure. 1. A filter assembly , wherein including a tray and a filter piece; said tray comprising:a bottom frame, which includes a bottom plate and a supporting wall, said supporting wall is arranged around its circumference of said bottom plate and extends upward, and said supporting wall and said bottom plate are jointly surrounded to form a cavity, and said bottom plate is provided with a number of filter holes; anda supporting ring, which is arranged above said bottom frame, and said supporting ring is connected to its top of said supporting wall and, said filter piece is placed in said cavity.2. The filter assembly according to claim 1 , wherein its middle of said bottom plate is provided with a central hole with an aperture of 13 mm-17 mm claim 1 , and its inner wall of said central hole is provided an arc surface smoothly transiting to its bottom edge and its top edge of said central hole.3. The filter assembly according to claim 1 , wherein said filter hole is a strip-like structure extended from said bottom plate to said supporting wall claim 1 , and its width of said filter hole is gradually increased from said bottom plate to said ...

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11-01-2018 дата публикации

System for Blending Solutions

Номер: US20180008907A1
Автор: Olovsson Bjorn Markus
Принадлежит:

A system for blending solutions and a buffer solution is disclosed. In this system a switch valve is present capable of flowing one or more solutions, a low pressure pump for pumping the one or more solutions through the switch valve and a T-joint capable of receiving the one or more solutions through the low pressure pump and blending the one or more solutions with a buffer solution. A high pressure pump is present for collecting a blended solution. 1. A system for blending solutions , the system comprising:a switch valve capable of flowing at least one solution;a low pressure pump for pumping the at least one solution through the switch valve;a T-joint placed between the low pressure pump and high pressure pump, the T-joint capable of receiving the at least one solution through the low pressure pump and blending the at least one solution with a buffer solution; anda high pressure pump for collecting a blended solution.2. The system of claim 1 , wherein the T-joint is connected to a container holding the buffer solution through at least one direct connection.3. The system of claim 2 , wherein the switch valve is capable of allowing the buffer solution to flow from the container claim 2 , the low pressure pump enable pumping of the buffer solution through the switch valve.4. The system of claim 1 , wherein the at least one solution comprises at least one of an acid claim 1 , a base and a salt solution.5. The system of claim 1 , further comprising a control system configured to: control operation of the low pressure pump for supplying the at least one solution and control the functioning of the switch valve capable of flowing the at least one solution.6. The system of claim 5 , wherein the control system is configured to control the flow rate of the at least one solution supplied by the at least one low pressure pump claim 5 , the flow rate of the at least one solution determines concentration of the blended solution.7. The system of claim 1 , further comprising at ...

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11-01-2018 дата публикации

Purification and Separation System and Method

Номер: US20180008908A1
Автор: Allison Justin
Принадлежит:

A purification system for separating a molecule of interest from a solution is provided and generally includes a plurality of resin processing sections, wherein each of the resin processing sections is controllably separated from each other via a configurable gate valve, wherein the configurable gate valve is configured to introduce or evacuate a fluid/resin into/out of a proximately located processing section, a plurality of inlets configured to introduce fluid and/or resin into one or more of the plurality of resin processing sections. 1a configurable gate valve;a plurality of inlets; anda plurality of resin processing sections, wherein each of the resin processing sections is controllably separated from each other via the configurable gate valve, wherein the configurable gate valve is configured to introduce or evacuate a fluid/resin into/out of a proximately located processing section, and wherein the plurality of inlets are configured to introduce fluid and/or resin into one or more of the plurality of resin processing sections.. A purification system for separating a molecule of interest from a solution, the system comprising: This application claims the benefit of the filing date of U.S. Provisional Patent Application Ser. No. 61/810,826 filed Apr. 11, 2013 and titled “Purification and Separation System and Method,” the contents of which are incorporated by reference herein in its entirety.This invention relates generally to molecular purification and more particularly to a system for biologically produced molecular purification using a mass transfer technique and article.Molecular purification devices for purifying polypeptides, proteins, polysaccharides, nucleic acids and molecules are known and typically use a traditional chromatography or other established approach. These devices include chromatography columns and include a fixed bed of particles typically consisting of chemically active ligands that are covalently bonded to matrices of polysaccharides, ...

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10-01-2019 дата публикации

Purification Process

Номер: US20190009206A1
Принадлежит:

A process is described for removing halogen compounds, particularly chlorine compounds, from a process fluid, comprising the steps of (i) passing a process fluid containing hydrogen halide over a first sorbent to remove hydrogen halide and generate a hydrogen halide depleted process fluid and then, (ii) passing the hydrogen halide depleted process fluid over a second different sorbent to remove organic halide compounds therefrom. A purification system suitable for removing hydrogen halide and organic halide compounds from process fluids is also described. 1. A process for removing a halogen compound from a process fluid , comprising the steps of:(i) passing a process fluid containing hydrogen halide over a first sorbent comprising an alkalized alumina to remove hydrogen halide and generate a hydrogen halide depleted process fluid; and(ii) passing the hydrogen halide depleted process fluid over a second sorbent comprising zeolite 13X to remove an organic halide compound.2. The process of claim 1 , wherein the process fluid is a hydrogen gas stream comprising 50% vol or greater of hydrogen.3. The process of claim 1 , wherein the process fluid is a liquid stream comprising a hydrocarbon or a gas stream comprising a hydrocarbon.4. The process of claim 1 , wherein the process fluid is a liquid stream comprising a hydrocarbon.5. The process of claim 1 , wherein the halogen compound is a bromine compound or a chlorine compound.6. The process of claim 5 , wherein the halogen compound is a chlorine compound.7. The process of claim 1 , wherein the hydrogen halide content of the process fluid fed to the first sorbent is in the range of from 0.1 to 20 ppm.8. The process of claim 1 , wherein the first sorbent comprises acidic sites that form one or more organic halide compounds.9. The process of claim 1 , wherein the process fluid from step (ii) is passed over a third sorbent to remove residual or formed hydrogen halide.10. The process of claim 9 , wherein the third sorbent is ...

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10-01-2019 дата публикации

ISOTOPE PREPARATION METHOD

Номер: US20190009265A1
Принадлежит:

The present invention comprises a method for the generation of Th of pharmaceutically tolerable purity comprising i) preparing a generator mixture comprising Ac, Th and Ra; ii) loading said generator mixture onto a strong base anion exchange resin; iii) eluting a mixture of said Ra and Ac from said strong base anion exchange resin using a first mineral acid in an aqueous solution; iv) eluting Th from said strong base anion exchange resin using a second mineral acid in an aqueous solution whereby to generate a first Th solution containing contaminant Ra and Ac; v) loading the first Th solution onto a strong acid cation exchange resin; vi) eluting at least a part of the contaminant Ra and Ac from said strong acid cation exchange resin using a third mineral acid in aqueous solution; and vii) eluting the Th from said strong acid cation exchange resin using a first aqueous buffer solution to provide a second Th solution. Purified thorium-227 of pharmaceutical purity and a pharmaceutical composition comprising the same are also provided. 1) A method for the generation of Th of pharmaceutically tolerable purity comprising the steps of:{'sup': 227', '227', '223, 'i) preparing a generator mixture comprising Ac, Th and Ra;'}ii) loading said generator mixture onto a strong base anion exchange resin;{'sup': 223', '227, 'iii) eluting a mixture of said Ra and Ac from said strong base anion exchange resin using a first mineral acid in an aqueous solution;'}{'sup': 227', '227', '223', '227, 'iv) eluting Th from said strong base anion exchange resin using a second mineral acid in an aqueous solution whereby to generate a first Th solution containing contaminant Ra and Ac;'}{'sup': '227', 'v) loading the first Th solution onto a strong acid cation exchange resin;'}{'sup': 223', '227, 'vi) eluting at least a part of the contaminant Ra and Ac from said strong acid cation exchange resin using a third mineral acid in aqueous solution; and'}{'sup': 227', '227, 'vii) eluting the Th from ...

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11-01-2018 дата публикации

Xylene separation process

Номер: US20180009729A1

A process is described for separating paraxylene from a multicomponent fluid mixture of C8 aromatics. A mixture of C8 aromatics is fed to a simulated moving-bed adsorptive apparatus. The location of the feed to the apparatus is moved at set intervals. The rate of flow of feed to the apparatus is varied during each interval to enhance the separation of paraxylene from the multicomponent mixture.

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09-01-2020 дата публикации

A Modular Bio-Processing Unit and a Bio-Processing System Employing Plural Units

Номер: US20200009557A1
Принадлежит:

Disclosed is a modular bio-processing unit () comprising: a housing () having one or more internal fluid paths (), the housing having at least one inlet and at least one outlet (), each in fluid communication with the fluid path or one or more of the fluid paths; one or more sensor elements () operatively associated with the or each path, said sensor(s) elements including elements of one or more of: a flow sensor, a flow rate sensor, a conductivity sensor, a pressure sensor, a pH sensor, and a light absorbance sensor such as a UV spectroscopic concentration sensor; one or more fluid flow inducing components () operatively associated with the or each fluid path; and plural valves () for preventing or reducing flow in the or each path, the housing, inlet(s), outlet(s), flow inducing component(s) and valve(s) being arranged to operate together as a bio-processing unit within or substantially within the housing. 1. A modular bio-processing unit operable with like units to provide a bio-processing system , comprising:a housing having one or more internal fluid paths, the housing having at least one inlet and at least one outlet, each in fluid communication with the fluid path or one or more of the fluid paths;one or more sensor elements operatively associated with the or each path, said sensor(s) elements including elements of one or more of: a flow rate or flow direction sensor, a conductivity sensor, a pressure sensor, a pH sensor, an air trap and a light absorbance sensor such as a UV light absorbance sensor;one or more fluid flow inducing components operatively associated with the or each fluid path; andplural valves for preventing or reducing flow in the or each path, operate together as a bio-processing unit within or substantially within the housing.2. The modular bio-processing unit of claim 1 ,wherein the unit includes a selection valve for selecting input into the path(s) from plural sources.3. The modular bio-processing unit of claim 2 ,wherein the flow ...

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14-01-2021 дата публикации

FILTER FOR WATER PURIFIER AND WATER PURIFIER INCLUDING THE SAME

Номер: US20210009442A1
Принадлежит:

A filter for a water purifier includes a filter housing that defines an inlet and an outlet, and a filter module disposed inside the filter housing and configured to purify water received through the inlet and supply purified water to the outlet. The filter module includes a carbon block that includes a mixture of: activated carbon having a weight corresponding to 40 to 50% of a weight of the mixture, a binder having a weight corresponding to 5 to 15% of the weight of the mixture, iron hydroxide having a weight corresponding to 10 to 20% of the weight of the mixture, and titanium oxide having a weight corresponding to 30 to 40% of the weight of the mixture. 1. A filter for a water purifier , the filter comprising:a filter housing that defines an inlet and an outlet; and activated carbon having a weight corresponding to 40 to 50% of a weight of the mixture,', 'a binder having a weight corresponding to 5 to 15% of the weight of the mixture,', 'iron hydroxide having a weight corresponding to 10 to 20% of the weight of the mixture, and', 'titanium oxide having a weight corresponding to 30 to 40% of the weight of the mixture., 'a filter module disposed inside the filter housing and configured to purify water received through the inlet and supply purified water to the outlet, the filter module comprising a carbon block that comprises a mixture of2. The filter of claim 1 , wherein the binder comprises polyethylene (PE).3. The filter of claim 2 , wherein the carbon block has a hollow tube shape.4. The filter of claim 3 , further comprising a non-woven fabric that is made of a resin and that covers an outer surface of the carbon block.5. The filter of claim 3 , wherein a ratio of an inner diameter of the carbon block to an outer diameter of the carbon block is 1:3 to 1:4.6. The filter of claim 1 , wherein the titanium oxide comprises titanium dioxide.7. A filter for a water purifier claim 1 , the filter comprising:a filter housing that defines an inlet and an outlet; anda ...

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12-01-2017 дата публикации

MULTIPLE COLUMN CHROMATOGRAPHIC SYSTEM AND METHODS OF USE

Номер: US20170010243A1
Принадлежит:

A chromatographic apparatus includes two chromatographic columns configured to perform chromatography. The apparatus further includes a primary valve disposed upstream of the first chromatographic column and the second chromatographic column. The primary valve selectively switches between a first position in which the pump is in fluid communication with a first path that includes the first chromatographic column and a second position in which the pump is in fluid communication with a second path that includes the second chromatographic column. The apparatus also includes a first and a second sample injection port associated with said first and second columns, respectively, and at least one detector. The apparatus is configured such that flow from the column in the selected path goes to said at least one detector without passing through the other column. 1. A chromatographic apparatus comprising:a first chromatographic column configured to perform a first type of chromatography;a second chromatographic column configured to perform a second type of chromatography;a pump configured to be in selective fluid communication with either the first chromatographic column or the second chromatographic column, the pump being upstream of the columns; a first position in which the pump is in fluid communication with a first path that includes the first chromatographic column; and', 'a second position in which the pump is in fluid communication with a second path that includes the second chromatographic column;, 'a primary valve disposed upstream of the first chromatographic column and the second chromatographic column and downstream of the pump, the primary valve configured to selectively switch between'}a first and a second sample injection port associated with said first and second columns, respectively; andat least one detector downstream of said columns;wherein the apparatus is configured such that flow from the column in the selected path goes to said at least one detector ...

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14-01-2021 дата публикации

IL-15/IL-15RALPHA BASED CONJUGATES PURIFICATION METHOD

Номер: US20210009658A1
Принадлежит:

The present invention relates to a method for preparing a composition comprising monomeric conjugates from a sample, said conjugate comprising (a) a polypeptide comprising the amino acid sequence of interleukin 15 or derivatives thereof, and (b) a polypeptide comprising the amino acid sequence of the sushi domain of IL-15Rα or derivatives thereof; wherein said method comprises the use of anion-exchange chromatography followed by a hydrophobic interaction chromatography; and to a pharmaceutical composition which can be obtained by such a method. 114.-. (canceled)16. The pharmaceutical composition of claim 15 , wherein the mammalian cell comprises CHO cells.17. The pharmaceutical composition of claim 15 , wherein the polypeptide a) consists of the amino acid sequence of interleukin 15 set forth in SEQ ID NO: 1 claim 15 , and the polypeptide b) consists of the amino acid sequence of the sushi domain of IL-15Rα set forth in SEQ ID NO: 4.18. The pharmaceutical composition of claim 15 , wherein the polypeptide a) consists of the amino acid sequence of human interleukin 15 set forth in SEQ ID NO: 3 claim 15 , and the polypeptide b) consists of the amino acid sequence of the human sushi domain of IL-15Rα set forth in SEQ ID NO: 8.19. The pharmaceutical composition of claim 15 , wherein the conjugate is selected from the conjugates set forth in SEQ ID NO: 16 and SEQ ID NO: 17.20. The pharmaceutical composition of claim 15 , wherein the pharmaceutically acceptable carrier is a solvent claim 15 , adjuvant claim 15 , excipient claim 15 , or vehicle.21. The pharmaceutical composition of claim 15 , wherein the composition is used for treating a cancer claim 15 , an infection and/or an immunodeficiency disorder in a subject. The present international patent application claims the priority of European patent application EP 14000742.8 filed on Mar. 3, 2014, which is herein incorporated by reference.The present invention relates to the field of biochemical engineering. More ...

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10-01-2019 дата публикации

PROCESS FOR PURIFICATION AND SEPARATION OF CANNABINOIDS, FROM DRIED HEMP AND CANNABIS LEAVES

Номер: US20190010106A1
Принадлежит:

Disclosed is a method for purification and separation of cannabinoids, specifically cannabidiol and tetrahydrocannabinol, from the dried hemp and leaves using a combination of a sequence of purification steps including: filtration, decolorization, activation or decarboxylation, dewaxing, a continuous simulated moving bed process, polishing, and crystallization to separate cannabinoids from tetrahydrocannabinol and to provide phytocannabinoid rich oil and cannabidiol isolate. The cannabinoid products can be used in various pharmaceutical and nutraceutical applications. 1cannabis. A process for the purification of cannabidiol (CBD) in a crude extract stream to provide at least one high purity cannabidiol product selected from the group consisting of a high purity cannabinoid oil stream , a phytocannabinoid rich oil , a solid CBD aggregate and mixtures thereof being essentially free of tetrahydrocannabinol , said process comprising:{'i': 'cannabis', 'a) passing the crude extract stream comprising debris and small particles, cannabidiol, tetrahydrocannabinol, cannabidiolic acid, tetrahydrocannabinolic acid, other cannabinols, chlorophylls, color bodies, sugars and carbohydrates, lipids, plant waxes, impurities, and ethanol to a first filtration zone comprising a series of successive filters of decreasing pore size, starting at a pore size of 100 microns and reducing to about 10 microns in 3 or more stages to remove debris and small particles in a progressive filtration step to provide a filtered crude cannabinoid stream;'}b) passing the filtered crude cannabinoid stream comprising cannabidiol, tetrahydrocannabinol, cannabidiolic acid, tetrahydrocannabinolic acid, other cannabinols, chlorophylls, color bodies, sugars and carbohydrates, lipids, plant waxes, impurities, and ethanol to a decolorization zone comprising a 10 μm filter and a decolorization chromatographic column containing a modified activated carbon adsorbent which was heat treated to provide a highly ...

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10-01-2019 дата публикации

PROCESS FOR PURIFICATION AND SEPARATION OF CANNABINOIDS, FROM DRIED HEMP AND CANNABIS LEAVES

Номер: US20190010107A1
Принадлежит: OROCHEM TECHNOLOGIES, INC.

A method for purification and separation of cannabinoids, such as cannabidiol and tetrahydrocannabinol, e.g., from the dried hemp and leaves can use a continuous simulated moving bed process and a combination of one or more of a sequence of purification steps including: filtration, decolorization, activation or decarboxylation, dewaxing, polishing, and crystallization to separate a cannabinoid from the plant and to provide various cannabinoid products. The cannabinoid products can be used in various pharmaceutical and nutraceutical applications. 1cannabiscannabis. A method of separating a cannabinoid from a plant , the plant including the cannabinoid and at least one impurity , the method comprising:{'i': cannabis', 'cannabis, 'combining the plant and a solvent to form a crude extract stream;'}{'i': cannabis', 'cannabis, 'processing the crude extract stream into a simulated moving bed (SMB) feedstock stream by removing at least a portion of at least one impurity in the crude extract stream; and'}passing the SMB feedstock stream through a SMB zone to provide a primary raffinate stream having a higher purity of the cannabinoid than in the SMB feedstock stream as measured by weight percentage of the solid content and a SMB extract stream having a lower purity of the cannabinoid than in the SMB feedstock stream as measured by weight percentage of the solid content.2cannabisCannabis sativa, Cannabis indica, Cannabis rudralis. The method of claim 1 , wherein the plant is selected from claim 1 , and mixtures thereof.3cannabiscannabis. The method of claim 1 , wherein the plant comprises dried hemp claim 1 , leaves claim 1 , or a mixture thereof.4. The method of claim 1 , wherein the solvent comprises ethanol.5cannabiscannabiscannabiscannabiscannabiscannabiscannabiscannabiscannabis. The method of claim 1 , wherein said at least one impurity comprises at least one of color bodies claim 1 , acidic components claim 1 , lipids claim 1 , and plant waxes claim 1 , and wherein ...

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10-01-2019 дата публикации

PROCESS FOR SEPARATING A CONSTITUENT/CANNABINOID USING A CHROMATOGRAPHIC RESIN

Номер: US20190010110A1
Принадлежит: OROCHEM TECHNOLOGIES, INC.

A method for purification and separation of cannabinoids, such as cannabidiol and tetrahydrocannabinol, e.g., from dried hemp and leaves can use a continuous simulated moving bed process, a batch column chromatography method, or a single column, and a combination of one or more of a sequence of purification steps including: filtration, decolorization, activation or decarboxylation, dewaxing, polishing, and crystallization to separate a cannabinoid from the plant and to provide various cannabinoid products. The cannabinoid products can be used in various pharmaceutical and nutraceutical applications. 1cannabiscannabis. A method of separating a cannabinoid of a plant , the plant including the cannabinoid and at least one impurity , the method comprising:{'i': 'cannabis', 'preparing a feedstock stream, the feedstock stream including the plant and a solvent;'} a first resin, the first resin comprising a modified activated carbon adsorbent having an average particle size range of from about 45 to about 1700 microns,', 'a second resin, the second resin comprising a modified hydrophobic adsorbent having an average bulk density of from about 0.4 g/mL to about 0.6 g/mL, the modified hydrophobic adsorbent comprising at least one of a styrene-divinylbenzene (DVB) resin or a poly(methyl methacrylate) (PMMA) resin,', 'a third resin, the third resin comprising a hydrophobic resin having an average bulk density of from about 0.75 g/mL to about 0.85 g/mL,', 'a fourth resin, the fourth resin comprising a hydrophobic polystyrene-divinylbenzene adsorbent having a water content of from about 55% to about 65%, and', 'any mixture thereof., 'passing the feedstock stream through a chromatographic resin to provide an eluate stream, the eluate stream having a higher purity of the cannabinoid than in the feedstock stream as measured by weight percentage of the solid content, wherein the chromatographic resin comprises at least one of2. The method of claim 1 , wherein the cannabinoid is CBD ...

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14-01-2021 дата публикации

Separation and Quantification of Empty and Full Viral Capsid Particles

Номер: US20210009964A1
Принадлежит: Sangamo Therapeutics, Inc.

The present disclosure provides methods for the separation and quantification of empty and full viral capsids (e.g., AAV capsids) within a viral preparation, such as a viral pharmaceutical composition or drug product. 1. A method of separating empty and full capsids in a viral preparation , the method comprising running the viral preparation and a mobile phase through an anion exchange column , wherein the mobile phase is run under conditions comprising a discontinuous elution gradient and at least one isocratic hold.2. A method of quantitating empty and full capsids in a viral preparation , the method comprising running the viral preparation and a mobile phase through an anion exchange column , wherein the mobile phase is run under conditions comprising a discontinuous elution gradient and at least one isocratic hold.3. The method of claim 1 , wherein the viral preparation and mobile phase are run on a high-performance liquid chromatography (HPLC) system.4. The method of claim 1 , wherein the empty and full capsids are separated by baseline resolution.5. The method of claim 4 , wherein the baseline resolution is greater than 2.0.6. The method of claim 1 , wherein the capsids comprise adeno-associated virus (AAV) capsids.7. The method of claim 1 , wherein the anion column is a strong anion exchange (SAX) column.8. The method of claim 7 , wherein the SAX column is a quaternary amine (Q-amine) column.9. The method of claim 1 , wherein the anion exchange column is a monolith column.10. The method of claim 1 , wherein the mobile phase comprises a salt.11. The method of claim 10 , wherein the salt is tetramethylammonium chloride (TMAC) or sodium acetate.12. The method of claim 10 , wherein the final gradient of the mobile phase comprises 0.5 to 5 M claim 10 , optionally 1 M claim 10 , salt.13. The method of claim 1 , wherein the at least one isocratic hold is introduced before the mobile phase reaches 50% of the final gradient.14. The method of claim 1 , wherein the pH ...

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14-01-2016 дата публикации

METHOD FOR FRACTIONATING DIOXINS

Номер: US20160011163A1
Принадлежит: MIURA CO., LTD.

The interior of a fractionating tool for fractionating dioxins is packed with a purification layer and an adsorption layer. The adsorption layer includes a first adsorption layer including an activated carbon-containing silica gel layer and a graphite-containing silica gel layer, and a second adsorption layer including an alumina layer. When a solution of dioxins is injected into the purification layer and is supplied with an aliphatic hydrocarbon solvent, the solvent dissolves dioxins in the solution of dioxins and passes through the purification layer and the adsorption layer. In this process, non-ortho PCBs, PCDDs and PCDFs among dioxins are adsorbed to the first adsorption layer, and mono-ortho PCBs among dioxins are adsorbed to the second adsorption layer. As a result, dioxins are fractionated into a group including non-ortho PCBs, PCDDs and PCDFs, and mono-ortho PCBs. 1. A method for fractionating dioxins , comprising the step of:passing an aliphatic hydrocarbon solvent solution of dioxins through an activated carbon-containing silica gel layer and a graphite-containing silica gel layer in this order.2. The method for fractionating dioxins according to claim 1 , wherein the aliphatic hydrocarbon solvent solution having passed through the graphite-containing silica gel layer is further passed through an alumina layer.3. The method for fractionating dioxins according to claim 2 , further comprising the steps of:supplying the activated carbon-containing silica gel layer and the graphite-containing silica gel layer through which the aliphatic hydrocarbon solvent solution has passed with a solvent capable of dissolving dioxins, to secure the solvent having passed through the activated carbon-containing silica gel layer and the graphite-containing silica gel layer; andsupplying the alumina layer through which the aliphatic hydrocarbon solvent solution has passed with a solvent capable of dissolving dioxins, to secure the solvent having passed through the alumina ...

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09-01-2020 дата публикации

A PROCESS FOR PURIFICATION OF POLYETHER BLOCK COPOLYMERS

Номер: US20200010618A1
Принадлежит:

Process for providing purified polyether block copolymers comprising polyoxyethylene (PEO) and polyoxypropylene (PPO) moieties wherein the purified product is obtained by an ultrafiltration step of a solution of the polyether block copolymers and wherein the block copolymers are depleted in lower molecular impurities. 1. A process for providing purified polyether block copolymers comprising polyoxyethylene (PEO) and polyoxypropylene (PPO) moieties wherein the purified polyether block copolymer is obtained by an ultrafiltration step of a solution of the polyether block copolymer.2. The process according to claim 1 , wherein the polyether block copolymers comprising polyoxyethylene and polyoxypropylene moieties are selected from the group consisting of polyethylene oxide-block-polypropylene oxide copolymers and polyethylene oxide-polypropylene oxide random copolymers.3. The process according to claim 1 , wherein the polyether block copolymers comprising polyoxyethylene and polyoxypropylene moieties are triblock (PEO-PPO-PEO)-copolymers4. The process according to wherein the triblock (PEO-PPO-PEO)-copolymers are poloxamer 188 or poloxamer 407.5. The process according to claim 1 , wherein the solution of the polyether block copolymers treated by ultrafiltration is an aqueous solution or an organic solution or an aqueous-organic solvent mixture.6. The process according to claim 1 , wherein the solution of the polyether block copolymers treated by ultrafiltration is an aqueous solution.7. The process according to claim 1 , wherein a concentration of the polymer in the solution is 2-20% b.w.8. The process according to claim 1 , wherein a concentration of the polymer in the solution is 5-15% b.w.9. The process according to claim 1 , wherein the ultrafiltration is carried out using a membrane separating layer which is a ceramic or polymer or carbon material.10. The process according to claim 1 , wherein the ultrafiltration is carried out using a membrane separating layer ...

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03-02-2022 дата публикации

SIMULATED MOVING-BED TYPE CHROMATOGRAPHIC SEPARATION METHOD AND SIMULATED MOVING-BED TYPE CHROMATOGRAPHIC SEPARATION SYSTEM

Номер: US20220032208A1
Принадлежит: ORGANO CORPORATION

A simulated moving-bed type chromatographic separation method separating a weakly adsorptive component, a strongly adsorptive component, and an intermediately adsorptive component, with eluents by using a circulation system in which a plurality of unit packed columns packed with an adsorbent are connected in series and in an endless form via pipes in which a feed solution supply port F, two or more eluent supply ports D corresponding to the eluents, an extraction port A of a fraction containing the weakly adsorptive component, an extraction port B of a fraction containing the intermediately adsorptive component, and an extraction port C of a fraction containing the strongly adsorptive component are provided in the pipes of the circulation system, and positions of the ports F, A, B, and C are set to have a specified relationship. 1. A simulated moving-bed type chromatographic separation method comprising separating a weakly adsorptive component , a strongly adsorptive component , and an intermediately adsorptive component that has intermediate adsorptive property in relation to both components , with respect to an adsorbent , with two or more kinds of eluents by using a circulation system in which a plurality of unit packed columns packed with the adsorbent are connected in series and in an endless form via pipes , the weakly adsorptive component , the strongly adsorptive component , and the intermediately adsorptive component being contained in a feed solution , wherein a feed solution supply port F , two or more eluent supply ports D corresponding to the two or more kinds of eluents , an extraction port A of a weakly adsorptive fraction containing the weakly adsorptive component , an extraction port B of an intermediately adsorptive fraction containing the intermediately adsorptive component , and an extraction port C of a strongly adsorptive fraction containing the strongly adsorptive component are provided in the pipes of the circulation system , and positions of ...

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03-02-2022 дата публикации

Quantum Fluid Operation: Technology for Effective Mixing, Reacting, and Separating Fluids

Номер: US20220032246A1
Автор: Samid Gideon
Принадлежит:

A continuous chemical process is modified to allow parts thereto to be processed one quantum of matter at a time. This offers precision and efficiency beyond what is possible with the continuous mode. This Quantum Fluid Operation (QFO) is applied to basic unit operations: mixing, reacting, separating. 1. A system called “Quantum Fluid Operation” (QFO) for secluding a quantum of fluid in a continuous industrial flow , and treating this quantum as batch operation without affecting the control flow before and after the QFO; the QFO comprising:(i) input fluid capacity tanks, (A tanks),(ii) output fluid capacity tanks, (C tanks),(iii) a quantum fluid container, (B),(iv) operational implements,{'sub': 1', '2', 'f', 'a', 'c', 'a', 'in, 'the total flow of all the fluids a, a, . . . athrough the f A tanks is at a constant flow rate q, and the constant flow rate to the output fluid capacity tank is q=q; a quantum of fluid of measure Q is taken out of the f A tanks during time interval t, and is accumulated in the quantum fluid container, (B), which is big enough to contain the quantum fluid;'}{'sub': 'q', 'next the operational implements operate (treat) on the quantum Q contained in B for a period of time t;'}{'sub': 'out', 'claim-text': {'br': None, 'i': Q=q', 't', '+t', '+t', 'q', 't', '+t', '+t, 'sub': a', 'in', 'q', 'out', 'c', 'in', 'q', 'out, '*()=*()'}, 'next the quantum Q is pumped out of the container B to output fluid capacity tanks, (C), over a period of time t, the identified flow rates and timings comply with the following equation{'sub': 'q', 'and where the operational implements operating on the quantum of fluid Q, change the state of the quantum to a desired state, S=S(Q), and where the quantum treating time, t, is extended as needed to insure that the quantum of fluid, Q, leaves the quantum fluid container, (B) at the desired state, S(Q).'}2. The system in where the desired state of Q claim 1 , S(Q) is expressed as the desired temperature of Q claim 1 , (T) ...

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03-02-2022 дата публикации

Use Of A Hemocompatible Porous Polymer Bead Sorbent For Removal Of Endotoxemia-Inducing Molecules

Номер: US20220032267A1
Принадлежит:

The invention concerns biocompatible polymer systems comprising at least one polymer with a plurality of pores, said polymer comprising either polyol or zwitterionic groups designed to adsorb endotoxins and other inflammatory mediator molecules. The inventions are in the field of porous polymeric sorbents, also in the field of broadly reducing endotoxins in blood and blood products that can cause endotoxemia, additionally, in the field of broadly removing endotoxins by perfusion or hemoperfusion. 140-. (canceled)41. A method of adsorbing toxins and inflammatory mediators comprising contacting physiologic fluid with a polymer system comprising either polyol or zwitterionic functionality;said polymer system has the form of a solid support having a polymer coating comprising poly(diethylaminoethyl methacrylate), poly(dimethylaminoethyl methacrylate), poly(hydroxyethyl acrylate), poly(hydroxyethyl methacrylate), poly(hydroxypropyl acrylate), poly (hydroxypropyl methacrylate), poly(N-vinylpyrrolidone), poly(vinyl alcohol), salts of poly(acrylic acid), salts of poly(methacrylic) acid, or mixtures thereof.42. The method of wherein the said toxins and inflammatory mediators have a molecular weight of from less than 0.5 kDa to 1 claim 41 ,000 kDa.43. The method of wherein the said toxins and inflammatory mediators have a molecular weight of from less than 0.5 kDa to 60 kDa.44. The method of wherein the toxins and inflammatory mediators comprise one or more of cytokines claim 41 , pathogen-associated molecular pattern molecules (PAMPs) claim 41 , damage-associated molecular pattern molecules (DAMPs) claim 41 , superantigens claim 41 , monokines claim 41 , chemokines claim 41 , interferons claim 41 , proteases claim 41 , enzymes claim 41 , peptides including bradykinin claim 41 , soluble CD40 ligand claim 41 , bioactive lipids claim 41 , oxidized lipids claim 41 , cell-free hemoglobin claim 41 , cell-free myoglobin claim 41 , growth factors claim 41 , glycoproteins claim 41 , ...

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21-01-2016 дата публикации

Process for extraction and debitterizing sweet compounds from stevia plants

Номер: US20160015066A1
Принадлежит: Juvenex Inc

A method of extracting sweet compounds from stevia plant powder includes mixing stevia powder and deionized water to create a stevia powder slurry, filtering the slurry and adding it to an extraction column, adding an ethanol solution to create an elute, mixing the elute with activated charcoal and filtering the elute, removing the ethanol and water from the elute, and spraying the elute to produce the sweet compounds.

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21-01-2016 дата публикации

COMPOUND, 1-PALMITIN-2-LINOLEIN-3-OLEIN, AND PHARMACEUTICAL PREPARATION AND USE THEREOF

Номер: US20160015670A1
Автор: LI DAPENG
Принадлежит:

The invention relates to a compound, 1-palmitin-2-linolein-3-olein, having a structure of formula (I), which is prepared by a process comprising the preparation of seed oil from the seed powder; the preliminary separation of 1-palmitin-2-linolein-3-olein; and the double-separation of 1-palmitin-2-linolein-3-olein. The invention also relates to pharmaceutical preparations of 1-palmitin-2-linolein-3-olein, and the use of this compound and pharmaceutical preparations thereof in the treatment of tumors. 2. The compound of claim 1 , which is prepared by the process comprising steps of:{'i': 'Coix', '(1) preparation of seed oil;'}(2) preliminary separation of 1-palmitin-2-linolein-3-olein; and(3) double-separation of 1-palmitin-2-linolein-3-olein.3. The compound of claim 2 , wherein said step (1) comprises steps of:{'i': Coix', 'Coix', 'Coix', 'Coix', 'Coix, 'crushing seeds having a moisture content ≦10% into 10-300 mesh powder and extracting said powder using a supercritical fluid extraction to afford a crude seed oil; adding petroleum ether in an amount of 40-58% by weight of crude seed oil and caustically refining said crude seed oil with aqueous alkali, q.s.; after layering, adding neutral alumina and/or kaolin to the organic phase; filtering and sterilizing the filtrated oil via dry heat sterilization under vacuum at 150-180; then cooling and filtering the sterilized oil to give seed oil.'}4. The compound of claim 3 , wherein:{'sub': '2', 'said supercritical fluid extraction is the supercritical COextraction with an extraction temperature of 30-60, an extraction pressure of 19-24 Mpa, a separation temperature of 30-60, a separation pressure of 6-15 Mpa, a carbon dioxide flow rate of 10-5000 L/h, and an extraction time of 1-4 h;'}said aqueous alkali is a 0.5-3% KOH or NaOH aqueous solution; and{'i': Coix', 'Coix', 'Coix, 'said step of adding neutral alumina and/or kaolin includes adding 3-8% activated neutral alumina by weight of crude seed oil; filtering the mixture ...

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03-02-2022 дата публикации

Cooling loop with a supercritical fluid system using compressed refrigerant fluid flow with a positive joule thomson coefficient

Номер: US20220034555A1
Принадлежит: SUPERCRITICAL FLUID TECHNOLOGIES Inc

Provided is a chiller and system that may be utilized in a supercritical fluid chromatography method, wherein a non-polar solvent may replace a portion or all of a polar solvent for the purpose of separating or extracting desired sample molecules from a combined sample/solvent stream. The system may reduce the amount of polar solvent necessary for chromatographic separation and/or extraction of desired samples. The system may incorporate a supercritical fluid chiller, a supercritical fluid pressure-equalizing vessel and a supercritical fluid cyclonic separator. The supercritical fluid chiller allows for efficient and consistent pumping of liquid-phase gases employing off-the-shelf HPLC pumps. The pressure equalizing vessel allows the use of off-the-shelf HPLC column cartridges. The system may further incorporate the use of one or more disposable cartridges containing silica gel or other suitable medium. The system may also utilize an open loop cooling circuit using fluids with a positive Joule-Thomson coefficient.

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21-01-2021 дата публикации

METHODS AND SYSTEMS FOR PRODUCING LOW SUGAR BEVERAGES

Номер: US20210015127A1
Принадлежит:

Methods and systems are disclosed for selectively removing naturally-occurring sugars in beverages in an effective, affordable and scalable manner. 1. A method of lowering the sugar content of a fruit juice , the method comprising:(a) separating at least part of solid components from the juice; and(b) contacting a first adsorbent with the juice, the first adsorbent being active so as to have a higher relative selectivity for disaccharides than for monosaccharides and organic acids, wherein the first adsorbent comprises a zeolite, to treat the juice and obtain a treated juice having at least 30% less sugar than the untreated form of the juice and a ratio of disaccharides to total sugars below at least 30 percent.2. The method of claim 1 , further comprising the steps of:hydrolyzing the disaccharides bound to the first adsorbent into monosaccharides after the juice has contacted the first adsorbent; wherein the hydrolyzing is performed by heating the first absorbent;washing the first adsorbent with a solution to remove the hydrolyzed monosaccharides, wherein the solution comprises at least one of: water, water in a mixture with at least one of fructose, glucose and galactose, and a solution of at least one of fructose, glucose and galactose; andregulating the acidity of the treated juice to have a pH of at least about 4.3. The method of claim 1 , wherein a Brix/acidity ratio of the treated juice is decreased to be less than about 20% claim 1 , when compared to the untreated form of the juice.45.-. (canceled)6. The method of claim 1 , wherein the first adsorbent is in a column.78.-. (canceled)9. The method of claim 1 , wherein the zeolite is selected from zeolites having a Si/Al molar ratio of at least about 10:1 and up to about 30:1.10. The method of claim 1 , wherein the zeolite comprises at least one of: Y Zeolite H claim 1 , and Y Zeolite Ca.1114.-. (canceled)15. The method of claim 1 , wherein the first adsorbent is associated with a carrier claim 1 , wherein the ...

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21-01-2016 дата публикации

Simulated Moving Bed Chromatographic Separation Process

Номер: US20160016877A1
Принадлежит:

The present invention provides a chromatographic separation process for recovering a polyunsaturated fatty acid (PUFA) product, from a feed mixture, which process comprises introducing the feed mixture to a simulated or actual moving bed chromatography apparatus having a plurality of linked chromatography columns containing, as eluent, an aqueous alcohol, wherein the apparatus has a plurality of zones comprising at least a first zone and a second zone, each zone having an extract stream and a raffinate stream from which liquid can be collected from said plurality of linked chromatography columns, and wherein (a) a raffinate stream containing the PUFA product together with more polar components is collected from a column in the first zone and introduced to a nonadjacent column in the second zone, and/or (b) an extract stream containing the PUFA product together with less polar components is collected from a column in the second zone and introduced to a nonadjacent column in the first zone, said PUFA product being separated from different components of the feed mixture in each zone. 1. A composition comprising a PUFA product , wherein the PUFA product is EPA present in an amount greater than 93 wt % , wherein the total content of ω-6 polyunsaturated fatty acids is up to 0.40 wt % , and wherein the total content of eicosatetraenoic acid is up to 1 wt %.2. The composition according to claim 1 , wherein the total content of eicosatetraenoic acid is 0.05 wt % or greater.3. The composition according to claim 1 , wherein the composition (a) comprises greater than 96.5 wt % EPA claim 1 , up to 1 wt % DHA claim 1 , up to 1 wt % α-linolenic acid claim 1 , up to 1 wt % stearidonic acid claim 1 , up to 1 wt % eicosatetraenoic acid claim 1 , up to 1 wt % docosapentaenoic acid claim 1 , and up to 0.25 wt % arachidonic acid; or(b) comprises greater than 96.5 wt % EPA, up to 0.2 wt % DHA, up to 0.3 wt % α-linolenic acid, up to 0.4 wt % stearidonic acid, up to 0.5 wt % ...

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17-01-2019 дата публикации

Planetary Countercurrent Chromatography Centrifuge and Mixer-Settler Rotor

Номер: US20190015761A1
Принадлежит:

A mixer-settler countercurrent chromatography centrifuge and rotor of increased capacity is described. 2. The centrifuge of wherein said shafts comprise a sealed bearing.3. The centrifuge of wherein said shafts comprise a terminal flared portion.4. The centrifuge of claim 1 , comprising at least six disks.5. The centrifuge of claim 1 , comprising at least eight discs.6. The centrifuge of wherein said discs comprise a raised lip about an outer edge of said disk.7. The centrifuge of wherein said discs comprise a raised lip about an inner edge of said disk.8. The centrifuge of wherein said gaskets seat within said raised lip.9. The centrifuge of wherein said gaskets seat within said raised lip.10. The centrifuge of wherein said rotor comprises ribbed endplates.11. The centrifuge of wherein said rotor comprises a tubing protector.12. The centrifuge of wherein said tubing protector comprises a sleeve.13. The centrifuge of wherein said sleeve is corrugated.14. The centrifuge of wherein said tubing protector comprises a foam claim 11 , a sponge or a hydrogel. Portions of the research described herein were supported in part by NIH grant no. R43AT008296-01 to CC Biotech, Rockville, Md.Carbon nanotubes (CNT), carbon-carbon extended polymers with fused sp2 orbitals, have aromatic properties [1,2]. Sheets are known as graphenes and can form tubes, nanotubes. Tubular forms are of various diameter from about 1 rim to a few hundred urn and have lengths of about 500 nm to several thousands of nanometers. Nanotubes are single-walled or multi-walled, having anisotropic structures. Nanotubes exhibit conductive or semiconductor properties, and are chiral. The hexagonal array of the atoms is in a left-handed or a right-handed spiral pattern. Dimensions of patterns of a hexagonal honeycomb lattice are described by vectors, m and n, where nanotubes of certain values of m and n being semiconducting. A, ‘zig-zag,’ pattern where m=0 and an, ‘arm chair,’ pattern where n=m (metallic) are non- ...

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17-01-2019 дата публикации

Multi-Vessel Filtration System for Hazardous or Radioactive Waste Water

Номер: US20190015762A1
Принадлежит:

Surface or submersible sluiceable systems are disclosed for use in removing hazardous contaminants or radioactive isotopes from a fluid stream, such as a fluid stream from the primary coolant loop or secondary loop of a nuclear reactor system, or a fluid stream from a spent-fuel pool or pond or hazardous or radioactive contaminants in ground water. Generally, this surface or submersible sluiceable system is adapted to be utilized in a surface skid or submersed in the fluid stream, and additionally the vessels are adapted to be sluiced and reused after use, resulting in a potentially stabilized, non-leaching final waste product with a substantially reduced volume for storage or disposal. The system can be utilized with standard ion exchange beads or preferably with inorganic granular media. 1. A surface or submersible sluiceable lead-lag system to remove selected hazardous contaminants or radioactive isotopes from fluid waste materials , the system comprising: a vessel body having an interior volume,', 'a fill head having a plurality of ports giving access to the interior of said vessel body, including a fluid waste material input port, a treated fluid waste material output port, a sluice-in port to facilitate delivery of media to the interior of said vessel body, a sluice-out port to facilitate removal of spent media from the interior of said vessel body, and a sluice water input port,', 'internal media containment screens within the vessel body,', 'an internal waste fluid line to deliver fluid waste materials from said fluid waste material input port to a location within the interior of the vessel body, said location being placed such that fluid waste materials exiting the internal waste fluid line pass through said media before exiting the interior of the vessel body through said treated fluid waste material output port,', 'a sluice-in tube to deliver media into the vessel during filling, and', 'a sluice-out tube to remove media from the vessel, said sluice-out ...

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15-01-2015 дата публикации

Fc-receptor based affinity chromatography

Номер: US20150018241A1
Принадлежит: Hoffmann La Roche Inc

Herein is reported the use of an immobilized non-covalent complex of a neonatal Fc receptor (FcRn) and beta-2-microglobulin (b2m) as affinity chromatography ligand in general and, for example, for the determination of the in vivo half-live of an antibody by determining the ratio of the retention times of the antibody and a reference antibody.

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18-01-2018 дата публикации

ANTIBODIES COMPRISING SEQUENCES FROM CAMELIDAE TO HIGHLY CONSERVED TARGETS

Номер: US20180016351A1
Принадлежит:

The present invention is concerned with antigen binding polypeptides, and in particular conventional antibodies derived from camelid species, that specifically bind to target antigens that are either self-antigens or highly conserved antigens. The present invention also relates to anti-idiotype antigen binding peptides which bind to a target antigen comprising the variable region of an antibody obtained from a species within the family Camelidae. 117-. (canceled)18. A method for preparing a recombinant antigen binding polypeptide that specifically binds to a target antigen , said antigen binding polypeptide comprising a VH domain and a VL domain , wherein at least one hypervariable loop or complementarity determining region (CDR) in the VH domain or the VL domain is obtained from a species in the family Camelidae , wherein the target antigen is a polypeptide antigen that exhibits at least 90% amino acid sequence identity with a protein from said species in the family Camelidae over a sequence comparison window , said method comprising the step of:(a) immunizing a species in the family Camelidae with the target antigen, thereby raising a conventional antibody to said target antigen.19. (canceled)20. (canceled)21. The method of claim 18 , wherein the species in the family Camelidae is selected from the group consisting of camel claim 18 , llama claim 18 , dromedary claim 18 , vicuna claim 18 , guanaco claim 18 , and alpaca.2233-. (canceled)34. The method of claim 18 , wherein the sequence comparison window includes at least the epitope for the antigen binding polypeptide.35. The method of claim 18 , wherein the sequence comparison window comprises at least one domain of the target antigen claim 18 , including the epitope for the antigen binding polypeptide.36. The method of claim 18 , wherein the sequence comparison window includes the full length of the target antigen.37. The method of claim 18 , further comprising the steps of:(b) isolating Camelidae nucleic acid ...

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18-01-2018 дата публикации

METHOD FOR TREATING LIGNOCELLULOSIC MATERIALS

Номер: US20180016649A1
Принадлежит:

A method of generating a refined sugar stream that comprises xylose from a biomass hydrolysis solution, including contacting a biomass hydrolysis solution that includes a population of mixed sugars comprising xylose, an acid, and impurities, with a thermally-phase separable solvent such as a glycol solvent to form an extraction mixture; and separating from said extraction mixture a first stream including the thermally-phase separable solvent, acid, and impurities and a second, refined sugar stream that comprises xylose. The thermally-phase separable solvent is an ethylene glycol or a propylene glycol ether, such as 2-butoxyethanol or 1-propoxy-propanol or any combination thereof. 1. A method of generating a refined a sugar stream that comprises xylose from a biomass hydrolysis solution , comprising:(i) contacting a biomass hydrolysis solution that comprises a population of mixed sugars comprising xylose, an acid, and impurities, with a thermally-phase separable solvent to form an extraction mixture; and(ii) separating from said extraction mixture a first stream comprising the thermally-phase separable solvent, acid, and impurities and a second, refined sugar stream that comprises xylose.2. The method of claim 1 , further comprising claim 1 , contacting a stream from said biomass hydrolysis solution claim 1 , which comprises said population of mixed sugars comprising xylose with a strong acid cation exchange resin prior to step (i).3. The method of claim 2 , further comprising claim 2 , contacting a stream from said biomass hydrolysis solution claim 2 , which comprises said population of mixed sugars comprising xylose with a weak base anion exchange resin after said stream is contacted with said strong acid cation exchange resin and prior to step (i).4. The method of claim 1 , further comprising heating said extraction mixture to a temperature of 30-100° C.5. The method of claim 1 , further comprising separating said second claim 1 , refined sugar stream that ...

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17-01-2019 дата публикации

MULTI-STEP SEPARATION PROCESS

Номер: US20190016663A1
Принадлежит:

The present invention provides a chromatographic separation process for recovering a polyunsaturated fatty acid (PUFA) product from a feed mixture, which comprises: (a) purifying the feed mixture in a first chromatographic separation step using an eluent a mixture of water and a first organic solvent, to obtain an intermediate product; and (b) purifying the intermediate product in a second chromatographic separation step using as eluent a mixture of water and a second organic solvent, to obtain the PUFA product, wherein the second organic solvent is different from the first organic solvent and has a polarity index which differs from the polarity index of the first organic solvent by between 0.1 and 2.0, wherein the PUFA product is other than alpha-linolenic acid (ALA), gamma-linolenic acid (GLA), linoleic acid, an ALA mono- di- or triglyceride, a GLA mono- di- or triglyceride, a linoleic acid mono- di- or triglyceride, an ALA C-Calkyl ester, a GLA C-Calkyl ester or a linoleic acid C-Calkyl ester or a mixture thereof. 2. The process according to claim 1 , wherein the first chromatographic separation step consists of two chromatographic separations claim 1 , wherein each separation uses as eluent a mixture of water and the first organic solvent.3. The process according to claim 1 , wherein the second chromatographic separation step consists of two chromatographic separations claim 1 , wherein each separation uses as eluent a mixture of water and the second organic solvent.4. The process according to claim 1 , wherein the first and second organic solvents are chosen from alcohols claim 1 , ethers claim 1 , esters claim 1 , ketones and nitriles.5. The process according to claim 4 , wherein the ketone is acetone claim 4 , methylethylketone or methyl isobutyl ketone (MIBK) claim 4 , preferably acetone.6. The process according to claim 1 , wherein one of the first and second organic solvents is methanol.7. The process according to claim 1 , wherein the second organic ...

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21-01-2016 дата публикации

MATCHING THERMALLY MODULATED VARIABLE RESTRICTORS TO CHROMATOGRAPHY SEPARATION COLUMNS

Номер: US20160018367A1
Принадлежит:

Thermally modulated variable restrictors used in chromatography systems enable independent control of system pressure and linear velocity of a compressible mobile phase passing through a chromatography column. A method for configuring a chromatography system with independent control of system pressure and mass flow rate of a compressible mobile phase includes determining a type of chromatography separation column to be used in the chromatography system, matching a thermally modulated variable restrictor to the type of chromatography separation column for use together during operation of the chromatography system, and bundling the chromatography column with its matching thermally modulated variable restrictor for distribution as a single package.

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17-04-2014 дата публикации

Devices and methods for determination of bioavailability of pollutants

Номер: US20140102182A1

Contaminant mass collection in saturated sedimentary environments for bioavailability determination. A casing includes a screen between the environment that is subject to sampling, such as a saturated sediment and the device itself. The casing includes a water intake zone, a pump, and sorptive media. The water intake zone, the pump, the screen and the sorptive media, are all operably linked in sequence. The screened casing is secured to form an in situ device; the screen is in fluid communication with the water intake zone and excludes endemic sediments and aquatic life. The in situ device is deployed in the saturated sedimentary environment. The pump operates to concentrate analytes from the selected environment in the sorptive media, where the concentrated analytes include the analyte mass of time-weighted fluid samples.

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03-02-2022 дата публикации

Method for producing lead-212 from an aqueous solution comprising thorium-228 and daughters thereof

Номер: US20220037046A1
Автор: Julien Torgue, Remy Dureau
Принадлежит: Orano Med SAS

A method for producing lead-212 of very high radiological purity from an aqueous solution comprising thorium-228 and daughters thereof. Manufacture of radiopharmaceuticals based on lead-212, which are useful in nuclear medicine and, in particular, in targeted alpha radiation therapy for the treatment of cancers.

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