High pressure homogenizer and manufacturing method for Graphene using the same

aparelho multiprocessador microfluídico e seu uso

Rapid production of droplets

The present invention generally relates to the production of fluidic droplets. Certain aspects of the invention are generally directed to systems and methods for creating droplets by flowing a fluid from a first channel to a second channel through a plurality of side channels. The fluid exiting the side channels into the second channel may form a plurality of droplets, and in some embodiments, at very high droplet production rates. In addition, in some aspects, double or higher-order multiple emulsions may also be formed. In some embodiments, this may be achieved by forming multiple emulsions through a direct, synchronized production method and/or through the formation of a single emulsion that is collected and re-injected into a second microfluidic device to form double emulsions.

Process for oxidising a substrate

dispositivo microfluídico, método, sistema e kit

Liquid mixing apparatus

During the use of a liquid mixing apparatus, a liquid container can be installed onto an adapter. The liquid container drives a lifting assembly to allow a piercing assembly to pierce through the adapter. Once a fluid flows into a flowing passage portion via a fluid inlet, a portion of the fluid is pressurized via a branch passage portion with easy control and further flows into the liquid container to mix with a fragrance material inside the liquid container. The mixed fragrant liquid then flows out of a fluid outlet from the piercing assembly via the return passage portion and the flowing passage portion. With the pressurization design of the branch passage portion, the apparatus allows the increase of dimension to increase the flow rate while maintaining the pressurized liquid, which is convenient to use and facilitated for the control of the mixture amount with greater variation range.

Microstructure flow mixing devices

Microstructure flow mixing devices are disclosed herein. An example device a first panel, a first plurality of raised features extending from a first surface of the first panel, a second plurality of raised features extending from the first surface of the first panel and a plurality of divider microstructures extending from the first surface of the first panel in line with and in between the first plurality of raised features and the second plurality of raised features. At least a portion of adjacent divider microstructures are spaced apart to form feed pathways or cross channels.

MICROFLUIDIC DEVICE FOR THE GENERATION OF COMBINATORIAL SAMPLES

The present disclosure relates to a microfluidic device and a method allowing the generating and screening of combinatorial samples. A microfluidic device for producing droplets of at least one sample into an immiscible phase is provided, the device comprising a drop- let maker connecting an immiscible phase channel and a sample channel having at least one sample inlet connected to at least one sample inlet channel injecting the at least one sample into the sample channel, wherein the injection of the at least one sample is con- trolled by at least one sample valve, so that the at least one sample flows either towards a sample waste outlet or into the at least one sample inlet channel, wherein different sample inlet channel of the at least one sample inlet channel have the same hydrodynamic re- sistance resulting from the length, height and width of each sample inlet channel upstream of the droplet maker.

DISPOSITIF ET PROCEDE POUR REALISER UNE EMULSION EN CONTINU DE DEUX LIQUIDES IMMISCIBLES

La présente invention a pour objet un dispositif (1) pour réaliser une émulsion en continu de deux fluides immiscibles, muni d'un premier microsystème (2) comprenant au moins deux micro-canaux (23, 24) d'admission de chaque fluide sections respectives S1 et S2 différentes, se faisant face le long d'un axe central A d'admission et présentant un ex-centrage, au moins deux micro-canaux (25, 26) de sortie dudit dispositif (1) de l'émulsion une fois formée, et une zone de croisement (27) des micro-canaux d'admission et de sortie, apte à générer une interface entre les fluides et constituer une pré-émulsion circulant dans les micro-canaux de sortie jusqu'à l'achèvement de l'émulsion. Selon l'invention, le dispositif (1) comprend en outre au moins une singularité (31, 32, 33, 34, 35, 36) apte à déstabiliser les interfaces entre les fluides dans la pré-émulsion. La présente invention a également pour objet un procédé pour réaliser une émulsion en continu de deux liquides immiscibles mettant en ...

DEVICE AND PROCESS FOR MAKING AN EMULSION OF TWO IMMISCIBLE LIQUIDS CONTINUOUSLY

La présente invention a pour objet un dispositif (1) pour réaliser une émulsion en continu de deux fluides immiscibles, muni d'un premier microsystème (2) comprenant au moins deux micro-canaux (23, 24) d'admission de chaque fluide sections respectives S1 et S2 différentes, se faisant face le long d'un axe central A d'admission et présentant un ex-centrage, au moins deux micro-canaux (25, 26) de sortie dudit dispositif (1) de l'émulsion une fois formée, et une zone de croisement (27) des micro-canaux d'admission et de sortie, apte à générer une interface entre les fluides et constituer une pré-émulsion circulant dans les micro-canaux de sortie jusqu'à l'achèvement de l'émulsion. Selon l'invention, le dispositif (1) comprend en outre au moins une singularité (31, 32, 33, 34, 35, 36) apte à déstabiliser les interfaces entre les fluides dans la pré-émulsion. La présente invention a également pour objet un procédé pour réaliser une émulsion en continu de deux liquides immiscibles mettant en ...

Microfluidic devices, and methods of making and using the same

The present disclosure provides methods and systems for assaying a sample. A microfluidic device to perform an assay of a sample (e.g., biological sample) is described having a sample application site, a porous component and a flow channel. The porous component provides for uniform dissolution of a reagent and mixing of the sample and reagent without filtering the sample.

Microfluidic devices, and methods of making and using the same

The present disclosure provides methods and systems for assaying a sample. A microfluidic device to perform an assay of a sample (e.g., biological sample) is described having a sample application site, a porous component and a flow channel. The porous component provides for uniform dissolution of a reagent and mixing of the sample and reagent without filtering the sample.

MICROFLUIDIC MODULE FOR CO-ENCAPSULATION IN DROPLETS

Microfluidic module (100) for co-encapsulation in droplets of two populations of particles, comprising a first (1) and a second (2) modules for sorting said two populations, said modules (1, 2) having their first outlets (12, 22) comprising first obstructive valves (121, 221) configured to at least partially obstruct said first outlets (12, 22), said first outlets (12, 22) being fluidly connected to: - a fusion module (3) comprising: a fusion module means (30) for merging at least one droplet from the first droplet population and at least one droplet from the second droplet population into a merged droplet comprising said two population of particles, - a control unit (4) for controlling: said first obstructive valves (121, 221) from information originating from a first (19) and second (29) module detection portion located upstream of said first outlets (12, 22).

Tubular flow type reactor

APPARATUS AND METHODS FOR MAKING RECOMBINANT PROTEIN-STABILIZED MONODISPERSE MICROBUBBLES

A microfluidic device for generating mi-crobubbles includes a substrate and a microfluidic channel embedded in the substrate. The microfluidic channel includes a plurality of fluid inlets, at least one bubble formation outlet having a nozzle with an adjustable diameter, and a flow focusing junction in fluid communication with the plurality of fluid inlets and the bubble formation outlet. A method for mass producing monodisperse microbubbles with a microfluidic device includes supplying a flow of dispersed phase fluid into a first fluid inlet of a microfluidic channel, supplying a flow of continuous phase fluid into a second fluid inlet of the microfluidic channel, and adjusting a diameter of a nozzle to obtain a plurality of monodisperse microbubbles having a specified diameter.

Microfluidic devices, and methods of making and using the same

The present disclosure provides methods and systems for assaying a sample. A microfluidic device to perform an assay of a sample (e.g., biological sample) is described having a sample application site, a porous component and a flow channel. The porous component provides for uniform dissolution of a reagent and mixing of the sample and reagent without filtering the sample.

Controllable-proportioning microflow mixer taking magnetic liquid as medium

APPARATUS FOR MANUFACTURING COSMETIC USING INSTANTANEOUS EMULSIFICATION

The present invention relates to an apparatus for manufacturing cosmetic using instantaneous emulsification. Provided according to an aspect of the invention may be an apparatus for manufacturing cosmetic using instantaneous emulsification, which includes a housing which forms an outer appearance; an internal phase container which is replaceably coupled to the housing, and which stores internal phase fluid; an external phase container which is replaceably coupled to the housing, and which stores external phase fluid; a channel unit which generates emulsion by mixing the internal phase fluid provided from the internal phase container and the external phase fluid provided from the external phase container; and an operative unit which provides external force required to form and discharge emulsion at the channel unit by manipulation of a user. 1. An apparatus for manufacturing cosmetic using instantaneous emulsification , the apparatus comprising:a housing which forms an outer appearance;an internal phase container which is replaceably coupled to the housing, and which stores internal phase fluid;an external phase container which is replaceably coupled to the housing, and which stores external phase fluid;a channel unit which generates emulsion by mixing the internal phase fluid provided from the internal phase container and the external phase fluid provided from the external phase container; andan operative unit which provides external force required to form and discharge emulsion at the channel unit by manipulation of a user,wherein the internal phase container and the external phase container have a pumping part which is operated by action of the operative unit, andwherein the operative unit presses the pumping part of the internal phase container and the pumping part of the external phase container at the same time by external force to discharge the internal phase fluid stored in the internal phase container and the external phase fluid stored in the external phase ...

Fluid mixer

Microfabricated cellular traps based on three-dimensional micro-scale flow geometries

An improved microfluidic device and method of using the microfluidic device to measure natural motile response of a living moiety to a chemotactic agent. The invention comprises integrating microfluidic containment trenches within the microfluidic devices for cellular trapping, manipulation and real-time analysis of biological phenomena, including chemotaxis. The invention captures the design methodology for these traps, as well as the fabrication means and the associated external control mechanism that allows rapid, reversible and fully controlled changes in the chemical environment to which trapped cells are exposed.

DEVICE AND METHOD FOR CARRYING OUT A CONTINUOUS EMULSION OF TWO IMMISCIBLE LIQUIDS

Some embodiments relate to a device for performing continuous emulsion of two immiscible fluids. The device includes: a first microsystem including at least two micro-channels for intake of each fluid, of different respective cross sections S and S which are offset and face each other along a central intake axis A; at least two micro-channels for output of the emulsion from the device once the emulsion is formed; and an area where the intake and output micro-channels intersect, the area being capable of generating an interface between the fluids and forming a pre-emulsion flowing in the output micro-channels until the emulsion is complete. The device also includes at least one singularity capable of destabilizing the interfaces between the fluids in the pre-emulsion. 1. A device for performing a continuous emulsion of two immiscible fluids , comprising: [{'b': 1', '2', '1, 'at least two micro-channels for the intake of each fluid into the device, the micro-channels, with respective sections S and S different from S, facing each other along a central intake axis A and having an offset, linked to their difference in section,'}, 'at least two micro-channels for the output from the device of the emulsion once formed,', 'an intersection area wherein the intake and output micro-channels intersect, the intersection area being able to generate an interface between the fluids and as such forming a pre-emulsion intended to flow in the output micro-channels until the completion of the forming of the emulsion, and, 'at least one first microsystem that includesat least one singularity capable of destabilizing the interfaces between the fluids in the pre-emulsion.2. The device according to claim 1 , wherein the output micro-channels are arranged claim 1 , in the microsystem symmetrically claim 1 , with respect to the central intake axis (A).3. The device as claimed in claim 1 , wherein the singularity is a bend formed in each output micro-channel of the microsystem.4. The device ...

DEVICE AND METHOD FOR CARRYING OUT CONTINUOUS EMULSION OF TWO IMMISCIBLE LIQUIDS

Apparatus and method for the rapid production of droplets

Apparatus and methods for making recombinant protein-stabilized monodisperse microbubbles

A microfluidic device for generating microbubbles includes a substrate and a microfluidic channel embedded in the substrate. The microfluidic channel includes a plurality of fluid inlets, at least one bubble formation outlet having a nozzle with an adjustable diameter, and a flow focusing junction in fluid communication with the plurality of fluid inlets and the bubble formation outlet. A method for mass producing monodisperse microbubbles with a microfluidic device includes supplying a flow of dispersed phase fluid into a first fluid inlet of a microfluidic channel, supplying a flow of continuous phase fluid into a second fluid inlet of the microfluidic channel, and adjusting a diameter of a nozzle to obtain a plurality of monodisperse microbubbles having a specified diameter.

TUBULAR FLOW TYPE REACTOR

MICROFABRICATED CELLULAR TRAPS BASED ON THREE-DIMENSIONAL MICRO-SCALE FLOW GEOMETRIES

An improved microfluidic device and method of using the microfluidic device to measure natural motile response of a living moiety to a chemotactic agent. The invention comprises integrating microfluidic containment trenches within the microfluidic devices for cellular trapping, manipulation and real-time analysis of biological phenomena, including chemotaxis. The invention captures the design methodology for these traps, as well as the fabrication means and the associated external control mechanism that allows rapid, reversible and fully controlled changes in the chemical environment to which trapped cells are exposed.

MICROFABRICATED CELLULAR TRAPS BASED ON THREE-DIMENSIONAL MICRO-SCALE FLOW GEOMETRIES

An improved microfluidic device and method of using the microfluidic device to measure natural motile response of a living moiety to a chemotactic agent. The invention comprises integrating microfluidic containment trenches within the microfluidic devices for cellular trapping, manipulation and real-time analysis of biological phenomena, including chemotaxis. The invention captures the design methodology for these traps, as well as the fabrication means and the associated external control mechanism that allows rapid, reversible and fully controlled changes in the chemical environment to which trapped cells are exposed.

High pressure homogenizer and method for manufacturing graphene using the same

The present invention relates to a high pressure homogenizer and a method for manufacturing graphene using the same, and according to one aspect of the present invention, there is provided a high pressure homogenizer comprising a channel module which comprises a microchannel through which an object for homogenization passes, wherein the channel module comprises at least one baffle disposed so as to partition the microchannel into a plurality of spaces and the baffle is provided so as to partition the microchannel into two spaces along the width direction or the height direction.

dispositivo microfluídico, método, sistema e kit

TUBULAR FLOW TYPE REACTOR

A tubular flow type reactor comprising: (1) inflow channels for respective inflow of at least two kinds of fluids to be used in a reaction, (2) an outer tube having a lumen that is capable of merging the fluids and of distributing the merged fluids for the reaction, (3) an outflow channel for outflow of a reaction product from the outer tube, and (4) an inner tube disposed in the lumen of the outer tube, wherein an annular flow channel is formed between the outer tube and the inner tube, and the inflow channels are connected to the outer tube along the direction tangential to the internal circumference of the outer tube in such a manner that the lumens of the inflow channels communicate with the lumen of the outer tube, i.e., the annular flow channel.

PROCESS FOR OXIDISING A SUBSTRATE

A process for oxidising a substrate selected from hydroxymethylfurfural (HMF), diformylfuran (DFF), hydroxymethylfurancarboxylic acid (HMFCA) and formylfurancarboxylic acid (FFCA). Said process comprises mixing said substrate with catalase, one or more further enzymes and hydrogen peroxide to form a reaction mixture. Said one or more further enzymes have the ability to catalyse oxidation of said substrate. Said hydrogen peroxide is provided at a total molar ratio of at least about 0.1:1 hydrogen peroxide to substrate.

MODULAR MICROCHANNEL THERMAL SOLUTIONS FOR INTEGRATED CIRCUIT DEVICES

A microfluidic device having a channel within a first material to thermally couple with an IC die. The channel defines an initial fluid path between a fluid inlet port and a fluid outlet port. A second material is within a portion of the channel. The second material supplements the first material to modify the initial fluid path into a final fluid path between the fluid inlet port and the fluid outlet port. The second material may have a different composition and/or microstructure than the first material.

Microfluidic devices, and methods of making and using the same

Microchannel device and method pertaining thereto

A microchannel device includes: an upstream channel portion configured to allow an upstream liquid plug and a gas to flow therethrough; a downstream channel portion configured to allow a downstream liquid plug and a gas to flow therethrough; a liquid holding portion provided between a downstream end portion of the upstream channel portion and an upstream end portion of the downstream channel portion, the liquid holding portion being configured to hold a main liquid plug therein; and a gas bypass channel portion provided so as to bypass the liquid holding portion from the downstream end portion of the upstream channel portion to the upstream end portion of the downstream channel portion, the gas bypass channel portion being configured to allow the gas to flow therethrough in a state in which the liquid holding portion holds the main liquid plug.

Microfluidic mixing using channel width variation for enhanced fluid mixing

A fluid mixing method using a micromixing apparatus which includes a mixing microchannel having a channel length and a continuously variable channel width defined by a first sidewall surface and an opposing second sidewall surface. The channel width varies from a minimum channel width h to a maximum channel width H in a ratio of H:h≧1.1:1.0. A first inlet injects a first fluid and a second inlet a second fluid into the mixing microchannel which both flow in a flow direction in the mixing microchannel along the channel length. The first sidewall surface includes first curved surface portions and the second sidewall surface includes second curved surface portions. The first and second curved surface portions are non-overlapping to provide the variable channel width. The flow velocity profile is passively varied and exclusively controlled by the continuously variable channel width.

Microreaction chip, microfluidic mixing method and preparation method thereof

3-D type tightening micro mixer

Microstructure mixer for high-viscosity fluid mixing

Multi-layer micro/nanofluid devices with bio-nanovalves

A user-friendly multi-layer micro/nanofluidic flow device and micro/nano fabrication process are provided for numerous uses. The multi-layer micro/nanofluidic flow device can comprise: a substrate, such as indium tin oxide coated glass (ITO glass); a conductive layer of ferroelectric material, preferably comprising a PZT layer of lead zirconate titanate (PZT) positioned on the substrate; electrodes connected to the conductive layer; a nanofluidics layer positioned on the conductive layer and defining nanochannels; a microfluidics layer positioned upon the nanofluidics layer and defining microchannels; and biomolecular nanovalves providing bio-nanovalves which are moveable from a closed position to an open position to control fluid flow at a nanoscale.

Multi-channel micro-reaction chip, micro-fluid mixing method and preparation method thereof

Apparatus for manufacturing cosmetic using instantaneous emulsification

The present invention relates to an apparatus for manufacturing cosmetic using instantaneous emulsification. Provided according to an aspect of the invention may be an apparatus for manufacturing cosmetic using instantaneous emulsification, which includes a housing which forms an outer appearance; an internal phase container which is replaceably coupled to the housing, and which stores internal phase fluid; an external phase container which is replaceably coupled to the housing, and which stores external phase fluid; a channel unit which generates emulsion by mixing the internal phase fluid provided from the internal phase container and the external phase fluid provided from the external phase container; and an operative unit which provides external force required to form and discharge emulsion at the channel unit by manipulation of a user.

MICROFLUIDIC DEVICES, AND METHODS OF MAKING AND USING THE SAME

The present disclosure provides methods and systems for assaying a sample. A microfluidic device to perform an assay of a sample (e.g., biological sample) is described having a sample application site, a porous component and a flow channel. The porous component provides for uniform dissolution of a reagent and mixing of the sample and reagent without filtering the sample.

TUBULAR FLOW TYPE REACTOR

A tubular flow type reactor comprising: (1) inflow channels for respective inflow of at least two kinds of fluids to be used in a reaction, (2) an outer tube having a lumen that is capable of merging the fluids and of distributing the merged fluids for the reaction, (3) an outflow channel for outflow of a reaction product from the outer tube, and (4) an inner tube disposed in the lumen of the outer tube, wherein an annular flow channel is formed between the outer tube and the inner tube, and the inflow channels are connected to the outer tube along the direction tangential to the internal circumference of the outer tube in such a manner that the lumens of the inflow channels communicate with the lumen of the outer tube, i.e., the annular flow channel.

MICROFLUIDIC MODULE FOR CO-ENCAPSULATION IN DROPLETS

A microfluidic module for co-encapsulation in droplets of two populations of particles may include first and second modules for sorting the two populations. The modules may have their first outlets including first obstructive valves configured to at least partially obstruct the first outlets. The first outlets may be fluidly connected to a fusion module, including a fusion module means for merging at least one droplet from the first droplet population and at least one droplet from the second droplet population into a merged droplet comprising the two population of particles, and a control unit for controlling the first obstructive valves from information originating from a first and second module detection portion located upstream of the first outlets. 1. Microfluidic module for co-encapsulation in droplets of two populations of particles , comprising: a first module inlet for directing a first droplet population and comprising a first module detection portion for detecting said first droplet population inside said first module inlet,', 'a first outlet of said first module in fluid communication with said first module inlet, allowing directing said first droplet population along a first outlet trajectory of said first module and comprising a first obstructive valve of said first module configured to at least partially obstruct said first outlet of said first module,', 'a first module waste outlet in fluid communication with said first module inlet and with said first outlet of said first module, allowing directing a first population droplet along a first waste trajectory of said first module;, 'a first module comprising a second module inlet for directing said second droplet population and comprising a second module detection portion for detecting said second droplet population inside said second module inlet,', 'a first outlet of said second module in fluid communication with said second module inlet, allowing directing said second droplet population along a first ...

MULTI-LAYER MICRO/NANOFLUID DEVICES WITH BIO-NANOVALVES

A user-friendly multi-layer micro/nanofluidic flow device and micro/nano fabrication process are provided for numerous uses. The multi-layer micro/nanofluidic flow device can comprise: a substrate, such as indium tin oxide coated glass (ITO glass); a conductive layer of ferroelectric material, preferably comprising a PZT layer of lead zirconate titanate (PZT) positioned on the substrate; electrodes connected to the conductive layer; a nanofluidics layer positioned on the conductive layer and defining nanochannels; a microfluidics layer positioned upon the nanofluidics layer and defining microchannels; and biomolecular nanovalves providing bio-nanovalves which are moveable from a closed position to an open position to control fluid flow at a nanoscale. The micro/nano fabrication process for fabricating a multi-layer micro/nanofluidic flow device can comprise: forming a conductive layer on a substrate; forming an electrode pattern; forming a nanofluidics layer with a nanochannel pattern to ...

Method of Forming a Liquid-Liquid Mixing Phase Channel Group, Method of Controlling the Formation and Extinguishment of a Liquid-Liquid Mixing Phase Channel Group, And Module Therefor

A method of forming a liquid-liquid mixing phase channel group, which has the steps of: ejecting the first liquid as droplets into the phase of the second liquid in a two-liquid phase system in which two immiscible liquids oppose each other at an interface; incorporating the droplets of the first liquid into the phase of the first liquid, accompanied by the second liquid around the first liquid by allowing to collide the jet of the droplets with the interface; and forming a continuously connected microfluidic channel group in which the space between the layered droplets of the first liquid are filled with the second liquid in the liquid-liquid mixing phase that grows from the interface as a starting point. 1. A method of forming a liquid-liquid mixing phase channel group , comprising the steps of:ejecting the first liquid as droplets into the phase of the second liquid in a two-liquid phase system in which two immiscible liquids oppose each other at an interface,incorporating the droplets of the first liquid into the phase of the first liquid, accompanied by the second liquid around the first liquid by allowing to collide the jet of the droplets with the interface, andforming a continuously connected microfluidic channel group in which the space between the layered droplets of the first liquid are filled with the second liquid in the liquid-liquid mixing phase that grows from the interface as a starting point.2. A method of forming a liquid-liquid mixing phase channel group according to claim 1 , wherein the discharge of droplets of first liquid into the phase of second liquid is carried out by using a nozzle having a small tubes or pores.3. A method of forming a liquid-liquid mixing phase channel group according to claim 1 , wherein the flow in the continuously connected microfluidic channel group filled with the second liquid is occurred by sending the second liquid.4. A method of forming a liquid-liquid mixing phase channel group according to claim 1 , wherein ...

Microfluidic Device for the Generation of Combinatorial Samples

The present disclosure relates to a microfluidic device and a method allowing the generating and screening of combinatorial samples. A microfluidic device for producing droplets of at least one sample into an immiscible phase is provided, the device comprising a droplet maker connecting an immiscible phase channel and a sample channel having at least one sample inlet connected to at least one sample inlet channel injecting the at least one sample into the sample channel, wherein the injection of the at least one sample is controlled by at least one sample valve, so that the at least one sample flows either towards a sample waste outlet or into the at least one sample inlet channel, wherein different sample inlet channel of the at least one sample inlet channel have the same hydrodynamic resistance resulting from the length, height and width of each sample inlet channel upstream of the droplet maker. 1. A microfluidic device for producing droplets of at least one sample into an immiscible phase , the device comprising:a droplet maker connecting an immiscible phase channel and a sample channel having a plurality of sample inlet connected to a plurality of sample inlet channel injecting the at least one sample into the sample channel, wherein the injection of the at least one sample is controlled by a plurality of sample valves, so that the at least one sample flows either towards a sample waste outlet or into one of the plurality of sample inlet channels, wherein different ones of the plurality of sample inlet channels have the same hydrodynamic resistance resulting from the length, height and width of the sample inlet channel upstream of the droplet maker; and an outlet channel, wherein the hydrodynamic resistance of the outlet channel is lower than the hydrodynamic resistance of the immiscible phase channel.2. The microfluidic device of claim 1 , wherein ones of the plurality of sample inlet channels have a sample fluidic resistor to adjust length.3. The ...

Microfluidic devices, and methods of making and using the same

The present disclosure provides methods and systems for assaying a sample. A microfluidic device to perform an assay of a sample (e.g., biological sample) is described having a sample application site, a porous component and a flow channel. The porous component provides for uniform dissolution of a reagent and mixing of the sample and reagent without filtering the sample.

Microfabricated cellular traps based on three-dimensional micro-scale flow geometries

An improved microfluidic device and method of using the microfluidic device to measure natural motile response of a living moiety to a chemotactic agent. The invention comprises integrating microfluidic containment trenches within the microfluidic devices for cellular trapping, manipulation and real-time analysis of biological phenomena, including chemotaxis. The invention captures the design methodology for these traps, as well as the fabrication means and the associated external control mechanism that allows rapid, reversible and fully controlled changes in the chemical environment to which trapped cells are exposed.

SYSTEMS AND METHODS FOR HIGH THROUGHPUT SCREENING

Provided herein are compositions, systems, and methods for high throughput screening. In particular, provided herein are microfluidic devices for high throughput analysis of multiplex chemical (e.g., drug interactions) across a wide range of concentrations. 1. A microfluidic device , comprising:i) a routing layer comprising a plurality of microfluidic channels and a plurality of chemical reservoirs, wherein said microfluidic channel are in fluid communication with said chemical reservoirs;ii) a mixing layer comprising a plurality of biased tree mixers comprising microchannels of a plurality of different widths patterned therein and a plurality of sphere culture chambers; andiii) a lid layer comprising a plurality of chemical inlet holes, a plurality of cell inlet holes, and a plurality of vias.2. The device of claim 1 , wherein said vias are microfluidic channels claim 1 , punched holes claim 1 , or tubing that connect said lid claim 1 , said mixing layer claim 1 , and said routing layer.3. The device of claim 1 , wherein said routing layer forms the bottom layer of said device claim 1 , said mixing layer forms the middle layer of said device claim 1 , and said lid layer forms the top layer of said device.4. The device of claim 1 , wherein said microfluidic channels are 100 to 1000 μm in width and 100 to 1000 μm in height.5. The device of claim 4 , wherein said microfluidic channels are approximately 800 μm in width and 300 μm in height.6. The device of claim 1 , wherein sphere culture chambers are coated in a polyethylene glycol blocking agent.7. The device of claim 1 , wherein said mixing layer in configured to generate all possible drug combinations with a plurality of different mixing ratios of said chemicals for a plurality of different chemicals.8. The device of claim 7 , wherein said device is configured to generate at least 3 different mixing ratios of said chemicals.9. The device of claim 7 , wherein said device is configured to generate at least 5 ...

LIQUID MIXING APPARATUS

During the use of a liquid mixing apparatus, a liquid container can be installed onto an adapter. The liquid container drives a lifting assembly to allow a piercing assembly to pierce through the adapter. Once a fluid flows into a flowing passage portion via a fluid inlet, a portion of the fluid is pressurized via a branch passage portion with easy control and further flows into the liquid container to mix with a fragrance material inside the liquid container. The mixed fragrant liquid then flows out of a fluid outlet from the piercing assembly via the return passage portion and the flowing passage portion. With the pressurization design of the branch passage portion, the apparatus allows the increase of dimension to increase the flow rate while maintaining the pressurized liquid, which is convenient to use and facilitated for the control of the mixture amount with greater variation range. 1: A liquid mixing apparatus comprising:at least one connecting device having one side formed of at least one fluid inlet and another side formed of at least one fluid outlet;at least one flowing passage portion formed inside the connecting device and fluidly connected to the fluid inlet and the fluid outlet;at least one branch passage portion formed inside the connecting device and located at a side area of the flowing passage portion and fluidly connected thereto;at least one return passage portion formed inside the connecting device and located at the side area of the flowing passage portion and fluidly connected thereto;at least one lifting assembly moveably arranged on the connecting device;at least one piercing assembly moveably arranged on the lifting assembly, configured to move along therewith and located between the lifting assembly and the connecting device; the at least one piercing assembly comprising at least one first piercing member selectively connected to the branch passage portion and at least one second piercing member selectively connected to the return ...

FLUID MIXER

A fluid mixer includes a flow splitter and a mixing chamber. The flow splitter includes an inlet for receiving a flow of fluid and is configured to split the flow of fluid into first and second fluid streams. The second fluid stream has a higher density than the first fluid stream. The mixing chamber includes a first inlet, a second inlet and a mixing well. The second inlet is positioned below the first inlet. The second inlet of the mixing chamber is configured to receive the first fluid stream and the first inlet of the mixing chamber is configured to receive the second fluid stream to promote mixing of the first and second streams in the mixing well.

FLUID MIXING DEVICE

A fluid mixing device is provided with a plurality of flow channel units disposed to be divided in a plurality of layers. Each of the flow channel units has an inflow port, an outflow port, and a plurality of branch flow channels making the inflow port and the outflow port communicate with each other. The flow channel units located in different layers are connected to each other at the inflow port and the outflow port between the flow channel units, thereby configuring a three-dimensional flow channel as a whole. When the direction from the inflow port to the outflow path of each flow channel unit is set to be a flow direction in the flow channel unit, the flow directions intersect each other between the respective layers. 1. A fluid mixing device that mixes a plurality of fluids , comprising:a plurality of flow channel units disposed to be divided in a plurality of layers,wherein each of the plurality of flow channel units has one respective inflow port, one respective outflow port, and a plurality of branch flow channels making the respective inflow port and the respective outflow port communicate with each other,the respective inflow port of the respective flow channel unit on one side, out of the flow channel units which are located in different layers, and the respective outflow port of the respective flow channel unit on the other side are connected to each other, whereby a three-dimensional flow channel is configured such that a length of a flow channel through which the fluid flows is the same or substantially the same in any of the branch flow channels, andwhen a direction from the respective inflow port to the respective outflow port is set to be a flow direction of the fluid in each of the respective flow channel units, the flow directions intersect each other between the respective layers.2. The fluid mixing device according to claim 1 , wherein the flow directions in the connected flow channel units are orthogonal to each other.3. The fluid mixing ...

For the rapid production of droplets apparatus and method

RAPID PRODUCTION OF DROPLETS

The present invention generally relates to the production of fluidic droplets. Certain aspects of the invention are generally directed to systems and methods for creating droplets by flowing a fluid from a first channel to a second channel through a plurality of side channels. The fluid exiting the side channels into the second channel may form a plurality of droplets, and in some embodiments, at very high droplet production rates. In addition, in some aspects, double or higher-order multiple emulsions may also be formed. In some embodiments, this may be achieved by forming multiple emulsions through a direct, synchronized production method and/or through the formation of a single emulsion that is collected and re-injected into a second microfluidic device to form double emulsions. 147-. (canceled)48. An apparatus , comprising:a first microfluidic channel;a second microfluidic channel;at least five side microfluidic channels each connecting the first microfluidic channel with the second microfluidic channel; anda plurality of auxiliary microfluidic channels connecting to each of the at least five side microfluidic channels.49. The apparatus of claim 48 , comprising at least 10 side channels each connecting the first microfluidic channel with the second microfluidic channel.50. The apparatus of claim 48 , wherein each of the at least five side channels has a length of between about 90% and about 110% of the average length of the side channels.51. The apparatus of claim 48 , wherein the first microfluidic channel and the second microfluidic channel have a distance of separation that is between about 90% and about 110% of the average distance of separation.52. The apparatus of claim 48 , wherein the at least five side channels are positioned such that the distance of separation between any neighboring side channels is between 90% and about 110% of the average distance of separation between neighboring side channels.53. The apparatus of claim 52 , wherein the at least ...

Active electroosmosis-extraction micro hybrid integration device and extraction integration method thereof

High Pressure Homogenizer And Method For Manufacturing Graphene Using The Same

The present invention relates to a high pressure homogenizer and a method for manufacturing graphene using the same, and according to one aspect of the present invention, there is provided a high pressure homogenizer comprising a channel module which comprises a microchannel through which an object for homogenization passes, wherein the channel module comprises at least one baffle disposed so as to partition the microchannel into a plurality of spaces and the baffle is provided so as to partition the microchannel into two spaces along the width direction or the height direction.

Microfluidic mixer having channel width variation for enhanced fluid mixing

A micromixing apparatus includes a mixing microchannel formed in a top surface of a substrate having a channel length and a variable channel width defined by a first sidewall surface and an opposing second sidewall surface. The channel width varies from a minimum channel width h to a maximum channel width H in a ratio of H:h>=1.1:1.0. A first inlet is for injecting a first fluid into the mixing microchannel and a second inlet for injecting a second fluid into the mixing microchannel. The first and second fluid flow in a flow direction in the mixing microchannel along the channel length. The first sidewall surface includes first curved surface portions and the second sidewall surface includes a second curved surface portions. The plurality of first curved surface portions and plurality of second curved surface portions are non-overlapping to provide the variable channel width.

MICROFLUIDIC DEVICES, AND METHODS OF MAKING AND USING THE SAME

The present disclosure provides methods and systems for assaying a sample. A microfluidic device to perform an assay of a sample (e.g., biological sample) is described having a sample application site, a porous component and a flow channel. The porous component provides for uniform dissolution of a reagent and mixing of the sample and reagent without filtering the sample. 130-. (canceled)31. A method comprising: a flow channel in fluid communication with the sample application site; and', 'a porous component positioned between the sample application site and flow channel, wherein the porous component comprises a porous matrix and an assay reagent;, 'contacting a sample to a sample application site of a microfluidic device, the microfluidic device comprisingilluminating the sample in the flow channel with a light source; anddetecting light from the sample.32. The method according to claim 31 , wherein the sample mixes with the assay reagent by flowing the sample through the porous matrix.33. The method according to claim 31 , wherein mixing of the sample with the assay reagent comprises labeling one or more components of the sample with a detectable label.34. The method according to claim 33 , wherein labeling comprises coupling one or more components to an analyte-specific binding member.35. The method according to claim 34 , wherein the analyte-specific binding member is conjugated to an optically detectable label.36. The method according to claim 34 , wherein the analyte-specific binding member is an antibody or antibody fragment.37. The method according to claim 36 , wherein the antibody or antibody fragment specifically binds to a target selected from CD14 claim 36 , CD4 claim 36 , CD45RA claim 36 , CD3 or a combination thereof.38. The method according to claim 35 , wherein the optically detectable label comprises a fluorescent dye.39. The method according to claim 38 , wherein the fluorescent dye comprises a compound selected from the group consisting of ...

Rapid production of droplets

The present invention generally relates to the production of fluidic droplets. Certain aspects of the invention are generally directed to systems and methods for creating droplets by flowing a fluid from a first channel to a second channel through a plurality of side channels. The fluid exiting the side channels into the second channel may form a plurality of droplets, and in some embodiments, at very high droplet production rates. In addition, in some aspects, double or higher-order multiple emulsions may also be formed. In some embodiments, this may be achieved by forming multiple emulsions through a direct, synchronized production method and/or through the formation of a single emulsion that is collected and re-injected into a second microfluidic device to form double emulsions.

Fluid mixer

A fluid mixer includes a flow splitter and a mixing chamber. The flow splitter includes an inlet for receiving a flow of fluid and is configured to split the flow of fluid into first and second fluid streams. The second fluid stream has a higher density than the first fluid stream. The mixing chamber includes a first inlet, a second inlet and a mixing well. The second inlet is positioned below the first inlet. The second inlet of the mixing chamber is configured to receive the first fluid stream and the first inlet of the mixing chamber is configured to receive the second fluid stream to promote mixing of the first and second streams in the mixing well.

Fluid mixer

A fluid mixer includes a flow splitter and a mixing chamber. The flow splitter includes an inlet for receiving a flow of fluid and is configured to split the flow of fluid into first and second fluid streams. The second fluid stream has a higher density than the first fluid stream. The mixing chamber includes a first inlet, a second inlet and a mixing well. The second inlet is positioned below the first inlet. The second inlet of the mixing chamber is configured to receive the first fluid stream and the first inlet of the mixing chamber is configured to receive the second fluid stream to promote mixing of the first and second streams in the mixing well.