АВТОМАТИЗИРОВАННЫЙ ХИМИЧЕСКИЙ СИНТЕЗАТОР И СПОСОБ СИНТЕЗА С ЕГО ИСПОЛЬЗОВАНИЕМ

... 1. Автоматизированный химический синтезатор, содержащий: ! a) корпус, имеющий верхнюю, нижнюю и боковые стенки, определяющие границы внутреннего пространства корпуса, и опорный элемент, расположенный во внутреннем пространстве корпуса; ! b) неподвижную систему подачи, расположенную на опорном элементе и содержащую одно или несколько устройств подачи реагента и одно или несколько устройств подачи промывочной жидкости; и ! c) поворотное карусельное устройство, на котором расположена одна или несколько реакционных ячеек, помещенное под неподвижной системой подачи для обеспечения вращательного и последовательного перемещения реакционной ячейки до положения выравнивания относительно устройства подачи реагента и устройства подачи промывочной жидкости для приема в указанном положении одного из реагентов и промывочной жидкости в соответствии с этапами заданного протокола синтеза. ! 2. Автоматизированный химический синтезатор по п.1, дополнительно содержащий приводное устройство, содержащее приводной ...

DEVICE FOR SIMULTANEOUSLY CARRYING OUT A PLURALITY OF CHEMICAL REACTION STEPS

SYNTHESIS MECHANISM FOR OLIGONUKLEOTIDE UNDER USE OF CENTRIFUGAL FORCE

Oligonucleotide synthesizer

An apparatus for high-throughput combinatorial syntheses of organic molecules including a reaction vessel for containing a combinatorial-chemistry synthetic reaction, a liquid dispenser for dispensing the liquid, a liquid aspirator and an adjustment mechanism. The reaction vessel includes an ingress aperture allowing a liquid to enter into an interior of the vessel and an egress aperture for aspirating the liquid from the vessel. The liquid dispenser dispenses liquid through the ingress aperture. The liquid aspirator aspirates liquid through the egress aperture and includes a rotor for carrying the vessel and orbiting the vessel about an axis of rotation. The rotor is oriented generally in a horizontal plane and includes an adjustment mechanism for adjusting the angle of the vessel relative to the horizontal plane in response to the centrifugal force generated by orbiting the vessel about the axis of rotation. A method of combinatorial synthesis of organic molecules is also disclosed.

APPARATUS AND METHOD FOR CONCURRENT CHEMICAL SYNTHESIS

This invention provides an apparatus for preparing chemical libraries. The apparatus includes: (1) a carousel comprising a plurality of reaction mounts having at least one reaction well; (2) a rotator that rotates the carousel step-wise; (3) a fluid delivery system; (4) a drain system; and (5) a programmable computer that controls the operation of the apparatus, including the rotator, the fluid delivery system, the drain system and other systems in the apparatus. The preparation of chemical libraries involves rotating the carousel through a plurality of stations. At each station, a physical step in a reaction protocol is carried out on the reaction wells of the mount docked at the station.

APPARATUS AND METHOD FOR USE IN SOLID PHASE CHEMICAL SYNTHESIS

An apparatus is disclosed for use in solid-phase chemical synthesis procedures. The apparatus allows the separation of a cleaved product from the solid-phase used to make the product, and subsequent removal of the solid- phase from the apparatus. The apparatus allows the cleaved product to be concentrated within the apparatus without transfer to another device.

METHOD AND APPARATUS FOR CONDUCTING AN ASSAY

The present invention relates to methods and apparatus for conducting an assay. In particular, the present invention relates to a rotatable platform which can be used for conducting an assay, in particular multi-step assays. The present invention provides a rotatable platform adapted to immobilise a first binding partner in one or more discrete areas on a surface of said platform, or to selectively immobilise a second binding partner in one or more discrete areas on a surface of said platform. The invention also relates to methods, apparatus, a kit and the use of the rotatable platform for conducting an assay. In particular, the invention has been developed primarily for use in sequencing nucleic acid by pyrosequencing, however the invention is not limited to this field.

MULTI-WELL ROTARY SYNTHESIZER

An apparatus (100) for synthesizing polymer chains includes a controller (480), a plurality of precision fit vials (160, 181) circularly arranged in multiple banks (115, 125, 180) on a cartridge (170, 300), a drain (740, 750, 760, 770) corresponding to each bank of vials, a chamber bowl (400), a plurality of valves (110, 120, 130, 470) for delivering reagents to selective vials (160, 181), and a waste tube system (430, 530) for purging material from the vials. A purging operation can be selectively performed on one or more of the banks of vials. The multiple banks of valves provide an additional number of reagent choices while operating in a serial mode and faster reagent distribution while operating in a parallel mode. The plurality of vials are stored in the cartridge and are divided among individual banks wherein each bank of vials has a corresponding drain. There is at least one waste tube system for expelling the reagent solution from vials within a particular bank of vials when the ...

Multiple bioreactor device.

HIGH THROUGHPUT MECHANICAL ALLOYING AND SCREENING

The invention provides an apparatus and methods for conducting ball milling operations in a high throughput manner. Also provided are devices and methods for high throughput screening of alloys and other materials to identify those that have desired gas storage properties.

MEHRFACH-BIOREAKTORVORRICHTUNG

Vorrichtung zum Entfernen einer Füllung aus einer Reaktionskammer

Multi-station reaction apparatus

MULTIPLE BIOREACTOR DEVICE

DEVICE TO THE PARALLEL EXECUTION OF A MULTIPLICITY OF CHEMICAL REACTION SEQUENCES.

Method and device for the continuous testing of materials

AUTOMATED CHEMICAL SYNTHESIZER AND METHOD FOR SYNTHESIS USING SAME

An automated synthesizer is disclosed in which the reaction wells are moved by a carrier into alignment with stationary fluid delivery stations that are in communication with a source of reactants and/or wash solutions. In an alternate embodiment the delivery stations are moved into alignment with stationary reaction wells. A method for synthesizing chemical compounds employing the synthesizer is disclosed.

Mixing and reacting of solids, suspensions or emulsions in a microwave field

A supported catalyst comprising a mixed metal oxide is useful for the vapor phase oxidation of an alkane or a mixture of an alkane and an alkene to an unsaturated carboxylic acid and for the vapor phase ammoxidation of an alkane or a mixture of an alkane and an alkene to an unsaturated nitrile. The mixed oxide catalyst is prepared by depositing a plurality of films of different elements using physical or chemical vapor deposition.

DETECTION OF NUCLEIC ACID SEQUENCE DIFFERENCE USING LIGASE DETECTION REACTION WITH ADDRESSABLE ARRAY

PROBLEM TO BE SOLVED: To provide a method for identifying one or more of a plurality of sequences differing by one or more single base changes, insertions, deletions, or translocations in a plurality of target nucleotide sequences. SOLUTION: The ligation phase utilizes a ligation detection reaction between one oligonucleotide probe, which has a target sequence-specific portion and an addressable array-specific portion, and a second oligonucleotide probe, having a target sequence-specific portion and a detectable label. After the ligation phase, the capture phase is carried out by hybridizing the ligated oligonucleotide probes to a solid support with an array of immobilized capture oligonucleotides at least some of which are complementary to the addressable array-specific portion. Following completion of the capture phase, a detection phase is carried out to detect the labels of ligated oligonucleotide probes hybridized to the solid support. The present invention also relates to a kit for ...

Verfahren, Verwendung des Verfahrens sowie Verwendung eines Mikrowellenheizgeräts zum Mischen und zur Auslösung von chemischen Reaktionen von Feststoffgemischen oder Suspensionen in einem Mikrowellenfeld

Verfahren zum Mischen und zur Auslösung von chemischen Reaktionen von Feststoffgemischen oder Suspensionen in einem Mikrowellenfeld, wobei das Verfahren die folgenden Schritte aufweist: - Einbringen der Feststoffgemische oder Suspensionen in wenigstens einen mikrowellen-transparenten Behälter, - Drehen des Behälters unter Einwirkung einer Mikrowellenstrahlung, wobei die Drehachse bezüglich der Senkrechten um einen Winkel geneigt ist zum homogenen Mischen der eingebrachten Feststoffgemische oder Suspensionen, und dadurch - Auslösen einer chemischen Reaktion der vorab eingebrachten Feststoffgemische oder Suspensionen.

PROCEDURE AND DEVICE FOR THE PARALLEL PRODUCTION OF OLIGONUKLEOTIDEN

FIBER MATRIX TO BRINGING TOGETHER CHEMICAL MATERIALS, AS WELL AS PROCEDURE FOR THE PRODUCTION AND USE OF IT

Device for carrying out chemical reactions

CONTINUOUS POLYMER SYNTHESIZER

Described is a system and method for synthesizing polymeric molecules such as oligonucleotides and polypeptides. The system is capable of continuously synthesizing molecules by providing an array of reaction sites and an array of stations for carrying out synthetic manipulations. The reaction sites in the former array can be placed in a fixed order and at fixed intervals relative to each other. Similarly, the stations can be placed in a fixed order and at fixed intervals relative to each other. The two arrays can be moved relative to each other such that the stations carry out desired steps of a reaction scheme at each reaction site. The relative locations of the stations and the schedule for the relative movement can correlate with the order and duration of reaction steps in the reaction scheme such that once a reaction site has completed a cycle of interacting with the full array of stations then the reaction scheme is complete.

Method and Apparatus for Conducting an Assay

The present invention relates to methods and apparatus for conducting an assay. In particular, the present invention relates to a rotatable platform which can be used for conducting an assay, in particular multi-step assays. The present invention provides a rotatable platform adapted to immobilise a first binding partner in one or more discrete areas on a surface of said platform, or to selectively immobilise a second binding partner in one or more discrete areas on a surface of said platform. The invention also relates to methods, apparatus, a kit and the use of the rotatable platform for conducting an assay. In particular, the invention has been developed primarily for use in sequencing nucleic acid by pyrosequencing, however the invention is not limited to this field.

MULTIPLE BIO-REACTOR DEVICE

Apparatus and method for concurrent chemical synthesis

COMBINATORIAL CHEMICAL SYNTHESIS

Combinational synthesis methods obtain a plurality of compositions having materially different characteristics using an apparatus having a plurality of collectors. A first quantity of fluid reactants are reacted to form a first quantity of production composition. Following completion of the collection of the first quantity of product composition, a second quantity of fluid reactants are reacted to form a second quantity of product composition materially different from the first quantity of product composition. An apparatus includes a nozzle (130) connected to a reactant source (120) and a plurality of collectors (236). The nozzle and plurality of collectors move relative to each other such that a collector can be selectively placed to receive a fluid stream emanating from the nozzle. The plurality of product compositions can be evaluated to determine their suitability for various applications. © KIPO & WIPO 2007 ...

Oligonucleotide synthesizer

An apparatus for high-throughput combinatorial syntheses of organic molecules including a reaction vessel for containing a combinatorial-chemistry synthetic reaction, a liquid dispenser for dispensing the liquid, a liquid aspirator and an adjustment mechanism. The reaction vessel includes an ingress aperture allowing a liquid to enter into an interior of the vessel and an egress aperture for aspirating the liquid from the vessel. The liquid dispenser dispenses liquid through the ingress aperture. The liquid aspirator aspirates liquid through the egress aperture and includes a rotor for carrying the vessel and orbiting the vessel about an axis of rotation. The rotor is oriented generally in a horizontal plane and includes an adjustment mechanism for adjusting the angle of the vessel relative to the horizontal plane in response to the centrifugal force generated by orbiting the vessel about the axis of rotation. A method of combinatorial synthesis of organic molecules is also disclosed.

Kartusche für eine Vorrichtung zum Durchführen chemischer oder biologischer Reaktionen

An apparatus for the chemical synthesis of oligonucleotides

Apparatus for carrying out a multiplicity of different sequential chemical syntheses on solid-supports simultaneously comprises a multiplicity of complementary support plates (15-26) each formed with a reaction chamber (39) opening through the plate and containing the solid-support (42) together with at least one by-pass channel (35-38) through the plate. The by-pass channel or channels (35-38) and reaction chamber (39) are disposed equi-angularly and at the same radial distance about an axis (33) of the plate (15). The plates (15-26) are rotatably supported in compression in face to face contact between end plates (13, 14) complementary to the plates (15-26) and each formed with a blank position and passages for at least one reactant stream. Means is provided for independently rotating each plate whereby each reaction chamber (39) of each plate (15-26) may independently be isolated or positioned in the or a selected reactant stream.

CHEMICAL SYNTHESIS OF OLIGONUCLEOTIDES

Method and apparatus for conducting an assay

The present invention relates to methods and apparatus for conducting an assay. In particular, the present invention relates to a rotatable platform which can be used for conducting an assay, in particular multi-step assays. The present invention provides a rotatable platform adapted to immobilise a first binding partner in one or more discrete areas on a surface of said platform, or to selectively immobilise a second binding partner in one or more discrete areas on a surface of said platform. The invention also relates to methods, apparatus, a kit and the use of the rotatable platform for conducting an assay. In particular, the invention has been developed primarily for use in sequencing nucleic acid by pyrosequencing, however the invention is not limited to this field.

MULTI CHAMBER REACTOR

1. Claims for the Contracting States : BE, GB, NL, AT An apparatus for perfoming chemical reaction sequences, characterized by a first number n of reaction discs (1) which are superposed in the form of a stack and are individually displaceable relative to one another, means for defining the displacement in a second number m of identical steps, m continuous bores (11, 14) disposed in the discs at the step distances from one another, one of the bores being widened out per disc to form a reaction chamber (14), means for retaining a support material in the reaction chamber on the flow of a reagent there-through. 1. Claims for the Contracting States : DE, FR, IT, CH, LI, SE An apparatus for performing chemical reaction sequences having a first number n of reaction discs (1) which are superposed in the form of a stack and are individually displaceable relative to one another, means for defining the displacement in a second number m of identical steps, m continuous bores (11, 14) disposed in the discs at the step distances from one another, one of the bores being widened out per disc to form a reaction chamber (14), means for retaining a support material in the reaction chamber on the flow of a reagent therethrough, characterized in that the undersides of the reaction discs have grooves extending concentrically to the bores and containing O-rings.

APPARATUS FOR THE PARALLEL PERFORMANCE OF A PLURALITY OF CHEMICAL REACTION SEQUENCES

A B S T R A C T Apparatus for performing chemical reaction sequences comprising a first number n of preferably circular reaction discs (1) superposed in the form of a stack and individually displaceable relatively to one another, means for defining the displacement in a second number m of identical steps, m continuous bores (11, 14) disposed in the discs at the step distances from one another, one bore being widened out per disc to form a reaction chamber (14), and means for retaining a support material in the reaction chamber on the flow of a reagent there-through.

FIBER ARRAY FOR CONTACTING CHEMICAL SPECIES AND METHODS FOR USING AND MAKING SAME

... ▓▓▓An apparatus and method are provided for contacting at least two chemical ▓species, comprising a support plate having a channel for receiving a mobile ▓chemical species and a fiber, having a second chemical species immobilized ▓thereon, disposed on the support plate. At least a portion of the fiber is ▓exposed to the channel such that the mobile chemical species is capable of ▓contacting the second chemical species. An apparatus and method for reading ▓the fiber array, an apparatus and method for making the fiber array, and ▓methods of using the fiber array of the present invention are also provided.▓ ...

COMPOUND PROFILING DEVICES, SYSTEMS, AND RELATED METHODS

High throughput compound profiling systems, and related devices and sub- systems that can be used to perform various compound profiling processes are provided. These systems typically include work perimeters (202, 204, 206) that are organized for optimum efficiency and processing accuracy. Further, these systems are readily adaptable for performing a wide array of assays, as many different system components are easily incorporated or interchangeable in a particular system. System components that are provided by the invention include cell culture dissociators, which can be used, e.g., to effect cell wetting, dissociation, and/or agitation applications. In some embodiments, these cell culture dissociators are included as components of automated cell culture passaging stations. Dispensing devices that permit on- the- fly fluid temperature regulation are also provided. In addition, various compound profiling methods, cell dissociation methods, uniform cell concentration dispensing methods, ...

ROTARY COMBINATED APPARATUS OF MULTIPHASE REACTION AND REGENERATION

REACTION VESSEL AND ANALYZER

A reaction container and an analyzing apparatus that hold a liquid agitated by sound waves. The reaction container (7) has two openings (8c) serving as an introduction/discharge port of a liquid, and a holding member (8) having the two openings, holding the liquid between the two openings, and including a sound wave generator arranged on a side surface or a portion close to the side surface for emitting sound waves to a portion between the openings to agitate the liquid. Two openings (8c) are arranged opposite to each other. The holding member (8) is arranged in a vertical direction, and a lower one of the openings (8c) in the vertical direction has a smaller area than an upper one of the openings (8c) in the vertical direction.

Apparatus and method for use in solid phase chemical synthesis

CHEMICAL SYNTHESIS

METHOD AND DEVICE FOR THE CONTINUOUS TESTING OF MATERIALS

Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays

METHOD AND APPARATUS FOR CONDUCTING AN ASSAY

APPARATUS AND METHOD FOR USE IN SOLID PHASE CHEMICAL SYNTHESIS

An apparatus is disclosed for use in solid-phase chemical synthesis procedures. The apparatus allows the separation of a cleaved product from the solid-phase used to make the product, and subsequent removal of the solid-phase from the apparatus. The apparatus allows the cleaved product to be concentrated within the apparatus without transfer to another device.

Fläschchen und Mehrgefäß rotierende Synthesevorrichtung

Device for the continuous testing of materials

DETECTION OF NUCLEIC ACID SEQUENCE DIFFERENCES USING THE LIGASE DETECTION REACTION WITH ADDRESSABLE ARRAYS

The present invention describes a method for identifying one or more of a plurality of sequences differing by one or more single base changes, insertions, deletions, or translocations in a plurality of target nucleotide sequences. The ligation phase utilizes a ligation detection reaction between one oligonucleotide probe which has a target sequence-specific portion and an addressable array-specific portion, and a second oligonucleotide probe, having a target sequence specific portion and a detectable label. After the ligation phase, the capture phase is carried out by hybridizing the ligated oligonucleotide probes to a solid support with an array of immobilized capture oligonucleotides at least some of which are complementary to the addressable array-specific portion. Following completion of the capture phase, a detection phase is carried out to detect the labels of ligated oligonucleotide probes hybridized to the solid support. The ligation phase can be preceded by an amplification process ...

MULTI-WELL ROTARY SYNTHESIZER

An apparatus (100) for synthesizing polymer chains includes a controller (480), a plurality of precision fit vials (160, 181) circularly arranged in multiple banks (115, 125, 180) on a cartridge (170, 300), a drain (740, 750, 760, 770) corresponding to each bank of vials, a chamber bowl (400), a plurality of valves (110, 120, 130, 470) for delivering reagents to selective vials (160, 181), and a waste tube system (430, 530) for purging material from the vials. A purging operation can be selectively performed on one or more of the banks of vials. The multiple banks of valves provide an additional number of reagent choices while operating in a serial mode and faster reagent distribution while operating in a parallel mode. The plurality of vials are stored in the cartridge and are divided among individual banks wherein each bank of vials has a corresponding drain. There is at least one waste tube system for expelling the reagent solution from vials within a particular bank of vials when the ...

APPARATUS AND METHOD FOR USE IN SOLID PHASE CHEMICAL SYNTHESIS

An apparatus is disclosed for use in solid-phase chemical synthesis procedures. The apparatus allows the separation of a cleaved product from the solid-phase used to make the product, and subsequent removal of the solid- phase from the apparatus. The apparatus allows the cleaved product to be concentrated within the apparatus without transfer to another device.

MULTIPLE BIO-REACTOR DEVICE

A multiple bio-reactor device comprising a plurality of reactor segments (10, 10a, 10b), wherein a reaction vessel (12) and at least one supply vessel (14a, 14b, 14c, 14d) can be secured to each reactor segment in a medium-tight suspended manner. A plurality of processing stations (16a, 16b, 16c, 16d, 16e, 16f, 16g, 16h) is provided in order to carry out predetermined processing steps with respect to the reaction vessel and/or supply vessels of each reactor segment, wherein movement means are provided in order to bring each reactor segment to a respective processing station or vice-versa. A respective reactor segment comprises a sterilizable area which encompasses at least the reaction vessel (12) and part of the reactor segment located thereabove.

Automated peptide production machine

A device capable of synthesizing a plurality of selected peptides by automatically mixing various amino acids, solvents, and activators and adding these to resins contained in a plurality of individual reaction vessels. A plurality of amino acids are contained in vessels within a carousel which is rotated into position where a syringe is inserted into a selected vessel to transport the amino acid within to a pre-reaction vessel for mixing with other selected amino acids which were previously drawn from the carousel. The mixture of amino acids is then transported to a reaction vessel containing the resin balls for growth of the selected peptide. The device includes a computer, controllable valves, at least one pump, pressurized gas such as nitrogen for transporting fluids, various vessels containing amino acids, solvents, activators, resins, and tubing connecting these elements. The computer is programmable to sample, mix selected components, and apply the mixture to resins for growing peptides ...

Apparatus and method for concurrent chemical synthesis

Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays

DEVICE FOR THE CONTINUOUS TESTING OF MATERIALS

HIGH THROUGHPUT MECHANICAL ALLOYING AND SCREENING

The invention provides an apparatus and methods for conducting ball milling operations in a high throughput manner. Also provided are devices and methods for high throughput screening of alloys and other materials to identify those that have desired gas storage properties.

APPARATUS AND METHOD FOR USE IN SOLID PHASE CHEMICAL SYNTHESIS

An apparatus is disclosed for use in solid-phase chemical synthesis procedures. The apparatus allows the separation of a cleaved product from the solid-phase used to make the product, and subsequent removal of the solid-phase from the apparatus. The apparatus allows the cleaved product to be concentrated within the apparatus without transfer to another device.

AUTOMATED OLIGOMER SYNTHESIS

L'invention concerne des systèmes comprenant un appareil et des procédés de synthèse automatisée d'oligomères.

METHOD AND DEVICE FOR THE CONTINUOUS TESTING OF MATERIALS

The present invention relates to a method for testing building blocks, which are identical or different, of a library of materials, comprising at least two building blocks, for performance characteristics, comprising a sequence of the following steps: 4) testing of at least one library building block for at least one performance characteristic; 5) detecting at least one measurable quantity, to which at least one performance characteristic of the at least one library building block can be assigned by at least one sensor, wherein at least one of the steps (4) and (5) is performed continuously.

DETECTION OF NUCLEIC ACID SEQUENCE DIFFERENCES USING THE LIGASE DETECTION REACTION WITH ADDRESSABLE ARRAYS

The present invention describes a method for identifying one or more of a plurality of sequences differing by one or more single base changes, insertions, deletions, or translocations in a plurality of target nucleotide sequences. The ligation phase utilizes a ligation detection reaction between one oligonucleotide probe which has a target sequence-specific portion and an addressable array-specific portion, and a second oligonucleotide probe, having a target sequence specific portion and a detectable label. After the ligation phase, the capture phase is carried out by hybridizing the ligated oligonucleotide probes to a solid support with an array of immobilized capture oligonucleotides at least some of which are complementary to the addressable array-specific portion. Following completion of the capture phase, a detection phase is carried out to detect the labels of ligated oligonucleotide probes hybridized to the solid support. The ligation phase can be preceded by an amplification process ...

Multi-station reaction apparatus

SYSTEM, METHOD, AND DEVICE FOR HIGH-THROUGHPUT, AUTOMATED CULTURING OF GENETICALLY MODIFIED ORGANISMS

A fluid transfer system includes a transfer carousel capable of rotational and/or translational movement; at least one holding vessel (e.g. syringe) having a plunger, wherein the syringe is connected to the transfer carousel such that the movement of the transfer carousel results in movement of the syringe and wherein the syringe is capable of translational movement relative to the transfer carousel; a drive motor connected to the syringe that is capable of controlling the position of the plunger; and a peripheral module comprising at least one vessel that is capable of containing a fluid, wherein the vessel has an opening that can be mated with the syringe to allow fluid transfer between the vessel and the syringe. Methods for transferring a fluid are also disclosed.

System for controlling and optimizing reactions in solid phase synthesis of small molecules

A instrument for performing synthesis of small molecules such as Oligos and Peptides when using solid phase synthesis techniques to synthesize small molecules. The system and methods include a mechanism for controlling the pressure differential across the reaction vessels that contain the solid support used in solid phase synthesis. Reaction vessels are held in a holder that provides a sealable chamber at the outlet ends of the reaction vessels. The rotor containing the reaction vessels is placed within a sealable chamber. The sealable chamber is fitted with a means for engaging the rotor and draining the reaction vessels to waste. The sealable chamber is also fitted with a means for engaging the rotor to drain the reaction vessels at a slower, variable rate.

APPARATUS FOR PERFORMING CONTINUOUS CHEMICAL REACTION

Reaktionsgefäßaufnahme für die Festphasensynthese

Für eine Vorrichtung (12) zum Durchführen einer Parallelsynthese, insbesondere eine Festphasensynthese, wird zum einfachen und einen guten Wärmekontakt herstellenden Befestigen der Reaktionsgefäße (1) in den Aufnahmen (13) vorgeschlagen, daß die Reaktionsgefäße (1) aus einem elastischen Material gebildet sind und mittels einer Schnappverbindung in die Aufnahmen (13) eingesetzt werden.

Methods and apparatus for universal fluid exchange

High throughput mechanical alloying and screening

Fiber array for contacting chemical species and methods for using and making same

Chemical reactor with tempering means

Multi-well rotary synthesizer

OLIGONUCLEOTIDE SYNTHESIZER

An apparatus for high-throughput combinatorial syntheses of organic molecules including a reaction vessel for containing a combinatorial-chemistry synthetic reaction, a liquid dispenser for dispensing the liquid, a liquid aspirator and an adjustment mechanism. The reaction vessel includes an ingress aperture allowing a liquid to enter into an interior of the vessel and an egress aperture for aspirating the liquid from the vessel. The liquid dispenser dispenses liquid through the ingress aperture. The liquid aspirator aspirates liquid through the egress aperture and includes a rotor for carrying the vessel and orbiting the vessel about an axis of rotation. The rotor is oriented generally in a horizontal plane and includes an adjustment mechanism for adjusting the angle of the vessel relative to the horizontal plane in response to the centrifugal force generated by orbiting the vessel about the axis of rotation. A method of combinatorial synthesis of organic molecules is also disclosed.

High throughput mechanical alloying and screening

The invention provides an apparatus and methods for conducting ball milling operations in a high throughput manner. Also provided are devices and methods for high throughput screening of alloys and other materials to identify those that have desired gas storage properties.

Oligonucleotide synthesizer

An apparatus for high-throughput combinatorial syntheses of organic molecules including a reaction vessel for containing a combinatorial-chemistry synthetic reaction, a liquid dispenser for dispensing the liquid, a liquid aspirator and an adjustment mechanism. The reaction vessel includes an ingress aperture allowing a liquid to enter into an interior of the vessel and an egress aperture for aspirating the liquid from the vessel. The liquid dispenser dispenses liquid through the ingress aperture. The liquid aspirator aspirates liquid through the egress aperture and includes a rotor for carrying the vessel and orbiting the vessel about an axis of rotation. The rotor is oriented generally in a horizontal plane and includes an adjustment mechanism for adjusting the angle of the vessel relative to the horizontal plane in response to the centrifugal force generated by orbiting the vessel about the axis of rotation. A method of combinatorial synthesis of organic molecules is also disclosed.

Device for performing multiple chemical reactions and processes in high frequency fields

A magazine-type sample rotation body (7) is arranged in a high frequency chamber (5) so that it rotates away from the horizontal, the body (7) accommodating, in fixed location, reactor blocks (10) that are sealed or sealable from fluids such that fluids contained in sample vessels in the reactor blocks can be mixed before, during and after high frequency exposure because of the rotational movement of the sample rotation body.

Oligonucleotide synthesizer

An apparatus for high-throughput combinatorial syntheses of organic molecules including a reaction vessel for containing a combinatorial-chemistry synthetic reaction, a liquid dispenser for dispensing the liquid, a liquid aspirator and an adjustment mechanism. The reaction vessel includes an ingress aperture allowing a liquid to enter into an interior of the vessel and an egress aperture for aspirating the liquid from the vessel. The liquid dispenser dispenses liquid through the ingress aperture. The liquid aspirator aspirates liquid through the egress aperture and includes a rotor for carrying the vessel and orbiting the vessel about an axis of rotation. The rotor is oriented generally in a horizontal plane and includes an adjustment mechanism for adjusting the angle of the vessel relative to the horizontal plane in response to the centrifugal force generated by orbiting the vessel about the axis of rotation. A method of combinatorial synthesis of organic molecules is also disclosed.

Mixing and reacting of solids, suspensions or emulsions in a microwave field

DEVICE FOR THE CONTINUOUS TESTING OF MATERIALS

The present invention relates to a device for the continuous testing of at least two building blocks, which are part of a combinatorial material library. Thereby, said device is in particular characterized in that it comprises at least the following constituent parts: (i) at least one spatially stationary component with at least one means for supply, (ii) at least one spatially non-stationary component as well as (iii) at least one unit for the uptake of a building block. Thereby, at least one building block moves spatially relative to the at least one other building block during the testing.

METHOD AND APPARATUS FOR CONDUCTING AN ASSAY

The present invention relates to methods and apparatus for conducting an assay. In particular, the present invention relates to a rotatable platform which can be used for conducting an assay, in particular multi-step assays. The present invention provides a rotatable platform adapted to immobilise a first binding partner in one or more discrete areas on a surface of said platform, or to selectively immobilise a second binding partner in one or more discrete areas on a surface of said platform. The invention also relates to methods, apparatus, a kit and the use of the rotatable platform for conducting an assay. In particular, the invention has been developed primarily for use in sequencing nucleic acid by pyrosequencing, however the invention is not limited to this field.

Substrate measuring method and device

To provide a method of making measurements for a sample on the measuring surfaces of a substrate which makes it possible to simplify the control and construction of a measuring device, shorten the measuring period, make the measuring conditions constant, and improve the positional accuracy. The method and a device for carrying out the method are characterized in that measurements for the sample is performed by forming a circular orbit of detection areas, where detection is performed with a detector, on the measuring surfaces of the substrate while moving the detection areas relative to the substrate.

APPARATUS FOR SIMULTANEOUSLY PERFORMING PLURAL REACTIONS

PURPOSE: To provide the titled apparatus enabling simultaneous synthesis of different kinds of plural DNA, etc., by stacking a plurality of flat-plate rotors each having plural circular flow channels as reaction chambers and providing the stacked rotors with rearrangeable liquid flow channels, rotating means for the rotors and liquid- feeding means. CONSTITUTION: The present apparatus 1 contains a plurality of stacked flat-plate rotors 2 each having plural circular flow channels 4, 8. Either one of the channels plays the role of reaction chamber 3 to hold a material to be reacted. A plurality of rearrangeable interconnected flow channels 4 are formed by a reaction part 6 formed by the rotors 2. These rotors 2 are independently rotated around a central axis 5 under sliding action by a rotating means. The apparatus is further provided with a group of liquid feeding lines 10 to feed different kinds of specific liquid and with a group of washing liquid feeding line 11 to feed the same washing ...

Kartusche für eine Vorrichtung zur Durchführung chemischer oder biologischer Reaktionen

AN APPARATUS FOR THE CHEMICAL SYNTHESIS OF OLIGONUCLEOTIDES

Multi-station reaction apparatus

A multi-station reaction apparatus has a heat transfer block 30 and a removable cooling module 4 with channels in which a coolant can circulate. Also disclosed is a multi-station reaction apparatus with a heat transfer block 30 with a central recess (32,fig.3a), and the corresponding block itself 30. Further disclosed is a cooling module 4 with a central recess (fig.2a). Lastly disclosed is a combination of an integrally formed heat transfer block and cooling module. The first of the inventions described may provide an easier way to cool reaction vessels other than removal of the vessels, insertion of an annular tray containing ice, and insertion of the bottoms of the reaction vessels into the ice.

AN APPARATUS FOR THE CHEMICAL SYNTHESIS OF OLIGONUCLEOTIDES

Fiber array for contacting chemical species and methods for using and making same

Oligonucleotide synthesizer using centrifugal force

COMBINATORIAL CHEMICAL SYNTHESIS

Combinational synthesis methods obtain a plurality of compositions having materially different characteristics using an apparatus having a plurality of collectors. A first quantity of fluid reactants are reacted to form a first quantity of production composition. Following completion of the collection of the first quantity of product composition, a second quantity of fluid reactants are reacted to form a second quantity of product composition materially different from the first quantity of product composition. An apparatus includes a nozzle (130) connected to a reactant source (120) and a plurality of collectors (236). The nozzle and plurality of collectors move relative to each other such that a collector can be selectively placed to receive a fluid stream emanating from the nozzle. The plurality of product compositions can be evaluated to determine their suitability for various applications.

AUTOMATED OLIGOMER SYNTHESIS

Systems, including apparatus and methods, for automated synthesis of oligomers.

Cartridge for a device for carrying out chemical or biological reactions

Multi-well rotary synthesizer

Apparatus for effecting sequential chemical syntheses

Apparatus for effecting sequential chemical synthesis of a plurality of products on solid supports. A reactor is provided having a movable rotor section including a plurality of reactor modules each containing a solid support. A top stationary stator is provided having a plurality of reactor inlets and a bottom stator is provided having a plurality of reactor outlets. The rotor is rotatably mounted between the top and bottom stators and its position is controlled to effect reactant flow to one or a plurality of desired reactor modules. Alternatively, the movable rotor section can be replaced with a linear array of reactor modules that can move linearly relative to a linear stationary stator section.

Device for carrying out multiple chemical reactions and processes in high frequency fields comprises magazine-like sample rotation body arranged in high frequency chamber

A device for carrying out multiple chemical reactions and processes in high frequency fields comprises a magazine-like sample rotation body (7) arranged in the high frequency chamber which deviates from the horizontal to receive liquid- and gas-tight closed reactor blocks. Preferred Features: The reactor blocks are formed as sample vessel plates with individual or common closable sample vessels (21). The sample vessel plates comprise a carrier plate (19) charged with glass, quartz or plastic. The carrier plate is formed by a perforated plate whose holes are arranged to receive vessel inserts in a grid, preferably 8 x 12.

Fiber array for contacting chemical species and methods for using and making same

Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays

MULTI-WELL ROTARY SYNTHESIZER

Appareil (100) permettant de synthétiser des chaînes polymères, qui comporte un dispositif (480) de commande, une pluralité de flacons (160, 181) installés avec précision et disposés de manière circulaire en bancs (115, 125, 180) multiples sur une cartouche (170, 300), un drain (740, 750, 760, 770) correspondant à chaque banc de flacons, une chambre de collecte (400), une pluralité de vannes (110, 120, 130, 470) permettant de d'introduire des réactifs dans des flacons (160, 181) sélectionnés et un système (430, 530) de tube d'évacuation destiné à évacuer la matière des flacons. Une opération de purge peut être sélectivement appliquée sur un ou plusieurs des bancs de flacons. Les bancs multiples de vannes fournissent un choix supplémentaire de réactifs en fonctionnement dans le mode série et une répartition plus rapide des réactifs en fonctionnement dans le mode parallèle. La pluralité de flacons est stockée dans la cartouche et est divisée en bancs séparés, chaque banc de flacons possédant ...

OPTICAL FIBER BUNDLE FOR DETECTING BINDING OF CHEMICAL SPECIES

The system includes a bundle of elongate optical fibers, multiple probes, a well, a light source and a detector. The optical fibers each have a first end remote from a second end, Each of the multiple probes is attached to one of the optical fibers within a predetermined section between each of the optical fiber’s first and second ends. The well is configured to hold a solution comprising a target and to receive at least the predetermined section of each of the optical fibers. The light source is configured to direct light into the first end of each of the optical fibers. Finally, the detector is configured to detect light emitted by the binding of the target to at least one of the multiple probes. In some embodiments, there are multiple bundles and multiple wells.

Oligonucleotide synthesizer

An apparatus for high-throughput combinatorial syntheses of organic molecules including a reaction vessel for containing a combinatorial-chemistry synthetic reaction, a liquid dispenser for dispensing the liquid, a liquid aspirator and an adjustment mechanism. The reaction vessel includes an ingress aperture allowing a liquid to enter into an interior of the vessel and an egress aperture for aspirating the liquid from the vessel. The liquid dispenser dispenses liquid through the ingress aperture. The liquid aspirator aspirates liquid through the egress aperture and includes a rotor for carrying the vessel and orbiting the vessel about an axis of rotation. The rotor is oriented generally in a horizontal plane and includes an adjustment mechanism for adjusting the angle of the vessel relative to the horizontal plane in response to the centrifugal force generated by orbiting the vessel about the axis of rotation. A method of combinatorial synthesis of organic molecules is also disclosed.

Parallel synthesis of multiple oligonucleotides comprises using plug-in modules rotating between stations for performing different operations

Parallel synthesis of at least 4n oligonucleotides, using plug-in modules rotating between stations, is new. Parallel synthesis of at least 4n oligonucleotides, using plug-in modules rotating between stations, is new. The method comprises: (a) arranging at least four plug-in modules, each with n reaction vessels containing a starter base coupled to an inert support or a universal support, on a turntable so that: (i) one module is at a station for removing protecting groups in all n vessels; (ii) one module is at a station for performing a washing operation in all n vessels; (iii) one module is at a station for performing a coupling operation to attach individual nucleotides in all n vessels; and (iv) one module is at a station for sequentially performing washing, capping, oxidation and washing operations in all n vessels; and (b) rotating the modules through the four stations one at a time, or rotating the stations relative to the modules, so that the indicated operations are performed ...

MULTI CHAMBER REACTOR

Multi-well rotary synthesizer

An apparatus for synthesizing polymer chains includes a controller, a plurality of precision fit vials circularly arranged in multiple banks on a cartridge, a drain corresponding to each bank of vials, a chamber bowl, a plurality of valves for delivering reagents to selective vials, and a waste tube system for purging material from the vials. A purging operation can be selectively performed on one or more of the banks of vials. The plurality of vials are stored in the cartridge and are divided among individual banks wherein each bank of vials has a corresponding drain. There is at least one waste tube system for expelling the reagent solution from vials within a particular bank of vials when the waste tube system is coupled to the corresponding drain. The cartridge holding the plurality of vials rotates relative to the stationary banks of valves and the waste tube system.

Apparatus and method for separation of liquid phases of different density and for fluorous phase organic syntheses

A simple, efficient apparatus and method for separating layers of immiscible or partially miscible liquids useful in methods of high-throughput combinatorial organic synthesis or parallel extraction of large libraries or megaarrays of organic compounds is disclosed. The apparatus and method are useful, whether as part of an automated, robotic or manual system for combinatorial organic synthesis or purification (extraction). In a preferred embodiment, an apparatus and method for separating layers of immiscible or partially miscible liquids compatible with microtiter plate type array(s) of reaction vessels is disclosed. Another application of centrifugation based liquid removal was found for washing the plates in biological assays or synthesis on modified substrates.

MECHANICAL DEVICE FOR GENERATING COMBINATORIAL LIBRARY

The present invention provides a rotating microfluidic device capable of combining portions of various liquids into a single droplet, thus generating millions of droplets with differing compositions. 1. A microfluidic device comprisinga platform with an axle member extending therefrom;a slot wheel engaged to the axle member and capable of rotation relative to the platform, the slot wheel having a substantially flat, circular upper surface;a first ring member supported by the axle member and disposed about the slot wheel, the first ring member comprising at least one well open at the top and having an open dispensing port at the bottom; anda shuttle engaged with the slot wheel such that rotation of the slot while converts to linear motion of the shuttle, wherein the shuttle comprises a collection port and the linear motion of the shuttle aligns the collection port with the dispensing port causing liquid to flow from the well into the collection port.2. The device of claim 1 , wherein the first ring member is configured to rotate relative to the platform and the slot wheel.3. The device of claim 2 , further comprising a second ring claim 2 , wherein the second ring is concentric with the first ring.4. The device of claim 3 , wherein the shuttle comprises an elongated body having a distal portion and proximate portion claim 3 , wherein the proximate portion comprises a pin.5. The device of claim 4 , wherein the flat claim 4 , circular upper surface of the slot wheel contains at least one curved groove to receive the pin.6. The device of claim 5 , wherein the pin is configured to slide within the curved groove of the slot wheel.7. The device of claim 6 , further comprising at least one motor to drive rotation of the slot wheel.8. The device of claim 7 , wherein the curved grooves are configured to convert the rotation of the slot wheel to the linear motion of the shuttle.9. The device of claim 5 , wherein the dispensing port of the well bottom has an opening that is ...

DEVICE FOR PARALLEL OLIGOMER SYNTHESIS, METHOD OF PARALLEL OLIGOMER SYNTHESIS AND USE THEREOF

A device for parallel oligomer synthesis having a centrifuge with a plurality of reactor holders configured to retain reactors at an angle and a plurality of siphon based outflow holders are disclosed. A method of parallel solid-based peptide synthesis following the timing protocol of the device and a use of the device for parallel oligomer synthesis are also disclosed. 1. A device for parallel oligomer synthesis , the device comprising:a centrifuge, the centrifuge comprisinga plurality of reactor holders, the reactor holders being configured to retain reactors at an angle, anda plurality of holders for siphon based outflows,a plurality of reactors, the reactors having upper ends and lower ends, the lower ends comprising nozzles, the reactors being positioned at an angle in the reactor holders,a plurality of siphon based outflows, the siphon based outflows having first ends and second ends, the first ends being connected to the nozzles of the lower ends of reactors, and the siphon based outflows being configured to work on a siphoning principle,a distribution rotor, the distribution rotor being provided with pre-activation compartments at its outer circumference, the pre-activation compartments being provided with grooves directed towards the outer edge of the distribution rotor, and configured to enable the transfer of contents from the pre-activation compartments of the distribution rotor to the reactors upon rotation of the distribution rotor,a lifting device, the lifting device being configured to enable the distribution rotor to move in vertical direction between an upper position and a lower position,a positioning device, the positioning device being configured to enable a rotation of the distribution rotor in the upper position independently from the centrifuge,a driving motor, the driving motor, the centrifuge and the distribution rotor having the same common axis, the driving motor being configured to enable a synchronized rotation of the centrifuge and the ...

Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays

The present invention describes a method for identifying one or more of a plurality of sequences differing by one or more single base changes, insertions, deletions, or translocations in a plurality of target nucleotide sequences. The ligation phase utilizes a ligation detection reaction between one oligonucleotide probe which has a target sequence-specific portion and an addressable array-specific portion, and a second oligonucleotide probe, having a target sequence specific portion and a detectable label. After the ligation phase, the capture phase is carried out by hybridizing the ligated oligonucleotide probes to a solid support with an array of immobilized capture oligonucleotides at least some of which are complementary to the addressable array-specific portion. Following completion of the capture phase, a detection phase is carried out to detect the labels of ligated oligonucleotide probes hybridized to the solid support. The ligation phase can be preceded by an amplification process. The present invention also relates to a kit for practicing this method, a method of forming arrays on solid supports, and the supports themselves.

Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays

The present invention describes a method for identifying one or more of a plurality of sequences differing by one or more single base changes, insertions, deletions, or translocations in a plurality of target nucleotide sequences. The ligation phase utilizes a ligation detection reaction between one oligonucleotide probe, which has a target sequence-specific portion and an addressable array-specific portion, and a second oligonucleotide probe, having a target sequence-specific portion and a detectable label. After the ligation phase, the capture phase is carried out by hybridizing the ligated oligonucleotide probes to a solid support with an array of immobilized capture oligonucleotides at least some of which are complementary to the addressable array-specific portion. Following completion of the capture phase, a detection phase is carried out to detect the labels of ligated oligonucleotide probes hybridized to the solid support. The ligation phase can be preceded by an amplification process. The present invention also relates to a kit for practicing this method, a method of forming arrays on solid supports, and the supports themselves.

Biological chip hybrid cleaning device

本发明涉及一种生物芯片杂交与清洗装置，其特征在于：它包括一箱体，在所述箱体内设置有一将其分隔为杂交腔和动力控制腔的隔板，所述杂交腔内设置有杂交装置或清洗装置；所述杂交腔下方设置有与其连通的加热腔，所述加热腔内设置有加热体，所述加热腔通过风扇和管道与所述杂交腔形成一封闭的热风循环回路，所述动力控制腔内设置有一平移装置，在所述平移装置的顶部设置一摆动装置，所述摆动装置顶部穿过所述隔板连接所述杂交腔内的杂交装置或清洗装置。本发明在空气浴控温环境中的三维摇摆运动及停止摆平的结构方式，可在生物芯片杂交过程中提高杂交的均一性。本发明可以广泛用于各种生物芯片的杂交和清洗的操作过程中。

Combinatorial chemical synthesis

조합 합성 방법은 복수의 수집기를 갖춘 장치를 이용하여 재료적으로 상이한 특성을 갖는 복수의 조성물을 얻는다. 제1 정량의 생성 조성물을 형성하도록 제1 정량의 유체 반응물을 반응시킨다. 제1 정량의 생성 조성물의 수집이 완료된 후에, 상기 제1 정량의 생성 조성물과 재료적으로 상이한 제2 정량의 생성 조성물을 형성하도록 제2 정량의 유체 반응물을 반응시킨다. 장치는 반응물 공급원(120)에 연결된 노즐(130)과 복수의 수집기(236)를 구비한다. 노즐과 복수의 수집기는, 수집기가 노즐로부터 방출되는 유체 스트림(fluid stream)을 수용하게 선택적으로 배치될 수 있도록 서로에 대해 이동한다. 복수의 생성 조성물은 다양한 용례에 대한 그들의 적합성을 결정하도록 평가될 수 있다. The combinatorial synthesis method uses a device equipped with a plurality of collectors to obtain a plurality of compositions having materially different properties. The first quantity of fluid reactant is reacted to form a first quantity of product composition. After collection of the first quantity of product composition is completed, the second quantity of fluid reactant is reacted to form a second amount of product composition that is materially different from the first amount of product composition. The apparatus has a nozzle 130 and a plurality of collectors 236 connected to the reactant source 120. The nozzle and the plurality of collectors move relative to each other such that the collector can be selectively arranged to receive a fluid stream exiting the nozzle. Multiple product compositions can be evaluated to determine their suitability for various applications.

Device for the continuous testing of materials

The present invention relates to a device for the continuous testing of at least two building blocks, which are part of a combinatorial material library. Thereby, said device is in particular characterized in that it comprises at least the following constituent parts: (i) at least one spatially stationary component with at least one means for supply, (ii) at least one spatially non-stationary component as well as (iii) at least one unit for the uptake of a building block. Thereby, at least one building block moves spatially relative to the at least one other building block during the testing.

Methods and apparatus for performing assays

An apparatus for the chemical synthesis of oligonucleotides

Reaction apparatus for chemical and especially biochemical reactions, e.g. oligonucleotide synthesis, includes sliding control-, reaction-, and chemical rotors, enabling high throughput of reactions

Biochemical and chemical reaction apparatus, comprises a reaction slider (2) including passages serving as reaction chambers (3). A selection slider (9) on one side of the reaction slider, includes a single control opening (15), which slides relative to the reaction slider. A chemical slider (17) on the opposite side of the reaction slider, includes chemical supply openings (18). Biochemical and chemical reaction apparatus, comprises a reaction slider (2) including passages serving as reaction chambers (3). A selection slider (9) on one side of the reaction slider, includes a single control opening (15), which slides relative to the reaction slider. A chemical slider (17) on the opposite side of the reaction slider, includes chemical supply openings (18). The components are arranged circularly and movement enables alignment, and dispensing of predetermined chemicals into chambers. An Independent claim is included for a method of operation in which sliders are brought to a set relative position under an applied force, which is reduced for movement.

Detection of nucleic acid sequence differences using a ligase detection reaction with a positionable array

Method and apparatus for conducting an assay

The present invention relates to methods and apparatus for conducting an assay. In particular, the present invention relates to a rotatable platform which can be used for conducting an assay, in particular multi-step assays. The present invention provides a rotatable platform adapted to immobilise a first binding partner in one or more discrete areas on a surface of said platform, or to selectively immobilise a second binding partner in one or more discrete areas on a surface of said platform. The invention also relates to methods, apparatus, a kit and the use of the rotatable platform for conducting an assay. In particular, the invention has been developed primarily for use in sequencing nucleic acid by pyrosequencing, however the invention is not limited to this field.

Method and apparatus for oligo peptide synthesis

An oligo peptide synthesis apparatus comprises a stationary apparatus part, which is stationary in relation to the frame and a movable apparatus part. The movable apparatus part comprises an oligo peptide synthesis reaction holder, which comprises a number of receptors for receiving a corresponding number of vessels and the receptors are located on a circle. The movable apparatus part comprises a revolveable holder, which has a disengageable coupling for connecting the revolveable holder to the oligo peptide synthesis reaction holder.

Fiber arrays for contacting chemical species and methods of using and making fiber arrays

An apparatus and method are provided for contacting at least two chemical species, comprising a support plate having a channel for receiving a mobile chemical species and a fiber, having a second chemical species immobilized thereon, disposed on the support plate. At least a portion of the fiber is exposed to the channel such that the mobile chemical species is capable of contacting the second chemical species. An apparatus and method for reading the fiber array, an apparatus and method for making the fiber array, and methods of using the fiber array of the present invention are also provided.

Combinatorial chemical synthesis

Method and device for the continuous testing of materials

Multi-well rotary synthesizer

An apparatus for synthesizing polymer chains includes a controller, a plurality of precision fit vials circularly arranged in multiple banks on a cartridge, a drain corresponding to each bank of vials, a chamber bowl, a plurality of valves for delivering reagents to selective vials, and a waste tube system for purging material from the vials. A purging operation can be selectively performed on one or more of the banks of vials. The plurality of vials are stored in the cartridge and are divided among individual banks wherein each bank of vials has a corresponding drain. There is at least one waste tube system for expelling the reagent solution from vials within a particular bank of vials when the waste tube system is coupled to the corresponding drain. The cartridge holding the plurality of vials rotates relative to the stationary banks of valves and the waste tube system.

One kind revolution rotation high gravity rotating packed bed

本发明公开一种公转自转超重力旋转填充床，包括：进料系统、公转系统、自转系统和出料系统；本发明将填料区转动自由度增加一级，改为公转和自转并存，这样可以实现让超重力的方向随时间快速变动，效果其一有效拓展填料对液相径向分散传递，增强分散传质作用；其二增加液相在填料中的总历程，增强综合传质效果。

Device for the continuous testing of materials

Die vorliegende Erfindung betrifft eine Vorrichtung zum kontinuierlichen Testen von mindestens zwei Bausteinen, die Teil einer kombinatorischen Materialbibliothek sind. Dabei ist diese Vorrichtung insbesondere dadurch gekennzeichnet, dass sie mindestens die folgenden Bestandteile aufweist: (i) mindestens ein räumlich feststehendes Bauteil mit mindestens einem Mittel zur Zufuhr, (ii) mindestens ein räumlich nicht feststehendes Bauteil sowie (iii) mindestens eine Einheit zur Aufnahme eines Bausteins. Dabei bewegt sich während der Testung mindestens ein Baustein räumlich relativ zu dem mindestens einen anderen Baustein. The present invention relates to a device for the continuous testing of at least two building blocks which are part of a combinatorial material library. This device is characterized in particular in that it has at least the following components: (i) at least one spatially fixed component with at least one means for supplying, (ii) at least one spatially non-fixed component and (iii) at least one unit for receiving a block. At least one building block moves spatially relative to the at least one other building block during testing.

Method and device for the continuous testing of materials

Die vorliegende Erfindung betrifft ein Verfahren zur Testung von Bausteinen, die gleich oder verschieden voneinander sind, einer Substanzbibliothek, umfassend mindestens zwei Bausteine, mit Performance-Eigenschaften, welches eine Sequenz nachfolgender Schritte aufweist: DOLLAR A (4) Testung mindestens eines Bibliotheksbausteines auf mindestens eine Performance-Eigenschaft; DOLLAR A (5) Erfassen mindestens einer Messgröße, der mindestens eine Performance-Eigenschaft des mindestens einen Bibliotheksbausteines zuzuordnen ist, durch wenigstens einen Sensor, DOLLAR A wobei wenigstens einer der Schritte (4) und (5) kontinuierlich durchgeführt wird. The present invention relates to a method for testing building blocks that are the same or different from one another, a substance library comprising at least two building blocks with performance properties, which has a sequence of the following steps: DOLLAR A (4) testing of at least one library building block for at least one performance property; DOLLAR A (5) Detection of at least one measurement variable to which at least one performance property of the at least one library module is to be assigned by at least one sensor, DOLLAR A, at least one of steps (4) and (5) being carried out continuously.

Automated chemical synthesizer and method for synthesis using same

An automated synthesizer is disclosed in which the reaction wells are moved by a carrier into alignment with stationary fluid delivery stations that are in communication with a source of reactants and/or wash solutions. In an alternate embodiment the delivery stations are moved into alignment with stationary reaction wells. A method for synthesizing chemical compounds employing the synthesizer is disclosed.

Combinatorial chemical synthesis

Combinatorial synthesis methods obtain a plurality of compositions having materially different characteristics using an apparatus having a plurality of collectors. A first quantity of fluid reactants are reacted to form a first quantity of product composition. Following completion of the collection of the first quantity of product composition, a second quantity of fluid reactants are reacted to form a second quantity of product composition, the second quantity of product composition being material different from the first quantity of product composition. An apparatus includes a nozzle connected to a reactant source and a plurality of collectors. The nozzle and plurality of collectors move relative to each other such that a collector can be selectively placed to receive a fluid stream emanating from the nozzle. The plurality of product compositions can be evaluated to determine their suitability for various applications.

Multi-channel peptide synthesizer and operating method thereof

Disclosed is a multi-channel peptide synthesizer, including a gas-bath thermotank, a plurality of reactor tubes, a motor, a rotating rack, a liquid-feeding tube, a feeding device, a vacuum tube and a nitrogen tube. The gas-bath thermotank body provides a desired constant temperature for reaction. The reactor tube provides a place for peptide synthesis and resin washing. The motor and the rotating rack are used to fully mix the reaction and cleaning solutions. Various liquid reagents required are fed to the reactor tube through the liquid-adding tube. Various materials required are prepared in advance in the feeding device and directly fed to the reactor tube. The reaction or washing solution in the reactor tube is pumped to a waste liquid tank through the vacuum tube. Nitrogen is introduced into each reactor tube through the nitrogen tube. This device can be applied in batch-wise peptide synthesis using solid-phase methods.

Device for parallel oligomer synthesis, method of parallel oligomer synthesis and use thereof

Automated chemical synthesizer and method for synthesis using same

An automated synthesizer is disclosed in which the reaction wells are moved by a carrier into alignment with stationary fluid delivery stations that are in communication with a source of reactants and/or wash solutions. In an alternate embodiment the delivery stations are moved into alignment with stationary reaction wells. A method for synthesizing chemical compounds employing the synthesizer is disclosed.

Automated peptide synthesizer

A device capable of synthesizing a plurality of selected peptides by automatically mixing various amino acids, solvents, and activators and adding these to resins contained in a plurality of individual reaction vessels. A plurality of amino acids are contained in vessels within a carousel which is rotated into position where a needle is inserted into a selected vessel to transport the amino acid to a pre-reaction vessel for mixing with other selected amino acids which were previously drawn from the carousel. The mixture of amino acids is then transported to a reaction vessel containing the resin balls for growth of the selected peptide. A computer controls valves and pumps and is programmed to mix selected elements, apply the mixture to resins and grow peptides. A preferred method reduces cycle time and saves solvent by using the activator delivery step to simultaneously clean the needle of amino acid residue.

Continuous polymer synthesizer

Described is a system and method for synthesizing polymeric molecules such as oligonucleotides and polypeptides. The system is capable of continuously synthesizing molecules by providing an array of reaction sites and an array of stations for carrying out synthetic manipulations. The reaction sites in the former array can be placed in a fixed order and at fixed intervals relative to each other. Similarly, the stations can be placed in a fixed order and at fixed intervals relative to each other. The two arrays can be moved relative to each other such that the stations carry out desired steps of a reaction scheme at each reaction site. The relative locations of the stations and the schedule for the relative movement can correlate with the order and duration of reaction steps in the reaction scheme such that once a reaction site has completed a cycle of interacting with the full array of stations then the reaction scheme is complete.

METHOD AND DEVICE FOR THE PARALLEL PRODUCTION OF OLIGONUCLEOTIDES

Method and apparatus for conducting an assay

本发明涉及用于实施测定法的方法和装置。尤其，本发明涉及可以用于实施测定法、尤其多步骤测定法的可转动平台。本发明提供一种可转动平台，其适应于在所述平台的表面上的一个或多个分立区域内固定第一结合配偶体，或适应于在所述平台的表面上的一个或多个分立区域内选择性固定第二结合配偶体。本发明也涉及用于实施测定法的方法、装置、试剂盒和可转动平台用途。尤其，已经主要开发本发明用于通过焦磷酸测序法对核酸测序，然而本发明不限于该领域。

Device for washing and hybridization of biochips

A device for biochip hybridization or washing is provided, which comprises a carousel ( 12 ), a translational movement controller, a revolving movement controller, and optionally a heating chamber ( 13 ). The revolving movement controller controls the revolving movement of the carousel and allows it to move in a wobbling fashion, allowing liquid movement of the hybridization or washing boxes ( 11 ) during hybridization and/or washing of biochips on the carousel. The translational movement controller brings the carousel back to horizontal position once the revolving movement controller stops, thereby ensures that the liquid do not spill out. The heating chamber circulates hot air within the device, thereby ensures that the hybridization and washing in a thermostatic condition.

Thermocycler reaction control

Provided herein are devices, methods, and systems for polynucleotide synthesis comprising a thermocycler comprising a plurality of individual chambers having a capability to control its own temperature setting and a machine learning for generating a recommendation of a design of experiment for polynucleotide synthesis.

Process and device for the parallel preparation of at least 4n oligonucleotides

In a process and a device for the parallel preparation of at least 4n oligonucleotides, at least four inserts each with n reaction vessels are first arranged on a plate ( 16 ), each reaction vessel containing a nucleotide initiator base bound to an inert carrier. Particular operations are then carried out in parallel with one another at four stations ( 28, 30, 32, 34 ), and in particular a deblocking operation simultaneously in all n reaction vessels of the insert at the first station ( 28 ), a first washing operation simultaneously in all n reaction vessels of the insert at the second station ( 30 ), a coupling operation in all n reaction vessels of the insert at the third station ( 32 ), and, simultaneously in all n reaction vessels of the insert at the fourth station ( 34 ), a second washing operation followed by a capping operation followed by a third washing operation followed by an oxidation operation followed by a fourth washing operation. The plate ( 16 ) with the inserts is rotated station by station, the abovementioned operations being carried out, until the desired oligonucleotides have been formed by coupling individual nucleotides to one another.

并行多步生物反应系统和方法

一种并行多步生物反应系统(10)，其包括：(a)基底装置(12)，其包括配置成保持多个生物反应基底(20)的多个生物反应基底保持器(18)；(b)孔装置(14)，其包括多个流体孔(22)，所述流体孔(22)对应于多步生物反应的多个步骤；(c)致动器(16)，其被配置成：(i)相对于基底装置(12)或孔装置(14)移动基底装置(12)或孔装置(14)中的另一个，以改变作为一组的生物反应基底(20)相对于作为一组的流体孔(22)的对准；以及(ii)使生物反应基底(20)与流体孔(22)中的流体接触和脱离接触。

Syntheseeinrichtung für oligonukleotide unter nutzung von fliehkraft

Systems and approaches for drug processing

A drug processing system includes a workstation, at least one deck module movably positionable within the workstation, at least one filter plate operably coupled with the at least one deck module, an agitating member, and a liquid handler member. The at least one filter plate has a plurality of wells to receive a fluid therein. The agitating member is adapted to move the at least one filter plate according to an agitation system. The liquid handler member is adapted to selectively add a fluid to at least one of the plurality of wells and/or remove a fluid from the at least one of the plurality of wells according to a liquid handling system. The agitating member is adapted to move the at least one filter plate while the liquid handler member selectively adds and/or removes the fluid from the at least one of the plurality of wells.

Apparatus and method for separation of liquid phases of different density and for fluorous phase organic syntheses

A simple, efficient apparatus and method for separating layers of immiscible or partially miscible liquids useful in methods of high-throughput combinatorial organic synthesis or parallel extraction of large libraries or megaarrays of organic compounds is disclosed. The apparatus and method are useful, whether as part of an automated, robotic or manual system for combinatorial organic synthesis or purification (extraction). In a preferred embodiment, an apparatus and method for separating layers of immiscible or partially miscible liquids compatible with microtiter plate type array(s) of reaction vessels is disclosed. Another application of centrifugation based liquid removal was found for washing the plates in biological assays or synthesis on modified substrates.

Multi-station reaction apparatus

In a multi-station reaction apparatus ( 1 ) comprising an annular heat transfer block ( 30 ) formed with a plurality of cavities ( 31 ) for receiving respective reaction vessels ( 36 ), the upper surface of the heat transfer block ( 30 ) has a central recess ( 32 ) around which the said plurality of cavities ( 31 ) are arrayed. The apparatus further comprises a removable cooling module ( 4 ), the central recess ( 32 ) of the heat transfer block ( 30 ) being shaped so as to accommodate the removable cooling module ( 4 ) and corresponding surfaces of the central recess ( 32 ) of the heat transfer block ( 30 ) and the cooling module ( 4 ) being shaped such that there is a stepped interface between them. The cooling module ( 4 ) comprises a metal block having channels therein through which coolant circulates when the cooling module ( 4 ) is in use. The apparatus further comprises refluxing means ( 5 ) which are positioned above the heat transfer block ( 30 ) and arrayed annularly around a central hole ( 52 ), whereby the cooling module ( 4 ) can be inserted or removed from the heat, transfer block ( 30 ) through the hole ( 52 ) without disturbance or removal of the said reaction vessels ( 36 ) or refluxing means ( 5 ). In an alternative embodiment, the cooling module may be annular with a central recess for receiving the heat transfer block.

Multi-station reaction apparatus

In a multi-station reaction apparatus (1) comprising an annular heat transfer block (30) formed with a plurality of cavities (31) for receiving respective reaction vessels (36), the upper surface of the heat transfer block (30) has a central recess (32) around which the said plurality of cavities (31) are arrayed. The apparatus further comprises a removable cooling module (4), the central recess (32) of the heat transfer block (30) being shaped so as to accommodate the removable cooling module (4) and corresponding surfaces of the central recess (32) of the heat transfer block (30) and the cooling module (4) being shaped such that there is a stepped interface between them. The cooling module (4) comprises a metal block having channels therein through which coolant circulates when the cooling module (4) is in use. The apparatus further comprises refluxing means (5) which are positioned above the heat transfer block (30) and arrayed annularly around a central hole (52), whereby the cooling module (4) can be inserted or removed from the heat, transfer block (30) through the hole (52) without disturbance or removal of the said reaction vessels (36) or refluxing means (5). In an alternative embodiment, the cooling module may be annular with a central recess for receiving the heat transfer block.

Multi-station reaction apparatus

In a multi-station reaction apparatus (1) comprising an annular heat transfer block (30) formed with a plurality of cavities (31) for receiving respective reaction vessels (36), the upper surface of the heat transfer block (30) has a central recess (32) around which the said plurality of cavities (31) are arrayed. The apparatus further comprises a removable cooling module (4), the central recess (32) of the heat transfer block (30) being shaped so as to accommodate the removable cooling module (4) and corresponding surfaces of the central recess (32) of the heat transfer block (30) and the cooling module (4) being shaped such that there is a stepped interface between them. The cooling module (4) comprises a metal block having channels therein through which coolant circulates when the cooling module (4) is in use. The apparatus further comprises refluxing means (5) which are positioned above the heat transfer block (30) and arrayed annularly around a central hole (52), whereby the cooling module (4) can be inserted or removed from the heat, transfer block (30) through the hole (52) without disturbance or removal of the said reaction vessels (36) or refluxing means (5). In an alternative embodiment, the cooling module may be annular with a central recess for receiving the heat transfer block.

Fasermatrix zum zusammenbringen von chemischen stoffen, sowie verfahren zur herstellung und anwendung davon

Oligonucleotide synthesizer using centrifugal force

一种多通道多肽合成反应装置及其操作方法

本发明公开了一种多通道多肽合成反应装置，包括气浴恒温箱体、合成反应管、电机和旋转架、加液管道、加料装置、真空和氮气管道；所述气浴恒温箱体提供反应所需恒定的温度；合成反应管提供多肽合成反应、树脂清洗的场所；电机和旋转架用于充分混匀反应液和清洗液；加液管道将所需的各种液体试剂通过管道加入到合成反应管内；加料装置中预先备好反应所需的各种物料，需加料时直接将物料加入到合成反应管内；真空管道可将合成反应管内的反应液或清洗液抽到废液缸内；氮气管道可将氮气通入各个合成反应管中。该多功能多肽合成反应装置可广泛应用于固相合成法批量的合成多肽，具有可多通道连续批量生产、可控温、反应充分、加料方便等优点。

Apparatus and method for concurrent chemical synthesis

This invention provides an apparatus for preparing chemical libraries. The apparatus includes: (1) a carousel comprising a plurality of reaction mounts having at least one reaction well; (2) a rotator that rotates the carousel step-wise; (3) a fluid delivery system; (4) a drain system; and (5) a programmable computer that controls the operation of the apparatus, including the rotator, the fluid delivery system, the drain system and other systems in the apparatus. The preparation of chemical libraries involves rotating the carousel through a plurality of stations. At each station, a physical step in a reaction protocol is carried out on the reaction wells of the mount docked at the station.

Apparatus and method for concurrent chemical synthesis

This invention provides an apparatus for preparing chemical libraries. The apparatus includes: (1) a carousel comprising a plurality of reaction mounts having at least one reaction well; (2) a rotator that rotates the carousel step-wise; (3) a fluid delivery system; (4) a drain system; and (5) a programmable computer that controls the operation of the apparatus, including the rotator, the fluid delivery system, the drain system and other systems in the apparatus. The preparation of chemical libraries involves rotating the carousel through a plurality of stations. At each station, a physical step in a reaction protocol is carried out on the reaction wells of the mount docked at the station.

Apparatus and method for concurrent chemical synthesis

This invention provides an apparatus for preparing chemical libraries. The apparatus includes: (1) a carousel comprising a plurality of reaction mounts having at least one reaction well; (2) a rotator that rotates the carousel step-wise; (3) a fluid delivery system; (4) a drain system; and (5) a programmable computer that controls the operation of the apparatus, including the rotator, the fluid delivery system, the drain system and other systems in the apparatus. The preparation of chemical libraries involves rotating the carousel through a plurality of stations. At each station, a physical step in a reaction protocol is carried out on the reaction wells of the mount docked at the station.

Systems and approaches for drug processing

A drug processing system includes a workstation, at least one deck module movably positionable within the workstation, at least one filter plate operably coupled with the at least one deck module, an agitating member, and a liquid handler member. The at least one filter plate has a plurality of wells to receive a fluid therein. The agitating member is adapted to move the at least one filter plate according to an agitation system. The liquid handler member is adapted to selectively add a fluid to at least one of the plurality of wells and/or remove a fluid from the at least one of the plurality of wells according to a liquid handling system. The agitating member is adapted to move the at least one filter plate while the liquid handler member selectively adds and/or removes the fluid from the at least one of the plurality of wells.

用于药物加工的系统和方法

一种药物加工系统，包括工作站、可移动地定位在该工作站内的至少一个台板模块、与该至少一个台板模块可操作地联接的至少一个过滤板、搅拌构件、和液体操作构件。该至少一个过滤板具有多个孔以将流体接收在其中。该搅拌构件适于根据搅拌系统来移动该至少一个过滤板。该液体操作构件适于根据液体操作系统来选择性地将流体添加至该多个孔中的至少一个孔和/或从该多个孔中的至少一个孔中移除流体。该搅拌构件适于在该液体操作构件选择性地对该多个孔中的至少一个孔添加和/或移除流体的同时移动该至少一个过滤板。

Parallel multi-step bio-reaction system and method

A parallel multi-step bio-reaction system(10) comprising: (a) a substrate arrangement(12) comprising a plurality of bio-reaction substrate holders(18) configured to hold a plurality of bio-reaction substrates(20); (b) a well arrangement(14) comprising a plurality of fluidic wells(22), the fluidic wells(22) corresponding to a plurality of steps of a multi-step bio-reaction; (c) an actuator(16) configured to: (i) move either the substrate arrangement(12) or the well arrangement(14) relative to the other of the substrate arrangement(12) or the well arrangement(14) to change the alignment of the bio-reaction substrates(20) as a group relative to the fluidic wells(22) as a group; and (ii) bring the bio-reaction substrates(20) into and out of contact with fluids in the fluidic wells(22).

Verfahren und vorrichtung zur parallelen herstellung von oligonukleotiden

Reaction container and analyzing apparatus

A reaction container holds a liquid agitated by sound waves. The reaction container includes two openings which serve as an introduction/discharge port of the liquid, and a holding member which has the two openings, holds the liquid between the two openings, and includes a sound wave generator arranged on one of a side surface and a portion close to the side surface for emitting sound waves to a portion between the two openings to agitate the liquid.

Oligonucleotide synthesizer using centrifugal force

An apparatus for high-throughput combinatorial syntheses of organic molecules including a reaction vessel for containing a combinatorial-chemistry synthetic reaction, a liquid dispenser for dispensing the liquid, a liquid aspirator and an adjustment mechanism. The reaction vessel includes an ingress aperture allowing a liquid to enter into an interior of the vessel and an egress aperture for aspirating the liquid from the vessel. The liquid dispenser dispenses liquid through the ingress aperture. The liquid aspirator aspirates liquid through the egress aperture and includes a rotor for carrying the vessel and orbiting the vessel about an axis of rotation. The rotor is oriented generally in a horizontal plane and includes an adjustment mechanism for adjusting the angle of the vessel relative to the horizontal plane in response to the centrifugal force generated by orbiting the vessel about the axis of rotation. A method of combinatorial synthesis of organic molecules is also disclosed.

利用離心力的低核苷酸合成器

Dna manufacturing, storage, and access system

A system includes a synthesizer unit having a fluid input to receive fluids and a communication input to receive commands to synthesize data-encoded DNA sequences and cleave the DNA. A first flexible chemistry reaction chamber module may be fluidically coupled to the synthesizer unit to receive the data-encoded DNA sequences and amplify the sequences. A deposition unit may be fluidically coupled to the first flexible chemistry reaction chamber module to receive the amplified DNA sequences and encapsulate the amplified DNA sequences into one or more wells in a storage plate for storage and retrieval to and from a plate storage unit. Retrieved DNA may be processed and read by further units.

Dna manufacturing, storage, and access system

A system includes a synthesizer unit having a fluid input to receive fluids and a communication input to receive commands to synthesize data-encoded DNA sequences and cleave the DNA. A first flexible chemistry reaction chamber module may be fluidically coupled to the synthesizer unit to receive the data-encoded DNA sequences and amplify the sequences. A deposition unit may be fluidically coupled to the first flexible chemistry reaction chamber module to receive the amplified DNA sequences and encapsulate the amplified DNA sequences into one or more wells in a storage plate for storage and retrieval to and from a plate storage unit. Retrieved DNA may be processed and read by further units.

Reagent reaction container

A reagent reaction container includes a container body having a plurality of receiving bores through top and bottom sides thereof, a plurality of filter holders mounted in the receiving bores respectively and each having a through hole through top and bottom sides thereof and an engagement portion engaged with a periphery wall of the respective receiving bore, a plurality of drain elements mounted in the through holes of the filter holders respectively, and a plurality of filters respectively mounted in the through holes of the filter holders and supported on the respective drain element. The drain element has a crevice that is closed when the drain element receives no pressure and is opened when the drain element receives a predetermined external force.

Rotatable device containing wafers for dna processing

A system includes a synthesizer unit having a fluid input to receive fluids and a communication input to receive commands to synthesize data-encoded DNA sequences and cleave the DNA. A first flexible chemistry reaction chamber module may be fluidically coupled to the synthesizer unit to receive the data-encoded DNA sequences and amplify the sequences. A deposition unit may be fluidically coupled to the first flexible chemistry reaction chamber module to receive the amplified DNA sequences and encapsulate the amplified DNA sequences into one or more wells in a storage plate for storage and retrieval to and from a plate storage unit. Retrieved DNA may be processed and read by further units.

Dna manufacturing, storage, and access system

A system includes a synthesizer unit having a fluid input to receive fluids and a communication input to receive commands to synthesize data-encoded DNA sequences and cleave the DNA. A first flexible chemistry reaction chamber module may be fluidically coupled to the synthesizer unit to receive the data-encoded DNA sequences and amplify the sequences. A deposition unit may be fluidically coupled to the first flexible chemistry reaction chamber module to receive the amplified DNA sequences and encapsulate the amplified DNA sequences into one or more wells in a storage plate for storage and retrieval to and from a plate storage unit. Retrieved DNA may be processed and read by further units.

An oligo peptide synthesis apparatus

An oligo peptide synthesis apparatus comprises a housing and a drive motor mounted in the housing and having an output shaft. The oligo peptide synthesis apparatus further comprises an oligo peptide synthesis reaction holder defining a centre line and comprising a number of receptors for receiving a corresponding number of vessels. The receptors are located on a first circle having its centre on the centre line. The holder has a coupling for connecting the holder to the drive motor, the holder being journalled horizontally and rotatably relative to the housing for stepwise revolving around the centre line relative to the housing powered by the drive motor. The oligo peptide synthesis apparatus further comprises a number of synthesis material lines supported relatively to the housing, each of the lines communicating with a supply container and each of the lines defining a discharge end. The discharge ends are positioned on a second circle having its centre on the centre line for allowing a specific discharge end to be positioned above a specific vessel received in the holder.

Autonomous exploration for the synthesis of chemical libraries

The invention provides a method for the exploration of chemical space through a multigenerational series of synthetic stages. In a first stage, a series of reactions is performed. The products of the first series are analysed, and a first product from the series of reactions is selected. The first product is used as a chemical input for each of the reactions in a second series of reactions. The products of the second series are analysed. The first and second stages are performed autonomously. The selection of a product in the first stage comprises the comparison of the products from the first series of reactions against a fitness function, where the selected product has a superior fitness compared with one or more other products in the first series. Each reaction in the first series differs in one or more chemical and/or physical inputs. Each reaction in the second series differs in one or more chemical and/or physical inputs. The invention also provides an automated exploration apparatus for performing the method, and the apparatus comprises a controller, and a chemical synthesiser and an analytical unit which are operable by the controller, as described herein.

Verfahren und vorrichtung zur parallelen herstellung von oligonukleotiden

Bei einem Verfahren und einer Vorrichtung zur parallelen Herstellung von wenigstens 4n Oligonukleotiden werden zunächst wenigstens vier Einschübe mit je n Reaktionsgefässen auf einem Teller (16) angeordnet, wobei jedes Reaktionsgefäss eine an einen inerten Träger gebundene Nukleotid-Startbase enthält. Sodann werden an vier Stationen (28, 30, 32, 34) bestimmte Operationen parallel zueinander durchgeführt, und zwar eine Deblock-Operation gleichzeitig in allen n Reaktionsgefässen des sich an der ersten Station (28) befindenden Einschubes, eine erste Wasch-Operation gleichzeitig in allen n Reaktionsgefässen des sich an der zweiten Station (30) befindenden Einschubes, eine Coupling-Operation in allen n Reaktionsgefässen des sich an der dritten Station (32) befindenden Einschubes, und, gleichzeitig in allen n Reaktionsgefässen des sich an der vierten Station (34) befindenden Einschubes, eine zweite Wasch-Operation gefolgt von einer Capping-Operation gefolgt von einer dritten Wasch-Operation gefolgt von einer Oxidations-Operation gefolgt von einer vierten Wasch-Operation. Der Teller (16) mit den Einschüben wird stationsweise unter Ausführen der zuvor genannten Operationen solange rotiert, bis die gewünschten Oligonukleotide durch Aneinanderkoppeln einzelner Nukleotide entstanden sind.

Verfahren und vorrichtung zur parallelen herstellung von oligonukleotiden

Bei einem Verfahren und einer Vorrichtung zur parallelen Herstellung von wenigstens 4n Oligonukleotiden werden zunächst wenigstens vier Einschübe mit je n Reaktionsgefässen auf einem Teller (16) angeordnet, wobei jedes Reaktionsgefäss eine an einen inerten Träger gebundene Nukleotid-Startbase enthält. Sodann werden an vier Stationen (28, 30, 32, 34) bestimmte Operationen parallel zueinander durchgeführt, und zwar eine Deblock-Operation gleichzeitig in allen n Reaktionsgefässen des sich an der ersten Station (28) befindenden Einschubes, eine erste Wasch-Operation gleichzeitig in allen n Reaktionsgefässen des sich an der zweiten Station (30) befindenden Einschubes, eine Coupling-Operation in allen n Reaktionsgefässen des sich an der dritten Station (32) befindenden Einschubes, und, gleichzeitig in allen n Reaktionsgefässen des sich an der vierten Station (34) befindenden Einschubes, eine zweite Wasch-Operation gefolgt von einer Capping-Operation gefolgt von einer dritten Wasch-Operation gefolgt von einer Oxidations-Operation gefolgt von einer vierten Wasch-Operation. Der Teller (16) mit den Einschüben wird stationsweise unter Ausführen der zuvor genannten Operationen solange rotiert, bis die gewünschten Oligonukleotide durch Aneinanderkoppeln einzelner Nukleotide entstanden sind.

同時化学合成のための装置および方法

(57)【要約】 本発明は、化学ライブラリーを調製するための装置を提供する。この装置は、以下を備える：（１）少なくとも１つの反応ウェルを有する複数の反応台を備える回転ラック；（２）この回転ラックを段階的に回転させる回転子；（３）流体送達システム；（４）排出システム；および（５）この装置の操作を制御するプログラム可能なコンピューターであって、この装置において回転子、流体送達システム、排出システムおよび他のシステムを含む。化学ライブラリーの調製は、複数のステーションを通じて回転ラックを回転させる工程を包含する。各ステーションにて、反応プロトコルの物理的工程は、このステーションでドッキングされた台の反応ウェル上で行われる。

用于药物加工的系统和方法

一种药物加工系统，包括工作站、可移动地定位在该工作站内的至少一个台板模块、与该至少一个台板模块可操作地联接的至少一个过滤板、搅拌构件、和液体操作构件。该至少一个过滤板具有多个孔以将流体接收在其中。该搅拌构件适于根据搅拌系统来移动该至少一个过滤板。该液体操作构件适于根据液体操作系统来选择性地将流体添加至该多个孔中的至少一个孔和/或从该多个孔中的至少一个孔中移除流体。该搅拌构件适于在该液体操作构件选择性地对该多个孔中的至少一个孔添加和/或移除流体的同时移动该至少一个过滤板。

High throughput mechanical alloying and screening

The invention provides an apparatus and methods for conducting ball milling operations in a high throughput manner. Also provided are devices and methods for high throughput screening of alloys and other materials to identify those that have desired gas storage properties.

并行多步生物反应系统和方法

一种并行多步生物反应系统(10)，其包括：(a)基底装置(12)，其包括配置成保持多个生物反应基底(20)的多个生物反应基底保持器(18)；(b)孔装置(14)，其包括多个流体孔(22)，所述流体孔(22)对应于多步生物反应的多个步骤；(c)致动器(16)，其被配置成：(i)相对于基底装置(12)或孔装置(14)移动基底装置(12)或孔装置(14)中的另一个，以改变作为一组的生物反应基底(20)相对于作为一组的流体孔(22)的对准；以及(ii)使生物反应基底(20)与流体孔(22)中的流体接触和脱离接触。

High throughput mechanical alloying and screening

The invention provides an apparatus and methods for conducting ball milling operations in a high throughput manner. Also provided are devices and methods for high throughput screening of alloys and other materials to identify those that have desired gas storage properties.

Mehrfach-bioreaktorvorrichtung

Dna writer

Technologies for data storage in nucleic acids include a system for assembling an identifier nucleic acid molecule encoding digital information. The system includes a substrate removably mounted on a carrier, a writing module configured to dispense a droplet of a solution comprising a nucleic acid molecule onto the substrate, a collection module configured to collect droplets from the substrate, a cleaning module to clean the substrate after collection of the droplets; and a control system control the carrier to transfer the substrate between the writing module, the collection module, and the cleaning module.

Automated peptide production machine

A device capable of synthesizing a plurality of selected peptides by automatically mixing various amino acids, solvents, and activators and adding these to resins contained in a plurality of individual reaction vessels. A plurality of amino acids are contained in vessels within a carousel which is rotated into position where a syringe is inserted into a selected vessel to transport the amino acid within to a pre-reaction vessel for mixing with other selected amino acids which were previously drawn from the carousel. The mixture of amino acids is then transported to a reaction vessel containing the resin balls for growth of the selected peptide. The device includes a computer, controllable valves, at least one pump, pressurized gas such as nitrogen for transporting fluids, various vessels containing amino acids, solvents, activators, resins, and tubing connecting these elements. The computer is programmable to sample, mix selected components, and apply the mixture to resins for growing peptides.