14-01-2021 дата публикации
Номер: US20210009942A1
Принадлежит:
The present invention is directed generally to dry cell culture media or feeds in pellet formats which can be reconstituted into liquid media for culturing cells in vitro. Each pellet composition may comprise the same or a different composition; for example, different vitamins, amino acids, buffers, trace salts, pH, iron chelators, etc. The invention also relates to methods of making dry cell culture media by altering ratios of different pellet compositions, or, methods of making modular dry cell culture media, or customizing media formulations for growing a cell type using pellets. The media pellets may be easier to handle either before reconstitution, during shipping and handling; and/or during reconstitution. Media pellets may be used in any container like bags including sterile, single use bags for preparing media formulations. The invention also relates to kits and culture systems using media pellets. 126.-. (canceled)27. A pelletized cell culture medium , feed , supplement or additive comprising one or more amino acids , one or more binders , one or more vitamins , one or more salts , and one or more trace components , wherein the amino acids are 25 to 40 percent , the binders are 20 to 65 percent , the vitamins are 1 to 5 percent , the salts are 2 to 10 percent , and the trace components are 0.01 to 0.05 percent.28. (canceled)29. The pelletized cell culture medium claim 27 , feed claim 27 , supplement or additive of claim 27 , wherein the vitamins are selected from one or more of vitamin B12 claim 27 , biotin claim 27 , choline claim 27 , folic acid claim 27 , niacinamide claim 27 , pyridoxine claim 27 , riboflavin claim 27 , thiamine claim 27 , ascorbic acid claim 27 , and para-aminobenzoic acid (PABA).30. (canceled)31. The pelletized cell culture medium claim 27 , feed claim 27 , supplement or additive of claim 27 , wherein the salts are selected from one or more of buffer salts claim 27 , iron claim 27 , zinc claim 27 , calcium claim 27 , copper claim 27 , ...
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