СПОСОБЫ И УСТРОЙСТВА ДЛЯ ОСУЩЕСТВЛЕНИЯ МАТРИЧНЫХ МИКРОКРИСТАЛЛИЗАЦИЙ

... 1. Способ осуществления матричных микрокристаллизаций для определения подходящих условий кристаллизации для молекулы, включающий формирование матрицы микрокристаллизаций, где каждая микрокристаллизация включает каплю, содержащую раствор маточной жидкости, композиция которого варьируется в матрице, и молекулу, которая должна быть кристаллизована, причем капля имеет объем, меньший, чем 1 мкл, хранение матрицы микрокристаллизаций при условиях, пригодных для формирования молекулярных кристаллов в каплях, находящихся в этой матрице, и детектирование формирования молекулярных кристаллов в каплях. 2. Способ по п.1, где молекула представляет собой макромолекулу. 3. Способ по п.1, где молекула представляет собой белок. 4. Способ по п.1, где макромолекула имеет молекулярную массу, по меньшей мере, 500 Дальтон. 5. Способ по п.1, где капля имеет объем меньший, чем около 750 нл. 6. Способ по п.1, где капля имеет объем меньший, чем около 500 нл. 7. Способ по п.1, где капля имеет объем меньший, чем около ...

СИСТЕМЫ И СПОСОБЫ ДЛЯ СОДЕРЖАНИЯ БИОЛОГИЧЕСКИХ ОБРАЗЦОВ

... 1. Изделие для удержания множества биологических образцов, содержащее:подложку, содержащую первую поверхность и противоположную вторую поверхность, иподложку, содержащую первую поверхность и противоположную вторую поверхность, имножество реакционных центров в подложке, каждый из которых проходит от отверстия в первой поверхности до отверстия во второй поверхности, причем реакционные центры выполнены для создания достаточного поверхностного натяжения под действием капиллярных сил, чтобы удерживать соответствующие биологические образцы,при этом плотность реакционных центров по меньшей мере на части одной из поверхностей составляет по меньшей мере 170 отверстий на квадратный миллиметр.2. Изделие по п. 1, в котором по меньшей мере одна из поверхностей имеет шероховатость поверхности, характеризующуюся одной или большим количеством величин: средним арифметическим отклонением профиля (Ra), которое меньше или равно 5 нанометров, максимальной глубиной профиля впадины (Rv), которая меньше или равна ...

VORRICHTUNG ZUM THERMOSTATISIEREN UND MISCHEN DES INHALTES VON GEFAESSEN EINER MIKROTITRATIONSPLATTE

Reaktorsteuerungssystem

Anordnung und Verfahren zur mehrkanaligen Fluoreszenzmessung in PCR-Proben

Anordnung zur mehrkanaligen Fluoreszenzmessung in PCR-Proben umfassend • einen Heizblock (1) mit Aufnahme (2) für PCR-Proben aufnehmende Kavitäten (41) einer Mikrotiterplatte (4), • eine transparente Folie (9) zum Verschließen der Kavitäten (41), • einen Messkopf (3), gegen den der Heizblock (1) hebbar und absenkbar angeordnet ist, wobei sich im Messkopf (3) eine Anregungslichteinheit (A), eine Detektionseinheit (D) sowie eine mechanische Einheit (M) befinden, dadurch gekennzeichnet, • dass die Detektionseinheit (D) eine drehbare Scheibe (26) mit Strahlteilermodulen (21; 31), einen Umlenkspiegel sowie einen Detektor (30) enthält wobei die Strahlteilermodule (21; 31) Filter einen dichroitischen Strahlteiler (27) eine Linse umfassen so dass Licht von der Anregungslichteinheit (A), nachdem es einen ersten Filter passiert hat, nacheinander in eine Vielzahl auf einer Kreisbahn angeordneter Lichtleiter (7) einkoppelbar ist, über die das Licht über vor den probenseitigen Enden der Lichtleiter ...

Vorrichtung und Verfahren zur parallelen Durchführung von Experimenten

Es wird eine modulare Vorrichtung und ein Verfahren zur parallelen Durchführung von Experimenten an Prozessstoffen für die Entwicklung von technischen Verfahren beschrieben. Die modulare Vorrichtung eignet sich insbesondere zur miniaturisierten, automatisierten Durchführung komplexer Verfahren. Die Vorrichtung besteht wenigstens aus einer Vielzahl von Einzelreaktoren (1) zur Aufnahme der Prozessstoffe (10), wobei die Einzelreaktoren (1) unabhängig voneinander wenigstens bezüglich ihres inneren Drucks und ihrer Temperatur und der Drehzahl steuerbar oder regelbar sind und wobei die Einzelreaktoren (1) mindestens aufgebaut sind aus: DOLLAR A je einer druckfesten Kammer (2, 2a, 2b, 2c, 2d, 2e) mit einem separierbaren Probengefäß (12), gegebenenfalls je einer Rührvorrichtung (4), DOLLAR A je einer Heizeinrichtung (31) und/oder einer Kühleinrichtung (32) zur Temperierung des Probengefäßes (12), DOLLAR A einer Kontrolleinheit (700) zur Kontrolle oder Regelung wenigstens des Drucks und der Temperatur ...

Vorrichtung und Verfahren zum gleichzeitigen Testen der Einwirkung von Flüssigkeiten auf Flächengebilde sowie Verwendung der Vorrichtung

Die Erfindung betrifft eine Vorrichtung zum gleichzeitigen Testen der Einwirkung von Flüssigkeiten auf ein oder mehrere Flächengebilde (5), die mindestens ein zusammenhängendes Element (2) mit einer Vielzahl von Kammern, eine Abdeckung (1) zum Verschließen dieser Kammern und einen Antrieb zum Bewegen des Elements (2) umfaßt und die dadurch gekennzeichnet ist, daß eine gleichmäßige Erwärmung der Flüssigkeiten in den Kammern mit mehr als 1 DEG C/s erreicht werden kann.

Vorrichtung und Verfahren zum gleichzeitigen Testen der Einwirkung von Flüssigkeiten auf Flächengebilde sowie Verwendung der Vorrichtung

Vorrichtung zum gleichzeitigen Testen der Einwirkung von Flüssigkeiten auf ein oder mehrere Flächengebilde (5) umfassend mindestens ein zusammenhängendes Element (2) mit einer Vielzahl von Kammern, eine Abdeckung (1) zum Verschließen dieser Kammern und einen Antrieb zum Bewegen des Elements (2), wobei das Flächengebilde (5) zwischen dem Element (2) und der Abdeckung (1) im Bereich der Vertiefungen (3, 4) anzuordnen ist, dadurch gekennzeichnet, daß die Abdeckung (1) und das Element (2) aus einem mit Mikrowellenstrahlung aufheizbaren Werkstoff bestehen, so daß unter Verwendung eines Mikrowellengeräts eine gleichmäßige Erwärmung der Flüssigkeiten in den Kammern mit mehr als 1°C/s erreicht werden kann ...

Improved two-part microwell plates and methods of fabricating same

Improvements in and relating to reaction vessels for use with such vessels

A vessel

Sample carrier for dried biological samples

Reaction plate

Temperature regulating container

PCR cartridge

Sample holder for the discrete analysis of liquid preparations

A sample holder for the discrete analysis of liquid preparations which can also be used as a measuring cell, which ensures the same layer thickness of all the samples in it and which is temperable and easy to clean includes a central body (1) which contains cavities (7) and which is bounded by a lower terminal element (4) at the bottom and an upper recessed terminal element (5) at the top, these terminal elements (4, 5) having surfaces lying in respective common planes which are at least in the vicinity of the bottom and top surfaces (2, 3) of the central body (1). The central body (1) may include passages for temperature control means (Fig. 3) between the central body (1) and the power element (4) there may be a sheet carrying analysis reactants ((22). Fig. 4c not shown). ...

Microtitre plate with heating means to prevent condensation

A reaction vessel 10, typically a microtitre vessel, incorporating heating means 13 for effecting the reaction and having a lid 20 with a window 22 to enable viewing of the contents of the vessel. The arrangement being such that the heating means also operates to ensure that the window is kept vapour-free. Apparatus within which such reaction vessels are stationed during the reaction has an array of thermopile sensors 52 for measuring vessel temperature.

Nucleic acid quantitation

A method for measuring the amount of nucleic acid present in a sample, said method comprising

Polynucleotide analysis using combinatorial PCR

Sample vessel

Multi-well improved plate

Temperature regulating container

Provided is a temperature regulating container whereby the states of multiple samples can be optically measured in real time while regulating the temperature of the samples and the samples can be moved at high speed by, for example, shaking without requiring a complicated mechanism. A cell culture container provided with a combination of three kinds of members, i.e., a temperature regulating liquid-holding member (110), a sample-holding member (120) and a lid (130). Owing to this combination, the cell culture container enables that: multiple samples (140) are contacted with a temperature regulating liquid (141) so that the temperature of the samples (140) can be increased at high speed and a uniform temperature can be maintained; the lid (130), said lid being disposed so as to be in contact with the surface of the samples (140), prevents the samples (140) from drying and contamination and, at the same time, prevents dew formation on an optical path for performing optical measurement; the ...

Honeycomb tube

Honeycomb tube

Honeycomb tube

DEVICE WITH INTEGRATED MICRO STENCIL

DEVICE AND PROCEDURE FOR THE PROOF OF MOLECULAR RECIPROCAL EFFECTS

CONTAMINATION BARRIER

MICRO PLATE AND MANUFACTURING PROCESS DAF�R

COVER FOR A PCR CONTAINER WITH SAMPLES, WHICH PERMITS PCRAMPLIFIKATION AND THE PROOF OF THE PCR PRODUCT BY HYBRIDIZING WITHOUT OPENING THE PCR CONTAINER

MORE KRYOBEHÄLTER

REACTION CONTAINER WITH CONDUCTIVE LAYER AND NON-METALLIC INTERIOR LAYER

PLATE WITH A MAJORITY OF HOLLOWS FOR THE ADMISSION OF CHEMICAL AND/OR BIOCHEMICAL AND/OR MICRO-BIOLOGICAL SUBSTANCES.

MICRO TEST PLATE.

REACTION CONTAINER ARRANGEMENT FOR A DEVICE FOR THE AUTOMATIC EXECUTION OF TEMPERATURE CYCLES

REACTION CONTAINERS

MICRO TITER PLATE OR CHIP WITH EMBEDDED CARRIER CORE

AUTOMATED POLYMERASE NUCLEAR CHAIN REACTION

THERMOZYKLIERAPPARAT WITH AN AUTOMATICALLY POSITION-CASH COVER

ULTRADÜNNWANDIGE MULTIPLE PERFORATED TILE FOR HEATING BLOCK THERMAL CYCLES

Thermocycler and sample holder

Identification and quantification of a plurality of biological (micro)organisms or their components

INTEGRATED SAMPLE PROCESSING DEVICES

Preparation of glassified biological reagents

The invention related to a method of making a dried reagent preparation, comprising the steps of: providing an aqueous solution of at least one buffered biological reagent; mixing a glass forming filler material with the buffered reagent solution to form a mixture wherein the concentration of the filler material is sufficient to facilitate formation of a glassy, porous composition; dispensing the mixture in the form of substantially uniform droplets into wells of a multi-well container, wherein a single droplet is dispensed into each well; drying the droplets in the container to form the reagent preparation. The reagent preparation is water soluble and has a Tg sufficient for room temperature stability.

Process for Controlling the Temperature of Wastewater Samples

FLUID MANIPULATION IN A MICROFABRICATED REACTION CHAMBER SYSTEM

SLIP COVER FOR HEATED PLATEN ASSEMBLY

Systems and methods for biological analysis

A system for performing biological reactions is provided. The system includes a chip including a substrate and a plurality of reaction sites. The plurality of reaction sites are each configured to include a liquid sample of at most one nanoliter. Further, the system includes a control system configured to initiate biological reactions within the liquid samples. The system further includes a detection system configured to detect biological reactions on the chip. According to various embodiments, the chip includes at least 20000 reaction sites. In other embodiments, the chip includes at least 30000 reaction sites.

Apparatuses and compositions for cryopreservation of cellular monolayers

Provided are apparatuses for cryopreserving cells which include a vessel comprising a biocompatible substrate, wherein the vessel further comprises an interior and an exterior, and a mechanical ice nucleating device disposed in or on the vessel interior for initiating ice crystal formation. Also provided are kits comprising one or more apparatuses for cryopreserving cells and a biopreservation medium. Further provided are compositions comprising a vessel for holding cells, a mechanical ice nucleating device, a biopreservation medium, and cells disposed in the vessel. The apparatuses, kits, and compositions of the invention can optionally include an insulating material which is disposed on all or a portion of the vessel.

Thermal cycler for automatic performance of the polymerase chain reaction with close temperature control

CONDUCTIVE MICROTITER PLATE

The present invention is a multi-well vessel such as a microtiter plate, made from a plastic material formulated for increased thermal conductivity. In a preferred embodiment, the plastic material is a thermally conductive formulation of a cyclic polyolefin, syndiotatic polystyrene, polycarbonate, or liquid crystal polymer, with a melting point greater than 130 ~C and exhibiting very low intrinsic fluorescent properties. A conductive medium, such as conductive carbon black, is included in the formulation of the plastic material at about 5 % or greater by weight to increase thermal conductivity. To further increase thermal conductivity, a thermally conductive ceramic filler, such as a Boron Nitride filler, may be added to the formulation. A polymeric surfactant may also be added to the formulation for increased performance. The invention may also include a flat piece of conductive material attached to the flat bottom of the plate to impart conductivity and flatness to the part. Alternatively ...

TEMPERATURE CONTROLLING METHOD AND TEMPERATURE CONTROLLER

An object of the present invention is to accurately control temperatures of microorganisms or the like. In the present invention, a temperature controller comprises a plurality of cells for storing microorganisms or the like, heaters, and a cooling section. The heaters selectively heat the plurality of cells, and the cooling section wholly cools the plurality of cells. When the maximum value of temperature of a plurality of places is not lower than a first lower limit, the cooling section is driven. When a temperature of one place is not higher than a first lower limit, the heater to heat the place is driven. When the maximum value is not higher than a second lower limit, the cooling section is stopped. When a temperature of one place is not lower than a second upper limit, the heater to heat the place is not driven.

THERMAL CYCLER WITH PROTECTION FROM ATMOSPHERIC MOISTURE

Localized temperature control in a thermal cycler containing a plurality of samples is achieved by an apparatus comprising a multi-receptacle sample block, a thermoelectric module, and a finned heat sink block, all shaped to be capable of arrangement in a stacked configuration in which the sample block is in thermal contact with the thermoelectric module and the thermoelectric module is in thermal contact with the heat sink block. A support frame is sized to receive the sample block, the thermoelectric module, and the finned heat sink block in the stacked configuration. A bar sized to fit between adjacent fins of the finned heat sink block, and a system for affixing the bar to the support frame is used to secure the finned heat sink block against the thermoelectric module when in the stacked configuration.

METHOD AND APPARATUS FOR CONDUCTING AN ASSAY

The present invention relates to methods and apparatus for conducting an assay. In particular, the present invention relates to a rotatable platform which can be used for conducting an assay, in particular multi-step assays. The present invention provides a rotatable platform adapted to immobilise a first binding partner in one or more discrete areas on a surface of said platform, or to selectively immobilise a second binding partner in one or more discrete areas on a surface of said platform. The invention also relates to methods, apparatus, a kit and the use of the rotatable platform for conducting an assay. In particular, the invention has been developed primarily for use in sequencing nucleic acid by pyrosequencing, however the invention is not limited to this field.

MICROFLUIDIC DEVICE AND METHOD OF USING SAME

A microfluidic device comprising a plurality of reaction chambers in fluid communication with a flow channel formed in an elastomeric substrate, a vapor barrier for preventing evaporation from the plurality of reaction chambers, and a continuous phase fluid for isolation of each of the plurality of reaction chambers.

METHOD FOR MEASURING SUBSTANCES BY ENZYME IMMUNOASSAY

OF The DISCLOSURE Improved method for the measurement of substance comprising simultaneously measuring a plurality of a substance to be measured (specimens) by enzyme immunoassay which comprises: a. reacting specimens and a material which will be specifically combined with the substance to be measured and which has been made in the solid phase; b. separating the reaction mixture in the Step (a) into the solid phase and the liquid phase; c. reacting the solid phase with an enzyme-labelled product of a material which will be specifically combined with the substance to be measured; d. separating the reaction mixture in the Step (c) into the solid phase and the liquid phase; e. reacting the solid phase or the liquid phase with a substrate for the enzyme in the Step (c); and f. measuring the optical density of the reaction mixture; in the Step (a), successively contacting the respective specimens with the material which will be specifically combined with the substance to be measured and which ...

DEVICE AND METHOD FOR CARRYING OUT EXPERIMENTS IN PARALLEL

Modular device and method for carrying out experiments in parallel on process substances to develop technical methods, comprising at least a multiplicity of individual reactors which are controllable independently of one another said reactors being comprised of pressure-tight chambers having separable sample vessels, and optionally, stirring devices, heating instruments, cooling instruments, or both, a monitoring unit for monitoring or controlling at least the pressure and the temperature in the individual reactors, pressure-tight lids having independent feed lines and optionally independent discharge lines for individual process substances; a plurality of the lids being simultaneously sealable upon the rectors by a common sealing means (11).

THAWING STATION

A heating device (12) for a titration plate enables selected sample wells (18) to be thawed by providing an array of individually energizable heat sources (26) each capable of heating a single sample well (18). A cold plate (20) serves to maintain all other samples in their frozen state.

SAMPLE HOLDER AND LOCK-IN THERMOGRAPHY SYSTEM

A sample holder (1) for magnetic nanoparticle samples comprises a plurality of sample wells (la) for holding a magnetic nanoparticle sample, which are distributed on a top surface (lb) of the sample holder (1). The sample wells (la) are distributed such that a first distance (dl) between neighboring samples wells (la) and/or a second distance (d2) between each peripheral sample well (la) and a respective edge of the top surface (lb) of the sample holder (1) and/or a third distance (d3) between a deepest point of the sample wells (la) and a bottom of the sample holder (1) is between 1 and 100 times greater than a thermal diffusion length of the sample holder material. The sample holder (1) is used in a lock-in thermography system (2).

SAMPLE PROCESSING DEVICE WITH COMPRESSION SYSTEMS AND METHOD OF USING SAME

Sample processing systems and methods of using those systems for processing sample materials located in sample processing devices are disclosed. The sample processing systems include a rotating base plate on which the sample processing devices are located during operation of the systems. The systems also include a cover and compression structure designed to force a sample processing device towards the base plate. The preferred result is that the sample processing device is forced into contact with a thermal structure on the base plate. The systems and methods of the present invention may include one or more of the following features to enhance thermal coupling between the thermal structure and the sample processing device: a shaped transfer surface, magnetic compression structure, and floating or resiliently mounted thermal structure. The methods may preferably involve deformation of a portion of a sample processing device to conform to a shaped transfer surface.

HONEYCOMB TUBE

A honeycomb tube with a planar frame defining a fluidic path between a first planar surface and a second planar surface. A fluidic interface is located at one end of the planar frame. The fluidic interface has a fluidic inlet and fluidic outlet. The fluidic path further includes a well chamber having an well-substrate with a plurality of wells. The well chamber is arranged in the planar frame between the first or second surface and the well-substrate.

MICROPLATES WITH ULTRA-THIN WALLS BY TWO-STAGE FORMING

A multi-well plate with wells having ultra-thin walls and yet with sufficient structural rigidity to allow reliable use of the plate in automated instrumentation is formed by first forming a plate blank by injection molding, the blank being of a thickness that provides the desired rigidity, and then subjecting the blank to vacuum forming to stretch designated areas in the blank to form wells or to extend wells already formed, the stretching resulting in a reduction in thickness of the molded resin at the walls of the wells only.

SOLID GEL AMPLIFICATION METHOD AND APPARATUS FOR PLATFORM MOLECULAR DIAGNOSTICS

The present invention provides for a novel system and method for amplification and detection of nucleic acids within a miniaturized device wherein sample administration occurs via capillary forces through a channel created by drying a hydrogel containing all components needed for a cell-free, enzymatic, nucleic-acid amplification system other than the template nucleic acid or precursor thereto, and wherein an aqueous sample is provided to the desiccated hydrogel, and the hydrogel is rehydrated, through capillary forces.

COVER FOR ARRAY OF REACTION TUBES

An array of reaction tube covers adapted to seal a plurality of reaction tubes comprises a unitary body of flexible material having a plurality of flexible plastic nodules. Each nodule is adapted to seal one of the reaction tubes. Each of the nodules is flexible held in a predetermined planar spaced relationship from each other in rows, preferably in rows and columns, by an integral web having a plurality of apertures therethrough. Each of the nodules has a downwardly convex, generally hemispherical lower portion extending from the web, an upwardly convex upper portion extending from the web over the lower portion, and a centrally domed nipple extending upwardly from the upper portion.

PIPETTING ROBOT WITH AN IMPROVED TEMPERATURE-CONTROL APPARATUS

A temperature-control apparatus in the form of a plate is described for pipetting robots for holding microtitration plates, which have a spiral tube for a temperature-control medium and which apparatus is divided into at least two segments for holding microtitation plates.

METHOD OF HEATING A SPECIMEN CARRIER

A method and apparatus for heating specimens in wells of a metallic specimen carrier. The specimen carrier is heated by applying resistive heating directly to the carrier. An AC source and transformer may be used where the specimen carrier is in series with a single turn secondary winding of the transformer.

REACTION VESSELS

A reaction vessel for holding reagents is described, which vessel comprises an electrically conducting polymer capable of emitting heat when an electric current is passed through it. Suitably the reaction vessel comprises a reagent container, such as a capillary tube, slide or chip, in close contact with the electrically conducting polymer. For example, the polymer may be in the form of a film which is wrapped around the tube to form a sheath. This provides a readily controllable heating supply which may be heated and cooled to desired temperatures rapidly. An apparatus suitable for thermal cycling reactions, such as the polymerase chain reaction (PCR) and comprising one or more reaction vessels as described above, as well as methods for carrying out such reactions are also described and claimed.

DEVICE FOR REGULATION THE INFLUENCE NEURAMINIDASE ONES.

Method and system for determining biological properties of samples.

Bei einem Verfahren zur Ermittlung biologischer oder anderer Eigenschaften von Proben mittels eines Mikroplatten-Lesegeräts sind die Proben in Näpfchen (112) einer Mikrotiterplatte (110) angeordnet. Das Verfahren umfasst ein Füllen von Näpfchen einer Mikrotiterplatte mit Proben, die mindestens eine Probensubstanz in einem flüssigen Medium enthalten, ein Aufheizen von Proben mittels einer Heizeinrichtung, ein Anordnen der Mikrotiterplatte in einer Messposition innerhalb einer Messkammer des Mikroplatten-Lesegeräts sowie eine Untersuchung von Proben während und/oder nach dem Aufheizen in mindestens einem Betriebsmodus des Mikroplatten-Lesegeräts durch Messung von aus den Proben emittierter Strahlung. Das Verfahren ist dadurch gekennzeichnet, dass zum Aufheizen der Proben Mikrowellenstrahlung verwendet wird. Ein zur Durchführung des Verfahrens geeignetes System (100) hat eine Heizeinrichtung (190) zum Aufheizen von Proben, die sich in Näpfchen (112) einer Mikrotitierplatte (110) befinden.

Verfahren und System zur Ermittlung biologischer Eigenschaften von Proben.

Bei einem Verfahren zur Ermittlung biologischer oder anderer Eigenschaften von Proben mittels eines Mikroplatten-Lesegeräts sind die Proben in Näpfchen (112) einer Mikrotiterplatte (110) angeordnet. Das Verfahren umfasst ein Füllen von Näpfchen einer Mikrotiterplatte mit Proben, die mindestens eine Probensubstanz in einem flüssigen Medium enthalten, ein Aufheizen von Proben mittels einer Heizeinrichtung, ein Anordnen der Mikrotiterplatte in einer Messposition innerhalb einer Messkammer des Mikroplatten-Lesegeräts sowie eine Untersuchung von Proben während und/oder nach dem Aufheizen in mindestens einem Betriebsmodus des Mikroplatten-Lesegeräts durch Messung von aus den Proben emittierter Strahlung. Das Verfahren ist dadurch gekennzeichnet, dass zum Aufheizen der Proben Mikrowellenstrahlung verwendet wird. Ein zur Durchführung des Verfahrens geeignetes System (100) hat eine Heizeinrichtung (190) zum Aufheizen von Proben, die sich in Näpfchen (112) einer Mikrotitierplatte (110) befinden.

METHODS AND SYSTEMS FOR FAST HEATING AT PCR

Multi-parameter integrated type colorimetric pool

The invention discloses a multi-parameter integrated type colorimetric pool, comprising multiple colorimetric pool monomers; the adjacent colorimetric pool monomers are connected by a connecting plateand a heating unit is provided in the monomer colorimetric pool; wherein, the number and form of colorimetric pool monomer in the multi-parameter integrated type colorimetric pool of the invention are composed freely; the monomer colorimetric pool comprises a heating element which is not only used as the colorimetric pool, but also the heater; the temperature is set freely and the use is convenient. The invention is the updated and replaced product of the present colorimetric pool and which is used for the photometric analyzer to improve the stability, reliability of the detection and the measurement precision; therefore the invention is applied to the photometric analyzer widely.

APPARATUS OF THERMAL VALIDATION, TOGETHER Of a DEVICE OF TREATMENT Of BIOLOGICAL SAMPLES AND Of SUCH an APPARATUS, AND MANUFACTORING PROCESS Of SUCH an APPARATUS.

L'appareil de validation thermique (302) comprend au moins une chemise (316), chaque chemise délimitant un puits (314) et étant destinée à être insérée dans une cavité respective du dispositif de traitement thermique, destinée à chauffer ou refroidir des échantillons biologiques, et une sonde de température respective placée dans chaque puits (314). Chaque chemise est en matière plastique.

Comprising Microéprouvette of the bodies increasing surface.

COMPOSITION FOR PREVENTING EVAPORATION OF REACTION SOLUTION DURING NUCLEIC ACID AMPLIFICATION REACTION

THERMALLY-CONDUCTIVE BIOLOGICAL ASSAY TRAYS

A thermally-conductive biological assay tray (10) is provided. The trays are made from a polymer composition comprising a base polymer matrix and a thermally-conductive material. The trays can be used for fluorescent immunoassays. The fluorescence level of the polymer composition is sufficiently low such that it does not interfere with the fluorescent immunoassay process. The invention also includes methods for making the bioassay trays. © KIPO & WIPO 2007 ...

Thermally-conductive biological assay trays

A thermally-conductive biological assay tray (10) is provided. The trays are made from a polymer composition comprising a base polymer matrix and a thermally-conductive material. The trays can be used for fluorescent immunoassays. The fluorescence level of the polymer composition is sufficiently low such that it does not interfere with the fluorescent immunoassay process. The invention also includes methods for making the bioassay trays.

System for conducting the identification of bacteria in biological samples

METHODS AND APPARATUS FOR PERFORMING ARRAY MICROCRYSTALLIZATIONS

MICROSAMPLE CRYOSTORAGE SYSTEMS AND METHODS

A multi-vial assembly for containing a plurality of samples in a cryostorage system includes a plurality of vials each defining a vial chamber to hold a respective sample and coupled together to form an integral vial assembly. The vials are independently removable from the integral vial assembly to permit selective access to the respective samples.

FLUID PROCESSING DEVICE WITH CAPTURED REAGENT BEADS

A fluid processing device, method and system are provided. The fluid processing device can comprise: a substrate; a plurality of reaction regions disposed in or on the substrate; at least one channel interconnecting the plurality of reaction regions, the at least one channel having a cross-sectional area that includes a maximum dimension; and a plurality of reagent-releasing beads. Each reagent-releasing bead can be positioned in a respective one of the reaction regions. Each bead can comprise one or more reaction components for an assay. Each of the reagent- releasing beads can have a minimum dimension that is greater than the maximum dimension of the channel cross-section.

FLUID PROCESSING DEVICE AND METHOD

A fluid processing device and methods are provided that can process one or many different fluid samples, detection for each of which can be multiplexed to detect the presence or absence of each of a panel of target sequences, for example 20 different target sequences. The device can comprise a substrate and one or more fluid processing pathways at least partially defined by the substrate. Each fluid processing pathway can comprise a pre-amplifÊcation region and two or more amplification regions disposed downstream from and in fluid communication with the pre- amplification region. A burstable valve can be disposed along each fluid processing pathway and the downstream regions can contain pre-loaded ammonia gas to draw an amplified sample downstream.

UNIVERSAL RANDOM ACCESS DETECTION OF NUCLEIC ACIDS

Provided herein is technology relating to detecting nucleic acids in a sample and particularly, but not exclusively, to systems and methods related to random access primer pair libraries that are used to configure or customize assays for nucleic acid detection.

APPARATUS AND METHOD FOR CALIBRATION OF NON-CONTACT THERMAL SENSORS

Biochemical assay apparatus uses a container with a sleeve of electrically- conductive material (300) to heat it. The heating is done inside a chamber and a contactless heat sensor (110) such as a thermopile or a bolometer, also inside the chamber, is used to monitor the temperature of the electrically conductive material (300). There are many factors that distort the output of the heat sensor (110), particularly as the temperature rises and properties such as emissivity change, or as time goes by and tarnishing and dust affect the heat sensor output. Because the sleeve has low thermal mass and heat transfer only has to happen over short distances, it is relatively easy to calculate a change in actual temperature of the electrically conductive material (300) when subjected to a known pulse of drive current and this property can be used to calibrate the performance of the heat sensor (110) in situ in the chamber.

REACTION VESSEL AND METHOD OF DNA AMPLIFICATION REACTION

Linear recess is provided on the back of synthetic resin substrate (1). The linear recess is sealed with film (3) so as to form tunnellike reaction chamber (12) surrounded by the film (3) and the substrate. Further, the film (3) is caused to undergo an ingress from the back surface of the substrate (1) into the side of reaction chamber (12) so as to form protrudent part (31). The evaporation of reagents, etc. can be suppressed. Moreover, the occurrence of any gap attributed to a difference of thermal expansion coefficient between the substrate (1) and the film (3) is limited to the side wall of the reaction chamber (12) and the protrudent part (31) of the film (3), so that a satisfactory amount of reaction product can be obtained through prevention of any reduction thereof.

DEVICE FOR ANALYSIS OF A TARGET ANALYTE

This disclosure is directed to a slide for analyzing target analytes of a suspension. The slide can be used to collect and hold the target analyte for imaging or further processing. The slide comprises a well section, including wells into which the target analyte can be stored. The wells may be removable and sized to fit into a second apparatus, such as a PCR thermocycler, for additional processing. Alternatively, the wells may hold processing vessels, such as PCR tubes, the processing vessels being used for downstream processing and/or analysis.

DISPENSER ARRAY SPOTTING

A method of dispensing a fluid comprising (a) aspirating a first fluid volume into a dispenser adapted to dispense fluid volumes of one microliter or less; (b) dispensing a desired volume of the fluid volume to form a dispensed portion; (c) calculating the volume of the dispensed portion; and (d) calculating an adjusted desired volume that compensates for a difference between the desired volume and the volume of the dispensed portion.

SAMPLE WELL PLATE

A sample well plate (10), having an array of sample wells (12) formed therein, has its underside surfaces coated with a highly reflective coating (16), including the outer surfaces of the sample wells (12). The coating is preferably formed from metal, for example silver or nickel. The sample well plate (10) is formed of a light transmissive material to ensure the full benefit of the reflectivity of the coating (16). The coating provides substantial increases in reflectivity compared with prior art sample well plates.

REACTION VESSEL

A reaction vessel for use in a clinical analyzer includes a frame having a plurality of vertically disposed reaction chambers in spaced relation, each of the reaction chambers being sized for retaining a volume of at least one fluid. The vessel further includes at least one gap region defined between at least one adjacent pair of reaction chambers for thermally affecting the fluid contents therein.

FLUID MANIPULATION IN A MICROFABRICATED REACTION CHAMBER SYSTEM

The present invention relates to a microfabricated reaction chamber system and a method of fluid transport. The system may be used, for example, in a method of carrying out a nucleic acid sequence amplification and detection process on a nucleic acid sample. The microfabricated chamber system comprises an inlet port and/or an outlet port and a variable volume chamber in fluid communication with said port(s), wherein altering the volume of the variable volume chamber effects and/or restricts flow of a fluid sample to and/or from said port(s).

LOW-MASS SAMPLE BLOCK WITH RAPID RESPONSE TO TEMPERATURE CHANGE

A sample block for use in the polymerase chain reaction, DNA sequencing, and other procedures that involve the performance of simultaneous reactions in multiple samples with temperature control by heating or cooling elements contacting the bottom surface of the block is improved by the inclusion of hollows in the block that are positioned to decrease the mass of the block in the immediate vicinity of the wells.

AUTOMATIC DEVICE FOR THE PHOTOMETRIC ANALYSIS OF LIQUID SAMPLES

A device (2) for the photometric analysis of liquid samples has the advantage of allowing a plurality of samples which require different processing steps (different incubation and measurement times) to be processed. It is therefore possible to carry out in a relatively short period more than twenty different types of tests with this device. A device for gripping objects (41) and a process for analysing liquid samples inside vessels by means of a device for the automatic analysis of liquid samples are disclosed. A process is disclosed for measuring samples, according to which the sample material is transferred from a sample container into a vessel, is processed and is subjected to at least one measurmenet process, then the corresponding measurement value is stored.

INTEGRATED MICROARRAY DEVICES

This invention relates generally to the field of microarray technology. In particular, the invention provides an integrated macroarray device, which device comprises a substrate comprising a plurality of distinct microlocations and a plurality of microarray chips, wherein the number of said microlocations equals to or is more then the number of said microarray chip. In preferred embodiments, the devices also comprises a temperature controller at some or all of the microlocations. The use of the integrated microarray devices for detecting interactions among various moieties in various fields, such as clinical diagnostics, drug discovery, environmental monitoring and forensic analysis, etc., are further provided.

APPARATUS AND METHODS FOR MULTIPLEX ANALYSES

The present invention provides miniaturized instruments for conducting chemical reactions where control of the reaction temperature is desired or required. Specifically, this invention provides chips and optical systems for performing and monitoring temperature-dependent chemical reactions. The apparatus and methods embodied in the present invention are particularly useful for high-throughput and low-cost amplification of nucleic acids.

PCR SAMPLE HANDLING DEVICE

A device (30) for handling PCR microcards (10), each having an array of sample chambers (14) closed by a transparent material on one side thereof, in relation to a PCR instrument, the device (30) including a carrier (30) having an apertured region with an array of holes (48) corresponding in number and relative location with the array of sample chambers (14) in each of the microcards (10), and a provision for retaining a microcard (10) on the carrier (30) so that the transparent material faces the apertured region, and so that the side of the microcard (10) opposite the transparent material is unobstructed at least throughout the array of sample chambers (14). The device (10) cooperates with the PCR instrument to ensure accurate positioning of the carrier and the microcard retained thereon for real time PCR processing.

FLEXIBLE HEATING COVER ASSEMBLY FOR THERMAL CYCLING OF SAMPLES OF BIOLOGICAL MATERIAL

A flexible heating cover assembly (200) for an apparatus for heating samples of biological material with substantial temperature uniformity includes a housing having a plurality of engageable enclosure components; a resistive heater (300) having a plurality of heater element areas; a heater backing plate (350) providing stability to the resistive heater (300); a force distribution system that distributes a force over the heater backing plate (350); and a support plate (500) providing stiffness for the force distribution system, wherein the arrangement of the resistive heater (300), the heater backing plate (350), the force distribution system and the support plate (500) provide substantial temperature uniformity among a plurality of sample tubes (140) for receiving samples of biological material. The flexible heating cover assembly (200) improves the uniformity, efficiency, quality, reliability and controllability of the thermal response during thermal cycling of the biological material ...

PCR PLATE COVER AND MAINTAINING DEVICE

A cover (10) for a PCR plate having multiple wells including a rigid sheet (11), and a resiliently compliable sheet (12) cooperating with one side of the substantially rigid sheet (11) to press a sealing sheet (35) against the surface of the PCR plate (30) to maintain a seal in the wells. The cover (10) further includes at least one retaining device (13) attached to the rigid sheet (11) to engage with the PCR plate (30) and retain the substantially rigid sheet (11) and the resiliently compliable sheet (12) in a condition to press the sealing sheet (35) against the surface of the PCR plate (30). Also disclosed is an assembly that includes a PCR plate (30) and a cover (10) that is designed to prevent sample loss.

Heat conducting sample block

Disclosed herein is a heat-conducting sample block that includes a top plate and a base plate, each having upper and lower faces, the upper face of the top plate having a recess therein. The recess has an opening for accepting a sample or sample vessel, and the lower face of the top plate has a projection extending towards and fixedly engaged with a notch on the upper face of the base plate.

Microfluidic device for carrying out a reaction

A device for carrying out a reaction, which device comprises a wafer provided with a group of at least two wells. The wells are thermally separate from each other by means of a groove in a layer of the device, while parts separated by the groove are locally connected by bridges. In this way a device is provided combining great mechanical strength with good thermal insulation.

Apparatus and method for calibration of non-contact thermal sensors

Biochemical assay apparatus uses a container with a sleeve of electrically-conductive material ( 300 ) to heat it. The heating is done inside a chamber and a contactless heat sensor ( 110 ) such as a thermopile or a bolometer, also inside the chamber, is used to monitor the temperature of the electrically conductive material ( 300 ). There are many factors that distort the output of the heat sensor ( 110 ), particularly as the temperature rises and properties such as emissivity change, or as time goes by and tarnishing and dust affect the heat sensor output. Because the sleeve has low thermal mass and heat transfer only has to happen over short distances, it is relatively easy to calculate a change in actual temperature of the electrically conductive material ( 300 ) when subjected to a known pulse of drive current and this property can be used to calibrate the performance of the heat sensor ( 110 ) in situ in the chamber.

Thermal validation apparatus, assembly including a device for the thermal processing of biological samples and such an apparatus, and method for manufacturing such an apparatus

The invention relates to a thermal validation apparatus including at least one lining, each lining defining a sink and intended to be inserted into a respective recess of the thermal processing device for heating or cooling biological samples, as well as a respective temperature probe arranged in each sink. Each sleeve is made of a plastic material.

Biological microfluidics chip and related methods

A biological microfluidics chip ( 100 ) comprising a substrate ( 102 ), a microfluidic inlet port ( 104 ) defining an opening in a surface of the substrate ( 102 ), and a microfluidic outlet port ( 106 ) defining an opening in a surface of the substrate ( 102 ). The biological microfluidics chip ( 100 ) also comprises a plurality of wells ( 108 ) extending from a top surface ( 110 ) of the substrate, wherein each well ( 108 ) is bounded by one or more walls, and an inlet opening ( 112 ) and an outlet opening ( 114 ) are provided in a wall of each of the plurality of wells ( 108 ). An inlet microfluidic channel ( 116 ) is provided in the substrate ( 102 ) to connect the microfluidic inlet port ( 104 ) to each of the inlet openings ( 112 ) in the walls of the wells, and an outlet microfluidic channel ( 118 ) is provided in the substrate to connect each of the outlet openings ( 114 ) in the walls of the wells to the microfluidic outlet port ( 106 ).

Instrument And Method For The Automated Thermal Treatment Of Liquid Samples

An instrument and a method for the automated thermal treatment of liquid samples are disclosed. An inter-distance between a temperature-controlled receptacle for loading with a plurality of vessels for containing the samples and end portions of optical fibers can be varied, wherein the receptacle is configured to form a thermal communication with the loaded vessels and wherein the optical fibers have first and second end portions. The first end portion and the second end portion of each optical fiber is fixed with respect to each other for transmitting light, wherein the variation of the inter-distance allows the vessels to be loaded to or unloaded from the receptacle and to enable detection of light from the samples contained in the one or more receptacle-loaded vessels.

Multilayer high density microwells

A multilayer well device includes a first substrate comprising an array of wells having a first pattern disposed therein and a second substrate comprising an array of wells having a second pattern, complementary to the first pattern disposed therein, wherein the second substrate is secured adjacent to a face of the first substrate. A common channel is interposed between the array of wells of the respective first and second substrates and is coupled to an inlet and an outlet.

Microfluidic flow cell

A microfluidic flow cell having a body with a fluid transport channel disposed therein, the fluid transport channel having a proximal end and a distal end defining a fluid flow path, a fluid inlet port disposed at the proximal end of the fluid transport channel at a central portion of the body and an outlet port disposed at the distal end of the fluid transport channel at an outer portion of the body, and a plurality sample wells disposed in the fluid transport channel substantially perpendicular to the fluid flow path in the fluid transport channel. The microfluidic flow cell may have hundreds or thousands of individual, sub-microliter sample wells. The microfluidic flow cell can be filled by applying a flowable liquid to the inlet port and spinning the flow cell to cause fluid to flow into fluid transport channel.

System and method including analytical units

Systems and methods for processing and analyzing samples are disclosed. The system may process samples, such as biological fluids, using assay cartridges which can be processed at different processing locations. In some cases, the system can be used for PCR processing. The different processing locations may include a preparation location where samples can be prepared and an analysis location where samples can be analyzed. To assist with the preparation of samples, the system may also include a number of processing stations which may include processing lanes. During the analysis of samples, in some cases, thermal cycler modules and an appropriate optical detection system can be used to detect the presence or absence of certain nucleic acid sequences in the samples. The system can be used to accurately and rapidly process samples.

System and method including analytical units

Systems and methods for processing and analyzing samples are disclosed. The system may process samples, such as biological fluids, using assay cartridges which can be processed at different processing locations. In some cases, the system can be used for PCR processing. The different processing locations may include a preparation location where samples can be prepared and an analysis location where samples can be analyzed. To assist with the preparation of samples, the system may also include a number of processing stations which may include processing lanes. During the analysis of samples, in some cases, thermal cycler modules and an appropriate optical detection system can be used to detect the presence or absence of certain nucleic acid sequences in the samples. The system can be used to accurately and rapidly process samples.

Apparatus and Methods for Integrated Sample PReparation, Reaction and Detection

Cartridges for the isolation of a biological sample and downstream biological assays on the sample are provided, as are methods for using such cartridges. In one embodiment, a nucleic acid sample is isolated from a biological sample and the nucleic acid sample is amplified, for example by the polymerase chain reaction. The cartridges provided herein can also be used for the isolation of non-nucleic acid samples, for example proteins, and to perform downstream reactions on the proteins, for example, binding assays. Instruments for carrying out the downstream biological assays and for detecting the results of the assays are also provided.

Reagent slot, sample tray and combination kit used in biochemical test

The present invention relates to a reagent slot, a sample tray and a combination kit used in biochemical test; this reagent slot is used to be mounted in the sample tray. Specifically, the reagent slot has a main frame which has plural holding holes, and the sample tray includes a plate portion, which is equipped with plural inserting holes and plural inserting slots, and a support portion which is used to support the plate portion.

METHODS AND COMPOSITIONS FOR PAPER-BASED AND HYBRID MICROFLUIDIC DEVICES INTEGRATED WITH NUCLEIC ACID AMPLIFICATION FOR DISEASE DIAGNOSIS

Certain embodiments are directed to paper and its hybrid microfluidic devices integrated with nucleic acid amplification for simple, cost-effective, rapid, and sensitive pathogen detection, especially in low-resource settings. 1. A device comprising:an amplification layer having at least one amplification well comprising an amplification mixture; anda detection layer comprising at least one detection well comprising a detection reagent absorbed to paper.2. The device of claim 1 , further comprising a top layer comprising the inlet reservoir and microchannels connected to the amplification layer.3. The device of claim 1 , further comprising a support layer.4. The device of claim 1 , wherein the amplification layer and detection layer are the same.5. The device of claim 1 , wherein the amplification well and detection well are the same well.6. The device of wherein the device comprises a plurality of amplification wells.7. The device of claim 2 , wherein the top layer is a polymer.8. The device of claim 7 , wherein the polymer is polydimethysiloxane (PDMS).9. The device of claim 1 , wherein the amplification layer is a polymer.10. The device of claim 9 , wherein the amplification layer comprises a paper insert preloaded with DNA primers.11. The device of claim 9 , wherein the polymer is polydimethysiloxane (PDMS).12. The device of claim 3 , wherein the support layer is glass.13. The device of claim 1 , wherein all layers are paper layers.14. The device of claim 13 , wherein the amplification layer further comprises multiple sublayers.15. The device of claim 14 , wherein the sublayers are cut to form amplification wells.16. The device of claim 13 , wherein the device contains a detection layer comprising detection wells linked to reagent delivery channels.17. The device of claim 1 , further comprising a sequence specific probe in the detection well.18. The device of claim 2 , wherein the top layer further comprises a filter configured to remove components of the sample ...

Integrated system for nucleic acid amplification and detection

Devices, systems, methods, and kits are disclosed for amplifying small quantities of nucleic acid and identifying the nucleic acid sequences of the amplified nucleic acid products.

Method for detecting the presence of a target nucleic acid sequence in a sample

A method comprises loading a sample portion into a sample chamber which comprises means for minimizing diffusion of the sample portion, subjecting the sample portion to an amplification step, and determining whether the sample portion contains at least one molecule of a target nucleic acid. If the sample portion contains a single molecule of the target nucleic acid, the sample portion would attain a detectable concentration of the target nucleic acid after a single round of amplification. Also, a microfluidic device comprising a sample portion and a sample chamber comprising means for minimizing diffusion of the sample portion. Also, a microfluidic device comprising a sample chamber and an amplification targeting reagent positioned in the first sample chamber.

GENETIC ARRAY FOR SIMULTANEOUS DETECTION OF MULTIPLE SALMONELLA SEROVARS

Disclosed are novel genetic arrays for use in the molecular detection of multiple serovars, common food-borne and water-borne pathogens. The arrays may be used to simultaneously detect multiple food safety serovars. The multiplex-detection methods have improved sensitivity and specificity for the detection of multiple high-impact food-borne pathogens simultaneously. Real-time PCR assaying techniques using such serovars include microarrays. 1. An array for testing a sample simultaneously for{'i': 'Salmonella', 'a plurality of serovars that are foodborne pathogens or waterborne agents, said array comprising{'i': 'Salmonella', 'two or more primer pairs placed in a plurality of locations on a microplate for receiving test samples, said two or more primer pairs being adapted to detect and distinguish with specificity DNA from at least two different serovars that are foodborne pathogens or food threat agents,'}wherein all said primer pairs have similar melting temperatures such that they can be simultaneously run under the same PCR conditions, and said primer pairs comprising at least one primer selected from the group consisting of primers shown in Tables 3a and 3b.2Salmonella. A multiplex real-time PCR system for use in simultaneously testing for a plurality of serovars that are foodborne pathogens or food threat agents , said system comprising:{'i': 'Salmonella', 'a container for receiving test samples, said container containing two or more primer pairs, said two or more primer pairs being adapted to detect and distinguish with specificity DNA from at least two different serovars that are foodborne pathogens or food threat agents,'}wherein all said primer pairs have similar melting temperatures such that they can be simultaneously run under the same PCR conditions, and wherein said primer pairs comprise at least one primer selected from the group consisting of primers shown in Tables 3a and 3b.3Salmonella. The system according to claim 2 , the container further ...

METHOD OF MANUFACTURING MICRO CHAMBER PLATE WITH BUILT-IN SAMPLE AND ANALYTIC MICRO CHAMBER PLATE, ANALYTIC MICRO CHAMBER PLATE AND APPARATUS SET FOR MANUFACTURING ANALYTIC MICRO CHAMBER PLATE WITH BUILT-IN SAMPLE

A method of manufacturing a micro-chamber plate with a built-in sample, including: settling a micro-chamber plate for sample injection at a micro-chamber plate receiving part formed with an upper opening; disposing a cover for micro-chamber plate receiving part to cover the upper opening, the cover for micro-chamber plate receiving part having a provisional storing part and an auxiliary covering part connected with the provisional storing part and formed with a through-hole for auxiliary covering part; and manufacturing a micro-chamber plate with a built-in sample by putting the micro-chamber plate receiving part, on which the cover is disposed, into a centrifugal separator which can apply vacuum, applying centrifugal force and injecting a sample solution provisionally stored in the provisional storing part into the micro-chamber plate through a vessel communication part which is formed at the provisional storing part to be communicated with the micro-chamber plate receiving part. 1. A method of manufacturing a micro-chamber plate with a built-in sample , comprising:{'b': 20', '100', '200, 'a step S of settling a micro-chamber plate for sample injection at a micro-chamber plate receiving part formed with an upper opening;'}{'b': 30', '310', '200', '310', '312', '314', '312', '314', '1, 'a step S of disposing a cover for micro-chamber plate receiving part so as to cover the upper opening of the micro-chamber plate receiving part , the cover for micro-chamber plate receiving part comprising a provisional storing part and an auxiliary covering part connected with the provisional storing part and formed with a through-hole - for auxiliary covering part; and'}{'b': 40', '100', '200', '310', '312', '100', '312', '200, 'a step S of manufacturing a micro-chamber plate A with a built-in sample by putting the micro-chamber plate receiving part , on which the cover for micro-chamber plate receiving part is disposed, into a centrifugal separator which can apply vacuum, applying ...

LED DRIVEN PLASMONIC HEATING APPARATUS FOR NUCLEIC ACIDS AMPLIFICATION

Systems and methods for plasmonic heating by combined use of thin plasmonic film-based 2D and 3D structures and a light-emitting diode (LED) for nucleic acids amplification through fast thermal cycling of polymerase chain reaction (PCR) are described. 1. A method for nucleic acids amplification , comprising:disposing a sample within one or more wells comprising a thin film;directing a light source at the thin film to generate light-to-heat conversion within the thin film; andheating the sample as a result of light-to-heat conversion within the thin film.2. The method of claim 1 , further comprising: focusing light from the light source at the one or more wells.3. The method of claim 1 , further comprising: monitoring a temperature of the sample.4. The method of claim 1 , further comprising: controlling one or more of data acquisition from a temperature sensor and actuation of the light source.5. The method of claim 1 , wherein controlling actuation of the light source comprises controlling one or more of exposure duration and injection current at the thin film.6. The method of claim 1 , wherein the thin film comprises an Au film with a nanometer sized grain to enhance light absorption through surface plasmon resonance.7. The method of claim 1 , wherein the one or more wells are formed in a translucent or transparent platform; and wherein the light from the light source is directed through at least a portion of the platform to the thin film.8. The method of claim 1 , further comprising: diffusing focused light to evenly distribute the light to the thin film.9. The method of claim 1 , wherein the light is emitted at a wavelength selected for maximum light absorption within the thin film.10. The method of claim 1 , further comprising: detecting a fluorescence signal within the sample. This application is a division of U.S. patent application Ser. No. 15/649,328 filed on Jul. 13, 2017, incorporated herein by reference in its entirety, which is a 35 U.S.C. § 111(a) ...

Tube strip handling and heating apparatus

A tube strip handling and heating frame having a rectangular outer frame having two spaced longitudinal walls extending in a longitudinal direction, and two spaced lateral walls extending in a lateral direction and extending between respective ends of the longitudinal walls. The outer frame forms an enclosure having an open upper side and an open lower side. The frame also has a number of tube strip wells located inside the outer frame, each tube strip well has a slot elongated in the lateral direction, and each slot is configured to receive a respective tube strip through the open upper side and support the respective tube strip in a vertical direction. The tube strip wells are arranged in a series along the longitudinal direction.

Methods and systems and related compositions for mixtures separation with a solid matrix

Methods and systems and related compositions for separating through a solid matrix a mixture comprising a nucleic acid together with a target compound having a water solubility equal to or greater than 0.01 mg per 100 mL, which can be used for managing fluid flow, biochemical reactions and purification of the nucleic acid or other target analytes.

DIGITAL PCR DEVICE

A digital PCR device comprising: a discrete heating area made of a heat conductive material disposed on a surface that is electrically non-conductive, the discrete heating area comprising a plurality of integral wells configured to contain and partition a DNA sample therein; and at least one conductive trace configured to be connected to a dc voltage source and to heat the plurality of integral wells to a uniform temperature when connected to the dc voltage source, the at least one conductive trace disposed on the surface in an undulating configuration at least partially around the discrete heating area. 1. A digital PCR device comprising:a discrete heating area made of a heat conductive material disposed on a surface that is electrically non-conductive, the discrete heating area comprising a plurality of integral wells configured to contain and partition a DNA sample therein; andat least one conductive trace configured to be connected to a dc voltage source and to heat the plurality of integral wells to a uniform temperature when connected to the dc voltage source, the at least one conductive trace disposed on the surface in an undulating configuration at least partially around the discrete heating area, wherein each of the plurality of integral wells is defined by well walls comprising a thickness of the discrete heating area.2. The digital PCR device of claim 1 , wherein the plurality of integral wells are provided as a regular array in the discrete heating area and wherein wells in a same row of the array are in fluid communication with each other via openings in the well walls.3. The digital PCR device of claim 2 , wherein the openings are collinear along each row of wells.4. The digital PCR device of claim 1 , further comprising a top layer provided over the surface claim 1 , over the discrete heating area comprising the plurality of integral wells claim 1 , and over the at least one conductive trace.5. The digital PCR device of claim 1 , wherein the surface ...

Digital Assay

Provided herein is a method and device for partitioning a fluidic sample. The device contains a plate containing microwells. The method comprises depositing a sample on one or both of the plates when the plates are in an open configuration, wherein the deposition is in the form of a single or multiple droplet of the sample, wherein at least one of the droplets has a volume that occupies more than two microwells and closing the plates to the closed configuration to partition the sample in the microwells. 1. A device for performing a digital assay comprising: (a) the first and second plates are movable relative to each other into different configurations including an open configuration and a closed configuration, and have, on their respective surface, a sample contact area for contacting a fluidic sample that contains an analyte;', 'and', '(b) the second plate has, in the sample contact area, the microwells, wherein each microwell has (i) a predetermined and known geometry, (ii) a well depth of 200 um or less, and (iii) a volume substantially less than that of the fluidic sample;'}, '(c) the spacers are on one or both of the plates, wherein the spacer has a pillar shape and uniform height;, 'a first plate, a second plate, spacers, and microwells, whereinwherein in an open configuration, the average spacing between the inner surface of the first plate and a rim of the microwells in the second plate is larger than the depth of the well and the sample is deposited on one or both of the plates; andwherein in a closed configuration, which is the configuration after the sample is deposited in the open configuration; in the closed configuration, at least a part of the sample is inside the microwells, and the average spacing between the inner surface of the first plate and the rim of the microwells in the second plate is regulated by the spacers to a spacing of ½ (one half) of the microwell depth or less.2. An apparatus comprising a thermal cycler and the device of .3. An ...

Devices, processes, and systems for determination of nucleic acid sequence, expression, copy number, or methylation changes using combined nuclease, ligase, polymerase, and sequencing reactions

The present invention relates to methods, devices, instruments, processes, and systems for the highly specific, targeted molecular analysis of regions of human genomes and transcriptomes from the blood, i.e. from cell free circulating DNA, exosomes, microRNA, IncRNA, circulating tumor cells, or total blood cells. The technology enables highly sensitive identification and enumeration of mutation, expression, copy number, translocation, alternative splicing, and methylation changes using spatial multiplexing and combined nuclease, ligation, polymerase, and sequencing reactions. Such technology may be used for non-invasive early detection of cancer, non-invasive cancer prognosis, and monitoring both treatment efficacy and disease recurrence of cancer.

SAMPLE HOLDER, METHOD FOR MANUFACTURING THE SAMPLE HOLDER, AND APPARATUS FOR RECEIVING THE METALLIC SAMPLE HOLDER

The present invention relates in a first aspect to a metallic sample holder (), in particular for capturing sample volumes for digital polymerase chain detection. The sample holder () comprises an array of indentations (), wherein each indentation () is adapted to capture a maximal sample volume v, with v=2 nl, in particular with v=1 nl, in particular with v=0.8 nl. Each indentation () of the array () has an area cross-section section a, with a ≤8*10mm, in particular with a ≤5*10mm. A second aspect of the invention relates to a method for manufacturing the sample holder (). A third aspect of the invention relates to an apparatus (), in particular for polymerase chain reaction detection, adapted for receiving the metallic sample holder (). A fourth aspect of the invention relates to the use of the sample holder () by means of the apparatus (). 11. A metallic sample holder () , in particular for capturing sample volumes for digital polymerase chain detection reactions , comprising{'b': '10', 'an array of indentations (),'}{'b': '11', 'sub': max', 'max', 'max', 'max, 'wherein each indentation () is adapted to capture a maximal sample volume v, with v=2 nl, in particular with v=1 nl, in particular with v=0.8 nl,'}{'b': 11', '10, 'sup': −3', '2', '−3', '2, 'wherein each indentation () of the array () has an area cross-section a, with a≤8*10mm, in particular with a 5*10mm,'}{'b': '11', 'wherein the indentations () have a bottom area.'}21101111. The metallic sample holder () of claim 1 , comprising an array () with at least 10 claim 1 ,000 indentations () claim 1 , in particular at least 40 claim 1 ,0000 indentations ().31. The metallic sample holder () according to claim 1 , consisting of aluminum claim 1 , silver claim 1 , gold claim 1 , copper claim 1 , or alloys thereof.4111111. The metallic sample holder () according claim 1 , wherein each indentation () is of cylindrical shape claim 1 , of conical shape claim 1 , or of elliptical cone shape and/or has at least ...

SPECIMEN TREATMENT DEVICE, MEASUREMENT DEVICE, AND MEASUREMENT METHOD

Disclosed is a specimen treatment device for treating a specimen accommodated in a reaction container, the specimen treatment device comprising: an installation unit in which the reaction container is installed; a temperature adjusting unit for warming the reaction container installed in the installation unit; and an opening/closing member which can move between a first position at which the temperature adjusting unit is uncovered and a second position at which the temperature adjusting unit is covered. 1. A specimen treatment device for treating a specimen accommodated in a reaction container , the specimen treatment device comprising:an installation unit in which the reaction container is installed;a temperature adjusting unit for warming the reaction container installed in the installation unit; andan opening/closing member which is movable between a first position at which the temperature adjusting unit is uncovered and a second position at which the temperature adjusting unit is covered.2. The specimen treatment device according to claim 1 , whereinthe first position is a position at which access of the reaction container to the temperature adjusting unit through the installation unit is permitted, andthe second position is a position at which the temperature adjusting unit is covered and the access of the reaction container to the temperature adjusting unit is blocked.3. The specimen treatment device according to claim 1 , wherein the opening/closing member is configured to move from the second position to the first position in conjunction with operation to install the reaction container in the installation unit.4. The specimen treatment device according to claim 3 , wherein the opening/closing member includes a first pressed part which is pressed against the reaction container claim 3 , and is configured to move toward the first position by pressing the first pressed part by the reaction container that is moved toward the installation unit.5. The specimen ...

NUCLEIC ACID ANALYZER AND NUCLEIC ACID ANALYZING METHOD

A nucleic acid analyzer includes a first container setting part that sets a first container including reagent storages which store reagents for nucleic acid extraction such that the reagent storages are provided along an X-axis extending in a longitudinal direction of the first container; a dispensing unit that transfers, along the-X axis from the first container, extraction liquid containing nucleic acids extracted in the first container using the reagents for nucleic acid extraction; a second container setting part that is provided along the X-axis and sets a second container, the second container including an injection port through which the extraction liquid transferred by the dispensing unit is injected, storages that store reagents for amplifying the nucleic acids in the extraction liquid, and a flow path that connects the injection port and the storages; and a detector that detects a nucleic acid amplification reaction, which occurs in the storages. 1. A nucleic acid analyzer comprising:a first container setting part that sets a first container including reagent storages which store reagents for nucleic acid extraction such that the reagent storages of the first container are provided along a first axis extending in a longitudinal direction of the first container;a dispensing unit that transfers, along the first axis from the first container, extraction liquid containing nucleic acids extracted in the first container using the reagents for nucleic acid extraction;a second container setting part that is provided along the first axis and sets a second container, the second container including an injection port through which the extraction liquid transferred by the dispensing unit is injected, storages that store reagents for amplifying the nucleic acids in the extraction liquid, and a flow path that connects the injection port and the storages of the second container; anda detector that detects a nucleic acid amplification reaction which occurs in the storages ...

REACTION VESSEL HOLDER AND MOLECULE DETECTION DEVICE

A reaction vessel holder () receives a reaction vessel (). The reaction vessel () has a portion that is substantially optically transparent to light of a first range of wavelengths. The reaction vessel holder () comprises a body having a high thermal conductivity that is thermally coupled to and supports the reaction vessel (). The body is further thermally coupled to a thermal device () for heating or cooling the reaction vessel holder () and thereby the reaction vessel (). The body comprises a transparent portion that is substantially optically transparent to the light of the first range of wavelengths. The optically transparent portion of the reaction vessel () faces the transparent portion of the body such that light of the first range of wavelengths to and/or from the sample in the reaction vessel () can pass through the transparent portion. 1. A reaction vessel holder for receiving a reaction vessel , the reaction vessel for containing a sample and having at least one portion that is substantially optically transparent to light of at least a first range of wavelengths , the reaction vessel holder comprising:a body having a high thermal conductivity, the body being arranged to thermally couple to and support the reaction vessel, the body being further arranged to thermally couple to a thermal device that heats or cools the reaction vessel holder and thereby the reaction vessel, the body comprising at least one transparent portion that is substantially optically transparent to the light of at least the first range of wavelengths, such that the optically transparent portion of the reaction vessel is adapted to face the transparent portion of the body such that light of the first range of wavelengths to and/or from the sample in the reaction vessel pass through the transparent portion.2. (canceled)3. The reaction vessel holder of claim 1 , wherein the thermal conductivity of the body is between about 1800 WmKand about 2100 WmK.4. The reaction vessel holder of ...

Portable, insulated capsules for cryopreservation of biological materials

This invention consists in portable cryostat capsules built in several materials, dimensions and configurations according to the size of the biological refrigeratable samples to be cryopreserved and its cold storage requirements of these biological materials including umbilical cord blood, cells, semen, oocytes, tissues and bigger biological units such as organs for transplantation, entire animals or plants, including human organs or entire bodies in the cryo-medical practice. These capsules are all built in a form of thermoplastic cryostat with a cold source that is in contact with a myogenic tank, a cryo-liquid generator, a cryo-coolant unit and/or an hybrid unit (combination of an external cryogen supply in continuous flow with a closed cycle unit) and includes an outer casing and an inner vessel covered by a thermal insulating material consisting of vacuum, perlite or similar thermal insulator to inhibit the transfer of heat for the correct vitrification of the biological materials. 1. A Portable , insulated capsule for cryopreservation of biological materials: A device (herein named Ion-Dewar) , which can be used in biological , veterinary or medical practice for both the process of controlled cooling in field conditions and the long term cryopreservation of cells , tissues , organs , portions of organs or entire bodies , and also involves equipment and a method for long term storing of these biological materials under a cryogenic temperature , comprising a thermally insulated container made of steel , aluminum , duralumin , copper , thereto-stable of plastic materials or different alloys with at least one thermal insulation layer made of perlite or vacuum , an outer radiation shielding protection and a specific cold source like LN2 connected to the container that keeps such biological materials at a cryogenic temperature.2. The cryopreservation capsule device according to claim 1 , wherein the cryostat can be set in an hermetically closed mode for shipping the ...

MICROTITER PLATE TEMPERATURE CONTROL

The present invention provides methods and apparatus for efficiently providing accurate temperature control of a microtiter cold plate and precise alignment of the microtiter cold plate with a microtiter plate. 1) A microtiter cold plate comprising a single rigid body with a flat top surface with several mechanical stops protruding from said top surface , coolant channels in the opposite surface , coolant fitting ports on one or more sides , a cover plate sealing said coolant channels , insulation over all but the top side , and support legs extending from the rigid body underside protruding through said insulation.2) The microtiter cold plate of wherein the microtiter plate comprises between about 3 to 8 support legs.3) The microtiter cold plate of claim 2 , wherein the plate comprises 4 support legs.4) The microtiter cold plate of claim 2 , wherein the plate comprises 6 support legs.5) The microtiter cold plate of where the cover plate is welded to the rigid body.6) The microtiter cold plate of where the cover plate is brazed to the rigid body.7) The microtiter cold plate of where a sealant or gasket seals the cover plate to the rigid body.8) The microtiter cold plate of where an o-ring seal the cover plate to the rigid body.9) The microtiter cold plate of where the support legs protrude through the insulation by 0.5-3 mm claim 1 , more commonly 0.5-1.5 mm claim 1 , most commonly 1 mm.10) The microtiter cold plate of where the insulation comprises two parts claim 1 , a flexible foamed plastic piece over the bottom and four sides of the rigid body and a hard plastic cover.11) The microtiter cold plate of where the coolant ports are located on one side.12) The microtiter cold plate of where the coolant ports are located at the top of the interior coolant channels.13) The microtiter cold plate of where the coolant ports are located on two separate sides.14) The microtiter cold plate of where the coolant channels form a serpentine pattern.15) The microtiter cold plate ...

RAPID HEATING RATE ARTICLE FOR MICROWAVE METHODS

A microwave susceptor article having an advantageous microwave heating rate property and cooling rate property, including: a substrate; a metallic-containing layer on at least one major surface of the substrate; and optionally a protective coating on or associated with the metallic layer, as defined herein. Also disclosed are a method of making the article and a method of using the article. 1. A microwave susceptor article comprising: a substrate having a thickness of from 2 to 10 mm; and a metallic-containing layer having a thickness of 10 to 800 nanometers on at least one major surface of the substrate , wherein the article has a microwave heating rate of from 8 to 45° C./sec.2. The article of wherein the substrate has: a thermal conductivity of from 0.002 to 0.004 Cal/cm−sec-° C.; a modulus of rupture (MOR) of from 40 to 550 MPa; and a coefficient of thermal expansion (CTE) of from 0 to 92×10/° C.3. The article of wherein the metallic-containing layer comprises a metal sheet claim 1 , metal particles claim 1 , metal wires claim 1 , metal coils claim 1 , metal wool claim 1 , metal fibers claim 1 , metal coated fibers claim 1 , metal coated particles claim 1 , or a combination thereof.4. The article of wherein the article has an ambient cooling rate claim 1 , from an elevated temperature to ambient temperature claim 1 , of from 10° C. per second to 75° C. per second.5. The article of wherein the microwave energy to achieve the microwave heating rate was from 50 to 2000 W claim 1 , and the microwave source frequency is selected from 915 MHz claim 1 , 2.45 GHz claim 1 , 5.8 GHz claim 1 , or combinations thereof.6. The article of wherein the microwave energy to achieve the microwave heating rate was from 1000 to 1400 W claim 1 , and the microwave source frequency was 2.45 GHz.7. The article of wherein the substrate has thickness of from 2 to 6 mm claim 1 , and the metallic-containing layer has thickness of from 10 to 500 nanometers.8. The article of wherein the ...

Field pathogen identification

A process for the identification of genetic material in a biological liquid sample and including the steps of injecting into a reaction vessel containing freeze dried PCR reagents and labelled primers sufficient pure water to liquify the reagents and primers; injecting the biological liquid sample into the reaction vessel; subjecting the reaction vessel contents to a cell disruption process, in the said reaction vessel; conducting pathogen specific polymerase chain reaction (PCR) on the contents of the reaction vessel; and monitoring the PCR and determining therefrom the presence of a specific genetic material. A device for performing the process comprises a heat reduction module (HRM); a peltier cell (TEC) the base plate whereof is contiguous with the HRM; a reaction vessel receiving heat transfer sleeve contiguous with a working face of the TEC; retention means for holding a reaction vessel in the sleeve; and means for driving the TEC.

COMPOSITE LIQUID CELL (CLC) SUPPORTS, AND METHODS OF MAKING AND USING THE SAME

Composite liquid cell supports are provided. Aspects of the supports include: a plurality of CLC containers, wherein each CLC container is configured to hold a CLC and comprises a fluorophilic inner surface having a water contact angle of 80 degrees or greater. The fluorophilic inner surface may have a first contact angle with a fluorous carrier liquid which is less than a second contact angle with an encapsulating liquid that is immiscible with the carrier liquid. The supports find use in, among other applications, CLC systems and devices. Also provided are methods of preparing and using CLC arrays that include the CLC supports of the invention. 1. A composite liquid cell (CLC) support , the support comprising:a plurality of CLC containers, wherein each CLC container is configured to hold a CLC and comprises a fluorophilic inner surface having a water contact angle of 80 degrees or more.2. The support according to claim 1 , wherein the fluorophilic inner surface has a first contact angle with a fluorous carrier liquid; and a second contact angle with an encapsulating liquid that is immiscible with the carrier liquid claim 1 , wherein the first contact angle is less than the second contact angle and the fluorous carrier liquid is more dense than the encapsulating liquid.34-. (canceled)5. The support according to claim 1 , wherein the fluorophilic inner surface comprises a fluoropolymer composition.6. The support according to claim 5 , wherein the fluoropolymer composition comprises a melt processible cofluoropolymer.7. (canceled)8. The support according to claim 5 , wherein the fluoropolymer composition further comprises a pigment or a black colorant to provide for an opaque support.9. The support according to claim 1 , wherein the support has an integrated structure made up of a homogeneous material.10. The support according to claim 1 , wherein the support comprises a multiwell format.11. (canceled)12. The support according to claim 1 , wherein the immiscible ...

SYSTEMS AND METHODS FOR FLUID AND COMPONENT HANDLING

Systems and methods are provided for sample processing. A device may be provided, capable of receiving the sample, and performing one or more of a sample preparation, sample assay, and detection step. The device may be capable of performing multiple assays. The device may comprise one or more modules that may be capable of performing one or more of a sample preparation, sample assay, and detection step. The device may be capable of performing the steps using a small volume of sample. 18-. (canceled)9. A biological sample processing system , comprising:a plurality of sample processing modules;a support structure, wherein said plurality of sample processing modules are mounted on the support structure;a controller; a nucleic acid amplification device configured to perform a nucleic acid assay and without the aid of another module in said system;', 'a mount configured to allow each of the sample processing modules to be detachable from the support structure;, 'wherein each of the sample processing modules compriseswherein each of the sample processing modules is configured to be in electrical, electro-magnetic or optoelectronic communication with the controller, said controller being programmed to provide one or more instructions to, and receive responses from, the module to evaluate indicators of sample or system performancewherein each of the modules is interchangeable with another sample processing module of the system.10. A biological sample processing system , comprising:a plurality of cartridges each containing reagents for a nucleic acid assay;a plurality of sample processing modules, each configured to receive at least one of said cartridges;a support structure, wherein said plurality of sample processing modules are mounted on the support structure;a controller; a nucleic acid amplification device configured to perform the nucleic acid assay and without the aid of another module in said system;', 'a mount configured to allow each of the sample processing ...

Reaction container and biochemical analysis method

A reaction container including a transparent base having a first surface having at least one region where recessed portions are formed and recessed from the first surface, and a cover member positioned such that the cover member forms a gap from the first surface inside the region and is welded to the transparent base outside the region. The cover member absorbs infrared light and transmits light having a wavelength within a range of visible light.

PORTABLE TESTING DEVICE FOR ANALYZING BIOLOGICAL SAMPLES

A portable testing device includes a hosing with an integrated touchscreen display and a receptacle in which a sample holder containing a biological sample and reagent mixture can be placed. The portable testing device further includes an optical assembly positioned in the housing, an electronic assembly that is configured to receive data from the optical assembly and transmit it for display upon the touchscreen display, and a power supply in the housing to power the portable testing device. The optical assembly includes an excitation filter that extends across the entire optical assembly and an emission filter that extends across the entire optical assembly. 1. (canceled)2. A portable testing device comprising:a case comprising a receptacle configured to receive a sample holder containing a biological sample and reagent mixture;an optical assembly positioned in the case, wherein the optical assembly is configured to amplify and detect a signal from the biological sample and reagent mixture in the sample holder, and wherein the optical assembly comprises: a plurality of cavities that are shaped to receive the sample holder;', a first excitation filter;', 'a first emission filter;', 'a first plurality of light-emitting diodes positioned on a side of the first excitation filter opposite the plurality of cavities; and', 'a first plurality of photodetectors positioned on a side of the first emission filter opposite the plurality of cavities; and, 'a first housing portion positioned on a first side of the sample block, wherein the first housing portion comprises, a second excitation filter;', 'a second emission filter;', 'a second plurality of light-emitting diodes positioned on a side of the second excitation filter opposite the plurality of cavities; and', 'a second plurality of photodetectors positioned on a side of the second emission filter opposite the plurality of cavities; and, 'a second housing portion positioned on a second side of the sample block, opposite ...

CARTRIDGE FOR NUCLEIC ACID DETECTION

A cartridge for nucleic acid detection is provided with: a first member having multiple nucleic acid extraction wells; a second member having a nucleic acid amplification and determination well for amplification of a nucleic acid and detection of the amplified nucleic acid; and multiple reagent containers . The multiple reagent containers are fixed between the first member and the second member 1. A cartridge for nucleic acid detection which is used in an apparatus for extracting nucleic acids from a specimen , amplifying the extracted nucleic acids and detecting the amplified nucleic acids , the cartridge comprising:a first member provided with a plurality of nucleic acid extraction wells, the first member comprising at least one nucleic acid extraction well having at least one nucleic acid extraction reagent sealed therein in advance;a second member provided with a nucleic acid amplification/determination well for amplifying the nucleic acids and determining the amplified nucleic acids; andone or a plurality of reagent containers having one or a plurality of nucleic acid amplification reagents sealed therein in advance,wherein the one or the plurality of reagent containers are fixed so as to be a part of the first member and/or the second member, thereby integrating the first member, the second member and the plurality of reagent containers.2. The cartridge for nucleic acid detection according to claim 1 , wherein the cartridge for nucleic acid detection is fixed to the first member and the second member by being sandwiched between the first member and the second member.3. The cartridge for nucleic acid detection according to claim 1 , wherein the plurality of nucleic acid amplification reagent containers are fitted into first openings formed in the first member and/or second openings formed in the second member.4. The cartridge for nucleic acid detection according to claim 3 , wherein:the first member is provided with a first top plate that has a plurality of ...

METHODS AND DEVICES FOR AMPLIFICATION OF NUCLEIC ACID

The present invention relates to methods and devices for amplifying nucleic acid, and, in particular, amplifying so as to generate products on a surface without the use of emulsions. In a preferred embodiment, a plurality of groups of amplified product are generated on the surface, each group positioned in different (typically predetermined) locations on said surface so as to create an array. 16-. (canceled)7. A method of amplifying and sequencing nucleic acid , comprising: i) a population of different nucleic acid template molecules,', 'ii) a plurality of first and second single stranded oligonucleotides immobilized on a glass surface,', 'iii) amplification reagents,', 'iv) sequencing reagents, and', 'v) a plurality of first and second sequencing primers;, 'a) providing'}b) hybridizing at least a portion of said population of nucleic acid template molecules to said plurality of first oligonucleotides immobilized on said glass surface;c) amplifying said nucleic acid template molecules so as to create a plurality of forward strands;d) sequencing said forward oligonucleotide strands with said first sequencing primers;e) amplifying said nucleic acid template molecules so as to create a plurality of reverse single stranded strands; andf) sequencing said reverse oligonucleotide strands with said second sequencing primers.8. The method of claim 7 , wherein said amplification reagents comprise polymerase and dNTPs.9. The method of claim 7 , wherein said sequencing reagents comprise reagents for sequencing by synthesis.10. The method of claim 7 , wherein said plurality of first and second single stranded oligonucleotides are immobilized to said glass surface through a linker.11. The method of claim 10 , wherein said linker elevates said oligonucleotides away from said glass surface.12. The method of claim 7 , wherein when the first sequencing primers are used in step d) claim 7 , said second sequencing primers are not active.1331-. (canceled) The present invention relates ...

Temperature adjustment stage

To provide a temperature adjustment stage that can efficiently heat a cartridge in accordance with necessity of heating each well. A temperature adjustment stage for heating a cartridge in which a plurality of wells that store a liquid are formed. The cartridge is used in an inspection system that detects an object captured by an immunoreaction, the wells include a labeled antibody well that stores a labeled antibody that labels the object, and there is provided a structure that does not heat the labeled antibody well.

Biotip

A biotip according to an embodiment of the invention moves a reaction mixture along a longitudinal direction under the force of gravity. The biotip includes a chamber formed of a transparent material and filled with a liquid having a smaller specific gravity than the reaction mixture and immiscible with the reaction mixture, and a seal that seals the chamber. The liquid has a volume resistivity of greater than 0 Ω·cm and 5×10 13 Ω·cm or less.

Storage system for biological samples in freezing tanks in a liquid/gas nitrogen environment

The storage system is intended to provide users a device, that may securely hold biological samples within a cell in a space saving storage assembly. It is further an aim of the storage system to enable easy retrieval of the biological samples through a simple two-step retrieval action of the cell. Furthermore, the system includes a compact storage assembly that comprises multiple cells and canisters stacked together having efficient structural components that are suited for a typical freezer tank for biological samples. Additionally, the storage system comprises a simple elevator system that enables easy and fast retrieval of a single canister and cell from a large group of canisters. 1. A storage system for storing biological samples in freezer tanks , the system comprising:a plurality of cells;a plurality of canisters;a storage assembly;a locking system;each of the plurality of cells comprising a receptacle and an inner compartment;the inner compartment traversing into the receptacle;the inner compartment traversing from a first end of the receptacle towards a second end of the receptacle, wherein the first end is positioned opposite to the second end across the receptacle;each of the plurality of cells and each of the plurality of canisters being polygonal;a first set of cells being longitudinally mounted within a corresponding canister, wherein the first set of cells are from the plurality of cells, and the corresponding canister is from the plurality of canisters;the locking system being integrated between each of the plurality of cells and the corresponding canister;the plurality of canisters being compactly arranged to form the storage assembly, such that the plurality of canisters are pressed against each other.2. The storage system of claim 1 , wherein each of the plurality of canisters comprising:a sleeve;an inner cavity;a plurality of mounting bases;a pole;the inner cavity traversing into the sleeve;the sleeve partially and perimetrically encompassing ...

OPTICAL DETECTOR

Disclosed herein is an optical detector at least including: a first substrate in which a plurality of wells are formed; a second substrate in which a heating section is provided to heat the wells; a third substrate in which a plurality of photoirradiation sections are provided in alignment with the wells; and a fourth substrate in which a plurality of photodetection sections are provided in alignment with the wells. 1. An optical detecting apparatus configured to detect a signal from a sample in at least one of a plurality of wells , the optical detecting apparatus comprising:heaters configured to control temperatures in the plurality of wells, the heaters including a plurality of pairs of first and second heaters positioned apart to accommodate at least one of the wells in alignment between each respective pair of heaters, the wells being positioned apart from the first and second heaters;light sources configured to illuminate the sample, the light sources being arranged on a first side of the first heaters that is opposed to a well accommodation side of the first heaters; andoptical detection elements configured to detect a signal from the sample, the optical detection elements being arranged on a second side of the second heaters that is opposed to a well accommodation side of the second heaters.2. The optical detecting apparatus of claim 1 ,wherein the heaters include patterned transparent electrodes.3. The optical detecting apparatus of claim 2 ,wherein the transparent electrodes are ITO or ZnO electrodes.4. The optical detecting apparatus of claim 1 ,wherein a plurality of condenser lenses are provided, each of the condenser lenses being provided in alignment between respective opposed light sources and first heaters.5. The optical detecting apparatus of claim 1 ,wherein a plurality of condenser lenses are provided, each of the condenser lenses being provided in alignment between respective opposed optical detection elements and second heaters.6. The optical ...

Systems and Methods for Biological Analysis

A system for performing biological reactions is provided. The system includes a chip including a substrate and a plurality of reaction sites. The plurality of reaction sites are each configured to include a liquid sample of at most one nanoliter. Further, the system includes a control system configured to initiate biological reactions within the liquid samples. The system further includes a detection system configured to detect biological reactions on the chip. According to various embodiments, the chip includes at least 20000 reaction sites. In other embodiments, the chip includes at least 30000 reaction sites. 1. A system for performing biological reactions , the system comprising:a chip including a plurality of reaction sites, wherein the plurality of reaction sites are each configured to include a liquid sample of at most one nanoliter;a control system configured to initiate biological reactions in the liquid samples; anda detection system configured to detect biological reactions on the chip.2. The system of claim 1 , wherein the chip includes at least 20000 reaction sites.3. The system of claim 1 , wherein the chip includes at least 30000 reaction sites.4. The system of claim 1 , wherein each reaction site of the plurality of reaction sites is a through-hole.5. The system of claim 1 , wherein each reaction site of the plurality of reaction sites is a well.6. The system of claim 1 , wherein the control system is configured to perform polymerase chain reaction (PCR).7. The system of claim 6 , wherein the control system is configured to perform digital PCR.8. The system of claim 1 , wherein the detection system includes an optical system claim 1 , wherein the optical system includes an excitation source and an optical sensor configured to detect fluorescent signals emitted from a plurality of liquid samples within the plurality of reaction sites.9. The system of claim 1 , wherein a surface of the reaction sites are hydrophilic.10. The system of claim 9 , wherein ...

System for Conducting the Identification of Bacteria in Biological Samples

The present invention relates to a system for conducting the identification and quantification of micro-organisms, e.g., bacteria in biological samples. More particularly, the invention relates to a system comprising a disposable cartridge and an optical cup or cuvette having a tapered surface; an optics system including an optical reader and a thermal controller; an optical analyzer; a cooling system; and an improved spectrometer. The system may utilize the disposable cartridge in the sample processor and the optical cup or cuvette in the optical analyzer. 128-. (canceled)29. An optical reader for analyzing bacteria in a biological specimen , comprising:an optics cup containing the biological specimen;an illumination arrangement including a xenon light source and a system of turning mirrors, filters and a filter wheel supported in a plurality of carriages for producing an illumination beam;said plurality of carriages being arranged at an angle so as to decrease the distance between the light source and the optics cup and to increase the signal-to-noise ratio of the illumination beam;an anchor shoe for supporting the optics cup and having a slit for producing a collimated beam from the illumination beam and directing the collimated beam into the optics cup containing the biological sample to produce fluorescent emissions; andan optical collection device for receiving the fluorescent emissions from the biological specimen and the optics cup and directing the fluorescent emissions to a detection device for the analysis of bacteria in the biological specimen.30. The optical reader of claim 29 , wherein the filter wheel includes a plurality of narrowband filters and wherein the filter wheel is selectively employed to change the narrowband filters so that the radiation from the light source is restricted to a narrow band of ultraviolet light ranging from 260 nm to about 300 nm.31. The optical reader of claim 29 , wherein the optics cup includes a rectangular-shaped ...

MULTIPLEX SLIDE PLATE DEVICE AND OPERATION METHOD THEREOF

A multiplex slide plate device and an operation method thereof are provided. The multiplex slide plate device includes a slide plate and a sacrificial layer. The slide plate has reaction vessels arranged in an array, an injection hole and an exhaust hole, wherein each of the reaction vessels has an opening portion and a bottom portion. The sacrificial layer has a microfluidic channel, wherein the microfluidic channel has an injection channel, a main channel and a distal channel connected to each other. The sacrificial layer is assembled to the slide plate, wherein the main channel faces the opening portion. A sample solution is injected into the injection channel, such that the sample solution flows from the injection channel through the main channel to the distal channel, wherein the sample solution loads into each of the reaction vessels while flowing through the main channel. 1. A multiplex slide plate device , comprising:a slide plate having a plurality of reaction vessels, a first injection hole and a first exhaust hole, wherein the reaction vessels are arranged in an array, and each of the reaction vessels has an opening portion and a bottom portion; anda sacrificial layer having a microfluidic channel, wherein the microfluidic channel has an injection channel, a main channel and a distal channel connected to each other, and the sacrificial layer is assembled to the slide plate, wherein the main channel faces the opening portion;wherein a sample solution is injected from the first injection hole to the injection channel, such that the sample solution flows from the injection channel through the main channel to the distal channel, and the sample solution loads into each of the reaction vessels while flowing through the main channel.2. The multiplex slide plate device of claim 1 , further comprising a housing which accommodates the slide plate and the sacrificial layer claim 1 , and the housing has a second injection hole and a second exhaust hole claim 1 , ...

LED DRIVEN PLASMONIC HEATING APPARATUS FOR NUCLEIC ACIDS AMPLIFICATION

Systems and methods for plasmonic heating by combined use of thin plasmonic film-based 2D and 3D structures and a light-emitting diode (LED) for nucleic acids amplification through fast thermal cycling of polymerase chain reaction (PCR) are described. 1. An apparatus for nucleic acids amplification , comprising:a support platform comprising one or more wells configured to hold a sample;a plasmonic thin film disposed within the one or more wells; anda light source;wherein the light source is configured to be directed at the support platform such that exposed light from the light source to generates plasmonic photothermal light-to-heat conversion within the plasmonic thin film and subsequent heating of the sample.2. The apparatus of claim 1 , further comprising:a lens disposed between the light source and the support platform;wherein the lens is configured to focus the exposed light at the one or more wells.3. The apparatus of claim 1 , further comprising a temperature sensor configured to monitor the temperature of the sample.4. The apparatus of claim 3 , further comprising:a controller coupled to one or more of the light source and temperature sensor;the controller configured for controlling one or more of data acquisition from the temperature sensor and actuation of the light source.5. The apparatus of claim 4 , wherein the controller is configured for controlling actuation of the light source to modify one or more of exposure duration and injection current at the plasmonic thin film.6. The apparatus of claim 1 , wherein the plasmonic thin film comprises a nanometer sized grain to enhance light absorption through surface plasmon resonance.7. The apparatus of claim 1 , wherein the support platform comprises a translucent or transparent polymer.8. The apparatus of claim 3 , wherein the temperature sensor comprises a long wavelength infrared (LWIR) camera oriented adjacent to the sample.9. The apparatus of claim 2 , further comprising a diffuser associated with the ...

SELF-DIGITIZATION OF SAMPLE VOLUMES

Devices, systems and apparatuses for the discretization and manipulation of sample volumes are provided. Related methods are also provided. 1. A microfluidic device comprising:a microwell plate comprising a plurality of wells;a fluid inlet port in fluidic communication with the well;a fluid outlet port in fluidic communication with the well;a flow channel having a flow axis, the flow channel in fluidic communication with the fluid inlet port and the fluid outlet port; anda plurality of fluidic harbors in fluidic communication with the flow channel and offset from the flow axis.2. The microfluidic device of claim 1 , wherein at least one well of the plurality of wells comprises a plurality of the flow channels.3. The microfluidic device of claim 1 , wherein at least one well of the plurality of the wells comprises a plurality of fluid inlet ports including the fluid inlet port.4. The microfluidic device of claim 1 , wherein at least one well of the plurality of the wells comprises a plurality of fluid outlet ports including the fluid outlet port.5. The microfluidic device of claim 1 , wherein the plurality of wells is arranged in an array.6. The microfluidic device of claim 1 , wherein the plurality of wells is arranged in a square matrix array.7. The microfluidic device of claim 1 , wherein the plurality of wells comprises 6 claim 1 , 12 claim 1 , 24 claim 1 , 48 claim 1 , 96 claim 1 , 384 claim 1 , or 1536 wells.8. The microfluidic device of claim 1 , wherein at least one fluidic harbor of the plurality of fluidic harbors is at an angle other than orthogonal to the flow axis.9. The microfluidic device of claim 1 , wherein at least one fluidic harbor of the plurality of fluidic harbors is at an angle orthogonal to the flow axis.10. The microfluidic device of claim 1 , wherein each fluidic harbor of the plurality of fluidic harbors is in fluidic communication with the flow channel by an opening conduit.11. The microfluidic device of claim 1 , wherein at least one ...

PURIFICATION AND DETECTION OF ANALYTES

A sample preparation module accepts a sample including a target analyte. The sample preparation module processes the sample through several reaction chambers and a solid phase column. Different reagents are present in the reaction chambers. The eluted analyte is then transferred to the amplification module, where it is further processed and amplified for optical analysis. 1. (canceled)3. The device of claim 2 , wherein a bottom part of each reaction well of the first plurality of reaction wells and of the second plurality of reaction wells is transparent to light claim 2 , thereby allowing optical analysis of liquids within the first plurality of reaction wells and within the second plurality of reaction wells.4. The device of claim 2 , wherein each reaction well of the first plurality of reaction wells and of the second plurality of reaction wells comprises lyophilized reagents.5. The device of claim 2 , wherein each reaction well of the first plurality of reaction wells and of the second plurality of reaction wells comprises a hydrophobic membrane configured to allow through passage of air.6. The device of claim 3 , further comprising a heater configured to control a temperature of the first plurality of reaction wells and of the second plurality of reaction wells.7. A system comprising the device of and a fluorescence imager configured to measure fluorescence of the first plurality of reaction wells and of the second plurality of reaction wells. The present application is a continuation application of U.S. application Ser. No. 16/130,810 filed on Sep. 13, 2018, which in turn, claims priority to U.S. Provisional Patent Application No. 62/558,679, filed on Sep. 14, 2017, and may be related to U.S. Pat. No. 9,822,356, issued on Nov. 21, 2017, U.S. Pat. No. 9,561,505, issued on Feb. 7, 2017, U.S. Pat. No. 9,518,291, issued on Dec. 13, 2016, U.S. Pat. No. 9,803,237, issued on Oct. 31, 2017, U.S. Pat. No. 9,415,392, issued on Aug. 16, 2016, and US Patent Application ...

Thermal cycler

A biotip according to an embodiment of the invention moves a reaction mixture along a longitudinal direction under the force of gravity. The biotip includes a chamber formed of a transparent material and filled with a liquid having a smaller specific gravity than the reaction mixture and immiscible with the reaction mixture, and a seal that seals the chamber. The liquid has a volume resistivity of greater than 0 Ω·cm and 5×10 13 ∩·cm or less.

REACTION PROCESSOR

A reaction processor is provided with a reaction processing vessel in which a channel is formed, a liquid feeding system, a temperature control system for providing a high temperature region and a low temperature region to the channel, and a fluorescence detector for detecting the sample passing through a fluorescence detection region of the channel, and a CPU for controlling the liquid feeding system based on a signal that is detected. A target stop position X(n+1) of the sample in the low temperature region in an (n+1)th cycle is corrected from a target stop position X(n) of the sample in the low temperature region in the nth cycle based on the result of stopping control on the sample in the nth cycle. 1. A reaction processor comprising:a reaction processing vessel in which a channel where a sample moves is formed;a liquid feeding system that moves and stops the sample in the channel;a temperature control system that provides a plurality of temperature regions each maintained at a different temperature, the plurality of temperature regions including at least a first temperature region and a second temperature region;a detection system that detects the sample passing through a detection region that is set between adjacent temperature regions of the channel, the detection region including at least a first detection region that is set between the first temperature region and the second temperature region of the channel; anda control unit that controls the liquid feeding system based on a signal detected by the detection system,wherein the control unit is formed to perform reciprocation control for a plurality of cycles on the sample where, in one cycle, the sample is stopped for a fixed period of time in the first temperature region, moves from the first temperature region to the second temperature region after passing through the first detection region and is then stopped for a fixed period of time, then returns to the first temperature region and then stops,wherein ...

SYSTEMS AND METHODS FOR BIOLOGICAL ANALYSIS

A system for processing a plurality of biological samples contains a support and a temperature controller. The support is configured to hold a case that includes an inner chamber and a substrate located within the inner chamber, the substrate containing a plurality of isolated reaction sites containing one or more biological samples. The temperature controller is configured to maintain or control a temperature of at least one of the support, the case, or the one or more biological samples during an assay or reaction on the one or more biological samples. The support is also configured to maintain at least one of the surfaces of substrate at a positive angle relative to a horizontal plane during the assay or reaction. 1. A system for processing a plurality of biological samples , comprising: a base;', 'a cover disposed over at least a portion of the base, the base and the cover together forming an inner chamber;', 'a substrate located within the inner chamber, the substrate comprising a first surface, an opposing second surface opposite the first surface, and a plurality of through-holes disposed between the surfaces, the plurality of through-holes containing one or more biological samples; and', 'a sealing fluid disposed within the inner chamber and covering the surfaces of the substrate; and, 'a support configured to hold a case comprisinga temperature controller configured to maintain or control a temperature of at least one of the support, the case, the base, the substrate, the cover, the sealing fluid, or the one or more biological samples during an assay or reaction on the one or more biological samples;wherein the support is configured to maintain at least one of the surfaces of substrate at a positive angle relative to a horizontal plane during the assay or reaction.28-. (canceled)9. A method of processing a plurality of biological samples , comprising: a base;', 'a cover disposed over at least a portion of the base, the base and the cover together forming an ...

CONTAINER FOR BIOLOGICAL PRESERVATION AT LOW TEMPERATURE

A container is provided in the present disclosure. The container includes at least one outer wall that is made of a low temperature tolerant material where the at least one outer wall is arranged to substantially form the container with an opening end; a tube that is situated inside the at least one outer wall, where the tube and the at least one outer wall are substantially thermally insulated, and the tube is open at one end that is on a same side of the opening end of the container; and a container cover being shaped substantially to match the at least one outer wall, where the container over is capable of covering the opening end of the container. 1. A container , comprising:at least one outer wall that is made of a low temperature tolerant material, wherein the at least one outer wall is arranged to substantially form the container with an opening end;a tube that is situated inside the at least one outer wall, wherein the tube and the at least one outer wall are substantially thermally insulated, and the tube is open at one end that is on a same side of the opening end of the container; anda container cover being shaped substantially to match the at least one outer wall, wherein the container over is capable of covering the opening end of the container, and when the opening end of the container is covered by the container cover, the container cover substantially seals the tube and substantially thermally insulate the tube such that the container is capable of being placed in a low temperature environment.2. The container of claim 1 , wherein the low temperature environment has a temperature that is below or equal to −80° C.3. The container of claim 1 , wherein the at least one outer wall is substantially squarely shaped by four side walls claim 1 , and each of the four side walls is shaped substantially rectangularly and has about a same size.4. The container of claim 3 , wherein more than one containers are capable of bundling together to form a substantially ...

INSTRUMENT AND METHOD FOR THE AUTOMATED THERMAL TREATMENT OF LIQUID SAMPLES

An instrument and a method for the automated thermal treatment of liquid samples are disclosed. An inter-distance between a temperature-controlled receptacle for loading with a plurality of vessels for containing the samples and end portions of optical fibers can be varied, wherein the receptacle is configured to form a thermal communication with the loaded vessels and wherein the optical fibers have first and second end portions. The first end portion and the second end portion of each optical fiber is fixed with respect to each other for transmitting light, wherein the variation of the in-ter-distance allows the vessels to be loaded to or unloaded from the receptacle and to enable detection of light from the samples contained in the one or more receptacle-loaded vessels. 1. An instrument for the automated thermal treatment of liquid samples , comprising:a temperature-controlled receptacle for loading with a plurality of vessels for containing said samples, said receptacle being configured to form a thermal communication with said loaded vessels;a detection module equipped with a detection arrangement provided with one or more detectors for detecting light emitted from said samples and a coupling arrangement provided with a plurality of optical fibers for transmitting said emitted light to said detection arrangement, wherein said optical fibers have first and second end portions, said firs tend portion and said second end portion of each optical fiber being fixed with respect to each other;a moving mechanism for moving said coupling arrangement and/or said receptacle in a manner to vary an inter-distance between said coupling arrangement and said receptacle so as to allow the vessels to be loaded to or unloaded from said receptacle and to allow detection of light from samples contained in said one or more receptacle-loaded vessels.2. The instrument of claim 1 , wherein the moving mechanism is configured to move the coupling arrangement while the receptacle is being ...

PURIFICATION AND DETECTION OF ANALYTES

A sample preparation module accepts a sample including a target analyte. The sample preparation module processes the sample through several reaction chambers and a solid phase column. Different reagents are present in the reaction chambers. The eluted analyte is then transferred to the amplification module, where it is further processed and amplified for optical analysis. 1. A device comprising:a plurality of chambers, each chamber of the plurality of chambers comprising a top opening, a bottom opening, and a seal; seal the plurality of chambers by contacting the seal of each chamber of the plurality of chambers, and', 'upon pushing of the lid towards the plurality of chambers, simultaneously pressurize each chamber of the plurality of chambers;, 'a lid configured toa storage section comprising a plurality of receptacles; anda rotating section between the plurality of chambers and the storage section, the rotating section comprising a solid phase column, wherein the rotating section is configured to rotate to form, in sequence, a fluidic pathway between the solid phase column, the bottom opening of each chamber of the plurality of chambers, and a receptacle of the plurality of receptacles, thereby allowing flow of a pressurized fluid from a chamber of the plurality of chambers through the solid phase column into a receptacle of the plurality of receptacles.2. The device of claim 1 , wherein:at least one chamber of the plurality of chambers comprises a pierceable membrane configured to contain a fluid within the at least one chamber,the lid comprises at least one piercer, andthe lid is further configured to, upon pushing of the lid towards the plurality of chambers, pierce, by the at least one piercer, through the pierceable membrane of the at least one chamber.3. The device of claim 1 , wherein:at least one chamber of the plurality of chambers comprises a pressure-activated membrane configured to contain a fluid within the at least one chamber, andthe pressure- ...

Automated isolation and chemical reaction(s) of nucleic acids

The present teachings relate to methods, kits and devices for performing automated sequential nucleic acid isolation and conversion/purification in a single closed system. In various embodiments, the present teaching enable a user to (i) load a device with test samples, reagents and consumables; (ii) select or program the device for the desired nucleic acid isolation and subsequent chemical treatment and/or conversion reaction(s) without further user intervention; and recovering the isolated and treated and/or converted nucleic acid at the conclusion of the program once the device is activated.

OPTICAL DETECTOR

Disclosed herein is an optical detector at least including: a first substrate in which a plurality of wells are formed; a second substrate in which a heating section is provided to heat the wells; a third substrate in which a plurality of photoirradiation sections are provided in alignment with the wells; and a fourth substrate in which a plurality of photodetection sections are provided in alignment with the wells. 1. An optical detector comprising:a first substrate in which a plurality of wells are formed;a second substrate in which a heating means is provided to heat the wells;a third substrate in which a plurality of photoirradiation means are provided in alignment with the wells; anda fourth substrate in which a plurality of photodetection means are provided in alignment with the wells.2. The optical detector of claim 1 , whereinthe plurality of heating means are provided in the second substrate in alignment with the wells.3. The optical detector of claim 1 , whereinthe heating means include transparent electrodes patterned in the second substrate.4. The optical detector of claim 3 , wherein the transparent electrodes are ITO or ZnO electrodes.5. The optical detector of claim 1 , whereinthe second substrate is stacked on the side of the first substrate facing the third substrate.6. The optical detector of claim 1 , whereinthe second substrates are stacked one on each side of the first substrate in such a manner as to sandwich the first substrate.7. The optical detector of claim 1 , whereina plurality of condenser lenses are provided between the photoirradiation means and wells.8. The optical detector of claim 1 , whereina plurality of condenser lenses are provided between the wells and photodetection means.9. The optical detector of serving as a nucleic acid amplification detector capable of detecting nucleic acid amplification in the wells.10. The optical detector of claim 9 , whereinan isothermal amplification method is used for the nucleic acid amplification ...

APPARATUS TO FACILITATE TRANSFER OF BIOLOGICAL SPECIMENS STORED AT CRYOGENIC CONDITIONS

A system and method facilitates transfers of specimen containers (e.g., vials with caps) between storage cassettes and carrier cassettes. The storage cassettes are designed to be stored in cryogenic refrigerators while the carrier cassettes are designed to be temporarily stored in a portable carrier. Identification information is read from wireless transponders carried by the specimen containers. Visual mappings of the positions of the specimen container in the cassettes is provided. Presence and position of the specimen containers in the cassettes is verified, and alerts of inconsistencies provided along with corrective commands. Inventories of specimen container and even specific specimen holders are provided. 1. An interrogation system operable to interrogate wireless transponders physically associated with respective specimen containers , the wireless transponder and the respective specimen containers stored in a cryogenic environment , the interrogation system comprising:a plurality of antennas spatially arrayed in an array of antennas, the array of antennas having a set of one or more dimensions;at least one radio communicatively coupled to drive the antennas to emit interrogation signals to interrogate the wireless transponders in the cryogenic environment and to receive response signals from any of the wireless transponders in a range of one or more of the antennas; anda processor-based control system communicatively coupled to the at least one radio, the processor-based control system which, for each response signal received in response to a given interrogation, determines which response signal was returned from one of the wireless transponders that is closest wireless transponder to the antenna that emitted the respective interrogation signal to which the wireless transponders are responding, and which stores in at least one data structure a mapping that relates an identity of at least one of the wireless transponders and a respective spatial position in ...

TEMPERING BLOCK MODULE AND APPARATUS FOR THE THERMAL TREATMENT OF SAMPLES

The present disclosure relates to a tempering block module for the thermal treatment of samples, comprising: a tempering block; and an ejection mechanism for lifting reaction vessels off the tempering block, the ejection mechanism including first and second ejection plungers that are movably mounted in the tempering block module perpendicular to the tempering block from a first position, retracted into the tempering block module, into a second position, extended out of the tempering block module, and wherein the tempering block module includes a first plunger drive connected to the first ejection plunger for driving the movement of the first ejection plunger and a second plunger drive, different from the first plunger drive, connected to the second ejection plunger for driving the movement of the second ejection plunger from the first to the second position or from the second to the first position. 1. A tempering block module for a device for thermal treatment of samples , the tempering block module comprising:a tempering block;an ejection mechanism configured to lift reaction vessels disposed on the tempering block from the tempering block and including a first ejection plunger and a second ejection plunger, wherein the first ejection plunger and the second ejection plunger are movably mounted in the tempering block module perpendicular to a plane in which the tempering block is arranged such that the first ejection plunger and the second ejection plunger are movable from a retracted first position within the tempering block module to a second position extended out of the tempering block module;a first plunger drive operatively connected to the first ejection plunger and configured to drive movement of the first ejection plunger from the first position to the second position or from the second position to the first position; anda second plunger drive operatively connected to the second ejection plunger and configured to drive movement of the second ejection plunger ...

Reduced Dimensionality Structured Illumination Microscopy With Patterned Arrays of Nanowells

Techniques are described for reducing the number of angles needed in structured illumination imaging of biological samples through the use of patterned flowcells, where nanowells of the patterned flowcells are arranged in, e.g., a square array, or an asymmetrical array. Accordingly, the number of images needed to resolve details of the biological samples is reduced. Techniques are also described for combining structured illumination imaging with line scanning using the patterned flowcells.

HANDLING LIQUID SAMPLES

Devices, systems and methods for making and handling liquid samples are disclosed. 1. A capillary system comprising: the outer surface of the capillary tube is substantially hydrophobic; and', 'the inner surface of the capillary tube includes a metering region that is substantially hydrophilic and adjacent to the distal end of the tube;, 'at least one capillary tube having an inner surface and an outer surface, the outer surface including an exterior wall of the capillary tube, a proximal end of the capillary tube and a distal end of the capillary tube, whereina pressure source in fluid communication with the proximal end of the capillary tube; 'an air sheath that applies an external air flow to the capillary tube.', 'a capillary controller operably connected to the pressure source, the capillary controller programmed to cause the pressure source to apply sufficient positive pressure to the proximal end of the capillary tube to drive an aqueous solution out of the distal end of the capillary tube; and'}2. (canceled)3. The capillary system of wherein the metering region comprises a substantially hydrophilic etched fluoropolymer and the limiting region and outer surface both comprise a substantially hydrophobic fluoropolymer.4. The capillary system of wherein the length and cross-sectional area of the metering region define a predetermined volume.5. The capillary system of wherein the capillary tube comprises a glass substrate.6. The capillary system of wherein the metering region comprises an exposed hydrophilic surface of the glass substrate.7. The capillary system of wherein:the capillary tube comprises a glass substrate; andthe metering region comprises an exposed hydrophilic surface of the glass substrate.8. The capillary system of further comprising an actuator configured to move the capillary tube between locations claim 1 , the actuator being operably connected to the capillary controller; and wherein the capillary controller is programmed to:cause the ...

High Speed Nucleic Acid Melting Analysis

A method and system have been provided to perform high speed nucleic acid melting analysis while still obtaining accurate melting curve sufficient for genotyping. This rapid ability to interrogate DNA should be useful whenever time to result is important, such as in molecular point of care testing. Specifically, microfluidics enables genotyping by melting analysis at rates up to 50° C./s, requiring less than is to acquire an entire melting curve. High speed melting reduces the time for melting analysis, decreases errors, and improves genotype discrimination of small amplicons. 1. A method for performing a nucleic acid high speed melting analysis , the method comprising:providing a microfluidic device having one or more nucleic acid samples therein;providing an imaging system in communication with the one or more nucleic acid samples;providing a thermal system in thermal communication with the one or more nucleic acid samples;increasing a temperature of the one or more nucleic acid samples to achieve nucleic acid dissociation, wherein the temperature is increased by the thermal system at a ramp rate selected from a range of 1° C./s to 50° C./s.acquiring images of the one or more nucleic acid samples during nucleic acid dissociation to generate a melting profile for each nucleic acid sample; andgenotyping the nucleic acids based on the melting profiles.2. The method of claim 1 , wherein the microfluidic device comprises a microfluidic cartridge and a reaction chip.3. The method of claim 2 , wherein the reaction chip comprises one or more microchannels.4. The method of claim 3 , wherein the melting analysis is performed when the one or more samples are in the one or more microchannels of the reaction chip.5. The method of claim 1 , wherein the nucleic acid melting analysis is performed by increasing a temperature of the one or more nucleic acid samples at the rate selected from the range of 1° C./s to 8° C./s.6. The method of claim 1 , wherein the nucleic acid melting ...

Apparatus to preserve and identify biological samples at cryogenic conditions

A container includes a vial, cap, and one or more wireless transponders secured to the cap, the vial or a jacket to store and identify samples of biological material at cryogenic temperatures (e.g., vitrified biological samples), for instance held by cryopreservation storage devices. A specimen holder may be extend from the cap. The vial and/or cap includes ports or vents. A carrier includes a box, thermal shunt, thermal insulation to store and identify arrays of containers that hold cryopreservation storage devices with samples of biological material at cryogenic temperatures. Various apparatus include wireless transponders positioned and oriented to enhance range, and allow interrogation while retained in a carrier. Various apparatus can maintain the biological material at or close to cryogenic temperatures for prolonged period of times after being removed from a cryogenic cooler, and can allow wireless inventorying while maintaining the biological samples at suitably cold temperatures.

CONTAINER SYSTEM FOR TRANSPORT OF BIOLOGICAL MATERIAL, USES THEREOF AND METHODS OF PACKAGING

The invention relates to a container system for transport of biological material comprising a container casing and an insulating device, wherein the container casing defines a container interior space for accommodation of the insulating device and for accommodation of biological material to be transported, and wherein the insulating device is constituted of at least two insulating portions, which are formed so as to surround the biological material inside the container interior space. The invention further relates to uses of such a container system for packaging and transport of biological material, as well as methods of packaging biological material for transport. 1. A container system for transport of biological material comprising:a container casing formed of a temperature-insulating plastic, andan insulating device,wherein the container casing defines a container interior space for accommodation of the insulating device and for accommodation of biological material to be transported,and wherein the insulating device comprises at least two insulating portions, which are formed so as to surround the biological material inside the container interior space, wherein the insulating device and/or the at least two insulating portions is/are formed as a temperature-insulating gel cushion, andwherein the at least two insulating portions are connected to each other and/or are formed integrally with each other, and wherein the at least two insulating portions are foldable relative to each other along an interruption section.2. (canceled)3. The container system according to claim 1 , wherein the insulating portions are each formed by at least one gel chamber claim 1 , and/or wherein the at least two insulating portions are each positionable along a plane claim 1 , and/or wherein the interruption section separates two gel chambers adjacent to each other claim 1 , and/or wherein the interruption section is unfilled or has at least a lower filling degree than at least one of the ...

Palette-based systems for analyte characterization

Systems, devices, and methods are provided that facilitate synthesis and analysis of chemical compounds on a convenient and automatable palette-based platform. Palettes utilized for analysis support a variety of functional sites, including test wells, separation devices, and test sites that have rapid thermal equilibration, on a single test fixture. This permits both the performance of a wide variety of tests and of tests with different temperature requirements or responses in parallel, on the same test fixture. Such systems, devices, and methods are particularly suitable for production and characterization of radiopharmaceuticals.

BENCHTOP NUCLEIC ACID LIBRARY PREPARATION DEVICE AND METHODS FOR USING THE SAME

Complete nucleic acid library preparation devices are provided. Aspects of the devices include: a thermal chip module comprising multiple CLC reaction wells; one or more plate locations; a robotically controlled liquid handler configured to transfer liquid between the one or more plate locations and the thermal chip module; and a bulk reagent dispenser configured to access each CLC reaction well of the thermal chip module. 1. A complete bench-top nucleic acid library preparation device , the device comprising:a thermal chip module configured to receive a composite liquid cell (CLC) reaction cartridge;a consumable reagent location configured to receive a CLC nucleic acid library preparation reagent cartridge;a sample location configured to receive a CLC sample cartridge; anda robotically controlled liquid handler configured to transfer liquid between the consumable reagent location, the sample location, and the thermal chip module.2. The device according to claim 1 , further comprising a magnetic nucleic acid library purification system.3. The device according to claim 2 , further comprising a magnetic bead trough.4. The device according to claim 1 , wherein the device comprises a mechanically actuated lid for the thermal chip module.5. The device according to claim 1 , wherein the CLC nucleic acid library preparation reagent cartridge comprises from 1 to 50 CLC nucleic acid library reagent columns claim 1 , wherein each column comprise a set of nucleic acid library reagents used to prepare a nucleic acid library from a single sample.6. The device according to claim 5 , wherein each CLC nucleic acid library reagent column comprises from 1 to 30 CLC nucleic acid library reagent wells.7. The device according to claim 1 , wherein the CLC sample cartridge comprises a well for receiving a sample provided by a user and a well comprising a CLC nucleic acid barcoding reagent.8. The device according to claim 1 , wherein the consumable reagent location and the sample location ...

POLYCARBONATE MICROFLUIDIC ARTICLES

Microfluidic devices, and methods for their use are described. The microfluidic devices include articles formed from a thermoplastic composition comprising a poly(aliphatic ester)-polycarbonate comprising soft block ester units, derived from monomers comprising an alpha, omega Caliphatic dicarboxylic acid or derivative thereof, a dihydroxyaromatic compound, and a carbonate source. 1. A method of using a microfluidic device for processing fluids , comprising exposing a portion of the device to a processing temperature of at least 90° C.;wherein the portion of the device exposed to said processing temperature includes an article formed from a thermoplastic composition comprising:a poly(aliphatic ester)-polycarbonate comprising soft block ester units, derived from monomers comprising:{'sub': '6-20', 'an alpha, omega Caliphatic dicarboxylic acid or derivative thereof,'}a dihydroxyaromatic compound, anda carbonate source.2. The method of claim 1 , wherein the alpha claim 1 , omega Caliphatic dicarboxylic acid or derivative thereof comprises sebacic acid.3. The method of claim 1 , wherein the article comprises a section having a fabricated dimension of less than or equal to 1 mm.4. The method of claim 3 , wherein the fabricated dimension is 0.005 to 1 mm.5. The method of claim 3 , wherein the fabricated dimension is 0.01 to 0.5 mm.6. The method of claim 3 , wherein the fabricated dimension is 0.05 to 0.2 mm.9. The method of claim 7 , wherein m is 8.10. The method of claim 1 , wherein the thermoplastic composition has an MVR of 66 to 150 g/10 min measured at 300° C. and under a load of 1.2 kilograms according to ASTM D1238-10.11. The method of claim 1 , wherein the thermoplastic composition has an HDT is 80 to 140° C. measured at 0.45 mega Pascal (MPa) using unannealed 3.2 mm plaques according to ASTM D648-07.12. The method of claim 11 , wherein the thermoplastic composition has a Notched Izod Impact (NII) ductility of 30 to 100% claim 11 , measured at 23° C. using ⅛-inch ...

ANALYSIS INSTRUMENT

An apparatus for moving a sample holder on a platform from a loading position, where the sample holder can be removed from the platform , to a locked position, where the sample holder is securely held, wherein the sample holder rests on wheels within a recessed portion on the platform , wherein the apparatus is configured such that movement from the loading position to the locked position causes a vertical clamping means to lower down on top of the sample holder , and a horizontal clamping means to be pressed to the outer periphery of the sample holder , and such that that movement from the locked position to the loading position causes the vertical clamping means to raise above the sample holder , and the horizontal clamping means to be moved away from the outer periphery of the sample holder 1. An apparatus for moving a sample holder on a platform from a loading position , where the sample holder can be removed from the platform , to a locked position , where the sample holder is securely held ,wherein the sample holder rests on wheels within a recessed portion on the platform,wherein the apparatus is configured such that movement of the platform from the loading position to the locked position causes a vertical clamping means to lower down on top of the sample holder, and a horizontal clamping means to be pressed to the outer periphery of the sample holder, and such that that movement of the platform from the locked position to the loading position causes the vertical clamping means to raise above the sample holder, and the horizontal clamping means to be moved away from the outer periphery of the sample holder.2. An apparatus according to claim 1 , wherein the vertical clamping means comprises a platform lid; optionally wherein in the locked position claim 1 , the platform lid lies parallel to the platform.3. An apparatus according to claim 2 , wherein the platform lid comprises a first end which is hingedly attached to the platform claim 2 , and a second end ...

AUTOMATED ISOLATION AND CHEMICAL REACTION(S) OF NUCLEIC ACIDS

The present teachings relate to methods, kits and devices for performing automated sequential nucleic acid isolation and conversion/purification in a single closed system. In various embodiments, the present teaching enable a user to (i) load a device with test samples, reagents and consumables; (ii) select or program the device for the desired nucleic acid isolation and subsequent chemical treatment and/or conversion reaction(s) without further user intervention; and recovering the isolated and treated and/or converted nucleic acid at the conclusion of the program once the device is activated. 1. A method for performing sequential nucleic acid isolation and conversion/purification reaction(s) in a single closed system , wherein an isolated nucleic acid is prepared for modification detection , comprising:a. reacting the nucleic acid-containing sample with an extraction reagent, wherein the volume of the sample is ranging from 2 to 4 ml, wherein an isolated nucleic acid is obtained from the sample, andb. reacting said isolated nucleic acid with a conversion reagent, wherein the volume is automatically reduced to 50-250 ul wherein the said nucleic acid having the modification is prepared for further detection.2. The method of claim 1 , wherein said modification is methylation.3. The method of claim 2 , wherein the single closed system is built in an automated device.4. The method of claim 3 , wherein the single closed system can be a sterile environment.5. The method of claim 1 , wherein the conversion is a chemical conversion.6. The method of claim 5 , wherein the chemical conversion is bisulfite conversion with an optional desulfonation step claim 5 , and wherein reagents have an over-layer of mineral oil.7. The method of claim 6 , wherein the bisulfite conversion is a deamination of cytosine into uracil of said isolated nucleic acid.8. The method of claim 1 , wherein the isolation and conversion/purification of the nucleic acid occurs in less than about three and ...

MULTI VESSEL RING

A multi vessel ring (suitable for use with thermal cycler and PCR apparatus) comprises a ring body and a plurality of elongate tubes. Each tube has a proximal open end and a distal closed end, each elongate tube being integrally formed with the ring body, and being pivotally connected to the ring body between an initial position in which a longitudinal axis of each tube is generally parallel with an axis of rotation of the ring body, and a final position in which the longitudinal axis of each tube is inclined relative to the axis of rotation of the ring body. The multi vessel ring preferably further comprises a plurality of caps integrally formed with the ring body, and adapted to seal the proximal open end of respective tubes. A further embodiment is directed to an assembly comprising said ring and a carrier disc having a central hub adapted to be mounted to a thermal cycler, a circumferentially outer portion of the carrier disc having a plurality of grooves, each groove being adapted to support one of said tubes when the multi-vessel ring is located on the carrier disc. A yet further embodiment is directed to a capping tool adapted for use with such an assembly, comprising a loading block having a plurality of projections, each projection being insertable within an aperture formed in an underside of the ring body to push one said cap body from an initial position, in which the cap body is generally coplanar with the ring body, to an intermediate position in which the cap body has rotated more than 90 degrees relative to the ring body. 1. A multi vessel ring comprising:a ring body;a plurality of elongate tubes, each tube having a proximal open end and a distal closed end, each elongate tube being integrally formed with the ring body, and being pivotally connected to the ring body between an initial position in which a longitudinal axis of each tube is generally parallel with an axis of rotation of the ring body, and a final position in which the longitudinal axis ...

Biological Analysis Systems, Devices, And Methods

A device for performing biological sample reactions may include a plurality of flow cells configured to be mounted to a common microscope translation stage, wherein each flow cell is configured to receive at least one sample holder containing biological sample. Each flow cell also may be configured to be selectively placed in an open position for positioning the at least one sample holder into the flow cell and a closed position for reacting biological sample contained in the at least one sample holder. The plurality of flow cells may be configured to be selectively placed in the open position and the closed position independently of each other. 1. A device for performing biological sample reactions , the device comprising:a plurality of flow cells configured to be removably mounted relative to a microscope translation stage;wherein each of the plurality of flow cells is configured to receive a sample holder containing at least one biological sample,wherein each of the plurality of flow cells is further configured to receive fluid when mounted relative to the microscope translation stage to enable a biochemical reaction of the at least one biological sample.2. The device of claim 1 , further comprising a frame mounted to the microscope translation stage claim 1 , wherein the plurality of flow cells are configured to be removably mounted to the frame.3. The device of claim 1 , further comprising a thermal system configured to control a temperature of the at least one biological sample received by the plurality of flow cells.4. The device of claim 1 , wherein the thermal system comprises at least one forced air cooling component.5. The device of claim 1 , wherein the thermal system comprises at least one thermoelectric component.6. The device of claim 1 , wherein each of the plurality of flow cells is configured to be mounted relative to a benchtop fixture to receive the sample holder.7. The device of claim 1 , wherein each of the plurality of flow cell is configured ...

SYSTEM AND METHOD FOR PROCESSING AND DETECTING NUCLEIC ACIDS

A system and method for processing and detecting nucleic acids from a set of biological samples, comprising: a capture plate and a capture plate module configured to facilitate binding of nucleic acids within the set of biological samples to magnetic beads; a molecular diagnostic module configured to receive nucleic acids bound to magnetic beads, isolate nucleic acids, and analyze nucleic acids, comprising a cartridge receiving module, a heating/cooling subsystem and a magnet configured to facilitate isolation of nucleic acids, a valve actuation subsystem configured to control fluid flow through a microfluidic cartridge for processing nucleic acids, and an optical subsystem for analysis of nucleic acids; a fluid handling system configured to deliver samples and reagents to components of the system to facilitate molecular diagnostic protocols; and an assay strip configured to combine nucleic acid samples with molecular diagnostic reagents for analysis of nucleic acids. 1. A system for processing nucleic acid content of a sample in cooperation with a cartridge comprising a heating region defined at a first surface , and a magnet housing region defined by a second surface in thermal communication with the first surface , the system comprising: a cartridge platform comprising a magnet receiving slot and operable to receive the cartridge and align the second surface of the cartridge with the magnet receiving slot,', 'a heater operable to transmit heat to the heating region of the cartridge at the first surface, and', 'a magnet coupled to a magnet heating element that heats the magnet, the magnet operable to pass through the magnet receiving slot into the magnet housing region, thereby facilitating separation of a nucleic acid volume from the sample and heating the sample in cooperation with the heater., 'a molecular diagnostic module comprising2. The system of claim 1 , wherein the magnet is one of a set of magnets claim 1 , each magnet in the set of magnets coupled to ...

GENOMIC ANALYSIS DEVICE AND METHODS OF USE

Aspects of the present disclosure include a complete genomic analysis device and methods of use thereof for high throughput genomic analysis of biological samples. 1. A complete genomic analysis device , the device comprising:a thermal chip module comprising multiple self-contained composite liquid cell (CLC) locations;a CLC production station configured to access each self-contained CLC location of the thermal chip module;a sample receiving location;a reagent receiving location;a robotically controlled liquid handler configured to transfer liquid between the sample receiving location, the reagent receiving location, and the thermal chip module; andan interrogation station configured to interrogate each self-contained CLC location of the thermal chip module.2. The device according to claim 1 , wherein the thermal chip module comprises from 1400 to 3000 self-contained CLC locations.3. The device according to claim 1 , wherein the device comprises a mechanically actuated lid for the thermal chip module.4. The device according to claim 1 , wherein the reagent receiving location is configured to receive an assay plate and a master mix plate.5. The device according to claim 1 , wherein the robotically controlled liquid handler comprises interchangeable heads configured for sample dispense claim 1 , assay dispense claim 1 , and master mix dispense operations.6. The device according to claim 1 , wherein the CLC production station is configured to dispense carrier and encapsulating fluid into the self-contained CLC locations and wash the self-contained CLC locations.7. The device according to claim 1 , wherein the device comprises a fluidics module comprising liquid reservoirs for system fluids and waste collection.8. The device according to claim 1 , wherein the interrogation station is configured to detect an optical signal.9. The device according to claim 8 , wherein the interrogation station is configured to transmit excitation light to and collect emission light from ...

MULTIPLEX SLIDE PLATE DEVICE HAVING STORAGE TANK

A multiplex slide plate device is provided, including a slide plate, a sacrificial layer, and a housing. The slide plate has reaction vessels arranged in an array. The sacrificial layer has a microfluidic channel, which has an injection channel, a main channel, and a distal channel connected to each other. The housing is used to accommodate the slide plate and the sacrificial layer, and is composed of a cover and a tray, and the cover has an injection hole, an exhaust hole, and a storage tank. A sample solution and an oil are injected from the injection hole into the injection channel, wherein the sample solution is pushed by the oil. The sample solution loads into each of the reaction vessels while flowing through the main channel. Excess waste liquid flows from the exhaust hole into the storage tank, and is covered by the oil and cannot reflow. 1. A multiplex slide plate device , for a polymerase chain reaction , comprising:a slide plate having a plurality of reaction vessels, a first injection hole, and a first exhaust hole, wherein the reaction vessels are arranged in an array, and each of the reaction vessels has an opening portion and a bottom portion;a sacrificial layer having a microfluidic channel, wherein the microfluidic channel has an injection channel, a main channel, and a distal channel connected to each other, the sacrificial layer is assembled to the slide plate, and the main channel is assembled facing the opening portion; anda housing to accommodate the slide plate and the sacrificial layer and composed of a cover and a tray, wherein the cover is assembled to the tray, and the cover has a second injection hole, a second exhaust hole, and a storage tank,wherein a sample solution and an oil are sequentially injected from the second injection hole and the first injection hole into the injection channel, and the sample solution is pushed by the oil, such that the sample solution and the oil flow from the injection channel through the main channel to ...

Systems and methods for biological analysis

A biological analysis system is provided. The system comprises a sample block assembly. The sample block assembly comprises a sample block configured to accommodate a sample holder, the sample holder configured to receive a plurality of samples. The system also comprises a control system configured to cycle the plurality of samples through a series of temperatures. The system further comprises an automated tray comprising a slide assembly, the tray configured to reversibly slide the sample block assembly from a closed to an open position to allow user access to the plurality of sample holders.

Lab on chip cartridge

A cartridge for an optical analysis, including a supporting body, having a first face and a second face opposite to one another; a plurality of wells adapted to receive a biological solution to be analyzed, the wells extending in the supporting body on the first face; at least one biocompatible layer extending inside each well; an anti-reflection layer extending on the first face outside the wells; and a reflection layer extending inside each well. Methods of using same are also provided.

Thermo-controllable high-density chips for multiplex analyses

The present invention provides miniaturized instruments for conducting chemical reactions where control of the reaction temperature is desired or required. Specifically, this invention provides chips and optical systems for performing and monitoring temperature-dependent chemical reactions. The apparatus and methods embodied in the present invention are particularly useful for high-throughput and low-cost amplification of nucleic acids. 120.-. (canceled)21. A method for dispensing reaction samples into micro wells in a humidified chamber at a temperature at or near the dew point comprising: (a) a chip for cycling at least two temperature levels, said chip comprising an array of fluidically isolated micro wells, wherein each of said micro wells is un-filled and configured for receiving a reaction sample, wherein the micro wells are coated with a hydrophobic coating suitable for the nucleic acid amplification reaction, wherein each of the micro wells has an open top surface and a closed bottom surface, wherein each micro well has a volume of approximately 0.001 μl to 10 μl, and wherein the chip is configured to be in thermal contact with a heating element;', '(b) a chamber containing ambient air; and', '(c) a dispenser located inside said chamber and configured to dispense the plurality of reaction samples separately into the fluidically isolated micro wells; and, 'i) providing a system comprising (a) said ambient air in said chamber becomes saturated with moisture with respect to the planar surface of said chip thereby causing said chamber to become a humidified chamber, wherein the temperature inside said humidified chamber is at, or 1-5 degrees Celsius above, the dew point; and', '(b) said dispenser dispenses said plurality of reaction samples separately into said unfilled fluidically isolated microwells inside said humidified chamber., 'ii) placing said chip in said chamber and activating said system such that22. The method of wherein the chip further comprises ...

HONEYCOMB TUBE

A honeycomb tube with a planar frame defining a fluidic path between a first planar surface and a second planar surface. A fluidic interface is located at one end of the planar frame. The fluidic interface has a fluidic inlet and fluidic outlet. The fluidic path further includes a well chamber having an well-substrate with a plurality of wells. The well chamber is arranged in the planar frame between the first or second surface and the well-substrate. 129.-. (canceled)30. A reaction tube comprising:a body comprising a fluidic interface disposed at one end of body, wherein the fluidic interface comprises a fluidic inlet and a fluidic outlet, wherein the body defines a planar fluidic path fluidically connecting the fluidic inlet to the fluidic outlet, and wherein the fluidic path comprises an inlet passage, an outlet passage and a well chamber; anda planar well-substrate defining a plurality of wells positioned within the well chamber,wherein the inlet passage is fluidically coupled between the fluidic inlet and the well chamber and the outlet passage is fluidically coupled between the fluidic outlet and the well chamber, andwherein the inlet passage and the outlet passage are located between the well chamber and the fluidic interface.31. The reaction tube of claim 30 , wherein the body is configured to be vertically oriented so that the planar fluidic path and planar well-substrate are vertically oriented with the fluidic outlet positioned above the fluidic inlet claim 30 , and the outlet passage positioned above the inlet passage.32. The reaction tube of claim 30 , wherein the fluidic path further comprises an intermediate channel fluidically coupled between the inlet passage and the well chamber claim 30 , and wherein the intermediate channel includes a plurality of channel portions connected in a serpentine manner.33. The reaction tube of claim 32 , wherein the body is configured to be vertically oriented so that the planar fluidic path and planar well-substrate ...

COMPOSITION FOR PREVENTING EVAPORATION OF REACTION SOLUTION DURING NUCLEIC ACID AMPLIFICATION REACTION.

The present invention provides a composition capable of hermetically sealing a PCR reaction vessel without using a closure member or adhesive seal. Disclosed is a composition for preventing evaporation of a nucleic acid amplification reaction solution during nucleic acid amplification reaction, which is a liquid during the reaction and becomes a solid through chemical or thermal changes after completion of the reaction. Also disclosed is a composition for preventing evaporation of a nucleic acid amplification reaction solution during nucleic acid amplification reaction, wherein the melting point of the composition is 0-15° C. Also disclosed is a composition for preventing evaporation of a nucleic acid amplification reaction solution during nucleic acid amplification reaction, wherein the melting point of the composition is 5-10° C. 1. A composition for preventing evaporation of a nucleic acid amplification reaction solution during nucleic acid amplification reaction , which is a liquid during the reaction and becomes a solid through chemical or thermal changes after completion of the reaction.2. A composition for preventing evaporation of a nucleic acid amplification reaction solution during nucleic acid amplification reaction , wherein the melting point of the composition is 0-15° C.3. A composition for preventing evaporation of a nucleic acid amplification reaction solution during nucleic acid amplification reaction , wherein the melting point of the composition is 5-10° C.4. A method of nucleic acid amplification , comprising a step of overlayering the composition according to any one of to on top of the nucleic acid amplification reaction solution.5. A method of nucleic acid amplification , comprising a step of solidifying the composition according to any one of to after completion of the nucleic acid amplification reaction.6. A method of nucleic acid amplification , which uses a combination of a composition for preventing evaporation of a nucleic acid ...

SYSTEMS AND METHODS FOR POOLING SAMPLES FROM MULTI-WELL DEVICES

Provided herein are systems and methods for pooling samples from separated sub-arrays in multi-well devices into collection wells of a multi-well sample collection device (e.g., allowing samples in a 100 well sub-array in a 9600-well chip to be pooled into a single collection well of a 96-well plate). In certain embodiments, the systems are composed of: i) a multi-well device, ii) an extraction device; and iii) an extraction device gasket. Also provided herein are dual barcoding (e.g., X-Y barcoding), pooling (e.g., dual pooling), RNA amplification methods (e.g., for single cell analysis), that may employ the extraction devices described herein. 1. A system comprising: i) a first multi-well device comprising a plurality of separated sub-arrays, wherein each separated sub-array comprises a plurality of individual sample wells, or', 'ii) a multi-well through-hole device comprising a plurality of holes, wherein said multi-well through-hole device, when combined with a backing, forms a second multi-well device which comprises a plurality of separated sub-arrays, wherein each separated sub-array comprises a plurality of individual sample wells;, 'a) a sample device, wherein said sample device is eitherb) an extraction device comprising a plurality of fluid conduit openings and a plurality of fluid conduits, wherein each of said fluid conduit openings is attached to, or integral with, one of said fluid conduits; andc) an extraction device gasket having a top surface and a bottom surface, wherein said extraction device comprises a plurality of gasket openings that match one-for-one and align with both said plurality of separated sub-arrays in said sample device and said plurality of conduit openings in said extraction device, and i) said top surface is in contact with, and aligns with, said sample device, and', 'ii) said bottom surface is in contact with, and aligns with, said extraction device., 'wherein said extraction gasket forms a seal between said extraction device ...

Thermo-Controllable High-Density Chips for Multiplex Analyses

The present invention provides miniaturized instruments for conducting chemical reactions where control of the reaction temperature is desired or required. Specifically, this invention provides chips and optical systems for performing and monitoring temperature-dependent chemical reactions. The apparatus and methods embodied in the present invention are particularly useful for high-throughput and low-cost amplification of nucleic acids. 120-. (canceled)21. A method comprising:a. dispensing a plurality of samples into fluidically isolated micro wells of an array of 1000 or more fluidically isolated microwells; andb. detecting a chemical reaction in one or more of said fluidically isolated micro wells.22. The method of claim 21 , wherein said chemical reaction comprises a change in chemical properties of a substance.23. The method of claim 22 , wherein said substance is selected from the group consisting of: proteins claim 22 , glycoproteins claim 22 , nucleic acids claim 22 , lipids claim 22 , and inorganic chemicals claim 22 , or any combination thereof.24. The method of claim 21 , wherein said chemical reaction comprises an enzymatic reaction.25. The method of claim 21 , wherein said chemical reaction comprises an interaction selected from the group consisting of: interaction between nucleic acid molecules claim 21 , interaction between proteins claim 21 , interaction between nucleic acid and protein claim 21 , and interaction between protein and small molecules claim 21 , or any combination thereof.26. The method of claim 21 , wherein said chemical reaction is selected from the group consisting of: a redox reaction claim 21 , a hydrolysis reaction claim 21 , a phosphorylation reaction claim 21 , and a polymerization reaction claim 21 , or any combination thereof.27. The method of claim 21 , wherein said chemical reaction comprises a protein-protein interaction.28. The method of claim 27 , wherein a protein in said protein-protein interaction is an antigen or ...

Thermo-Controllable High-Density Chips for Multiplex Analyses

The present invention provides miniaturized instruments for conducting chemical reactions where control of the reaction temperature is desired or required. Specifically, this invention provides chips and optical systems for performing and monitoring temperature-dependent chemical reactions. The apparatus and methods embodied in the present invention are particularly useful for high-throughput and low-cost amplification of nucleic acids. 120-. (canceled)21. A composition comprising: a chip comprising at least 1000 fluidically isolated microwells , wherein said fluidically isolated microwells are at a surface density of 10 or more microwells per cm.22. The composition of claim 21 , wherein said fluidically isolated microwells are at a surface density of 100 or more microwells per cm.23. The composition of claim 21 , wherein said fluidically isolated microwells are at a surface density of from 5-50 microwells per mm.24. The composition of claim 21 , wherein said fluidically isolated microwells are at a surface density of at 1 or more microwell per mm.25. The composition of claim 21 , wherein said chip has a length from a few millimeters to 50 centimeters.26. The composition of claim 21 , wherein said chip has a width from a few millimeters to 50 centimeters.27. The composition of claim 21 , wherein said fluidically isolated microwells have a volume of 1 μL or less.28. The composition of claim 21 , wherein said fluidically isolated microwells have a volume ranging from 0.001 μL to 100 μL.29. The composition of claim 21 , wherein said chip comprises a material selected from the group consisting of: silicon claim 21 , silicates claim 21 , gallium phosphide claim 21 , glass claim 21 , ceramic claim 21 , metals claim 21 , and alloys claim 21 , or any combination thereof.30. The composition of claim 21 , wherein said chip comprises aluminum alloy.31. The composition of claim 21 , wherein said chip has a thermal conductivity value of 1 W/mK or greater.32. The composition of ...

Freezing stem apparatus and method

A robotic microfluidic incubator system has a thin transparent sidewall and close proximity of the embryo/oocyte/cultured cells to the sidewall allow close approach of a side view microscope with adequate focal length for mid to high power. This arrangement permits microscopic examination of multiple culture wells when arranged in rows (linear or along the circumference of a carousel). Manual or automated side to side movement of the linear well row, or rotation of the carousel, allows rapid inspection of the contents each well. Automated systems with video capability also allow remote inspection of wells by video connection or Internet connection, and automated video systems can record oft-hours inspections or time lapse development in culture (i.e. embryo cell division progression, or axon growth in neuron cell cultures). 1. A freezing stem comprising:a microfluidic chip;the chip having at least one port;an extension from the chip;the extension having a smaller width than the chip; andat least one microchannel extending between the chip and the extension.2. The freezing stem of wherein the chip has two ports.3. The freezing stem of wherein two microchannels extend between the chip and the extension.4. The freezing stem of wherein one microchannel is larger than the other.5. The freezing stem of wherein the chip is transparent.6. The freezing stem of wherein the chip is opaque.7. The freezing stem of wherein the extension is transparent.8. The freezing stem of wherein the extension is opaque.9. The freezing stem of further comprising a cap to cover the extension.10. The freezing stem of wherein the chip has multiple extensions.11. The freezing stem of wherein more than one chip is removably attached to a parallel media flow system.12. The freezing stem of wherein the chip has a body claim 1 , the body having a base claim 1 , and an extension is located on the base of the chip body.13. The freezing stem of wherein the chip has a body claim 1 , the body having a side ...

Micromanipulation apparatus and method

A robotic microfluidic incubator system has a thin transparent sidewall and close proximity of the embryo/oocyte/cultured cells to the sidewall allow close approach of a side view microscope with adequate focal length for mid to high power. This arrangement permits microscopic examination of multiple culture wells when arranged in rows (linear or along the circumference of a carousel). Manual or automated side to side movement of the linear well row, or rotation of the carousel, allows rapid inspection of the contents each well. Automated systems with video capability also allow remote inspection of wells by video connection or Internet connection, and automated video systems can record oft-hours inspections or time lapse development in culture (i.e. embryo cell division progression, or axon growth in neuron cell cultures). 1. A method for use with a specimen , and for use in an environment having gravity defining upward and downward directions , and for use relative to a horizontal surface having a suction port microchannel located below the horizontal surface , the suction port microchannel being too narrow to permit passage of the specimen therethrough; the method comprising the steps of:providing a liquid medium above the horizontal surface;providing a specimen within the liquid medium;holding the specimen on the horizontal surface by means of suction at the suction port microchannel;providing a micromanipulation tool manipulated by a microactuator, the microactuator in air and not within the liquid medium;moving the micromanipulation tool downwards through the air and through the surface of the liquid medium to approach and contact the top of the specimen.2. The method of wherein the specimen is an oocyte.3. The method of wherein the specimen is an embryo.4. The method of wherein the specimen is from a human organ.5. The method of wherein the specimen is from a human organ.6. Apparatus for use with a specimen claim 3 , the apparatus for use in an environment ...

Microfluidic Embryo and Gamete Culture Systems

A robotic microfluidic incubator system has a thin transparent sidewall and close proximity of the embryo/oocyte/cultured cells to the sidewall allow close approach of a side view microscope with adequate focal length for mid to high power. This arrangement permits microscopic examination of multiple culture wells when arranged in rows (linear or along the circumference of a carousel). Manual or automated side to side movement of the linear well row, or rotation of the carousel, allows rapid inspection of the contents each well. Automated systems with video capability also allow remote inspection of wells by video connection or Internet connection, and automated video systems can record oft-hours inspections or time lapse development in culture (i.e. embryo cell division progression, or axon growth in neuron cell cultures). 1. A microfluidic sperm separation network comprising:a sperm solution entry port;a sperm solution exit port;a media entry port;at least one network feed channel;a series of connected microchannels; andmultiple product exit ports.2. The sperm separation of further comprising at least one gradient solution entry port.3. The sperm separation network of wherein a single gradient entry port and single media entry port feed into a large chamber which terminates in parallel microchannels.4. The sperm separation network of having multiple gradient solution entry ports.5. The sperm separation network of having automated mixers in the microchannels.6. The sperm separation network of wherein the gradient solution comprises an albumin solution.7. The sperm separation network of wherein the gradient solution comprises chemotactic agents.8. The sperm separation network of wherein the gradient solution comprises pH gradients.9. The sperm separation network of wherein the gradient solution comprises a sugar gradient.10. The sperm separation network of wherein the gradient solution comprises a carbohydrate gradient.11. The sperm separation network of wherein the ...

Device for pathogen detection

There is provided a portable device for detecting pathogens in a sample, the detection being based on an assay involving one or more reagents and heat. The device comprises a flexible substrate having a plurality of spaced apart reservoirs along its length, each reservoir adapted for receiving reagents and the sample, the substrate being secured to a pair of spaced apart reels and movable there between by rolling. Also, the device comprises heating means which can be secured to one of the two reels.

THERMAL CYCLER HAVING A TEMPERATURE ANALYSIS AND/OR VERIFICATION UNIT AND A METHOD FOR ANALYZING OR VERIFYING A THERMAL PERFORMANCE OF A THERMAL CYCLER AND FOR CALIBRATING THE THERMAL CYCLER

The invention relates to a thermal cycler () comprising a housing (), the housing () accommodating a thermal block () having a plurality of sample wells (), each for receiving a test sample in a sample vessel, electric heater means () for heating the thermal block (), a power supply () and an electronic control () for controlling the electric heater means (), and further comprising a temperature analysis and/or verification unit () for analyzing and/or verifying a thermal performance of the thermal block (). The invention further relates to a method for analyzing or verifying a thermal performance of a thermal cycler () and for calibrating the thermal cycler (). The thermal cycler () is characterized in that the temperature analysis and/or verification unit () is integrated into the housing () and is connected to the power supply () and to the electronic control () by means of an internal interface (), whereas the method is characterized by the following steps: providing the thermal cycler () with an integrated temperature analysis and/or verification unit () and using the integrated temperature analysis and/or verification unit () for self-calibration of the thermal cycler (). 228183632. The thermal cycler according to claim 1 , wherein the temperature analysis and verification unit () comprises a closed loop control for calibration of the electric heater means () in dependency of the temperature measured by the temperature probes () within selected sample wells ().33452145214. The thermal cycler according to claim 1 , wherein the temperature probe plate () is accommodated within a stowage compartment () of the housing () and is removable from the stowage compartment () for placement on the thermal block ().4345236423452345452. The thermal cycler according to claim 1 , wherein the temperature probe plate () is accommodated within a stowage compartment () claim 1 , and wherein the temperature probes () project from a broad side () of the temperature probe plate () ...

MICROPLATE HAVING REGIONS OF DIFFERENT THICKNESSES

A method of forming microplate includes forming a preform microplate including a deck and a plurality of wells, and reforming at least one well of the plurality of wells to provide a microplate. Each well of the plurality of wells in the preform microplate has a first depth and a first wall thickness. At least one well of the microplate has a second depth and a second wall thickness, where the second depth is greater than the first depth and/or the second wall thickness is less than the first wall thickness. 1. A method of forming a microplate , comprising:providing a preform microplate comprising a deck and a plurality of wells, each well of the plurality of wells having a first depth and a first wall thickness, andreforming at least one well of the plurality of wells to provide a microplate comprising at least one well having a second depth and a second wall thickness,wherein at least one of the second depth is greater than the first depth, or the second wall thickness is less than the first wall thickness.2. The method according to claim 1 , wherein the second depth is greater than the first depth claim 1 , and the second wall thickness is less than the first wall thickness.3. The method according to claim 1 , wherein providing a preform microplate comprises forming a preform microplate.4. The method according to claim 3 , wherein forming a preform microplate comprises a process chosen from injection molding claim 3 , drawing by at least one of a plurality of core pins and a cavity block claim 3 , and combinations thereof.5. The method according to claim 1 , wherein the reforming comprises drawing by at least one of a plurality of core pins and a cavity block.6. The method according to claim 5 , wherein the cavity block comprises at least one vent.7. The method according to claim 5 , wherein the drawing comprises forming a vacuum.8. The method according to claim 5 , wherein at least one of the plurality of core pins and the cavity block is heated.9. The method ...

MICROPLATE AND METHODS FOR MAKING THE SAME

A microplate () includes a carrier () having a plate () and an annular perimeter wall () to define a recess (). An array of holes () extends through the plate (). A tape piece (), die cut from a flexible tape () includes an array of wells () each extending through and having an opening () extending into the well (). The array of wells () has a number and locations corresponding to the array of holes (). The openings () have sizes corresponding to the holes (). An upper surface () of the tape piece () is abutted with and bonded to the bottom face () of the plate () with the openings () corresponding to the array of holes (). The slideable receipt of an annular outer periphery () of the tape piece () insures that the array of wells () are aligned to correspond to the array of holes () as die cutting of tape piece () insures that the array of wells () are at consistent positions relative to the annular outer periphery (). 1. A microplate comprising , in combination:a carrier including a plate having a top face, a bottom face spaced from the top face in a direction, and an annular perimeter, with the plate including an array of holes extending from the top face and located inside the annular perimeter, with the bottom face being planar; anda tape piece made of a single piece, with the tape piece including an upper surface and a lower surface spaced a thickness from the upper surface in the direction, with the tape piece further including an array of wells each integrally extending in the direction away from the upper and lower surfaces and each having an opening extending from the upper surface into the well and an integral bottom spaced from the opening, with the array of wells having a number and locations substantially corresponding to the array of holes of the plate, with the openings of the array of wells having sizes substantially corresponding to the array of holes of the plate, with the upper surface coupled to one of the faces of the plate with the openings of ...

Reinforced microplate

A microplate is fitted with a reinforcing member that enhances the rigidity of the microplate and decreases the magnitude of thermally-induced deformation. An example reinforced microplate includes a deck including a plurality of wells formed therein, and a reinforcing member attached to an underside of the deck.

CAPS FOR SAMPLE WELLS AND MICROCARDS FOR BIOLOGICAL MATERIALS

A cover for a biological sample well tray, comprising a cap for sealing a sample well. The cap comprises a well lens for focusing light into the sample well and collecting light from the sample. In another aspect, the cap comprises an elongate portion configured to permit incoming light to pass into the sample well and out of the sample well. Various other aspects comprise a microcard for biological material, and an apparatus for a plurality of sample well strips. A method for testing a biological sample is also provided. 1. A device for holding samples of biological material , comprising:a plurality of sample wells, wherein each sample well comprises a well lens for focusing light into the sample and collecting light emitted from the sample, wherein the well lens provides a bottom for the sample well.2. The apparatus of claim 1 , wherein the plurality of sample wells is adapted for use in a thermal cycling device.3. The apparatus of claim 2 , wherein the thermal cycling device performs real-time detection.4. The apparatus of claim 2 , wherein the thermal cycling device performs endpoint detection.5. The system of claim 1 , wherein the well lens is plano-convex.6. The system of claim 1 , wherein the well lens is a Fresnel lens.7. The system of claim 1 , wherein the well lens is aplanatic.8. The apparatus of claim 1 , wherein the plurality of wells are provided in a sample well tray.9. The apparatus of claim 9 , wherein the sample well tray is a microcard.10. A system for analyzing samples of biological material claim 9 , comprising:a plurality of sample wells, wherein each sample well comprises a well lens for focusing light into the sample and collecting light emitted from the sample, and wherein the well lens provides a bottom for the sample well;a light source for providing excitation light to the sample wells; anda detector, wherein said detector provides a surface area to detect the collected light from each sample well, wherein the surface area comprises ...

WIDE-ANGLE EMISSION FILTER, OPTICAL SENSOR ASSEMBLY HAVING THE SAME, PCR SYSTEM HAVING THE SAME, AND METHOD OF MANUFACTURING THE SAME

The wide-angle emission filter includes a base matrix, a photoresist, and a colorant. The base matrix has a flat shape and including a transparent material. The base matrix does not generate fluorescent light or phosphorescent light by an excitation light. The photoresist is disposed in the base matrix. The photoresist is fixed in a solid state through at least one method selected from the group consisting of thermal hardening, photo hardening, and drying. The colorant is disposed in the base matrix and includes light having a predetermined wavelength range. The wide-angle emission filter filters the excitation light regardless of an incident angle of the excitation light. 1. A wide-angle emission filter comprising:a base matrix having a flat shape and including a transparent material, the base matrix not generating fluorescent light or phosphorescent light by an excitation light;a photoresist disposed in the base matrix, the photoresist being fixed in a solid state through at least one method selected from the group consisting of thermal hardening, photo hardening, and drying; anda colorant disposed in the base matrix and including light having a predetermined wavelength range,wherein the wide-angle emission filter filters the excitation light regardless of an incident angle of the excitation light.2. The wide-angle emission filter of claim 1 , wherein the wide-angle emission filter is disposable and used for one time test.3. The wide-angle emission filter of claim 1 , wherein the photoresist comprises a semi-solidified photoresist claim 1 , which is not completely saturated by light having a short wavelength claim 1 , and the excitation light is firstly filtered by the colorant and is secondly filtered by the semi-solidified photoresist.4. The wide-angle emission filter of claim 3 , wherein the photoresist further comprises a saturated photoresist disposed in the base matrix claim 3 , which is completely saturated by the excitation light.5. An optical sensor ...

MOBILE DEVICE FOR THE ISOLATION OF NUCLEIC ACIDS

The invention relates to a mobile device system, comprising a hand-held device and a test kit for the mobile isolation of nucleic acids. The hand-held device comprises at least one sample block for inserting sample containers, a sample block holder with boreholes or recesses for accommodating the sample blocks, a device base with electronic control units for the sample blocks, a voltage source and a connection to the sample block holder, as well as a test kit for the isolation of nucleic acids. The hand-held device is characterized in that the sample blocks can be removed from the sample block holder and the sample block holder can be removed from the device base. 120-. (canceled)21: A method for mobile isolation of a nucleic acid , comprising: a device base having at least one electronic control unit;', 'a sample block holder having at least one bore configured to receive and hold at least one removable sample block, the sample block holder being in electrical communication with and detachably fixed to the device base; wherein the sample block holder is detachably fixed to the device base by at least one plug connector or by a magnetic attraction and wherein the sample block holder comprises a variable shaking device; and', 'the sample reaction vessel, which is configured to insert into an open cavity of the at least one removable sample block;', 'a voltage supply; and', at least one component selected from the group consisting of:', 'a) a Pasteur pipette or other disposable pipette;', 'b) a bottle for reagent waste:', 'c) a suspension containing magnetic or paramagnetic particles;', 'd) a lysis buffer;', 'e) a proteolytic enzyme in liquid form;', 'f) a binding buffer:', 'g) a detergent buffer; and', 'h) an elution buffer;, 'a test kit for mobile detection of the nucleic acid, comprising, 'wherein the at least one removable sample block is configured to insert into the at least one bore of the sample block holder, and to electrically communicate with the at least ...

Sample Holder and Lock-In Thermography System With Such

A sample holder for magnetic nanoparticle samples including a plurality of sample wells for holding a magnetic nanoparticle sample, which are distributed on a top surface of the sample holder. The sample wells are distributed such that a first distance between neighboring samples wells and/or a second distance between each peripheral sample well and a respective edge of the top surface of the sample holder and/or a third distance between a deepest point of the sample wells and a bottom of the sample holder is between 1 and 100 times greater than a thermal diffusion length of the sample holder material. The sample holder is used in a lock-in thermography system. 2. The sample holder according to claim 1 , wherein a width of the sample wells is 1 to 100 times greater than a thermal diffusion length of the magnetic nanoparticle sample.3. The sample holder according to claim 1 , wherein the first distance and the second distance and the third distance are equal.4. The sample holder according to claim 1 , wherein the material of the sample holder is plastic or polymer claim 1 , chosen from a group consisting of polyethylene HD claim 1 , polyethylene SD claim 1 , polystyrene claim 1 , and polypropylene.5. The sample holder according to claim 1 , wherein the sample holder consists of a sample holder material which is opaque to infrared thermal radiation.6. The sample holder according to claim 1 , wherein the top surface of the sample holder has an infrared emissivity between 0.1 and 1.8. The lock-in thermography system according to claim 7 , wherein the thermal imaging device comprises either an infrared camera with an optical lens focused on the top surface of the sample holder or comprises an array of infrared sensors with a corresponding array of lenses claim 7 , particularly plastic lenses claim 7 , wherein a focus of the lenses is adjustable such that the entire top surface of the sample holder is focused on the thermal imaging device.9. The lock-in thermography ...

VACUUM ASSIST FOR A MICROPLATE

A vacuum assist apparatus can comprise a microplate. The microplate can comprise a first surface and an opposing second surface. A plurality of wells can be formed in the first surface of the microplate. Each of the plurality of wells can be sized to receive an assay therein. A support base can comprise a fluid passage. The microplate can be positioned adjacent and in contact with the support base. A pressure device, in fluid communication with the fluid passage, can exert a vacuum within the fluid passage to actively retain the microplate in the contact with the support base. 1. An apparatus to aid in genomic analysis , the apparatus comprising:a substrate comprising one or more material retention regions, for retaining a material;a marking indicia located on or within the substrate; anda reader for reading the marking indicia.2. The apparatus of claim 1 , wherein the marking indicia is an RFID tag and the reader station is an RFID reader.3. The apparatus of claim 2 , wherein the RFID reader automatically reads the RFID tag to provide a unique identification and/or data handling parameters of the substrate.4. The apparatus of claim 1 , wherein the marking indicia is configured to identify the substrate with a unique identification.5. The apparatus of claim 4 , wherein the substrate identification correlates acquired data with a corresponding assay.6. The apparatus of claim 1 , wherein the marking indicia can assist in achieving a predetermined position of the substrate.7. The apparatus of claim 1 , wherein the marking indicia can be selected so as to reduce thermal and/or chemical interference.8. A thermal system claim 1 , the system comprising:at least one sample block comprising a first surface and a second surface, wherein the first surface is capable of supporting a substrate;at least one channel in the first surface of the sample block, wherein the at least one channel is located between the sample block and the substrate;a vacuum source fluidly coupled to the ...

THREE-STAGE THERMAL CONVECTION APPARATUS AND USES THEREOF

Disclosed is a multi-stage thermal convection apparatus and uses thereof. In one embodiment, the invention features a three-stage thermal convection apparatus that includes a temperature shaping element for assisting a thermal convection mediated Polymerase Chain Reaction (PCR). The invention has a wide variety of applications including amplifying nucleic acid without cumbersome and expensive hardware associated with many prior devices. In a typical embodiment, the apparatus can fit in the palm of a user's hand for use as a portable, simple to operate, and low cost PCR amplification device. 1. An apparatus adapted to perform thermal convection PCR comprising:(a) a first heat source for heating or cooling a channel and comprising a top surface and a bottom surface, the channel being adapted to receive a reaction vessel for performing PCR,(b) a second heat source for heating or cooling the channel and comprising a top surface and a bottom surface, the bottom surface facing the top surface of the first heat source,(c) a third heat source for heating or cooling the channel and comprising a top surface and a bottom surface, the bottom surface facing the top surface of the second heat source, wherein the channel is defined by a bottom end contacting the first heat source and a through hole contiguous with the top surface of the third heat source, and further wherein center points between the bottom end and the through hole form a channel axis about which the channel is disposed,(d) at least one temperature shaping element such as at least one protrusion in at least one of the first, second and third heat sources, the protrusion being disposed about the channel axis and extending toward an adjacent heat source or away from the top or bottom surface of the heat source that comprises the protrusion; and(e) a receptor hole adapted to receive the channel within the first heat source.2. The apparatus of claim 1 , wherein the apparatus comprises a first insulator positioned ...

INTEGRATED SYSTEM FOR NUCLEIC ACID AMPLIFICATION AND DETECTION

Devices, systems, methods, and kits are disclosed for amplifying small quantities of nucleic acid and identifying the nucleic acid sequences of the amplified nucleic acid products. 1. A device for collection of a biological sample comprising nucleic acid from an individual , comprising a base substrate component having at least a first surface area , wherein the at least first surface area is:a. transparent or translucent to an energy wave capable of imaging at least one ridge and valley signature of an appendage of the individual;b. configured to collect the biological sample from the appendage; andc. comprised of one or more materials compatible with reaction conditions of a nucleic acid amplification reaction.2. The device of claim 1 , wherein the base substrate component is polymeric film or glass.3. The device of claim 2 , wherein all surface areas of the substrate component are transparent or translucent to the energy wave.4. The device of claim 2 , wherein all surface areas of the base substrate component are configured to be compatible with the nucleic acid amplification reaction conditions.5. The device of any of the preceding claims claim 2 , wherein the at least first surface area is hydrophilic.6. The device claim 2 , wherein the base substrate component further comprises a second surface area surrounding the at least first surface area wherein the second surface area is hydrophobic.7. The device of claim 2 , wherein the device further comprises a frame component to support the substrate component.8. The device of claim 7 , wherein the frame component is formed from a material selected from a polymer claim 7 , a metal claim 7 , a metal alloy claim 7 , a glass claim 7 , a silicon material or combinations thereof.9. The device of claim 2 , further comprising a mask component claim 2 , wherein the mask component is configured to:d. attach to the base substrate component;e. surround the at least first surface area of the base substrate component; andf. ...

OPERATION METHOD OF MULTIPLEX SLIDE PLATE DEVICE

An operation method of a multiplex slide plate device is provided. First, the multiplex slide plate device is assembled, including a slide plate, a sacrificial layer and a housing. The slide plate has reaction vessels, and the sacrificial layer has a microfluidic channel composed of an injection channel, a main channel and a distal channel. A sample solution is injected to the injection channel, such that the sample solution flows from the injection channel through the main channel to the distal channel, wherein the sample solution loads into the reaction vessels. Afterwards, an oil is injected to the injection channel, such that the oil flows from the injection channel through the main channel to the distal channel, wherein the oil removes the sample solution not loaded into the reaction vessels. Next, the sacrificial layer is heated to melt, and the melted sacrificial layer is mixed with the oil. 1. An operation method of a multiplex slide plate device , including:assembling the multiplex slide plate device including a slide plate, a sacrificial layer and a housing for accommodating the slide plate and the sacrificial layer, and the slide plate has a plurality of reaction vessels arranged in an array, wherein each of the reaction vessels has an opening portion and a bottom portion, and the sacrificial layer has a microfluidic channel composed of an injection channel, a main channel and a distal channel connected to each other, wherein the main channel faces the opening portion;injecting a sample solution to the injection channel through an injection hole of the housing, such that the sample solution flows from the injection channel through the main channel to the distal channel, wherein the sample solution loads into each of the reaction vessels while flowing through the main channel;injecting an oil to the injection channel through the injection hole of the housing, such that the oil flows from the injection channel through the main channel to the distal channel, ...

SYSTEM AND METHOD FOR PROCESSING AND DETECTING NUCLEIC ACIDS

A system and method for processing and detecting nucleic acids from a set of biological samples, comprising: a capture plate and a capture plate module configured to facilitate binding of nucleic acids within the set of biological samples to magnetic beads; a molecular diagnostic module configured to receive nucleic acids bound to magnetic beads, isolate nucleic acids, and analyze nucleic acids, comprising a cartridge receiving module, a heating/cooling subsystem and a magnet configured to facilitate isolation of nucleic acids, a valve actuation subsystem configured to control fluid flow through a microfluidic cartridge for processing nucleic acids, and an optical subsystem for analysis of nucleic acids; a fluid handling system configured to deliver samples and reagents to components of the system to facilitate molecular diagnostic protocols; and an assay strip configured to combine nucleic acid samples with molecular diagnostic reagents for analysis of nucleic acids. 1. A system for processing nucleic acid content of one or more samples in cooperation with a cartridge comprising a heating region defined at a first surface , the heating region spanning a set of microfluidic pathways of the cartridge for performing amplification and detection of said nucleic acid content by way of the set of microfluidic pathways , the system comprising: a cartridge location within the molecular diagnostic module,', 'one or more heaters operable to transmit heat to the heating region of the cartridge at the first surface;, 'a molecular diagnostic module comprisingone or more storage containers for one or more reagents;a fluid handling subsystem comprising an actuator and an aspiration and delivery head coupled to the actuator; and receiving the cartridge at the cartridge location,', 'positioning the first surface of the cartridge relative to the one or more heaters,', 'executing a reagent receiving mode with movement of the aspiration and delivery head of the fluid handling system ...

Device for analysis of a target analyte

This disclosure is directed to a slide for analyzing target analytes of a suspension. The slide can be used to collect and hold the target analyte for imaging or further processing. The slide comprises a well section, including wells into which the target analyte can be stored. The wells may be removable and sized to fit into a second apparatus, such as a PCR thermocycler, for additional processing. Alternatively, the wells may hold processing vessels, such as PCR tubes, the processing vessels being used for downstream processing and/or analysis.

SAMPLE CONTAINER FOR A CRYOGENICALLY PRESERVED BIOLOGICAL SAMPLE, METHOD FOR PRODUCING THE SAMPLE CONTAINER, METHOD FOR MONITORING THE TEMPERATURE OF A CRYOGENICALLY PRESERVED SAMPLE

The invention relates to a sample container that is designed to accommodate a cryogenically preserved biological sample, and to a method for producing the sample container. The invention further relates to a method for monitoring the temperature of a cryogenically preserved biological sample. The sample container that is designed to accommodate a cryogenically preserved biological sample has, on a region of its outer surface, a frozen-solid indicator substance whose melting point is in a range from −20° C. to −140° C. 1. A sample container which is configured to receive a cryopreserved biological sample , which bears at a region of an outer surface thereof a frozen-solid indicator substance , the melting temperature of which lies in a range from −20° C. to −140° C.2. The sample container according to claim 1 , wherein a region bearing the frozen-solid indicator substance comprises a coating claim 1 , a roughening and/or a structuring.3. The sample container according to claim 1 , wherein the region bearing the frozen-solid indicator substance comprises a mirroring.4. The sample container according to claim 1 , wherein the region bearing the frozen-solid indicator substance comprises an electrode arrangement.5. The sample container according to claim 1 , wherein the indicator substance is applied in a predetermined arrangement onto the outer surface of the sample container claim 1 , wherein the predetermined arrangement represents a number claim 1 , a letter claim 1 , a symbol claim 1 , a marker and/or a structure.6. The sample container according to claim 1 , wherein the indicator substance applied on the outer surface is obtained by frozen-solid drops of the indicator substance.7. The sample container according to claim 1 , wherein several different indicator substances claim 1 , have different melting temperatures which lie in each case in a range from −20 C. to −140° C. claim 1 , are applied at different regions of the outer surface of the sample container.8. The ...

SYSTEMS AND METHODS FOR HEATING BIOLOGICAL SAMPLES

The present disclosure provides systems and methods for heating biological samples. Methods and systems of the present disclosure may be used for performing nucleic acid amplification to identify one or more targets in a nucleic acid sample of a subject. 1129.-. (canceled)130. A method for processing a biological sample comprising:(a) providing a solution comprising said biological sample and one or more heating elements in a vessel, wherein said one or more heating elements are dispersed in said solution, and wherein said one or more heating elements generate heat upon inductive coupling to a magnetic field;(b) bringing said solution in contact with said magnetic field; andsubjecting said solution comprising said biological sample to heating upon inductive coupling of said one or more heating elements to said magnetic field.131. The method of claim 130 , wherein at least a subset of said heating elements is floating claim 130 , suspended claim 130 , or dissolved in said solution.132. The method of claim 130 , wherein said solution comprises one or more droplets claim 130 , and wherein at least a subset of said one or more heating elements is in a given one of said one or more droplets.133. The method of claim 132 , wherein said one or more droplets comprise aqueous droplets in an emulsion.134. The method of claim 130 , wherein said one or more heating elements comprise particles.135. The method of claim 130 , wherein each of said one or more heating elements comprises a polymer and at least one magnetically-active material supported by said polymer claim 130 , which at least one magnetically-active material generates heat upon inductive coupling to said magnetic field.136. The method of claim 130 , wherein at least one additional heating element is used to subject said solution comprising said biological sample to heating.137. The method of claim 136 , wherein said at least one additional heating element comprises a thermoelectric material.138. The method of claim ...

INSTRUMENT FOR ANALYZING BIOLOGICAL SAMPLES AND REAGENTS

An instrument for processing a biological sample includes a chassis. Connected to the chassis is a tape path along which a tape with a matrix of wells can be automatically advanced through the instrument, a dispensing assembly for dispensing the biological sample and a reagent into the matrix of wells of the tape to form a biological sample and reagent mixture, a sealing assembly for sealing the biological sample and reagent mixture in the tape, and an amplification and detection assembly for detecting a signal from the biological sample and reagent mixture in the matrix of wells in the tape. 1. A dispensing assembly comprising:a gantry with an x-axis track and a y-axis track, the y-axis track of the gantry configured to move along the x-axis track of the gantry; a dispensing head attached to the y-axis track of the gantry below the y-axis track of the gantry, the dispensing head comprising:a contact dispensing unit; anda non-contact dispensing unit with a jet tip for dispensing a liquid;a dispensing enclosure attached to the y-axis track of the gantry on top of the y-axis track of the gantry, the dispensing enclosure having a pressure reservoir; anda tube connecting the jet tip of the non-contact dispensing unit to the pressure reservoir of the dispensing enclosure;wherein the contact dispensing unit is attached to the y-axis track of the gantry with a first z-axis track; andwherein the non-contact dispensing unit is attached to the contact dispensing unit with a second z-axis track.2. The dispensing assembly of claim 1 , wherein the dispensing head and the dispensing enclosure are configured to move simultaneously along the x-axis track and y-axis track of the gantry to minimize relative motion between the dispensing enclosure and the jet tip.3. The dispensing assembly of claim 1 , wherein the contact dispensing unit comprises a pipette tip.4. The dispensing assembly of claim 1 , wherein the contact dispensing unit comprises a plurality of pipette tips.5. The ...

System and method including thermal cycler modules

Systems and methods for processing and analyzing samples are disclosed. The system may process samples, such as biological fluids, using assay cartridges which can be processed at different processing locations. In some cases, the system can be used for PCR processing. The different processing locations may include a preparation location where samples can be prepared and an analysis location where samples can be analyzed. To assist with the preparation of samples, the system may also include a number of processing stations which may include processing lanes. During the analysis of samples, in some cases, thermal cycler modules and an appropriate optical detection system can be used to detect the presence or absence of certain nucleic acid sequences in the samples. The system can be used to accurately and rapidly process samples.