11-02-2016 дата публикации
Номер: US20160040172A1
Принадлежит:
Provided herein are non-naturally occurring microbial organisms having a FaldFP, a FAP and/or metabolic modifications which can further include a MMP, a MOP, a hydrogenase and/or a CODH. These microbial organisms can further include a butadiene, 13BDO, CrotOH, MVC or 3-buten-1-ol pathway. Additionally provided are methods of using such microbial organisms to produce butadiene, 13BDO, CrotOH, MVC or 3-buten-1-ol. 1. A non-naturally occurring microbial organism having a FaldFP and a FAP , wherein said organism comprises at least one exogenous nucleic acid encoding a FaldFP enzyme expressed in a sufficient amount to produce pyruvate , wherein said FaldFP comprises: 'wherein 1B is a 3-hexulose-6-phosphate synthase, wherein 1C is a 6P3HI, wherein 1D is a DHAS, wherein said organism comprises at least one exogenous nucleic acid encoding a FAP enzyme expressed in a sufficient amount to produce formaldehyde, pyruvate, or acetyl-CoA, wherein said FAP comprises a pathway selected from:', '(1) 1B and 1C; or (2) 1D,'} 'wherein 1E is a formate reductase, 1F is a formate ligase, a formate transferase, or a formate synthetase, wherein 1G is a formyl-CoA reductase, wherein 1H is a FTHFS, wherein 1I is a methenyltetrahydrofolate cyclohydrolase, wherein 1J is a MTHFDH, wherein 1K is a formaldehyde-forming enzyme or spontaneous, wherein 1L is a glycine cleavage system, wherein 1M is a serine hydroxymethyltransferase, wherein 1N is a serine deaminase, wherein 1O is a methylenetetrahydrofolate reductase, wherein 1P is an acetyl-CoA synthase.', '(3) 1E; (4) 1F, and 1G; (5) 1H, 1I, 1J, and 1K; (6) 1H, 1I, 1J, 1L, 1M, and 1N; (7) 1E, 1H, 1I, 1J, 1L, 1M, and 1N; (8) 1F, 1G, 1H, 1I, 1J, 1L, 1M, and 1N; (9) 1K, 1H, 1I, 1J, 1L, 1M, and 1N; and (10) 1H, 1I, 1J, 1O, and 1P,'}2. The non-naturally occurring microbial organism of claim 1 , wherein said microbial organism comprises one claim 1 , two claim 1 , three claim 1 , four claim 1 , five claim 1 , six claim 1 , seven claim 1 , or eight ...
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