17-03-2016 дата публикации
Номер: US20160076060A1
Принадлежит:
Provided herein is a non-naturally occurring microbial organism having a 1,3-butanediol (1,3-BDO) pathway and comprising at least one exogenous nucleic acid encoding a 1,3-BDO pathway enzyme expressed in a sufficient amount to produce 1,3-BDO. In some embodiments, the pathway includes reducing equivalents from CO or hydrogen. In certain embodiments, a 1,3-BDO pathway proceeds by way of central metabolites pyruvate, succinate or alpha-ketoglutarate. Also provided herein is a method for producing 1,3-BDO, includes culturing such microbial organisms under conditions and for a sufficient period of time to produce 1,3-BDO. 2. The non-naturally occurring microbial organism of claim 1 , wherein said microbial organism further comprises an exogenous nucleic acid encoding an enzyme selected from the group consisting of a pyruvate:ferredoxin oxidoreductase claim 1 , an aconitase claim 1 , an isocitrate dehydrogenase claim 1 , a succinyl-CoA synthetase claim 1 , a succinyl-CoA transferase claim 1 , a fumarase claim 1 , a malate dehydrogenase claim 1 , an acetate kinase claim 1 , a phosphotransacetylase claim 1 , an acetyl-CoA synthetase claim 1 , an NAD(P)H:ferredoxin oxidoreductase claim 1 , a ferredoxin claim 1 , and combinations thereof.3. The non-naturally occurring microbial organism of claim 1 , wherein said microbial organism further comprises an exogenous nucleic acid encoding an enzyme selected from the group consisting of a succinyl-CoA synthetase claim 1 , a succinyl-CoA transferase claim 1 , a fumarase claim 1 , a malate dehydrogenase claim 1 , and combinations thereof.4. The non-naturally occurring microbial organism of claim 1 , wherein said microbial organism comprises two claim 1 , three claim 1 , four claim 1 , five claim 1 , six claim 1 , seven claim 1 , eight or nine exogenous nucleic acids claim 1 , each encoding a 1 claim 1 ,3-BDO pathway enzyme.5. The non-naturally occurring microbial organism of claim 1 , wherein said microbial organism comprises ...
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