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Небесная энциклопедия

Космические корабли и станции, автоматические КА и методы их проектирования, бортовые комплексы управления, системы и средства жизнеобеспечения, особенности технологии производства ракетно-космических систем

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Мониторинг СМИ

Мониторинг СМИ и социальных сетей. Сканирование интернета, новостных сайтов, специализированных контентных площадок на базе мессенджеров. Гибкие настройки фильтров и первоначальных источников.

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Применить Всего найдено 10903. Отображено 100.

26-07-2012 дата публикации

Viscoelastic gels as novel fillers

Номер: US20120190644A1

Автор: Davide RENIER, Matteo D'este

Принадлежит: Fidia Farmaceutici SpA

Biomaterials obtainable by mixing the autocrosslinked derivative of hyaluronic acid (ACP) with the derivative (HBC) of hyaluronic acid crosslinked with 1,4-butanediol diglycidyl ether (BDDE) in the weight ratio of between 10:90 and 90:10 as novel fillers.

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Номер записи: 1

26-07-2012 дата публикации

Methods for Steam Flash Extraction of Pectin

Номер: US20120190831A1

Автор: Brian Rudolph, Sune Allan Petersen

Принадлежит: CP KELCO APS

Methods are provided for high temperature and short time extraction of pectins from pectin-containing plant materials. Generally described, the method includes mixing the pectin-containing plant material and an acidic aqueous medium to form a mixture; heating the mixture (optionally under pressure) to a target temperature by steam injection; maintaining the mixture under pressure at the target temperature for a time up to about 5 minutes; and flashing the mixture into a flash tank at a pressure from about 0.5 to about 1.2 bar to extract pectin from the pectin-containing plant material.

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Номер записи: 2

06-12-2012 дата публикации

Microprocessing for preparing a polycondensate

Номер: US20120309956A1

Автор: Bruno Frederic Stengel, Christof Franz Kuesters, J. Brandner, W. Benzinger

Принадлежит: Cargill Inc

The present invention relates to a process for preparing polydextrose by using a microdevice. It further relates to the use of a microdevice for the polycondesation reactions.

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Номер записи: 3

14-03-2013 дата публикации

NOVEL PROCESS

Номер: US20130066064A1

Автор: CHARLES Philippe, GELDHOF Geoffroy, Mancuso Vincent

Принадлежит:

A single-phase LPS extraction composition comprising water, an alcohol and a further organic solvent, where the amount of water is between about 0.8 to 1.2% (v/v). 1. An LPS extraction composition comprising water , an alcohol and a further organic solvent.2. The composition of wherein the LPS extraction composition is single-phase.3. The composition of or wherein the amount of water in the LPS extraction composition is between about 0.1 and about 1.5% (v/v).4. The composition of any of to wherein the amount of water is about 1% (v/v).5. The composition of any of to wherein the amount of water is about 0.5% (v/v).6. The composition of any of to wherein the alcohol is selected from the list: methanol claim 1 , ethanol claim 1 , isopropanol or butanol.7. The composition of any of to wherein the percentage of alcohol in the LPS extraction composition is between about 5% and about 40% (v/v).8. The composition of wherein the percentage of alcohol is between about 10% (v/v) and about 30% (v/v).9. The composition of any preceding claim wherein the further organic solvent is selected from the group: chloroform claim 7 , alkanes claim 7 , toluene and petroleum ether.10. The composition of wherein the alkane is selected from the group: isooctane claim 9 , ethane claim 9 , heptane and hexane.11. The composition of any preceding claim wherein the percentage of the further organic solvent in the LPS extraction composition is between about 60% (v/v) and about 95% (v/v).12. The composition of wherein the percentage of the further organic solvent is between about 75% (v/v) and about 90% (v/v).13. The composition of any of to wherein the LPS extraction solution comprises chloroform claim 11 , methanol and water.14. The composition of any of to wherein the LPS extraction composition comprises an alkane claim 11 , ethanol and water.15. A composition according to any of to for use in the extraction of LPS from gram negative bacterial cells.16. Use of an LPS extraction composition ...

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Номер записи: 4

18-04-2013 дата публикации

SIALIC ACID DERIVATIVES

Номер: US20130096294A1

Автор: Jain Sanjay, PAPAIOANNOU Ioannis, THOBHANI Smita

Принадлежит: Lipoxen Technologies Limited

An amine or hydrazide derivative of a sialic acid unit, e.g. in a polysaccharide, is reacted with a bifunctional reagent at least one of the functionalities of which is an ester of N-hydroxy succinimide, to form an amide or hydrazide product. The product has a useful functionality, which allows it to be conjugated, for instance to proteins, drugs, drug delivery systems or the like. The process is of particular utility for derivatising amine groups introduced in sialic acid terminal groups of polysialic acids. 122-. (canceled)25. A compound according to wherein Ris selected from the group consisting of alkanediyl claim 23 , arylene claim 23 , alkarylene claim 23 , heteroarylene and alkylheteroarylene claim 23 , any of which is optionally interrupted by carbonyl claim 23 , ester claim 23 , sulfide claim 23 , ether claim 23 , amide and/or amine linkages.26. A compound according to wherein Ris C-Calkanediyl.27. A compound according to claim 23 , wherein Ris an oligo or poly-saccharide.2838-. (canceled)39. A compound according to wherein Ris selected from the group consisting of alkanediyl claim 24 , arylene claim 24 , alkarylene claim 24 , heteroarylene and alkylheteroarlene claim 24 , any of which is optionally interrupted by carbonyl claim 24 , ester claim 24 , sulfide claim 24 , ether claim 24 , amide and/or amine linkages.40. A compound according to wherein Ris Calkanediyl.41. A compound according to wherein Ris oligo- or poly-sialic acid.42. A compound according to wherein R′ is an oligo- or poly-sialic acid.45. The compound according to claim 23 , wherein is substituted alkarylene.48. The compound according to claim 24 , wherein Ris substituted alkarylene. This application is a divisional of U.S. application Ser. No. 11/660,128 having an international filing date of 12 Aug. 2005, which is the national phase of PCT application PCT/GB2005/003160 having an international filing date of 12 Aug. 2005, which claims priority from European application EP 05251015.3 filed ...

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Номер записи: 5

02-05-2013 дата публикации

Glucosamine materials

Номер: US20130109808A1

Автор: Jennifer H. Elisseeff

Принадлежит: JOHNS HOPKINS UNIVERSITY

Polymers comprising glucosamine (GlcN) are used to make medical devices. Examples include polyGlcN and carrier molecules containing multiple GlcN residues.

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Номер записи: 6

16-05-2013 дата публикации

PROCESS FOR MODIFYING THE PROPERTIES OF CITRUS PULP

Номер: US20130123374A1

Автор: GUSEK Todd Walter, MAZOYER Jacques André Christian, REEDER David Hiram, WALLECAN Joel Rene Pierre

Принадлежит: CARGILL INCORPORATED

A process is disclosed for modifying citrus fiber. Citrus fiber is obtained having a c* close packing concentration value of less than 3.8 w %, anhydrous basis. The citrus fiber can have a viscosity of at least 1000 mPa·s, wherein said citrus fiber is dispersed in standardized water at a mixing speed of from 800 rpm to 1000 rpm, to a 3 w/w % citrus fiber/standardized water solution, and wherein said viscosity is measured at a shear rate of 5 s−1 at 20 C. Citrus fiber can be obtained having a CIELAB L* value of at least 90. The citrus fiber can be used in food products, feed products, beverages, personal care products, pharmaceutical products or detergent products. 118.-. (canceled)19. A method of modifying the characteristics of a citrus fiber , the method comprising:a. hydrating the citrus fiber;b. treating the hydrated citrus fiber to obtain a homogenized citrus fiber;c. washing the homogenized citrus fiber with an organic solvent to obtain organic solvent washed citrus fiber;d. desolventizing and drying the organic solvent washed citrus fiber; ande. recovering modified citrus fiber therefrom.20. The method of claim 19 , wherein said citrus fiber is obtained from the group consisting of citrus pulp claim 19 , citrus peel claim 19 , citrus rag claim 19 , and combinations thereof.21. The method of claim 19 , wherein the viscosity of the modified citrus fiber is increased by at least 100% claim 19 , wherein the citrus fiber is dispersed in standardized water at a mixing speed of from 800 rpm to 1000 rpm claim 19 , to a 3 w/w % citrus fiber/standardized water solution claim 19 , and wherein the viscosity is measured at a shear rate of 5 sat 20° C.22. The method of claim 19 , wherein treating comprises pressure homogenization using a pressure of from 50 bar to 1000 bar.23. The method of claim 22 , wherein treating is a single-pass pressure homogenization using a pressure of from 300 bar to 1000 bar.24. The method of claim 22 , wherein treating is a multi-pass pressure ...

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Номер записи: 7

23-05-2013 дата публикации

Method of treating plant biomass

Номер: US20130130328A1

Автор: Haruo Takahashi, Kazuhide Tabata, Nobuhiro Ishida, Risa Nakamura, Satoshi Katahira

Принадлежит: Toyota Motor Corp

Plant biomass is immersed in a solution that contains a polar solvent and an imidazolium salt that has a melting point of at least 100° C. As a result, the cellulose and hemicellulose present in the plant biomass are relaxed (decrystallized and depolymerized) and brought into an easy-to-degrade state. Reacting the immersed plant biomass with a cellulase produces saccharide at a high conversion efficiency.

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Номер записи: 8

23-05-2013 дата публикации

Process for obtaining citrus fiber from citrus pulp

Номер: US20130131012A1

Автор: David Hiram Reeder, Jacques André Christian MAZOYER, Joël René Pierre Wallecan, Todd Walter Gusek

Принадлежит: Cargill Inc

A process is disclosed for obtaining citrus fiber from citrus pulp. Citrus fiber is obtained having a c* close packing concentration value of less than 3.8. The citrus fiber can be obtained having a viscosity of at least 1000 mPa·s, wherein said citrus fiber is dispersed in standardized water at a mixing speed of from 800 rpm to 1000 rpm, to a 3 w/w % citrus fiber/standardized water solution, and wherein said viscosity is measured at a shear rate of 5 s−1 at 20° C. Citrus fiber can be obtained having a CIELAB L* value of at least 90. The citrus fiber can be used in food products, feed products, beverages, personal care products, pharmaceutical products or detergent products.

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Номер записи: 9

23-05-2013 дата публикации

METHOD FOR PRODUCTION OF CELLULOSE NANO CRYSTALS FROM CELLULOSE-CONTAINING WASTE MATERIAL

Номер: US20130131332A1

Автор: Heyman Arnon, Lapidot Shaul, Meirovitch Sigal, Nevo Yuval, Rivkin Amit, Shoseyov Oded

Принадлежит: YISSUM Research Development Company of the Hebrew University of Jerusalem LTD.

A process is disclosed for recovering pure cellulose from a cellulose-containing sludge, the process comprising treating a sludge cellulose source under conditions permitting dissolution of non-cellulose material and suspension of the cellulose, wherein said dissolution conditions do not alter cellulose morphology. 1. A process for recovering pure cellulose from a cellulose-containing sludge , the process comprising treating a sludge cellulose source under conditions permitting dissolution of non-cellulose material and suspension of the cellulose , wherein said dissolution conditions do not alter cellulose morphology.2. The process according to claim 1 , wherein said sludge cellulose source contains between about 5% and about 60% cellulose claim 1 , or between about 40% and about 60% cellulose.3. The process according to claim 1 , wherein said sludge cellulose source is paper mill sludge.47-. (canceled)8. The process according to claim 1 , for the removal of about 95% claim 1 , by weight claim 1 , of calcium carbonate from the sludge cellulose source.9. (canceled)10. (canceled)11. The process according to claim 1 , wherein said conditions permitting dissolution of a non-cellulose material and suspension of the cellulose include treatment of said sludge cellulose source with a dilute acid.12. The process according to claim 11 , wherein the dilute acid having a concentration of between 0.1M and 1M acid.1317-. (canceled)18. The process according to claim 11 , wherein the acid concentration is between 0.1M and 0.3M.19. The process according to claim 11 , wherein said acid is selected from organic and inorganic acids claim 11 , said acid does not form water-insoluble salts with calcium carbonate.20. The process according to claim 19 , wherein said acid is selected from HCl claim 19 , HBr claim 19 , HPO claim 19 , and HNO.21. (canceled)22. (canceled)23. The process according to claim 20 , wherein said acid is HCl.2431-. (canceled)32. The process according to claim 11 , ...

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Номер записи: 10

04-07-2013 дата публикации

Isolation and deglycosylation of glycoproteins

Номер: US20130171658A1

Автор: Scott P. Fulton, Steven M. Murphy

Принадлежит: ProZyme Inc

The invention provides more rapid and cost-effective methods of deglycosylating target glycoproteins. In methods of the invention, the target glycoprotein is isolated from initial samples, which may contain multiple other glycoproteins, by subjecting the initial sample to a solid phase containing an affinity ligand, such as a deglycosylated antibody, that interacts specifically with the target glycoprotein. Once separated from the sample, the target glycoprotein can be deglycosylated in situ, or eluted from the solid phase, quantitated, and then deglycosylated.

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Номер записи: 11

04-07-2013 дата публикации

Process for the simultaneous substitution and crosslinking of a polysaccharide via its hydroxyl functional groups

Номер: US20130172288A1

Автор: BON BETEMPS Jeremie, PIRON Estelle

Принадлежит: LABORATOIRES VIVACY

A process for the simultaneous substitution and crosslinking of a polysaccharide via its hydroxyl functional groups, in an aqueous phase, which includes the following steps: 1. A process for the simultaneous substitution and crosslinking of a polysaccharide via its hydroxyl functional groups , in an aqueous phase , comprising the following steps:a polysaccharide is placed in an aqueous medium,it is brought into the presence of at least one precursor of a substituent,it is brought into the presence of a crosslinking agent,the substituted and crosslinked polysaccharide is obtained and isolated,{'sup': '−7', 'wherein, said process is carried out in the presence of a basic or acidic catalyst, the concentration of which is between 3.16×10and 0.32 mol/L, and at a temperature of less than 60° C.'}3. The process as claimed in claim 2 , wherein the reactive catalyst ratio (RCR) is between 0.2:1 and 3:1.4. The process as claimed in claim 1 , wherein the catalyst is a base.5. The process as claimed in claim 4 , wherein the base is an inorganic base chosen from sodium hydroxide or potassium hydroxide and wherein the reactive functional group of the catalyst is the OH ion.6. The process as claimed in claim 5 , wherein the concentration by weight of the inorganic base is between 1.2×10% and 1.15%.7. The process as claimed in claim 4 , wherein the concentration of catalyst HO is between 10mol/L and 0.32 mol/L claim 4 , such that 10mol/L≦[HO]≦0.32 mol/L.8. The process as claimed in claim 4 , wherein the pH of the aqueous reaction medium is basic and is between 8.5 and 13.5.9. The process as claimed in claim 1 , wherein the catalyst is an acid.10. The process as claimed in claim 9 , wherein the acid is an inorganic acid and is hydrochloric acid and the reactive functional group of the catalyst is the HO ion.11. The process as claimed in claim 9 , wherein the concentration by weight of the inorganic acid is between 1.14×10% and 1.3%.12. The process as claimed in claim 9 , wherein the ...

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Номер записи: 12

04-07-2013 дата публикации

REDUCTION OF ENDOTOXIN IN POLYSIALIC ACIDS

Номер: US20130172537A1

Автор: GREGORIADIS Gregory, Jain Sanjay, Laing Peter

Принадлежит:

The present invention relates to process for reducing the endotoxin content of a sample of fermentation broth containing polysialic acid and endotoxin comprising the sequential steps: (i) adding to the sample a base having a pKa of at least 12 to form a basic solution having a pH of at least 12, incubating the solution for a pre-determined time at a pre-determined temperature; and (ii) recovery of PSA, suitably by (iii) passing the sample through an anion-exchange column whereby polysialic acid is absorbed on the ion exchange resin; (iv) washing the column with one washing buffer, whereby polysialic acid remains absorbed on the ion exchange resin; and (v) eluting the polysialic acid from the column using an elution buffer to provide a product solution of polysialic acid having reduced endotoxin content. 1. A process for reducing the endotoxin content of a sample containing polysialic acid (PSA) and endotoxin comprising the steps of: (i) adding to the sample a base having a pKa of at least 12 to form a basic solution having a pH of at least 12 , (ii) incubating the solution for a predetermined time at a pre-determined temperature; and (iii) recovering polysialic acid having reduced endotoxin content.2. The process of wherein the base has a pKa of at least 13.3. The process of wherein the pH of the said basic solution is at least 13.4. The process of wherein the base is NaOH claim 1 , KOH claim 1 , Ca(OH).sub.2 or LiOH.5. The process of wherein the base is 2N NaOH.6. The process of claim 1 , wherein the pre-determined temperature is in the range 0 to 60° C.7. The process of in which step (iii) includes the following sequential substeps: (a) passing the sample through an anion-exchange column whereby polysialic acid is adsorbed on the ion exchange resin; (b) washing the column with a washing buffer claim 1 , whereby polysialic acid remains adsorbed on the ion exchange resin; and (c) eluting the polysialic acid from the column using an elution buffer to provide a ...

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Номер записи: 13

18-07-2013 дата публикации

Mixed Feedstocks Processing Using an Ionic Liquid

Номер: US20130183739A1

Автор: ARORA Rohit, Li Chenlin, Mathews Ian P., Simmons Blake A., Singh Seema

Принадлежит:

The present invention provides for a composition comprising two or more feedstocks and an ionic liquid (IL). The present invention also provides for a method for treating feedstocks, comprising providing a composition of the present invention comprising two or more feedstocks and an ionic liquid (IL). 1. A composition comprising two or more feedstocks and an ionic liquid (IL).2. The composition of claim 1 , wherein the two or more feedstocks comprises a softwood feedstock claim 1 , hardwood feedstock claim 1 , grass feedstock claim 1 , or agricultural feedstock.3. The composition of claim 2 , wherein the two or more feedstocks are chosen from a group consisting of softwood feedstock claim 2 , hardwood feedstock claim 2 , grass feedstock claim 2 , and agricultural feedstock.4. The composition of comprising three or more feedstocks.5. The composition of claim 1 , wherein the composition has a temperature from about room temperature to about 200 ° C.6. The composition of claim 1 , further comprising one of more cellulases claim 1 , or functional variant thereof.7. A method for treating feedstocks claim 1 , comprising providing a composition of the present invention comprising two or more feedstocks and an ionic liquid (IL).8. The method of claim 7 , wherein the providing step comprises adding or mixing two or more feedstocks and an IL to a solution to form the composition.9. The composition of claim 7 , wherein the two or more feedstocks comprises a softwood feedstock claim 7 , hardwood feedstock claim 7 , grass feedstock claim 7 , or agricultural feedstock.10. The composition of claim 9 , wherein the two or more feedstocks are chosen from a group consisting of softwood feedstock claim 9 , hardwood feedstock claim 9 , grass feedstock claim 9 , and agricultural feedstock.11. The method of claim 7 , further comprising incubating the composition for equal to or more than 1 h claim 7 , 2 h claim 7 , or 3 h.12. The method of claim 7 , further comprising introducing to the ...

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Номер записи: 14

08-08-2013 дата публикации

AQUEOUS IRON CARBOHYDRATE COMPLEXES, THEIR PRODUCTION AND MEDICAMENTS CONTAINING THEM

Номер: US20130203698A1

Автор: Geisser Peter, Philipp Erik, Richle Walter

Принадлежит: VIFOR (INTERNATIONAL) AG.

Water soluble Iron carbohydrate complex obtainable from an aqueous solution of iron(III) salt and an aqueous solution of the oxidation product of one or more maltrodextrins using an aqueous hypochlorite solution at a pH-value within the alkaline range, where, when one maltodextrin is applied, its dextrose equivalent lies between 5 and 20, and when a mixture of several maltodextrins is applied, the dextrose equivalent of the mixture lies between 5 and 20 and the dextrose equivalent of each individual maltodextrin contained in the mixture lies between 2 and 40, process for its production and medicament for the treatment and prophylaxis of iron deficiency conditions. 111-. (canceled)12. An iron carboxypolymaltose complex wherein said iron carboxypolymaltose complex is polynuclear iron (III)-hydroxide 4(R)-(poly-(1→4)-O-α-glucopyranosyl)-oxy-2(R) ,3(S) ,5(R) ,6-tetrahydroxy-hexanoate and has a weight average molecular weight in the range of from 80 kDa to 400 kDa.13. The iron carboxypolmaltose complex of claim 12 , having a weight average molecular weight in the range of from 80 kDa to 350 kDa.14. The iron carboxypolymaltose complex of claim 12 , having a weight average molecular weight in the range of from 80 kDa to 300 kDa.15. The iron carboxypolymaltose complex of claim 12 , having a weight average molecular weight in the range of from 118 kDa to 270 kDa.16. The iron carboxypolymaltose complex of claim 12 , having a weight average molecular weight of about 150 claim 12 ,000 Da.17. The iron carboxypolymaltose complex of claim 12 , having a weight average molecular weight of about 271 claim 12 ,000 Da.18. The iron carboxypolymaltose complex of claim 12 , having a weight average molecular weight of about 141 claim 12 ,000 Da.19. The iron carboxypolymaltose complex of claim 12 , having a weight average molecular weight of about 140 claim 12 ,000 Da.20. The iron carboxypolymaltose complex of claim 12 , having a weight average molecular weight of about 189 claim 12 ,000 Da ...

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Номер записи: 15

29-08-2013 дата публикации

Process for obtaining chondroitin sulphated at the 4- or 6- positions of n-acetyl-galactosamine residues

Номер: US20130225802A1

Автор: Annalida Di Nola, Chiara Schiraldi, Cristina De Castro, Emiliano Bedini, Mario De Rosa, Michelangelo Parrilli, Odile Francesca Restaino

Принадлежит: Altergon SA

Disclosed is a process for the production of chondroitin sulphate, wherein N-acetyl-galactosamine residues sulphated at the 4- or 6-positions are present on the same polysaccharide chain.

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Номер записи: 16

19-09-2013 дата публикации

Binders and Materials Made Therewith

Номер: US20130244524A1

Автор: Brian Lee Swift, Ronald E. Kissell, Ruijian Xu

Принадлежит: Knauf Insulation GmbH USA

A curable aqueous composition is disclosed comprising a carbohydrate, a crosslinking agent, and an amine base, wherein the curable aqueous composition has a pH adjusted by the amine base. Further disclosed is a method of forming a curable aqueous solution.

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Номер записи: 17

03-10-2013 дата публикации

USE OF POLYSACCHARIDES FROM RADIX ISATIDIS IN MANUFACTURE OF MEDICAMENTS AGAINST INFLUENZA VIRUS

Номер: US20130259809A1

Автор: GUAN Wenda, Li Chuyuan, Lin Qing, MO Ziyao, QIN Sheng, WANG Deqin, WANG Yutao, Wang Zheng, YANG Zifeng, ZHAO Suishan, ZHONG Nanshan

Принадлежит:

A process of using root polysaccharide in the manufacture of medicaments for treating and/or preventing diseases induced by influenza virus and complications thereof, wherein the molecular weight of the root polysaccharide is 3000-7000 Da, and the influenza viruses include influenza virus A, influenza virus B, avian influenza virus, such as human influenza virus H1N1, H3N2, avian influenza virus H6N2, H7N3, H9N2 and INF B. The mechanism of the root polysaccharide against influenza virus is due to the ability to inhibit the attachment of influenza virus to the host cell. The present invention also discloses a pharmaceutical composition containing the above-mentioned root polysaccharides and a method for preparing the polysaccharides. 1IsatisIsatis. A process in preventing and/or treating diseases caused by influenza viruses and complications thereof , using root polysaccharide , wherein the molecular weight of the root polysaccharide is 3000-7000 Dalton.2IsatisRadix Isatidis. The process according to claim 1 , wherein the root polysaccharide is extracted from raw materials according to a method comprising:{'i': 'Radix Isatidis', 'A. decocting the raw materials by adding suitable times of distilled water to produce a decocted fluid, concentrating the decocted fluid to produce a concentrated solution so that the baume degree of which is 18-20° Bé at 50° C.;'}B. cooling the concentrated solution obtained in Step A to below 45° C., adding alcohol to produce an alcohol solution so that alcohol content is up to 60% or above 60% in the alcohol solution, allowing the alcohol solution to stand for more than 12 hours to precipitate;C. passing the alcohol solution through a macroporous resin column, eluting with pure water, and collecting an eluent;D. deproteinizing the eluent, and placing the deproteinized solution in a dialysis bag with a proper molecular weight to dialyse; and{'i': 'Isatis', 'E. evaporating the solution inside and/or outside the dialysis bag with a rotary ...

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Номер записи: 18

07-11-2013 дата публикации

NON-REDUCING END MODIFIED GLUCAN, METHOD FOR PRODUCING SAME, AND USE THEREOF

Номер: US20130295163A1

Автор: Kakutani Ryo, KUBO Akiko, Sambe Haruyo, Takaha Takeshi, Yanase Michiyo

Принадлежит: EZAKI GLICO CO., LTD.

An object of the present invention is to provide a glucan containing at least one residue selected from an N-acetylglucosamine residue and a galactose residue, and a modified product. The branched glucan of the present invention is a branched glucan wherein the branched glucan has a plurality of non-reducing ends and at least one residue selected from an N-acetylglucosamine residue and a galactose residue is bound via an α-1,4-bond to each of two or more non-reducing ends of the branched α-1,4-glucan, but neither an N-acetylglucosamine residue nor a galactose residue is present at the position other than the non-reducing ends of the branched α-1,4-glucan. 1. A branched glucan ,wherein the branched glucan has a plurality of non-reducing ends, andat least one residue selected from an N-acetylglucosamine residue and a galactose residue is bound via an α-1,4-bond to each of two or more non-reducing ends of the branched α-1,4-glucan,but neither an N-acetylglucosamine residue nor a galactose residue is present at the position other than the non-reducing ends of the branched α-1,4-glucan, and{'sup': '5', 'the degree of polymerization of the branched α-1,4-glucan is 15 or more and 4×10or less.'}2. The branched glucan according to claim 1 , wherein the branched α-1 claim 1 ,4-glucan is selected from the group consisting of a branched maltooligosaccharide claim 1 , starch claim 1 , amylopectin claim 1 , glycogen claim 1 , dextrin claim 1 , enzymatically synthesized branched glucan and highly branched cyclic glucan.3. A hydroxyl group-modified product of the branched glucan according to claim 1 , wherein the modification on the hydroxyl group is a modification on some or all of alcoholic hydroxyl groups of the glucan claim 1 , and the modification on the hydroxyl group is independently selected from the group consisting of hydroxyalkylation claim 1 , alkylation claim 1 , acetylation claim 1 , carboxymethylation claim 1 , sulfation and phosphorylation.4. A reducing end-modified ...

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Номер записи: 19

21-11-2013 дата публикации

Aqueous Diphase Solvent System for Preparing High-Purity Polysaccharides

Номер: US20130310554A1

Автор: HAN Quanbin

Принадлежит: HONG KONG BAPTIST UNIVERSITY

The present invention provides an aqueous diphase solvent system for an one-step separation of high-purity polysaccharide from a mixture. In particular, the present invention provides method of purifying and isolating high molecular weight polysaccharides in plant with good homogeneity. 1. A method for purifying and isolating polysaccharide from a natural product comprising preparing an aqueous diphase solvent system comprising 12-18% w/v PEG and 12-18% w/v MgSO ,mixing said aqueous diphase solvent system with said natural product to be separated to form a second mixture; and{'sub': '4', 'separating the second mixture by means of solvent separation, wherein molecular weight of the PEG ranges from 1,000-3,400 and ratio of PEG to MgSOis 2 to 3:3 to 2.'}2. The method of wherein the means of solvent separation is selected from the group consisting of solvent partition claim 1 , counter-current distribution claim 1 , cross-current extraction claim 1 , counter-current extraction claim 1 , counter-current chromatography or liquid-to-liquid separation.3. The method of claim 1 , wherein the natural product is a herb.4. The method of claim 1 , further comprising preparing an extract of the natural product.5. The method of claim 4 , wherein the extract is water extract. The present application claims priority of U.S. provisional application No. 61/648,620 filed May 18, 2012, and which the disclosure is hereby incorporated by reference.The present invention is in the field of pharmaceuticals and chemical industries. In particular, this invention relates to an aqueous diphase solvent system for high-purity polysaccharides purification and isolation. The present invention also provides methods for purifying and isolating polysaccharide in natural products.Polysaccharides exhibit a variety of biological activities such as anticancer, antiviral, immuno-stimulatory activities and have a long history of practical application in our daily life. A lot of botanical materials used in ...

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Номер записи: 20

26-12-2013 дата публикации

OLIGOSACCHARIDES AND OLIGOSACCHARIDE-PROTEIN CONJUGATES DERIVED FROM CLOSTRIDIUM DIFFICILE POLYSACCHARIDE PS-II, METHODS OF SYNTHESIS AND USES THEREOF, IN PARTICULAR AS A VACCINE

Номер: US20130344104A1

Автор: Hecht Marie-Lyn, Oberli Matthias, Seeberger Peter H.

Принадлежит:

The present invention provides an oligosaccharide-protein conjugate comprising an oligosaccharide, in particular synthetic oligosaccharide, derived from the repeating unit of the glycopolymer PS-II and a protein carrier. More specifically, the oligosaccharide is the hexasaccharide having the following formula (I) wherein R is a linker or spacer group. In a specific embodiment of the invention, R is (CH2)NH, with n being an integer from 2 to 50. The present invention also provides the use of said oligosaccharide and said oligosaccharide-protein conjugate for the treatment or prevention of a disease caused by the pathogen In still further aspects, the present invention also provides a favourable method for preparing said oligosaccharide and said oligosaccharide-protein conjugate. 1Clostridium difficile. An oligosaccharide-protein conjugate comprising an oligosaccharide representing part of a repeating unit of the glycopolymer PS-II and a protein carrier.3. The oligosaccharide-protein conjugate according to claim 2 , wherein R is an aliphatic or aromatic residue comprising a reactive functional group.4. The oligosaccharide-protein conjugate according to claim 3 , wherein R is (CH)NH claim 3 , with n being an integer from 2 to 50.5. The oligosaccharide-protein conjugate according to claim 1 , wherein the protein carrier is selected from the group consisting of diphtheria toxoid Crm claim 1 , tetanus toxoid claim 1 , outer membrane protein (OMP) claim 1 , bovine serum albumin claim 1 , and keyhole limpet hemocyanine6. The oligosaccharide-protein conjugate according to claim 2 , wherein the protein carrier is diphtheria toxoid Crm.8. The hexasaccharide according to claim 7 , wherein R is (CH)NH claim 7 , with n being an integer from 2 to 50.10Clostridium difficile.. The hexasaccharide according to claim 7 , which is effective to treat or prevent a disease caused by the pathogen11Clostridium difficile.. The oligosaccharide-protein conjugate according to claim 1 , which is ...

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Номер записи: 21

16-01-2014 дата публикации

Bio-based Fiber Gums (BFGs) and Processes for Producing BFGs

Номер: US20140017376A1

Автор: Hanah Kyle A., Hicks Kevin B., Johnston David, Shukla Triveni P., Yadav Madhav P.

Принадлежит: The United States of America, as represented by the Secretary of Agriculture

Processes for the preparation of bio-based fiber gums and products produced by these processes and some of their uses. 1. A process for the preparation of bio-based fiber gums comprising:(a) mixing agricultural materials with a heated alkaline solution at temperatures in the range of about 75° to about 150° C. to form a slurry;(b) separating out the insoluble components from said slurry to yield a solution having a pH of about 9 to about 14 wherein said solution contains about 0.1 to about 10 wt % solids wherein said solids are alkaline soluble fractions;and one of the following:(c) evaporating said solution to about 16 to about 23 wt % solids and drying to a powder;(d) adjusting the pH of said solution to a pH of about 2 to about 12, evaporating said solution to about 16 to about 23 wt % solids and drying to a powder;(e) evaporating said solution to about 16 to about 23 wt % solids, adjusting the pH of said solution to a pH of about 2 to about 12 and drying to a powder;(f) evaporating said solution to about 16 to about 23 wt % solids and precipitating out said soluble components with about two to about five volumes of solvent to form a precipitate and a supernatant, and separately drying said precipitate and said supernatant;(g) evaporating said solution to about 16 to about 23 wt % solids, adjusting the pH of said solution to a pH of about 2 to about 12 and precipitating out said soluble components with one to five volumes of organic solvent to form a precipitate and a supernatant, and separately drying said precipitate and said supernatant;(h) adjusting the pH of said solution to a pH of about 2 to about 12, evaporating said solution to about 16 to about 23 wt % solids and precipitating out said soluble components with about one to five volumes of organic solvent to form a precipitate and a supernatant, and separately drying said precipitate and said supernatant; or(i) adjusting the pH of said solution to a pH of about 2 to about 5 to precipitate Hemicellulose A ...

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Номер записи: 22

20-03-2014 дата публикации

Silk Protein Composite Coating Solution and its Preparation Method and Application

Номер: US20140076195A1

Автор: Yaoqi Jin

Принадлежит: Individual

Silk protein composite coating solution and its preparation method and application. In weight percent, silk protein complex coating liquid is constituted by the following substances: 2 to 10% of the crosslinking agent, 0.5 to 2% acetic acid, 10 to 20% of the silk protein, the remainder being water. The method for preparation of silk protein composite coating solution has these steps: (1) preparation of the silk protein; (2) silk protein composite coating solution was prepared. Silk protein of the present invention has a natural emollient, antibacterial, and anti-allergic effects, and can be used on daily sanitary supplies for women, infants, and elderlies.

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Номер записи: 23

27-03-2014 дата публикации

Glucosyltransferase enzymes for production of glucan polymers

Номер: US20140087431A1

Автор: Hongxian He, Mark S. Payne, Thomas Scholz, Yefim Brun

Принадлежит: EI Du Pont de Nemours and Co

Reaction solutions are disclosed herein comprising water, sucrose and a glucosyltransferase enzyme that synthesizes poly alpha-1,3-glucan. The glucosyltransferase enzyme can synthesize insoluble glucan polymer having at least 50% alpha-1,3 glycosidic linkages and a number average degree of polymerization of at least 100. Further disclosed are methods of using such glucosyltransferase enzymes to produce insoluble poly alpha-1,3-glucan.

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Номер записи: 24

04-01-2018 дата публикации

OLIGOSACCHARIDE COMPOSITIONS FOR USE IN NUTRITIONAL COMPOSITIONS, AND METHODS OF PRODUCING THEREOF

Номер: US20180000146A1

Автор: GEREMIA John M.

Принадлежит:

Described herein are methods of producing prebiotic compositions that are made up of oligosaccharide compositions, as well as methods of using such prebiotic compositions in nutritional compositions and methods of producing such oligosaccharide and nutritional compositions. 1. A method of producing a prebiotic composition , comprising: wherein the catalyst comprises acidic monomers and ionic monomers connected to form a polymeric backbone, or', 'wherein the catalyst comprises a solid support, acidic moieties attached to the solid support, and ionic moieties attached to the solid support; and, 'combining feed sugar with a catalyst to form a reaction mixture, wherein the catalyst comprises acidic moieties and ionic moieties,'}producing a prebiotic composition from at least a portion of the reaction mixture2. The method of claim 1 , wherein the catalyst comprises acidic monomers and ionic monomers connected to form a polymeric backbone.3. The method of claim 2 , wherein each acidic monomer independently comprises at least one Bronsted-Lowry acid.4. The method of or claim 2 , wherein each ionic monomer independently comprises at least one nitrogen-containing cationic group claim 2 , at least one phosphorous-containing cationic group claim 2 , or a combination thereof.5. The method of claim 1 , wherein the catalyst comprises a solid support claim 1 , acidic moieties attached to the solid support claim 1 , and ionic moieties attached to the solid support.6. The method of claim 5 , wherein the solid support comprises a material claim 5 , wherein the material is selected from the group consisting of carbon claim 5 , silica claim 5 , silica gel claim 5 , alumina claim 5 , magnesia claim 5 , titania claim 5 , zirconia claim 5 , clays claim 5 , magnesium silicate claim 5 , silicon carbide claim 5 , zeolites claim 5 , ceramics claim 5 , and any combinations thereof.7. The method of or claim 5 , wherein each acidic moiety independently has at least one Bronsted-Lowry acid.8. The ...

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Номер записи: 25

03-01-2019 дата публикации

Streptococcus pneumoniae capsular polysaccharides and conjugates thereof

Номер: US20190000953A1

Автор: Avvari Krishna PRASAD, Jianxin Gu, Jin-Hwan Kim, Rajesh Kumar Kainthan, Yu-Ying Yang

Принадлежит: PFIZER INC

The invention relates to activated Streptococcus pneumoniae serotype 10A, 22F or 33F polysaccharides and processes for their preparation. The invention also relates to immunogenic conjugates comprising Streptococcus pneumoniae serotype 10A, 22F or 33F polysaccharides covalently linked to a carrier protein, processes for their preparation and immunogenic compositions and vaccines comprising them.

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Номер записи: 26

07-01-2016 дата публикации

POLYSACCHARIDE POWDER AND ANTI-ADHESIVE MATERIAL CONTAINING THE SAME

Номер: US20160002362A1

Автор: NIIMI Taishi, YUKI Risa

Принадлежит: TERUMO KABUSHIKI KAISHA

The present invention provides a means for improving water-solubility of polysaccharide. A polysaccharide powder according to the present invention has a particle size distribution in which 30 vol or more of the total volume of the powder has particle sizes of 200 to 750 μm. 1. A polysaccharide-containing powder having a particle size distribution in which 30 vol % or more of the total volume of the powder has a particle size of 200 to 750 μm.2. The powder according to claim 1 , wherein a particle size at 10% of cumulative volume (D10) is 60 to 120 μm.3. The powder according to claim 1 , wherein a particle size at 90% of cumulative volume (D90) is 300 to 520 μm.4. The powder according to claim 1 , having a peak top of 100 to 500 μm.5. The powder according to claim 1 , wherein the polysaccharide has a molecular weight of 30 claim 1 ,000 to 120 claim 1 ,000.6. An anti-adhesive material claim 1 , comprising the powder set forth in . The present invention relates to a polysaccharide powder and an anti-adhesive material containing the same. More particularly, the present invention relates to improvement for increasing water-solubility of polysaccharide.Surgical operations in surgery may damage biological tissues. Exposure of biological tissues to the air by incisions has been known to dry or oxidize biological tissues, resulting in damaged biological tissues. The damaged tissues may cause postoperative inflammation or the like to induce the adhesion between tissues that should be naturally separated. Such postoperative adhesion between tissues may lead to, for example, serious complications, such as ileus and infertility, in an abdomen. Various types of anti-adhesive materials have thus been developed to cover damaged sites of biological tissues for the prevention of adhesion.Currently-known anti-adhesive materials have been mainly composed of biological polymer materials, such as polysaccharides and polypeptides, which are unlikely to cause adverse effects on living ...

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Номер записи: 27

07-01-2016 дата публикации

Process For Filtration Of Homopolysaccharides

Номер: US20160002363A1

Автор: Freyer Stephan, Käppler Tobias, Leonhardt Bernd, Rollie Sascha, Therre Jörg, VOß Hartwig

Принадлежит:

The present invention relates to an improved process for filtering aqueous fermentation broths comprising glucans and biomass using symmetrical tubular membranes. 1. A process for separating off an aqueous solution of glucans from an aqueous fermentation broth comprising glucans and biomass in a filtration plant , which comprises at least the following steps:a) introducing a feed stream comprising the aqueous fermentation broth into the filtration plant,b) passing the feed stream through at least one symmetrical tubular membrane which has a cylindrical shape and has pores,c) taking off a permeate stream comprising the aqueous solutions of glucans,wherein the symmetrical tubular membrane has an internal diameter in the range from ≧2 mm to ≦6 mm.2. The process according to claim 1 , wherein the symmetrical tubular membrane has an internal diameter in the range from ≧3 mm to ≦6 mm.3. The process according to claim 1 , wherein the glucans comprise a main chain composed of β-1 claim 1 ,3-glycosidically linked glucose units and side groups which are composed of glucose units and are β-1 claim 1 ,6-glycosidically bound to the main chain.4. The process according to claim 1 , wherein the symmetrical tubular membrane has pores having a d90 pore size in the range from ≧4 μm to ≦45 μm determined in accordance with ISO 15901-1.5. The process according to claim 1 , wherein the length of the symmetrical tubular membrane is in the range from ≧0.2 m to ≦1.5 m.6. The process according to claim 1 , wherein the feed stream is conveyed claim 1 , in step b) claim 1 , at a flow velocity over the membrane in the range from ≧0.5 m/s to ≦5 m/s.7. The process according to claim 1 , wherein the symmetrical tubular membrane has a wall thickness in the range from ≧0.3 mm to ≦3 mm.8. The process according to claim 1 , wherein the symmetrical tubular membrane is made of a material which has a separation limit in the range from ≧0.5 to ≦45 μm claim 1 , determined in accordance with ASTM F 795.9. ...

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Номер записи: 28

05-01-2017 дата публикации

COMPOSITIONS AND METHODS FOR IMPROVING REBAUDIOSIDE X SOLUBILITY

Номер: US20170002034A1

Автор: CHATURVEDULA VENKATA SAI PRAKASH, Ma Gil, MARKOSYAN Avetik, Prakash Indra

Принадлежит:

Polymorphic and amorphous forms of Rebaudioside X and methods for preparing the same are provided herein. Also provided herein are Rebaudioside X complexes and methods for preparing the same. Sweetener compositions and sweetened compositions comprising Rebaudioside X forms and Rebaudioside X complexes are described, as well as and methods of their preparation. Methods of improving the flavor and/or temporal profile of sweetenable compositions, such as beverages, are also provided herein. 1. A method for preparing amorphous Rebaudioside X comprising:(i) heating a mixture comprising water and Rebaudioside X to a temperature between about 100° C. to about 130° C.(ii) cooling the mixture; and(iii) removing the solvent from the mixture to provide amorphous Rebaudioside X.2. The method of claim 1 , wherein the Rebaudioside X in (i) is Form A Rebaudioside X.3. The method of claim 1 , wherein the Rebaudioside X in (i) is Material E Rebaudioside X.4. (canceled)5. (canceled)6. The method of claim 1 , wherein the mixture is heated to a temperature from about 120° C. to about 125° C.762.-. (canceled)63. The method of claim 1 , wherein the mixture in (ii) is cooled to room temperature such that crash precipitation does not occur.64. The method of claim 63 , wherein the mixture is cooled at a rate of about 1° C. per minute.65. The method of claim 1 , wherein the solvent is removed by a process selected from the group consisting of decantation claim 1 , centrifugation claim 1 , filtration claim 1 , evaporation claim 1 , vacuum claim 1 , spray-drying and a combination thereof.66. The method of claim 65 , wherein the solvent is removed by spray-drying.67. The method of claim 1 , wherein the amorphous Rebaudioside X has a solubility of about 0.3% or greater.68. The method of claim 1 , wherein the amorphous Rebaudioside A has a solubility of about 1.0% or greater.69. A method for preparing amorphous Rebaudioside X comprising:(i) heating a mixture comprising ethanol and Rebaudioside X ...

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Номер записи: 29

05-01-2017 дата публикации

Methods for Purifying Polysaccharides and Pharmaceutical Compositions and Medical Devices Containing the Same

Номер: US20170002099A1

Автор: FORGET Aurelien, Shastri Venkatram Prasad, Vonwil Daniel

Принадлежит:

Methods for removing endotoxin from naturally occurring materials, such as polysaccharides (e.g., agarose and/or carrageenan) are described herein. Polysaccharides that are substantially free of endotoxins and uses thereof are also described. The polysaccharide materials can be isolated from microorganisms, multicellular organisms, such as, algae, plants, seaweed, etc. The method involves the use of acidic and basic solutions to hydrolyze the lipid-polysaccharide bond in endotoxins. Cleaving the fatty acid from the polysaccharide reduces the water-solubility of the fatty acid and enables its removal with an organic solvent such as ethanol. The polysaccharide component can also undergo acidic or basic hydrolysis due to the weak glycosidic bond between the sugar rings. 1. A method for isolating and purifying a naturally occurring agarose or derivative thereof produced from a biological source , the method comprising:(i) dispersing the agarose in one or more aliphatic alcohols to disrupt the bacterial wall to solubilize the lipid portion of endotoxins;(ii) removing the aliphatic alcohol to remove the lipid portion of the endotoxins and obtain the agarose or derivative thereof in solid form;(iii) dispersing the solid agarose or derivative thereof in a basic solution to hydrolyze lipid-inner core bonds of the endotoxins and solubilize the polysaccharide component of the endotoxins;(iv) washing the solution from step (iii) with an aliphatic alcohol to remove free lipids;(v) removing the basic solution in step (iii) to obtain the agarose or derivative thereof in solid form;(vi) dispersing the solid agarose or derivative thereof in an acidic solution to hydrolyze lipid-inner core bonds of the endotoxins not cleaved in step (iii);(vii) removing the acidic solution in step (vi) to obtain the agarose or derivative thereof in solid form;(viii) dispersing the solid agarose or derivative thereof from step (vii) in a second basic solution to further cleave lipid-inner core bonds ...

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Номер записи: 30

05-01-2017 дата публикации

Modified glucosyltransferases for producing branched alpha-glucan polymers

Номер: US20170002336A1

Автор: Mark S. Payne, Richard R. Bott, Yefim Brun

Принадлежит: DANISCO US INC, EI Du Pont de Nemours and Co

Glucosyltransferase enzymes are disclosed herein that produce branched alpha-glucan polymer. Also disclosed, for example, are polynucleotides encoding these enzymes, as well as methods of producing branched alpha-glucan polymer.

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Номер записи: 31

02-01-2020 дата публикации

AMPHIPHILIC POLYSACCHARIDE DERIVATIVES AND COMPOSITIONS COMPRISING SAME

Номер: US20200002646A1

Автор: FLITER Kristi Lynn, Huang Zhengzheng, Lu Helen S M, Qiu Weiming, Shah Mukesh C., Shuey Steven W., SIVIK Mark Robert

Принадлежит:

The disclosure relates to compositions comprising a polysaccharide derivative, wherein the polysaccharide derivative comprises a polysaccharide substituted with a) at least one hydrophobic group, and b) at least one hydrophilic group, wherein the polysaccharide is poly alpha-1,3-glucan, poly alpha-1,6-glucan, or poly alpha-1,3-1,6-glucan. 1. A composition comprising:a polysaccharide derivative, wherein the polysaccharide derivative comprises a polysaccharide substituted witha) at least one hydrophobic group; andb) at least one hydrophilic group;wherein the polysaccharide is poly alpha-1,3-glucan, poly alpha-1,6-glucan, or poly alph-1,3-1,6-glucan.2. The composition of claim 1 , wherein the poly alpha-1 claim 1 ,3-glucan comprises a backbone of glucose monomer units wherein greater than or equal to 50% of the glucose monomer units are linked via alpha-1 claim 1 ,3-glycosidic linkages.3. The composition of claim 1 , wherein the poly alpha-1 claim 1 ,3-glucan comprises a backbone of glucose monomer units wherein greater than or equal to 90% of the glucose monomer units are linked via alpha-1 claim 1 ,3-glycosidic linkages.4. The composition of claim 1 , wherein the poly alpha-1 claim 1 ,6-glucan comprises a backbone of glucose monomer units wherein greater than or equal to 40% of the glucose monomer units are linked via alpha-1 claim 1 ,6-glycosodic linkages.5. The composition of claim 1 , wherein the poly alpha-1 claim 1 ,6-glucan has a degree of alpha-1 claim 1 ,2-branching that is less than 50%.6. The composition of claim 1 , wherein the at least one hydrophobic group comprises a Cto Calkyl claim 1 , a Cto Calkene claim 1 , a Cto Calkyne claim 1 , a polyether comprising repeat units of (—CHCHO—) claim 1 , (—CHCH(CH)O—) claim 1 , or mixtures thereof claim 1 , wherein the total number of repeat units is in the range of from 3 to 100 claim 1 , a Cto Caryl claim 1 , a benzyl claim 1 , a C-Calkyl sulfonyl claim 1 , a C-Caryl sulfonyl claim 1 , a p-toluenesulfonyl group ...

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Номер записи: 32

01-01-2015 дата публикации

Glucosyltransferase enzymes for production of glucan polymers

Номер: US20150004651A1

Автор: Hongxian He, Mark S Payne, Thomas Scholz, Yefim Brun

Принадлежит: EI Du Pont de Nemours and Co

Reaction solutions are disclosed herein comprising water, sucrose and a glucosyltransferase enzyme that synthesizes poly alpha-1,3-glucan. The glucosyltransferase enzyme can synthesize insoluble glucan polymer having at least 50% alpha-1,3 glycosidic linkages and a number average degree of polymerization of at least 100. Further disclosed are methods of using such glucosyltransferase enzymes to produce insoluble poly alpha-1,3-glucan.

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Номер записи: 33

13-01-2022 дата публикации

METHODS FOR SYNTHESIZING ANTICOAGULANT POLYSACCHARIDES

Номер: US20220010344A1

Автор: GESTEIRA Tarsis Ferreira, LAJINESS Daniel H.

Принадлежит:

The present invention includes methods for preparing anticoagulant polysaccharides using several non-naturally occurring, engineered sulfotransferase enzymes that are designed to react with aryl sulfate compounds instead of the natural substrate, PAPS, to facilitate sulfo group transfer to polysaccharide sulfo group acceptors. Suitable aryl sulfate compounds include, but are not limited to, p-nitrophenyl sulfate or 4-nitrocatechol sulfate. Anticoagulant polysaccharides produced by methods of the present invention comprise N-, 3-O-, 6-O-sulfated glucosamine residues and 2-O sulfated hexuronic acid residues, have comparable anticoagulant activity compared to commercially-available anticoagulant polysaccharides, and can be utilized to form truncated anticoagulant polysaccharides having a reduced molecular weight. 1. A method of enzymatically synthesizing an N- , 2-O , 3-O , 6-O sulfated , heparan sulfate (N ,2 ,3 ,6-HS) product in the absence of 3′-phosphoadenosine 5′-phosphosulfate (PAPS) , the method comprising the following steps:a. providing a starting polysaccharide mixture comprising N-sulfated heparosan;b. combining the starting polysaccharide mixture with a first sulfotransferase reaction mixture comprising a hexuronyl 2-O sulfotransferase enzyme (2OST) and a sulfo group donor, the sulfo group donor consisting of an aryl sulfate compound, to form a first product mixture, the first product mixture comprising an N-, 2-O sulfated heparan sulfate (N,2-HS) product;c. combining the first product mixture with a second sulfotransferase reaction mixture comprising a glucosaminyl 6-O sulfotransferase enzyme (6OST) and a sulfo group donor, the sulfo group donor consisting of an aryl sulfate compound, to form a second product mixture, the second product mixture comprising an N-, 2-O, 6-O sulfated heparan sulfate (N,2,6-HS) product;d. combining the second product mixture with a third sulfotransferase reaction mixture comprising a glucosaminyl 3-O sulfotransferase enzyme ( ...

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Номер записи: 34

12-01-2017 дата публикации

Sialic Acid Derivatives For Protein Derivatisation And Conjugation

Номер: US20170007706A1

Автор: GREGORIADIS Gregory, Hreczuk-Hirst Dale Howard, Jain Sanjay, Laing Peter, PAPAIOANNOU Ioannis

Принадлежит: Lipoxen Technologies Limited

Derivatives are synthesized of starting materials, usually polysaccharides, having sialic acid at the reducing terminal end, in which the reducing terminal unit is transformed into an aldehyde group. Where the polysaccharide has a sialic acid unit at the non-reducing end it may be passivated, for instance by converting into hydroxyl-substituted moiety. The derivatives may be reacted with substrates, for instance containing amine or hydrazine groups, to form non-cross-linked polysialylated compounds. The substrates may, for instance, be therapeutically useful drugs peptides or proteins or drug delivery systems. 1. A process for producing an aldehyde derivative of a reducing terminal sialic acid of a polysialic acid , which process comprises:a) providing a starting material of polysialic acid having a terminal sialic acid unit at a non-reducing end which has a vicinal diol group, wherein the starting material is subjected to a selective oxidation to oxidize the vicinal diol group at the non-reducing end to an aldehyde;b) reduction to reductively open a ring at the reducing terminal sialic acid unit, whereby a vicinal diol group is formed and the aldehyde at the non-reducing end is also reduced to form a hydroxy group which is not part of a vicinal diol group;c) selective oxidation to oxidize the vicinal diol group to form an aldehyde group at the reducing terminal sialic acid; andd) conjugating the aldehyde group at the reducing terminal sialic acid to a substrate, wherein the substrate is a polypeptide or a protein.2. A process according to in which a preliminary oxidation step is carried out under conditions such that there is substantially no mid-chain cleavage of the polysaccharide chain.5. A compound of claim 3 , wherein the protein is insulin or growth hormone.6. A pharmaceutical composition comprising a compound according to and a pharmaceutical excipient. This application is a continuation of U.S. application Ser. No. 14/155,855, filed Jan. 15, 2014, now U.S. ...

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Номер записи: 35

08-01-2015 дата публикации

Glucosyltransferase enzymes for production of glucan polymers

Номер: US20150010956A1

Автор: Hongxian He, Mark S. Payne, Thomas Scholz, Yefim Brun

Принадлежит: EI Du Pont de Nemours and Co

Reaction solutions are disclosed herein comprising water, sucrose and a glucosyltransferase enzyme that synthesizes poly alpha-1,3-glucan. The glucosyltransferase enzyme can synthesize insoluble glucan polymer having at least 50% alpha-1,3 glycosidic linkages and a number average degree of polymerization of at least 100. Further disclosed are methods of using such glucosyltransferase enzymes to produce insoluble poly alpha-1,3-glucan.

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Номер записи: 36

08-01-2015 дата публикации

Processing biomass

Номер: US20150010967A1

Автор: Marshall Medoff, Thomas Craig Masterman

Принадлежит: Xyleco Inc

Biomass feedstocks (e.g., plant biomass, animal biomass, and municipal waste biomass) are processed to produce useful products, such as fuels. For example, systems are described that can use feedstock materials, such as cellulosic and/or lignocellulosic materials and/or starchy materials, to produce a product or intermediate, e.g., energy, a food, a fuel, or a material.

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Номер записи: 37

10-01-2019 дата публикации

Polysaccharide Purification for Vaccine Production using Lytic Enzymes, Tangential Flow Filtration, and Multimode Chromatography

Номер: US20190010187A1

Автор: Datta Anup K., Kapre Subhash V.

Принадлежит: Inventprise, LLC

An improved, cost effective and shortened process of purification of the capsular polysaccharide of , Group B and is disclosed. The process includes a cocktail of enzyme treatment, tangential flow filtration, and multimodal chromatography purification. For Gram-negative bacteria, endotoxin removal process involves endotrap HD resin. This shortened process achieved the purity required by WHO/EP/BP for the use in human vaccine preparation, with simple steps and higher yield as compared to conventional processes. The steps of the process avoid use of organic solvents such as, for example, alcohol, phenol, and ultracentrifugation that are otherwise expensive and time consuming to perform, and/or health hazards for commercial use. This process disclosed is also simple, efficient, non-toxic, easy to scale-up, and environmentally friendly. 1. A process of purifying polysaccharide comprising:providing a fermentation harvest of Gram-positive and/or Gram-negative bacteria;clarifying the fermentation harvest with deoxycholate;concentrating the clarified polysaccharide by a first diafiltration;treating the first diafiltered polysaccharide with an enzyme to digest impurities;precipitating the enzyme with acetic acid;concentrating the polysaccharide by a second diafiltration;passing the second diafiltered polysaccharide through multimodal chromatographic resin and/or endotoxin removal resin; andcollecting the purified polysaccharide.2. The process of claim 1 , wherein the polysaccharide comprises bacterial cell surface polysaccharide.3. The process of claim 2 , wherein the bacterial cell surface polysaccharide comprises capsular polysaccharide and/or exopolysaccharide.4S. pneumoniaeStreptococcus, H. influenzae, S. typhimuriumN. meningitis.. The process of claim 1 , wherein the fermentation harvest comprises claim 1 , Group B and/or5. The process of claim 1 , wherein clarifying is followed by pH adjustment to about pH 3.5-5.0.6. The process of claim 5 , wherein the pH adjustment ...

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Номер записи: 38

15-01-2015 дата публикации

ELECTROCHEMICAL SYNTHESIS OF NITRO-CHITOSAN

Номер: US20150014180A1

Автор: Halada Gary P., Jha Prashant Kumar

Принадлежит:

The present disclosure provides methods for producing chitosan derivatives and the derivatives formed by these methods. The processes of the present disclosure utilize electrochemical methods to functionalize and/or modify amine and/or hydroxyl groups present on chitosan, to form new derivatives. In embodiments, a nitro-chitosan derivative may be prepared. The altered cationic affinity of these derivatives make them excellent candidates for environmental applications, including water and waste treatments, and fertilizers. 1. A process comprising:contacting chitosan with a solvent to form a chitosan solution;adding to the chitosan solution an acid selected from the group consisting of hydrochloric acid, hypochlorous acid, organic acids, and combinations thereof, to reduce the pH of the chitosan solution to a pH from about 1 to about 7;applying a negative potential of from about −1 volt to about −5 volts to the chitosan solution by the introduction of a cathode and anode into the chitosan solution;forming a hydrogel comprising a nitro-chitosan derivative on the cathode; andrecovering the nitro-chitosan derivative from the cathode.2. The process of claim 1 , wherein the solvent is selected from the group consisting of water claim 1 , alcohols claim 1 , or other polar solvents and combinations thereof claim 1 , and wherein the chitosan is present in an amount from about 1% by weight to about 50% by weight of the chitosan solution.3. The process of claim 1 , wherein the chitosan solution is formed with heating from about 10° C. to about 90° C.4. The process of claim 1 , wherein the chitosan solution is formed with mixing at a rate of from about 1 revolution per minute to about 1000 revolutions per minute.5. The process of claim 1 , wherein the chitosan solution is formed over a period of time from about 1 minute to about 48 hours.6. The process of claim 1 , further comprising exposing the hydrogel on the cathode to a source of radiation selected from the group consisting ...

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Номер записи: 39

03-02-2022 дата публикации

Polysaccharide Derivatives and Compositions Comprising Same

Номер: US20220033531A1

Автор: ADELMAN DOUGLAS J., Lenges Christian Peter

Принадлежит:

The disclosure relates to polysaccharide derivatives comprising a polysaccharide substituted with at least one carbamate group, wherein the polysaccharide comprises poly alpha-1,3-glucan, poly alpha-1,3-1,6-glucan, or a mixture thereof, and the polysaccharide derivative has a degree of substitution of about 0.001 to about 3 with the carbamate group. The disclosure further relates to compositions comprising the polysaccharide derivative, and to a fiber, film, coating, or article comprising the polysaccharide derivative. 1. A polysaccharide derivative comprising:a polysaccharide substituted with at least one carbamate group;wherein the polysaccharide comprises poly alpha-1,3-glucan, poly alpha-1,3-1,6-glucan, or a mixture thereof; andthe polysaccharide derivative has a degree of substitution of about 0.001 to about 3.2. The polysaccharide derivative of claim 1 , wherein the polysaccharide comprises poly alpha-1 claim 1 ,3-glucan claim 1 , and the poly alpha-1 claim 1 ,3-glucan comprises a backbone of glucose monomer units wherein greater than or equal to 50% of the glucose monomer units are linked via alpha-1 claim 1 ,3-glycosidic linkages.3. The polysaccharide derivative of claim 1 , wherein the polysaccharide comprises alpha-1 claim 1 ,3-glucan claim 1 , and the poly alpha-1 claim 1 ,3-glucan comprises a backbone of glucose monomer units wherein greater than or equal to 90% of the glucose monomer units are linked via alpha-1 claim 1 ,3-glycosidic linkages.4. Them polysaccharide derivative of claim 1 , wherein the polysaccharide comprises poly alpha-1 claim 1 ,3-1 claim 1 ,6-glucan claim 1 , wherein (i) at least 30% of the glycosidic linkages of the poly alpha-1 claim 1 ,3-1 claim 1 ,6-glucan are alpha-1 claim 1 ,3 linkages claim 1 , (ii) at least 30% of the glycosidic linkages of the poly alpha-1 claim 1 ,3-1 claim 1 ,6-glucan are alpha-1 claim 1 ,6 linkages claim 1 , (iii) the poly alpha-1 claim 1 ,3-1 claim 1 ,6-glucan has a weight average degree of polymerization ...

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Номер записи: 40

18-01-2018 дата публикации

METHODS AND COMPOSITIONS FOR MAINTAINING THE CONFORMATION AND STRUCTURAL INTEGRITY OF BIOMOLECULES

Номер: US20180015135A1

Автор: Crow Brent B., Griffin Nickolas B., Hubbard Paul A., Nelson Kevin D., Patel Alpeshkumar P., Seifert Jennifer, Sood Paul R.

Принадлежит:

A composition includes a target pharmaceutical or biological agent, a solution containing the target pharmaceutical or biological agent, and substrate that is soluble in the solution. The substrate is capable of being solidified via a solidification process and the solidification process causes the substrate to become physically or chemically cross-linked, vitrified, or crystallized. As a result of the solidification process, particles are formed. The target pharmaceutical or biological agent within the solution retains proper conformation to ultimately produce a desired effect. 1. A composition comprising:a solution containing a target pharmaceutical or biological agent;a substrate that is soluble in the solution, wherein some components of said substrate are capable of being solidified via a solidification process, wherein the solidification process causes the substrate to become physically or chemically cross-linked, vitrified, or crystallized, and wherein particles are formed after or as a result of the solidification process, wherein the target pharmaceutical or biological agent within the resulting particles retains proper conformation to ultimately produce a desired effect, and wherein the resulting particles are poorly swellable in a solvent from which protection is desired.2. The composition of claim 1 , wherein the substrate is a protein claim 1 , carbohydrate claim 1 , or synthetically derived molecule.3. The composition of claim 2 , wherein the protein is from the families of gelatins claim 2 , collagens claim 2 , or fibrins.4. The composition of claim 2 , wherein the carbohydrate may be from the families of monosaccharides claim 2 , disaccharides claim 2 , oligosaccharides claim 2 , and polysaccharides claim 2 , with illustrative examples to include sucrose claim 2 , trehalose claim 2 , maltose claim 2 , dextran claim 2 , starch claim 2 , alginates claim 2 , xanthan claim 2 , galactomanin claim 2 , agar claim 2 , or agarose.5. The composition of claim 2 ...

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Номер записи: 41

21-01-2016 дата публикации

ORGANIC ACID CARBOHYDRATE BINDERS AND MATERIALS MADE THEREWITH

Номер: US20160017063A1

Автор: MUELLER Gert R.

Принадлежит:

A binder comprising a polymeric binder comprising the products of a carbohydrate reactant and organic acid is disclosed. The binder is useful for consolidating loosely assembled matter, such as fibers. Fibrous products comprising fibers in contact with a carbohydrate reactant and an organic acid are also disclosed. The binder composition may be cured to yield a fibrous product comprising fibers bound by a cross-linked polymer. Further disclosed are methods for binding fibers with the carbohydrate based binder using an organic acid.

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Номер записи: 42

18-01-2018 дата публикации

CHITIN, HYDROLYSATE AND METHOD FOR THE PRODUCTION OF ONE OR MORE DESIRED PRODUCTS FROM INSECTS BY MEANS OF ENZYMATIC HYDROLYSIS

Номер: US20180016357A1

Автор: Berezina Nathalie, Berro Fabrice, Hubert Antoine, Laurent Sophie, Le Roux Karine, Levon Jean-Gabriel, Sanchez Lorena, Socolsky Cecilia

Принадлежит: YNSECT

The invention relates to chitin, a hydrolysate and a method for the production of at least one desired product from insects. More specifically, the invention relates to a method for the production of at least one desired product from insects, comprising an insect cuticle pressing step, followed by the enzymatic hydrolysis of the insect cuticles using a proteolytic enzyme. 1. Chitin comprising an amino acid content less than or equal to 45% by weight based on the total weight of dry matter , an ash content less than or equal to 3.5% by weight based on the total weight of dry matter and a purity by difference greater than or equal to 45%.2. Hydrolysate comprising at least 40% by weight proteins based on the total weight of dry matter , at a maximum 10% by weight ash based on the total weight of dry matter , and a water-soluble protein content larger than 12 ,400 g/mol less than 50%.3. Method for the production of at least one product of interest from insects , comprising the following steps:(i) grinding the insect cuticles,(ii) pressing the insect cuticles, and then(iii) enzymatic hydrolysis of the insect cuticles with a proteolytic enzyme.4. Method according to claim 3 , comprising a step of killing the insects prior to the grinding step.5. Method according to or claim 3 , further comprising a step of treatment of the insect cuticles with an oxidizing agent prior to enzymatic hydrolysis.6. Method according to any one of to claim 3 , in which the insect or insects is/are selected from the group constituted by the Coleoptera claim 3 , the Lepidoptera claim 3 , the Orthoptera and the Diptera.7. Method according to any one of to claim 3 , in which the protease is selected from the group constituted by aminopeptidases claim 3 , metallocarboxypeptidases claim 3 , serine endopeptidases claim 3 , cysteine endopeptidases claim 3 , aspartic endopeptidases claim 3 , metalloendopeptidases.8. Method according to any one of to claim 3 , in which the product of interest is chitin ...

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Номер записи: 43

18-01-2018 дата публикации

PROCESS FOR OBTAINING INULIN FROM ROOTS OF THE CARDOON PLANT

Номер: US20180016358A1

Автор: Bastioli Catia, Capuzzi Luigi, Carotenuto Giuseppina

Принадлежит:

This invention relates to a new process for obtaining inulin from roots of cardoon plants, that is those belonging to the Cardueae tribe. 1. A process for obtaining inulin starting from roots of plants belonging to the Cardueae tribe , comprising the steps of:a) Comminuting said roots to obtain cossettes having maximum thickness of 1 cm;b) Leaching by means of at least one cavitation treatment inulin from said cossettes in presence of an aqueous solution;c) separating from step b) an aqueous phase, containing inulin, and a solid phase, containing exhausted cossettes;d) purifying said aqueous phase containing inulin;wherein said cossettes are fed to said leaching step) at ambient temperature.2. The process according to claim 1 , comprising before step a) one or more pretreatment steps of said roots claim 1 , selected from the group consisting of:(i) topping;(ii) cleaning and screening;(iii) washing;(iv) drying.3. The process according to claim 1 , in which said step a) is performed at a temperature equal to or less than 90° C.4. The process according to claim 1 , in which said step b) is performed at a temperature equal to or less than 90° C.5. The process according to claim 1 , in which said step b) is performed in a hydrodynamic cavitator.6. The process according to claim 1 , in which said step b) is performed at a pressure in the range of 0.1-3.5 MPa.7. The process according to claim 1 , in which said step b) is carried out for a time of less than 60 minutes.8. The process according to claim 1 , in which said step b) is performed with an aqueous solution having pH in the range of 5-9.9. The process according to claim 1 , in which said step b) is performed using up to 15 parts per weight of aqueous solution per part of cossettes.10. The process according to claim 1 , in which said step c) is performed by means of one or more operations selected from the group consisting of filtration claim 1 , centrifugation claim 1 , sedimentation claim 1 , decantation claim 1 , ...

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Номер записи: 44

17-01-2019 дата публикации

Systems and methods for dewatering a slurry that includes lignocellulosic biomass and liquid

Номер: US20190016829A1

Автор: David Charles Carlson

Принадлежит: Poet Research Inc

The present disclosure relates to a dewatering systems, and related methods, that are adapted to convey lignocellulosic biomass to separate at least a portion of the water from a lignocellulosic biomass slurry and accumulate the dewatered lignocellulosic biomass. The dewatering system also includes a headspace occupied by a gas that is at a pressure that facilitates transferring the accumulated biomas into a pretreatment reactor having a pressurized headspace. Such a dewatering system can prevent undue mixing and backflow of gas (e.g., steam) from the pretreatment reactor.

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Номер записи: 45

16-01-2020 дата публикации

GELLAN GUM WITH DOUBLE SETTING TEMPERATURES AND THE PREPARATION METHOD AND USE THEREOF

Номер: US20200017609A1

Автор: Liu Guojun, Yuan Chienkuo, Zhao Jie

Принадлежит:

A gellan gum product having double setting temperatures, its preparation method and use thereof are provided. The obtained product has low setting temperatures, and the provided preparation method has the advantages of simple pretreatment of fermentation broth, shortened production cycle and direct extraction of such gellan gum product from the fermentation broth 1. A gellan gum product , characterized in that the gellan gum product has double setting temperatures.2. The gellan gum product according to claim 1 , wherein the double setting temperatures are: the first setting temperature in the range of 30° C. to 40° C. claim 1 , and the second setting temperature in the range of 40° C. to 65° C.3. A method for preparing the gellan gum product according to claim 1 , wherein the method comprises the following steps:(1) Pretreatment of gellan gum fermentation broth: adding an alkali metal salt into gellan gum fermentation broth, and stirring to obtain pretreated fermentation broth;(2) Deacylation of fermentation broth: adjusting pH of the pretreated fermentation broth with an alkali, stirring, adjusting pH back with an acid, and stirring to obtain deacylated fermentation broth;(3) Removal of metal ions from fermentation broth: adding a metal ion chelating agent into the deacylated fermentation broth, and stirring to obtain fermentation broth with metal ions removed;(4) pH adjustment back of fermentation broth: adjusting back pH of the fermentation broth with metal ions removed with an acid, and stirring to obtain fermentation broth with pH adjusted back;(5) Heating of fermentation broth: heating the fermentation broth with pH adjusted back of step (4), and keeping the temperature to obtain hot gel solution; and(6) Alcohol precipitation, separation, drying and milling of fermentation broth: precipitating the hot gel solution of step (5) by using alcohol, separating, drying, and milling to obtain the gellan gum product.4. The method according to claim 3 , wherein said ...

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Номер записи: 46

21-01-2021 дата публикации

COMPOSITION AND METHODS FOR PREPARING HEMICELLULOSE-RICH EXTRACT FROM SPEND COFFEE GROUND

Номер: US20210017299A1

Автор: CHEE Celia, LEE Peter, LU Yingshuang, MUCHENA John Kailemia, Pan Li, POTINENI Rajesh

Принадлежит: Kerry Luxembourg S.à.r.l.

Methods of preparing a hemicellulose-product and a holocellulose-product from a carbohydrate-rich material, including spend coffee grounds, are described. Hemicellulose-products and holocellulose-products produced according to these methods are also described. 1. A method of producing a hemicellulose product , the method comprising:combining an aqueous slurry of a carbohydrate-rich material with an alkaline hydrogen peroxide solution;separating a solid fraction from a liquid fraction of the alkaline hydrogen peroxide treated slurry;adjusting the pH of the liquid fraction to a pH between about 4.0-6.0 using a pH adjusting agent;combining an alcohol solution with the pH adjusted liquid fraction so as to precipitate a hemicellulose product; anddrying the hemicellulose product.2. The method of claim 1 , wherein the carbohydrate-rich material is spend coffee grounds.3. The method of claim 2 , further comprising a step of defatting the aqueous slurry by adding an organic solvent to the aqueous slurry prior to the step of adding an alkaline hydrogen peroxide solution.4. The method of claim 2 , further comprising a step of concentrating the liquid fraction.5. The method of claim 4 , wherein the concentrating step is carried out using a vacuum rotavapor.6. The method of claim 4 , wherein the concentrating step is carried out by membrane filtration.7. The method of claim 2 , further comprising a step of purifying the dried hemicellulose product.8. The method of claim 7 , wherein the step of purifying the dried hemicellulose product comprises steps of dissolving the dried hemicellulose product in an aqueous solution and purifying the dissolved hemicellulose product by membrane filtration.9. The method of claim 7 , wherein the step of purifying the dried hemicellulose product comprises steps of dissolving the dried hemicellulose product in an aqueous solution and purifying the dissolved hemicellulose product by column chromatography.10. The method of claim 4 , further ...

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Номер записи: 47

21-01-2021 дата публикации

METHOD FOR SEPARATION OF PROTEIN AND OTHER IMPURITIES FROM MICROBIAL CAPSULAR POLYSACCHARIDES

Номер: US20210017300A1

Автор: Charan Kantam, Datla Mahima, Kandimalla Vivek Babu, Mantena Narender Dev, Matur Ramesh Venkat, Reddy Muthyala Venkateswara

Принадлежит:

The invention relates to a method for the removal of protein and other impurities from microbial capsular polysaccharides. More particularly, the present invention relates to isolation of microbial capsular polysaccharides in pure form after removal of protein and other impurities. 117-. (canceled)18Neisseria meningitidis, Streptococcus pneumoniae, Haemophilus influenzaeSalmonella typhi. An immunogenic composition comprising a capsular polysaccharide conjugated to a carrier protein selected from the group of carrier proteins consisting of diphtheria toxoid , tetanus toxoid , and CRM , wherein the capsular polysaccharide is isolated from bacteria selected from the group consisting of type b , and by a process comprising the steps of—{'sub': '2', 'exposing or contacting a solution of lysed cells comprising the capsular polysaccharide, proteins, nucleic acids, cell wall components and other impurities with silicone dioxide (SiO); and'}{'sub': '2', 'separating the capsular polysaccharide from the SiOby filtration or by centrifugation and without using chromatography to isolate the capsular polysaccharide in substantially pure form.'}19Streptococcus pneumonia. The immunogenic composition of claim 18 , wherein the bacteria is and comprises one or more serotypes selected from the group comprising of 1 claim 18 , 2 claim 18 , 3 claim 18 , 4 claim 18 , 5 claim 18 , 6A claim 18 , 6B claim 18 , 7F claim 18 , 8 claim 18 , 9N claim 18 , 9V claim 18 , 10A claim 18 , 11A claim 18 , 12F claim 18 , 14 claim 18 , 15B claim 18 , 17F claim 18 , 18C claim 18 , 19F claim 18 , 19A claim 18 , 20 claim 18 , 22F claim 18 , 23F claim 18 , and 33F.20. The immunogenic composition of claim 18 , wherein a the SiOcomprises particles each having a size in a size range from 0.01 μm to 200 μm.21. The immunogenic composition of claim 18 , wherein the solution of lysed cells is exposed or contacted with the SiOat a temperature in a temperature range from 15° C. to 60° C. for a period of time ranging ...

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Номер записи: 48

26-01-2017 дата публикации

POROUS ASYMMETRIC POLYPHENYLENE ETHER MEMBRANES AND ASSOCIATED SEPARATION MODULES AND METHODS

Номер: US20170021310A1

Автор: Bajaj Pooja, Berzinis Albin Peter, Bikel Matias, Halbfinger Rachel Elizabeth

Принадлежит:

A porous asymmetric membrane comprises a hydrophobic polymer comprising a poly(phenylene ether) or poly(phenylene ether) copolymer; and a polymer additive. A separation module can be fabricated from the porous asymmetric membrane. A method of forming the porous asymmetric membrane comprises: dissolving a hydrophobic polymer comprising a poly(phenylene ether) or poly(phenylene ether) copolymer and, a polymer additive in a water-miscible polar aprotic solvent to form a porous asymmetric membrane-forming composition; and phase-inverting the porous asymmetric membrane forming-composition in a first non-solvent composition to form the porous asymmetric membrane. The polymer additive comprises hydrophilic functional groups, copolymerized hydrophilic monomers, or blocks of hydrophilic monomer repeat units. For example, the polymer additive can comprise a hydrophilic polymer or amphiphilic polymer. The porous asymmetric membrane can be a flat membrane or hollow fiber. 1. A separation module comprising a porous asymmetric membrane , comprising a poly(phenylene ether) copolymer comprising repeat units derived from 2 ,6-dimethylphenol and repeat units derived from 2-methyl-6-phenylphenol , and having an intrinsic viscosity of 0.7 to 1.5 deciliters per gram , measured in chloroform at 25° C. and a weight average molecular weight of 100 ,000 to 500 ,000 daltons , measured by gel permeation chromatography against polystyrene standards; and an amphiphilic block copolymer; wherein the porous asymmetric membrane is in a flat sheet , hollow fiber , capillary , or tubular configuration.2. The separation module of claim 1 , wherein the porous asymmetric membrane is a flat sheet wound into a spiral.3. (canceled)4. The separation module of claim 1 , wherein the porous asymmetric membrane is a hollow fiber.5. (canceled)6. The separation module of claim 4 , wherein the separation module comprises:an enclosure configured to contain a bundle of the porous hollow fibers, the enclosure having ...

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Номер записи: 49

22-01-2015 дата публикации

PROCESS FOR SEPARATING BIOMASS COMPONENTS

Номер: US20150025229A1

Автор: Pandey Banibrata, PECHIMUTHU Sakthi Priya, SARKAR Manoj Kumar, SRIVASTAVA Suresh Chandra, SUDHAKARAN Dinakaran Samuel

Принадлежит: NAGARJUNA ENERGY PRIVATE LIMITED

The present invention provides a process and System for Separation of biomass components into individual components such as cellulose, hemicellulose and lignin. The present invention provides a process for separating lignin in its native form. The cellulose obtained by the process of the present invention is highly reactive for saccharification. 111-. (canceled)12. A process for separating biomass components namely cellulose , hemicellulose and lignin , said process comprising steps:a) contacting biomass with an alkaline agent capable of dissolving essentially lignin in said biomass under a first predetermined temperature and a first pressure to dissolve lignin;b) removing the lignin under pressure;c) reacting mild acid or water under a second predetermined temperature and a second pressure with the remaining residue of step (a) to hydrolyze hemicellulose and subsequently removing the same from biomass;d) obtaining cellulose from the remaining biomass.13. A process as claimed in claim 12 , wherein the alkaline agent is selected from the group comprising ammonia or derivatives thereof.14. A process as claimed in claim 12 , wherein the alkaline agent is contacted with the biomass at a temperature in the range of 90° to 200° C. and at a pressure in the range of 7.5-25 Bar.15. A process as claimed in claim 12 , wherein the alkaline agent is contacted with the biomass for a period in the range of 1 to 30 minutes.16. A process as claimed in claim 13 , wherein the concentration of ammonia is in the range of 10% to 30%.17. A process as claimed in claim 12 , wherein the mild acid is selected from a group comprising mineral acids having a concentration of 0.25% to 2%.18. A process as claimed in claim 12 , wherein the mild acid is reacted with the residual biomass at a temperature in the range of 120°-200° C. and at a pressure in the range of 1.5-20 Bar.19. A system for separating biomass comprising:(a) a reactor chamber for containing biomass having at least one inlet and at ...

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Номер записи: 50

10-02-2022 дата публикации

LOW-MOLECULAR-WEIGHT TREMELLA AURANTIALBA GLUCURONOXYLOMANNAN AS WELL AS PREPARATION METHOD AND APPLICATION THEREOF

Номер: US20220041762A1

Автор: LI HONG, WEI Ziyi, YUAN Qingxia, ZHAO Longyan

Принадлежит: GUANGXI UNIVERSITY OF CHINESE MEDICINE

The present disclosure provides a low-molecular-weight glucuronoxylomannan (LTAG) as well as a preparation method and an application thereof, and specifically relates to the technical field of medicine. The LTAG provided in the present disclosure has a weight-average molecular weight of 8,000-24,000 Da. In the method of preparing LTAG as provided in the present disclosure, glucuronoxylomannan is depolymerized by peroxides so as to get low-molecular-weight products, which are then exchanged into pharmaceutically acceptable salts through cation exchange resins. The resulting LTAG has a clear structure, a low viscosity and a good solubility, has a strong immune-enhancing activity, and is capable of acting on TLR4 receptor-activated macrophagocytes and promoting the production of various immune factors, so it can be used in the prevention and/or treatment of immunodeficiency-related diseases. 2Tremella aurantialbaTremella aurantialba. The low-molecular-weight glucuronoxylomannan according to claim 1 , wherein claim 1 , on the basis of molar percentage claim 1 , the compound in which Ris —OH accounts for more than 90% the total amount of the low-molecular-weight glucuronoxylomannan.3Tremella aurantialbaTremella aurantialba. The low-molecular-weight glucuronoxylomannan according to claim 1 , wherein claim 1 , in the low-molecular-weight glucuronoxylomannan claim 1 , the molar percentage of acetyl is 10%.4Tremella aurantialbaTremella aurantialba. The low-molecular-weight glucuronoxylomannan according to claim 1 , wherein claim 1 , on the basis of molar ratio claim 1 , the molar ratio of the three monosaccharide residues claim 1 , mannose claim 1 , glucuronic acid and xylose claim 1 , contained in the low-molecular-weight glucuronoxylomannan to the contained —COCHis 3:(1±0.3):(1±0.3):(0.5±0.05).5Tremella aurantialbaTremella aurantialba. The low-molecular-weight glucuronoxylomannan according to claim 4 , wherein claim 4 , on the basis of molar ratio claim 4 , the molar ratio ...

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Номер записи: 51

10-02-2022 дата публикации

AMPHIPHILIC POLYSACCHARIDE DERIVATIVES AND COMPOSITIONS COMPRISING SAME

Номер: US20220041960A1

Автор: FLITER Kristi Lynn, Huang Zhengzheng, Lu Helen, Qiu Weiming, Shah Mukesh C., Shuey Steven W., SIVIK Mark Robert

Принадлежит:

The disclosure relates to compositions comprising a polysaccharide derivative, wherein the polysaccharide derivative comprises a polysaccharide substituted with a) at least one hydrophobic group, and b) at least one hydrophilic group, wherein the polysaccharide is poly alpha-1,3-glucan, poly alpha-1,6-glucan, or poly alpha-1,3-1,6-glucan. 120-. (canceled)21. A product comprising:from about 1% to about 60% by weight of a surfactant; andfrom about 0.1% to about 10% by weight of a polysaccharide derivative, wherein the polysaccharide derivative comprises a polysaccharide substituted witha) at least one hydrophobic group; andb) at least one hydrophilic group;wherein the polysaccharide is poly alpha-1,3-glucan, poly alpha-1,6-glucan, or poly alph-1,3-1,6-glucan; wherein said product is a household product.22. The product of in the form of a liquid claim 21 , a gel claim 21 , a powder claim 21 , a hydrocolloid claim 21 , an aqueous solution claim 21 , a granule claim 21 , a tablet claim 21 , a capsule claim 21 , a single compartment sachet claim 21 , a multi-compartment sachet claim 21 , a single compartment pouch claim 21 , or a multi-compartment pouch.23. The product of claim 21 , further comprising at least one of an enzyme claim 21 , a detergent builder claim 21 , a complexing agent claim 21 , a polymer claim 21 , a soil release polymer claim 21 , a surfactancy-boosting polymer claim 21 , a bleaching agent claim 21 , a bleach activator claim 21 , a bleaching catalyst claim 21 , a fabric conditioner claim 21 , a clay claim 21 , a foam booster claim 21 , a suds suppressor claim 21 , an anti-corrosion agent claim 21 , a soil-suspending agent claim 21 , an anti-soil re-deposition agent claim 21 , a dye claim 21 , a bactericide claim 21 , a tarnish inhibitor claim 21 , an optical brightener claim 21 , a perfume claim 21 , a saturated or unsaturated fatty acid claim 21 , a dye transfer inhibiting agent claim 21 , a chelating agent claim 21 , a hueing dye claim 21 , a ...

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Номер записи: 52

29-01-2015 дата публикации

Immunomodulatory conjugates

Номер: US20150030626A1

Автор: Clement Leong, Geoffrey Alan Pietersz

Принадлежит: Ascend Biopharmaceuticals Pty Ltd

The present invention provides an immunomodulatory compound comprising a carbohydrate polymer comprising mannose, wherein the carbohydrate polymer is conjugated to at least one immune modulator. The present invention also provides for the use of this compound in immunomodulatory compositions for vaccination and gene therapy methods, together with processes for its preparation.

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Номер записи: 53

24-04-2014 дата публикации

METHOD AND A SYSTEM FOR MANUFACTURING CELLULOSIC MATERIAL

Номер: US20140114059A1

Автор: Kosonen Harri, Nuopponen Markus, Tamper Juha

Принадлежит: UPM-KYMMENE CORPORATION

A method for manufacturing cellulosic material includes: introducing cellulose fibers as cellulosic raw material to a system, conveying the cellulose fibers to an extruder comprising a mixing part and/or a refining part, dosing at least one chemical to the system before the extruder and/or in the extruder and performing a reaction between the cellulose fibers and the chemical(s) at least partly in the extruder, wherein the reaction is performed at a consistency of at least 40%, and/or dosing at least one chemical to the system before the extruder and/or in the extruder and mixing the cellulose fibers and the chemical(s) in the mixing part of the extruder, wherein the mixing step is performed at a consistency of at least 40%, and/or refining the introduced cellulose fibers at least partly in the refining part of the extruder, wherein the refining step is performed at a consistency of at least 5%. 2. The method according to claim 1 , the method comprising:dosing a first chemical before the extruder or in the mixing part of the extruder, anddosing a second chemical in the mixing part of the extruder.3. The method according to claim 2 , wherein the first chemical is sodium hydroxide and the second chemical is monochloroacetic acid or a salt thereof.4. The method according to claim 1 , wherein the chemical comprises TEMPO chemical and that the method comprises:performing a reaction between the cellulose fibers and the TEMPO-chemical in the extruder in order to manufacture TEMPO-treated cellulosic material.5. The method according to claim 1 , the method comprising:refining the cellulose fibers in a prerefiner before conveying the cellulose fibers to the extruder.6. The method according to claim 1 , wherein the extruder comprises a heating part and the method comprises:using the heating part of the extruder in order to achieve a predetermined reaction temperature.7. The method according to claim 6 , wherein the temperature in the heating part is increased by a hot steam ...

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Номер записи: 54

04-02-2016 дата публикации

MICROPROCESSING FOR PREPARING A POLYCONDENSATE

Номер: US20160032022A1

Автор: BENZINGER Walther, BRANDNER Jurgen J., KÜSTERS Christof Franz, Stengel Bruno Frederic

Принадлежит:

The present invention relates to a process for preparing polydextrose by using a microdevice. It further relates to the use of a microdevice for the polycondesation reactions. 114-. (canceled)15. An arrangement of microdevices for the following process for preparing polydextrose.a) providing glucose;b) adding an acidifying catalyst to the glucose to provide an acidic composition;c) injecting the acidic composition through a microdevice; andd) collecting polydextrose,wherein the arrangement allows a single-pass-through of the composition of step c) a re-mix of the polydextrose of step d) with the acidic composition of step c) for a multi-pass-through, or a complete multi-pass-through for the acidic composition. The present invention relates to a process for preparing polydextrose, by using microdevices.In order to continually improve physical standards of living for greater number of people, it is necessary to achieve more results with fewer resources. Therefore there is the tendency towards building and manufacturing smaller-scale products due to the desire for size efficiency. Most recently, scientists have learned that not only electronic devices, but also mechanical devices, may be miniaturized and batch-fabricated, promising the same benefits to the mechanical world as integrated circuit technology has given to the electronic world.Acid-catalysed polymerisation of saccharides is a well-known phenomenon which is described in numerous general articles, books and patents.Polydextrose is commercially available and all of these polydextrose products include a variety of residual compounds such as glucose, sorbitol, citric acid and other compounds which contribute to the taste, colour, and caloric value. Low molecular weight compounds such as 1,6-anhydroglucose and 5-hydroxymethylfurfural contribute a bitter taste and off-flavour.U.S. Pat. No. 3,766,165 discloses that polymers useful as low-calorie food ingredients can be prepared by heating dextrose or maltose, ...

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Номер записи: 55

17-02-2022 дата публикации

Efficient methods and compositions for recovery of products from organic acid pretreatment of plant materials

Номер: US20220049421A1

Автор: Feng Ling

Принадлежит: Pierson Capital Environmental Beijing Ltd

The invention is directed to compositions and processes concerning efficient downstream processing of products derived from organic acids pretreatment of plant materials.

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Номер записи: 56

04-02-2021 дата публикации

ISOLATED SAPOSHNIKOVIA DIVARICATA POLYSACCHARIDE AND USE THEREOF

Номер: US20210032374A1

Автор: Pang Li, Shen Lulu, Xu Naiyu, Xue Jie, YIN Xiang, ZHANG Zhenqing

Принадлежит:

The present application relates to the field of medicine. The present application relates to an isolated polysaccharide and use thereof in the manufacture of a medicament for treating diabetes mellitus or hyperlipoidemia. In particular, the present application relates to an isolated polysaccharide comprising L-arabinose, D-galacturonic acid, D-mannose, D-glucose and D-galactose, wherein the molar ratio of the L-arabinose: D-galacturonic acid: D-mannose: D-glucose: D-galactose is 1-15:1-10:1-10:10-40:1-15, preferably 1-5:5-10:1-5:20-25:1-5. 1Saposhnikovia divaricata. An isolated polysaccharide , comprising L-arabinose: D-galacturonic acid: D-mannose: D-glucose: D-galactose , wherein the molar ratio of the L-arabinose: D-galacturonic acid: D-mannose: D-glucose: D-galactose is 1-15:1-10:1-10:10-40:1 15.2Saposhnikovia divaricata. The isolated polysaccharide of claim 1 , wherein claim 1 ,the L-arabinose includes 1,4-linked L-arabinose and/or 1,3,4-linked L-arabinose;the D-galacturonic acid includes terminal D-galacturonic acid;the D-mannose includes 1,6-linked D-mannose;the D-glucose includes 1,4-linked D-glucose and/or 1,3,6-linked D-glucose; andthe D-galactose includes terminal D-galactose and/or 1,4-linked D-galactose.3Saposhnikovia divaricata. The isolated polysaccharide of claim 2 , wherein the molar ratio of the 1 claim 2 ,4-linked L-arabinose: 1 claim 2 ,3 claim 2 ,4-linked L-arabinose: terminal D-galacturonic acid: 1 claim 2 ,6-linked D-mannose: 1 claim 2 ,4-linked D-glucose: 1 claim 2 ,3 claim 2 ,6-linked D-glucose: terminal D-galactose: 1 claim 2 ,4-linked D-galactose is 1-5:1-10:1-10:1-10:10-30:1-10:1-5:1-10.4Saposhnikovia divaricataSaposhnikovia divaricata. The isolated polysaccharide of claim 3 , wherein the isolated polysaccharide has a molecular weight ranging from 5 ×10to 5 ×10Da.5Saposhnikovia divaricata. A method for preparing the isolated polysaccharide of claim 1 , wherein the method comprises the steps of:{'i': Saposhnikovia divaricata', ' ...

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Номер записи: 57

09-02-2017 дата публикации

MEANS AND METHODS FOR PRODUCING NEISSERIA MENINGITIDIS CAPSULAR POLYSACCHARIDES OF LOW DISPERSITY

Номер: US20170037440A1

Автор: BERGER Monika, Bethe Andrea, FIEBIG Timm, Freiberger Friedrich, Gerardy-Schahn Rita, KEYS Tim, ROMANOW Angela

Принадлежит: MEDIZINISCHE HOCHSCHULE HANNOVER

The present invention relates to in vitro methods for producing capsular polysaccharides which have a defined length. The present invention also relates to compositions comprising at least one capsule polymerase, at least one donor carbohydrate and at least one acceptor carbohydrate, wherein the ratio of donor carbohydrate to acceptor carbohydrate is a ratio from 10:1 to 400:1. Moreover, the present invention provides truncated versions of the capsule polymerases of serogroups A and X. Also provided herein are pharmaceuticals, in particular vaccines, comprising the synthetic capsular polysaccharides of which have a defined length. Furthermore, the invention provides for methods for the production of said vaccines. 2Neisseria meningitidis. In vitro method for producing capsular polysaccharides which have a defined length , said method comprising the steps:(a) incubating at least one capsule polymerase with at least one donor carbohydrate and at least one acceptor carbohydrate; wherein the incubation time ranges from 3 to 45 minutes; and(b) isolating the resulting capsular polysaccharides,{'i': 'Neisseria meningitidis', 'wherein the capsule polymerase is a truncated version of the capsule polymerase of serogroup X.'}3Neisseria meningitidis. In vitro method for producing capsular polysaccharides which have a defined length , said method comprising the steps: (i) the ratio of donor carbohydrate to acceptor carbohydrate is a ratio from 10:1 to 10000:1, and', '(ii) the incubation time ranges from 3 to 45 minutes; and, '(a) incubating at least one capsule polymerase with at least one donor carbohydrate and at least one acceptor carbohydrate; wherein'}(b) isolating the resulting capsular polysaccharides,{'i': 'Neisseria meningitidis', 'wherein the capsule polymerase is a truncated version of the capsule polymerase of serogroup X.'}4. A composition comprising:(i) at least one capsule polymerase;(ii) at least one donor carbohydrate; and(iii) at least one acceptor carbohydrate ...

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Номер записи: 58

08-02-2018 дата публикации

METHOD FOR ISOLATION OF POLYSACCHARIDES

Номер: US20180037672A1

Автор: Bavington Charles Daniel, Moss Claire

Принадлежит:

A process for the isolation of modified polysaccharide product from potato, in particular a polysaccharide product that can be used as an immunomodulatory is described. More particularly, the extraction and modification of a rhamnogalacturonan I(RG-1) fragment product from a potato using a defined process, and the use of this product to provide immunomodulatory activity to a subject. 1. A process for the preparation of a fragment of RG-1 , wherein the process comprises the steps:providing RG-1 obtained from the enzymatic extraction of potatopreparing a fragment of said RG-1, the fragment having an average molecular weight in the range 5 kDa to 30 kDa, by selective depolymerisation of said RG-1 to provide the fragment wherein the fragment has a monosaccharide composition comprisingArabinose 7-13%,Rhamnose 5-10%,Xylose 0-1%,Galacturonic acid 20-40% andGalactose 35-60%.3. A fragment obtained from a process comprisingproviding RG-1 obtained from the enzymatic extraction of potatopreparing a fragment of said RG-1, the fragment having an average molecular weight in the range 5 kDa to 30 kDa, by selective depolymerisation of said RG-1 to provide the fragment wherein the fragment has a monosaccharide composition comprisingArabinose 7-13%,Rhamnose 5-10%,Galacturonic acid 20-40% andGalactose 35-60%.4. A method of treatment of at least one of inflammatory disease claim 2 , arthritis claim 2 , respiratory inflammation claim 2 , inflammatory bowel disease claim 2 , and psoriasis in a subject in need thereof claim 2 , said method comprising administering the fragment according to to said subject.5. A method of treatment of at least one of inflammatory disease claim 3 , arthritis claim 3 , respiratory inflammation claim 3 , inflammatory bowel disease claim 3 , and psoriasis in a subject in need thereof claim 3 , said method comprising administering the fragment according to to said subject.6. A neutraceutical composition comprising the fragment according to .7. A cosmeceutical ...

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Номер записи: 59

12-02-2015 дата публикации

Carbon nanotube - polysaccharide composite

Номер: US20150041730A1

Автор: Jorma Virtanen, Pasi Moilanen, Veijo Kangas

Принадлежит: Individual

The present invention provides methods for the fabrication CNT dispersions using polysaccharides, especially hemicelluloses, and most advantageously xylan. The present invention also provides methods to isolate, and purify hemicelluloses from plant materials. The present invention provides methods and compositions for the coating of solid surfaces using CNT dispersions. One currently preferred method coating of a surface is electrospraying the CNT dispersion. The present invention provides electrically conducting materials that can replace conducting plastics, graphite, and even some metals as electrical conductors. In one embodiment the present materials can be used as stealth coatings. In another embodiment the present materials can provide shield against high frequency electromagnetic radiation, while being permeable to low frequency magnetic field. In one specific application the dispersion fabricated from double walled carbon nanotubes (DWNTs), and xylan can be used to fabricate transparent electrically conducting films. In one embodiment of the present invention the surface films will be cross-linked, and these films can be used in multiple applications including supercapacitors.

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Номер записи: 60

07-02-2019 дата публикации

HIGHLY BRANCHED ALPHA-D-GLUCANS

Номер: US20190038753A1

Автор: Yao Yuan, Zhang Jingmin Joanne

Принадлежит: PURDUE RESEARCH FOUNDATION

Compositions including a highly branched alpha-D-glucan or modified forms thereof and a solute compound are described herein. The compositions can provide increased water solubility and/or increased rate of dissolution for the solute compound. The compositions can also provide increased stability for the solute compound. Methods for preparing and using compositions including a solute compound and a highly branched alpha-D-glucan are also described. 1. A modified highly branched alpha-D-glucan comprising at least a chemical group selected from acetate , phosphate , octenyl succinate , succinate , hydroxypropyl , hydroxyethyl , cationic groups , carboxymethyl , polyethylene glycol (PEG , or polyethylene oxide) , polypropylene glycol (or polypropylene oxide) , or the combination thereof.2. A modified highly branched alpha-D-glucan comprising at least one group selected from antibody , antigen , aptamer , protein , peptide , amino acid , cyclodextrin , saccharide , lipid , nucleic acid and nucleotide , dendrimer , enzyme , fluorescent group or dye , magnetic group , metal ion , metal nanoparticle , quantum dot , polymer and block co-polymer , radioactive group , or a combination thereof.3. The modified highly branched alpha-D-glucan according to is prepared by at least one treatment selected from acid hydrolysis claim 1 , oxidation claim 1 , pyrodextrinization claim 1 , enzymatic treatment using alpha-amylase claim 1 , beta-amylase claim 1 , debranching enzymes claim 1 , transglucosidase claim 1 , amyloglucosidase claim 1 , and/or protease claim 1 , bleaching claim 1 , shear force treatment claim 1 , extrusion claim 1 , kneading claim 1 , homogenization claim 1 , hydrothermal processing claim 1 , dry heating claim 1 , microwave treatment claim 1 , radiation claim 1 , or a combination thereof.4. A modified phytoglycogen or glycogen material containing at least a chemical group of octenyl succinate (S) claim 1 , polyethylene glycol (PEG) claim 1 , polyethylene oxide (PEO) ...

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Номер записи: 61

24-02-2022 дата публикации

METHOD FOR JOINT RECOVERY OF PECTIN FROM CITRUS PEELS AND ALKALINE/ACIDIC PROCESSING WATER FROM CITRUS CANNING

Номер: US20220056159A1

Автор: Chen Jianle, CHEN Shiguo, Cheng Huan, Liu Donghong, Ye Xingqian

Принадлежит:

Disclosed is a method for joint recovery of pectin from citrus peels and alkaline/acidic processing water from citrus canning, including the following steps: adding fresh citrus fruit peels to alkali/acid processing water from citrus fruit canning for extracting; filtering the resulting mixture, adding to the resulting filtrate 95% ethanol 1 to 3 times the volume of the filtrate, and then adjusting the pH to a range of 3.5 to 7, followed by standing for 10 minutes to 4 hours; filtering the product after standing, washing the resulting precipitate with 50% to 70% ethanol, and drying and crushing, thereby obtaining pectin. The method makes full use of waste resources from citrus fruit canning, solves the problem of pollution by processing discharge water, and has the advantages of saving the preparation cost of pectin from citrus peels, and improving the solubility of recovered pectin. 19.-. (canceled)10. A method for joint recovery of pectin from citrus peels and alkaline/acidic processing water from citrus canning , comprising the following steps:step 1, carrying out extraction:using a method I when involved with alkali processing water from citrus fruit canning, and using a method II when involved with acid processing water from citrus fruit canning,wherein the method I for alkali processing water from citrus fruit canning is as follows:mixing fresh citrus fruit peels with alkali processing water from citrus segment membrane removal and stirring at 10 to 40° C. for 5 to 30 minutes;the method II for acid processing water from citrus fruit canning is as follows:mixing fresh citrus fruit peels with acid processing water from citrus segment membrane removal and stirring at 70 to 95° C. for 60 to 100 minutes;step 2, filtering the mixture from step 1, adding to a resulting filtrate 95 vol % ethanol 1 to 3 times the volume of the filtrate, and adjusting pH to a range of 3.5 to 7, followed by standing for 10 minutes to 4 hours; andstep 3, filtering the product from ...

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Номер записи: 62

24-02-2022 дата публикации

AMPHIPHILIC POLYSACCHARIDE DERIVATIVES AND COMPOSITIONS COMPRISING SAME

Номер: US20220056375A1

Автор: FLITER Kristi Lynn, Huang Zhengzheng, Lu Helen S.M., Qiu Weiming, Shah Mukesh C., Shuey Steven W., SIVIK Mark Robert

Принадлежит:

The disclosure relates to compositions comprising a polysaccharide derivative, wherein the polysaccharide derivative comprises a polysaccharide substituted with a) at least one hydrophobic group, and b) at least one hydrophilic group, wherein the polysaccharide is poly alpha-1,3-glucan, poly alpha-1,6-glucan, or poly alpha-1,3-1,6-glucan. 133-. (canceled)34. A composition comprising a polysaccharide derivative ,wherein the polysaccharide derivative comprises a polysaccharide substituted with at least one hydrophilic group that comprises sulfate,wherein the polysaccharide is poly alpha-1,3-glucan that comprises a backbone of glucose monomer units of which greater than or equal to 50% are linked via alpha-1,3-glycosidic linkages,and wherein the composition is an industrial product, household product, or personal care product.35. The composition of claim 34 , wherein greater than or equal to 90% of the glucose monomer units are linked via alpha-1 claim 34 ,3-glycosidic linkages.36. The composition of claim 34 , wherein the polysaccharide is further substituted with at least one hydrophobic group.37. The composition of claim 36 , wherein the hydrophobic group comprises a Cto Caryl claim 36 , benzyl claim 36 , C-Caryl sulfonyl claim 36 , or p-toluenesulfonyl group.38. The composition of claim 34 , wherein the polysaccharide has a degree of polymerization of about 5 to about 1400.39. The composition of claim 34 , wherein the polysaccharide derivative has a degree of substitution of about 0.001 to about 3.0.40. The composition of claim 34 , wherein the composition is in the form of a liquid claim 34 , gel claim 34 , powder claim 34 , hydrocolloid claim 34 , aqueous solution claim 34 , granule claim 34 , tablet claim 34 , capsule claim 34 , single compartment sachet claim 34 , multi-compartment sachet claim 34 , single compartment pouch claim 34 , or multi-compartment pouch.41. The composition of claim 34 , wherein the composition further comprises at least one of a ...

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Номер записи: 63

07-02-2019 дата публикации

GLUCOSYLTRANSFERASE ENZYMES FOR PRODUCTION OF GLUCAN POLYMERS

Номер: US20190040432A1

Автор: Brun Yefim, HE HONGXIAN, PAYNE MARK S., Scholz Thomas

Принадлежит: E I DU PONT DE NEMOURS AND COMPANY

Reaction solutions are disclosed herein comprising water, sucrose and a glucosyltransferase enzyme that synthesizes poly alpha-1,3-glucan. The glucosyltransferase enzyme can synthesize insoluble glucan polymer having at least 50% alpha-1,3 glycosidic linkages and a number average degree of polymerization of at least 100. Further disclosed are methods of using such glucosyltransferase enzymes to produce insoluble poly alpha-1,3-glucan. 114-. (canceled)15. A method for producing insoluble poly alpha-1 ,3-glucan comprising:a) contacting at least water, sucrose, and an isolated glucosyltransferase enzyme comprising an amino acid sequence that is at least 90% identical to SEQ ID NO:4, whereby insoluble poly alpha-1,3-glucan having at least 90% alpha-1,3 glycosidic linkages is produced; andb) isolating the insoluble poly alpha-1,3-glucan produced in step (a).16. The method of claim 15 , wherein said insoluble poly alpha-1 claim 15 ,3-glucan has a number average degree of polymerization of at least 100.17. The method of claim 15 , wherein said insoluble poly alpha-1 claim 15 ,3-glucan has at least 95% alpha-1 claim 15 ,3 glycosidic linkages.18. The method of claim 17 , wherein said insoluble poly alpha-1 claim 17 ,3-glucan has at least 97% alpha-1 claim 17 ,3 glycosidic linkages.19. The method of claim 18 , wherein said insoluble poly alpha-1 claim 18 ,3-glucan has at least 99% alpha-1 claim 18 ,3 glycosidic linkages.20. The method of claim 19 , wherein said insoluble poly alpha-1 claim 19 ,3-glucan has about 100% alpha-1 claim 19 ,3 glycosidic linkages.21. The method of claim 15 , wherein step (a) further comprises contacting a primer with the water claim 15 , sucrose claim 15 , and glucosyltransferase enzyme.22. The method of claim 21 , wherein the primer comprises dextran.23. The method of claim 15 , wherein said glucosyltransferase enzyme comprises an amino acid sequence that is at least 93% identical to SEQ ID NO:4.24. The method of claim 15 , wherein said ...

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Номер записи: 64

18-02-2016 дата публикации

Exopolysaccharide for the treatment and/or care of the skin, mucous membranes and/or nails

Номер: US20160045423A1

Автор: Albert SOLEY ASTALS, Anthony Courtois, Bertrand THOLLAS, Raquel DELGADO GONZÁLEZ

Принадлежит: Lipotec SA, Polymaris Biotechnology SAS, Polynaris Biotechnology

Exopolysaccharide of a bacterial strain for use in treatment and/or care of the skin, mucous membranes, hair and/or nails, as well as its cosmetic and/or dermopharmaceutical compositions. In particular, for the aging of skin and in particular for the treatment and/or prevention of wrinkles.

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Номер записи: 65

06-02-2020 дата публикации

WOOD PROCESSING METHOD

Номер: US20200040273A1

Автор: GRONN Arne Johannes

Принадлежит: GLOMMEN TECHNOLOGY AS

The invention provides a method for generating a solid wood-based material and a hemicellulose-derived material from a wood raw material. The method includes treating the wood raw material under aqueous conditions at elevated temperature and pressure to generate a hemicellulose-containing fluid component and a solid component; separating the fluid component from the solid component; processing at least a part of the solid component into a solid wood-based material; and processing the liquid component into a hemicellulose-derived material. The invention also provides for a wood-derived fuel with a low ash content. 2. A method as claimed in claim 1 , further comprising a step of iv) processing said liquid component into a hemicellulose-derived material.3. A method as claimed in claim 1 , wherein said biomass is a non-wood lignocellulosic material claim 1 , such as straw claim 1 , bagasse claim 1 , stover claim 1 , grass or any mixtures thereof claim 1 , preferably straw claim 1 , bagasse claim 1 , or any mixtures thereof.4. A method as claimed in claim 1 , wherein said solid biomass-derived material has an ash deformation temperature of at least 1050° C. claim 1 , preferably at least 1100° C. claim 1 , preferably at least 1200° C. claim 1 , more preferably at least 1300° C.5. A method as claimed in claim 1 , wherein said solid biomass-derived material has a chlorine content of 0.2 wt % or less claim 1 , preferably 0.1 wt % or less claim 1 , more preferably 0.08 wt % or less.6. A method as claimed in claim 1 , wherein the process is carried out in the absence of additives for increasing the ash melting temperature claim 1 , such as mineral agents claim 1 , e.g. calcium carbonate claim 1 , lime or limestone.7. The method of wherein step i) comprises steam treatment claim 1 , or steam explosion claim 1 , of the biomass raw material whereby to generate a steam treated biomass material and optionally washing said treated biomass material with an aqueous material such as ...

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Номер записи: 66

16-02-2017 дата публикации

ASYMMETRIC POLY(PHENYLENE ETHER) CO-POLYMER MEMBRANE, SEPARATION MODULE THEREOF; AND METHODS OF MAKING

Номер: US20170043297A1

Автор: Bajaj Pooja, Berzinis Albin Peter, Bikel Matias, Halbfinger Rachel Elizabeth

Принадлежит:

A porous membrane made from a poly(phenylene ether) copolymer has at least one of: a molecular weight cut off of less than 40 kilodaltons or a surface pore size of 0.001 to 0.1 micrometers. The porous membrane is made by dissolving the poly(phenylene ether) copolymer in a water-miscible polar aprotic solvent to form a porous membrane-forming composition; and phase-inverting the porous asymmetric membrane forming-composition in a first non-solvent composition to form the porous mem-brane. The porous membrane can be in the form of a sheet or a hollow fiber, and can be fabricated into separation modules. 1. A separation module comprising a porous membrane , wherein the porous membrane comprises a poly(phenylene ether) copolymer having at least one of a molecular weight cut off of less than 40 kilodaltons and a surface pore size of 0.001 to 0.1 micrometers.2. The separation module of claim 1 , wherein the porous membrane is a porous flat sheet.3. The separation module of claim 2 , wherein the porous flat sheet is wound into a spiral.4. The separation module of claim 1 , wherein the porous membrane is a porous hollow fiber.5. The separation module of claim 1 , wherein the porous membrane is a capillary or tubular porous membrane.6. The separation module of claim 4 , wherein the separation module comprises: an enclosure configured to contain a bundle of the porous hollow fibers claim 4 , the enclosure having an outlet configured for withdrawing a permeate fluid; a first encasement comprising a thermoset or a thermoplastic polymeric material and located at a first end of the bundle claim 4 , arranged such that the porous hollow fibers are embedded in the first encasement and communicate through the first encasement and are open on an outer face of the first encasement; a second encasement comprising a thermoset or a thermoplastic polymeric material and located at a second end of the bundle opposite the first end of the bundle claim 4 , arranged such that the porous hollow ...

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Номер записи: 67

18-02-2016 дата публикации

Polysialic acid and use for treatment of neurodegenerative and neuroinflammatory diseases

Номер: US20160046734A1

Автор: Anahita Shahraz, Harald Neumann, Jens KOPATZ, Marcus Karlstetter, Thomas LANGMANN

Принадлежит: Rheinische Friedrich Wilhelms Universitaet Bonn, Universitaet zu Koeln

The present invention relates to a branched or unbranched free or glycosidically bound polysialic acid according to general formula (1) as given as follows poly-(α(2→8 or 2→9)Neu5Ac) n (1) wherein Neu5Ac is N-acetylneuraminic acid, and n is an integer in the range from 14 to 26 and/or pharmaceutically acceptable salts thereof, a polysaccharide composition comprising the polysialic acid (1), and the use as a medicament, particularly in the therapeutic and/or prophylactic treatment of degenerative, demyelinating and inflammatory diseases of the central nervous system, and degenerative or inflammatory retinal diseases.

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Номер записи: 68

15-02-2018 дата публикации

BETA-1,3-GLUCAN DERIVATIVE AND METHOD FOR PRODUCING BETA-1,3-GLUCAN DERIVATIVE

Номер: US20180044440A1

Автор: Hayashi Masahiro, Shibakami Motonari, Tsubouchi Gen

Принадлежит:

An object of the present invention is to provide a β-1,3-glucan derivative which is a polymer having β-1,3-glucan as a main chain, and has thermoplasticity and excellent moldability, and a preparation method thereof. That is, the present invention provides a β-1,3-glucan derivative having a structure represented by General Formula (1) as a main chain. 3. The β-1 claim 1 ,3-glucan derivative according to claim 1 ,{'sup': 1', '2, 'wherein Rrepresents —COR.'}4. The β-1 claim 1 ,3-glucan derivative according to claim 1 ,{'sub': '2', 'sup': 2', '2, 'wherein the number of —CHOCORs per glucose unit is 0.1 or greater, wherein Rrepresents an aliphatic hydrocarbon group having 13 or more carbon atoms.'}5. The β-1 claim 1 ,3-glucan derivative according to claim 1 ,{'sup': '2', 'wherein a part of Rs in the β-1,3-glucan derivative is a short-chain aliphatic hydrocarbon group having 1 to 5 carbon atoms or a phenyl group.'}6. A molded body formed by molding the β-1 claim 1 ,3-glucan derivative according to .7. A manufacturing method of a molded body by molding the β-1 claim 1 ,3-glucan derivative according to .8. A preparation method of a β-1 claim 1 ,3-glucan derivative claim 1 , comprising:acylating of a part of hydroxyl groups in a polymer having glucan constituted of a β-1,3-glucoside bond as a main chain with a fatty acid.9. The preparation method of a β-1 claim 8 ,3-glucan derivative according to claim 8 ,wherein the fatty acid is a long-chain fatty acid having 13 or more carbon atoms.10. The preparation method of a β-1 claim 8 ,3-glucan derivative according to claim 8 , comprising:acylating of a part of hydroxyl groups in a polymer having glucan constituted of a β-1,3-glucoside bond as a main chain with a long-chain fatty acid having 13 or more carbon atoms, andacylating of a part of the hydroxyl groups remaining in the obtained β-1,3-glucan derivative with a short-chain fatty acid having 1 to 5 carbon atoms or benzoic acid.11. The preparation method of a β-1 claim 8 ,3- ...

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Номер записи: 69

15-02-2018 дата публикации

METHOD FOR PRODUCTION OF HEPARIN AND HEPARAN SULFATE

Номер: US20180044442A1

Автор: LORD Megan S, WHITELOCK John M

Принадлежит:

This present disclosure relates to a method of producing a proteoglycan having anticoagulant activity, comprising of and providing a cell transfected with a recombinant nucleic acid encoding a core protein having one or more glycosaminoglycan attachment sites; and incubating the cell under conditions to promote the production of a proteoglycan comprising the core protein linked to heparin and/or HS and optionally other glycosaminoglycan chains, and to heparin or heparan sulphate isolated from such proteoglycans that have significant anti-coagulant activity. The invention also relates to a recombinant proteoglycan comprising a core protein and heparin and/or heparan sulfate, to a cell capable of producing a proteoglycan comprising the core protein and heparin and/or heparan sulfate, and to a method of treating or preventing blood coagulation, or a condition associated with blood coagulation, using the recombinant proteoglycan comprising a core protein and heparin and/or heparan sulfate and/or the heparin and/or heparan sulfate isolated from such proteoglycans. 1. A method of producing a proteoglycan having anticoagulant activity , comprising:a. providing a cell comprising a recombinant nucleic acid encoding a core protein having one or more glycosaminoglycan attachment sites, wherein the core protein comprises SEQ ID NO: 1; andb. incubating the cell under conditions to promote the production of a proteoglycan comprising the core protein linked to heparin, heparan sulfate or both heparin and heparan sulfate.2. A method of producing heparin , heparan sulfate or both heparin and heparan sulfate , comprising:a. providing a cell comprising a recombinant nucleic acid encoding a core protein having one or more glycosaminoglycan attachment sites, wherein the core protein comprises SEQ ID NO: 1;b. incubating the cell under conditions to promote production of a proteoglycan comprising the core protein linked to heparin, heparan sulfate or both heparin and heparan sulfate; andc ...

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Номер записи: 70

16-02-2017 дата публикации

Glycan Sample Preparation

Номер: US20170045538A1

Автор: Guttman Andras, Lew Clarence, Szigeti Marton, Varadi Csaba

Принадлежит:

The present teachings relate to methods, systems, and kits for the preparation, purification and/or analysis of a glycan or glycoconjugate, and specifically to a magnetic bead based sample preparation protocol that can enable full automation and reduced sample preparation time relative to current methods of glycoanalysis. In some aspects, the sample preparation protocol can provide for glycoconjugate capture, glycan release, fluorescent derivatization, and glycan purification for subsequent capillary electrophoresis, liquid chromatography, or other glycoanalytical method without requiring time-consuming sample preparation steps such as centrifugation or vacuum-centrifugation. 1. A method of purifying glycans , comprising:reacting a sample containing one or more glycoconjugates with a deglycosylation reagent to release glycans from the glycoconjugates;associating the released glycans with a plurality of magnetic particles;applying a magnetic field to draw down the plurality of magnetic particles having the released glycans associated therewith;removing a supernatant from the drawn-down magnetic particles so as to remove the deglycosylation reagent and deglycosylated sample; andeluting the glycans from the magnetic particles;wherein the magnetic particles are carboxyl-coated magnetic beads.2. (canceled)3. The method of claim 1 , wherein the glycoconjugate comprises a glycoprotein or glycopeptide or antibody.4. The method of claim 3 , wherein the glycoconjugate comprises a proteoglycan claim 3 , glycosphingolipid claim 3 , chondroitin sulfate claim 3 , heparan sulfate claim 3 , hyaluronan claim 3 , glycolipid or glycoseaminoglycan claim 3 , fusion glycoprotein or antibody-drug conjugate.5. The method of claim 1 , wherein the deglycosylation reagent is a deglycosylation enzyme.6. The method of claim 1 , further comprising analyzing the eluted glycans by one of CE claim 1 , LC claim 1 , MS claim 1 , and NMR claim 1 , and the combinations thereof.7. The method of claim 1 ...

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Номер записи: 71

03-03-2022 дата публикации

Methods and Compositions for Maintaining the Conformation and Structural Integrity of Biomolecules

Номер: US20220062370A1

Автор: Patel Alpeshkumar P., Seifert Jennifer, Sood Paul R., Zarzycki Ryszard

Принадлежит:

A liquid ink composition includes a liquid phase and particles suspended in the liquid phase, the particles containing a target pharmaceutical or biological agent. The biological activity of the target pharmaceutical or biological agent is preserved upon suspension of the particles in the liquid phase. The liquid phase is capable of solidifying via a solidification process. 1. A liquid ink composition comprising:a liquid phase andparticles suspended in the liquid phase, the particles containing a pharmaceutical or biological agent,wherein the biological activity of the pharmaceutical or biological agent is preserved upon suspension of the particles in the liquid phase,wherein the liquid phase is capable of solidifying to form three dimensional structures.2. The liquid ink composition of claim 1 , the particles being formed by a process comprising: a pharmaceutical or a biological agent; and', 'a substrate that is soluble in the solution, comprising one or more chemical species;, 'preparing a solution, formed from water as a solvent, comprisingcombining the solution with an oil phase to form a water-in-oil emulsion in which the solution is dispersed in the oil phase;lyophilizing the emulsion,wherein the particles are formed prior to or simultaneously with the lyophilizing,wherein the pharmaceutical or biological agent is entrapped by the formed particles,wherein the substrate composition and the oil phase are selected so that the particles formed are suspendable in the liquid ink composition and the biological activity of the pharmaceutical or biological agent is preserved upon suspension of the particles in the liquid ink composition, andwherein one or more substances selected from the group consisting of a surfactant, a stabilizer, an emulsifier, and combinations thereof are incorporated as part of the substrate.3. The liquid ink composition of claim 1 , wherein the biological activity of the pharmaceutical or biological agent is preserved upon solidification of ...

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Номер записи: 72

22-02-2018 дата публикации

Enzymatic synthesis of soluble glucan fiber

Номер: US20180049457A1

Автор: Arthur Ouwehand, Jahnavi Chandra Prasad, Kevin D. Nagy, Mark S. Payne, Qiong CHENG, Robert Dicosimo, Supaporn Suwannakham, ZHENG You

Принадлежит: EI Du Pont de Nemours and Co

An enzymatically produced soluble α-glucan fiber composition is provided suitable for use as a digestion resistant fiber in food and feed applications. The soluble α-glucan fiber composition can be blended with one or more additional food ingredients to produce fiber-containing compositions. Methods for the production and use of compositions comprising the soluble α-glucan fiber are also provided.

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Номер записи: 73

25-02-2021 дата публикации

AQUEOUS IRON CARBOHYDRATE COMPLEXES, THEIR PRODUCTION AND MEDICAMENTS CONTAINING THEM

Номер: US20210054105A1

Автор: Geisser Peter, Philipp Erik, Richle Walter

Принадлежит:

A water soluble iron carbohydrate complex obtainable from an aqueous solution of iron(III) salt and an aqueous solution of the oxidation product of one or more maltrodextrins using an aqueous hypochlorite solution at a pH-value within the alkaline range, where, when one maltodextrin is applied, its dextrose equivalent lies between 5 and 20, and when a mixture of several maltodextrins is applied, the dextrose equivalent of the mixture lies between 5 and 20 and the dextrose equivalent of each individual maltodextrin contained in the mixture lies between 2 and 40, a process for its production and a medicament for the treatment and prophylaxis of iron deficiency conditions. 111-. (canceled)12. An iron (III) carboxymaltodextrin complex wherein said iron (III) carboxymaltodextrin complex comprises polynuclear iron (III)-hydroxide 4(R)-(poly-(1→4)-O-α-D-glucopyranosyl)-oxy-2(R) ,3(R) ,5(R) ,6-tetrahydroxy-hexanoate and has a weight average molecular weight in the range of from 118 kDa to 271 kDa as measured by gel permeation chromatography , and wherein said 4(R)-(poly-(1→4)-O-α-D-glucopyranosyl)-oxy-2(R) ,3(R) ,5(R) ,6-tetrahydroxy-hexanoate is derived from the oxidation of maltodextrin , wherein when one maltodextrin is applied its dextrose equivalent lies between 5 and 20 , and when a mixture of several maltodextrins is applied , the dextrose equivalent of the mixture lies between 5 and 20 and the dextrose equivalent of each individual maltodextrins contained in the mixture lies between 2 and 40.13. The iron (III) carboxymaltodextrin complex of claim 12 , wherein the oxidized maltodextrin is obtained by oxidation of maltodextrin in an aqueous hypochlorite solution.14. A medicament comprising the iron (III) carboxymaltodextrin complex of and at least one pharmaceutically acceptable carrier claim 12 , excipient claim 12 , or additive claim 12 , wherein said medicament is an aqueous solution of said iron (III) carboxymaltodextrin complex.15. The medicament of claim 14 , ...

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Номер записи: 74

22-02-2018 дата публикации

PHARMACEUTICAL COMPOSITIONS COMPRISING MODIFIED FUCANS AND METHODS RELATING THERETO

Номер: US20180051097A1

Автор: Springate Christopher Michael Kevin

Принадлежит: Arc Medical Devices, Inc.

Compositions and methods relating to fucan agents useful for the treatment, prevention, inhibition, etc., of fibrous adhesions or other diseases. 1. A medical composition comprising a therapeutically effective amount of a modified fucan in combination with at least one pharmaceutically acceptable excipient , filler , carrier or diluent , wherein the modified fucan has a sulphate content between about 14 to 40% w/w; a total carbohydrate content between about 37 to 75% w/w; a fucose content as a percentage of a total carbohydrate content of between about 31 to 71%; an acetyl content as a ratio of acetyl: fucos of between about 0 to 20% w/w: a protein content of about 0 to 12% w/w: an appearance of white , off-white , light yellow , light orange , light green or light brown; and when made up to a 0.1% w/v solutions results in a solution with a pH of about 4 to 8.227- (canceled)28. The medical composition of wherein the modified fucan has a molecular weight distribution such that the portion from about 0 to 5 claim 1 ,000 g/mol comprises between about 0 to 25% w/w claim 1 , the portion from about 5 claim 1 ,000 to 60 claim 1 ,000 g/mol comprises between about 0 to 55% w/w claim 1 , the portion from about 60 claim 1 ,000 to 200 claim 1 ,000 g/mol comprises between about 5 to 35% w/w claim 1 , the portion from about 200 claim 1 ,000 to 1 claim 1 ,600 claim 1 ,000 g/mol comprises between about 0 to 50% w/w claim 1 , and the portion from more than about 1 claim 1 ,600 claim 1 ,000 g/mol comprises about 0 to 50% w/w %.29. The medical composition of wherein the modified fucan has a molecular weight distribution such that the portion from about 0 to 5 claim 28 ,000 g/mol comprises between about 0 to 25% w/w claim 28 , the portion from about 5 claim 28 ,000 to 60 claim 28 ,000 g/mol comprises between about 5 to 38% w/w claim 28 , the portion from about 60 claim 28 ,000 to 200 claim 28 ,000 g/mol comprises between about 10 to 30% w/w claim 28 , the portion from about 200 claim ...

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Номер записи: 75

23-02-2017 дата публикации

A NOVEL DOWNSTREAM PROCESS FOR PURIFYING POLYSACCHARIDES

Номер: US20170051080A1

Автор: CHHIKARA Manoj Kumar, GILL Davinder, HANIF Sarmad, JOSHI Neeraj, Sharma Sandeep

Принадлежит:

The present invention relates to a novel process for purifying bacterial polysaccharide. It is an efficient and scalable process for removing impurities from serogroup C (Men-C) polysaccharide which is capable of being used as such in a derivatized form or linked to other molecules, for the preparation of vaccines, more particularly conjugate vaccines for infection. 1. A process for purifying Men C-Polysaccharide , wherein said process comprising the steps of:(a) centrifugation of the fermented harvest to clarify the fermented broth;(b) concentrating the fermented supernatant by ultrafiltration;(c) deacetylation and incubating said concentrated supernatant of step (b) at high temperature;(d) collecting the supernatant of step (c) and subjecting to diafiltration and concentration;(e) purification of diafiltered supernatant of step (d) by chromatography;(f) diafiltration and concentration to obtain purified polysaccharides; and(g) sterile filtration of said purified polysaccharides, wherein the said purification process is completed within 6±1 hours.2. The process as claimed in claim 1 , wherein said step of centrifugation of the fermented harvest is carried at 4500×g to 5000×g.3. The process as claimed in claim 1 , wherein said step of concentration by ultrafiltration is carried out using a by 100 KDa molecular cutoff membrane.4. The process as claimed in claim 1 , wherein said step of deacetylation is carried with NaOH solution at a concentration which ranges from 1 to 1.5 M.5. The process as claimed in claim 1 , wherein said incubation in said deacetylation step is performed at a temperature ranging from 70° C. to 80° C.6. The process as claimed in claim 1 , wherein the incubation in the deacetylation step is performed with a total incubation time ranging from 1 hour 30 mins to 2 hours 30 min.7. The process as claimed in claim 1 , wherein the diafiltration in the collecting step after the deacetylation step is carried out 20 to 25 times with Milli-Q water followed ...

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Номер записи: 76

10-03-2022 дата публикации

Immunogenic compositions

Номер: US20220072118A1

Автор: Alan Cross, Donna Ambrosino, Francis Michon, George Rainer Siber, Raphael SIMON, Richard Malley, Sharon TENNANT, Teresa J. Broering

Принадлежит: Affinivax Inc, University of Maryland at Baltimore

Technologies for the prevention and/or treatment of nosocomial infections.

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Номер записи: 77

15-05-2014 дата публикации

Pectic polysaccharide and method for producing same

Номер: US20140134310A1

Автор: Akihiro Nakamura, Junko Tobe, Norifumi Adachi

Принадлежит: Fuji Oil Co Ltd

The present invention provides a pectic polysaccharide, wherein a degree of methyl esterification of constituent galacturonic acid is 45% or less, a structure of a single molecule observed with an atomic force microscope comprises a star structure, and a diameter of the molecule is more than 100 nm and equal to or less than 200 nm.

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Номер записи: 78

04-03-2021 дата публикации

Method and an apparatus for treating plant based raw material with an enzymatic hydrolysis

Номер: US20210062231A1

Автор: Juha Tamper, Sami Turunen

Принадлежит: UPM Kymmene Oy

A method for treating plant based raw material with an enzymatic hydrolysis. The plant based raw material is treated to form lignocellulosic material. The lignocellulosic material or a solid fraction thereof is subjected to the enzymatic hydrolysis. The method includes treating the plant based raw material in at least one treatment stage for forming the lignocellulosic material including over 80% fine solid particles that are fiber-like or indefinable particles smaller than 0.2 mm and the viscosity of the lignocellulosic material is below 18000 mPas at 15% dry matter content. The method further includes subjecting the lignocellulosic material or at least one solid fraction thereof into the enzymatic hydrolysis for forming a lignin based material. The method further includes subjecting the lignin based material into at least one solid-liquid separation stage after the enzymatic hydrolysis and separating a lignin fraction and a soluble carbohydrate containing fraction.

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Номер записи: 79

04-03-2021 дата публикации

USE OF POLYAMINES AS RESOLVING AGENTS FOR CAPILLARY ELECTROPHORESIS OF LABELED GLYCANS USING GELS

Номер: US20210063347A1

Автор: HAXO Francis T., KIMZEY Michael J., LIMJAP Fielito U.

Принадлежит: AGILENT TECHNOLOGIES, INC.

The present disclosure provides methods of improving the resolution of labeled glycans in capillary electrophoresis techniques using a gel as a sieving matrix, by incorporating polyamines in the gel. 21. The method of method , wherein said polyamine is of Structure 4 and is 1 ,4 ,7-Trimethyl-1 ,4 ,7-triazacyclononane.3. The method of claim 1 , wherein said polyamine is of Structure 1 and is 1 claim 1 ,2-Ethanediamine claim 1 , N1-(2-aminoethyl) or 1 claim 1 ,2-Ethanediamine claim 1 , N1-[2-(dimethylamino) ethyl]-N1 claim 1 , N2 claim 1 , N2-trimethyl-.4. The method of claim 3 , wherein said polyamine is 1 claim 3 ,2-Ethanediamine claim 3 , N1-[2-(dimethylamino) ethyl]-N1 claim 3 , N2 claim 3 , N2-trimethyl-.5. The method of claim 1 , wherein said polyamine is of Structure 2 claim 1 , and is N claim 1 ,N claim 1 ,N′ claim 1 ,N′-Tetramethyl-1 claim 1 ,3-propanediamine6. The method of claim 1 , wherein said glycans have been labeled by being reacted with 8-aminopyrene-1 claim 1 ,3 claim 1 ,6-trisulfonic acid (“APTS”) claim 1 , InstantQ™ claim 1 , or 8-Aminonaphthalene-1 claim 1 ,3 claim 1 ,6-trisulfonic acid disodium salt (“ANTS”).7. The method of claim 6 , wherein the glycans have been labeled with APTS and are MAN-5 glycan and A1F.8. (canceled)9. The method of claim 6 , wherein the glycans have been labeled with InstantQ™ and are MAN-5 glycan and A1F.10. (canceled)12. The composition of claim 11 , wherein said polyamine is of Structure 4 and is 1 claim 11 ,4 claim 11 ,7-Trimethyl-1 claim 11 ,4 claim 11 ,7-triazacyclononane.13. The composition of claim 11 , wherein said polyamine is of Structure 1 and is 1 claim 11 ,2-Ethanediamine claim 11 , N1-(2-aminoethyl) or 1 claim 11 ,2-Ethanediamine claim 11 , N1-[2-(dimethylamino) ethyl]-N1 claim 11 , N2 claim 11 , N2-trimethyl-.14. The composition of claim 11 , wherein said polyamine is N claim 11 ,N claim 11 ,N′ claim 11 ,N″ claim 11 ,N″-Pentamethyldiethylenetriamine or N claim 11 ,N claim 11 ,N′ claim 11 ,N′-Tetramethyl-1 ...

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Номер записи: 80

28-02-2019 дата публикации

Method for preparation of sugammadex sodium

Номер: US20190062459A1

Автор: Ching-Peng Wei, Yu-Liang Liu

Принадлежит: Formosa Laboratories Inc

The present invention provides a method for improved preparation of Sugammadex sodium.

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Номер записи: 81

10-03-2016 дата публикации

GLYCOGEN-BASED WATER SOLUBILITY ENHANCERS

Номер: US20160068615A1

Автор: LIBERATI Elisa, Russo Vincenzo, TONGIANI Serena

Принадлежит: AZIENDE CHIMICHE RIUNITE ANGELINI FRANCESCO A.C.R.A. F. S.p.A.

The present invention relates to glycogen-based polymers and the use thereof for enhancing the solubility in water of lipophilic compounds, to complexes of the said glycogen-based polymers with lipophilic compounds and the use thereof for administering lipophilic compounds, and to pharmaceutical, nutraceutical, and cosmetic compositions comprising the said complexes. 2. The glycogen-based polymer according to claim 1 , wherein said alkyl group has from 2 to 10 carbon atoms.3. The glycogen-based polymer according to claim 1 , wherein said alkyl group has from 2 to 9 carbon atoms.4. The glycogen-based polymer according to claim 1 , wherein said alkyl group has from 2 to 8 carbon atoms.5. The glycogen-based polymer according to claim 1 , wherein said alkyl group has from 4 to 8 carbon atoms.6. The glycogen-based polymer according to claim 1 , wherein said alkenyl group has from 2 to 10 carbon atoms.7. The glycogen-based polymer according to claim 1 , wherein said alkenyl group has from 2 to 8 carbon atoms.8. The glycogen-based polymer according to claim 1 , wherein said alkenyl group has from 4 to 8 carbon atoms.9. The glycogen-based polymer according to claim 1 , wherein said arylalkyl group has from 8 to 16 carbon atoms.10. The glycogen-based polymer according to claim 1 , wherein said arylalkyl group has from 8 to 14 carbon atoms.11. The glycogen-based polymer according to claim 1 , wherein said arylalkyl group has from 10 to 14 carbon atoms.12. The glycogen-based polymer according to claim 1 , wherein said arylalkenyl group has from 8 to 16 carbon atoms.13. The glycogen-based polymer according to claim 1 , wherein said arylalkenyl group has from 8 to 14 carbon atoms.14. The glycogen-based polymer according to claim 1 , wherein said arylalkenyl group has from 10 to 14 carbon atoms.15. The glycogen-based polymer according to claim 1 , wherein each of said groups R claim 1 , which may be identical or different claim 1 , is a hydrogen atom claim 1 , an alkyl group ...

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Номер записи: 82

11-03-2021 дата публикации

CHITIN AND PROCESS FOR PRODUCING CHITIN AND/OR CHITOSAN BY THE ENZYMATIC AND CHEMICAL PATHWAY

Номер: US20210070889A1

Автор: Alezra Valérie, Berezina Nathalie, Laurent Sophie, LE BERRE Corentin, Lorette Bénédicte, Sanchez Lorena, Socolsky Cecilia

Принадлежит: YNSECT

The present invention relates to chitin with a differential purity of more than 97.75% and to a process for producing chitin and/or chitosan by the enzymatic and chemical pathway. 1. Chitin , the purity by difference of which is greater than 97.75% , wherein the purity by difference is obtained by subtraction of the amino acid , lipid and ash impurity contents from the absolute purity value , said absolute purity value being equal to 100%.2. Chitin according to claim 1 , containing less than 1.2% by weight amino acids relative to the total dry weight of chitin.3. Chitin according to claim 1 , containing less than 2% by weight ash relative to the total dry weight of chitin.4. Chitin according to claim 1 , the purity by difference of which is greater than or equal to 98.0% and the molar mass of which is greater than or equal to 800 kg·mol claim 1 , wherein the molar mass is determined using the falling ball viscosity measurement.5. Chitosan claim 1 , the purity by difference of which is greater than 97.75% claim 1 , wherein the purity by difference is obtained by subtraction of the amino acid claim 1 , lipid and ash impurity contents from the absolute purity value claim 1 , said absolute purity value being equal to 100%.6. Chitosan according to claim 5 , the purity by difference of which is greater than or equal to 97.9% and the molar mass of which is greater than or equal to 480 kg·mol claim 5 , wherein the molar mass is determined using the falling ball viscosity measurement.7. Method for obtaining chitin and/or chitosan claim 5 , from insects claim 5 , comprising the following steps:separation of the cuticles from the soft part of the insects,enzymatic hydrolysis of the cuticles by a protease, in order to obtain a solid residue, andbasic treatment of the solid residue.8. Method according to claim 7 , in which the separation of the cuticles from the soft part of the insects is performed using a belt separator.9. Method according to claim 7 , in which the protease is ...

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Номер записи: 83

11-03-2021 дата публикации

METHOD FOR OBTAINING PURIFIED BACTERIAL POLYSACCHARIDES

Номер: US20210070890A1

Автор: DHERE Rajeev Mhalasakant, GAIKWAD Walmik Karbhari, JANA Swapan Kumar

Принадлежит:

The present disclosure relates to a method for obtaining purified bacterial polysaccharides. The method comprises simultaneous removal of impurities as well as sizing of bacterial polysaccharides using an acid instead of conventional mechanical sizing methods. The method is simple, rapid and cost effective. The method results in high polysaccharide recovery and low impurity content. The purified polysaccharide obtained by the method of the present disclosure may be used for large scale production of polysaccharide-protein conjugate vaccines. 1. A method for obtaining purified and sized bacterial polysaccharides , the method comprising the following steps:(a) providing a fermentation harvest comprising bacterial cell, polysaccharide, proteins, nucleic acid and cell debris; and(b) treating the fermentation harvest with an acid to separate the polysaccharide from protein, nucleic acid and cell debris to obtain purified and sized bacterial polysaccharides.2. The method as claimed in claim 1 , wherein the purified and sized bacterial polysaccharides have a protein content less than 3% claim 1 , a nucleic acid content less than 2% claim 1 , and a molecular size in a range of 50 kDa to 600 kDa (SEC-HPLC) claim 1 , wherein the recovery of polysaccharides is at least 60%.3. The method as claimed in claim 1 , wherein the purified and sized bacterial polysaccharides has a polysaccharide polydispersity index/coefficient less than 2 and a CWPs content not more than 2 mol %.4StreptococcusSalmonellaShigellaE. coli, Neisseria meningitidis, Neisseria gonorrhoeae, Haemophilus influenzae, Haemophilus pneumonia, Helicobacter pylori, Chlamydia pneumoniae, Chlamydia trachomatis, Ureaplasma urealyticum, Mycoplasma pneumoniae, StaphylococcusEnterococcus faecalis, Enterococcus faecium, Bacillus anthracis, Vibrio cholerae, Pasteurella pestis, Pseudomonas aeruginosa, CampylobacterClostridiumMycobacteriumMoraxella catarrhalis, Klebsiella pneumoniae, TreponemaBorreliaBorrelia burgdorferi, ...

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Номер записи: 84

07-03-2019 дата публикации

Method for preparing a mixture of monosaccharides and/or of oligosaccharides and/or of polysaccharides via purification of a hydrolysate of lignocellulosic materials

Номер: US20190071463A1

Автор: Christine Chirat, Dominique Lachenal, Jeremy BOUCHER

Принадлежит: Institut Polytechnique de Grenoble

The reuse of the sugars from the by-products of the paper and cellulose industries and lignocellulosic biorefineries and facilitating the extraction and the purification of the sugars contained in the hydrolysates of wood. A method for preparing a mixture of monosaccharides and/or of oligosaccharides and/or of polysaccharides via purification of a hydrolysate of lignocellulosic materials, said hydrolysate comprising hemicelluloses in the form of monomers, of oligomers, and optionally of polymers. The method includes at least one step of oxidation of said hydrolysate with at least one oxidant. This method allows a mixture of monosaccharides and/or of oligosaccharides and/or of polysaccharides to be obtained having a reduced quantity of furfural and/or of hydroxymethylfurfural and comprising polymers having a reduced mass molecular in weight and/or in number.

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Номер записи: 85

05-03-2020 дата публикации

Myrtle Polysaccharide P1, the Separation Method Thereof and the Use in Preparing Hypolipidemic Drugs Therefor

Номер: US20200069724A1

Автор: GAO Linlin, Huang Ruqiang, Wang Jinghui, WANG Qian, Zhang Jingwen

Принадлежит:

The invention discloses a myrtle polysaccharide P1, the separation method thereof and the use in preparing hypolipidemic drugs therefor, wherein the P1 contains 6.74% of ribose, 1.73% of rhamnose, 60.06% of arabinose, 3.54% of xylose, 5.64% of mannose, 13.16% of glucose, and 9.13% of galactose. The experiment result shows that the myrtle polysaccharide P1 has a certain ability to bind cholate in vitro. Taking cholestyramine as a positive control and the binding rate of cholestyramine to each cholate as 100%, the relative binding rate of the myrtle polysaccharide P1 to sodium taurocholate, sodium glycocholate and sodium cholate was 25.28%, 44.56%, and 50.10%, respectively. 1. A myrtle polysaccharide P1 , characterized in comprising the following monosaccharides by molar percentage: 6.74% of ribose , 1.73% of rhamnose , 60.06% of arabinose , 3.54% of xylose , 5.64% of mannose , 13.16% of glucose , and 9.13% of galactose.2. The myrtle polysaccharide P1 according to claim 1 , characterized in having a number average molecular weight of 6.44×10Da and a weight average molecular weight of 2.04×10Da.3. A method for separating and purifying the myrtle polysaccharide P1 according to claim 1 , characterized in comprising the following steps:(1) refluxing the myrtle fruit to obtain a crude myrtle fruit polysaccharide;{'sub': 2', '2, '(2) decolorizing the crude myrtle fruit polysaccharide by adding 30% HOsolution;'}(3) adding the decolorized polysaccharide to a Sevag reagent for deproteinizing to obtain a preliminary purified myrtle fruit polysaccharide;(4) packing a column with pretreated DEAE-Sepharose filler, dissolving the preliminary purified myrtle fruit polysaccharide in step (3) in deionized water, filtering with a 0.45 μm microporous membrane, and then applying to the column; eluting by distilled water, collecting the eluate, detecting the absorbance of the eluate at a wavelength of 490 nm by phenol-sulfuric acid method, stopping the collection when the absorbance is ...

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Номер записи: 86

19-03-2015 дата публикации

HIGHLY BRANCHED ALPHA-D-GLUCANS

Номер: US20150080220A1

Автор: Yao Yuan, Zhang Jingmin

Принадлежит:

Compositions including a highly branched alpha-D-glucan or modified forms thereof and a solute compound are described herein. The compositions can provide increased water solubility and/or increased rate of dissolution for the solute compound. The compositions can also provide increased stability for the solute compound. Methods for preparing and using compositions including a solute compound and a highly branched alpha-D-glucan are also described. 1. A composition for increasing solubility of a solute compound , comprising:a highly branched alpha-D-glucan or a modified form thereof; anda solute compound having an aqueous solubility that is greater than the aqueous solubility of the solute compound in the absence of the highly branched alpha-D-glucan.2. The composition of claim 1 , wherein the highly branched alpha-D-glucan has a percentage branch density greater than about 7%.3. The composition of claim 1 , wherein the highly branched alpha-D-glucan has a dendritic structure.4. The composition of claim 1 , wherein the highly branched alpha-D-glucan is glycogen claim 1 , phytoglycogen claim 1 , or a modified form thereof.5. The composition of claim 1 , wherein the rate of dissolution of the solute compound in aqueous solvent is greater than the rate of dissolution of the solute compound in the absence of the highly branched alpha-D-glucan.6. The composition of claim 1 , wherein the solubility is increased by at least about a factor of two relative to the solubility of the solute compound in the absence of the highly branched alpha-D-glucan.7. The composition of claim 1 , wherein the highly branched alpha-D-glucan is a modified highly branched alpha-D-glucan.8. The composition of claim 7 , wherein the modified highly branched alpha-D-glucan contains at least a chemical group selected from acetate claim 7 , phosphate claim 7 , octenyl succinate claim 7 , succinate claim 7 , hydroxypropyl claim 7 , hydroxyethyl claim 7 , cationic groups claim 7 , carboxymethyl claim 7 ...

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Номер записи: 87

18-03-2021 дата публикации

CHITIN AND METHOD FOR CHEMICALLY OBTAINING CHITIN AND/OR CHITOSAN

Номер: US20210079122A1

Автор: Alezra Valérie, Berezina Nathalie, Laurent Sophie, LE BERRE Corentin, Lorette Bénédicte, Socolsky Cecilia

Принадлежит: YNSECT

The present invention relates to a chitin having a molecular mass of more than 855 kg·mol-1 and to a process for obtaining chitin and/or chitosan by separating cuticles from the soft part of the insect and by a basic treatment of the cuticles. 1. Chitin having a molecular mass greater than or equal to 855 kg·moland containing less than 1.5% by weight amino acids relative to the total dry weight of chitin , wherein the molecular mass is determined using the falling ball viscosity measurement.2. Chitin according to claim 1 , containing less than 3% by weight ash relative to the total dry weight of chitin.3. Chitin according to claim 1 , the purity by difference of which is greater than or equal to 95% claim 1 , wherein the purity by difference is obtained by subtracting the amino acid claim 1 , lipid and ash impurity contents from the absolute purity value claim 1 , said absolute purity value being equal to 100%.4. Chitosan having a molecular mass greater than or equal to 250 kg·mol claim 1 , wherein the molecular mass is determined using the falling ball viscosity measurement.5. Chitosan according to claim 4 , the purity by difference of which is greater than or equal to 95% claim 4 , wherein the purity by difference is obtained by subtraction of the amino acid claim 4 , lipid and ash impurity contents from the absolute purity value claim 4 , said absolute purity value being equal to 100%.6. Method for obtaining chitin and/or chitosan claim 4 , from insects claim 4 , comprising the following steps:separation of the cuticles from the soft part of the insects, thenbasic treatment of the cuticles.7. Method according to claim 6 , in which the separation of the cuticles from the soft part of the insects is performed using a belt separator.8. Method according to claim 6 , in which the separation of the cuticles from the soft part of the insects is performed using a filter press.9. Method according to claim 6 , in which the basic treatment is carried out with a strong base. ...

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Номер записи: 88

14-03-2019 дата публикации

HOMOGENEOUS POLYSACCHARIDE WITH IMMUNOREGULATION ACTIVITY AND PREPARATION METHOD THEREOF

Номер: US20190077886A1

Автор: ZENG Xing

Принадлежит:

The present invention relates to a homogeneous polysaccharide with an immunoregulation activity and a preparation method thereof. The homogeneous polysaccharide with the immunoregulation activity is a single chromatographic peak and has a molecular weight of 6880±50 Da and an optical rotation value of 158.4±0.5°. An infrared spectrum has an α-configuration sugar characteristic absorption peak at 847.6 cm. Hydrogen-proton characteristic chemical shifts in hydrogen spectra of the polysaccharide are δ 5.40, 3.95, 3.84 and 3.61. Carbon signal characteristic chemical shifts in carbon spectra are δ 99.6, 76.6, 73.3, 71.5, 71.1 and 60.4. The homogeneous polysaccharide has an effect of enhancing body immunity, can achieve excellent anti-tumor, antiviral and anti-infection effects and the like in regulation of immunity-related diseases, and has potential development values in drug and health care product industries for treatment and prevention of the diseases. 1. A homogeneous polysaccharide with immunoregulation activity , the homogeneous polysaccharide being a single chromatographic peak , wherein the homogeneous polysaccharide has a molecular weight of 6880±50 Da and an optical rotation value of 158.4±0.5°;{'sup': '−1', 'an infrared spectrum has a characteristic absorption peak at 847.6 cm;'}hydrogen-proton characteristic chemical shifts in hydrogen spectra of the polysaccharide are δ 5.40 (brs), 3.95 (t, J=7.2 Hz), 3.84(m) and 3.61(m); andcarbon signal characteristic chemical shifts in carbon spectra are δ99.6, δ76.6, δ73.3, δ71.5, δ71.1 and δ60.4.2. The homogeneous polysaccharide with immunoregulation activity according to claim 1 , wherein a polysaccharide mass content in the homogeneous polysaccharide is 92-98%;Agilent1200 liquid chromatography is adopted, a refractive index detector RID is used for analyzing, a chromatographic column is TSK-GEL G4000 PWxL, a mobile phase is ultrapure water, a flow velocity is 0.3-1 ml/min, a detector temperature is 30-40° C., a ...

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Номер записи: 89

14-03-2019 дата публикации

Engineered glucosyltransferases

Номер: US20190078062A1

Автор: Ellen D. SEMKE, Jared B. PARKER, Mark S. Payne, Richard Bott, Slavko Kralj, Susan Marie Hennessey, Veli Alkan, Yougen Li

Принадлежит: DANISCO US INC, EI Du Pont de Nemours and Co, Genencor International Inc

Disclosed herein are glucosyltransferases with modified amino acid sequences. Such engineered enzymes synthesize alpha-glucan products having increased molecular weight. Further disclosed are reactions and methods in which engineered glucosyltransferases are used to produce alpha-glucan.

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Номер записи: 90

14-03-2019 дата публикации

Engineered glucosyltransferases

Номер: US20190078063A1

Автор: Ellen D. SEMKE, Jian Ping Lai, Qiong CHENG, Yougen Li

Принадлежит: EI Du Pont de Nemours and Co

Disclosed herein are glucosyltransferases with modified amino acid sequences. Such engineered enzymes exhibit improved alpha-glucan product yields and/or lower leucrose yields, for example. Further disclosed are reactions and methods in which engineered glucosyltransferases are used to produce alpha-glucan.

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Номер записи: 91

24-03-2016 дата публикации

MONODISPERSE GLYCOGEN AND PHYTOGLYCOGEN NANOPARTICLES AND USE THEREOF AS ADDITIVES IN COSMETICS, PHARMACEUTICALS, AND FOOD PRODUCTS

Номер: US20160083484A1

Автор: DUTCHER John Robert, KORENEVSKI Anton, PAPP-SZABO Erzsebet, STUKALOV Oleg

Принадлежит:

Monodisperse glycogen or phytoglycogen nanoparticles are polyfunctional additives suitable for use in aqueous or alcohol-based cosmetic, pharmaceutical, or food formulations. The nanoparticles may be isolated from various sources (such as corn), and are optionally modified with a range of organic moieties (such as octenyl succinic acid). The monodisperse and particulate nature of the glycogen/phytoglycogen is believed to render such materials useful as rheological modifiers (including modulation of thixotropic behaviour), stabilizers of organic and biological materials, and photostabilizers in sunscreens. 1. A method for changing the rheological behavior of a water-based or alcohol-based formulation comprising adding a composition of monodisperse glycogen or phytoglycogen nanoparticles to the formulation.2. The method of claim 1 , wherein the formulation is thixotropic and the change in rheological behavior comprises an increase in rebuilding time.3. The method of claim 1 , wherein the change in rheological behavior comprises imparting thixotropic behavior.4. The method of any one of to claim 1 , wherein the formulation is a dispersion or solution of at least one small molecule claim 1 , polymer claim 1 , biopolymer claim 1 , colloidal particle or an oil.5. The method of claim 4 , wherein the formulation is a water-based formulation.6. The method of claim 4 , wherein the formulation is an alcohol-based formulation.7. The method of claim 6 , wherein the alcohol is ethyl alcohol claim 6 , propyl alcohol claim 6 , isopropyl alcohol claim 6 , ethylene glycol claim 6 , propylene glycol claim 6 , butylene glycol claim 6 , dipropylene glycol claim 6 , ethoxydiglycol claim 6 , glycerol or a combination thereof.8. The method of any one of to claim 6 , wherein the composition has a polydispersity index of less than about 0.3 as measured by dynamic light scattering.9. The method of any one of to claim 6 , wherein at least about 80% by dry weight of the composition is ...

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Номер записи: 92

31-03-2022 дата публикации

Safe bovine heparin, preparation method, and application

Номер: US20220096530A1

Автор: Ana Maria Freire Tovar, Eduardo Prata Vilanova, Luciano Neves De Medeiros, Paulo Antonio de Souza MOURÃO, Rafael Soares De Aquino, Stephan Nicollas Marcin Centena Goulart De Oliveira

Принадлежит: Heptech Pesquisa E Desenvolvimento Ltda

The present invention relates to preparation method for the scale up production of a safe bovine heparin composed by a distinctively selected unfractioned bovine heparin polymers with low 6 -O-desulfated glucosamine content and a porcine-like antico-agulant activity and protamine neutralization, and methods of its production and application. This safe bovine heparin (SB Heparin) has a comparable structure and function to the porcine heparin, the clinical usage reference, preventing clinical usage impairments as a safe pharmaceutical product, allowing its use as interchangeably drugs.

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Номер записи: 93

12-06-2014 дата публикации

VACCINE ADJUVANT, VACCINE COMPOSITION AND METHOD FOR PREPARING A VACCINE ADJUVANT

Номер: US20140161837A1

Автор: Lin Chi-Chien, LU I-Haung, PAN I-Horng, Wu Hsin-Chieh, YAO Hsin-Jan

Принадлежит: INDUSTRIAL TECHNOLOGY RESEARCH INSTITUTE

The disclosure provides a vaccine adjuvant, including a polysaccharide derived from (also named or ) fruiting body, wherein the molecular weight of the polysaccharide is greater than 100 K Da. Furthermore, the polysaccharide is obtained by an extraction process, and the extraction process includes: (a) adding powder of the fruiting body into water to form a mixture; (b) heating the mixture under reflux; (c) after step (b), removing an insoluble matter from the mixture; (d) after step (c), adding ethanol into the mixture to perform a precipitating step and obtain a precipitate; and (e) performing an isolating step to the precipitate to obtain a fraction the molecular weight of which is greater than 100 K Da of the precipitate. 2. The vaccine adjuvant as claimed in claim 1 , wherein the molecular weight of the polysaccharide is between 2.0×10Da and 2.1×10Da.3. The vaccine adjuvant as claimed in claim 1 , wherein the polysaccharide is obtained by an extraction process claim 1 , and the extraction process comprises:{'i': 'Antrodia camphorata', '(a) adding powder of the fruiting body into water to form a mixture;'}(b) heating the mixture under reflux;(c) after step (b), removing an insoluble matter from the mixture;(d) after step (c), adding ethanol to the mixture to perform a precipitating step and obtain a precipitate; and(e) performing an isolating step to the precipitate to obtain a fraction the molecular weight of which is greater than 100 K Da of the precipitate.4. The vaccine adjuvant as claimed in claim 1 , wherein the polysaccharide is capable of activating a dendritic cell.5. The vaccine adjuvant as claimed in claim 1 , wherein the polysaccharide is capable of enhancing a dendritic cell to express a major histocompatibility complex (MHC) class II claim 1 , CD40 and/or CD86.6. The vaccine adjuvant as claimed in claim 1 , wherein the polysaccharide is capable of enhancing a dendritic cell to induce activation of an antigen-specific T cell.7. The vaccine adjuvant ...

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Номер записи: 94

23-03-2017 дата публикации

NEW POLYDEXTROSE MATERIAL

Номер: US20170081427A1

Автор: Stengel Bruno Frederic

Принадлежит:

The present invention discloses a new type of water-soluble polydextrose. This new type of polydextrose contains at least 75% by weight of saccharide molecules having a degree of polymerisation (DP) of 5 or more and characterised in that the non-digestible fiber content is at least 80% by weight. Further, the present invention relates to a process for preparing this new type of polydextrose and to the use of this polydextrose in products such as food products, pharmaceutical products and personal care products. 116-. (canceled)17. A polydextrose fraction comprising saccharide molecules , the polydextrose fraction being a fraction separated from a polydextrose product by chromatographic separation , wherein at least 90% by weight of the saccharide molecules in the polydextrose fraction have a degree of polymerisation of 5 or more , and wherein less than 10% by weight of the saccharide molecules in the polydextrose fraction have a degree of polymerisation of from 1 to 4 , and wherein the non-digestible fiber content of the polydextrose fraction is at least 80% by weight.18. The polydextrose fraction according to claim 17 , wherein at least 70% by weight of saccharide molecules have a degree of polymerisation of 10 or more.19. The polydextrose fraction according to claim 17 , wherein the molecular weight dispersity of the polydextrose fraction is below 2.0.20. The polydextrose fraction according to claim 17 , wherein the molecular weight dispersity of the polydextrose fraction is below 1.8.21. The polydextrose fraction according to claim 17 , wherein the polydextrose has a volume mean diameter that is smaller than 60 μm.22. The polydextrose fraction according to claim 17 , wherein the non-digestible fiber content of the polydextrose fraction is at least 85% by weight.23. The polydextrose fraction according to claim 17 , wherein the non-digestible fiber content of the polydextrose fraction is at least 90% by weight.24. The polydextrose fraction according to claim 17 , ...

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Номер записи: 95

23-03-2017 дата публикации

Optimized method for decontaminating production of glucose polymers and glucose polymer hydrolyzates

Номер: US20170081688A1

Автор: Agnes Denys, Fabrice ALLAIN, Hela Hacine-Gherbi, Mathieu CARPENTIER, Pierre Lanos, Sophie DUVET, Thierry Dupont

Принадлежит: Roquette Freres SA

The present invention relates to a method for decontaminating glucose polymers or the hydrolysates of the pro-inflammatory molecules thereof. Said method includes a) providing glucose polymers or the hydrolysates thereof, b) optionally, detecting or assaying the pro-inflammatory molecules in the glucose polymers or the hydrolysates thereof provided in Step a), and c) carrying out the following purifying steps: i. treatment using an enzymatic preparation having detergent properties and clarification properties; ii. treatment using a pharmaceutical-grade activated carbon with very high adsorption properties and “micropore” porosity; iii. optionally, treatment using a second activated carbon with “mesopore” porosity; iv. passing them over a macroporous adsorbent polymer resin having porosity greater than 100 Angstroms; and v. continuous ultrafiltration at 5 kDa.

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Номер записи: 96

12-03-2020 дата публикации

BOVINE MILK OLIGOSACCHARIDES

Номер: US20200078384A1

Автор: Barile Daniela, German J. Bruce, Lebrilla Carlito B., LoCascio Riccardo, Mills David

Принадлежит:

Oligosaccharides from bovine milk, whey and dairy products, and methods of producing bovine milk oligosaccharides are provided. 1BifidobacteriuminfantisB. breve. A method of treating gastrointestinal tract microbiota imbalances in an infant , wherein the method comprises administering to the infant a sufficient amount of a composition of subsp. or in or in conjunction with an infant formula ,wherein the infant formula comprises one or more of the following fucosylated oligosaccharides as found in bovine milk:an oligosaccharide consisting of 3 Hexose (Hex) moieties, 4 n-acetyl hexosamine (HexNAc) moieties and 1 fucose (Fuc) moiety;an oligosaccharide consisting of 4 Hex moieties, 4 HexNAc moieties, and 1 Fuc moiety;an oligosaccharide consisting of 3 Hex moieties, 5 HexNAc moieties, and 1 Fuc moiety;an oligosaccharide consisting of 5 Hex moieties, 4 HexNAc moieties, and 1 Fuc moiety;an oligosaccharide consisting of 4 Hex moieties, 5 HexNAc moieties, and 1 Fuc moiety; andan oligosaccharide consisting of 3 Hex moieties, 6 HexNAc moieties, and 1 Fuc moiety;and wherein the composition stimulates the production of a bifidobacterial secretion that reduces colonization of enteropathogenic bacteria in the gut of the infant; modulates signals generated by enteroendocrine and gut epithelial cells in the infant; improves at least one biomarker of gut health in the infant; and/or increases gut colonization and persistence of probiotic bacteria in the infant gut.2. The method of wherein the infant formula further comprises one or more oligosaccharides comprising 2 hexose (Hex) moieties and 1 fucose (Fuc) moiety; 4 Hex moieties and 1 Fuc moiety; 2 HEX moieties claim 1 , 1 n-acetyl hexosamine (HexNAc) moiety and 1 Fuc moiety; 3 Hex moieties and 1 HexNAc moiety; 3 Hex moieties and 6 HexNAc moieties; 4 Hex moieties and 3 HexNAc moieties; 3 Hex moieties and 4 HexNAc moieties; 6 Hex moieties and 2 HexNAc moieties; 4 Hex moieties and 4 HexNAc moieties; 3 Hex moieties and 5 HexNAc moieties ...

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Номер записи: 97

12-03-2020 дата публикации

POLYSACCHARIDE SUSPENSION, METHOD FOR ITS PREPARATION, AND USE THEREOF

Номер: US20200079932A1

Автор: HAGER Markus, Kroner Gert, Männer Johann, Opietnik Martina, Redlinger Sigrid

Принадлежит:

The present invention relates to a novel stable colloidal polysaccharide suspension containing α(1→3)-glucan, a cost-effective method for its preparation, and possible uses of these polysaccharide suspensions. 1. A phase-stable , colloidal polysaccharide suspension characterized in that the polysaccharide consists at least partly of (1→3)-glucan , that the α(1→3)-glucan was never dried during its preparation , that the suspension was prepared from a press cake having a polysaccharide content between 4 and 80% by weight , preferably between 15 and 45% by weight , and that the polysaccharide concentration of the suspension is between 0.01 and 50% by weight , preferably between 1.0 and 20% by weight.2. A suspension as claimed in claim 1 , characterized in that the (1→3)-glucan content of the polysaccharide is between 1 and 100% by weight claim 1 , more preferably between 80 and 100% by weight.3. A polysaccharide suspension as claimed in claim 1 , wherein at least 90% of the α(1→3)-glucan consist of hexose units and at least 50% of the hexose units are linked via α(1→3)-glycosidic bonds.4. A polysaccharide suspension as claimed in claim 1 , containing apart from the polysaccharide material 1 to 200% by weight claim 1 , related to the polysaccharide quantity claim 1 , in incorporated additives selected from the group comprising pigments claim 1 , titanium oxides claim 1 , especially substoichiometric titanium dioxide claim 1 , barium sulfate claim 1 , ion exchangers claim 1 , polyethylene claim 1 , polypropylene claim 1 , polyester claim 1 , latex claim 1 , activated carbon claim 1 , polymeric superabsorbents claim 1 , and flame retardants.5. A method for preparing a polysaccharide suspension claim 1 , characterized in thata. the base material used is a press cake of an initially moist polysaccharide material consisting at least partly of α(1→3)-glucan,b. the press cake has a solids content between 4 and 80% by weight (related to the entire press cake), preferably ...

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Номер записи: 98

30-03-2017 дата публикации

BIOPOLYMER EXTRACTION

Номер: US20170088637A1

Автор: Al-Zuhairy Salah, LIN Yuemei, Pronk Mario, van Loosdrecht Marinus

Принадлежит:

In a prior art reactor set up dense aggregates of microorganisms are formed, typically in or embedded in an extracellular matrix. Such may relate to granules, to sphere like entities having a higher viscosity than water, globules, a biofilm, etc. The dense aggregates comprise extracellular polymeric substances, or biopolymers, in particular linear polysaccharides. The present invention is in the field of extraction of a biopolymer from a granular sludge, a biopolymer obtained by such method, and a use of such method. 1. A method for extracting biopolymers from dense aggregates formed by microbial organisms , comprising the steps of(i) providing an anionic biopolymer in sludge,(iic) increasing the pH to 8.0-14.0 under addition of at least 1-20% v/v of at least one of Cl2, OCl— and H2O2, at a suitable temperature and during a suitable time, and(iii) extracting the biopolymer.2. The method according to claim 1 , further comprising the step of (iia) after providing the sludge removing particles with a diameter larger than 500 μm.3. The method according to claim 1 , further comprising the step of (iib) after providing the sludge removing water to a 1-40% w/v content of the wet sludge.4. The method according to claim 1 , further comprising the step of (iiia) reducing the pH by addition of an acid.5. The method according to claim 4 , further comprising the step of after reducing the pH (iiib) removing the sludge by at least one of physical separation claim 4 , settling claim 4 , centrifugation claim 4 , cyclonic separation claim 4 , decantation claim 4 , filtration claim 4 , sieving claim 4 , and flotation claim 4 , under suitable conditions.6. The method according to claim 1 , wherein the biopolymer is bacterial aerobic granular sludge or anammox granular sludge claim 1 , and is selected from exopolysaccharide claim 1 , block-copolymers comprising uronic acid residues claim 1 , alginate claim 1 , lipids claim 1 , and combinations thereof claim 1 , or wherein the ...

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Номер записи: 99

02-04-2015 дата публикации

High purity heparin and production method therefor

Номер: US20150094460A1

Автор: Hiroshi Murata, Michio Muguruma

Принадлежит: Fuso Pharmaceutical Industries Ltd, University of Miyazaki NUC

The present invention provides a high purity heparin useful to be a pharmaceutical product, cosmetics, research reagent, or the like, and a method for producing the same, more specifically, a heparin which does not substantially contain a nitrous acid degradation-resistant impurity and a method for producing a heparin, comprising mixing an aqueous solution of 5 to 30% by weight of the heparin with ethanol having an amount (volume) 0.2 to 1 times the amount (volume) of the aqueous heparin solution to obtain a colloidal precipitate of heparin.

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Номер записи: 100