22-12-2022 дата публикации
Номер: US20220403329A1
Provided are methods of controlling disassociation of cells from a carrier, compositions, and methods of collecting cells. The methods of controlling disassociation of cells from a carrier may include contacting a polymeric carrier with one or more digesting agents to disassociate at least a portion of a plurality of cells from the polymeric carrier. The polymeric carrier may be crosslinked with a crosslinker including at least one of a redox sensitive moiety, a UV light sensitive moiety, a pH sensitive moiety, and a temperature sensitive moiety. 8. The method of claim 1 , wherein the plurality of cells comprises human Mesenchymal stem cells (hMSCs) claim 1 , endothelial cells claim 1 , or a combination thereof.9. The method of claim 1 , wherein the polymeric carrier further comprises dextran claim 1 , hyaluronic acid claim 1 , agarose claim 1 , chitosan claim 1 , alginate claim 1 , polyvinyl alcohol claim 1 , polyamino acid claim 1 , calcium alginate claim 1 , polyacrylate claim 1 , polyethylene glycol claim 1 , polyethylene imine claim 1 , polyanhydride claim 1 , or a combination thereof.10. The method of claim 1 , wherein the polymeric carrier further comprises dextran claim 1 , hyaluronic acid claim 1 , agarose claim 1 , chitosan claim 1 , alginate claim 1 , polyvinyl alcohol claim 1 , polyamino acid claim 1 , or a combination thereof.11. The method of claim 1 , wherein the polymeric carrier is a polymeric carrier bead.12. The method of claim 1 , wherein the one or more digesting agents comprises a reducing agent.13. The method of claim 12 , wherein the reducing agent comprises dithiothreitol (DTT) claim 12 , cysteine claim 12 , glutathione claim 12 , or a combination thereof.14. The method of claim 12 , wherein the reducing agent comprises a solution of dithiothreitol (DTT) having a concentration of about 5 mM to about 25 mM.15. The method of claim 14 , wherein the solution of dithiothreitol further comprises sodium citrate claim 14 , trypsin claim 14 , or a ...
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