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Небесная энциклопедия

Космические корабли и станции, автоматические КА и методы их проектирования, бортовые комплексы управления, системы и средства жизнеобеспечения, особенности технологии производства ракетно-космических систем

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Мониторинг СМИ

Мониторинг СМИ и социальных сетей. Сканирование интернета, новостных сайтов, специализированных контентных площадок на базе мессенджеров. Гибкие настройки фильтров и первоначальных источников.

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Поддерживает ввод нескольких поисковых фраз (по одной на строку). При поиске обеспечивает поддержку морфологии русского и английского языка
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Применить Всего найдено 49737. Отображено 100.
05-01-2012 дата публикации

Sampling devices and methods for concentrating microorganisms

Номер: US20120003626A1
Автор: James E. Aysta, Kurt J. Halverson, Manjiri T. Kshirsagar, Neil Percy
Принадлежит: Individual

The present disclosure describes methods for concentrating microorganisms with concentration agents in a sampling device and the sampling device described herein. More specifically, methods for concentrating microorganisms from large volume samples with concentration agents in a sampling device can provide for rapid, low cost, simple (involving no complex equipment or procedures), and/or effective processes under a variety of conditions.

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Номер записи: 1
05-01-2012 дата публикации

Apparatus and methods for enzymatic debridement of skin lesions

Номер: US20120004627A1
Автор: Amihay Freeman, Eran Hirszowicz, Michal Be'eri-Lipperman
Принадлежит: EnzySurge Ltd, Ramot at Tel Aviv University Ltd

An apparatus for debridement of devitalized tissue in skin lesions, that includes a plurality of height- and angle-adjustable inlet tubes and at least one outlet tube and a member that forms an occlusive seal around a skin lesion. The plurality of inlet tubes is adapted for directing a continuous stream of enzymatic solution to the surface and into the entire volume of the wound bed of the lesion and the at least one outlet is adapted for removing the enzymatic solution, fluids draining from the lesion and tissue debris from the occluded skin lesion.

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Номер записи: 2
27-04-2016 дата публикации

Составной биологический реактор установки для производства биомассы аэробных микроорганизмов

Номер: RU0000161691U1
Автор: Александр Иванович Сафонов
Принадлежит: Александр Иванович Сафонов

1. Составной биологический реактор установки для производства биомассы аэробных микроорганизмов, характеризующийся наличием ориентированной в вертикальной плоскости осесимметричной ферментационной емкости, оснащенной первой обечайкой, которая делит объем осесимметричной ферментационной емкости на первый подъемный и первый опускной каналы, наличием патрубка подвода в осесимметричную ферментационную емкость жидкой минеральной питательной среды, наличием патрубка подвода в нее же воздуха или кислорода, сопряженного с первым барботером, позиционированным в первом подъемном канале, наличием патрубка отвода наружу из осесимметричной ферментационной емкости накопленной биомассы, наличием патрубка первого подъемного и патрубка первого опускного каналов, наличием патрубка отвода из наджидкостного пространства осесимметричной ферментационной емкости паро-газовых продуктов метаболизма, наличием в первом опускном канале теплообменника, прикрепленного к внутренней стенке первой обечайки, наличием ориентированной в вертикальной плоскости, по крайней мере, одной осесимметричной ферментационной емкости-саттелита, оснащенной второй обечайкой, которая делит объем осесимметричной ферментационной емкости-саттелита на второй подъемный и второй опускной каналы, наличием патрубка подвода в осесимметричную ферментационную емкость-саттелит газообразного субстрата, сопряженного с размещенным во втором подъемном канале второго барботера, наличием продувочного патрубка, позиционированного на верхнем торце осесимметричной ферментационной емкости-саттелита, наличием патрубка второго подьемного и патр РОССИЙСКАЯ ФЕДЕРАЦИЯ (19) RU (11) (13) 161 691 U1 (51) МПК C12M 1/02 (2006.01) C12M 1/36 (2006.01) ФЕДЕРАЛЬНАЯ СЛУЖБА ПО ИНТЕЛЛЕКТУАЛЬНОЙ СОБСТВЕННОСТИ (12) ТИТУЛЬНЫЙ (21)(22) Заявка: ЛИСТ ОПИСАНИЯ ПОЛЕЗНОЙ МОДЕЛИ К ПАТЕНТУ 2014149047/10, 20.01.2016 (24) Дата начала отсчета срока действия патента: 20.01.2016 (72) Автор(ы): Сафонов Александр Иванович (RU) (73) Патентообладатель(и): Сафонов Александр ...

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Номер записи: 3
26-01-2012 дата публикации

Belt Agitation System For Culture Bottles

Номер: US20120021452A1
Автор: James C. Bishop
Принадлежит: Biomerieux Inc

A specimen culture container agitation assembly includes an endless belt having a multitude of container-receiving clips attached thereto, each of which is adapted for retaining a specimen culture container to the belt. The belt is oriented vertically. The assembly further includes upper and lower pulleys over which the belt is driven. A drive motor drives the belt around the pulleys. Agitation of the specimen culture container is achieved by rotation of the belt over the pulleys and the retaining of the specimen culture container by the clip.

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Номер записи: 4
26-01-2012 дата публикации

Hydrodynamic extraction of oils from photosynthetic cultures

Номер: US20120021497A1
Автор: Mario C. Larach
Принадлежит: KAIBIOENERGY

The presently described invention relates to the method of extraction of oils from photosynthetic cultures using hydrodynamic cavitation technology for the production of biofuels or other products. This method is referred to herein as Hydrodynamic extraction.

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Номер записи: 5
16-02-2012 дата публикации

Noninvasive Bioreactor Monitoring

Номер: US20120040331A1
Автор: Sarfaraz K. Niazi
Принадлежит: Therapeutic Proteins Inc

A pair or receptacles capable of housing an emitter probe and a detector probe are installed inside a bioreactor to monitor the properties of the nutrient media without contacting the nutrient media.

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Номер записи: 6
23-02-2012 дата публикации

Portable device based on immobilized cells for the detection of analytes

Номер: US20120045835A1
Автор: Aldo Roda, Elisa Michelini, Laura Mezzanotte, Luca Cevenini, Luisa Stella Dolci
Принадлежит: Universita di Bologna

The present invention relates to a portable device for the detection of analytes, in particular toxic substances, comprising at least a whole-cell biosensor with cells immobilized onto a transparent and inert matrix that allows the maintenance of cell vitality. The matrix comprises a mixture of collagen and/or its enzyme degradation derivatives, a proteoglycan and a mixture of vinyl polymer and an optionally modified polysiloxane, and an orthosilicate. The biosensor can be a genetically modified cell that expresses luciferase as reporter gene.

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Номер записи: 7
01-03-2012 дата публикации

Cell evaluation system using cell sheet and method for using the system

Номер: US20120052524A1
Автор: Atsuhiro Saito, Masahiro Kinooka, Masahito Taya, Tatsuya Shimizu, Teruo Okano, Yasunori Takezawa, Yoshiki Sawa
Принадлежит: TOKYO WOMENS MEDICAL UNIVERSITY

Three-dimensional information on a biological parameter relating to at least one selected from viability, proliferating ability, migration, and differentiation of cultured cells can be obtained by a simple two-dimensional analytical technique by constructing a cell evaluation system including a multilayered cell sheet, target cells, and a two-dimensional analyzer.

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Номер записи: 8
01-03-2012 дата публикации

Cell isolation apparatus

Номер: US20120052559A1
Автор: Akane Suzuki, Sunao Takeda, Takahiro SHIOYAMA, Tatsuya Shimizu, Teruo Okano, Yuji Haraguchi
Принадлежит: Nihon Kohden Corp, TOKYO WOMENS MEDICAL UNIVERSITY

A cell isolation apparatus includes: a chamber body which includes a chamber into which tissue is to be introduced; an isolation member which is moved in the chamber and which is to collide with the tissue to isolate a cell; and a controlling portion which controls an operation of the isolation member in the chamber body.

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Номер записи: 9
15-03-2012 дата публикации

Use of gas void fraction measurement is the closed loop control of a fermentation process

Номер: US20120064507A1
Автор: John Biesak
Принадлежит: Cidra Corporated Services LLC

A technique related to a fermentation process; where a signal processor receives a signal containing information about an amount of entrained air in a mixture forming part of a fermentation process in a tank; and determines a level of foam in the tank based at least partly on the amount of entrained air in the mixture. The signal processor may also provide a control signal for controlling an amount of defoamer (or antiforming agent) added to the mixture in the tank so as to control the production of foam within the tank by controlling the amount of defoamer added to the mixture in the tank.

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Номер записи: 10
15-03-2012 дата публикации

Energy photoconverter for obtaining biofuels

Номер: US20120064589A1
Автор: Bernard A. J. Stroiazzo-Mougin, Cristian Gomis Catala
Принадлежит: Individual

The present invention relates to the design of energy photoconverters which act in a continuous and closed manner for producing biofuels and other products of interest by means of the mass culturing of phytoplankton.

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Номер записи: 11
22-03-2012 дата публикации

Electrochemical sensors

Номер: US20120067724A1
Автор: Carolyn R. Kahn, Elicia Wong, Vern Norviel
Принадлежит: Sensor Innovations Inc

Described herein are substrates, sensors and systems related to measuring the concentration of an analyte such as hydrogen ion in a sample. Redox active moieties whose reduction and/or oxidation potentials are sensitive to the presence of an analyte are immobilized onto a surface of an electrode. Immobilized redox active moieties whose reduction and/or oxidation potential are insensitive to the analyte can be used for reference. Voltammetric measurements made using such modified surfaces can accurately determine the presence and/or concentrations of analytes in a sample of interest. The electrochemical sensors of the invention are robust and can be made so as not to require calibration or recalibration.

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Номер записи: 12
29-03-2012 дата публикации

System and method for controlling a fermentation process

Номер: US20120077244A1
Автор: James F. Bartee, Srinivas Budaraju
Принадлежит: Rockwell Automation Technologies Inc

A method for controlling a fermentation process includes injecting a mash into a fermenter and injecting a liquid ammonia additive into the fermenter. The liquid ammonia additive is injected in a closed-loop manner. The method may be used to control the fermentation processes of one or more fermenters operating in parallel.

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Номер записи: 13
12-04-2012 дата публикации

Methods for increasing the production of ethanol from microbial fermentation

Номер: US20120088283A1
Автор: Carl V. Wilkstrom, Ching-Whan Ko, Dinesh K. Arora, Edgar C. Clausen, James L. Gaddy, John Randall Phillips, Rahul Basu
Принадлежит: Ineos Bio Ltd

A stable continuous method for producing ethanol from anaerobic fermentation of a gaseous substrate containing at least one reducing gas in a liquid nutrient medium; supplying the gaseous substrate to the bioreactor. The free acetic acid concentration in the bioreactor is maintained at less than 5 g/L free acid. This method allows ethanol to be produced in the fermentation broth in the bioreactor at a productivity of greater than 10 g/L per day. Both ethanol and acetate are produced in a ratio of ethanol to acetate ranging from 1:1 to 20:1.

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Номер записи: 14
12-04-2012 дата публикации

Photobioreactor system

Номер: US20120088296A1
Автор: Alexandre Stall, Andre Bellin Mariano, Emerson Dilay, Jose Viriato Coelho Vargas, Juan Carlos Ordonez, Wellington Balmont, Zohrob Hovsapian
Принадлежит: Individual

A space efficient photo-bioreactor. The bioreactor grows microalgae in a tall array of transparent flooded tubes. A nutrient media is circulated through the tubes. The array is configured to maximize the amount of sunlight falling upon each tube so that growth of the microalgae is as uniform as possible. Gassing/degassing systems are attached to the array of tubes at appropriate locations. These introduce carbon dioxide and remove oxygen. Cooling systems are preferably also provided so that the circulating media can be maintained at a desired temperature. Microalgae are harvested from the photo-bioreactor. The microalgae is filtered and dried. Lipids are then extracted from the microalgae. These lipids are made into biodiesel through a trans-esterification process. The lipids may be used to make other products as well.

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Номер записи: 15
26-04-2012 дата публикации

Microbially-assisted water electrolysis for improving biomethane production

Номер: US20120100590A1
Автор: Boris Tartakovsky, Serge R. Guiot
Принадлежит: NATIONAL RESEARCH COUNCIL OF CANADA

A method of producing in a bioreactor a biogas rich in methane involves electrolyzing water in an aqueous medium at a voltage in a range of from 1.8 V to 12 V in the presence of electrochemically active anaerobic microorganisms that biocatalyze production of hydrogen gas, and, contacting a species of hydrogenotrophic methanogenic microorganisms with the hydrogen gas and carbon dioxide to produce methane. Volumetric power consumption is in a range of from 0.03 Wh/L R to 0.3 Wh/L R . Current density is 0.01 A/cm E 2 or lower. The voltage is sufficient to electrolyze water without destroying microbial growth. Such a method results in improved electrolysis efficiency while avoiding the use of noble metal catalysts. Further, a combination of water electrolysis with anaerobic degradation of organic matter results in increased biogas quality and in increased biogas quantity and yield. Oxidation of hydrogen sulfide contributes to the increased quality, while an increase in the rate of organic matter hydrolysis and an increase in the production of methane from hydrogen contributes to the increased quantity and yield.

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Номер записи: 16
10-05-2012 дата публикации

Method and device for thermal hydrolysis and steam explosion of biomass

Номер: US20120111515A1
Автор: Odd Egil Solheim, Pål Jahre NILSEN, Paul Walley
Принадлежит: Individual

It is described a process for thermal hydrolysis and team explosion of biomass, said method comprising the steps of: (i) leading the biomass approximately continuous to a first preheating step and preheat the biomass, (ii) leading the preheated biomass into at least two reactors sequentially, (iii) heating and pressurising the reactor by addition of steam, (iv) keeping the re-actor(s) at a certain temperature and pressure for a certain time, (v) leading the heated and pressurised biomass from the reactor(s) to a first pressure relief tank without any substantial pressure reduction and quickly relief the pressure of the biomass by means of a nozzle, in order to disintegrate the biomass, (vi) leading the biomass from the first pressure relief tank to a second pressure relief tank having a lower pressure than the pressure of the first pressure relief tank, (vii) leading the so treated biomass to a downstream facility for subsequent treatment. The invention also comprises a device for thermal treatment of biomass.

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Номер записи: 17
10-05-2012 дата публикации

High fidelity colour imaging of microbial colonies

Номер: US20120114218A1
Автор: Alasdair Graham Hayden-Wright, Philip Atkin
Принадлежит: SYNOPTICS Ltd

A system and an associated method of imaging microbial colonies in high fidelity and in colour. Differently coloured light sources are turned on and off in sequence and for each illumination colour an image is captured by a monochrome camera. The resultant respective monochrome images are combined into a composite colour image of the microbial colonies.

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Номер записи: 18
10-05-2012 дата публикации

Image processing apparatus, incubation observing apparatus, and image processing method

Номер: US20120114219A1
Автор: Hirotada WATANABE, Masato Nakagawa, Yoichi Wada
Принадлежит: KYOTO UNIVERSITY, Nikon Corp

An image processing apparatus includes an outline extracting processing unit inputting a phase contrast image of a cell colony acquired by an observing unit, and extracting an outline of the cell colony, an extracting unit extracting a feature quantity of an outline part of the cell colony based on brightness information at an outside and brightness information at an inside of the outline on the phase contrast image, and an automatic discriminating unit automatically discriminating whether or not the cell colony is an iPS cell colony based on a discriminant criterion determined in advance and the feature quantity extracted by the extracting unit.

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Номер записи: 19
10-05-2012 дата публикации

Container for testing for micro-organisms

Номер: US20120115216A1
Автор: Rosemary Katherine Cameron Sharpin
Принадлежит: ZYZEBA TESTING Ltd

A multi-compartment resealable container for testing for the presence of micro-organisms is provided with or adapted to receive a sample in a first substantially rigid transparent compartment, a growth medium in a second compartment, and a sanitizer in a third compartment, the compartments being separated by foil seals, the second and third compartments have an associated plunger which when depressed causes serrated cutters to puncture the foil seal and allow the contents to be added to the liquid containing or comprising the sample.

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Номер записи: 20
24-05-2012 дата публикации

Acid gas absorbent composition

Номер: US20120128561A1
Автор: Alan M. Blair, Kailas B. Sawant, Keith N. Garside, William J. Andrews
Принадлежит: Individual

This invention provides novel compositions comprising substituted polyamines as acid gas scrubbing solutions and methods of using the compositions in an industrial system. The invention relates to the use of such polyamine compounds in industrial processes to remove acidic contaminants from natural and industrial fluid streams, such as natural gas, combustion gas, natural gas, synthesis gas, biogas, and other industrial fluid streams. The compositions and methods of the invention are useful for removal, absorption, or sequestration of acidic contaminants and sulfide contaminants including CO 2 , H 2 S, RSH, CS 2 , COS, and SO.

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Номер записи: 21
24-05-2012 дата публикации

Device and process for isolating and cultivating live cells on a filter or extracting their genetic material

Номер: US20120129164A1
Автор: Yvon Cayre
Принадлежит: SCREENCELL

The process for isolating live cells on a filter or extracting their genetic material. The process comprises the steps of attaching, at least temporarily, a filter to a lower opening of a compartment having, in addition, an air inlet; inserting into the compartment a liquid carrying the cells; and attaching, in an impermeable manner, a needle, at least temporarily, to the compartment opening, the filter being positioned between the needle and the interior volume of the compartment. The process further comprises the steps of perforation, with the needle, of a plug of a vacuum tube with negative pressure relative to ambient pressure; and aspiration, by means of negative pressure from the vacuum tube, of the liquid through the filter, the filter retaining the cells.

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Номер записи: 22
31-05-2012 дата публикации

Systems and methods for magnetic separation of biological materials

Номер: US20120132593A1
Автор: Aaron Joseph Dulgar-Tulloch, Arvind Kumar Tiwari, James William Bray, Munish Vishwas Inamdar, Shankar Chandrasekaran, Sunil Srinivasa Murthy
Принадлежит: General Electric Co

A magnetic separator comprising a separation chamber is provided. The separation chamber comprises a having an inlet and at least one outlet opposite the inlet in a downstream direction, and a magnetic source operatively coupled to the separation chamber. The magnetic source comprises a plurality of magnets that can be selectively turned off and on to create a dynamic magnetic field in the separation chamber.

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Номер записи: 23
31-05-2012 дата публикации

Apparatus and method of using an agricultural waste digester and biogas generation system

Номер: US20120135492A1
Автор: James E. STRAETER
Принадлежит: Individual

An agricultural waste digester and biogas generation system. The system includes a digester assembly having a cylindrical body, a hollow interior, a center axis and a plurality of wheel segments within the interior of the digester assembly. A gas conduit extends from the interior of the digester assembly to a power generation device. Also included is a water vessel containing water, and each of the plurality of wheel segments have an acruate, contoured surface area which restrict biogas movement within the digester assembly to produce induced agitation of agricultural waste.

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Номер записи: 24
07-06-2012 дата публикации

Real time electronic cell sensing systems and applications for cell-based assays

Номер: US20120142031A1
Автор: Jiangbo Gan, XIAO Xu, Xiaobo Wang, Yama Abassi
Принадлежит: Acea Biosciences Inc

The present invention includes devices, systems, and methods for assaying cells using cell-substrate impedance monitoring. In one aspect, the invention provides cell-substrate impedance monitoring devices that comprise electrode arrays on a nonconducting substrate, in which each of the arrays has an approximately uniform electrode resistance across the entire array. In another aspect, the invention provides cellular assays that use impedance monitoring to detect changes in cell behavior or state. In some preferred aspects, the assays are designed to investigate the affects of compounds on cells, such as cytotoxicity assays.

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Номер записи: 25
07-06-2012 дата публикации

Technique for determining the state of a cell aggregation image processing program and image processing device using the technique, and method for producing a cell aggregation

Номер: US20120142095A1
Автор: Hideki Sasaki, Kazuhiro Yano, Kei Ito, Masafumi Mimura
Принадлежит: Nikon Corp

An image processing program (GP) attains (S 10 ) time lapse images, extracts (S 20 ) a cell aggregation from each of the obtained images, and calculates (S 50 ), for the entire region of the cell aggregation, the spatial distribution in pixel values and temporal change in pixel values of a small region in the extracted cell aggregation. The program determines (S 70 ) the state of change of the cell aggregation toward becoming multi-layered, on the basis of the calculated spatial distribution and time lapse change of pixel values, and outputs (S 80 ) the results of the determination. The image processing program (GP) is configured so as to determine and output, from time lapse images, the state of change of the cell aggregation toward becoming multi-layered.

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Номер записи: 26
14-06-2012 дата публикации

Process For Reducing Contaminants In An Industrial Fluid Stream

Номер: US20120145001A1
Автор: Alan M. Blair, Kailas B. Sawant, Keith N. Garside, William J. Andrews
Принадлежит: Individual

This invention provides novel processes utilizing compositions comprising substituted polyamines as acid gas scrubbing solutions and methods of using the compositions in an industrial system. The invention relates to the use of such polyamine compounds in industrial processes to remove acidic contaminants from natural and industrial fluid streams, such as natural gas, combustion gas, natural gas, synthesis gas, biogas, and other industrial fluid streams. The compositions and methods of the invention are useful for removal, absorption, or sequestration of acidic contaminants and sulfide contaminants including CO 2 , H 2 S, RSH, CS 2 , COS, and SO 2 .

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Номер записи: 27
28-06-2012 дата публикации

Biological microfluidics chip and related methods

Номер: US20120164679A1
Автор: Elwin Xander Vrouwe, Eric Marijn Wielhouwer, Johannes Oonk, Marinus Bernardus Olde Riekerink, Michael Keith Richardson
Принадлежит: UNIVERSITEIT LEIDEN

A biological microfluidics chip ( 100 ) comprising a substrate ( 102 ), a microfluidic inlet port ( 104 ) defining an opening in a surface of the substrate ( 102 ), and a microfluidic outlet port ( 106 ) defining an opening in a surface of the substrate ( 102 ). The biological microfluidics chip ( 100 ) also comprises a plurality of wells ( 108 ) extending from a top surface ( 110 ) of the substrate, wherein each well ( 108 ) is bounded by one or more walls, and an inlet opening ( 112 ) and an outlet opening ( 114 ) are provided in a wall of each of the plurality of wells ( 108 ). An inlet microfluidic channel ( 116 ) is provided in the substrate ( 102 ) to connect the microfluidic inlet port ( 104 ) to each of the inlet openings ( 112 ) in the walls of the wells, and an outlet microfluidic channel ( 118 ) is provided in the substrate to connect each of the outlet openings ( 114 ) in the walls of the wells to the microfluidic outlet port ( 106 ).

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Номер записи: 28
28-06-2012 дата публикации

Asynchronous Magnetic Bead Rotation Sensing Systems and Methods

Номер: US20120164680A1
Автор: Alan Hunt, Brandon H. McNaughton, Irene Sinn, Paivo Kinnunen, Raoul Kopelman, Remy Elbez, Roy Clarke, Theodore Albertson
Принадлежит: University of Michigan

Described herein are various methods, devices and systems for performing asynchronous magnetic bead rotation (AMBR) to detect and monitor cellular growth and/or behavior. Cluster rotation of magnetic particles for AMBR is descried. In particular, described herein are systems for the parallel analysis of multiple wells of a sample plate. Also described herein are methods for controlling the illumination and imaging of rotating magnetic particles.

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Номер записи: 29
12-07-2012 дата публикации

System for producing l-homophenylalanine and a process for producing l-homophenylalanine

Номер: US20120178133A1
Автор: Abdul Latif Ahmad, Pei Ching Oh, Syamsul Rizal Abd. Shukor
Принадлежит: Universiti Sains Malaysia (USM)

The present invention relates to a system ( 10 ) for producing L-homophenylalanine and a process for producing L-homophenylalanine using the system ( 10 ). The system ( 10 ) and the process include monitoring and controlling of the reaction conditions (e.g., temperature and pH) to desired or predetermined values. The monitoring, adjusting and agitating steps provided by the method thereby result in a more complete conversion of the available substrate and produce a sufficient yield of homophenylalanine.

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Номер записи: 30
19-07-2012 дата публикации

Continuous culture apparatus with mobile vessel, allowing selection of fitter cell variants and producing a culture in a continuous manner

Номер: US20120184009A1
Автор: Eudes Francois Marie De Crecy
Принадлежит: Individual

A method and device for growing plant, animal or stem cells in a continuous manner.

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Номер записи: 31
26-07-2012 дата публикации

Cell concentration, capture and lysis devices and methods of use thereof

Номер: US20120190040A1
Автор: Aye Aye Khine, Robert Maaskant, Samad Talebpour, Stephen W. Leonard, Tino Alavie
Принадлежит: QVELLA CORP

The present invention provides a microfluidic devices and methods of use thereof for the concentration and capture of cells. A pulsed non-Faradic electric field is applied relative to a sample under laminar flow, which results to the concentration and capture of charged analyte. Advantageously, pulse timing is selected to avoid problems associated with ionic screening within the channel. At least one of the electrodes within the channel is coated with an insulating layer to prevent a Faradic current from flowing in the channel. Under pulsed application of a unipolar voltage to the electrodes, charged analyte within the sample is moved towards one of the electrodes via a transient electrophoretic force.

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Номер записи: 32
02-08-2012 дата публикации

Method and apparatus for continuous flow membrane-less algae dewatering

Номер: US20120193297A1
Автор: Armin R. Völkel, David K. Fork, Jeonggi Seo, John S. Fitch, Meng H. Lean
Принадлежит: Palo Alto Research Center Inc

In one aspect of the presently described embodiments, the system comprises an inlet to receive at least a portion of the fluid containing algae, a curved channel within which the fluid containing algae flows in a manner such that the neutrally buoyant algae flow in a band offset from a center of the curved channel, a first outlet for the fluid with algae within which the band flows, and, a second outlet for the remaining fluid.

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Номер записи: 33
02-08-2012 дата публикации

Devices and method for enrichment and alteration of cells and other particles

Номер: US20120196273A1
Автор: Bruce L. Carvalho, Lotien Richard Huang, Mehmet Toner, Paul Vernucci, Ravi Kapur, Thomas A. Barber, Zihua Wang
Принадлежит: Individual

The invention features devices and methods for the deterministic separation of particles. Exemplary methods include the enrichment of a sample in a desired particle or the alteration of a desired particle in the device. The devices and methods are advantageously employed to enrich for rare cells, e.g., fetal cells, present in a sample, e.g., maternal blood and rare cell components, e.g., fetal cell nuclei. The invention further provides a method for preferentially lysing cells of interest in a sample, e.g., to extract clinical information from a cellular component, e.g., a nucleus, of the cells of interest. In general, the method employs differential lysis between the cells of interest and other cells (e.g., other nucleated cells) in the sample.

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Номер записи: 34
09-08-2012 дата публикации

Device for monitoring cell culture development

Номер: US20120200305A1
Автор: Matthieu Denoual
Принадлежит: CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS

According to the invention, an electrical heating resistor ( 1 ) is in contact with a receiving surface for heating a cell culture (CC), the resistor being connected to an electric circuit for providing heating power so as to keep the cell culture (CC) at a prescribed temperature setting (Tref). An element ( 61 ) for measuring at least one first parameter (VM), representing the amount of heating power used by the circuit, said measuring element ( 61 ) is also an element ( 61 ) for measuring the development of the cell culture, the device comprising a computer ( 62, 63 ) for calculating a second parameter (PAR), representing the development of the cell culture (CC) over time, on the basis of the first parameter (VM).

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Номер записи: 35
09-08-2012 дата публикации

Treatment system of wet organic waste

Номер: US20120202280A1
Автор: Takaitsu Kobayashi
Принадлежит: URAYASU DENSETSU KK

Provided is a treatment system whereby a wet organic waste can be effectively treated and CO 2 produced in the course of the treatment can be immobilized to thereby contribute to the reduction of CO 2 . The treatment system is characterized by including microbiologically fermenting a wet organic waste in a fermentation device, combusting in a combustion furnace a fermented material obtained from the fermentation device and, at the same time, supplying to the combustion furnace a fermentation gas produced by the microbiological fermentation treatment in the fermentation device, and then recovering and immobilizing CO 2 in a hot exhaust gas produced by the combustion treatment in the combustion furnace.

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Номер записи: 36
16-08-2012 дата публикации

Apparatus and process for production of an encapsulated cell product

Номер: US20120208255A1
Автор: Christine A. Singer, John H. Evans, Iv, Lisa Beckler Andersen
Принадлежит: Geosynfuels LLC

A process for production of an encapsulated cell product, the process comprises the steps of concentrating cells from a propagation medium using a tangential flow filtration system. Mixing the concentrated cells with an encapsulation medium to form a cell encapsulation mixture. Polymerizing, gelling, or cross-linking the cell encapsulation mixture to form an encapsulated cell product.

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Номер записи: 37
20-09-2012 дата публикации

Megakaryocyte and Platelet Production from Stem Cells

Номер: US20120238020A1
Автор: Mauro P. Avanzi, W. Beau Mitchell
Принадлежит: New York Blood Center Inc

Methods for obtaining purified populations of megakaryocytes and platelets by ex vivo culture of stem cells are provided herein.

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Номер записи: 38
04-10-2012 дата публикации

Biomass shock pretreatment

Номер: US20120252070A1
Автор: John J. Dunkleman, Mark A. Deimund, Mark T. Holtzapple, Matthew Falls, Maxine Jones Madison, Rocio Sierra Ramirez
Принадлежит: TEXAS A&M UNIVERSITY SYSTEM

Methods and apparatus for treating biomass that may include introducing a biomass to a chamber; exposing the biomass in the chamber to a shock event to produce a shocked biomass; and transferring the shocked biomass from the chamber. In some aspects, the method may include pretreating the biomass with a chemical before introducing the biomass to the chamber and/or after transferring shocked biomass from the chamber.

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Номер записи: 39
11-10-2012 дата публикации

Apparatus and method for adjusting ph of solution

Номер: US20120255871A1
Автор: Hee-Kyun Lim, Hun-Joo Lee, Jin-Tae Kim, Joon-Ho Kim, Sung-Young Jeong
Принадлежит: SAMSUNG ELECTRONICS CO LTD

A pH adjusting apparatus includes an electrolytic chip receiving a solution, an electrolytic chip loading station receiving the electrolytic chip, an input unit inputting electrolysis conditions, a control unit receiving the electrolysis conditions and controlling electrolysis performed in the electrolytic chip, and a display unit displaying the electrolysis conditions and a progress of the electrolysis. Thus, the pH of a solution can be adjusted easily and accurately, by precisely controlling a constant current, a constant voltage, and current and voltage application times, thereby enabling useful application in various biological assays such as cell lysis. Furthermore, the pH adjusting apparatus has small size and weight and can be operated for a long time after charging once due to low power consumption.

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Номер записи: 40
01-11-2012 дата публикации

Methods for isolating stem cells

Номер: US20120276629A1
Автор: HEE YOUNG LEE
Принадлежит: Individual

Disclosed is a method for isolating stem cells. The method uses a specially designed apparatus including: a container containing an aspirate; a piston having an outer diameter corresponding to the inner diameter of the container and having at least one through-hole; and a connection tube adapted to feed an enzyme or a washing solution into the container through the through-hole, having a tip connected to the through-hole, and connected to an external tube or another container containing the enzyme or washing solution at the other end thereof. The method includes pulling the piston backward to form a negative pressure in the container containing the aspirate and to allow the enzyme or washing solution to enter the container containing the aspirate through the connection tube and the through-hole of the piston.

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Номер записи: 41
01-11-2012 дата публикации

Supplying treated exhaust gases for effecting growth of phototrophic biomass

Номер: US20120276633A1
Автор: Jaime A. Gonzalez, Max Kolesnik, Steven C. Martin
Принадлежит: Pond Biofuels Inc

There is provided a process for growing a phototrophic biomass in a reaction zone. The process includes treating an operative carbon dioxide supply-comprising gaseous material feed so as to effect production of a carbon dioxide-rich product material. The carbon dioxide concentration of the carbon dioxide-rich product material is greater than the carbon dioxide concentration of the operative carbon dioxide supply-comprising gaseous material feed. Production of at least a fraction of the operative carbon dioxide supply-comprising gaseous material feed is effected by a gaseous exhaust material producing process. At least a fraction of the carbon dioxide-rich product material is supplied to the reaction zone so as to effect growth of the phototrophic biomass by photosynthesis in the reaction zone.

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Номер записи: 42
06-12-2012 дата публикации

Simulated Fermentation Process

Номер: US20120310413A1
Автор: David Bluck, Prahsant R. Karbhari, Wen-Jing Lin
Принадлежит: Invensys Systems Inc

A method of modeling a fermentation process comprises providing a first principles model of a fermentation process; determining the concentration of at least one substrate in a fermentation composition at a first time; and predicting the concentration of at least one component of the fermentation composition at a second time using the first principles model, wherein the second time is after the first time.

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Номер записи: 43
13-12-2012 дата публикации

Saccharide-solution producing apparatus, fermentation system, saccharide-solution producing method, and fermentation method

Номер: US20120315677A1
Автор: Gaku Kondo, Hideo Suzuki, Michio Nishiyama, Minoru Genta, Seiichi Terakura
Принадлежит: Mitsubishi Heavy Industries Mechatronics Systems Ltd

A saccharide-solution producing apparatus 11 A according to the present invention is a saccharide-solution producing apparatus for producing a saccharide solution 22 derived from a carbohydrate-based material 21 , and includes a saccharide-solution controlling unit 15 A that controls the saccharide solution derived from the carbohydrate-based material 21 , a cellulosic biomass saccharifying unit 16 that saccharifies hydrothermally treated biomass obtained by hydrothermally decomposing a cellulosic biomass material 35 that contains a lignin component and a hemicellulose component, and produces a diluted saccharide solution 37 , and a diluted-saccharide-solution supply pipe L 11 that mixes the diluted saccharide solution 37 produced by the cellulosic biomass saccharifying unit 16 into the saccharide-solution controlling unit 15 A. With this configuration, it is possible to improve production efficiency of the saccharide solution 22 and to realize cost reduction.

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Номер записи: 44
20-12-2012 дата публикации

Apparatus And Method For Cell Exploitation

Номер: US20120322047A1
Автор: Bassil Akra
Принадлежит: Ludwig Maximilians Universitaet Muenchen LMU

The present invention relates to an apparatus ( 1 ) for automated exploitation of cells from a sample, comprising a plurality of dispensing containers ( 2 ) for the separated storage of fluid media, each dispensing container comprising an outlet ( 21 ) wherein a media delivery arrangement ( 3 ) is associated to each of the dispensing containers ( 2 ) and a first valve ( 22 ) is connected to each of the outlets ( 21 ), a plurality of collecting containers ( 4 ) for the collection of fluid media, each collecting container ( 4 ) comprising an inlet ( 41 ) wherein a second valve ( 42 ) is connected to the inlet ( 41 ) of each collecting container, and a mounting device ( 5 ) for mounting the sample, the mounting device comprising an inlet ( 51 ) being connected to the first valve ( 22 ), and an outlet ( 52 ), wherein the outlet ( 52 ) of the mounting device is connected to the second valve ( 42 ). Further, the present invention enables a method for automated cell exploitation from a sample by the use of the apparatus ( 1 ).

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Номер записи: 45
20-12-2012 дата публикации

Device and method for culturing cells in a biomimetic environment

Номер: US20120322097A1
Автор: Else FROHLICH, Jeffrey T. Borenstein, Joseph L. Charest
Принадлежит: Charles Stark Draper Laboratory Inc

Devices and methods are disclosed herein for a biomimetic flow apparatus. The biomimetic flow apparatus includes a microfluidic flow channel and an inlet in fluid connection with the flow channel for allowing fluid to flow into the flow channel. The flow channel has at least one surface with a topography formed therein. The topography of the at least one surface of the flow channel is selected to cause cells in a cell layer above the surface to achieve an arrangement, behavior, or morphology. The cell arrangement, behavior, or morphology is determined at least in part by the topography of the at least one surface.

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Номер записи: 46
27-12-2012 дата публикации

Combined acoustic micro filtration and phononic crystal membrane particle separation

Номер: US20120325747A1
Автор: Bart Lipkens, Edward A. Rietman, Jason Dionne
Принадлежит: Flodesign Sonics Inc

A system is provided that includes one or more acoustic microfilters through which is flowed a mixture of a fluid and a particulate to selectively filter particles from the fluid. Also included are one or more phononic crystal units coupled to the acoustic microfilter(s) to further selectively filter particles from the fluid. Related apparatus, systems, techniques and articles are also described.

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Номер записи: 47
27-12-2012 дата публикации

Method of culturing eukaryotic cells

Номер: US20120329151A1
Автор: Dinesh Baskar, Inn H. Yuk, Jenny Hsiung, Woon-Lam Susan Laung
Принадлежит: F Hoffmann La Roche AG

An apparatus and method to maintain pH within a range conducive for cell growth in a bicarbonate-containing cell culture system without the addition of base. The method relies on the gas transfer characteristics of the bioreactor system to modulate the CO2 transfer to and from the cell culture such that the pH of the cell culture can be maintained within a desired range.

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Номер записи: 48
03-01-2013 дата публикации

Method for the adsorptive drying of purified biogas and for regenerating laden adsorbents

Номер: US20130000486A1
Автор: Lothar Guenther
Принадлежит: DGE Dr Ing Guenther Engineering GmbH

In a method for adsorptively drying purified biogas and regenerating laden absorbents, foreign matter is not allowed to enter the purified biogas, the content of methane in the gas remains virtually unchanged and the effort involved in regenerating the laden adsorbent is reduced. Drying and regeneration are effected in a closed biogas cycle, wherein separate layers based on silica gel and molecular sieves are used as the adsorbent. The biogas to be dried first flows through the silica gel layer. The adsorbent is regenerated with exclusively heated, dried biomethane having a temperature of up to 150° C. which, after contact with adsorbent, is recirculated to the outflow of purified biogas. After regeneration, the bed is cooled by biomethane, which is subsequently recirculated to the outflow of purified biogas. Methane-containing water accumulating during drying and regeneration is recirculated to the biogas generation and/or purification.

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Номер записи: 49
03-01-2013 дата публикации

Apparatus and method for processing biological material

Номер: US20130005032A1
Автор: James C. Laird, Kyungyoon Min, Thomas E. Dudar
Принадлежит: Baxter International Inc

The application discloses an apparatus and method for processing biological material, including a suspension of cells.

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Номер записи: 50
10-01-2013 дата публикации

Incubator device and method of operating it

Номер: US20130011905A1
Автор: Charli Kruse, Daniel Rapoport, Dennis Wendt
Принадлежит: Fraunhofer Gesellschaft zur Forderung der Angewandten Forschung eV

A movable incubator facility comprises a culture chamber which is arranged for receiving at least one cell culture, a heating arrangement by means of which the temperature of the culture chamber can be adjusted, a gas feed arrangement by means of which at least one gas can be introduced into the culture chamber, and an energy storage arrangement which is disposed for supplying the heating arrangement and/or the gas feed arrangement with electrical energy.

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Номер записи: 51
24-01-2013 дата публикации

Clamping insert for cell culture

Номер: US20130022500A1
Автор: Martha Liley, Silvia ANGELONI SUTER
Принадлежит: Centre Suisse dElectronique et Microtechnique SA CSEM

A clamping insert for cell culture includes: a lower support having: i. a hollow member sized to be engaged in a well of a microplate, the hollow member being open at both its ends and including a support surface at one of its ends, ii. first tightening elements, an inner holder sized to be fitted inside the hollow member, and including second tightening elements arranged to cooperate with the first tightening elements of the hollow member to tighten reversibly between the inner holder and the support surface of the lower support a porous substrate, the inner holder including an opened channel keeping free the porous substrate; retaining elements intended to cooperate with the well of the microplate to allow the clamping insert to be suspended in the well, sealing elements to hermetically seal the contact area between the clamping insert and the porous substrate.

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Номер записи: 52
31-01-2013 дата публикации

Control of Biogas Plants

Номер: US20130029315A1
Автор: Kai Rostalski, Peer Springer
Принадлежит: Ksb Ag

A method and an apparatus for producing biogas from organic matter including a container ( 1 ) which is charged with fermentation substrate by a delivery system ( 13 ). At least one stirring mechanism ( 2 ) is arranged in the container. The feedback value of at least one measurable variable is detected and transmitted to a control unit ( 4 ). A reference variable is also provided to the control unit. The control unit calculates the deviation of the feedback value from the reference value, and actuating variables which modify the power input of the stirring mechanism and/or the composition of the container contents and/or the flow behavior of the container contents are adjusted as a function of the deviation.

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Номер записи: 53
07-03-2013 дата публикации

Method and system for the production of cells and cell products and applications thereof

Номер: US20130058907A1
Автор: Beverly Norris, Darrell P. Page, Grant Adams, Karl Patrick Bongers, Mark Hirschel, Martin Peder Crep, Michael J. Gramer, Robert J. Wojciechowski, Scott Waniger, Thiem Chan Duong Wong
Принадлежит: Biovest International Inc

A cell culture system for the production of cells and cell derived products includes a reusable instrumentation base device incorporating hardware to support cell culture growth. A disposable cultureware module including a cell growth chamber is removably attachable to the instrumentation base device. The base device includes microprocessor control and a pump for circulating cell culture medium through the cell growth chamber. The cultureware module is removably attached to the instrumentation base device. Cells are introduced into the cell growth chamber and a source of medium is fluidly attached to the cultureware module. Operating parameters are programmed into the microprocessor control. The pump is operated to circulate the medium through the cell growth chamber to grow cells or cell products therein. The grown cells or cell products are harvested from the cell growth chamber and the cultureware module is then disposed.

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Номер записи: 54
07-03-2013 дата публикации

Recovery of higher alcohols from dilute aqueous solutions

Номер: US20130059370A1
Автор: Aharon M. Eyal, Andrew C. Hawkins, Aristos A. Aristidou, David A. Glassner, Fudu Miao, Kent Evans, Patrick R. Gruber, William A. Evanko
Принадлежит: Gevo Inc

This invention is directed to methods for recovery of C3-C6 alcohols from dilute aqueous solutions, such as fermentation broths. Such methods provide improved volumetric productivity for the fermentation and allows recovery of the alcohol. Such methods also allow for reduced energy use in the production and drying of spent fermentation broth due to increased effective concentration of the alcohol product by the simultaneous fermentation and recovery process which increases the quantity of alcohol produced and recovered per quantity of fermentation broth dried. Thus, the invention allows for production and recovery of C3-C6 alcohols at low capital and reduced operating costs.

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Номер записи: 55
14-03-2013 дата публикации

System for the treatment of biomass

Номер: US20130065289A1
Автор: David Charles Carlson
Принадлежит: Poet Research Inc

A system for treating biomass for the production of ethanol is disclosed. A biorefinery for producing a fermentation product from biomass is disclosed. The biorefinery comprises a system for preparing the biomass into prepared biomass and a system for pre-treating the biomass into pre-treated biomass. The biorefinery comprises a separator, a first treatment system, a second treatment system, and a fermentation system. A method for producing a fermentation product from biomass is disclosed.

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Номер записи: 56
21-03-2013 дата публикации

Method and device for the absorptive removal of carbon dioxide from biogas

Номер: US20130071307A1
Автор: Stephan Engelke, Uwe Jordan
Принадлежит: MT Biomethan GmbH

The absorptive removal of carbon dioxide from biogas using a scrubbing liquid in which carbon dioxide is chemically bound proceeds by heating the loaded scrubbing liquid, occurring after the absorption, to a temperature at which liberation of CO 2 begins. Immediately thereafter, the loaded scrubbing liquid is fed to at least one centrifugal separator for separating off the gas phase from the liquid phase, wherein methane and dissolved fractions of CO 2 escape via the gas phase. After separation is complete, the gas phase is passed into the absorber unit and the liquid phase is further heated to the temperature required for desorption and fed to the desorption unit for regeneration.

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Номер записи: 57
21-03-2013 дата публикации

Method And Apparatus For Performing Mass Spectrometry

Номер: US20130071867A1
Автор: Fadgen Keith
Принадлежит: WATERS TECHNOLOGIES CORPORATION

The invention feature devices and methods for preserving and processing samples comprising a fluid having deuterated compounds. The device of the present invention comprises a housing defining a first chamber and a second chamber. The first chamber is heated to an elevated temperature and receives the sample and performs a digestion process on the sample at the elevated temperature. The second chamber is cooled to a low temperature and receives the deuterated digested sample from the first chamber and performs one or more separation steps to isolate an analyte. The device of further comprises conduit means for containing and moving the sample into the first chamber to form a digested sample. The conduit means moves the digested sample from the first chamber to the second chamber to separate the sample to form at least one analyte. The analyte is maintained at the low temperature to preserve its deuterated form. 1. A device for processing one or more samples comprising a fluid having one or more deuterated compounds comprising:a. a housing having one or more walls defining at least a first chamber and a second chamber, said first chamber for receiving said one or more samples and performing a digestion process on said sample at an elevated temperature, said second chamber for receiving said sample from said first chamber and performing one or more separation steps to isolate an analyte for further processing at a low temperature;b. conduit means for containing and moving said sample having one or more deuterated compounds into said first chamber to form a digested sample having one or more deuterated digestion products and moving said digested sample from said first chamber to said second chamber to separate said one or more deuterated digestion products to form at least one analyte if present, and said deuterated digestion products and analyte being maintained at said low temperature to preserve said deuterated digestion products and analyte.2. The device of wherein ...

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Номер записи: 58
21-03-2013 дата публикации

Method and Apparatus for Iterative Lysis and Extraction of Algae

Номер: US20130072701A1
Автор: Boggs Tabitha, Chew Geoffrey, Doherty Stephen J., Dykes, JR. H. Waite H.
Принадлежит: Streamline Automation, LLC

A method and system for processing algae involves the use of an ionic liquid-containing clarified cell lysate to lyse algae cells. The resulting crude cell lysate may be clarified and subsequently used to lyse algae cells. The process may be repeated a number of times before a clarified lysate is separated into lipid and aqueous phases for further processing and/or purification of desired products. 1. A method for processing algae cells comprising:a) mixing algae cells with a first clarified algal cell lysate to form a crude algal cell lysate;b) removing solid debris from said crude algae cell lysate to form a second clarified algal cell lysate;c) forming separate lipid-containing hydrophobic and ionic liquid-containing hydrophilic phases in the second clarified algal cell lysate; andd) isolating the lipid-containing hydrophobic phase from the ionic liquid-containing hydrophilic phase;wherein the first and second clarified algae cell lysates each comprise at least 65% hydrophilic ionic liquid by weight andwherein the total amount of water in the crude algal cell lysate formed in step a) is not more than 35% by weight.2. The method of claim 1 , and further comprising removing water from the algae cells before mixing with the clarified algal cell lysate.3. The method of claim 1 , and further comprising mixing a fatty acid with the second clarified algal cell lysate before forming separate lipid-containing hydrophobic and ionic liquid-containing hydrophilic phases.4. The method of claim 3 , wherein said fatty acid is added in an amount of at least 0.7% of the second clarified algal cell lysate by weight.5. The method of claim 1 , wherein algae cells and first clarified algal cell lysate are mixed in a weight ratio of between 1:5 and 1:20.6. The method of claim 1 , wherein said mixing of algae cells and first clarified algal cell lysate is performed at a temperature of between 20° C. and 50° C. for a duration of between 1 and 60 minutes.7. The method of claim 1 , and ...

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Номер записи: 59
28-03-2013 дата публикации

Method and system for the gas-tight process control of percolators in a biogas method having two or more stages

Номер: US20130078700A1
Автор: Grossmann Jochen
Принадлежит: GICON GROSSMANN INGENIEUR CONSULT GMBH

The invention relates to a method and a system for obtaining biogas in two or more stages in a hydrolysis and a methane stage, wherein the hydrolysis of solid biogenic materials is performed in at least two percolators operated at offset times. Liquid hydrolyzate and COrich hydrolysis gas, and then hydrolysis gas comprising methane thereby arises in the percolator. The liquid hydrolyzate is removed from the percolators, wherein part of the hydrolyzate is fed into the methane stage and the other part of the hydrolysis stage. In the methane stage, the hydrolyzate is converted to biogas and fermenting fluid. In the method according to the invention, the percolators are operated in a gas tight manner and hydrolysis gas is drawn off from the percolators, wherein the hydrolysis gas comprising methane is fed to an energy utilization and COrich hydrolysis gas is used for purging a further percolator operated at an offset time. The system according to the invention is suitable for performing said method and comprises at least two gas tight percolators that are interconnected by means of the gas supply lines thereof, and at least one methane reactor. 1. A method for obtaining biogas in two or more stages by hydrolysis of solid biogenic material in at least two percolators operated at offset times , comprising a hydrolysis stage and a methane stage , the method comprising:{'sub': '2', 'hydrolyzing biogenic material in the hydrolysis stage to form in the percolator liquid hydrolysate and hydrolysis gas at first CO-rich hydrolysis gas being formed and afterwards methane-containing hydrolysis gas being formed,'}removing the hydrolysate from the percolators and collecting the hydrolysate,feeding one part of the hydrolysate into the methane stage and feeding the other part of the hydrolysate into the hydrolysis stage,converting the hydrolysate in the methane stage into biogas and fermentation liquid by methane-forming microorganisms,removing the fermentation liquid from the methane ...

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Номер записи: 60
28-03-2013 дата публикации

DEVICE FOR VIRAL INACTIVATION OF LIQUID MEDIA

Номер: US20130078702A1
Автор: Dhanasekharan Muthukumar
Принадлежит: Genzyme Corporation

An apparatus () capable of viral inactivation of liquid media includes at least one coaxial cylinder () constructed of an outer cylinder () and an inner cylinder (), a liquid media inlet (), at least one emitter of type C ultraviolet radiation (), and a liquid media outlet (). The inner cylinder has an outer diameter adapted to form a gap () between the outer diameter of the inner cylinder and the inner diameter of the outer cylinder. The media flows in a substantially cyclonic flow path along the gap. The at least one emitter of type C ultraviolet radiation is placed inside the inner cylinder. The outlet is connected to the outer cylinder at, or proximal to, an end of the outer cylinder opposite the inlet. 1. An apparatus capable of viral inactivation of cell culture media comprising: i) an outer cylinder having a length, an inner diameter, and an outer diameter;', 'ii) an inner cylinder coaxial with the outer cylinder, having a length substantially equal to the length of the outer cylinder and having an outer diameter adapted to form a gap between the outer diameter of the inner cylinder and the inner diameter of the outer cylinder through which the cell culture media flows in a substantially cyclonic flow path along the gap;, 'a) at least one coaxial cylinder comprisingb) a cell culture media inlet connected to the outer cylinder proximal to an end of the outer cylinder and configured to flow the cell culture media along the substantially cyclonic flow path along the gap;c) at least one emitter of type C ultraviolet radiation placed inside the inner cylinder so as to emit the type C ultraviolet radiation towards the cell culture media to be treated with the type C ultraviolet radiation and thereby inactivate viruses in the cell culture media; andd) a cell culture media outlet connected to the outer cylinder proximal to an end of the outer cylinder opposite the inlet.2. The apparatus of claim 1 , wherein the cell culture media flows along the gap at a flow rate in ...

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Номер записи: 61
28-03-2013 дата публикации

CONTINUOUS OR SEMI-CONTINUOUS FLOW PHOTOBIOREACTOR AND METHOD OF USE

Номер: US20130078708A1
Автор: Roux Dit Buisson Jean-Louis
Принадлежит:

The present invention is directed to a photobioreactor for continuous or semi-continuous flow culturing photosynthesizing biomass and related method. In particular, the invention relates to a photobioreactor for continuous or semi-continuous flow culturing photosynthesizing algal biomass and related method. The photobioreactor comprises a gas feeding portion (), a biomass-directing flow propulsion device () on a lighting system (). 112-. (canceled)1325886093234751819. A photobioreactor for continuous or semi-continuous flow culturing photosynthesizing biomass , the photobioreactor including a housing () defining an interior chamber () comprising a culture chamber portion () and a gas feeding chamber portion () , a temperature regulation system () , a flow-regulating system () comprising a culture mixing and concentrating entity () comprising a biomass-directing flow propulsion device () and a lighting system () , the culture chamber portion comprising a culture concentrating zone () and a culture harvesting zone () , the temperature regulation system , the flow-regulating system being within the culture chamber portion , the lighting system being within the culture concentrating zone and the biomass-directing flow propulsion device causing an at least partially vertical flow of culture medium contained in the housing such that the biomass is concentrated in the culture concentrating zone.141247. The photobioreactor according to claim 13 , further comprising a gas feeding system () claim 13 , a feeding device () and a harvesting system ().156. The photobioreactor according to claim 13 , further comprising a feed-back control unit ().16526272857cc. The photobioreactor according to claim 13 , wherein the lighting system () comprises a light source holder device () claim 13 , a light controlling unit () and a rotating support () having a drive ().1726293018. The photobioreactor according to claim 16 , wherein the light source holder device () comprises at least one ...

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Номер записи: 62
28-03-2013 дата публикации

Systems and Methods for Processing Fluids

Номер: US20130079251A1
Автор: Boles T. Christian
Принадлежит: SAGE SCIENCE, INC.

Systems and methods for processing fluid samples are disclosed. Fluid sample processing is accomplished using a series of microfluidic bump arrays include an automated and integrated system for sorting particles from a biological sample, lysing those particles to expose total RNA or DNA, purifying the RNA or DNA, processing the RNA or DNA by chemical or enzymatic modification, to select RNA or DNA molecules by size, or to generate, optionally, a sequencing library. The sequencing library is suitable for use in next generation sequencing (“NGS”). 1. A method for processing of a biological fluid , comprising:separating at least one first cell from the biological fluid;applying at least one first treatment to the at least one separated cell to produce a first treated solution;applying at least one second treatment to the first treated solution to produce a second treated solution; andprocessing at least one of the first treated solution and the second treated solution using a deterministic lateral displacement to generate an output solution.2. The method according to claim 1 , wherein the biological fluid includes at least one of the following: whole blood claim 1 , urine claim 1 , spinal fluid claim 1 , saliva claim 1 , buccal swabs claim 1 , sputum claim 1 , bronchial lavage claim 1 , gastric lavage fluid claim 1 , microbial culture media claim 1 , feces claim 1 , huffy coat claim 1 , serum claim 1 , plasma claim 1 , platelet concentrate claim 1 , water samples claim 1 , and/or any other biological claim 1 , chemical claim 1 , and/or biochemical fluids and/or any combination thereof.3. The method according to claim 1 , wherein the deterministic lateral displacement uses at least one bump array to process at least one of the first treated solution and the second treated solution claim 1 ,4. The method according to claim 1 , wherein the deterministic lateral displacement uses a sequential arrangement of a plurality of bump arrays to process at least one of the first ...

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Номер записи: 63
04-04-2013 дата публикации

MULTI-REACTOR UNIT FOR DYNAMIC CELL CULTURE

Номер: US20130084632A1
Автор: Baudoin Régis, Leclerc Eric, Legallais Cécile, Paullier Patrick, Prot Jean-Matthieu
Принадлежит:

The present invention relates to a unit () comprising a microsystem () and at least one set of interface connections () for the microsystem (); the microsystem () comprising a bottom plate () bearing the impression of at least one microstructured dynamic cell culture chamber (), and a top plate (), characterized in that the at least one microstructured chamber () is fluidically connected at inlet and/or at outlet by a set of connections () inserted removably into a hole in the upper plate (). The present invention also relates to a dynamic cell culture system to this effect. 1115016150150152151511516151. A box () comprising a microsystem () and at least one set of interface connectors () of the microsystem () , the microsystem () comprising a lower plate () bearing the impression of at least one microstructured chamber of dynamic cellular culture () , and an upper plate () , wherein the at least one microstructured chamber of dynamic cellular culture () is connected fluidically at an inlet and/or at an outlet by the at least one set of interface connectors () inserted detachably in a hole of the upper plate ().210151016. The box as claimed in the preceding claim , wherein said box further comprises at least one well () , the microstructured chamber of dynamic cellular culture () being connected to said well () via one set of the at least one set of interface connectors ().31015. The box as claimed in the preceding claim , wherein the bottom of the well () is at an altitude higher than any point of the microstructured chamber of dynamic cellular culture ().4161110. The box as claimed in the preceding claim , wherein the one set of interface connectors () is fixed to a hole () at the bottom of the well ().515101010161016abab. The box as claimed in wherein each of said microstructured chamber of dynamic cellular culture () is connected to an inlet well () and an outlet well () claim 2 , and wherein said inlet well () is perfused by a set of inlet connectors () claim 2 ...

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Номер записи: 64
11-04-2013 дата публикации

Single Use Centrifuge System for Highly Concentrated and/or Turbid Feeds

Номер: US20130089917A1
Автор: Kessler Stephen B., Marro T. David
Принадлежит: PNEUMATIC SCALE CORPORATION

A method and apparatus for cell harvest of production scale quantities of cell cultures using single use components comprising a flexible membrane mounted on a rigid frame and is supported within a multiple use rigid centrifuge bowl, such single use components including a core with an increased diameter and an internal truncated cone shape in order to permit the system to maintain a sufficiently high angular velocity to create a settling velocity suited to efficiently processing highly concentrated cell culture streams. Features which minimize feed turbidity, and others which permit the continuous or semi-continuous discharge of cell concentrate, increase the overall production rate over the rate which can be achieved using current intermittent processing methods for large cell culture volumes. Injection of a diluent during the cell concentrate removal process permits more complete removal of viscous cell concentrates. 1. A centrifuge system for centrifugal separation of a high turbidity feed in a large cell culture batch , having a single use component comprising: a rotatable upper flange, wherein the upper flange includes a central opening,', 'a rotatable lower flange, and', 'a rotatable hollow cylindrical core having an axis, an interior, and an exterior, and which is attached to and extends between the upper flange and the lower flange, wherein the interior of the core is in fluid connection with the exterior of the core,, 'a core structure which is at least semi-rigid and which includes'}a rotatable flexible liner which is sealed, with respect to fluids, to the upper flange and the lower flange,a separation chamber, axially aligned with and surrounding the core and bounded by the flexible liner, having an outer wall which at least partly contains the liner and an inner wall which at least partly contains the exterior of the core, and having an upper end adjacent the upper flange and a lower end adjacent the lower flange, a central feed tube which has an open ...

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Номер записи: 65
11-04-2013 дата публикации

CULTURE APPARATUS HAVING HEATERS

Номер: US20130089924A1
Автор: Busujima Hiroki, KIKUCHI Yasuhiro, YAMASAKI Naoyuki
Принадлежит: PANASONIC HEALTHCARE CO., LTD.

A culture apparatus includes a heat insulating box main body having an inner box and a heat insulating door, a culture vessel, a duct and a circulation blower forcing convection of a gas such as air in the culture vessel, a humidifying pan disposed on a bottom portion of the culture vessel within the duct, and heaters disposed on the outer side of the inner box and on the inner side of the heat insulating door, for heating the inside of the culture vessel. The culture apparatus is configured such that a rapid heater is provided on the downstream side of the circulation blower inside the duct, and the rapid heater is activated if a temperature in the culture vessel is decreased below a prescribed temperature, thereby rapidly regaining the prescribed temperature. 1. A culture apparatus comprising:a heat insulating box main body having an opening on a front side thereof;a heat insulating door attached to the heat insulating box main body in an openable and closable manner;a transparent inner door that seals the opening in an openable and closable manner;a culture vessel for culturing a sample such as cells or microorganisms, the culture vessel being surrounded by the inner door and the heat insulating box main body;a duct and a circulation blower that are used to cause forced convection of a gas such as air in the culture vessel; anda humidifying pan disposed on a bottom portion of the culture vessel within the duct, the humidifying pan being configured to store humidifying water used to control humidity inside the culture vessel, whereinthe heat insulating box main body includes an outer box made of a metal, an inner box made of a metal, a heat insulator disposed between the outer box and the inner box and on an inner side of the outer box, an air layer disposed on an inner side of the heat insulator, and heaters disposed on the outer side of the inner box and on the inner side of the heat insulating door, for heating the inside of the culture vessel, and whereina ...

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Номер записи: 66
11-04-2013 дата публикации

TEMPERATURE CONTROLLING SURFACES AND SUPPORT STRUCTURES

Номер: US20130089925A1
Автор: CLAPP KENNETH P., Crowell Joseph D., DAMREN RICHARD L., ERDENBERGER THOMAS, Galliher Parrish M., MITCHELL PETER A., Tuohey Colin R.
Принадлежит: Xcellerex, Inc.

A heat exchange module for use in a chemical, pharmaceutical or biological reactor system can include a body configured to be disposed in the reactor system having an inner replaceable reactant container is disclosed. The body can further include at least one thermally conductive surface adapted to contact the inner replaceable reactant container to facilitate heat transfer. Furthermore, the heat exchange module can include a heat exchanger disposed within the module body and can include a fluid circulation path through which a heat exchange fluid can be circulated. 1. A heat exchange module for use in a chemical , pharmaceutical or biological reactor system , comprising:a body configured to be disposed in a reactor vessel comprising a support structure, the reactor vessel having an inner replaceable or single use reactant container, the body comprising at least one surface configured to conform to a shape of the reactor vessel and at least one thermally conductive surface adapted to contact the inner replaceable container to facilitate heat transfer, anda heat exchanger disposed within the module body comprising a fluid circulation path through which a heat exchange fluid can be circulated.2. The heat exchange module of wherein the body is an elongate body having a top end and a bottom end and is adapted to be inserted into the reactor vessel such that the body extends at least a substantial portion of the distance between a top and a bottom of the reactor vessel.3. The heat exchange module of wherein the body further comprises a coupler for attaching the module to the reactor vessel.4. The heat exchange module of wherein the coupler is a bracket or hook for attaching the module to a top portion of the reactor vessel.5. The heat exchange module of wherein the fluid circulation path comprises:an elongate tube axially positioned within the body and extending substantially the length of the body, the elongate tube configured for delivering the heat exchange fluid ...

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Номер записи: 67
18-04-2013 дата публикации

Apparatus and method for using ultrasonic radiation for controlled fragmentation of chains of nucleic acids

Номер: US20130092524A1
Автор: Babur Hadimioglu, Kapil Dev, Smriti Sharma, Vibhu Vivek
Принадлежит: Microsonic Systems Inc

Example methods and systems are directed to controlled fragmentation of genetic samples that include chains of nucleic acid. Waveform inputs to a transducer configured as Fresnel Annular Sector Actuator (FASA) are used to focus acoustic energy at the genetic sample in a controlled fragmentation process that reduces the genetic sample to a desired average fragment size for the resulting chains of nucleic acid.

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Номер записи: 68
18-04-2013 дата публикации

APPARATUS AND METHOD FOR DISPENSING CELLS OR PARTICLES CONFINED IN A FREE FLYING DROPLET

Номер: US20130095469A1
Автор: Koltay Peter, Yusof Azmi
Принадлежит: Albert-Ludwigs-Universitaet Freiburg

An apparatus for dispensing one or more cells or particles confined in a free flying droplet has a droplet generating device configured to eject out of an orifice a free flying droplet of a suspension of cells or particles. The droplet generating device has a branch-less one-way channel having the orifice at one end thereof. A device for detecting information on cells or particles located in an observation volume of the suspension within the branch-less one-way channel is provided. An ejected droplet is directed to a first position or a second position depending on the detected information. 1. An apparatus for dispensing one or more cells or particles confined in a free flying droplet , comprising:a piezo driven on-demand droplet generating device configured to eject out of an orifice a free flying droplet of a suspension of cells or particles, said droplet generating device comprising a branch-less one-way channel comprising the orifice opening at one end thereof;a device for detecting information on cells or particles located in an observation volume of the suspension within the branch-less one-way channel; anddirector for directing an ejected droplet to a first position or a second position depending on the detected information.2. The apparatus of claim 1 , wherein the observation volume is a volume of the suspension which is located adjacent to the orifice and which is ejected as a free flying droplet upon the next dispensing event.3. The apparatus of claim 1 , wherein the first position is a target position and the second position is a waste position.4. The apparatus of claim 1 , wherein the information on cells or particles comprise at least one of a presence claim 1 , a number claim 1 , a size claim 1 , a color and/or an impedance of the cells or particles.5. The apparatus of claim 1 , wherein said directors for directing are configured to direct an ejected droplet to the first position if the detected information indicates that the ejected droplet will ...

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Номер записи: 69
18-04-2013 дата публикации

CARBON DIOXIDE CAPTURE BOX

Номер: US20130095536A1
Автор: Sahoo Dinabandhu
Принадлежит:

The present subject matter relates to a system comprising a first unit that generates a polluted air comprising carbon dioxide, and a second unit mounted on the first unit. The second unit comprises a solution comprising at least one alga suspended in a solvent to purify the polluted air. The solution comprising the at least one alga is agitated upon operation of the first unit. The system further comprises a channel to guide the polluted air from the first unit to the second unit. 131-. (canceled)32. A system comprising:a first unit that generates a polluted air comprising carbon dioxide; 'a solution comprising at least one alga suspended in a solvent to purify the polluted air, wherein the solution comprising the at least one alga is agitated upon operation of the first unit; and', 'a second unit mounted on the first unit, wherein the second unit comprisesa channel to guide the polluted air from the first unit to the second unit.33. The system as claimed in claim 32 , wherein the second unit comprises at least one purifying unit partially filled with the solution comprising the at least one alga.34. The system as claimed in claim 33 , wherein the purifying unit comprises a first inlet connected to a first end of the channel to receive the polluted air from the first unit.35. The system as claimed in claim 33 , wherein the purifying unit comprises a second inlet to receive a polluted air comprising carbon dioxide from atmosphere.36. The system as claimed in claim 33 , wherein the purifying unit comprises:a first outlet to discharge at least one from a purified air and vapour generated in the purifying unit; anda second outlet to discharge the solution.37. The system as claimed in claim 33 , the purifying unit comprises at least one surface made of a transparent material to provide sunlight to the at least one alga.38. The system as claimed in claim 33 , wherein the purifying unit is made of a material selected from the group consisting of acrylic claim 33 , ...

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Номер записи: 70
18-04-2013 дата публикации

CELL SEPARATING APPARATUS AND CELL SEPARATING METHOD

Номер: US20130095564A1
Автор: YOSHIOKA Satomi
Принадлежит: SEIKO EPSON CORPORATION

A cancer cell separating apparatus includes: a flow channel including an antibody fixation area having antibodies which bind specifically to cancer cells fixed thereon, wherein the cancer cells and non-cancer cells are separated using a difference in velocity of movement between the cancer cells and the non-cancer cells in cell slurry introduced into the flow channel. 1. A cancer cell separating apparatus comprising:a flow channel including an antibody fixation area having antibodies which bind specifically to cancer cells fixed thereon,wherein the cancer cells and non-cancer cells are separated using a difference in velocity of movement between the cancer cells and the non-cancer cells in cell slurry introduced into the flow channel.2. The cancer cell separating apparatus according to claim 1 , wherein the antibody fixation area is provided on an entire surface of the flow channel.3. The cancer cell separating apparatus according to claim 1 , wherein the flow channel includes:a first portion; anda second portion and a third portion bifurcated from an one end portion of the first portion,wherein an antibody fixation prohibited area having no antibody fixed thereon is provided on the surface of the first portion on the side of the second portion, andthe antibody fixation area is provided on the surface of the first portion on the side of the third portion.4. The cancer cell separating apparatus according to claim 1 , further comprising:a first electrode pair and a second electrode pair, whereintime lengths required for the cancer cells and the non-cancer cells to pass from the first electrode pair to the second electrode pair are detected.5. The cancer cell separating apparatus according to claim 4 , further comprising a dielectric migration electrode pair.6. The cancer cell separating apparatus according to claim 5 , wherein the dielectric migration electrode pair is controlled on the basis of the time length.7. A cancer cell separating method comprising:introducing ...

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Номер записи: 71
25-04-2013 дата публикации

Fluid mixing systems with adjustable mixing element

Номер: US20130101982A1
Автор: Michael E. Goodwin, Nephi D. Jones
Принадлежит: Hyclone Laboratories LLC

A fluid mixing system includes a support housing having an interior surface bounding a chamber. A flexible bag is disposed within the chamber of the support housing, the flexible bag having an interior surface bounding a compartment. An impeller is disposed within the chamber of the flexible bag. A drive shaft is coupled with the impeller such that rotation of the drive shaft facilitates rotation of the impeller. A drive motor assembly is coupled with the draft shaft and is adapted to rotate the drive shaft. An adjustable arm assembly is coupled with the drive motor assembly and is adapted to move the drive motor assembly which in turn moves the position of the drive shaft and impeller. An electrical controller can control movement of the adjustable arm.

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Номер записи: 72
25-04-2013 дата публикации

OIL-BASED MATERIAL-PRODUCING METHOD AND OIL-BASED MATERIAL-PRODUCING APPARATUS

Номер: US20130102047A1
Автор: ISHIZUKA Akinori, Tsukahara Yasunori, YOSHINO Iwao
Принадлежит:

An oil-based material-producing method includes a microwave irradiation step of irradiating oil-based material-producing microorganisms with microwaves. The oil-based material-producing method may also include a collecting step of collecting an oil-based material produced by the oil-based material-producing microorganisms after the microwave irradiation step. 1. An oil-based material-producing method , comprising a microwave irradiation step of irradiating oil-based material-producing microorganisms with microwaves.2. The oil-based material-producing method according to claim 1 , wherein claim 1 , in the microwave irradiation step claim 1 , microwaves are irradiated in a presence of a microwave responsive material claim 1 , which is either one of a microwave-absorbing material and a microwave-sensitive material.3. The oil-based material-producing method according to claim 2 , wherein the microwave responsive material is able to flow.4. The oil-based material-producing method according to claim 3 , wherein the microwave responsive material has a shape for collecting electric field of microwaves.5. The oil-based material-producing method according to claim 2 , wherein the microwave responsive material is immobilized.6. The oil-based material-producing method according to claim 2 , wherein the microwave responsive material is at least any one of a dielectric claim 2 , a conductive substance claim 2 , and a magnetic substance.7. The oil-based material-producing method according to claim 1 , further comprising a collecting step of collecting an oil-based material produced by the oil-based material-producing microorganisms after the microwave irradiation step.8. The oil-based material-producing method according to claim 1 , wherein the oil-based material-producing microorganisms are oil-based material-producing microalgae.9. The oil-based material-producing method according to claim 1 , wherein claim 1 , in the microwave irradiation step claim 1 , microwaves having two or ...

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Номер записи: 73
25-04-2013 дата публикации

Cell monitoring by means of scattered light measurement

Номер: US20130102067A1
Автор: Kriemhilt Roppert, Oliver Hayden, Peter Ertl, Sandro Francesco Tedde
Принадлежит: SIEMENS AG

A device for monitoring test cells has at least one receiving unit for the test cells and a first measuring unit for cell measurement. With a second measuring unit, which has a light source and a scattered light detector, cell monitoring can be carried out during the cell measurement. For this purpose the receiving unit has an at least partially light-permeable substrate and is arranged between the light source and scattered light detector such that at least a part of the light generated by the light source shines on the receiving unit, is scattered on the test cells and, after leaving the receiving unit through the substrate, impinges on the scattered light detector.

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Номер записи: 74
02-05-2013 дата публикации

Continuous flow micro-crusher

Номер: US20130104362A1
Автор: Joanna Aizenberg, Joseph Ashley Taylor, Paul Kolodner, Thomas N. Krupenkin
Принадлежит: Lucent Technologies Inc

The present invention provides an apparatus comprising a substrate and first and second disks. The disks are rotatably located over the substrate, each disk having an outer circumference with teeth thereon. The first disk is positioned to interleave one or more of its teeth with the teeth of the second disk. The substrate includes a channel with an exit port located near the teeth of one of the disks. Another apparatus comprises at least one disk rotatably located over a substrate and in a well of the substrate, the disk having an outer circumference with teeth thereon. The disk is positioned to provide a maximum distance of less than about 10 microns between each one the teeth and a nearest wall defining the well. The substrate includes a channel with an exit port located near the teeth of the disk.

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Номер записи: 75
02-05-2013 дата публикации

Method and apparatus for growing photosynthetic organisms

Номер: US20130109008A1
Автор: Arnold Mangott, Howard Kneebone, Kirsten Heimann, Marc Rene Stammbach, Rocky De Nys
Принадлежит: MBD Energy Ltd

A biological cultivation system for the culture of photosynthetic organisms including at least one cultivation chamber permitting exposure of the culture medium to natural and/or artificial light and including; a light transmissive wall or walls defining a gas space; and a culture medium containment area below the gas space; one or more fluid inlets positioned within the culture medium containment area; and one or more gas outlets in communication with the gas space; a control unit operatively connected to a gas flow control device, the gas flow control device controlling the flow of gas in through the fluid inlets and out through the fluid outlets to control the conditions within the cultivation chamber.

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Номер записи: 76
02-05-2013 дата публикации

Methods of and devices for capturing circulating tumor cells

Номер: US20130109011A1
Автор: Daniel Sang-won PARK, Dimitris E. Nikitopoulos, Michael C. Murphy, Sudheer D. Rani, Taehyun Park
Принадлежит: Louisiana State University and Agricultural and Mechanical College

A device and methods are provided for efficient and quick capture of target cells through a main microchannel having capture elements immobilized thereon and manipulating a velocity profile of a sample as it passes through the main microchannel. The cell capture device may have a main microchannel with a depth slightly larger than the diameter of the target cells and a plurality of side microchannels. The side microchannels may have a depth smaller than the diameter of the target cells. The device and methods may be used for early cancer detection.

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Номер записи: 77
02-05-2013 дата публикации

CULTURE APPARATUS FOR MICROSCOPE VIEWING AND METHOD THEREFOR

Номер: US20130109081A1
Автор: Tsuchiya Hideharu
Принадлежит: TOKAI HIT CO., LTD.

A culture apparatus for microscopic viewing enables effective microscopic viewing at high magnification of samples such as cells suspended in a culture solution while the samples are maintained at a uniform temperature. The apparatus is used to microscopically view a sample placed on a microscope stage, and is provided with a housing unit that houses the vessel containing a culture solution and the sample; a lid that closes the aperture on the upper surface side of the housing unit; a transparent sheet top heater provided in the portion of the lid that corresponds to the viewing area; and a temperature detecting means where a thin wire detection unit penetrates into the interiors of the housing unit and a well plate to directly measure the temperature of the culture solution. Based on the temperature information, a controller controls the temperature of the transparent sheet top heater using a feedback method. 1. A culture apparatus for microscope viewing enabling effective microscopic viewing a specimen placed on a stage of the microscope including a housing unit for accommodating a container holding a culture solution including the specimen therein , and a lid for closing an upper opening of the housing unit , with the housing unit having a lower opening of a size into which is removably fit a well plate and no heating plate narrowing the lower opening comprising:a transparent sheet top heater provided over a portion of the lid that corresponds to the viewing area; anda temperature detecting means in which a thin wire detector penetrates into the interior of the housing unit and further is immersed in the culture solution hold in a container to measure the temperature of the culture solution supplied thereto directly;wherein the temperature of the transparent sheet top heater is controlled through a feedback method on the basis of the temperature information obtained from the temperature detecting means.2. The culture apparatus for microscope viewing according to ...

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Номер записи: 78
02-05-2013 дата публикации

Closed Photobioreactor System For Continued Daily In Situ Production Of Ethanol From Genetically Enhanced Photosynthetic Organisms With Means For Separation And Removal Of Ethanol

Номер: US20130109084A1
Автор: Benjamin Moll, Edward Legere, Edwin Malkiel, Robert Paul Woods
Принадлежит: Algenol Biofuels Switzerland GmbH

The invention provides a device for growing genetically enhanced aquatic photoautotrophic organisms in a stable culture, causing the organisms to produce ethanol, and then separating, collecting, and removing the ethanol in situ.

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Номер записи: 79
02-05-2013 дата публикации

Closed Photobioreactor System For Continued Daily In Situ Production Of Ethanol From Genetically Enhanced Photosynthetic Organisms With Means For Separation And Removal Of Ethanol

Номер: US20130109085A1
Автор: Benjamin Moll, Edward Legere, Edwin Malkiel, Robert Paul Woods
Принадлежит: Algenol Biofuels Switzerland GmbH

The invention provides a device for growing genetically enhanced aquatic photoautotrophic organisms in a stable culture, causing the organisms to produce ethanol, and then separating, collecting, and removing the ethanol in situ.

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Номер записи: 80
09-05-2013 дата публикации

Container and cap for a biological specimen

Номер: US20130112016A1
Автор: Timothy R. Hansen
Принадлежит: TriPath Imaging Inc

A container for collecting a biological specimen is provided that includes a peripheral wall extending from a base. The container further includes a longitudinal tower disposed within the container. The tower forms a partition within the container to form at least a first chamber and a second chamber therein, wherein the first and second chambers are in fluid communication with one another. In addition, the container include an insert disposed within the container opposite the base, the insert comprising at least a first opening and a second opening. The second opening provides access to the second chamber and is further configured to engage a specimen collecting device to leave a portion of the specimen collecting device within the second chamber. The first opening provides access to the first chamber and is further configured to receive a syringe therein for accessing the biological specimen. Associated systems and methods are also provided.

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Номер записи: 81
09-05-2013 дата публикации

INTEGRATED BIOREACTOR AND SEPARATION SYSTEM AND METHODS OF USE THEROF

Номер: US20130115588A1
Автор: Benson Todd, Davis Kim, Guenev Guenko, Kacmar James, Marconi Derrick, Moll John
Принадлежит:

The present invention relates to methods and systems for culturing and purifying target substance(s), such as selected proteins, viruses, pathogenic bacteria, antibodies, antigens, clotting factors, glycoproteins, and hormones, from source liquids wherein the culturing and purification is effected in an integrated system including a bioreactor and at least one separation unit that functions under the control of a single operating system and provides for disposable components. 1. A culturing and separating system comprising:a disposable container for housing biomaterials for processing, wherein the disposable container is held in support structure;a separating unit in fluid communication with the disposable container, wherein the separating unit comprises at least one disposable filtration unit; anda monitoring means for operating and controlling conditions in the bioreactor and separation unit.2. The system according to claim 1 , wherein the disposable contain further comprises at least one input port claim 1 , at least one exhaust port claim 1 , at least one harvest port claim 1 , and one or more sensors for sensing one or more parameters of the biomaterials in the disposable container.3. The system according to claim 2 , wherein the parameters are temperature claim 2 , dissolved oxygen claim 2 , pH claim 2 , tank level claim 2 , agitation speed claim 2 , levels of nutrients claim 2 , flow rate of gas or fluids claim 2 , inlet and outlet pressure claim 2 , conductivity claim 2 , turbidity claim 2 , or UV radiation.4. The system according to claim 1 , wherein the disposable container hold from about 5 to 5000 liters of medium.5. The system according to claim 1 , wherein the disposable container is a liner within the support structure or bag.6. The system according to claim 5 , wherein the liner or bag is fabricated from a polymeric or cellulose containing material.7. The system according to claim 1 , wherein the disposable filtration unit comprises at least one ...

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Номер записи: 82
16-05-2013 дата публикации

CULTURE VESSEL FOR FORMING AGGREGATED CELL MASS

Номер: US20130122580A1
Автор: Tsukada Ryouhei, YOKOYAMA Kanehisa
Принадлежит: SUMITOMO BAKELITE CO., LTD.

The present invention provides a culture vessel for forming an aggregated cell mass which has at least two wells, wherein the wells are made from a light-shielding member and an inner surface of the wells is subjected to treatment to impart cellular non-adhesiveness. 1. A culture vessel for forming an aggregated cell mass , comprising:a light-shielding member which has at least two wells, wherein an inner surface of the at least two wells is subjected to treatment to impart cellular non-adhesiveness.2. The culture vessel for forming an aggregated cell mass according to claim 1 , wherein the light-shielding member comprises a colored resin.3. The culture vessel for forming an aggregated cell mass according to claim 2 , wherein the colored resin is a white resin.4. The culture vessel for forming an aggregated cell mass according to claim 1 , wherein the treatment to impart cellar non-adhesiveness for forming an aggregated cell mass is hydrophilic treatment of the surface.6. The culture vessel for forming an aggregated cell mass according to claim 1 , wherein the wells have a portion in which the diameter of the inner surface of the side wall reduces toward the bottom inner surface.7. The culture vessel for forming an aggregated cell mass according to claim 6 , wherein the portion in which the diameter reduces is conical or hemispheric.8. The culture vessel for forming an aggregated cell mass according to claim 4 , wherein the well has a bottom part with a longitudinal section having an approximate U-shape and an approximate circular opening claim 4 , at least a curved surface portion of the bottom inner surface is provided with a hydrophilic resin layer and the curvature radius (R) of the bottom inner surface is equal to or more than 1.0 mm and equal to or less than 3.0 mm.9. The culture vessel for forming an aggregated cell mass according to claim 2 , wherein the treatment to impart cellar non-adhesiveness for forming an aggregated cell mass is hydrophilic treatment ...

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Номер записи: 83
23-05-2013 дата публикации

Purification Methods and Systems Related to Renewable Materials and Biofuels Production

Номер: US20130130335A1
Автор: Austin Glen, Bhattacharjee Binita X., Bolton Leslie W., Borden Jacob, Carrera Martin E., Gokhale Amit A., Horler Chris, Hurley Aidan, Mack Eric T.
Принадлежит: BP CORPORATION NORTH AMERICA INC.

Methods of producing renewable materials may include consuming a fermentation feedstock with a fermentation organism to produce a renewable material in fermentation broth; water may then be separated from the feedstock or broth using one or more phase separations, or the renewable material may be concentrated from the feedstock or broth using one or more phase separations. Methods of producing biofuel components may include consuming a lignocellulosic or sugar fermentation feedstock with a fermentation organism to produce either ethanol or butanol in fermentation broth; cooling the feedstock or broth to solidify at least some water therein; and separating the solidified water from the feedstock or broth using a solid-liquid phase separation. 1. A method of producing a renewable material , the method comprising:consuming at least a portion of a fermentation feedstock with a fermentation organism to produce a renewable material in fermentation broth, the fermentation feedstock and fermentation broth comprising water; andseparating at least a portion of the water from the fermentation feedstock or fermentation broth using one or more phase separations.2. The method of claim 1 , wherein the step of separating comprises a solid-liquid separation.3. The method of claim 1 , wherein the step of separating comprises a liquid-liquid separation.4. The method of claim 1 , wherein the step of separating comprises a solid-liquid separation and a liquid-liquid separation.5. The method of claim 4 , further comprising removing enthalpy from the fermentation feedstock and/or fermentation broth.6. The method of claim 1 , wherein the step of removing enthalpy comprises an endothermic reaction.7. The method of claim 1 , further comprising lowering a temperature of the fermentation feedstock and/or the fermentation broth below an incipient crystallization temperature.8. The method of claim 7 , comprising using fluidized bed heat exchangers for crystallization.9. The method of claim 1 , ...

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Номер записи: 84
30-05-2013 дата публикации

System and method including analytical units

Номер: US20130137110A1
Автор: Charles S. Kraihanzel
Принадлежит: Beckman Coulter Inc

Systems and methods for processing and analyzing samples are disclosed. The system may process samples, such as biological fluids, using assay cartridges which can be processed at different processing locations. In some cases, the system can be used for PCR processing. The different processing locations may include a preparation location where samples can be prepared and an analysis location where samples can be analyzed. To assist with the preparation of samples, the system may also include a number of processing stations which may include processing lanes. During the analysis of samples, in some cases, thermal cycler modules and an appropriate optical detection system can be used to detect the presence or absence of certain nucleic acid sequences in the samples. The system can be used to accurately and rapidly process samples.

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Номер записи: 85
30-05-2013 дата публикации

SYSTEM AND METHOD INCLUDING ANALYTICAL UNITS

Номер: US20130137164A1
Автор: ALARURI Sami D., DAVIS Matthew S., ERICKSON Matthew D., JOHNSON Alan N., MAURER Garrick A., Sauerburger Mark F., SCHMIDT Daniel R., STACHELEK Thomas M., Wilson Brian D., WILTSIE Joshua D., YANG David L.
Принадлежит: Beckman Coulter, Inc.

Systems and methods for processing and analyzing samples are disclosed. The system may process samples, such as biological fluids, using assay cartridges which can be processed at different processing locations. In some cases, the system can be used for PCR processing. The different processing locations may include a preparation location where samples can be prepared and an analysis location where samples can be analyzed. To assist with the preparation of samples, the system may also include a number of processing stations which may include processing lanes. During the analysis of samples, in some cases, thermal cycler modules and an appropriate optical detection system can be used to detect the presence or absence of certain nucleic acid sequences in the samples. The system can be used to accurately and rapidly process samples. 1156.-. (canceled)157. A thermal cycler module for performing real time PCR within a PCR reaction vessel , comprising:a thermal block comprising a receptacle for receiving a PCR reaction vessel;a movable lid, the lid overlapping the thermal block and having an open position and a closed position, the movable lid capable of moving between the open and closed positions;an excitation optics assembly, the excitation optics assembly configured to pass excitation light to the PCR reaction vessel when the PCR reaction vessel is located in the receptacle; andan emission optics assembly, the emission optics assembly is configured to receive light from the PCR reaction vessel when the PCR reaction vessel is located in the receptacle in the thermal block,wherein the thermal cycler module is configured to fit in one of a plurality of slots in a thermal cycler garage.158. The thermal cycler module of claim 157 , wherein the receptacle of the thermal block is cooperatively configured with the PCR reaction vessel and is in thermal contact with the PCR reaction vessel when the PCR reaction vessel is in the receptacle claim 157 , and wherein the receptacle ...

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Номер записи: 86
06-06-2013 дата публикации

SEPARATIVE HARVESTING DEVICE

Номер: US20130143313A1
Автор: Niazi Sarfaraz K.
Принадлежит: Therapeutic Proteins International, LLC

A harvesting device for capturing a biological product directly by binding the secreted biological product with a resin, discarding the nutrient medium and eluting the biological product as a concentrated solution, eliminating the steps of sterile filtration and volume reduction, thus allowing one to combine the steps of recombinant expression and separation of a biological product. The method allows loading of resin for column-purification, eliminating all steps of perfusion process and maintaining a sink condition of a toxic product in nutrient medium to optimize productivity of host cells. The instant invention also allows harvesting of solubilized inclusion bodies after the cells have been lysed and refolding of proteins inside the bioreactor. 1. A harvesting device for capturing a biological product comprising at least one container with at least one external surface and an inner volume to hold at least one ligand or resin capable of binding a biological product and the surface having a plurality of pores.2. The harvesting device according to claim 1 , wherein the container is a flexible pouch.3. The harvesting device according to claim 1 , wherein the container comprises a plastic claim 1 , nylon claim 1 , metallic mesh claim 1 , or a composite material.4. The harvesting device according to claim 1 , wherein the size of pores is between 0.2 and 30 microns.5. The harvesting device according to claim 1 , wherein the size of pores is between 1 and 5 microns.6. The harvesting device according to claim 1 , wherein the resin or ligand is an ionic-exchange resin claim 1 , a hydrophobic resin claim 1 , a stimuli responsive polymer claim 1 , an affinity resin claim 1 , Protein A-coated magnetic beads claim 1 , Protein-G magnetic beads claim 1 , a dual affinity polypeptide claim 1 , Q Hypercel sorbant claim 1 , S Hypercel sorbent claim 1 , 4-mercaptoethylpyridine (MEP) claim 1 , Protein G claim 1 , countercurrent chromatography media claim 1 , a peptide ligand claim 1 , ...

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Номер записи: 87
06-06-2013 дата публикации

SHEET-SHAPED CELL CULTURE DISSOCIATION SYSTEM AND METHOD

Номер: US20130143315A1
Автор: Yamamoto Takashi
Принадлежит:

Disclosed is a system for dissociating a sheet-shaped cell culture into individual cells. The system, so configured as to minimize the amount of damage to cells when dissociating a sheet-shaped cell culture into individual cells, in one form includes: (i) a reaction unit that dissociates the sheet-shaped cell culture; (ii) a sensor unit that acquires information relating to a particle size distribution of cells inside the reaction unit; and (iii) an analysis unit that computes the particle size distribution of the cells from the information acquired by the sensor unit and determines and outputs a dissociation state. 1. A system for dissociating a sheet-shaped cell culture into individual cells , comprising:(i) a reaction unit which dissociates the sheet-shaped cell culture;(ii) a sensor unit which acquires information about a particle size distribution of cells existing in the reaction unit; and(iii) an analysis unit which calculates the particle size distribution of cells from the information acquired by the sensor unit, thereby determining and outputting a state of dissociation.2. The system as defined in claim 1 , wherein the sheet-shaped cell culture contains a skeletal myoblast.3. The system as defined in claim 1 , further comprising a reaction control unit which controls the dissociation of cells in the reaction unit.4. The system as defined in claim 2 , further comprising a reaction control unit which controls the dissociation of cells in the reaction unit.5. The system as defined in claim 3 , wherein the reaction control unit includes at least one of an environment control unit claim 3 , a chemical action control unit claim 3 , and a mechanical action control unit.6. The system as defined in claim 4 , wherein the reaction control unit includes at least one of an environment control unit claim 4 , a chemical action control unit claim 4 , and a mechanical action control unit7. A system for determining a state of dissociation of a sheet-shaped cell culture in a ...

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Номер записи: 88
13-06-2013 дата публикации

Use of oxyhydrogen microorganisms for non-photosynthetic carbon capture and conversion of inorganic and/or c1 carbon sources into useful organic compounds

Номер: US20130149755A1
Автор: John S. Reed, Lisa Dyson
Принадлежит: Kiverdi Inc

Compositions and methods for a hybrid biological and chemical process that captures and converts carbon dioxide and/or other forms of inorganic carbon and/or CI carbon sources including but not limited to carbon monoxide, methane, methanol, formate, or formic acid, and/or mixtures containing CI chemicals including but not limited to various syngas compositions, into organic chemicals including bio-fuels or other valuable biomass, chemical, industrial, or pharmaceutical products are provided. The present invention, in certain embodiments, fixes inorganic carbon or CI carbon sources into longer carbon chain organic chemicals by utilizing microorganisms capable of performing the oxyhydrogen reaction and the autotrophic fixation of CO 2 in one or more steps of the process.

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Номер записи: 89
13-06-2013 дата публикации

Systems and methods for high speed array printing and hybridization

Номер: US20130150266A1
Автор: Holger Eickhoff, Thomas C. Tisone
Принадлежит: Biodot Inc

Novel and improved systems and methods for high speed arraying, hybridization, quantitative development and/or assaying are provided. Some embodiments provide a web based arraying format. Some other embodiments provide a sheet based arraying format. Some embodiments use a drop on drop assaying or hybridization mode. In some embodiments, a substantially inert substrate is utilized. In some other embodiments, an interactive substrate is utilized.

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Номер записи: 90
20-06-2013 дата публикации

System and process for correcting constant volume acidity of fermentative media for producing organic acids

Номер: US20130157329A1
Автор: Paulo Yoshida
Принадлежит: COMPANHIA REFINADORA DA AMAZONIA

A system ( 100, 200 ) is described for correcting constant volume acidity of fermentative media for the production of organic acids, which comprises a fermenter ( 1 ) provided with a pH sensor (P 1 ) a filtration module ( 2 ) provided with a filtering element, an addition vessel ( 3 ) provided with a main metering element ( 4 ), and a heat exchanger ( 5 ). Operation of the system ( 100, 200 ) is controlled by a controller (CT). As soon as the sensor (P 1 ) detects a reduction in pH to below the ideal values for producing organic acid, the controller (CT) calculates the amount of fermentative medium to be withdrawn from the fermenter ( 1 ) and said amount is conveyed to the vessel ( 3 ) in order to be alkalinized and returned to the system. Arrangements of the system with more than one fermenter are also described. The process for correcting acidity used by the system ( 100, 200 ) of the invention is likewise described.

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Номер записи: 91
20-06-2013 дата публикации

Cell-culture-bag

Номер: US20130157353A1
Автор: Elise Leonore Isolde Dijkhuizen Borgart, Joost Dick De Bruijn, Madelon Sophia George Maria Bracke
Принадлежит: XPAND BIOTECHNOLOGY BV

A cell-culture-bag for use in the expansion of stem cells from a crude biopsy, comprising: outer walls; a chamber located within the walls; a first inlet, and first and second outlets, providing fluid communication with the chamber, for connection to a perfusion apparatus; and a filter arrangement constructed to allow passage of red blood cells and block passage of stem cells from the first outlet, and block passage of microcarriers from the second outlet.

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Номер записи: 92
27-06-2013 дата публикации

Imaging and evaluating embryos, oocytes, and stem cells

Номер: US20130162795A1
Автор: Barry Behr, Connie C. Wong, Kevin E. Loewke, Renee A. Reijo-Pera, Thomas M. Baer
Принадлежит: Leland Stanford Junior University

Methods, compositions and kits for determining the developmental potential of one or more embryos or pluripotent cells and/or the presence of chromosomal abnormalities in one or more embryos or pluripotent cells are provided. These methods, compositions and kits find use in identifying embryos and oocytes in vitro that are most useful in treating infertility in humans.

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Номер записи: 93
04-07-2013 дата публикации

Method and device for isolating cells from heterogeneous solution using microfluidic trapping vortices

Номер: US20130171628A1
Автор: Albert J. Mach, Dino Di Carlo, Soojung C. Hur
Принадлежит: UNIVERSITY OF CALIFORNIA

A method of isolating cells includes providing a microfluidic device having at least one microfluidic channel coupled to an inlet and an outlet, the at least one microfluidic channel comprises at least one expansion region disposed along the length thereof. The at least one expansion region is an abrupt increase in a cross-sectional dimension of the at least one microfluidic channel configured to generate a vortex within the at least one expansion region in response to fluid flow. A solution containing a population of cells at least some of which have diameters ≧10 μm flows into the inlet. A portion of cells is trapped within vortex created within the at least one expansion region. The trapped cells may then released from the expansion region.

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Номер записи: 94
11-07-2013 дата публикации

THERMAL CYCLER FOR PCR INCLUDING TEMPERATURE CONTROL BLADDER

Номер: US20130177945A1
Автор: BELGRADER Phillip
Принадлежит:

Methods and devices for performing chemical reactions under controlled temperature are described. In one embodiment, the devices provided by the invention comprise a housing diminished hold a reaction chamber disposed within an interior volume of the housing. The reaction chamber has thermally conductive interior and exterior surfaces defining an internal volume therein at a first temperature. The device also includes at least one temperature-control substance at a second temperature into said bladder and expel said temperature-control substance, the bladder expands to about substantially at least a portion of said exterior surfaces of said reaction chamber to enable thermal exchange between said temperature-control substance the said internal volume of reaction chamber. 112-. (canceled)13. A method for performing a polymerase chain reaction (PCR) , comprising:providing a reaction chamber having thermally conductive interior and exterior surfaces defining an internal volume within said reaction chamber, said interior volume being at a first temperature;providing a pair of thermally conductive temperature-control bladders said pair of bladders being positioned in a substantially opposing arrangement with respect to each other and being configured to receive a temperature-control substance at a second temperature into said pair of bladders and expel said temperature-control substance from said pair of bladders; and wherein said pair of bladders being further configured such that upon receiving said temperature-control substance said pair of bladders expand to abut substantially at least a portion of said exterior surfaces of said reaction chamber to enable thermal exchange between said temperature-control substance and said internal volume of reaction chamber;introducing said temperature-control substance under conditions effective to cause an expansion of said pair of bladders to bring said pair of bladders into substantially abutting said at least a portion of said ...

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Номер записи: 95
11-07-2013 дата публикации

Sulfide generation process and system for syngas fermentation

Номер: US20130177957A1
Автор: Jianxin Du, Rathin Datta
Принадлежит: Coskata Inc

A process that generates sulfide in production of liquid products from syngas and a system for syngas fermentation including a sulfide generation process to convert feed gas to liquid product, the process including: partially oxidizing high-temperature gas, input gas, and molten sulfur to generate sulfide gas, the high-temperature gas containing oxygen, the input gas containing carbonaceous fuel and the sulfide gas containing hydrogen sulfide, CO, CO 2 , and H 2 ; mixing the sulfide gas with the feed gas to generate mixed gas having a predetermined hydrogen sulfide concentration; providing at least a portion of the mixed gas to a production fermentor containing microorganisms; and converting the mixed gas to the liquid product by contact with the microorganisms.

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Номер записи: 96
18-07-2013 дата публикации

Method and Apparatus for Producing Engineered Fuel from High Cellulose Feedstock

Номер: US20130183715A1
Автор: Paul T. Baskis
Принадлежит: Individual

An apparatus and method for producing methane gas, synthetic hydrocarbon gas, and fertilizer is provided. The apparatus includes a mix tank for mixing cellulosic material with a solvent into a slurry and a generator having an exhaust. The apparatus further includes a stir tank reactor for converting the slurry to a solution containing lignin-like carbon and liquid, and a separator for separating the lignin-like carbon and liquid. An anaerobic digester decomposes the received liquid received from the stir tank into methane and liquid components. A carbon dioxide scrubber scrubs the methane component of carbon dioxide. The method includes mixing cellulosic material with a solvent into a slurry, and converting the slurry to a solution containing lignin-like carbon and liquid. It also includes separating the lignin-like carbon and liquid and decomposing the liquid into methane and liquid components, and scrubbing the methane component of carbon dioxide.

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Номер записи: 97
18-07-2013 дата публикации

METHOD FOR HARVESTING EXPRESSION PRODUCTS

Номер: US20130183742A1
Автор: Leschke Christian, Wehnes Engelbert, Werner Uwe
Принадлежит: BAVARIAN NORDIC A/S

The present invention provides a method for recovering an essentially cell-associated expression product from a host cell comprising (a) culturing said host cell under conditions that allow expression of said expression product; (b) collecting said host cell in/on a filter unit; (c) disrupting said host cell in/on the filter unit; and (d) separating said expression product from said disrupted host cell. Said host cell is preferably a vertebrate cell, more preferably an avian cell, which is preferably cultured in suspension. Furthermore, the present invention provides for the use of a filter unit characterized in that said filter unit is (i) suitable to retain a host cell which expresses an expression product; and (ii) suitable for elution of said expression product from the filter unit after cell disruption in/on said filter unit for recovering said expression product from said host cell as well as for a system for recovering an expression product from a host cell comprising said filter unit. The present invention also provides an expression product obtainable by said method, said expression product being preferably a virus, specifically a poxvirus, in particular selected from the group consisting of fowlpoxvirus, vaccinia virus and, more preferably, modified vaccinia virus Ankara, MVA. 121-. (canceled)23. The method of claim 22 , wherein the poxvirus is selected from the group consisting of fowlpox virus claim 22 , vaccinia virus claim 22 , and modified vaccinia virus Ankara (MVA).24. The method of claim 23 , wherein the filter unit is suitable to separate the host cell from cell culture medium.25. The method of claim 23 , wherein the filter unit is suitable to allow passing through of the expression product and/or elution of the expression product from the host cell after cell disruption in or on the filter unit.26. The method of claim 25 , wherein the filter unit is further suitable to allow passing through of a disrupted host cell.27. The method of claim 23 , ...

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Номер записи: 98
18-07-2013 дата публикации

Treatment of organic waste

Номер: US20130183745A1
Автор: Jorma Oinas, Seppo Aho
Принадлежит: SAVATERRA OY

There is provided an apparatus for assisting hygienization of organic waste. The apparatus comprises at least one reception means for receiving organic solid matter and organic waste, mixing means for mixing the organic waste and the organic solid matter together into an organic mixture, selecting means for selecting whether to expose the organic mixture in the mixing means to either: aeration in order to create a homogenized and internally oxygenous organic mixture, thereby accelerating the hygienization of the organic mixture, or a heat treatment, wherein the heat treatment performs the hygienization of the organic mixture, or to neither the aeration nor the heat treatment. The mixing means may then expose the organic mixture to the aeration, or to the heat treatment, or to neither of the aeration nor the heat treatment on the basis of the selection. The apparatus may further comprise delivery means for passing the organic mixture to either aerobic biodegradation or an end user.

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Номер записи: 99
25-07-2013 дата публикации

System and Method for Processing Alternate Fuel Sources

Номер: US20130186810A1
Автор: Bart T. Lynam, John J. Sudnick, Thomas A. Volini
Принадлежит: Individual

An energy conserving wastewater treatment system capable of being fueled by alternate fuel sources comprises a synthesis gas generator that produces synthesis gas from a fuel and an organic waste digester that produces biogas. A combined synthesis gas and biogas storage reservoir that is in communication with both the synthesis gas generator and the organic waste digester. At least one boiler is in communication with the combined synthesis gas and biogas storage reservoir.

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Номер записи: 100