ФАРМАЦЕВТИЧЕСКАЯ КОМПОЗИЦИЯ, ОБЛАДАЮЩАЯ ТРОМБОЛИТИЧЕСКИМИ, ПРОТИВОВОСПАЛИТЕЛЬНЫМИ И ЦИТОПРОТЕКТИВНЫМИ СВОЙСТВАМИ

Изобретение относится к медицине, в частности к фармацевтической промышленности. Фармацевтическая композиция содержит ферментный препарат протосубтилин Г3Х, или Г10Х, или Г20Х, полиэтиленоксид с молекулярной массой 400-20000 Да, декстран с молекулярной массой 10-70 кДа и буферную смесь с рН 7,5-8,2 при определенном содержании компонентов. Изобретение позволяет получить композицию, обладающую многоцелевым синергичным действием, а также способной при лиофилизации образовывать однородную, пористую массу. 1 з.п. ф-лы, 1 табл., 4 ил.

ФИКСИРОВАННЫЙ НА НОСИТЕЛЕ ФЕРМЕНТ И СПОСОБ ЕГО ПОЛУЧЕНИЯ

Изобретение относится к области биотехнологии и может быть применено при ферментативной реакции синтеза, а также для фиксации ферментов на носителе. Фиксированный на носителе фермент выбран из группы, состоящей из пенициллин-G-амидазы (E. C.3.5.I, II), глутарил-7-АСА-ацилазы и оксидазы D-аминокислоты (E.C.I. 4.3.3), ковалентно связан на материале-носителе на основе полиорганосилоксана с функциональными аминогруппами. Способ иммобилизации заключается в ковалентном связывании фермента на материале-носителе с помощью диальдегида. В каждом конкретном случае устанавливают оптимальное соотношение фермента к материалу-носителю. Используют материал-носитель со средним диаметром частиц, преимущественно 0,01-3 и 0,1-0,4 мм, а также шарообразный. Предложенный фиксированный на носителе фермент обладает повышенной объемной активностью и стабильностью. 2 с. и 19 з.п. ф-лы, 2 табл., 5 ил.

ИМПЛАНТАЦИЯ ИНКАПСУЛИРОВАННЫХ БИОЛОГИЧЕСКИХ МАТЕРИАЛОВ ДЛЯ ЛЕЧЕНИЯ ЗАБОЛЕВАНИЙ

... 1. Состав для клеточной терапии, содержащий множество инкапсулирующих устройств, содержащих покрытие из полиэтиленгликоля (PEG) с молекулярной массой примерно от 900 и примерно до 3000 Д, а также множество клеток, инкапсулированных в инкапсулирующих устройствах, при этом указанный состав имеет плотность клеток по меньшей мере примерно 100000 клеток/мл. 2. Состав по п.1, отличающийся тем, что инкапсулирующие устройства представляют собой микрокапсулы. 3. Состав по п.2, отличающийся тем, что микрокапсулы представляют собой клеточные агрегаты с нанесенным конформным покрытием. 4. Состав по п.3, отличающийся тем, что клеточные агрегаты представляют собой панкреатические островки. 5. Состав по п.4, отличающийся тем, что плотность клеток составляет по меньшей мере 6000000 клеток/мл. 6. Состав по п.1, отличающийся тем, что клетку выбирают из группы, включающей неврологическую, сердечно-сосудистую, печеночную, эндокринную, кожную, кроветворную, иммунную, нейросекреторную, метаболическую, системную ...

KONJUGATE MIT BIOLOGISCH ABBAUBAREM POLYMER UND VERWENDUNG DAFÜR

Polymermembran mit in der Membran lokalisierten Enzymen sowie Verfahren zur Herstellung von Erzeugnissen mittels in Polymermembranen ablaufender Reaktionen

A polymer membrane (10) in whose pores are localised enzymes for biocatalytic purposes is formed by open pores (11) that extend through the membrane (10) with cylindrical to conical pores (10) in the cross-section of the membrane (10). At least the pore length (13) and/or pore cross-section (16) are adjusted depending on the type of a substrate (15) supplied to the membrane (10) and on a product (16) formed from the substrate (15) by the enzymes (12). Also disclosed is a process in which the enzyme charge density in the pores of the membrane is adjusted depending on pore diameter and pore length, so that the transverse diffusion of the substrate that flows through the pores up to the enzymes arranged in a side wall area of the pores be an adjustable parameter of the desired chain length and/or ramification of the thus obtained macromolecular product.

Antitumormittel enthaltend einen Komplex einer Oxidoreduktase und eines Polymers und ein Substrat des Enzyms

NICHT ANTIGEN WIRKENDE VERZWEIGTE POLYMERKONJUGATE

MOLEKULARE PRÄGUNGEN ZUR ERKENNUNG IN WÄSSRIGEN MEDIEN, HERSTELLUNGSVERFAHREN DAFÜR UND VERWENDUNGEN DAVON

PROTEINIC COMPOSITIONS

... 1,218,620. Proteinic compositions. ROHM & HAAS G.m.b.H. 20 Dec., 1968 [27 Dec., 1967], No. 60811/68. Headings C3H and C3P. [Also in Division A5] A water-insoluble composition comprises a water-insoluble polymer or copolymer of acrylic and/or methacrylic anhydride and a proteinic substance containing at least one - or #-amino group, the said proteinic substance being covalently bonded to the said polymer or copolymer through reactive groups on the polymer or copolymer. The proteinic substance may be an enzyme, polypeptide, antigen or antibody. The polymer or copolymer may be cross-linked either with a di- or polyamine, or may be cross-linked by copolymerizing with a monomer which contains at least two carbon-carbon double bonds. In the examples (1) polymethacrylic anhydride, cross-linked with hexamethylene diamine, is reacted in an aqueous medium with either trypsin or papain, (2) a cross-linked copolymer of methacrylic anhydride and divinyl benzene is partially saponified with ammonia and ...

MEMBRANE

PROCEDURE FOR THE PRODUCTION OF NEW EN CONNECTIONS FROM COPOLYMERS OF THE VINYLENGLY COLS AND A TO IT CHEMICAL BOUND ONE BIOLOGICAL ACTIVE SUBSTANCE

PROCEDURE FOR THE ADJUSTMENT OF BIOLOGICALLY ACTIVE PROTEIN TO PP

PROCEDURE FOR THE PRODUCTION OF STABILIZED CARRIER-BOUND PROTEINS

PROCEDURE FOR THE PRODUCTION OF BIOCATALYSTS BY IMMOBILIZATION OF ENZYMATICALLY ACTIVE ONE CELL MATERIAL

LAGERUNGSUND PRODUCTION PROCEDURE FOR a latches OF IMMOBILIZATION TABLETS

PHARMACEUTICAL COMPOSITION WITH THROMBOLYTI, ENTZÜNDUNGSHEMMENDEN ONES AND ZYTOPROTEKTIVEN CHARACTERISTICS

ENZYME PREPARATIONS

MODIFIED POLYPEPTID

POLYMER-STABILIZED PROTEINASEN

USE FROM 1 (1 - PYRROLIDINYLCARBONYL) PYRIDINIUMSALZEN TO COUPLING OF CONNECTIONS AT CARBOXYLIERTE PARTICLES AND TEST SET THIS CONTAINING

PHENYLENGRUPPEN HALTIGE ORGANOPOLYSILOXANE AND PROCEDURE FOR YOUR PRODUCTION.

NEUTRALIZATION OF HEPARIN.

IMMOBILIZATION OF BIOCHEMICAL SUBSTANCES

MACRO-POROUS, HYDROPHILIC CARRIERS FOR ENZYMES.

VERFAHREN ZUR HERSTELLUNG VON BIOKATALYSATOREN DURCH IMMOBILISIERUNG VON ENZYMATISCH AKTIVEM ZELLMATERIAL

CARRIER-FIXED PENICILLIN G AMIDASE, GLUTARYL-7 ACA ACYLASE OR D-AMINOSAÜRE-OXIDASE

PROCEDURE FOR THE IMMOBILIZATION OF THIOLVERBINDUNGEN OVER ACTIVATION OF POLYMERS, ACTIVATED POLYMERS AND IN THIS PROCEDURE RECEIVED PRODUCTS

PROCEDURE FOR THE PRODUCTION OF A HOLLOW FIBER DIAPHRAGM

Procedure for the enrichment of Polypeptiden, preferably of enzymes and enzyme inhibitors

LIPASE ON SOLID PHASE

Immobilized enzyme complexes and related methods

Immobilized enzyme complexes (IEC) with enzymes that are non-covalently linked to matrices are provided along with methods for making the same. Methods of using the IEC for a wide variety of industrial enzymatic processes are also provided. Methods of converting cellulosic biomass and methods of effecting blood type conversions with the IEC are amongst the methods disclosed.

PROCESS FOR PREPARING PHENYLALANINE

Fermentation process

Microporous pathogen-killing composition

METHOD OF IMMOBILIZING A BIOLOGIC IN A POLYURETHANE-HYDROGEL COMPOSITION, A COMPOSITION PREPARED FROM THE METHOD, AND BIOMEDICAL APPLICATIONS

WATER-INSOLUBLE ENZYMES

POLYAMIDE CARRIER FOR FIXING BIOLOGICALLY ACTIVE PROTEINS

Biointerface layer for analyte sensors

Disclosed are devices for determining an analyte concentration (e.g., glucose). The devices comprise a sensor configured to generate a signal associated with a concentration of an analyte and a sensing membrane located over the sensor. The sensing membrane comprises a biointerface layer which interfaces with a biological fluid containing the analyte to be measured. The biointerface layer comprises a biointerface polymer, wherein the biointerface polymer comprises polyurethane and/or polyurea segments and one or more zwitterionic repeating units. The biointerface layer increases sensor longevity and decrease sensor inaccuracy by inhibiting accumulation of cells, proteins, and other biological species on the outermost layers of the sensor.

Enzyme immobilized adhesive layer for analyte sensors

Disclosed are devices for determining an analyte concentration (e.g., glucose). The devices comprise a sensor configured to generate a signal associated with a concentration of an analyte and a sensing membrane located over the sensor. The sensing membrane comprises an enzyme layer, wherein the enzyme layer comprises an enzyme and a polymer comprising polyurethane and/or polyurea segments and one or more zwitterionic repeating units. The enzyme layer protects the enzyme and prevents it from leaching from the sensing membrane into a host or deactivating.

LIPASE AND OTHER PROTEINS PHYSICALLY BOUND TO A SOLID SUPPORT

Reversibly soluble enzyme-polymer conjugates

LONG ACTIVE FOOD GRADE OXYGEN SCAVENGER THAT CAN WITHSTAND PASTEURIZATION

METHOD FOR PREPARING A SUBSTRATE FOR IMMOBILISING A CELL, SAID SUBSTRATE AND USES THEREOF

La présente invention concerne un procédé de préparation d'un support sol ide susceptible d'immobiliser au moins une cellule et/ou au moins une partie de cellule, ledit procédé comprenant une étape consistant à fixer audit sup port solide un composé fusogène capable de s'insérer dans les membranes cell ulaires. La présente invention concerne également un procédé pour immobilise r au moins une cellule et/ou au moins une partie de cellule utilisant le sup port solide ainsi préparé, ledit support solide et ses utilisations dans le domaine du diagnostic biomédical ou de la surveillance sanitaire de fluides biologiques ou destinés à l'usage humain ou animal.

PROCESS FOR PREPARING AN IMMOBILIZED ENZYME

COVALENTLY IMMOBILIZED ENZYME AND METHOD TO MAKE THE SAME

BIOINTERFACE LAYER FOR ANALYTE SENSORS

Disclosed are devices for determining an analyte concentration (e.g., glucose). The devices comprise a sensor configured to generate a signal associated with a concentration of an analyte and a sensing membrane located over the sensor. The sensing membrane comprises a biointerface layer which interfaces with a biological fluid containing the analyte to be measured. The biointerface layer comprises a biointerface polymer, wherein the biointerface polymer comprises polyurethane and/or polyurea segments and one or more zwitterionic repeating units. The biointerface layer increases sensor longevity and decrease sensor inaccuracy by inhibiting accumulation of cells, proteins, and other biological species on the outermost layers of the sensor.

METHODS AND USES OF ENCAPSULATED EXUDATES AND DRIED EUGLENA BIOMASS FOR BINDING METAL

A method of binding a target metal in solution. The method of binding a target metal comprises contacting a solution containing i) a target metal with ii) an encapsulated exudate of a culture of algal flagellate, or a fraction thereof; or an encapsulated dried Euglena biomass or a fraction thereof, to form a complex between the target metal, and the encapsulated exudate or fraction thereof, or the encapsulated dried Euglena biomass or the fraction thereof; and optionally separating the complex from the solution. The disclosure also relates to a biosorbent element, as well as methods of using same in binding a metal in solution.

ENZYME PREPARATIONS

The invention is directed to enzyme preparations which are obtainable by enzyme immobilizates which comprise enzymes or microorganisms containing enzymes immobilized on an inert carrier being provided with a silicone coating obtained by hydrosilylation, to a process for producing such enzyme preparations and to the use of enzyme preparations as an industrial biocatalyst.

HIGH LOAD ENZYME IMMOBILISATION BY CROSSLINKING

The present invention relates to a method for cross-linking polypeptide molecules, comprising the step of combining a cross-linking agent and polypeptide molecules in solution under conditions suitable for a cross-linking reaction to occur and to a preparation comprising cross-linked polypeptide molecules obtainable by said method. The present invention further relates to cross-linked polypeptide molecules, wherein said polypeptide molecules are cross-linked by essentially unbranched cross-linking groups comprising at least 40 contiguous atoms. Moreover, the present invention relates to a test chemistry matrix comprising a redox cofactor, an agent capable of eliciting a change in at least one measurable property of an indicator reagent in the presence of redox equivalents, and an indicator reagent, wherein said chemistry matrix further comprises a preparation of cross-linked polypeptide molecules obtainable by the method according to the present invention and/or cross-linked polypeptide ...

CELL CULTURING METHOD, CELL CULTURING APPARATUS AND KIT

The present invention relates to a cell culturing method as well as a cell culturing apparatus and kit for use in said culturing method. This cell culturing method comprises applying cells on a porous polyimide film and culturing. One embodiment of the method according to the present invention comprises a process for sowing cells on the surface of the porous polyimide film. An embodiment comprises a process for placing a cell suspension on the dried surface of the porous polyimide film and leaving the porous polyimide film undisturbed or moving said porous polyimide film to promote liquid efflux or stimulating a portion of the surface to cause the cell suspension to be drawn into the film and then retaining the cells in the cell suspension inside the film while allowing the moisture to flow out. Another embodiment comprises a process for moistening one or both surfaces of the porous polyimide film with a cell culture solution or sterilized liquid, loading a cell suspension on the moistened ...

INTERACTIVE SYSTEM FOR PRESENTING AND ELIMINATING SUBSTANCES

The present invention relates to an interactive system comprising at least one active surface of plastic from monomers containing at least one structural element derived from a carbon dioxide (A), and at least one substance associated to a linker with at least one structural element (B) capable of establishing a hydrogen bond, and involving an interaction between the structural elements (A) and (B). That interactive system is suitable for presenting and eliminating substances in liquids.

NON-ANTIGENIC BRANCHED POLYMER CONJUGATES

Branched, substantially non-antigenic polymers are disclosed. Conjugates prepared with the polymers and biologically active molecules such as proteins and peptides demonstrate extended circulating life in vivo. Substantially fewer sites on the biologically active material are used as attachment sites. Methods of forming the polymer, conjugating the polymers with biologically active moieties and methods of using the conjugates are also disclosed.

Procédé pour fixer des composés organiques à des polymères par des liaisons covalentes

Verfahren zur Anreicherung von Proteinen

PROCEDE DE FABRICATION D'UNE PREPARATION ENZYMATIQUE INSOLUBLE DANS L'EAU.

Verfahren zur Herstellung von oberflächlich durch aufgepfropftes Polymerisat modifiziertem Polymermaterial

Procédé de séparation d'un polypeptide à partir d'un liquide aqueux en vue de sa détermination qualitative ou quantitative

[...][...][...][...] DE [...][...][...] LA [...] DE.

PROCEDURE FOR THE EXECUTION OF AN ENZYMATIC REACTION.

PROCEDURE FOR THE PRODUCTION OF CARRIER-BOUND PROTEINS.

Process for the preparation of biologically active compounds, and the use thereof

Method for suppressing the immunogenic action of a biocatalyst.

MICROORGANISMS OF ABSTENTIONS, HYDROPHILIC FOAM, PROCEDURE FOR ITS PRODUCTION AS WELL AS USE OF THE SAME FOR THE PRODUCTION OF L-AMINO ACIDS.

Process for preparing a biological substance which is bound to a poly(hydroxymethylene) support matrix

PROCEDURE FOR THE ADJUSTMENT OF BIOLOGICALLY ACTIVE PROTEIN OR OF SUBSTRATE OF BIOLOGICALLY ACTIVE PROTEIN AT PP.

Phenylene group-containing organopolysiloxanes modified with functional groups and method of their preparation

Functionalized phenylene group-containing organopolysiloxanes, comprising a plurality of identical or different units of the formula: (1) where in each case all three possible isomers in relation to the position of the SiO3/2-R1 substituents on the phenylene group can be present concurrently, in which the bridge groups R1 represent the groups -CH2-CH2- or CH3-CH<, X represents Cl, Br, CH2Cl, P(C6H5)2, and Ch2P(C6H5)2, and the free valences of the oxygen atoms are saturated by silicon atoms of other groups of formula (1) and/or by cross-linking agents. Also disclosed is a process for the preparation of said polysiloxanes.

Detergent compositions comprising immobilized enzymes

PCT No. PCT/US95/17039 Sec. 371 Date Jun. 29, 1998 Sec. 102(e) Date Jun. 29, 1998 PCT Filed Dec. 29, 1995 PCT Pub. No. WO97/24421 PCT Pub. Date Jul. 10, 1997The present invention relates to detergent compositions comprising one or more enzymes which are immobilized by a covalent binding on an activated polymer. Such enzymes are preferably bound to the activated polymer via a spacer molecule. The enzymes are selected from a variety of enzymes including, for example, cellulases, hemicellulases, peroxidases, proteases, glucoamylases, amylases, lipases, cutinases, pectinases, reductases, oxidases, phenoloxidases, lipoxygenases, laccases, ligninases, pullulanases, xylanases, tannases, pentosanases, manlanases, beta -glucanases, arabinosidases, and mixtures thereof. The polymer is selected from a variety of polymers. A preferred polymer for use in the present invention is polyethylene glycol.

Methods And Materials For Microorganism Capture

Material complexes that capture biologicals and methods of synthesizing and using such complexes composed of fluid-insoluble material and a receptor are provided herewith. The fluid-insoluble material has reactive functionality on its surface, including hydroxyl, amino, mercapto or eposy functionality material. The material can be agarose, sand, textile, or any combination thereof. The receptor is selected from the group consisting of mono-and poly-saccharides, heparin, or any combination thereof. Also provide are methods whereby releasing the captured biologicals and is controllable.

SITE-SPECIFIC CONJUGATION TO ANTIBODY LYSINE RESIDUES WITH SOLID-PHASE IMMOBILIZED MICROBIAL TRANSGLUTAMINASE MTG AND MTG IN SOLUTION

Site-specific modification of proteins with microbial transglutaminase (MTG) is a powerful and versatile strategy for a controlled modification of proteins under physiological conditions. Solid-phase microbead-immobilization is used to site-specifically and efficiently attach different functional molecules important for further downstream applications to proteins of therapeutic relevance including scFV, Fab-fragment and antibodies. MTG remained firmly immobilized with no detectable column bleeding and enzyme activity was sustained during continuous operation. Immobilized MTG shows enhanced selectivity towards a certain residue in the presence of several reactive residues which are all targeted when the conjugation was carried out in solution. The generation of dual site-specifically conjugated IgG1 with immobilized and MTG in solution is reported, i.e. site-specific conjugation to glutamine and lysine residues of IgG1 antibody. Site-specific glutamine conjugation with small peptides containing ...

Complex, Preparation Methods and Application Thereof

The invention is directed to a complex, including: a porous composite carrier including: a porous organic foam material containing open pores, each pore comprising a wall defining the pore; and a crosslinked product having aldehyde groups and immobilized on the surface of the walls of one or more pores of the porous organic foam materials, and a protein, polypeptide, or oligopeptide immobilized onto the porous composite carrier through a reaction between an amino group of the protein, polypeptide or oligopeptide and an aldehyde group of the composite carrier. The immobilized product has high specific surface area and high specific activity. The immobilization is simple and in low cost, and is suitable for industrial application.

SYSTEMS AND METHODS FOR IDENTIFYING RARE CELLS

Disclosed herein are compositions and methods of fixing and staining rare cells. Further, disclosed herein are methods of identifying circulating tumor cells (CTC). In some embodiments, the method includes: imaging a cell sample to identify a cell of interest; determining a first pixel intensity of a stained nuclear area; determining a second pixel intensity of a background area; calculating a ploidy status of the cell of interest by subtracting the second pixel intensity from the first pixel intensity; and determining whether the cell of interest is a CTC based on the ploidy status. The method may be computer implemented, such that the method uses a machine learning algorithm to identify a feature; process the feature to extract a parameter of interest; analyze the parameter of interest; and when the parameter of interest is greater than or less than a pre-determined threshold, classify the cell of interest as a CTC.

ИМПЛАНТАЦИЯ ИНКАПСУЛИРОВАННЫХ БИОЛОГИЧЕСКИХ МАТЕРИАЛОВ ДЛЯ ЛЕЧЕНИЯ ЗАБОЛЕВАНИЙ

Изобретение относится к области лекарственных средств, в частности к составам и способам лечения заболевания, в частности диабета, путем имплантации инкапсулирующих устройств, содержащих покрытие и клетки, при этом плотность клеток составляет по меньшей мере 100000 клеток/мл, а покрытие содержит акрилатный полиэтиленгликоль (PEG) высокой плотности с молекулярной массой от 900 до 3000 дальтон, а также сульфонированный сомономер. Кроме того, изобретение относится к способу получения указанного состава. Технический результат заключается в минимизации тканевого ответа, увеличению концентрации клеток и увеличению времени жизнеспособности клеток в указанных устройствах. 5 н. и 78 з.п. ф-лы, 34 ил., 8 табл.

СПОСОБ ПОЛУЧЕНИЯ 2-ГИДРОКСИ-4-МЕТИЛТИОМАСЛЯНОЙ КИСЛОТЫ И/ИЛИ ЕЕ АММОНИЕВОЙ СОЛИ И ВОДНЫЙ РАСТВОР УКАЗАННОЙ КИСЛОТЫ И ЕЕ СОЛИ

Изобретение относится к биотехнологии. На первой стадии получают биологический материал, обладающий нитрилазной активностью. На второй стадии этот материал фиксируют. На третьей стадии 2-гидрокси-4-метилтиобутиронитрил вводят во взаимодействие с фиксированным биологическим материалом, получая аммониевую соль 2-гидрокси-4-метилтиомасляной кислоты. На четвертой стадии, в случае необходимости, соль, полученную на третьей стадии, превращают в соответствующую кислоту. На пятой стадии концентрируют продукт, полученный на третьей и четвертой стадиях. Водный раствор содержит смесь указанной соли и кислоты, в которой массовая доля кислоты составляет 0,05-0,99. Способ применим в промышленном масштабе с повышенным выходом без использования растворителей и без сопутствующего получения минеральных солей. 2 с. и 18 з.п.ф-лы, 12 ил., 4 табл.

КОНЬЮГАТЫ PEG-УРАТОКСИДАЗЫ ИХ ИСПОЛЬЗОВАНИЕ, СПОСОБ ИЗОЛИРОВАНИЯ ТЕТРАМЕРНОЙ ФОРМЫ УРИКАЗЫ

... 1. Конъюгат, содержащий уриказу, конъюгированную в PEG, причем PEG имеет средний молекулярный вес приблизительно от 5 кДа до 100 кДа, и при этом уриказа сохраняет по меньшей мере приблизительно 75% уриколитической активности неконъюгированной уриказы, и иммуногенность уриказы существенно снижена.2. Конъюгат по п.1, в котором упомянутый PEG является полиэти-ленгликолем.3. Конъюгат по п.2, в котором упомянутый полиэтиленгликоль имеет средний молекулярный вес примерно 20 kDa.4. Конъюгат по п.2, в котором упомянутый полиэтиленгликоль имеет средний молекулярный вес примерно 10 kDa.5. Конъюгат по п.2, в котором упомянутый полиэтиленгликоль имеет средний молекулярный вес примерно 30 kDa.6. Конъюгат по п.1, в котором упомянутая уриказа является тетрамерной.7. Фармацевтический состав для снижения уровней мочевой кислоты в жидкости или ткани тела, содержащий конъюгат по п.1 и фармацевтически приемлемый носитель.8. Конъюгат по п.1, в котором каждая под единица уриказы ковалентно связана со в среднем ...

CПOCOБ ПOЛУЧEHИЯ ИMMOБИЛИЗOBAHHЫX ДPOЖЖEЙ

Enzympräparate

LANGZEITLICH AKTIVES PASTEURISIERUNGSBESTAENDIGES DESOXIDIERUNGSMITTEL VON LEBENSMITTELQUALITAET

Organopolysiloxangel und ein darauf immobilisiertes Enzym.

ENZYMES

... 1463513 Enzymatic preparations BEECHAM GROUP Ltd 6 Aug 1975 [13 Aug 1974 26 April 1975] 35556/74 and 17434/75 Heading C3H [Also in Division C2] An enzyme preparation comprises an enzyme attached to sufficient non-polar groups, such that on contacting the preparation in aqueous media with an inert water-immiscible liquid, the preparation becomes associated with the inert water-immiscible liquid and is separable thereby from the aqueous medium. The enzyme is preferably attached to a polymeric material, e.g. cellulose powder, carboxymethylcellulose, ion exchange resins, nylon, agarose and cross-linked dextrans, polyacrylates and methacrylates and sucrose modified with epichlorohydrin. Specified enzymes are amylase, asparaginase, protease, chymotrypsin, cellulose, dextranase, lipase, oxynitrilase, pepsin, penicillin acylase and trypsin. Suitable non-polar groups which may be attached to the enzyme are hydrocarbyl groups having C 6 to C 30 of which many examples are provided. Examples describe ...

New Graft Copolymers

... 1,172,768. Determining human growth hormone. IMPERIAL CHEMICAL INDUSTRIES OF AUSTRALIA & NEW ZEALAND Ltd. 21 April, 1967 [26 April, 1966; 30 Dec., 1966], No. 18487/67. Heading B1X. [Also in Division C3] Human growth hormone is determined using a standard curve obtained by incubating fixed quantities of rabbit anti-human antibody supported on poly (tetrafluoroethylene-g-isothiocyanato-styrene) and I131-labelled human growth hormone with increasing quantities of unlabelled human growth hormone. The polymer is prepared by a method described in Group C3.

VERFAHREN ZUR FIXIERUNG VON BIOLOGISCH AKTIVEM PROTEIN AN POLYAMIDE

VERFAHREN ZUR HERSTELLUNG EINES NEUEN, BIOLOGISCH AKTIVEN, WASSERUNLOSLICHEN PRODUKTES

SUBSTRATE FOR CHROMATOGRAPHI USE OR TO WOULD DRIVE OUT FROM ENZYMATIC REACTIONS.

IMMOBILIZED CELLS.

POLYMERE SORBENTIEN ON BASIS OF POLYMERIZATION-ABLE DERIVATIVES OF PP

Procedure for the production of 6-Aminopenicillansäure by enzymatic splitting of Penicillinen

PROCEDURE FOR THE PRODUCTION OF A TRAGERMATERIALS FOR PROTEINS

Procedure for the production of carrier-bound proteins

Procedure for the production of kovalent at carriers of bound Penicillinacylase

IMMOBILIZATION OF COMPOUNDS ON POLYMERIC MATRIX

IMMOBILIZED CELLS AND LIPOSOMES AND METHOD OF IMMOBILIZING THE SAME

Sorption reinforced catalytic coating system and method for the degradation of threat agents

Sorption reinforced catalytic coating system for the degradation of threat agents including a synzyme coating about a material, the synzyme coating having bucket-shaped molecules for the sorption and degradation of the threat agents. A binding agent is configured for synzyme immobilization to maximize loading and retention of the synzyme coating on the material.

Thermostable sucrose phosphorylase

The present invention relates to a sucrose phosphorylase from Bifidobacterium adolescentis which is useful as a biocatalyst in carbohydrate conversions at high temperatures. Indeed, the biocatalysts of the present invention are enzymatically active for a time period of at least 16 h and up to 1 to 2 week(s) at a temperature of at least 60° C. The biocatalysts of the present invention are: a) immobilized on an enzyme carrier, or b) are part of a cross-linked enzyme aggregate (CLEA), and/or c) are mutated, and/or d) are enzymatically active in the continuous presence of their substrate.

Coatings Containing Polymer Modified Enzyme For Stable Self-Cleaning Of Organic Stains

Bioactive coatings that are stabilized against inactivation by weathering are provided including a base associated with a chemically modified enzyme, and, optionally a first polyoxyethylene present in the base and independent of the enzyme. The coatings are optionally overlayered onto a substrate to form an active coating facilitating the removal of organic stains or organic material from food, insects, or the environment.

Enzyme-fiber matrix composite of three-dimensional network structure, preparation method thereof, and use thereof

Disclosed is a composite of enzyme and fiber matrix with three-dimensional structure. The composite of enzyme and fiber matrix with three-dimensional structure includes a significantly large amount of an enzyme loaded in and immobilized in/onto a matrix when compared to conventional composites. In addition, the immobilized enzyme is prevented from leaching from the matrix when an external impact is applied to the composite of enzyme and fiber matrix with three-dimensional structure. Therefore, the stability of the composite of enzyme and fiber matrix with three-dimensional structure of the present invention is maintained even after a long period passes since a remarkably great amount of enzymes compared with a known composite can be supported and immobilized to a matrix, and the immobilized enzyme is not easily released by an external impact. In addition, it is possible to stably immobilize a great amount of enzymes even if a functional group covalently bonding to enzymes is hardly present on the surface of fiber. Therefore, it is possible to remarkably improve performance by using the composite of enzyme and fiber matrix with three-dimensional structure of the present invention in a biosensor, a bio-fuel cell and the like, compared with the case using a known matrix composite.

Micellar polysiloxane enzyme immobilization materials

The present invention generally relates to immobilized enzymes for use in carbon capture and other systems; particularly, materials used to immobilize carbonic anhydrase are disclosed.

CONTINUOUS FLOW METHOD FOR PREPARING (R)-3-HYDROXY-5-HEXENOATE

Disclosed herein relates to biopharmaceuticals, and more particularly to a continuous flow method for preparing (R)-3-hydroxy-5-hexenoate. Carbonyl reductase and isopropanol dehydrogenase are co-immobilized onto an inert solid medium simultaneously to prepare a carbonyl reductase/isopropanol dehydrogenase co-immobilized catalyst, which is then filled into a microchannel reactor of the micro reaction system. A solution containing substrate 3-carbonyl-5-hexenoate is subsequently pumped into the microchannel reactor to perform an asymmetric carbonyl reduction reaction to obtain (R)-3-hydroxy-5-hexenoate. 2. The method of claim 1 , wherein in step (1) claim 1 , the inert solid medium is a composite material of polyvinyl alcohol and polyethylene glycol; and the step of “co-immobilizing a carbonyl reductase and an isopropanol dehydrogenase onto an inert solid medium simultaneously to prepare the co-immobilized catalyst” comprises:(a) preparing an aqueous solution of the polyvinyl alcohol and the polyethylene glycol; heating the aqueous solution until the aqueous solution becomes clear; and cooling the aqueous solution to 50° C. or less to obtain a first solution;(b) adding a crude carbonyl reductase solution and a crude isopropanol dehydrogenase solution into the first solution followed by uniform mixing to obtain a second solution; and(c) dropwise adding the second solution onto a polyethylene film; drying the polyethylene film at 35-40° C. for 0.5-1 hour to obtain the co-immobilized catalyst; and storing the co-immobilized catalyst at 4° C. for later use;wherein an amino acid sequence of the carbonyl reductase is shown in SEQ ID NO: 1; and an amino acid sequence of the isopropanol dehydrogenase is shown in SEQ ID NO: 2;a weight ratio of the polyvinyl alcohol to the polyethylene glycol is 5:1-3;the crude carbonyl reductase solution and the crude isopropanol dehydrogenase solution both have an initial concentration of 10%-30% (w/v); andin step (b), a volume ratio of the ...

DEVICE

The present invention provides a nerve cell device in which early observation of nerve activity (spikes, bursts, and the like) is made possible and the measured electric strength is increased by cultivating neurons upon a cell scaffold. By using this nerve cell device, imaging of intracellular signaling is also possible. 1. A nerve cell device comprising a cell scaffold and neurons.2. The nerve cell device according to claim 1 , wherein the neurons are oriented.3. The nerve cell device according to claim 1 , wherein the cell scaffold is a fiber sheet formed of a polymeric material.4. The nerve cell device according to claim 3 , wherein the fiber sheet has an oriented structure claim 3 , a non-oriented structure or a mixed structure of orientation and non-orientation.5. The nerve cell device according to claim 3 , wherein the fiber sheet is coated with the extracellular matrix protein selected from polylysine claim 3 , polyornithine claim 3 , laminin claim 3 , fibronectin claim 3 , MATRIGEL® and GELTREX®.6. The nerve cell device according to claim 1 , wherein the neurons form a three-dimensional structure on the cell scaffold and/or in the cell scaffold.7. The nerve cell device according to claim 1 , wherein the neurons are neural cells derived from primary cultured cells or pluripotent stem cells.8. The nerve cell device according to claim 7 , wherein the neural cells derived from the primary cultured cells or pluripotent stem cells are neural cells derived from mammals.9. The nerve cell device according to claim 1 , wherein the neurons comprise glutamatergic claim 1 , dopaminergic claim 1 , gamma-aminobutyratergic claim 1 , monoaminergic claim 1 , histaminergic or cholinergic neurons.10. The nerve cell device according to claim 1 , wherein: the neurons are seeded at a density of 1×10cells/cmto 4×10cells/cmagainst the cell scaffold.11. The nerve cell device according to claim 1 , further comprising: a frame for holding the periphery of the nerve cell device.12. The ...

Enzyme forming mesoporous assemblies embedded in macroporous scaffolds

A hierarchical catalyst composition comprising a continuous or particulate macroporous scaffold in which is incorporated mesoporous aggregates of magnetic nanoparticles, wherein an enzyme is embedded in mesopores of the mesoporous aggregates of magnetic nanoparticles. Methods for synthesizing the hierarchical catalyst composition are also described. Also described are processes that use the recoverable hierarchical catalyst composition for depolymerizing lignin, remediation of water contaminated with aromatic substances, polymerizing monomers by a free-radical mechanism, epoxidation of alkenes, halogenation of phenols, inhibiting growth and function of microorganisms in a solution, and carbon dioxide conversion to methanol. Further described are methods for increasing the space time yield and/or total turnover number of a liquid-phase chemical reaction that includes magnetic particles to facilitate the chemical reaction, the method comprising subjecting the chemical reaction to a plurality of magnetic fields of selected magnetic strength, relative position in the chemical reaction, and relative motion.

HYDROGEL-ENCAPSULATED CELLS AND HYDROGEL-DISPERSED CELLS

Embodiments of the present disclosure generally relate to compositions that include hydrogel-encapsulated/dispersed cells, compositions including hydrogel-encapsulated/dispersed cells, and to processes for forming such hydrogel-encapsulated/dispersed cells and compositions thereof. The compositions can be used for, e.g., therapeutic applications. In some examples, the hydrogel-encapsulated/dispersed cells are formed using photoreactive groups chemically attached to polyethylene glycol to form a material which, upon exposure to a desired wavelength or wavelength range of light, reacts to form a cross-linked hydrogel network. 1. A composition , comprising:a microcapsule comprising a core and a polymeric shell enclosing the core, the core comprising a cell, and the polymeric shell comprising, in polymerized form, one or more photoreactive monomers and a linker.2. The composition of claim 1 , wherein the one or more photoreactive monomers comprise a methylene functional group claim 1 , an acid functional group claim 1 , or combinations thereof.3. The composition of claim 2 , wherein claim 2 , when the one or more photoreactive monomers comprise a methylene functional group claim 2 , the one or more photoreactive monomers comprise polyethylene glycol norbornene claim 2 , polyethylene glycol diacrylate claim 2 , derivatives thereof claim 2 , or combinations thereof.4. The composition of claim 2 , wherein claim 2 , when the one or more photoreactive monomers comprise an acid functional group claim 2 , the one or more photoreactive monomers comprise polylactic acid claim 2 , derivatives thereof claim 2 , or combinations thereof.5. The composition of claim 1 , wherein the linker is a dithiol linker.6. The composition of claim 5 , wherein the dithiol linker is a polyethylene glycol-dithiol.7. The composition of claim 1 , wherein:the linker has a molecular weight from about 500 Da to about 10,000 Da;the one or more photoreactive monomers has a molecular weight from about 250 ...

GAS BIOCATALYSIS VIA AN IMMOBILIZED CELL BIOREACTOR

A method for forming a product from a gas includes flowing a gas through a bioreactor for contacting the gas with polymer-immobilized whole cells and collecting the product from the bioreactor. The bioreactor includes a plurality of printed three-dimensional structures, each printed three-dimensional structure having at least one sidewall being a lattice, and the polymer-immobilized whole cells being present in the lattice.

Aqueous Systems Of At Least Two Phases Containing Microcapsules And Processes For Manufacturing The Same

In one aspect of the invention, a microcapsule includes a film encapsulating a material. The film is formed by complexation of at least two mutually attractive components initially present in an aqueous dispersion comprising a continuous phase and a dispersed phase. The at least one first component is initially present in the continuous phase and the at least one second component is initially present in the dispersed phase. According to another aspect of the invention, provided is a process for forming microcapsules including the step of injecting a dispersed phase having at least a first component into a continuous phase having at least a second component, where the first component and the second component are mutually attractive, such that a film is formed by complexation of the first charged component and the second charged component. 1. An aqueous dispersion , comprising:a continuous phase;a dispersed phase; and the microcapsule encapsulating an amount of the dispersed phase and optionally encapsulating an amount of the continuous phase, and', 'the microcapsule comprising a complexation of first and second mutually attractive components., 'a microcapsule dispersed within the continuous phase;'}2. The aqueous dispersion of claim 1 , wherein the first mutually attractive component is initially present in the continuous phase and the second mutually attractive component is initially present in the dispersed phase.3. The aqueous dispersion of claim 1 , wherein the first and second mutually attractive components are attracted by a force selected from electric charges claim 1 , intermolecular forces claim 1 , hydrogen bonding claim 1 , metal coordination claim 1 , and host-guest interactions.4. The aqueous dispersion of claim 1 , wherein (a) at least one of the continuous phase and dispersed phase comprises at least 5 wt % polyethylene glycol (PEG) claim 1 , (b) at least one of the continuous phase and the dispersed phase has a composition that comprises at least 5 wt ...

Hierarchical magnetic nanoparticle-enzyme mesoporous assemblies embedded in macroporous scaffolds

A hierarchical catalyst composition comprising a continuous or particulate macroporous scaffold in which is incorporated mesoporous aggregates of magnetic nanoparticles, wherein an enzyme is embedded in mesopores of the mesoporous aggregates of magnetic nanoparticles. Methods for synthesizing the hierarchical catalyst composition are also described. Also described are processes that use the recoverable hierarchical catalyst composition for depolymerizing lignin, remediation of water contaminated with aromatic substances, polymerizing monomers by a free-radical mechanism, epoxidation of alkenes, halogenation of phenols, inhibiting growth and function of microorganisms in a solution, and carbon dioxide conversion to methanol. Further described are methods for increasing the space time yield and/or total turnover number of a liquid-phase chemical reaction that includes magnetic particles to facilitate the chemical reaction, the method comprising subjecting the chemical reaction to a plurality of magnetic fields of selected magnetic strength, relative position in the chemical reaction, and relative motion.

Coatings containing polymer modified enzyme for stable self-cleaning of organic stains

Bioactive coatings that are stabilized against inactivation by weathering are provided including a base associated with a chemically modified enzyme, and, optionally a first polyoxyethylene present in the base and independent of the enzyme. The coatings are optionally overlayered onto a substrate to form an active coating facilitating the removal of organic stains or organic material from food, insects, or the environment.

Compositions and methods for urinary bladder regeneration

The present invention provides compositions and methods for the regeneration of tissue. In particular, the present invention provides mesenchymal stem cells (MSCs) and endothelial progenitor cells (EPCs) for bladder regeneration.

Process for making cell populations of the hepatic lineage from endodermal cells and cellular compositions comprising same

The present disclosure concerns processes as well as additives for differentiating an endodermal cells into a posterior foregut cell, a posterior foregut cell into an hepatic progenitor cell and/or an hepatic progenitor cell into an hepatocyte-like cell. In some embodiments, the process can be conducted in the absence of serum. The hepatocyte-like cell population obtained from this process have a detectable Cyp3A4 activity and/or express a detectable level of albumin and/or of urea. The process can be designed to increase cellular yield.

Method for producing a protein-functionalized film as well as protein functionalized film

The present invention relates to a method for producing a protein-functionalized film, in which a protein is bound to a copolymer or a polymer having an unhydrolyzed or hydrolyzed thiolactone functionalization is bound to the film by means of the existing functionalization. The present invention also relates to a correspondingly produced film.

Fuel biocell

The invention relates to nanoparticles, preferably metal oxides, at least partially coated on the surface by a silylated polymer, and functionalised by at least one molecule of an oxidation-reduction mediating agent, characterised in that the immobilisation of said molecule(s) of said mediating agent on the surface of said nanoparticles is enabled by non-covalent bonds, preferable π-π type interactions, established between ethylenic, acetylenic and/or aromatic patterns, respectively present in the region of said silylated polymer and said molecule(s) of the oxidation-reduction mediating agent.

Diffusion resistance layer for analyte sensors

Disclosed are devices for determining an analyte concentration (e.g., glucose). The devices comprise a sensor configured to generate a signal associated with a concentration of an analyte and a sensing membrane located over the sensor. The sensing membrane comprises an enzyme layer, wherein the enzyme layer comprises an enzyme and a polymer comprising polyurethane and/or polyurea segments and one or more zwitterionic repeating units. The enzyme layer protects the enzyme and prevents it from leaching from the sensing membrane into a host or deactivating.

Nanozymes, methods of making nanozymes, and methods of using nanozymes

Embodiments of the present disclosure provide for nanozymes that can include a therapeutic agent, methods of making nanozymes, methods of using nanozymes, and the like.

POLYMERIC ENCAPSULATION OF WHOLE CELLS AS BIOREACTORS

In one inventive concept, a mixture for forming polymer-encapsulated whole cells includes a pre-polymer, a photoinitiator, and a plurality of whole cells. In another inventive concept, a product includes a structure including a plurality of whole cells encapsulated in a polymer, where the polymer is cross-linked. 1. A mixture for forming polymer-encapsulated whole cells , the mixture comprising:a pre-polymer;a photoinitiator; anda plurality of whole cells.2. The mixture as recited in claim 1 , wherein the pre-polymer includes at least one pre-polymer selected from the group consisting of: poly(ethylene) glycol claim 1 , amphiphilic silicones claim 1 , alginate claim 1 , N-isopropylacrylamide claim 1 , and methacrylic acid.3. The mixture as recited in claim 2 , wherein the pre-polymer is poly(ethylene) glycol acrylate.4. The mixture as recited in claim 2 , wherein a concentration of the pre-polymer is in a range of about 10 weight % to about 50 weight % of a total weight of the mixture.5. The mixture as recited in claim 1 , wherein a molecular weight of the pre-polymer is in a range of about 575 Daltons to about 100 claim 1 ,000 Daltons.6. The mixture as recited in claim 1 , wherein a molecular weight of the pre-polymer is in a range of about 10 claim 1 ,000 Daltons to about 40 claim 1 ,000 Daltons.7. The mixture as recited in claim 1 , wherein the whole cells are whole living cells.8. The mixture as recited in claim 1 , wherein the whole cells are dried whole cells.9. The mixture as recited in claim 1 , wherein the whole cells have a characteristic to convert a chemical reactant to a product claim 1 , wherein the chemical reactant is a gas and the product is a liquid.10. The mixture as recited in claim 1 , wherein the whole cells are configured to convert methane to methanol.11. The mixture as recited in claim 1 , wherein the whole cells are selected from the group consisting of: methanotrophic organisms claim 1 , methylotrophic organisms claim 1 , and yeast.12. The ...

Aqueous systems of at least two phases containing microcapsules and processes for manufacturing the same

In one aspects of the invention, a microcapsule includes a film encapsulating a material. The film is formed by complexation of at least two mutually attractive components initially present in an aqueous dispersion comprising a continuous phase and a dispersed phase. The at least one first component is initially present in the continuous phase and the at least one second component is initially present in the dispersed phase. According to another aspect of the invention, provided is a process for forming microcapsules including the step of injecting a dispersed phase having at least a first component into a continuous phase having at least a second component, where the first component and the second component are mutually attractive, such that a film is formed by complexation of the first charged component and the second charged component.

Immobilized enzymes

Immobilized enzymes may be produced by reacting enzymes with activated substrates which contain as activated groups oxiran or azlactone groups as substituents in linear polymers. These immobilized enzymes are used in reaction with high molecular substrates. The immobilized proteinase K, for example, is suitable for inactivating other enzymes, in particular nucleic acid-modifying enzymes, for example restriction endonucleases.

Functional porous fibres

The invention relates to a method for the preparation of porous polymeric fibres comprising functionalized or active particles. By extruding a mixture of one or more dissolved polymers with particulate material a porous fibre is obtained in which the particulate material is entrapped. Extrusion of the fibre occurs under two-step phase inversion conditions. In particular the porous fibres can be used for the isolation of macromolecules such as peptides, proteins, nucleic acids or other organic compounds from complex reaction mixtures, in particular from fermentation broths. Another application is the immobilization of a catalyst in a reaction mixture.

Polyglutaraldehyde synthesis and protein bonding substrates

Polyglutaraldehyde (PGL) is polymerized in aqueous base or in aqueous highly polar solvent basic media to prepare powders, castable films or coatings for substrates such as amine substituted microbeads. PGL microspheres can be prepared by suspension polymerization in presence of a surfactant or by precipitating PGL from solution containing surfactant. Magnetic PGL microspheres are formed by suspension polymerization in the presence of magnetic particles such as iron oxide. Polyglutaraldehyde can be converted to a fluorescent polymer by reaction with m-aminophenol or other reagent. Proteins can be readily covalently bound to the polyglutaraldehyde.

Systems and methods for identifying rare cells

Disclosed herein are compositions and methods of fixing and staining rare cells. Further, disclosed herein are methods of identifying circulating tumor cells (CTC). In some embodiments, the method includes: imaging a cell sample to identify a cell of interest; determining a first pixel intensity of a stained nuclear area; determining a second pixel intensity of a background area; calculating a ploidy status of the cell of interest by subtracting the second pixel intensity from the first pixel intensity; and determining whether the cell of interest is a CTC based on the ploidy status. The method may be computer implemented, such that the method uses a machine learning algorithm to identify a feature; process the feature to extract a parameter of interest; analyze the parameter of interest; and when the parameter of interest is greater than or less than a predetermined threshold, classify the cell of interest as a CTC.

Compositions and methods for identifying rare cells

Disclosed herein are compositions and methods of fixing and staining rare cells. Farther, disclosed herein are methods of identifying circulating tumor cells (CTC). In some embodiments, the method includes: imaging a cell sample to identity a cell of interest; determining a first pixel intensity of a stained nuclear area; determining a second pixel intensity of a background area; calculating a ploidy status of the cell of interest by subtracting the second pixel intensity from the first pixel intensify; and determining whether the cell of interest is a CTC based on the ploidy status. The method may be computer implemented, such, that the method uses a machine learning algorithm to identify a feature; process the feature to extract a parameter of interest; analyze the parameter of interest; and when the parameter of interest is greater than or less than a predetermined threshold, classify the cell of interest as a CTC.

Novel biocatalyst compositions and processes for use

The microorganism-containing biocatalysts disclosed have a large population of the microorganisms irreversibly retained in the interior of the biocatalysts. The biocatalysts possess a surprisingly stable population of microorganisms and have an essential absence of debris generation from metabolic activity of the microorganisms. The biocatalysts are composed of highly hydrophilic polymer and have an internal, open, porous structure that promotes community phenotypic changes.

Fremgangsmaade til fremstilling af et partikelformet, immobiliseret produkt og anvendelse af immobiliseret enzym fremstillet herved

A PROCEDURE FOR IMMOBILIZING AN AQUEOUS DISPERSION OF ENZYMATICALLY ACTIVE MICROORGANISM CELLS AND CELLULAR SUBSTANCES

加速捕捉二氧化碳的方法

本发明通常涉及气流，尤其是烟道气体、来自转化炉的氢气、天然气、或来自水泥窑的气体中二氧化碳的去除。还公开了用于碳捕捉的经固定的酶和其他系统。

효소의 고정화방법, 구체적으로는 세포결합효소를 세포군입자로 전환시키는 방법

내용 없음

Non-antigenic branched polymer conjugates

Branched, substantially non-antigenic polymers are disclosed. Conjugates prepared with the polymers and biologically active molecules such as proteins and peptides demonstrate extended circulating life in vivo. Substantially fewer sites on the biologically active material are used as attachment sites. Methods of forming the polymer, conjugating the polymers with biologically active moieties and methods of using the conjugates are also disclosed.

Immobilization of chemical species in crosslinked matrices

A method of immobilizing a chemical specie in a three-dimensional, crosslinked matrix. The method involves bringing together in covalent bonding proximity a desired chemical specie and a polymeric coupling compound having at least two latent reactive groups per molecule, each latent reactive group being capable when activated of covalently bonding to another coupling compound molecule or to the chemical specie. The chemical specie desirably is free of latent reactive groups that are activated upon activation of the latent reactive groups are simultaneously activated to cause formation via covalent bonding of a three-dimensional molecular network in which molecules of the chemical specie are covalently bonded to molecules of the coupling compound, and molecules of the coupling compound are covalently bonded to each other. Preferably, the three-dimensional matrix is formed as a coating upon a surface to which covalent bonds are formed upon activation of the latent reactive groups of the coupling compound.

폴리아제티딘과의 교차결합에 의한 생물학적 물질의 고정방법

내용 없슴.

固定化羰基还原酶及其在制备(2r,3s)-2-羟基-4-苯基丁烷衍生物的应用

本发明公开了固定化羰基还原酶及其在制备(2R,3S)‑2‑羟基‑4‑苯基丁烷衍生物的应用，通过基因突变和增加特定肽链的方式，获得了具备在高浓度底物和有机溶剂的反应体系中实现不低于95％的转化率的固定化羰基还原酶；该酶活性受到共价结合固定化酶制法的影响非常小，可重复用于生产制备。

바이오애노드, 바이오캐소드 및 바이오연료 전지 내의효소를 사용하는 직접 전자 전달

본 발명은 전자 도체, 하나 이상의 애노드 효소 또는 캐소드 효소 및 효소 고정화 물질을 포함하는 바이오애노드, 바이오캐소드 및 바이오연료 전지에 관한 것이다. 애노드 효소는 연료 유체와 반응하여 산화된 형태의 연료 유체를 생성할 수 있으며, 전자 도체로 전자를 방출할 수 있다. 캐소드 효소는 산화제와 반응하여 물을 생성할 수 있으며, 전자 도체로부터 전자를 수득할 수 있다. 애노드 효소 및 캐소드 효소 둘 모두에 대한 효소 고정화 물질은 효소를 고정화 및 안정화시킬 수 있으며, 연료 유체 및/또는 산화제에 투과성이다. 바이오애노드, 바이오캐소드, 바이오연료 전지, 효소 고정화 물질

Non-antigenic branched polymer conjugates

Branched, substantially non-antigenic polymers are disclosed. Conjugates prepared with the polymers and biologically active molecules such as proteins and peptides demonstrate extended circulating life in vivo. Substantially fewer sites on the biologically active material are used as attachment sites. Methods of forming the polymer, conjugating the polymers with biologically active moieties and methods of using the conjugates are also disclosed.

Water-soluble non-antigenic polymer linkable to biologically active material

Branched, substantially non-antigenic polymers are disclosed. Conjugates prepared with the polymers and biologically active molecules such as proteins and peptides demonstrate extended circulating life in vivo. Substantially fewer sites on the biologically active material are used as attachment sites. Methods of forming the polymer, conjugating the polymers with biologically active moieties and methods of using the conjugates are also disclosed.

Method of immobilizing proteinaceous substances

Proteins such as enzymes are immobilized on a microporous member comprising a binder or matrix and finely divided filler particles dispersed throughout the binder. The proteins are coupled to the filler particles, and the microporous member has a relatively large surface area and a large number of available protein coupling sites. Enzymes coupled to the microporous member have a relatively high reaction efficiency when used to act on a substrate.

Process for preparing optically active cyanohydrins with enzymes

The reaction time and ease of handling of reactants in the known process of forming optically active (S)-cyanohydrins by reacting aldehydes with hydrocyanic acid in the presence of the enzyme S-oxynitrilase and a solvent may be significantly improved over the known methods when the solution of aldehydes and acid is passed through a porous membrane comprising a polymeric resinous binder having finely divided filler particles dispersed throughout the binder to which the S-oxynitrilase enzyme has been chemically bound.

Functional porous fibres

The invention relates to a method for the preparation of porous polymeric fibres comprising functionalized or active particles. By extruding a mixture of one or more dissolved polymers with particulate material a porous fibre is obtained in which the particulate material is entrapped. Extrusion of the fibre occurs under two-step phase inversion conditions. In particular the porous fibres can be used for the isolation of macromolecules such as peptides, proteins, nucleic acids or other organic compounds from complex reaction mixtures, in particular from fermentation broths. Another application is the immobilization of a catalyst in a reaction mixture.

Method for the preparation of functional porous fibres

Emulsion-derived particles

Polymer stabilized proteinases

Methods are provided for the stabilization of proteinases by the covalent attachment of or admixture with water-soluble polymers. The resultant stabilized proteinases have increased stability under the harsh conditions used in industrial genomics, which permits their use in the extraction and isolation of nucleic acids and the identification of disease-related prion proteins at elevated temperatures in solutions containing chaotropic agents, such as sodium dodecyl sulfate, urea or guanidinium salts, conferring advantages for robotic applications.

Preparation of enzymatically active membranes

1. A PROCESS OF PRODUCING AN ENZYMATICALLY ACTIVE MEMBRANE WHICH COMPRISES SWELLING A MEMBRANCE FORMED FROM A PROTEIN SELECTED FROM THE GROUP CONSISTING OF COLLAGEN, ZEIN, CASEIN, OVALBUMIN, WHEAT GLUTEN, FIBRINOGEN, MYSOIN, AND MUCOPROTEIN, OR A POLYPEPTIDE SELECTED FROM THE GROUP CONSISTING OF POLYGLUTAMATE, POLYASPRTATE, POLYPHENYLAMINE, POLYTRYROSINE AND COPOLYMER OF LEUCINE AND P-AMINO PHENYLANALINE TO ITS MAXIMUM CAPACITY, AND SOAKING SAID SWOLLEN MEMBRANE IN AN AQUEOUS SOLUTION OF AN ENZYME AT A TEMPERATURE OF 4-10*C. FOR A PERIOD OF 10 HOURS TO 2 DAYS UNTIL SAID ENZYME COMPLEXES BY FORMING COMPLEXING GROUPS SELECTED FROM THE GROUP CONSISTING OF MULTIPLE HYDROGEN BONDS, SALT LINKAGES AND VAN DER WAALS INTERACTIONS WITH SAID MEMBRANE TO FORM AN ENZYME MAMBRANE COMPLEX, AND THEREAFTER DYING SAID ENZYMEMEMBRANE COMPLEX.

Enteral feeding device and related methods of use

Embodiments of the disclosure are drawn to an enteral feeding device for hydrolyzing triglycerides in a nutritional formula. The device may include a body housing a chamber, an inlet configured to fluidly couple with a source of nutritional formula, and an outlet configured to fluidly couple with an enteral feeding tube. The device may include a headspace and a plurality of particles contained within the chamber, wherein the lipase is covalently bonded to the plurality of particles. The device may include an inlet filter located between the inlet and the chamber, wherein the inlet filter contains a first plurality of openings, and an outlet filter located between the chamber and the outlet, wherein the outlet filter has a second plurality of openings smaller than the plurality of particles.

The method of obtaining 6-aminopenicillanic acid

Coatings containing polymer modified enzyme for stable self-cleaning of organic stains

Bioactive coatings that are stabilized against inactivation by weathering are provided including a base associated with a chemically modified enzyme, and, optionally a first polyoxyethylene present in the base and independent of the enzyme. The coatings are optionally overlayered onto a substrate to form an active coating facilitating the removal of organic stains or organic material from food, insects, or the environment.

Non-immunogenic polypeptides

Polypeptides such as enzymes and insulin are coupled to polyethylene glycol or polypropropylene glycol having a molecular weight of 500 to 20,000 daltons to provide a physiologically active non-immunogenic water soluble polypeptide composition. The polyethylene glycol or polypropylene glycol protect the polypeptide from loss of activity and the composition can be injected into the mammalian circulatory system with substantially no immunogenic response.

Membrane carrier for immobilizing protein and its preparation

(57)【要約】本公報は電子出願前の出願データであるた め要約のデータは記録されません。

Membranes for wastewater-generated energy and gas

An example includes an apparatus, method, and system for producing and extracting a gas from a wastewater fluid. The apparatus can include a membrane including at least one hollow fiber, the at least one hollow fiber configured to convey a gas extracted from a wastewater fluid. Bacteria can be immobilized within the membrane, and the bacteria configured to produce the gas during treatment of the wastewater fluid.

Method for isolation of tetra-dimensional form of uricase and uricase

FIELD: medicine, biochemistry. SUBSTANCE: claimed method includes administration of uricase solution into at least one separation column at pH approximately 9-10,5 and reduction from the said column of one or more fractions containing isolated tetra-dimensional urecase aggregate-free uricase, wherein tetra-dimensional urecase aggregates are larger than tetra-dimensional urecase. EFFECT: 9 cl, 12 ex, 13 dwg. ÐÎÑÑÈÉÑÊÀß ÔÅÄÅÐÀÖÈß RU (19) (11) 2 278 680 (13) C2 (51) ÌÏÊ A61K 35/407 (2006.01) A61K 38/44 (2006.01) ÔÅÄÅÐÀËÜÍÀß ÑËÓÆÁÀ ÏÎ ÈÍÒÅËËÅÊÒÓÀËÜÍÎÉ ÑÎÁÑÒÂÅÍÍÎÑÒÈ, ÏÀÒÅÍÒÀÌ È ÒÎÂÀÐÍÛÌ ÇÍÀÊÀÌ (12) ÎÏÈÑÀÍÈÅ ÈÇÎÁÐÅÒÅÍÈß Ê ÏÀÒÅÍÒÓ (21), (22) Çà âêà: 2004104953/15, 02.08.1999 (24) Äàòà íà÷àëà îòñ÷åòà ñðîêà äåéñòâè ïàòåíòà: 02.08.1999 (45) Îïóáëèêîâàíî: 27.06.2006 Áþë. ¹ 18 (56) Ñïèñîê äîêóìåíòîâ, öèòèðîâàííûõ â îò÷åòå î ïîèñêå: US 3616231, 26.10.1971. US 4317878, 02.03.1982. Õðîìàòîãðàôè . Ïðàêòè÷åñêîå ïðèëîæåíèå ìåòîäà, ÷.1./Ïîä ðåä. Ý.Õåôòìàíà. - Ì.: Ìèð, 1986, ñ.109-109, 111-124. SU 421162, 25.03.1974. (54) ÑÏÎÑÎÁ ÈÇÎËÈÐÎÂÀÍÈß ÒÅÒÐÀÌÅÐÍÎÉ ÔÎÐÌÛ ÓÐÈÊÀÇÛ È ÓÐÈÊÀÇÀ, ÏÎËÓ×ÅÍÍÀß ÄÀÍÍÛÌ ÑÏÎÑÎÁÎÌ (57) Ðåôåðàò: Èçîáðåòåíèå îòíîñèòñ ê ìåäèöèíå, áèîõèìèè. Ñïîñîá âêëþ÷àåò ââåäåíèå ðàñòâîðà óðèêàçû â ïî ìåíüøåé ìåðå îäíó ñåïàðàöèîííóþ êîëîííó ïðè çíà÷åíèè ðÍ ìåæäó ïðèìåðíî 9 è ïðèìåðíî 10,5 è âîññòàíîâëåíèå èç óïîì íóòîé êîëîííû îäíîé èëè íåñêîëüêèõ ôðàêöèé, ñîäåðæàùèõ èçîëèðîâàííóþ òåòðàìåðíóþ óðèêàçó, ñâîáîäíóþ îò àãðåãàòîâ óðèêàçû, ïðè ýòîì àãðåãàòû òåòðàìåðíîé óðèêàçû áîëüøå, ÷åì òåòðàìåðíà óðèêàçà è ïåãèëèðîâàíèå óïîì íóòîé óðèêàçû. 2 í. è 7 ç.ï.ô-ëû, 12 èë., 1 òàáë. R U 2 2 7 8 6 8 0 Àäðåñ äë ïåðåïèñêè: 119333, Ìîñêâà, Ëåíèíñêèé ïð-êò, 60/2, êâ.160, ïàò.ïîâ. Â.Ï.Çûëþ C 2 C 2 (62) Íîìåð è äàòà ïîäà÷è ïåðâîíà÷àëüíîé çà âêè, èç êîòîðîé äàííà çà âêà âûäåëåíà: 2001103144 02.08.1999 Ñòðàíèöà: 1 RU 2 2 7 8 6 8 0 (73) Ïàòåíòîîáëàäàòåëü(è): ÌÀÓÍÒÝÍ ÂÜÞ ÔÀÐÌÀÑÜÞÒÈÊÝËÇ, ÈÍÊ. (US), ÄÞÊ ÞÍÈÂÅÐÑÈÒÈ (US) (43) Äàòà ïóáëèêàöèè çà âêè: 27.07.2005 R U (30) Êîíâåíöèîííûé ïðèîðèòåò: 06.08.1998 US ...

Intermediates for conjugation of polypeptides with high molecular weight polyalkylene glycols

A biologically persistent, water-soluble, substantially non-immunogenic, substantially non-antigenic conjugate of superoxide dismutase is prepared by coupling one to five strands of a polyalkylene glycol which is polyethylene glycol or polyethylene-polypropylene glycol copolymer, wherein said polyalkylene glycol has an average molecular weight of about 35,000-1,000,000.

Enzyme immobilized adhesive layer for analyte sensors

Disclosed are devices for determining an analyte concentration (e.g., glucose). The devices comprise a sensor configured to generate a signal associated with a concentration of an analyte and a sensing membrane located over the sensor. The sensing membrane comprises an enzyme layer, wherein the enzyme layer comprises an enzyme and a polymer comprising polyurethane and/or polyurea segments and one or more zwitterionic repeating units. The enzyme layer protects the enzyme and prevents it from leaching from the sensing membrane into a host or deactivating.

Electrochemical biosensor

Electrochemical biosensor consisting of a single-piece electrode comprising an anode and a cathode; a cap provided with a central perforation and a neutral membrane permitting the diffusion of substrate is attached to the end of the sensor; the biosensor contains, in the space available between the cap and the body of the electrode and adjacent to the working electrode, enzymes immobilised on particles of a polymer which is non-soluble in the reaction medium. Use for the assay of organic substrates liberating hydrogen peroxide by enzymatic reaction.

Enzyme immobilized adhesive layer for analyte sensors

Disclosed are devices for determining an analyte concentration (e.g., glucose). The devices comprise a sensor configured to generate a signal associated with a concentration of an analyte and a sensing membrane located over the sensor. The sensing membrane comprises an enzyme layer, wherein the enzyme layer comprises an enzyme and a polymer comprising polyurethane and/or polyurea segments and one or more zwitterionic repeating units. The enzyme layer protects the enzyme and prevents it from leaching from the sensing membrane into a host or deactivating.

Membrane layers for analyte sensors

Disclosed are devices for determining an analyte concentration (e.g., glucose). The devices comprise a sensor configured to generate a signal associated with a concentration of an analyte and a sensing membrane located over the sensor. The sensing membrane comprises a biointerface layer which interfaces with a biological fluid containing the analyte to be measured. The biointerface layer can comprises a biointerface polymer, wherein the biointerface polymer comprises polyurethane and/or polyurea segments and one or more zwitterionic repeating units. The sensing membrane can also comprise an enzyme layer, wherein the enzyme layer comprises an enzyme and a polymer comprising polyurethane and/or polyurea segments and one or more zwitterionic repeating units. The sensing membrane can also comprise a diffusion-resistance layer, which can comprise a base polymer having a lowest Tg of greater than −50 C.

Membrane layers for analyte sensors

Disclosed are devices for determining an analyte concentration (e.g., glucose). The devices comprise a sensor configured to generate a signal associated with a concentration of an analyte and a sensing membrane located over the sensor. The sensing membrane comprises a biointerface layer which interfaces with a biological fluid containing the analyte to be measured. The biointerface layer can comprises a biointerface polymer, wherein the biointerface polymer comprises polyurethane and/or polyurea segments and one or more zwitterionic repeating units. The sensing membrane can also comprise an enzyme layer, wherein the enzyme layer comprises an enzyme and a polymer comprising polyurethane and/or polyurea segments and one or more zwitterionic repeating units. The sensing membrane can also comprise a diffusion-resistance layer, which can comprise a base polymer having a lowest Tg of greater than -50C. II I0onni U!99O uJ , C/) I-U

Membrane layers for analyte sensors

Disclosed are devices for determining an analyte concentration (e.g., glucose). The devices comprise a sensor configured to generate a signal associated with a concentration of an analyte and a sensing membrane located over the sensor. The sensing membrane comprises a biointerface layer which interfaces with a biological fluid containing the analyte to be measured. The biointerface layer can comprises a biointerface polymer, wherein the biointerface polymer comprises polyurethane and/or polyurea segments and one or more zwitterionic repeating units. The sensing membrane can also comprise an enzyme layer, wherein the enzyme layer comprises an enzyme and a polymer comprising polyurethane and/or polyurea segments and one or more zwitterionic repeating units. The sensing membrane can also comprise a diffusion-resistance layer, which can comprise a base polymer having a lowest Tg of greater than -50C.

A GLUCOSE SENSOR.

SENSOR (10) CAPAZ DE MEDIR CON PRECISION LA GLUCOSA Y OTRAS MATERIAS A ANALIZAR EN AMBIENTES POBRES EN OXIGENO. EL SENSOR UTILIZA UNA MEMBRANA (14) QUE CONTIENE UNA ENZIMA, FORMADA DE UNA RED POLIMERA SEMI - INTERPENETRANTE INFILTRADO POR ESTA ENZIMA. LA MEMBRANA QUE CONTIENE LA ENZIMA INCREMENTA EL TRANSPORTE DE OXIGENO HACIA LA ENZIMA DE FORMA QUE EL OXIGENO NO CONSTITUYA UN FACTOR DE LIMITACION PARA LA OXIDACION QUE SE PRODUCE A NIVEL DE LA ENZIMA.

Peg-urate oxidase conjugates, their application, method of tetrameric uricase form separation

FIELD: medicine, pharmaceutics. SUBSTANCE: invention concerns biopharmaceutics and PEG conjugates of natural or recombinant urate oxidase (uricase). Uricase is bound covalently to poly(ethylene glycol) or poly(ethylene oxide) (both denoted as PEG), with average of 2 to 10 PEG threads are conjugated with each uricase sub-unit, and average molecular weight of PEG is approximately between 5 kDa and 100 kDa. EFFECT: obtaining almost non-immunogenic PEG uricase conjugates preserving at least 75% of uricolytic activity of non-modified enzyme. 44 cl, 12 ex, 17 dwg ÐÎÑÑÈÉÑÊÀß ÔÅÄÅÐÀÖÈß RU (19) (11) 2 349 341 (13) C2 (51) ÌÏÊ A61K 38/44 (2006.01) A61K 35/407 (2006.01) A61K 47/48 (2006.01) ÔÅÄÅÐÀËÜÍÀß ÑËÓÆÁÀ ÏÎ ÈÍÒÅËËÅÊÒÓÀËÜÍÎÉ ÑÎÁÑÒÂÅÍÍÎÑÒÈ, ÏÀÒÅÍÒÀÌ È ÒÎÂÀÐÍÛÌ ÇÍÀÊÀÌ (12) ÎÏÈÑÀÍÈÅ ÈÇÎÁÐÅÒÅÍÈß Ê ÏÀÒÅÍÒÓ (21), (22) Çà âêà: 2006107111/15, 09.03.2006 (24) Äàòà íà÷àëà îòñ÷åòà ñðîêà äåéñòâè ïàòåíòà: 02.08.1999 (30) Êîíâåíöèîííûé ïðèîðèòåò: 06.08.1998 US 09/130392 (45) Îïóáëèêîâàíî: 20.03.2009 Áþë. ¹ 8 (56) Ñïèñîê äîêóìåíòîâ, öèòèðîâàííûõ â îò÷åòå î ïîèñêå: US 4179337 À, 18.12.1997. RU 94006023 À1, 20.04.1996. RU 2105812 C1, 27.02.1998. (62) Íîìåð è äàòà ïîäà÷è ïåðâîíà÷àëüíîé çà âêè, èç êîòîðîé äàííà çà âêà âûäåëåíà: 2004104953 16.02.2004 ÒÅÒÐÀÌÅÐÍÎÉ ÔÎÐÌÛ ÓÐÈÊÀÇÛ (57) Ðåôåðàò: Èçîáðåòåíèå îòíîñèòñ ê áèîôàðìàêîëîãèè è êàñàåòñ PEG-êîíúþãàòîâ âñòðå÷àþùåéñ â ïðèðîäå èëè ðåêîìáèíàíòíîé óðàòîêñèäàçû (óðèêàçû). Óðèêàçà êîâàëåíòíî ñâ çûâàåòñ ñ ïîëè(ýòèëåíãëèêîëåì) èëè ïîëè(ýòèëåíîêñèäîì) (îáà îáîçíà÷àþòñ PEG), ïðè÷åì â ñðåäíåì îò 2 äî 10 íèòåé PEG êîíúþãèðóþò ñ êàæäîé ïîäúåäèíèöåé óðèêàçû, è PEG èìååò ñðåäíèé ìîëåêóë ðíûé âåñ ìåæäó ïðèìåðíî 5 è 100 êÄà. Ïîëó÷åííûå PEGóðèêàçíûå êîíúþãàòû âë þòñ ïðàêòè÷åñêè íåèììóíîãåííûìè è ñîõðàí þò ïî ìåíüøåé ìåðå 75% óðèêîëèòè÷åñêîé àêòèâíîñòè íåìîäèôèöèðîâàííîãî ôåðìåíòà. 3 í. è 41 ç.ï. ôëû, 17 èë. R U 2 3 4 9 3 4 1 (54) ÊÎÍÚÞÃÀÒÛ PEG-ÓÐÀÒÎÊÑÈÄÀÇÛ, ÈÕ ÈÑÏÎËÜÇÎÂÀÍÈÅ, ÑÏÎÑÎÁ ÈÇÎËÈÐÎÂÀÍÈß Ñòðàíèöà: 1 RU C 2 C 2 Àäðåñ äë ïåðåïèñêè: 127055, Ìîñêâà, à/ 11, ...

Modified hyaluronidases and uses in treating hyaluronan-associated diseases and conditions

Provided are combinations, compositions and kits containing a hyaluronan degrading enzyme, such as a soluble hyaluronidase, for treatment of hyaluronan-associated conditions, diseases and disorders. In one example, the products include an additional agent or treatment. Such products can be used in methods for administering the products to treat the hyaluronan-associated diseases and conditions, for example, hyaluronan-associated cancers, for example, hyaluronan-rich tumors. The methods include administration of the hyaluronan degrading enzyme composition alone or in combination with other treatments. Also provided are methods and compositions for providing sustained treatment effects in hyaluronan-associated diseases and conditions.

Non-antigenic branched polymer conjugates

Branched, substantially non-antigenic polymers are disclosed. Conjugates prepared with the polymers and biologically active molecules such as proteins and peptides demonstrate extended circulating life in vivo. Substantially fewer sites on the biologically active material are used as attachment sites. Methods of forming the polymer, conjugating the polymers with biologically active moieties and methods of using the conjugates are also disclosed.

Modified hyaluronidases for use in treating hyaluronan-associated diseases and conditions

Provided are combinations, compositions and kits containing a hyaluronan degrading enzyme, such as a soluble hyaluronidase, for treatment of hyaluronan-associated conditions, diseases and disorders. In one example, the products include an additional agent or treatment. Such products can be used in methods for administering the products to treat the hyaluronan-associated diseases and conditions, for example, hyaluronan-associated cancers, for example, hyaluronan-rich tumors. The methods include administration of the hyaluronan degrading enzyme composition alone or in combination with other treatments. Also provided are methods and compositions for providing sustained treatment effects in hyaluronan-associated diseases and conditions.

Modified hyaluronidases and uses in treating hyaluronan-associated diseases and conditions

Provided are combinations, compositions and kits containing a hyaluronan degrading enzyme, such as a soluble hyaluronidase, for treatment of hyaluronan-associated conditions, diseases and disorders. In one example, the products include an additional agent or treatment. Such products can be used in methods for administering the products to treat the hyaluronan-associated diseases and conditions, for example, hyaluronan-associated cancers, for example, hyaluronan-rich tumors. The methods include administration of the hyaluronan degrading enzyme composition alone or in combination with other treatments. Also provided are methods and compositions for providing sustained treatment effects in hyaluronan-associated diseases and conditions.

Uricase peg-conjugates and their applying

FIELD: biochemistry, pharmaceutical chemistry. SUBSTANCE: invention relates to preparing conjugate of naturally occurring or recombinant urate oxidase (uricase) bound covalently with poly-(ethylene glycol) or poly-(ethylene oxide) (both are designated as PEG) wherein in average from 4 to 10 PEG strands are conjugated with each subunit of uricase and molecular mass of PEG is about between 20 and 40 kDa. Prepared PEG-uricase conjugates are nonimmunogenic practically and retain at least 75% of uricolytic activity of nonmodified enzyme. EFFECT: improved preparing method, valuable properties of conjugates. 22 cl, 17 dwg, 12 ex ÐÎÑÑÈÉÑÊÀß ÔÅÄÅÐÀÖÈß (19) RU (51) ÌÏÊ 7 (11) (13) 2 246 318 C2 A 61 K 47/48, 38/44 ÔÅÄÅÐÀËÜÍÀß ÑËÓÆÁÀ ÏÎ ÈÍÒÅËËÅÊÒÓÀËÜÍÎÉ ÑÎÁÑÒÂÅÍÍÎÑÒÈ, ÏÀÒÅÍÒÀÌ È ÒÎÂÀÐÍÛÌ ÇÍÀÊÀÌ (12) ÎÏÈÑÀÍÈÅ ÈÇÎÁÐÅÒÅÍÈß Ê ÏÀÒÅÍÒÓ (21), (22) Çà âêà: 2001103144/15, 02.08.1999 (24) Äàòà íà÷àëà äåéñòâè ïàòåíòà: 02.08.1999 (30) Ïðèîðèòåò: 06.08.1998 US 09/130,392 (43) Äàòà ïóáëèêàöèè çà âêè: 20.10.2003 (45) Îïóáëèêîâàíî: 20.02.2005 Áþë. ¹ 5 (85) Äàòà ïåðåâîäà çà âêè PCT íà íàöèîíàëüíóþ ôàçó: 06.03.2001 (86) Çà âêà PCT: US 99/17514 (02.08.1999) (54) ÊÎÍÚÞÃÀÒÛ PEG-ÓÐÈÊÀÇÛ È ÈÕ ÈÑÏÎËÜÇÎÂÀÍÈÅ (57) Ðåôåðàò: PEG èìååò ñðåäíèé ìîëåêóë ðíûé âåñ ìåæäó Èçîáðåòåíèå îòíîñèòñ ê ôàðìàöåâòè÷åñêîé ïðèìåðíî 20 êÄà è 40 êÄà. Ïîëó÷åííûå õèìèè. Âñòðå÷àþùà ñ â ïðèðîäå èëè PEG-óðèêàçíûå êîíúþãàòû âë þòñ ïðàêòè÷åñêè ðåêîìáèíàíòíà óðàòîêñèäàçà (óðèêàçà) êîâàëåíòíî íåèììóíîãåííûìè è ñîõðàí þò ïî ìåíüøåé ìåðå ñâ çûâàåòñ ñ ïîëè(ýòèëåí ãëèêîëåì) èëè 75% óðèêîëèòè÷åñêîé àêòèâíîñòè ïîëè(ýòèëåí îêñèäîì) (îáà îáîçíà÷àþòñ PEG), íåìîäèôèöèðîâàííîãî ôåðìåíòà. 2 í. è 20 ç.ï. ïðè÷åì â ñðåäíåì îò 4 äî 10 íèòåé PEG ô-ëû, 17 èë., 1 òàáë. êîíúþãèðóþò ñ êàæäîé ïîäúåäèíèöåé óðèêàçû, è R U 2 2 4 6 3 1 8 Àäðåñ äë ïåðåïèñêè: 119333, Ìîñêâà, Ëåíèíñêèé ïð-êò, 60/2, êâ.160, ïàò.ïîâ. Â.Ï.Çûëþ Ñòðàíèöà: 1 RU C 2 C 2 (87) Ïóáëèêàöè PCT: WO 00/07629 (17.02.2000) 2 2 4 6 3 1 8 (56) Ñïèñîê äîêóìåíòîâ, öèòèðîâàííûõ â îò÷åòå î ïîèñêå: US ...

BIOLOGICALLY ACTIVE VID AFFINITETSREAKTIONER ANVAENDBART ADSORBTIONSMEDEL

Process for the preparation of carrier-bound proteins

Polymeric carrier bound ligands

A process for bonding a ligand to a carrier through the use of a pyrimidine derivative of the formula: ##STR1## in which R 1 , R 2 , R 3 and R 4 are each independently hydrogen, hydroxyl, halogen, lower alkyl, lower alkoxy, loweralkylthio, loweracylamino, nitro, cyano, carboxamido, loweralkylsulphonyl, loweralkoxycarbonyl, phenyl, trifluoromethyl or chloromethyl, the ligand or the carrier containing at least one functional group that reacts with the pyramidine derivative.

Immobilized enzymes and uses thereof

The present invention generally relates to uses of immobilized enzymes. Immobilized enzymes can be used for various chemical transformations, separations, and purifications and can be used in sensors and diagnostics

Nano-micro hybridfiber-based cell culture construct and assay chip comprising the same

The present invention relates to a nano-micro hybrid fiber-based three-dimensional cell culturing structure and an assay chip including the same. More specifically, the present invention relates to a nano-micro hybrid fiber-based cell culturing structure, an assay chip including the same, and a method for analyzing cells using the assay chip, wherein the cell culturing structure includes a cell culturing support for adhering and culturing one or more cells, and the support is formed in a three-dimensional mat shape made of hybrid fibers of nano fibers and micro fibers, so that the cells are permeated and adhered in the support. The nano-micro hybrid fiber-based cell culturing structure according to the present invention can simultaneously and three-dimensionally culture one or more cells. Accordingly, the cell culturing structure according to the present invention can be used as an assay chip which can measure and analyze the permeation and adhesion of the cells, the shape of the cells, the connection between the cells, or the movement between the cells. Moreover, using the assay chip according to the present invention, the interaction and bonding of various immunity cells and disease cells can be measured in real time, so that the cells can be three-dimensionally analyzed in real time.

A kind of enzyme process acid stripping method of partial glyceride lipase and the grease rich in PUFA

本发明公开了一种偏甘油酯脂肪酶及富含PUFA的油脂的酶法脱酸方法，包括以下步骤：1)将富含PUFA的油脂与非极性有机溶剂、短链一元醇混合，添加固定化偏甘油酯脂肪酶进行酯化反应；所述偏甘油酯脂肪酶是将Lipase SMG1第278位的Phe突变为Asn后得到的突变体；2)回收固定化酶，回收有机溶剂和一元醇，即得到脱酸后的富含PUFA的油脂。本发明所使用的偏甘油酯脂肪酶不催化甘油三酯发生醇解等副反应，脱酸效率高，反应温度低，避免了PUFA的高温氧化，且固定化酶可多次回收重复利用，在工业上具有良好的应用前景。

Method for immobilizing a biologic in a polyurethane-hydrogel composition, a composition prepared from the method, and biomedical applications

A biologic can be immobilized in a transparent polyurethane-hydrogel composition. Such a polyurethane-hydrogel composition can be prepared from forming a polyurethane-hydrogel mixture and immobilizing a biologic in the mixture. The mixture can be formed by admixing a prepolymer and a water-soluble crosslinker in aqueous solvent and in the substantial absence of organic solvent. A suitable prepolymer generally includes at least one water-soluble polyol and at least one isocyanate and can be added to the mixture in an amount of no greater than about 5 weight percent based on total weight of all components. a suitable crosslinker generally has a crosslinker functionality of at least 2. A biologic includes cells, peptides, nucleics, peptide nucleic acids, saccharides, lipopolysaccharides, glycolipids, and combinations of these. A polyurethane-hydrogel composition having an immobilized biologic can be particularly useful for biomedical applications. Some examples of useful biomedical applications include protein microarrays, cell microarrays, and DNA microarrays.

Method for hydrolyzing and splitting 2-phenylbutyric acid enantiomer by using immobilized lipase as catalyst

本发明属于生物催化技术领域，公开了一种固定化脂肪酶催化酯水解拆分2‑苯基丁酸对映体的方法，利用共价连接法将利用大分子修饰剂修饰后的南极假丝假丝酵母脂肪酶(CAL‑A)与载体UiO‑66结合，制备新型的固定化酶，以立体选择性催化2‑苯基丁酸酯水解反应得到(S)‑2‑苯基丁酸和(R)‑2‑苯基丁酸酯。与游离酶相比，固定化酶在相同条件下显著提高了酶的催化活性，具备更高的立体选择性，此外，固定化酶具有高的热稳定性，对pH耐受度，便于循环使用，容易与产物分离，显著降低了技术成本，而且操作简便，绿色环保。

Patent JPS4986587A

Glass composite materials containing alkoxosilane derivative having alterable charge, hydrophobic and hydrophilic groups

A porous glass composite material is prepared comprising a gel containing water and a polymeric network containing at least one alkoxosilane derivative having a group of alterable charge, a hydrophobic group and a hydrophilic group. The alkoxosilane derivative is preferably a derivative of an alkoxosilane having the general formula (OR 1 ) 3 Si-spacer-Si(OR 2 ) 3 , wherein R 1 and R 2 are the same or different and may be hydrogen; substituted and unsubstituted, branched and unbranched C 1-20 -alkyls; substituted and unsubstituted, branched and unbranched C 1-20 -alkenyls; substituted and unsubstituted, branched and unbranched C 1-20 -alkynyls; substituted, unsubstituted, and multiple ring aryl groups; or combinations thereof; and water. Devices including the glass composite include chromatographic and other separation media, drug delivery vehicles, and electric and mechanical actuators.

Temperature-responsive interpolymer complex

Process for producing water-soluble conjugation of c@@@,-z@@@-dependent peroxide-dismutase

Использование: медицина, получение конъюгатов пероксид-дисмутазы с полиалкиленгликолем . Сущность изобретени : фермент св зывают через аминогруппу лизина и СО-св зь с полиэтиленгликолем или изопропоксиполиэтиленгликолем с мол.м. 40000-200000, предпочтительно 40000- 150000, одна концева  группа которого модифицирована в карбоксильную, амино-, карбоксамидо- или альдегидную группу. Дл  св зывани  одной молекулы фермента берут 1-8, предпочтительно 2-6, молекул полимера. 2 з.п.ф-лы, 1 табл.

Surfactant-lipase complex immobilized on insoluble matrix

불용성 기질과 불활성 기질에 고정된 계면활성제로 코팅된 리파아제 복합물을 구성하는 리파아제 제형. Lipase formulation comprising a lipase complex coated with a surfactant immobilized on an insoluble substrate and an inert substrate. 제조방법과 새로운 생성물의 용법이 밝혀졌다. The method of preparation and the use of the new product were found.

Enzyme immobilization for use in biofuel cells and sensors

Disclosed are bioanodes comprising a quaternary ammonium treated Nafion® polymer membrane and a dehydrogenase incorporated within the treated Nafion® polymer. The dehydrogenase catalyzes the oxidation of an organic fuel and reduces an adenine dinucleotide. The ion conducting polymer membrane lies juxtaposed to a polymethylene green redox polymer membrane, which serves to electro-oxidize the reduced adenine dinucleotide. The bioanode is used in a fuel cell to produce high power densities.

Direct electron transfer using enzymes in bioanodes, biocathodes, and biofuel cells

Bioanodes, biocathodes, and biofuel cells comprising an electron conductor, at least one anode enzyme or cathode enzyme, and an enzyme immobilization material. The anode enzyme is capable of reacting with a fuel fluid to produce an oxidized form of the fuel fluid, and capable of releasing electrons to the electron conductor. The cathode enzyme is capable of reacting with an oxidant to produce water, and capable of gaining electrons from the electron conductor. The enzyme immobilization material for both the anode enzyme and the cathode enzyme is capable of immobilizing and stabilizing the enzyme, and is permeable to the fuel fluid and/or the oxidant.

Direct electron transfer using enzymes in bioanodes, biocathodes, and biofuel cells

Bioanodes, biocathodes, and biofuel cells comprising an electron conductor, at least one anode enzyme or cathode enzyme, and an enzyme immobilization material. The anode enzyme is capable of reacting with a fuel fluid to produce an oxidized form of the fuel fluid, and capable of releasing electrons to the electron conductor. The cathode enzyme is capable of reacting with an oxidant to produce water, and capable of gaining electrons from the electron conductor. The enzyme immobilization material for both the anode enzyme and the cathode enzyme is capable of immobilizing and stabilizing the enzyme, and is permeable to the fuel fluid and/or the oxidant.

METHOD FOR MANUFACTURING A CONDUCTIVE FILM OF AN ELECTROCHEMICAL BIOREACTOR

Immobilized enzymes and uses thereof

Disclosed are bioanodes comprising a quaternary ammonium treated Nafion® polymer membrane and a dehydrogenase incorporated within the treated Nafion® polymer. The dehydrogenase catalyzes the oxidation of an organic fuel and reduces an adenine dinucleotide. The ion conducting polymer membrane lies juxtaposed to a polymethylene green redox polymer membrane, which serves to electro-oxidize the reduced adenine dinucleotide. The bioanode is used in a fuel cell to produce high power densities.

Immobilization of chemical species in crosslinked matrices

A chemical specie is immobilized in a three dimensional, crosslinked matrix by bringing together in covalent bonding proximity a desired chemical specie and a polymeric coupling compound such as a photoderivatized polymer having at least two latent photochemical reactive groups per molecule, each latent reactive group being capable when activated of covalently bonding to another coupling compound molecule or to the chemical specie. The chemical specie may be a protein, carbohydrate, nucleic acid or lipid, and desirably is free of latent reactive groups that are activated upon activation of the latent reactive groups of the coupling compound. The latent reactive groups are simultaneously activated to cause formation via covalent bonding of a three-dimensional molecular network in which molecules of the chemical specie are covalently bonded to molecules of the coupling compound, and molecules of the coupling compound are covalently bonded to each other. The matrix containing the covalently bonded chemical specie may be contacted with a different chemical specie under conditions to incorporate the specie into the matrix in unbound form so that it can be gradually released from the matrix. In a preferred embodiment, the covelently bonded chemical specis is an antithrombic agent and the inbound chemical specil is an antibiotic. Preferably, the three dimensional matrix is formed as a coating upon a surface to which covalent bonds are formed upon activation of the latent reactive groups of the coupling compound.

Process for providing enzyme activity to a surface of an article and an article having enzyme activity on a surface thereof

A process for providing enzyme activity to the surface of an article is described, comprising forming a film on the surface of the article, said film comprising an acid anhydride group-containing polymer and a polyol, wherein the acid anhydride groups are partially reacted with the polyol, and thereafter reacting the unreacted acid anhydride groups with an enzyme. Such an article provided with enzyme activity on the surface can be used for the production of food, medicines, etc., and as a medical material eg. a blood purification adsorbent, an artificial organ (heart, lung, kidney), a surgical drain.

Modified hyaluronidase and use in the treatment of hyaluronan related diseases and conditions

Support matrices for immobilized enzymes

Support matrices for immobilized enzymes comprise a combined organic-inorganic support consisting of a porous inorganic support containing an organic polymeric material which possesses functionalized pendent groups, said polymeric material being entrapped and also adsorbed in part in the pores of the inorganic porous support. The organic polymeric component of the matrix constitutes a copolymer prepared in situ from a first polyfunctional monomer, a low molecular weight polymer, a low molecular weight polymer hydrolysate, or a preformed polymer of natural or synthetic origin which is reacted with an excess of a second bifunctional reactive monomer.

METHOD OF USING AN ENZYME TO TRANSFORM, BY ENZYMATIC REACTION, AN ORGANIC SUBSTANCE INTO AT LEAST ONE OTHER ORGANIC SUBSTANCE

Enzyme immobilization for use in biofuel cells and sensors

4차 암모늄으로 처리된 나피온? 중합체 막, 및 상기 나피온? 중합체 내에 혼입된 디히드로게나제를 포함하는 바이오애노드가 개시된다. 디히드로게나제는 유기 연료의 산화를 촉매하고, 아데닌 디뉴클레오티드를 환원시킨다. 이온 전도성 중합체 막은 폴리메틸렌 그린 산화환원 중합체 막에 병렬로 위치하고 있으며, 이는 환원된 아데닌 디뉴클레오티드를 전기적으로 산화시킨다. 바이오애노드는 연료 전지 내에서 사용되어 높은 전력 밀도를 생성한다. Nafion treated with quaternary ammonium? Polymer membrane, and the Nafion? A bioanode comprising a dehydrogenase incorporated into a polymer is disclosed. Dehydrogenase catalyzes the oxidation of organic fuels and reduces adenine dinucleotides. The ion conductive polymer membrane is located in parallel to the polymethylene green redox polymer membrane, which electrically oxidizes the reduced adenine dinucleotide. Bioanodes are used in fuel cells to produce high power densities.

Penicillin G Amidase, Glutaryl-7-ACA Acylase or D-Amino Acid Oxidase Fixed on Substrate

본 발명은, 공유결합에 의해 아미노-치환된 유기실록산 중합체로 만들어진 기질에 고정되는, 페니실린 G 아미다아제 (E.C.3.5.1.11), 글루타릴-7-ACA 아실라아제 및 d-아미노산 산화효소 (E.C.1.4.3.3)로 이루어진 그룹으로부터 선택되는 담체상에 고정되는 효소에 관한 것이다. 부가하여, 본 발명은 담체상에 고정되는 효소의 체적 활성도를 개선시키는 방법에 관한 것이다.

COMBINATIONS WITH BIOLOGICAL ACTIVITY, THEIR PREPARATION PROCESS

Enzyme-attachment to silicon-contg - materials using activating agents

Enzymes are insolubilised by combination via an amide group with an organosilane, itself linked to a Si-containing carrier. The enzyme is pref. trypsin, the silane an w-aminoalkyltrialkoxysilane (esp. p-aminpropyltrimethoxysilane) and the carrier granular SiO2. The linkage is formed between -COOH in the enzyme and -NH2 in the silane, pref. under the influence of a "carboxyl activator", esp. 1-Et-3-(3-dimethylaminopropyl)-carbodiimide, a carbodiimidozole, or an iso-oxazolium salt unsubstd. in the 3-position.

PROCESS FOR THE PREPARATION OF 2-HYDROXY-4-METHYLTHIO-BUTYRIC ACID USING A NITRILASE

Patent FR2140541A1

PROCESS FOR PREPARING A CARRIER FOR IMMOBILIZATION OF A CELL, SUCH A CARRIER AND USES THEREOF

La présente invention concerne un procédé de préparation d'un support solide susceptible d'immobiliser au moins une cellule et/ou au moins une partie de cellule, ledit procédé comprenant une étape consistant à fixer audit support solide un composé fusogène capable de s'insérer dans les membranes cellulaires. La présente invention concerne également un procédé pour immobiliser au moins une cellule et/ou au moins une partie de cellule utilisant le support solide ainsi préparé, ledit support solide et ses utilisations dans le domaine du diagnostic biomédical ou de la surveillance sanitaire de fluides biologiques ou destinés à l'usage humain ou animal. The present invention relates to a method for preparing a solid support capable of immobilizing at least one cell and / or at least one cell part, said method comprising a step of fixing to said solid support a fusogenic compound capable of inserting in cell membranes. The present invention also relates to a method for immobilizing at least one cell and / or at least one cell part using the solid support thus prepared, said solid support and its uses in the field of biomedical diagnosis or health monitoring of biological fluids or intended for human or animal use.

PROCESS FOR THE PREPARATION OF 2-HYDROXY-4-METHYLTHIO-BUTYRIC ACID USING NITRILASE

The invention concerns a method for preparing 2-hydroxy 4-methylthio butyric acid and/or the ammonium salt of 2-hydroxy 4-methylthio butyric acid by enzymatic hydrolysis of 2-hydroxy 4-methythio butyronitrile characterised in that: a) in a first step a biological material having nitrilase activity is prepared, b) in a second step it is immobilised, c) in a third step, 2-hydroxy 4-methylthio butyronitrile is brought in the presence of the immobilised biological material, for obtaining the ammonium salt of 2-hydroxy 4-methylthio butyric acid, d) in a fourth step, optionally, the salt obtained in step c) is transformed into the corresponding acid, and e) in a fifth step, the product resulting from step c) or d) is concentrated.

METHOD FOR DEPOSITING NANOBJETS ON THE SURFACE OF A POLYMERIC GEL OF UNIFORM RIGIDITY

L'invention concerne un procédé de dépôt de nanoobjets sur la surface d'un gel comprenant les étapes de : a) fournir un gel comprenant une matrice polymérique et un solvant au sein de la matrice polymérique, la matrice polymérique formant un réseau tridimensionnel susceptible de gonfler en présence dudit solvant, où la solubilité de la matrice polymérique à 1 bar et 25°C dans le solvant est inférieure à 1 g/L, où le gel a un gradient de rigidité à l'échelle du micromètre inférieur à 10%, puis b) déposer des nanoobjets à la surface du gel, lesdits nanoobjets ayant un diamètre moyen supérieur ou égal au diamètre moyen des pores du gel, puis c) évaporer le solvant du gel au moins jusqu'à ce que la teneur en solvant ne varie plus avec le temps, sous réserve qu'au début de l'évaporation, la teneur en sels minéraux dans le solvant soit inférieure à 6 g/L, le gel susceptible d'être obtenu et ses utilisations. The invention relates to a method for depositing nanoobjects on the surface of a gel comprising the steps of: a) providing a gel comprising a polymeric matrix and a solvent within the polymeric matrix, the polymeric matrix forming a three-dimensional network capable of swelling in the presence of said solvent, wherein the solubility of the polymer matrix at 1 bar and 25 ° C in the solvent is less than 1 g / L, where the gel has a micrometer-scale stiffness gradient of less than 10%, and then b) depositing nanoobjects on the surface of the gel, said nanoobjects having an average diameter greater than or equal to the average pore diameter of the gel, and then c) evaporating the solvent from the gel at least until the solvent content does not vary. more over time, provided that at the beginning of the evaporation, the content of mineral salts in the solvent is less than 6 g / L, the gel that can be obtained and its uses.

Biosensors produced from enzymes with reduced solubility and methods of production and use thereof

용해도를 감소시키기 위해 변형된 효소를 포함하는 다중-용도 바이오센서가 개시되며; 상기 다중-용도 바이오센서는 유체 생물학적 샘플에서 분석물을 검출하는 데 사용되고, 바이오센서는 또한 많은 사용 후에도 이의 효소 활성을 유지한다. 다수의 바이오센서를 포함하는 다중-센서 어레이가 개시된다. 이들 장치를 생성시키고 이용하는 방법이 또한 개시된다. A multi-use biosensor comprising an enzyme modified to reduce solubility is disclosed; The multi-use biosensor is used to detect an analyte in a fluid biological sample, and the biosensor also retains its enzymatic activity after many uses. A multi-sensor array comprising a plurality of biosensors is disclosed. Methods of making and using these devices are also disclosed.

Nanozymes, methods of making nanozymes, and methods of using nanozymes