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Небесная энциклопедия

Космические корабли и станции, автоматические КА и методы их проектирования, бортовые комплексы управления, системы и средства жизнеобеспечения, особенности технологии производства ракетно-космических систем

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Мониторинг СМИ

Мониторинг СМИ и социальных сетей. Сканирование интернета, новостных сайтов, специализированных контентных площадок на базе мессенджеров. Гибкие настройки фильтров и первоначальных источников.

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Поддерживает ввод нескольких поисковых фраз (по одной на строку). При поиске обеспечивает поддержку морфологии русского и английского языка
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Применить Всего найдено 108946. Отображено 100.
05-01-2012 дата публикации

Apparatus and methods for enzymatic debridement of skin lesions

Номер: US20120004627A1
Автор: Amihay Freeman, Eran Hirszowicz, Michal Be'eri-Lipperman
Принадлежит: EnzySurge Ltd, Ramot at Tel Aviv University Ltd

An apparatus for debridement of devitalized tissue in skin lesions, that includes a plurality of height- and angle-adjustable inlet tubes and at least one outlet tube and a member that forms an occlusive seal around a skin lesion. The plurality of inlet tubes is adapted for directing a continuous stream of enzymatic solution to the surface and into the entire volume of the wound bed of the lesion and the at least one outlet is adapted for removing the enzymatic solution, fluids draining from the lesion and tissue debris from the occluded skin lesion.

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Номер записи: 1
12-01-2012 дата публикации

Use of Molecular Chaperones for the Enhanced Production of Secreted, Recombinant Proteins in Mammalian Cells

Номер: US20120009670A1
Автор: Hsinyi Yvette Tang, Ruth Kelly, Sham-Yuen Chan, Yiwen Tao, Yongjian Wu
Принадлежит: Bayer Pharmaceuticals Corp

The present invention relates to a method for increased production of a secreted, recombinant protein product through the introduction of molecular chaperones in a mammalian host cell. The present invention also relates to a mammalian host cell with enhanced expression of a secreted recombinant protein product by coexpressing at least one chaperone protein.

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Номер записи: 2
19-01-2012 дата публикации

Anti-polyethylene glycol antibody expressing cell quantify any free polyethylene glycol and polyethylene glycol-derivatized molecules

Номер: US20120015380A1
Автор: Kuo-Hsiang Chuang, Ssu-Jung Lu, Steven R. Roffler, Tian-Lu Cheng
Принадлежит: KAOHSIUNG MEDICAL UNIVERSITY

In this invention, anti-PEG antibodies or anti-methoxyl-PEG (anti-CH 3 O-PEG) antibodies were expressed on cell surface which can collocate with a biotinylated anti-PEG antibody (AGP4-Biotin) or biotinylated PEG (PEG-Biotin) to develop a cell-based sandwich ELISA or a cell-based competition ELISA, respectively. Both of these two methods could sensitively quantify free PEG and PEG-modified macromolecules (proteins, nanoparticles and liposomes) as sensitive as nano-gram level.

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Номер записи: 3
26-01-2012 дата публикации

MHC-Less cells

Номер: US20120020885A1
Автор: Edward K.Y. Jung, Elizabeth A. Sweeney, Eric C. Leuthardt, Gary L. McKnight, Lowell L. Wood, JR., Muriel Y. Ishikawa, Roderick A. Hyde, Stephen L. Malaska, Wayne R. Kindsvogel
Принадлежит: SEARETE LLC

The present disclosure relates to compositions, methods, systems, computer-implemented methods, and computer program products thereof that relate to biological cells for delivery of at least one therapeutic agent to a biological tissue or subject.

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Номер записи: 4
26-01-2012 дата публикации

Method for improved single cell cloning

Номер: US20120021510A1
Автор: Aziz Cayli, Kolja Hegel, Olaf KRÜGER
Принадлежит: Cellca GmbH

The present invention relates to methods for the cultivation of a population of cells in a serum free cell culture medium, wherein the population of cells has a cell concentration of less than 100 cells/ml, wherein a serum free cell culture medium containing recombinant albumin and recombinant transferrin is used.

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Номер записи: 5
02-02-2012 дата публикации

Method of using stem cells to aid in diagnosis

Номер: US20120027678A1
Автор: Albert Scheller, William C. Rader
Принадлежит: Individual

The present invention provides a method for the in vitro culture of embryonic stem cells, wherein the stem cells continue to express no antigen or antigen CD117, and mostly remain undifferentiated during culture. The present invention also relates to purified preparations of embryonic stem cells and for uses of embryonic stem cells in treating a wide variety of conditions, diseases and disorders.

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Номер записи: 6
09-02-2012 дата публикации

Pharmaceutical composition for treating or preventing cancer by inducing dendritic cell-like differentiation from monocytes to improve anticancer immune activity

Номер: US20120034251A1
Автор: Hiromi Kumon, Masami Watanabe, Yasutomo Nasu, Yuji Kashiwakura
Принадлежит: Momotaro Gene Inc

According to the present invention, a composition for inducing or activating dendritic cell-like cells so as to treat or prevent cancer by immunotherapy is provided. Specifically, the following is provided: an agent for activating cancer immunity, which comprises, as an active ingredient, the following REIC protein: (a) a protein consisting of the amino acid sequence shown in SEQ ID NO: 2; or (b) a protein consisting of an amino acid sequence derived from the amino acid sequence shown in SEQ ID NO: 2 by substitution, deletion, or addition of one or more amino acid(s) and having the activity of inducing differentiation from monocytes into dendritic cell-like cells.

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Номер записи: 7
09-02-2012 дата публикации

Method for obtaining a singular cell model capable of reproducing in vitro the metabolic idiosyncrasy of humans

Номер: US20120034642A1
Автор: Agustín Lahoz Rodríguez, José Vicente Castell Ripoll, María José Gómez-Lechón, Ramiro Jover Atienza
Принадлежит: Advancell Advanced In Vitro Cell Technologies SA

The method is based on the use of expression vectors coding for the sense and anti-sense mRNA of the Phase I and Phase II drug biotransformation enzymes showing a greatest variability in humans for transforming cells expressing reductase activity. Such vectors can modulate (increase or decrease) the individualized expression of an enzyme without affecting the other enzymes. This singular cell model can reproduce in vitro the metabolic idiosyncrasy of humans. It is applicable in the study of development of new drugs, specifically in the study of metabolism, potential idiosyncratic hepatotoxicity, medicament interactions, etc., of new drugs.

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Номер записи: 8
09-02-2012 дата публикации

Prostate cancer cell lines and their use in screening method

Номер: US20120036587A1
Автор: Anne Chauchereau, Catherine Gaudin, Jean Benard, Karim Fizazi, Nader Al Nakouzi
Принадлежит: Institut Gustave Roussy (IGR)

The present invention relates to a prostate cancer cell line CNCM deposit number I-4126, the use thereof for preparing resistant prostate cancer cell lines, the resistant prostate cancer cell lines, and the use of these prostate cancer cell lines for screening compounds of interest.

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Номер записи: 9
16-02-2012 дата публикации

Multipotent Lymphohematopoietic Progenitor Cells

Номер: US20120040362A1
Автор: Igor I. Slukvin, James A. Thomson, Maksym A. Vodyanyk
Принадлежит: Individual

This invention relates to hematopoietic precursors derived from human embryonic stem cells. In the culture of differentiated cells from human ES cells, the fully committed hematopoietic precursors are CD34+ and CD43+ but not CD45+. If the cells are cultured until they express CD45, then the cells lose the ability to produce differentiated cells of the lymphoid lineages.

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Номер записи: 10
23-02-2012 дата публикации

Methods and uses of hypoxic compartment cells

Номер: US20120045419A1
Автор: Michael George Fehlings, Robert Davies Inman, William Mark Erwin
Принадлежит: UNIVERSITY HEALTH NETWORK

The disclosure relates to methods of maintaining and/or expanding an in vitro population of hypoxia compartment cells comprising culturing said population of cells optionally in an oxygen controlled environment, wherein the population of hypoxia compartment cells is exposed to an oxygen concentration of between about 1.5% and about 10%, preferably between about 2% and about 5%, and uses of cells expanded according to these methods.

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Номер записи: 11
01-03-2012 дата публикации

Differentiation of Pluripotent Stem Cells

Номер: US20120052576A1
Автор: Alireza Rezania
Принадлежит: Janssen Biotech Inc

The present invention provides methods to promote the differentiation of pluripotent stem cells into insulin producing cells. In particular, the present invention provides a method utilizing an agent that degrades retinoic acid to produce a population of pancreatic endocrine precursor cells.

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Номер записи: 12
01-03-2012 дата публикации

Peptide-modified microcarriers for cell culture

Номер: US20120052579A1
Автор: Florence Verrier, Simon Kelly Shannon
Принадлежит: Corning Inc

A cell culture article including a microcarrier having a peptide-modified polymer surface of the formula (I) where AAj represents at least one covalently bonded peptide, j is an integer of from 5 to 50, m, n, o, Sur, X, R, R′, and the mer ratio (m-o:n:o), including salts thereof, are as defined herein. Also disclosed are methods for making and using the cell culture article, as defined herein.

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Номер записи: 13
15-03-2012 дата публикации

Efficient Generation of Neurally-Induced Mesenchymal Stem Cells and Applications Thereof

Номер: US20120064041A1
Автор: Arshak Alexanian
Принадлежит: Individual

The present invention provides methods and compositions for reprogramming mammalian mesenchymal stem cells, as well as to methods for using such cells, for example, to prevent or treat various injuries, diseases, and disorders in human and non-human animals.

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Номер записи: 14
22-03-2012 дата публикации

Methods for producing nonadherent avian cell lines

Номер: US20120070893A9
Автор: Bertrand Pain, Fabienne Guehenneux
Принадлежит: Vivalis SA

The present invention relates to a method for producing avian cell lines, comprising gradual or complete withdrawal of growth factors, serum and/or feeder layer so that the established lines are adherent or nonadherent cells capable of proliferating indefinitely in a basic culture medium. The invention also relates to the cells derived from such lines which are particularly useful for the production of substances of interest.

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Номер записи: 15
22-03-2012 дата публикации

Method For Enucleating Nucleated Erythrocyte, And Enucleation Inducer

Номер: US20120070897A1
Автор: Eriko Shimamura, Hiroki Shimada, Toshihisa Hatta
Принадлежит: KANAZAWA MEDICAL UNIVERSITY

Provided is a factor capable of inducing enucleation, which is a final stage of erythrocyte differentiation, within a short time. More particularly, provided are a method of inducing enucleation, which is a final stage of erythrocyte differentiation, within a short time by adding a compound derived from proopiomelanocortin (POMC) to an undifferentiated (nucleated) erythrocyte, and an enucleation inducer including the compound.

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Номер записи: 16
29-03-2012 дата публикации

Animal-free cell culture method

Номер: US20120077268A1
Автор: Brigitte Ghislaine Louise Aerts, Carine Maggetto, Isabelle Solange Lucie Knott, Marie-Monique Jane Gonze, Yves Jules Maurice Ghislain
Принадлежит: GLAXOSMITHKLINE BIOLOGICALS SA

The present invention relates to a process for culturing animal cells, e.g., human, diploid anchorage-dependent cells, in the absence of exogenous components of primary animal origin. In particular, the invention provides cell culture media substantially free of exogenous components of primary and secondary animal origin which comprises at least one, more preferably several, exogenous animal-free growth factors. The present invention also relates to a process for cultivating animal cells using a protease of non-animal origin for passaging cells.

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Номер записи: 17
05-04-2012 дата публикации

In Vitro Generation of Myeloid Derived Suppressor Cells

Номер: US20120082688A1
Автор: Ping-Ying Pan, Shu-Hsia Chen, Zuping Zhou
Принадлежит: Mount Sinai School of Medicine

The invention relates to methods of isolating, culturing, and differentiating myeloid derived suppressor cells (MD-SCs) from embryonic stem (ES) cells and hematopoietic stem cells (HSCs). In certain embodiments, the invention relates to methods and compositions for producing MDSCs from ES cells and HSCs using a combination of factors including macrophage colony-stimulating factor (M-CSF).

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Номер записи: 18
12-04-2012 дата публикации

Immunotherapeutic agent

Номер: US20120087900A1
Автор: Tai-Gyu Kim
Принадлежит: Industry Academic Cooperation Foundation of Catholic University of Korea

The present invention is directed to adoptive immunotherapy using a lymphocyte in which an antigen-specific receptor and a bioactive material gene such as an IL-2 gene or a water-soluble TGF-beta receptor gene are transferred. The bioactive material is intensively secreted to, for example, a local site of a tumor, thereby reducing systemic side effects as much as possible, and the survival time of the lymphocyte is increased, thereby further improving the effect of the adoptive immunotherapy.

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Номер записи: 19
19-04-2012 дата публикации

Process for producing poxviruses and poxvirus compositions

Номер: US20120093780A1
Автор: Claude Sene, Daniel Malarme, Yves Cordier
Принадлежит: TRANSGENE SA

The present invention relates to compositions and pharmaceutical compositions comprising poxviruses and more particularly extracellular enveloped viruses. The present invention also relates to a process for producing poxviruses and poxviruses obtained thereof. Moreover, the present invention also relates to the use of said poxvirus and said composition for the preparation of a medicament.

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Номер записи: 20
19-04-2012 дата публикации

Treatment of limb ischemia

Номер: US20120093786A1
Автор: Erik Miljan, John Sinden, Paolo Madeddu
Принадлежит: ReNeuron Ltd

The present invention uses neural stem cells in the manufacture of a medicament for the treatment of a patient suffering peripheral arterial disease. The invention is particularly suited for treating limb ischemia or Buerger's disease.

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Номер записи: 21
19-04-2012 дата публикации

Methods of neural conversion of human embryonic stem cells

Номер: US20120094381A1
Автор: Lorenz Studer, Stuart Chambers
Принадлежит: Memorial Sloan Kettering Cancer Center

The present invention relates generally to the field of cell biology of stem cells, more specifically the directed differentiation of pluripotent or multipotent stem cells, including human embryonic stem cells (hESC), somatic stem cells, and induced human pluripotent stem cells (hiPSC) using novel culture conditions. Specifically, methods are provided for obtaining neural tissue, floor plate cells, and placode including induction of neural plate development in hESCs for obtaining midbrain dopamine (DA) neurons, motorneurons, and sensory neurons. Further, neural plate tissue obtained using methods of the present inventions are contemplated for use in co-cultures with other tissues as inducers for shifting differentiation pathways, i.e. patterning.

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Номер записи: 22
26-04-2012 дата публикации

Regionalised endoderm cells and uses thereof

Номер: US20120100115A1
Автор: Gillian Mary Morrison, Ifigenia Oikonomopoulou, Joshua Mark Brickman
Принадлежит: University of Edinburgh

The present invention relates to the generation of anterior definitive endoderm (ADE) cells from embryonic stem cells and the differentiation of such cells to, for example, pancreatic or liver cells. The invention also relates to cell lines, cell culture methods, cells markers and the like and their potential uses in a variety of applications.

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Номер записи: 23
03-05-2012 дата публикации

Compositions and methods for modifying cell surface glycans

Номер: US20120107281A1
Автор: Robert Sackstein
Принадлежит: Individual

Methods and compositions for modifying glycans (e.g., glycans expressed on the surface of live cells or cell particles) are provided herein.

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Номер записи: 24
03-05-2012 дата публикации

Human Late Stage Motor Neuron Progenitor Cells and Methods of Making and Using Same

Номер: US20120107934A1
Автор: Aleksandra Poole
Принадлежит: California Stem Cells Inc

Motor neuron progenitor (MNP) cells and populations of MNP cells, are provided, in particular, populations of human late stage MNP cells having a purity of greater than about 65% late stage MNP cells and high-purity populations of MNP cells having greater than 95% viable cells, as well as method of making and using the same, including deriving late stage MNP cells from pluripotent embryonic stem cells, producing high-purity populations of late stage MNP cells, producing populations of viable MNP cells, transporting viable MNP cells, and transplanting MNP cells.

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Номер записи: 25
10-05-2012 дата публикации

Cd4+cd25- t cells and tr1-like regulatory t cells

Номер: US20120114597A1
Автор: Detlef Dieckmann, Gerold Schuler
Принадлежит: Merix Bioscience Inc

The invention provides CD4 + CD25 − T cells and Tr1-like regulatory T cells (i.e., contact-independent Type 1-like regulatory T cells), processes for their production and their use for regulatory purposes.

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Номер записи: 26
10-05-2012 дата публикации

Dendritic Cell Compositions and Methods

Номер: US20120114680A1
Автор: Irina Tcherepanova, Lois Dinterman, Rebecca Pogue-Caley, Tamara Monesmith
Принадлежит: Merix Bioscience Inc

Methods are provided for the production of dendritic cells from monocytes that have been incubated at a temperature of 1° C.-34° C. for a period of approximately 6 to 96 hours from the time they are isolated from a subject. After the incubation period, the monocytes can then be induced to differentiate into dendritic cells. Mature dendritic cells made by the methods of the invention have increased levels of one or more of CD80, CD83, CD86, MHC class I molecules, or MHC class II molecules as compared to mature dendritic cells prepared from monocytes that have not been held at 1° C.-34° C. for at least 6 hours from the time they were isolated from a subject. Dendritic cells made by the methods of the invention are useful for the preparation of vaccines and for the stimulation of T cells.

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Номер записи: 27
10-05-2012 дата публикации

Smac mimetec

Номер: US20120115922A1
Автор: Matthew G. Laporte, Stephen M. Condon, Susan R. Rippin, Yijun Deng
Принадлежит: TETRALOGIC PHARMACEUTICALS CORP

A SMAC mimetic and pharmaceutical compositions thereof and methods of use.

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Номер записи: 28
17-05-2012 дата публикации

Anti-estrogen and immune modulator combinations for treating breast cancer

Номер: US20120121620A1
Автор: David A. Sirbasku
Принадлежит: Individual

Compositions for treating cancers of mucosal tissues including breast, prostate, ovary, colon are disclosed which include various combinations of new or conventional anti-estrogen compounds, aromatase inhibitors, immune modulators, immune inhibitors, immune inhibitor mimicking compounds and steroid or thyroid hormones. Methods of predicting susceptibility of a cancer of mucosal origin to treatment with a composition containing an immune inhibitor or an immune inhibitor mimicking compound are also disclosed. Preferred methods include identifying in a specimen of cancer cells the presence of a Poly-Ig (Fe) receptor or Poly-Ig-like (Fc) receptor capable of binding to an immune inhibitor or an immune inhibitor mimicking compound and of mediating immune inhibition of cancer cell growth.

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Номер записи: 29
17-05-2012 дата публикации

Recombinant protein of fibroblast growth factor having adhesive activity for stem cells and method for culturing stem cells using the same

Номер: US20120122156A1
Автор: Min Han, Sang-Heon Kim, Soo Hyun Kim, Young Mee Jung
Принадлежит: Korea Advanced Institute of Science and Technology KAIST

The present invention relates to a recombinant protein of a fibroblast growth factor (FGF) having an adhesive activity for stem cells and a method for culturing stem cells using the same. More particularly, the present invention relates to a recombinant protein having an adhesive activity for stem cells by fusion of a polypeptide linker at amino terminal of FGF, and a method for culturing stem cells using immobilized FGF comprising: fixing the recombinant protein in a culture vessel with a hydrophobic surface using amino terminal of the polypeptide linker, adhering stem cells on the recombinant protein-fixed culture vessel, and culturing the stem cells.

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Номер записи: 30
17-05-2012 дата публикации

Nanotopographic compositions and methods for cellular organization in tissue engineered structures

Номер: US20120122222A1
Автор: David Carter, Jeffrey T. Borenstein, Joseph P. Vacanti
Принадлежит: Charles Stark Draper Laboratory Inc, General Hospital Corp

The present invention relates to tissue engineered compositions and methods comprising nanotopographic surface topography (“nanotopography”) for use in modulating the organization and/or function of multiple cell types.

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Номер записи: 31
24-05-2012 дата публикации

Induced pluripotent stem cells

Номер: US20120128655A1
Автор: Chun-Hyung Kim, Dohoon Kim, Kwang-Soo Kim
Принадлежит: Mclean Hospital Corp

The present invention concerns the delivery of certain reprogramming factor proteins into cells, such as differenti-atedsomatic cells, in order to induce the epi-genetic reprogramming of the cell so it becomes a pluripotent stem cell. The reprogramming factor protein(s) may be Sox2, Klf4, Oct3/4, c-Myc, Lin28, Nanog, or any protein with reprogramming (-enhancing) activity. These proteins may be linked recombinantly or chemically to a cell penetrating peptide that helps facilitate the introduction of these proteins into the target cell and may be preferably expressed in mammalian cells to maintain them in active forms. Accordingly, the present method of inducing pluripotent stem cell (iPS) formation avoids the use of viral or DNA-based expression vectors or the expression of reprogramming factor genes within target cells, which are known to be harmful to the host target cell and cause cancer.

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Номер записи: 32
24-05-2012 дата публикации

Compositions and methods of functionally enhanced in vitro cell culture system

Номер: US20120129207A1
Автор: Erik Novik, Martin Yarmush, Robert Freedman, YAAKOV Nahmias
Принадлежит: General Hospital Corp, Hurel Corp

Compositions and methods described herein provide a cell culture system in which cells are in high metabolic states from the onset of the culture. Combinations of various cell culture components disclosed and employed herein allow cells to be in high metabolic states useful for drug testing immediately after the start of cell culture.

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Номер записи: 33
24-05-2012 дата публикации

Method of preparing pluripotent stem cells

Номер: US20120129256A1
Автор: Mariya A. Lagar'kova, Mariya V. Shutova, Sergey L. Kiselev
Принадлежит: OBSCHESTVO S OGRANICHENNOI OTVETSTVENNOSTYU "LABORATORIA KLETOCHNYKH TEKHNOLOGIY\

The invention relates to biotechnology, and particularly to the preparation of pluripotent stem cells. The method involves introduction into umbilical cord and placental stem cells of RNA with at least one sequence which ensures the transition of cells to the pluripotent state. The method enables to effectively prepare pluripotent stem cells from the cells of mammalian placenta and umbilical cord which have not yet acquired somatic mutations, which reduces the risk of oncogenesis and other adverse effects of reprogramming.

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Номер записи: 34
24-05-2012 дата публикации

Transcriptome Transfer Produces Cellular Phenotype Conversion

Номер: US20120129261A1
Автор: Jai-Yoon Sul, James Eberwine, Tai Kyung Kim, Vickas Patel
Принадлежит: University of Pennsylvania Penn

The present invention includes methods for effecting phenotype conversion in a cell by transfecting the cell with phenotype-converting nucleic acid. Expression of the nucleic acids results in a phenotype conversion in the transfected cell. Preferably the phenotype-converting nucleic acid is a transcriptome, and more preferably an mRNA transcriptome.

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Номер записи: 35
07-06-2012 дата публикации

Use of catalytic antioxidant to preserve stem cell phenotype and control cell differentiation

Номер: US20120141432A1
Автор: Bridgette M. Deasy, Jon D. Piganelli, Steven M. Chirieleison
Принадлежит: University of Pittsburgh

Methods are disclosed herein for maintaining stem cells in an undifferentiated state in vitro. The methods include contacting the stem cells with an effective amount of a catalytic antioxidant. Also disclosed are methods for the increasing the number of stem cells in vitro while maintaining the stem cells in an undifferentiated state. The methods include contacting the stem cells with an effective amount of a catalytic antioxidant and an effective amount of one or more growth factors that promotes the expansion of the stem cells.

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Номер записи: 36
07-06-2012 дата публикации

Compositions and methods for promoting beta cell maturity

Номер: US20120141436A1
Автор: Arun Sharma, Cristina Aguayo-Mazzucato, Susan Bonner-Weir
Принадлежит: Joslin Diabetes Center Inc

Compositions and methods for providing an enriched population of mature, glucose-responsive insulin secreting cells, and for modulating insulin expression, activity and secretion in a subject.

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Номер записи: 37
07-06-2012 дата публикации

Methods and Compositions for Treatment of Muscular Dystrophy

Номер: US20120141441A1
Автор: Alfonso P. FARRUGGIO, Christopher Bjornson, Christopher L. Chavez, Chunli Zhao, Hassan Chaib, Jonathan M. Geisinger, Marisa Karow, Michele P. Calos, Tawny Neal
Принадлежит: Leland Stanford Junior University

The present disclosure provides methods for introducing a gene encoding a muscle membrane protein into a cell isolated from a subject to generate a genetically modified cell. The genetically modified cell may be introduced back, e.g., engrafted into the subject. The isolated cell may be additionally modified by introducing into the isolated cell a gene encoding one or more reprogramming transcription factors that induce the cell to form an induced pluripotent stem cell. The genetically modified cell may be differentiated in vitro to form muscle cell precursors before engrafting into the subject. Also provided are compositions comprising autologous cells isolated from a subject which cells comprise a muscle membrane protein gene integrated into a genome attachment site in the genome of the cell. The autologous cell may be an induced pluripotent cell or a mesenchymal stem cell, such as an adipose-derived mesenchymal stem cell (AD-MSC).

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Номер записи: 38
07-06-2012 дата публикации

Cell coated implantable device

Номер: US20120141562A1
Автор: George A. Truskey, Hardean E. Achneck, Ryan M. Jamiolkowski
Принадлежит: Achneck Hardean E, Jamiolkowski Ryan M, Truskey George A

The present invention relates, in general, to a cell-coated implantable medical device and, in particular, to an implantable medical device the blood-contacting surfaces of which are coated with endothelial progenitor cells (EPCs). In a preferred embodiment, the medical device is a titanium or titanium alloy-based medical device.

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Номер записи: 39
07-06-2012 дата публикации

Method for inducing differentiation into epithelial progenitor cell/stem cell population and corneal epithelial cell population from induced pluripotent stem cells

Номер: US20120142103A1
Автор: Keisuke Okita, Kohji Nishida, Miharu Sakurai, Ryuhei Hayashi, Shinya Yamanaka, Tomofumi Kageyama
Принадлежит: KYOTO UNIVERSITY, Tohoku University NUC

The present invention relates to: a method for inducing differentiation into an epithelial progenitor cell/stem cell population or a corneal epithelial cell population by culturing, under particular conditions, induced pluripotent stem cells induced from mammalian somatic cells or undifferentiated stem cells; an epithelial progenitor cell/stem cell population or a corneal epithelial cell population obtained by the method; and a cell preparation for the treatment of epithelial disease and a cell sheet, which are prepared using these cell populations.

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Номер записи: 40
14-06-2012 дата публикации

Composition for hard tissue formation and, dentin or pulp regeneration containing ameloblast, apical bud cell or its culture fluid as an active ingredient

Номер: US20120148538A1
Автор: Jihyun Lee, Joo-Cheol PARK, Won-Jun SHON
Принадлежит: SNU R&DB FOUNDATION

The present invention relates to a composition for hard tissue formation and dentin, or dental pulp regeneration comprising ameloblasts, apical bud cells, or its culture fluid as an active ingredient. The ameloblasts, apical bud cells, or its culture fluid promotes the differentiation of odontoblasts, human dental pulp stem cells and osteoblasts, increases DSPP promoter activity, induces dental pulp regeneration of dental pulp stem cells, and induces hard tissue formation significantly in vivo, so that they can be effectively used for a composition for promoting hard tissue formation and dentin or dental pulp regeneration.

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Номер записи: 41
14-06-2012 дата публикации

Methods and Compositions for Tissue Engineering

Номер: US20120148539A1
Автор: Moulay Hicham Alaoui-Ismaili
Принадлежит: Individual

Disclosed herein are methods and compositions for promoting endochondral bone formation. The invention includes methods of promoting endochondral bone formation by down-regulating the expression of the DIO2 gene or the activity of the deiodinase protein. The invention also includes methods of up-regulating the activity of the FGFR3, ADAMTS9, HEY1, HAS3, and/or MFI2 genes and/or the activity of the expression products of those genes to promote endochondral bone formation. The invention also includes compositions of BMP-7 and agonists of FGFR3, ADAMTS9, HEY1, HAS3, and/or MFI2 proteins. Compositions can further include mesenchymal stem cells.

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Номер записи: 42
14-06-2012 дата публикации

Synthetic graft

Номер: US20120148543A1
Автор: Che Connon
Принадлежит: Individual

The present invention relates to the use of a plastically-compacted collagen gel as a substrate for the growth of corneal cells, particularly limbal corneal epithelial stem cells. Cells grown on such a substrate can be cultured to produce artificial ocular epithelia which can be used in ocular toxicity testing or for transplantation.

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Номер записи: 43
14-06-2012 дата публикации

Methods for complex tissue engineering

Номер: US20120148632A1
Автор: Barbara Pui Chan, Daniel Tsz Kit Chik, Hiu Wa Cheng, Keith Dip Kei Luk, Kenneth Man Chee Cheung
Принадлежит: University of Hong Kong HKU

A simple, highly flexible and scalable platform for making functional complex tissues with heterogeneity and irregularity is provided. The method includes combining undifferentiated cells, such as pluripotent or multipotent stem cells, with a biomaterial to make multiple undifferentiated or naïve subunits, exposing the undifferentiated or naïve subunits to different cell culture environments for induction of differentiation towards different lineages as required by that complex tissue, and combining the then functional subunits with or without the undifferentiated subunits. The differentiated subunits thus combined can be cultured under biological, chemical, and/or physical culture conditions suitable to fine-tune the structural and functional properties of the bioengineered complex tissue to form a bioengineered tissue graft that mimics the structural and functional characteristics of native complex tissue. The bioengineered tissue graft can then used to replace dysfunctional tissue.

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Номер записи: 44
14-06-2012 дата публикации

Method and System for Monitoring Nutritional Uptake as a Function of Microflora Intestinal Gas Levels

Номер: US20120150153A1
Автор: Cholwon Koh, Inyoung Sa, John Gavin MacDonald, Jose K. Abraham, Sridhar Ranganathan
Принадлежит: Kimberly Clark Worldwide Inc

Systems and methods for monitoring nutritional uptake of an individual are disclosed. The method can include monitoring microflora intestinal gas concentration levels associated with a patient and adjusting the volume of nutrient provided by the patient with an artificial feeding device based at least in part on the microflora intestinal gas levels associated with the patient. A microflora intestinal gas sensor can be used to monitor the microflora intestinal gas associated with the patient. The microflora intestinal gas sensor can monitor the microflora intestinal gas in a patient's exhaled breath or in the patient's digestive tract. The microflora intestinal gas sensor be included as part of an enteral feeding system at the distal end or outside end of an enteral feeding tube. Systems and methods for monitoring nutritional uptake of an infant based on microflora intestinal gas levels associated with the infant are also disclosed.

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Номер записи: 45
21-06-2012 дата публикации

Method for inducing extended self-renewal of functionally differentiated somatic cells

Номер: US20120156179A1
Автор: Michael Sieweke
Принадлежит: CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS, Institut National de la Sante et de la Recherche Medicale INSERM, Universite de la Mediterranee Aix Marseille II

The present invention relates to a method for inducing proliferation of functionally differentiated somatic cells comprising a step of activating expression of a Myc family gene and a KIf family gene in said cells or contacting said cells with a Myc family protein and a KIf family protein.

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Номер записи: 46
28-06-2012 дата публикации

Cyclic di-amp induction of type i interferon

Номер: US20120164107A1
Автор: Daniel A. Portnoy, Joshua J. Woodward
Принадлежит: UNIVERSITY OF CALIFORNIA

Methods of modulating type-I interferon production in a cell are provided. Aspects of the methods include modulating cytosolic cyclic di-adenosine monophosphate (c-di-AMP) activity in the cell in a manner sufficient to modulate type-I interferon production in the cell. Additional aspects of the invention include c-di-AMP activity modulatory compositions, e.g., c-di-AMP, mutant Listeria bacteria, cyclase and/or phosphodiesterase nucleic acid or protein compositions, etc. The subject methods and compositions find use in a variety of applications, including therapeutic applications.

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Номер записи: 47
05-07-2012 дата публикации

Spontaneously contracting fish cell aggregates, use thereof and method for the production thereof

Номер: US20120171695A1
Автор: Bianka Grunow, Charli Kruse, Daniel Hans Rapoport, Philipp Ciba
Принадлежит: Fraunhofer Gesellschaft zur Forderung der Angewandten Forschung eV

The invention relates to an in vitro method for producing spontaneously contracting fish cell aggregates, to the fish cell aggregates obtained thereby, and to the use thereof, in particular for testing biological active substances and pharmaceuticals. The in vitro method according to the invention for producing contracting fish cell aggregates comprises the following steps: a) mechanical comminution and/or partial enzymatic digestion of fish embryos or fish larvae; b) transfer of the comminuted and/or partially digested fish tissue into an enzyme-free medium and removing the supernatant by centrifuging to obtain a cell pellet; c) re-suspension of the cell pellet in a cell culture medium; d) culturing the cells, with the medium being changed at least once, until spontaneously contracting cell aggregates form.

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Номер записи: 48
19-07-2012 дата публикации

Methods and Compositions For Reprogramming Cells

Номер: US20120184035A1
Автор: Katerine L. Holton, Robert Lanza, Sadhana Agarwal
Принадлежит: Individual

Methods and compositions are provided for reprogramming cells. In an exemplary embodiment, fibroblasts are reprogrammed to adopt a skeletal, cardiac, or smooth muscle cell fate. Cell and tissue therapies using said methods and compositions are also disclosed.

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Номер записи: 49
26-07-2012 дата публикации

Induced pluripotent stem cells and methods of use

Номер: US20120189595A1
Автор: Angel Raya, Juan Carlos Izpisua Belmonte, Trond Aasen
Принадлежит: SALK INSTITUTE FOR BIOLOGICAL STUDIES

The invention relates to the field of stem cells and, specially, to the reprogramming of adult somatic cells; to obtain pluripotent cells by the transfection of specific genes. Thus, the invention provides induced pluripotent stem cells (iPS) and methods of obtaining and using them.

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Номер записи: 50
26-07-2012 дата публикации

Method of producing a medical composition

Номер: US20120189663A1
Автор: Hiroshi Takaku, Kazuo Wakabayashi, Myint Oo Chang, Norio Yamamoto, Tomoyuki Suzuki
Принадлежит: Chiba Institute of Technology

The present invention aims to produce a safe and reliable medical composition, which efficiently boosts nonspecific immunity of antigen-presenting cells and thereby promotes an antitumor activity. To produce the medical composition, antigen-presenting cells such as dendritic cells are exposed to an activating reagent containing baculoviruses. Then, the antigen-presenting cells are separated from the activating reagent. The antigen-presenting cells are optionally cultured after the separation. Furthermore, an absence of the baculoviruses in the composition are optionally checked. The medical composition produced by the present invention is expected to have an outstanding therapeutic effect.

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Номер записи: 51
26-07-2012 дата публикации

Methods for obtaining hepatocytes, hepatic endoderm cells and hepatic progenitor cells by induced differentiation

Номер: US20120190059A1
Автор: Dongxin Zhao, Hongkui Deng, Mingxiao Ding, SONG Chen, Zhihua Song
Принадлежит: Beijing Huayuanbochuang Technology Co Ltd

The present invention discloses a method for inducing the differentiation of embryonic stem cells (ESC) or induced pluripotent stem cells (iPS cells) into hepatocytes, a method for inducing the differentiation of embryonic stem cells or induced pluripotent stem cells into hepatic endoderm cells, and a method for inducing the differentiation of embryonic stem cells (ESC) or induced pluripotent stem cells into hepatic progenitor cells. The present invention also provides the hepatocytes, hepatic endoderm cells and hepatic progenitor cells obtained by above methods, and the uses of these cells.

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Номер записи: 52
26-07-2012 дата публикации

Culture Medium and Methods for Producing Alginate From Stable Mucoid Strains of Pseudomonas Aeruginosa

Номер: US20120190077A1
Автор: Hongwei David Yu, Kristy Dawn Dillon, Richard M. NILES, XIN Wang
Принадлежит: PROGENESIS Tech LLC

A specialized culture medium for the promotion of alginate production by stable mucoid Pseudomonas aeruginosa bacterial strains and methods for the production and purification of industrial, commercial, and pharmaceutical grade alginate from bacteriological sources are provided herein. Alginate produced using the media and methods disclosed herein is structurally uniform and substantially free of bacterial cell contaminants, including endotoxin.

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Номер записи: 53
26-07-2012 дата публикации

Differentiation of Pluripotent Stem Cells

Номер: US20120190111A1
Автор: Christine Parmenter, Janet Davis, Jiajian Liu, Pascal Ghislain André Bonnet
Принадлежит: Janssen Biotech Inc

The present invention is directed to methods to differentiate pluripotent stem cells. In particular, the present invention is directed to methods and compositions to differentiate pluripotent stem cells into cells expressing markers characteristic of the definitive endoderm lineage comprising culturing the pluripotent stem cells in medium comprising a sufficient amount of GDF-8 to cause the differentiation of the pluripotent stem cells into cells expressing markers characteristic of the definitive endoderm lineage.

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Номер записи: 54
26-07-2012 дата публикации

Macroporous Microcarrier Specific to Liver Cell, Preparation Method and Use Thereof

Номер: US20120190113A1
Автор: Duhui Gong, Huancheng Zhou, Mingxin Pan, YI Gao, Zhi Zhang, Zhiwei Hu
Принадлежит: Individual

The present invention provides a macroporous microcarrier specific to hepatocytes using silk fibroin and galactosylated chitosan as main raw material, a preparation method thereof, and application for hepatocyte culture under the culture condition of microgravity rotation. The macroporous microcarrier s a sphere prepared from silk fibroin and galactosylated chitosan under the effect of crosslinker, wherein based on the total weight of the sphere, the content of silk fibroin is 50-80 wt % and the content of galactosylated chitosan is 15-40 wt %. The diameter of the microcarrier is 200-500 μm, and the aperture of the microcarrier is 40-80 μm. Compared with normal solid scaffold material, the microcarrier provided by the present invention has larger surface area/volume ratio and, a sinus gap structure extremely similar with in-vivo liver sinus structure, therefore it is more conducive to adhering of the hepatocytes on the scaffold material, contacting between cells, transporting oxygen and nutrient components and excreting metabolic products.

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Номер записи: 55
02-08-2012 дата публикации

Treatment of acne by conditioned media

Номер: US20120195969A1
Автор: Neil H. Riordan, Tierney M. Riordan
Принадлежит: AIDAN RES AND CONSULTING LLC

Disclosed are methods and compositions for treatment of acne or acneform conditions, particularly but not limited to, acne vulgaris with products generated from culture of stem or progenitor cells. Specifically, compositions of matter are disclosed which are useful for the treatment of acne and acne associated disease states, in particular acne vulgaris, by topical administration of products derived from stem cells or progenitor cells.

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Номер записи: 56
02-08-2012 дата публикации

Sperm Diluent Solution and Method for Artificial Insemination Using Same

Номер: US20120197068A1
Автор: Masayuki Shimada, Tetsuji Okazaki
Принадлежит: Individual

A sperm diluent of the present invention contains a chelating agent such as EDTA and/or, EGTA, which forms a complex with a calcium ion, in a base diluent. Further, the sperm diluent contains an immunosuppressive factor such as a steroid hormone and/or, a cytokine, which suppresses migration of leukocytes. By diluting frozen sperm with this sperm diluent followed by performing artificial insemination, death of the sperm before fertilization and phagocytosis of the sperm and embryos by leukocytes in the uterus can be suppressed, allowing enhancement of the conception rate and the implantation rate.

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Номер записи: 57
16-08-2012 дата публикации

Adipose-derived stem cells and lattices

Номер: US20120208274A1
Автор: Adam J. Katz, Hermann Peter Lorenz, J. William Futrell, Marc H. Hedrick, Min Zhu, Patricia Zuk, Peter H. Ashjian, Prosper Benhaim, Ramon Llull
Принадлежит: Individual

The present invention provides adipose-derived stem cells (ADSCs), adipose-derived stem cell-enriched fractions (ADSC-EF) and adipose-derived lattices, alone and combined with the ADSCs of the invention. In one aspect, the present invention provides an ADSC substantially free of adipocytes and red blood cells and clonal populations of connective tissue stem cells. The ADSCs can be employed, alone or within biologically-compatible compositions, to generate differentiated tissues and structures, both in vivo and in vitro. Additionally, the ADSCs can be expanded and cultured to produce molecules such as hormones, and to provide conditioned culture media for supporting the growth and expansion of other cell populations. In another aspect, the present invention provides a adipose-derived lattice substantially devoid of cells, which includes extracellular matrix material from adipose tissue. The lattice can be used as a substrate to facilitate the growth and differentiation of cells, whether in vivo or in vitro, into anlagen or even mature tissues or structures.

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Номер записи: 58
23-08-2012 дата публикации

Immunoneutral silk-fiber-based medical devices

Номер: US20120210547A1
Автор: David J. Horan, Gregory H. Altman, Jingsong Chen, Rebecca Horan
Принадлежит: Allergan Inc

Silk is purified to eliminate immunogenic components (particularly sericin) and is used to form fabric that is used to form tissue-supporting prosthetic devices for implantation. The fabrics can carry functional groups, drugs, and other biological reagents. Applications include hernia repair, tissue wall reconstruction, and organ support, such as bladder slings. The silk fibers are arranged in parallel and, optionally, intertwined (e.g., twisted) to form a construct; sericin may be extracted at any point during the formation of the fabric, leaving a construct of silk fibroin fibers having excellent tensile strength and other mechanical properties.

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Номер записи: 59
23-08-2012 дата публикации

Cardiomyocytes and methods of producing and purifying cardiomyocytes

Номер: US20120213748A1
Автор: Gabriel Nistor
Принадлежит: Individual

The invention provides methods for producing a culture of cardiomyocytes and cultures of cardiomyocytes. Exemplary methods of producing and cultures of cardiomyocytes include a population of cells including cells having spontaneous and periodic electrical activity, and/or including nodal, sino-atrial or pacemaker cells; immature cardiomyocytes (cardiomyoblasts); mature contractile cardiomyocytes; or a mixed population of two or more of such cells.

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Номер записи: 60
23-08-2012 дата публикации

Method of Preconditioning of Cell Suspensions

Номер: US20120213754A1
Автор: John R. Chapman
Принадлежит: STEM CELL PARTNERS LLC

An apparatus for the preparation of cells for implantation into a living body is disclosed. The apparatus comprises a vessel substantially impermeable to gaseous oxygen; and fluid within said vessel, the fluid having a maximal dissolved oxygen capacity substantially equivalent to normal saline yet having a hypoxic oxygen concentration between about 0% to about 5% of said maximal dissolved oxygen capacity. The bag oxygen concentration level remains low when the apparatus is stored at 22°-25° C. in normal atmospheric conditions for a period of at least 30 days. The present invention simplifies the process of achieving donor cell hypoxic preconditioning for cell implantation, and may be used to bathe said cells to be transplanted for a sufficient time to activate the hypoxic metabolic pathway.

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Номер записи: 61
23-08-2012 дата публикации

Process for demineralization of bone matrix with preservation of natural growth factors

Номер: US20120213859A1
Автор: Benjamin P. Luchsinger, Darrel L. Holmes, Gregory A. Juda, Jesus Hernandez, Kelly R. Kirker, Nancy J. Shelby, Steven M. Scott
Принадлежит: Bacterin International Inc

A demineralized bone matrix is produced by a process in which a bone body is placed in a first processing solution comprising an acid to demineralize the bone body. The bone body is periodically removed from the first solution at specific time intervals to perform at least one test, such as a compression test, on a mechanical property of the bone body. When the test yields a desired result, the bone body is exposed to a second processing solution that is less acidic than the first, thus minimizing the exposure of the bone body to the harsh acidic conditions of the demineralization phase of the process.

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Номер записи: 62
23-08-2012 дата публикации

Generating induced pluripotent stem cells and progenitor cells from fibroblasts

Номер: US20120214236A1
Автор: Éva SZABÓ, Kausalia Vijayaragavan, Mickie Bhatia, Ruth Munoz Risueno, Shravanti Rampalli-Deshpande
Принадлежит: MCMASTER UNIVERSITY

The present disclosure provides a method of generating progenitor cells, such as hematopoietic or neural progenitor cells, from fibroblasts, such as dermal fibroblasts, comprising providing fibroblasts that express or are treated with a POU domain containing gene or protein and culturing the cells under conditions that allow production of progenitor cells, without traversing the pluripotent state. Also provided is a method of isolating a subpopulation of fibroblasts with reprogramming potential comprising providing fibroblasts that express an Oct-4-reporter and isolating cells that are positive for the reporter. Further provided is a method of generating reprogrammed fibroblast-derived induced pluripotent stem cells. Also provided are uses and assays of the cells produced by the methods of the disclosure.

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Номер записи: 63
30-08-2012 дата публикации

Freeze-dried fibrin matrices and methods for preparation thereof

Номер: US20120219534A1
Автор: Avner Yayon, Malkit Azachi, Micha Gladnikoff
Принадлежит: Prochon Biotech Ltd

Methods for treating diseased or injured tissue by implanting into the tissue at a site of the disease or injury a porous freeze-dried fibrin matrix formed from plasma proteins. The proteins include fibrinogen cleaved by the action of thrombin at varying concentrations sufficient to cleave the fibrinogen and Factor XIII. The matrix has less than 10% residual moisture and is devoid of exogenous anti-fibrinolytic agents, plasminogen and of organic chelating agents. Alternatively, the plasma proteins comprise partially purified plasma proteins that are devoid of plasminogen.

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Номер записи: 64
30-08-2012 дата публикации

Optimizing culture medium for cd34<+> hematopoietic cell expansion

Номер: US20120220032A1
Автор: Chao-Ling Yao, Chi-Hsien Liu, I-Ming Chu, Shiaw-Min Hwang, Tzu-Bou Hsieh
Принадлежит: SINO CELL Tech Inc

The present invention provides a method of determining the optimal composition of a serum-free, eukaryotic cell culture medium supplement, using 2-level factorial design and the steepest ascent method. The invention further provides a method of making a serum-free eukaryotic cell culture medium supplement and the generated thereof. The invention further provides a method of making a serum-free, eukaryotic cell culture medium and the medium generated thereof. The invention further provides a kit containing the medium of the invention. The invention also provides a method of expanding CD34<+> hematopoietic cells and a composition comprising CD34<+> hematopoietic cells in a serum-free, eukaryotic cell culture medium of the invention.

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Номер записи: 65
30-08-2012 дата публикации

Immunologically modified carbon nanotubes for cancer treatment

Номер: US20120220921A1
Автор: Wei R. Chen
Принадлежит: University of Central Oklahoma

A method for constructing a compound of immunologically modified nanotubes and method for using the compound to deliver immunoadjuvants to tumor cells and to produce targeted, synergistic photophysical and immunological reactions for cancer treatment. To prepare the immunologically modified nanotubes, carbon nanotubes are dissolved in a solution of glycated chitosan, an immunostimulant, hence using glycated chitosan as a surfactant for rendering the aqueous solution of nanotubes stable. The compound can be used for treatment of cancer. The method includes steps of intratumorally administering immunologically modified nanotubes and administering laser irradiation of the target tumor. The nanotube serves as a carrier to deliver immunoadjuvants to the tumor cells and serves as a light-absorbing agent in a cell body of a tumor in a host. Upon laser irradiation of target tumor cells, immunologically modified nanotubes inside the tumor cells can produce spatially and temporally synchronized photothermal and immunological reactions for cancer treatment.

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Номер записи: 66
13-09-2012 дата публикации

Conditioned cell culture medium compositions and methods of use

Номер: US20120230940A1
Автор: Andreas Kern, Anthony Ratcliffe, David L. Horwitz, Gail K. Naughton, Joan Zeltinger, Jonathan N. Mansbridge, Lee K. Landeen, Mark A. Applegate, R. Emmett Pinney
Принадлежит: Skinmedica Inc

Novel products comprising conditioned cell culture medium compositions and methods of use are described. The conditioned cell medium compositions of the invention may be comprised of any known defined or undefined medium and may be conditioned using any eukaryotic cell type. Once the cell medium of the invention is conditioned, it may be used in any state. Physical embodiments of the conditioned medium include, but are not limited to, liquid or solid, frozen, lyophilized or dried into a powder. Additionally, the medium is formulated with a pharmaceutically acceptable carrier as a vehicle for internal administration, applied directly to a food item or product, or formulated with a salve or ointment for topical applications. Also, the medium may be further processed to concentrate or reduce one or more factors or components contained within the medium.

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Номер записи: 67
13-09-2012 дата публикации

Tissue transplant compositions and methods for use

Номер: US20120230966A1
Автор: John M. Garvey, Keith D. Crawford, Pamela Layton
Принадлежит: Brigham and Womens Hospital Inc, PARCELL LABS LLC

Provided are transplants and methods for augmenting formation and restoration of organ and tissue, for example, bone formation, by administering autologous or allogeneic human embryonic-like adult stem cells (ELA cells). Also provided is a method for augmenting formation of tissues and organs by administering a transplant having ELA stem cells or combination of ELA stem cells.

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Номер записи: 68
13-09-2012 дата публикации

Production of beta-cells

Номер: US20120231454A1
Автор: Ahmed Mansouri, Patrick Collombat
Принадлежит: Max Planck Gesellschaft zur Foerderung der Wissenschaften eV

The present invention relates to in vitro and in vivo methods for the generation of pancreatic β-cells, comprising the step of providing at least one pancreatic α-cell or at least one precursor cell with Pax-4 or a nucleic acid encoding Pax-4. Furthermore, the invention relates to a screening method for the screening of a pancreatic-β-cell phenotype-inducing compound, comprising the step of contacting at least one pancreatic α-cell or at least one precursor cell with a given compound, and testing whether said compound is capable of inducing a pancreatic-β-cell phenotype.

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Номер записи: 69
20-09-2012 дата публикации

Functionalizing implantable devices with a poly (diol citrate) polymer

Номер: US20120237443A1
Автор: Guillermo Ameer, Jian Yang
Принадлежит: Northwestern University

The present invention is directed to a novel poly (diol citrates)-based coating for implantable devices. More specifically, the specification describes methods and compositions for making and using implantable devices coated with citric acid copolymers or citric acid copolymers impregnated with therapeutic compositions and/or cells.

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Номер записи: 70
20-09-2012 дата публикации

Compositions And Methods For Cell Based Retinal Therapies

Номер: US20120237473A1
Автор: Anton Kolomeyer, Ilene Sugino, Marco Zarbin
Принадлежит: UNIVERSITY OF MEDICINE AND DENTISTRY OF NEW JERSEY

The invention relates to pharmaceutical compositions comprising trophic factors, methods to decrease the degeneration of a retina, methods of treating ocular degenerative diseases and methods to select cells for transplantation.

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Номер записи: 71
20-09-2012 дата публикации

Generation of Functional Basal Forebrain Cholinergic Neurons From Stem Cells

Номер: US20120237484A1
Автор: Christopher Bissonnette, John Kessler
Принадлежит: Northwestern University

The present invention provides method, compositions, and systems for generating basal forebrain cholinergic neurons (BFCNs) using FGF8, SHH, LXH8, GBX1, or vectors encoding these ligands, as well as using such BFCNs to treat neurological disorders such as Alzheimer's disease.

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Номер записи: 72
20-09-2012 дата публикации

Mesenchymal stem cell and the method of use thereof

Номер: US20120237487A1
Автор: Kazuo Hashi, Osamu Honmou, Teiji Uede
Принадлежит: NC MEDICAL RES Inc

Demyelinated axons were remyelinated in the demyelinated rat model by collecting bone marrow cells from mouse bone marrow and transplanting the mononuclear cell fraction separated from these bone marrow cells.

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Номер записи: 73
20-09-2012 дата публикации

Production of viral vaccines in suspension on avian embryonic derived stem cell lines

Номер: US20120238001A1
Автор: Arnaud Leon, Majid Mehtali, Patrick Champion-Arnaud
Принадлежит: Vivalis SA

The present invention relates to the development and manufacturing of viral vaccines. In particular, the invention relates to the field of industrial production of viral vectors and vaccines, more in particular to the use of avian embryonic stem cells, preferably the EBx® cell line derived from chicken embryonic stem cells, for the production of viral vectors and viruses. The invention is particularly useful for the industrial production of viral vaccines to prevent viral infection of humans and animals.

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Номер записи: 74
20-09-2012 дата публикации

Megakaryocyte and Platelet Production from Stem Cells

Номер: US20120238020A1
Автор: Mauro P. Avanzi, W. Beau Mitchell
Принадлежит: New York Blood Center Inc

Methods for obtaining purified populations of megakaryocytes and platelets by ex vivo culture of stem cells are provided herein.

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Номер записи: 75
20-09-2012 дата публикации

Novel Method for Producing Differentiated Cells

Номер: US20120238023A1
Автор: Hiromitsu Nakauchi, Koji Eto, Naoya Takayama, Sou Nakamura
Принадлежит: Individual

The present invention has an object of providing a method for producing specific cells by amplifying cells in a desired differentiation stage. The present invention provides a method for producing specific cells by inducing differentiation of cells, wherein an oncogene is forcibly expressed in cells in a desired differentiation stage to amplify the cells in the desired differentiation stage. The present invention also provides a method for producing specific cells, wherein oncogene-induced senescence (OIS) which is induced by the oncogene expressed in the cells in the desired differentiation stage is suppressed.

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Номер записи: 76
20-09-2012 дата публикации

Methods for regulating the growth and/or survival of tumor cells and stem cells by modulating the expression or function of the transcription factors atf5

Номер: US20120238462A1
Автор: James M. Angelastro, Lloyd A. Greene
Принадлежит: Individual

The present invention provides methods for regulating the growth and/or survival of tumor cells and stem cells by modulating the expression or function of ATF5.

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Номер записи: 77
27-09-2012 дата публикации

Pluripotent stem cell that can be isolated from body tissue

Номер: US20120244129A1
Автор: Mari Dezawa, Masanori Yoshida, Shohei Wakao, Yasumasa KURODA, Yoshinori Fujiyoshi, Youichi Nabeshima
Принадлежит: Individual

Objects of the present invention are to provide a method for directly obtaining pluripotent stem cells which do not have tumorigenic property from body tissue and the thus obtained pluripotent stem cells. The present invention relates to SSEA-3 (+) pluripotent stem cells that can be isolated from body tissue.

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Номер записи: 78
27-09-2012 дата публикации

Methods of growing tumor infiltrating lymphocytes in gas-permeable containers

Номер: US20120244133A1
Автор: David Stroncek, Jianjian Jin, John R. Wilson, Marianna SABATINO, Mark E. Dudley, Robert Somerville, Steven A. Rosenberg
Принадлежит: US Department of Health and Human Services, Wilson Wolf Manufacturing Corp

An embodiment of the invention provides a method of promoting regression of cancer in a mammal comprising obtaining a tumor tissue sample from the mammal; culturing the tumor tissue sample in a first gas permeable container containing cell medium therein; obtaining tumor infiltrating lymphocytes (TIL) from the tumor tissue sample; expanding the number of TIL in a second gas permeable container containing cell medium therein using irradiated allogeneic feeder cells and/or irradiated autologous feeder cells; and administering the expanded number of TIL to the mammal. Methods of obtaining an expanded number of TIL from a mammal for adoptive cell immunotherapy are also provided.

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Номер записи: 79
27-09-2012 дата публикации

Mesh enclosed tissue constructs

Номер: US20120244617A1
Автор: Arash Kheradvar, Seyedhamed Alavi
Принадлежит: UNIVERSITY OF CALIFORNIA

Described is a scaffold that is strong enough to resist forces that exist inside a body, while possessing biocompatible surfaces. The scaffold is formed of a layer of mesh (e.g., Stainless Steel or Nitinol) that is tightly enclosed by a multi-layer biological matrix. The biological matrix can include three layers, such a first layer (smooth muscle cells) formed directly on the metal mesh, a second layer (fibroblast/myofibroblast cells) formed on the first layer, and a third layer (endothelial cells) formed on the second layer. The scaffold can be formed to operate as a variety of tissues, such as a heart valve or a vascular graft. For example, the mesh and corresponding biological matrix can be formed as leaflets, such that the scaffold is operable as a tissue heart valve.

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Номер записи: 80
27-09-2012 дата публикации

Use of pedf-derived polypeptides for promoting stem cells proliferation and wound healing

Номер: US20120245097A1
Автор: Tsung-Chuan Ho, Yeou-Ping Tsao
Принадлежит: MACKAY MEMORIAL HOSPITAL

Disclosed herein is a synthetic peptide, which has an amino acid sequence that has 20-39 amino acid residues. The synthetic peptide has at least 80% amino acid sequence identity to SEQ ID NO: 1, and includes at least 20 consecutive residues that has at least 90% amino acid sequence identity to residues 11-30 of SEQ ID NO: 1. Also disclosed herein are compositions containing the synthetic peptide and applications thereof. According to various embodiments of the present disclosure, the synthetic peptide is useful in promoting stem cells proliferation or wound healing.

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Номер записи: 81
04-10-2012 дата публикации

HUMAN LYMPHOID TISSUE INDUCER (LTi) CELL COMPOSITIONS AND METHODS OF USE

Номер: US20120251505A1
Автор: Carl F. Ware, Vasileios Bekiaris
Принадлежит: La Jolla Institute for Allergy and Immunology

The invention provides human lymphoid tissue inducer (LTi) cells, methods of producing human lymphoid tissue inducer (LTi) cells, and methods of using human lymphoid tissue inducer (LTi) cells. Such methods include treatment of a subject that would benefit from human lymphoid tissue inducer (LTi) cells, for example, an immunocompromised or immunosuppressed subject.

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Номер записи: 82
04-10-2012 дата публикации

Methods and compositions relating to neuronal cell and tissue differentiation

Номер: US20120251506A1
Автор: Bradley J. Molyneaux, Jeffrey D. Macklis, Paola Arlotta
Принадлежит: General Hospital Corp

The invention relates to methods for isolating and purifying specific types of neurons, such as cortical or other projection neurons including corticospinal motor neurons, subcerebral projection neurons, and callosal projection neurons. The invention also relates to genes that are specific for particular neuronal subtypes, and the use of such genes in genetic/molecular control of cell development. The isolated cells and subtype-specific genes also have uses in diagnostics, therapeutics, and screening assays for pharmaceutical molecules.

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Номер записи: 83
04-10-2012 дата публикации

Methods of generating a tendon tissue

Номер: US20120253463A1
Автор: Joseph Itskovitz-Eldor, Shahar Cohen
Принадлежит: Technion Research and Development Foundation Ltd

Methods of generating and expanding proliferative, multipotent connective tissue progenitor cells from adult stem cells are provided. Also provided are methods of generating functional tendon grafts in vitro and bone, cartilage and connective tissues in vivo using the isolated cell preparation of connective tissue progenitor cells.

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Номер записи: 84
11-10-2012 дата публикации

Production of low fucose antibodies in h4-ii-e rat cells

Номер: US20120258496A1
Автор: Hitto Kaufmann, Kristina Ellwanger, Lore Florin
Принадлежит: BOEHRINGER INGELHEIM INTERNATIONAL GMBH

The invention concerns the field of cell culture technology. It specifically concerns a rat hepatoma cell, preferably a H4-II-E rat hepatoma cell, carrying a DNA encoding an antibody or Fc-fusion protein and having low fucosylation activity for adding fucose to glycosidic structures such as biantennary glycans, e.g. N-acetylglucosamine. The invention furthermore concerns a method for producing low fucose glycoproteins especially antibodies or Fc-fusion proteins in rat hepatoma cells, preferably in H4-II-E rat hepatoma cells. It further concerns the identification and generation of new host cell lines which are capable of synthetizing glycoproteins with beneficial properties, improving the therapeutic efficacy and/or serum half-life of the product compared to products from commonly used host cell lines.

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Номер записи: 85
18-10-2012 дата публикации

Induction of pluripotent cells

Номер: US20120264218A1
Автор: Sheng Ding, Tongxiang Lin
Принадлежит: Scripps Research Institute

The slow kinetics and low efficiency of reprogramming methods to generate human induced pluripotent stem cells (iPSCs) impose major limitations on their utility in biomedical applications. Here we describe a chemical approach that dramatically improves (>200 fold) the efficiency of iPSC generation from human fibroblasts, within seven days of treatment. This will provide a basis for developing safer, more efficient, non-viral methods for reprogramming human somatic cells.

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Номер записи: 86
25-10-2012 дата публикации

Productions of artificial tissues by means of tissue engineering using agarose-fibrin biomaterials

Номер: US20120269776A1
Автор: Antonio CAMPOS MUÑOZ, Ingrid Johanna Garzón Bello, José Ignacio Muñoz Ávila, Miguel Alaminos Mingorance, Miguel GONZÁLEZ ANDRADES
Принадлежит: SERVICIO ANDALUZ DE SALUD, Universidad de Granada

The present invention is encompassed in the field of biomedicine and more specifically tissue engineering. It relates specifically to an in vitro method for preparing an artificial tissue, to the artificial tissue obtainable by said method and to the use of this artificial tissue to partially or completely increase, restore or replace the functional activity of a damaged tissue or organ.

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Номер записи: 87
25-10-2012 дата публикации

Method for proliferating hair follicle stem cells

Номер: US20120269781A1
Автор: Eun Ji Yoon, Jeong Chan Ra, Mi Ae Kim, Sang Kyu Woo, Sung Keun Kang
Принадлежит: RNL Bio Co Ltd

The present invention relates to a method of proliferating follicular stem cells in high yield, and more particularly, to a method of proliferating follicular stem cells in large amounts by culturing the cells using a specific medium containing a specific concentration of a Rho-associated kinase (ROCK) inhibitor and to a medium which is used in the method.

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Номер записи: 88
25-10-2012 дата публикации

Method for preparing a substrate for arranging animal cells in an array and method for preparing a substrate on which animal cells are arranged in an array

Номер: US20120270755A1
Автор: Sumihiro Koyama
Принадлежит: Japan Agency for Marine Earth Science and Technology

A new means of separately arranging individual animal cells on a substrate surface is provided. A method for preparing a substrate for arranging animal cells in an array, comprising steps (1) to (3): (1) preparing a substrate having adsorption surfaces in an array on an electrode substrate surface; (2) causing an extracellular matrix to adsorb to the electrode surface and the adsorption surfaces of the substrate; and (3) applying a weak potential to the electrode to cause the extracellular matrix on the electrode surface to separate to obtain a substrate with the extracellular matrix adhered to the adsorption surfaces thereof. A method for preparing a substrate on which animal cells have been arranged in an array, comprising steps (1) to (4): (1) preparing a substrate having adsorption surfaces in an array on an electrode substrate surface; (2) causing an extracellular matrix to adsorb to the electrode surface and the adsorption surfaces of the substrate; (3) applying a weak potential to the electrode to cause the extracellular matrix on the electrode surface to separate to obtain a substrate with extracellular matrix adhered to the adsorption surfaces thereof; and (4) culturing the animal cells on the surface of the substrate obtained in (3) to obtain a substrate on which the animal cells have adhered to the adsorption surface.

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Номер записи: 89
25-10-2012 дата публикации

Production of glycoproteins with low n-glycolylneuraminic acid (neu5gc) content

Номер: US20120271041A1
Автор: Tanja Ficko Trcek
Принадлежит: Individual

The present invention relates to a medium for the cultivation of eukaryotic cells, the medium comprising as (an) additive(s) DMSO, N-acetylmannosamine (NAcMan), N-acetylglucosamine (NAcGlc), or any combination of two or more of these additives, including the combination of NAcMan and NAcGlc.

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Номер записи: 90
25-10-2012 дата публикации

Method for constructing chimeric rat using rat embryonic stem cells

Номер: US20120272349A1
Автор: Masaki Kawamata, Takahiro Ochiya
Принадлежит: DS Pharma Biomedical Co Ltd, National Cancer Center Japan

The present invention provides a preparation method of a chimeric embryo and a chimeric rat, which is characterized by contacting a rat pluripotent stem cell and a host embryo in the presence of an ES cell differentiation inhibitor. The method includes (a) a step for contacting a fertilized host embryo collected from a female rat and a rat pluripotent stem cell in the presence of an ES cell differentiation suppressant, and (b) a step for culturing the host embryo in contact with the rat pluripotent stem cell to form a chimeric embryo.

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Номер записи: 91
01-11-2012 дата публикации

Nanotube structures, methods of making nanotube structures, and methods of accessing intracellular space

Номер: US20120276573A1
Автор: Alexander M. Xu, Jules J. VanDersarl, Nicholas A. Melosh
Принадлежит: Leland Stanford Junior University

In accordance with the purpose(s) of the present disclosure, as embodied and broadly described herein, embodiments of the present disclosure, in one aspect, relate to methods of making a structure including nanotubes, a structure including nanotubes, methods of delivering a fluid to a cell, methods of removing a fluid to a cell, methods of accessing intracellular space, and the like.

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Номер записи: 92
01-11-2012 дата публикации

Cell surface coating with hyaluronic acid oligomer derivative

Номер: US20120277416A1
Автор: Alexander Tuzikov, Deborah Adella Blake, Eleanor Christine Williams, Elena Yurievna Korchagina, Nicola Lewell Carter, Nicolai Bovin, Stephen Michael Henry
Принадлежит: Kode Biotech Ltd

A method of localising reproduction assisting hyaluronic acid to reproductive cell surfaces by covalently linking it to lipids is disclosed.

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Номер записи: 93
08-11-2012 дата публикации

Non-viral delivery of transcription factors that reprogram human somatic cells into a stem cell-like state

Номер: US20120282229A1
Автор: Christian Kannemeier, Joel Sae Won Marh, John Sundsmo, Kyle Howerton
Принадлежит: Individual

Disclosed herein are cellular compositions, stable continuous cell cultures, reporter cell lines, pharmaceutical preparations, cell penetrable pluripotent stem cells transcription factors and methods related thereto, related to reprogrammed somatic cells.

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Номер записи: 94
08-11-2012 дата публикации

Nanoparticle loaded stem cells and their use in mri guided hyperthermia

Номер: US20120283503A1
Автор: Lyubov Ostrovska
Принадлежит: JOHNS HOPKINS UNIVERSITY

The present invention provides stem cells loaded with bi-functional magnetic nanoparticles (nanoparticle-loaded stem cells (NLSC)) that both: a) heat in an alternating magnetic field (AMF); and b) provide MRI contrast enhancement for MR-guided hyperthermia. The nanoparticles in the NLSC are non-toxic, and do not alter stem cell proliferation and differentiation, the nanoparticles do however, become heated in an alternating magnetic field, enabling therapeutic applications for cancer treatment. NLSC can deliver hyperthermia to hypoxic areas in tumors for sensitization of those areas to subsequent treatment, thus delivering therapy to the most treatment-resistant tumor regions. The heating of diseased tissue either results in direct cell killing or makes the tumor more susceptible to radio- and/or chemotherapy. The NLSC of the present invention can be used for MR image-guided hyperthermia in oncology, in stem cell research for cell tracking and heating, and for elimination of mis-injected stem cells.

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Номер записи: 95
22-11-2012 дата публикации

Method for proliferating stem cells by activating c-met/hgf signaling and notch signaling

Номер: US20120295348A1
Автор: Bong Gu Kang, Do Hyun Nam, Kyeung Min Joo, Seung Chyul Hong
Принадлежит: SAMSUNG LIFE PUBLIC WELFARE FOUNDATION

The present invention relates to stem cells in which a gene that activates signaling is introduced and to a method for proliferating the stem cells. More specifically, the invention relates to a method of significantly increasing the ability of stem cells to proliferate, either by transfecting stem cells with the Notch intracellular domain (NICD) to activate the Notch signaling pathway, or by transfecting stem cells with the c-MET gene and treating the transfected stem cells with the HGF ligand protein to activate the c-MET/HGF signaling pathway. According to the present invention, as a result of activating the Notch signaling pathway or the c-MET/HGF signaling pathway, stem cells having an excellent ability to proliferate can be produced in large amounts. Particularly, since neural stem cells which have been difficult to culture in vitro can be proliferated in large amounts, thus the neural stem cells will be more useful for the preparation of cell therapeutic agents for treating cranial nerve diseases.

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Номер записи: 96
29-11-2012 дата публикации

Dac hyp compositions and methods

Номер: US20120301429A1
Автор: Braeden Partridge, J. Yun Tso, John E. Burky, Mark C. Wesson, Paul W. Sauer, Ping Y. Huang, Taymar E. Hartman, Thomas J. Robinson
Принадлежит: AbbVie Biotherapeutics Inc

The present disclosure relates to compositions of daclizumab suitable for subcutaneous administration and methods of manufacturing thereof.

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Номер записи: 97
29-11-2012 дата публикации

Technologies, Methods, and Products of Small Molecule Directed Tissue and Organ Regeneration from Human Pluripotent Stem Cells

Номер: US20120301437A1
Автор: Xuejun Huang Parsons
Принадлежит: SAN DIEGO REGENERATIVE MEDICINE INST

Pluripotent human embryonic stem cells (hESCs) hold great potential for restoring tissue and organ function, which has been hindered by inefficiency and instability of generating desired cell types through multi-lineage differentiation. This instant invention is based on the discovery that pluripotent hESCs maintained under defined culture conditions can be uniformly converted into a specific lineage by small molecule induction. Retinoic acid induces specification of neuroectoderm direct from the pluripotent state of hESCs and triggers progression to neuronal progenitors and neurons efficiently. Similarly, nicotinamide induces specification of cardiomesoderm direct from the pluripotent state of hESCs and triggers progression to cardiac precursors and cardiomyocytes efficiently. This technology provides a large supply of clinically-suitable human neuronal or cardiac therapeutic products for CNS or myocardium repair. This invention enables well-controlled efficient induction of pluripotent hESCs exclusively to a specific clinically-relevant lineage for tissue and organ engineering and regeneration, cell-based therapy, and drug discovery.

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Номер записи: 98
29-11-2012 дата публикации

Treatment of bone fracture

Номер: US20120301442A1
Автор: Simon Cool, Victor Nurcombe
Принадлежит: Agency for Science Technology and Research Singapore

The use of mesenchymal stem cells cultured in the presence of HS-2 for the treatment of bone fracture. Repair of bone fracture using such cells is enhanced compared with the treatment of bone fracture using mesenchymal cells cultured without HS-2. These mesenchymal stem cells may be formulated in a pharmaceutical composition and injected directly into tissues surrounding the fracture or used in a biocompatible implant or prosthesis.

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Номер записи: 99
06-12-2012 дата публикации

Antibodies specific for claudin 6 (cldn6)

Номер: US20120308478A1
Автор: Bernd Hubner, Korden Walter, Maria Kreuzberg, Michael Erdeljan, Michael Koslowski, Özlem TÜRECI, Stefan WÖLL, Ugur Sahin
Принадлежит: Bernd Hubner, Korden Walter, Maria Kreuzberg, Michael Erdeljan, Michael Koslowski, Özlem TÜRECI, Stefan WÖLL, Ugur Sahin

The present invention provides antibodies useful as therapeutics for treating and/or preventing diseases associated with cells expressing Claudin-6 (CLDN6), including tumor-related diseases such as ovarian cancer, lung cancer, gastric cancer, breast cancer, hepatic cancer, pancreatic cancer, skin cancer, malignant melanoma, head and neck cancer, sarcoma, bile duct cancer, cancer of the urinary bladder, kidney cancer, colon cancer, placental choriocarcinoma, cervical cancer, testicular cancer, and uterine cancer.

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Номер записи: 100