BEWERTUNG DER KNOCHENZELLAKTIVITÄT

Zellkulturträger, Verfahren zum Herstellen von Zellkulturträgern und Verfahren zur Zellkultivierung

Beschrieben sind Zellkulturträger, auf denen Zellen effizient anhaften und wachsen und von denen die gewachsenen Zellen einfach gelöst werden, können, ein Verfahren zum einfachen und zuverlässigen Herstellen solcher Zellkulturträger sowie ein Verfahren zur Zellkultivierung unter Verwendung solcher Zellkulturträger. Die Zellkulturträger enthalten jeweils ein körniges Grundmaterial und eine Deckschicht, die die Oberfläche des Grundmaterials abdeckt. Die Deckschicht besteht hauptsächlich aus einem Calciumphosphat-basierten Apatit, bei dem ein Teil des Calciums fehlt. Solche Zellkulturträger werden in Zellkulturen verwendet, in denen die Zellen auf den Oberflächen der Zellkulturträger haften und dort wachsen. Insbesondere werden diese Zellkulturträger in dreidimensionalen Kulturen hoher Dichte (Suspensionskulturen) verwendet. Der Ca-Mangel in dem Calciumphosphat-basierten Apatit liegt vorzugsweise in einem Bereich von 1 bis 30 Mol-%.

Novel Microcarrier beads

A microcarrier bead made from apatite characterised by one or more of the following: (a) micro-metre sized; (b) regular porous structure; (c) rough surface; (d) substantially spherical; (e) osteo-conductivity; (f) chemical similarity to the mineral phase of bone; and (g) high thermal stability. Preferably the apatite is selected from: hydroxyapatite, silicon-substituted apatite, silver-substituted apatite, magnesium-substituted apatite and a stoichiometric apatite which is a synthetic apatite with a Ca/P atomic ratio that approaches 1.67. Preferably the apatite is phase-pure and the beads are between 100-800µm diameter. A method for making microcarrier beads is outlined comprising (a) mixing apatite and alginate in a solution and allowing them to disperse to form a suspension; (b) extruding the suspension dropwise through a droplet device; (c) exposing the extruded droplets to a calcium chloride solution and dispersing the same; (e) hardening the beads in a solution of alcohol; (f) drying the beads; and (g) sintering the beads to burn off the alginate. Preferably the apatite microcarrier beads have attached thereto or grown thereon at least one cell, selected from stem cells, progenitor cells or induced pluripotent stem cells, or a tissue type. Preferably the beads are for use in the repair of craniomaxillofacial defects, wrist fracture, spinal fusion procedures and periodontal defects.

Carrier for cell culture and method for culturing cells

A carrier for cell culture which enables cells to efficiently grow thereon is provided. A carrier 1 comprises a particulate base body 2 mainly formed of a resin material and a coating layer 3. The coating layer 3 is formed from particles 31 of a calcium phosphate-based compound, and the particles 31 are partially embedded in the base body 2 at the vicinity of the surface thereof whereby the coating layer 3 coats the surface of the base body 2 with a calcium phosphate-based compound. Such a carrier 1 enables cells to adhere to and grow on the surface thereof. Therefore, the carrier 1 can be used for cell culture, especially high-density three-dimensional cell culture. The coating layer 3 can be formed, for example, by colliding porous particles of a calcium phosphate-based compound against the surface of the base body 2.

CALCIUM-CONTAINING STRUKTURE AND PROCEDURES FOR THE PRODUCTION AND USE OF IT

ADJUSTMENT OF THE HÄMATOPOIETI MASTER CELL DIFFERENTIATION BY USE OF HUMAN MESENCHYMALEN MAIN CELLS

CELLS, CULTURAL PROCEDURES, AND YOUR USE IN AUTOLOGER TRANSPLANT ION THERAPY

Assessment of bone cell activity

CELL CONSTRUCTS CULTURED IN VITRO, PREPARATION AND USES

ACTIVATION AND EXPANSION OF T CELLS

The present invention relates generally to methods for activating and expanding T cells, and more particularly, to a novel method to activate and/or stimulate T cells that maximizes the expansion of such cells to achieve dramatically high densities. In the various embodiments, cells are activated and expanded to very high densities in a short period of time. In certain embodiments, cells are activated and expanded to very high numbers of cells in a short period of time. Compositions of cells activated and expanded by the methods herein are further provided.

ACTIVATION AND EXPANSION OF CELLS

The present invention relates generally to methods for activating and expanding T cells, and more particularly, to a novel method to activate and/or stimulate T cells that maximizes the expansion of such cells to achieve dramatically high densities. In the various embodiments, cells are activated and expanded to very high densities in a short period of time. In certain embodiments, cells are activated and expanded to very high numbers of cells in a short period of time. Compositions of cells activated and expanded by the methods herein are further provided.

NEOMORPHOGENSIS OF CARTILAGE IN VIVO FROM CELL CULTURE

Methods and artificial matrices for the growth and implantation of cartilaginous structures and surfaces and bone are dis-closed. In the preferred embodiments, chondrocytes are grown on biodegradable, biocompatible fibrous polymeric matrices. Op-tionally, the cells are proliferated in vitro until an adequate cell volume and density has developed for the cells to survive and pro-liferate in vivo. One advantage of the matrices is that they can be cast or molded into a desired shape, on an individual basis, so that the final product closely resembles a patient's own ear or nose, Alternatively, flexible matrices can be used which can be ma-nipulated at the time of implantation, as in a joint followed by remodeling through cell growth and proliferation in vivo. The cul-tured cells can also be maintained on the matrix in a nutrient media for production of bioactive molecules such as angiogenesis inhibiting factor, Examples are provided showing the growth of hyaline cartilage for joint relinings, the growth of elastic cartia-lage for plastic or reconstructive replacement of cartilage structures, and repair of large bone defects.

METHOD FOR THE IN VITRO PRODUCTION OF BONE

The invention relates to a method for in vitro production of bone tissue, comprising the steps of: (a) applying undifferentiated mammalian cells, in particular autologous marrow cells, on a substrate. (b) directly contacting said cells with a culture for a sufficient time to produce a continuous matrix; (c) removing the substrate with the matrix from the culture medium. The produced matrix can be used for joint prostheses, maxillofacial implants, special surgery devices, or bone fillers. The contacted culture medium can also be used for the production of active factors such as growth factors.

СПОСОБ И УСТРОЙСТВО ДЛЯ ПОЛУЧЕНИЯ БИОЛОГИЧЕСКОЙ ТКАНИ В КАМЕРЕ РОСТА

Изобретение относится к способу и устройству для получения ткани в камере роста для трансплантации внутрь или на поверхность тела человека или животного. Одно из особых преимуществ изобретения состоит в том, что оно обеспечивает получение структурно функциональной кости. По изобретению биологические клетки размещают на каркасе роста и располагают вместе в камере роста. Структурный и функциональный рост получают при помощи целенаправленного, различающегося по времени или месту действия биологически активных стимулов. Упомянутые стимулы могут быть механическими, электрическими, магнитными, химическими, обонятельными, звуковыми и/или оптическими. По изобретению наиболее предпочтительно применение стимулов, аналогичных таким, которые обычно воздействуют на природную ткань в теле.

Cell constructs cultured in vitro, preparation and uses

Biocompatible implant for expression in humans or in animals given substances.

SUPPORT FOR CULTURE OF CELLS AND A METHOD FOR CULTIVATING CELLS.

L'invention concerne un support (1) pour culture cellulaire, comprenant un corps de base sous forme de particules (2) et une couche de revêtement (3).Le corps de base est formé d'une résine et la couche de revêtement est formée de particules (31) d'un composé à base de phosphate de calcium partiellement incluses dans le corps de base (2) à proximité de sa surface. APPLICATION : Utilisation de ce support pour un procédé de culture cellulaire, notamment de culture cellulaire tridimensionnelle haute densité.

PROCEEDED FOR the PRODUCTION Of ONE CALCINING COMPRESSES, COMPRESSES CALCINING PRODUCED PROCEEDED PARCE, AND BASES CELLULAR CULTURE FROM THIS COMPRESSES CALCINING

L'invention concerne un procédé pour la production d'un comprimé fritté en moulant un comprimé cru par compactage d'une poudre d'hydroxyapatite et en frittant le comprimé cru dans une atmosphère contenant de l'oxygène, puis à soumettre le comprimé fritté à un frittage secondaire dans une atmosphère à faible taux d'activité.Elle concerne également le comprimé fritté produit par ce procédé.APPLICATION :Production d'un support ou d'une base pour culture cellulaire.

Novel Microcarrier Beads

Novel Microcarrier 5 The invention relates to a novel microcarrier bead; a method for producing same; a therapeutic comprising said microcarrier bead and attached thereto or grown thereon at least one selected cell or tissue type; a method for making said therapeutic; and method a of treatment involving the use of said microcarrier bead or said therapeutic. 10 (Figure 1) 27 ...

BIODEGRADABLE SYNTHETIC BONE COMPOSITES

The invention provides for a biodegradable synthetic bone composition comprising a biodegradable hydrogel polymer scaffold comprising a plurality of hydro lyrically unstable linkages, and an inorganic component; such as a biodegradable poly(hydroxyethylmethacrylate)/hydroxyapatite (pHEMA/HA) hydrogel composite possessing mineral content approximately that of human bone.

CELLS, CULTURE METHODS, AND THEIR USE IN AUTOLOGOUS TRANSPLANTATION THERAPY

L'invention porte sur l'utilisation d'une population de cellules hôtes provenant d'un organisme hôte sain pour préparer une composition de cellules et pouvant servir à des thérapies de transplantation autologues ultérieures sur ledit organisme hôte.

Structures useful for bone engineering and methods

A bone repair apparatus is provided where a biologically compatible structure has a compound carried on the structure that mimics collagen binding to cells. Living cells derived from fibroblasts are carried on the structure and display at least one morphologic change consistent with an osteogenic phenotype. The preferred method for practicing the invention includes harvesting a quantity of fibroblasts from a patient in need of a bone graft, growing the tissue under cell growth conditions, and seeding at least some cells of the cultured tissue on the biologically compatible structure with the collagen mimic thereon. The culture tissue cells are seeded on the structure and incubated under cell growth conditions, which results in the differentiation of the cells to bone-like cells and thus provides a tissue engineered apparatus ready for use as a bone graft.

Porous composite materials

Methods and compositions are described that provide three dimensional porous matrices as structural templates for cells. The porous matrices of the present invention have desirable mechanical properties suitable to a variety of applications, including platforms for in vitro cell cultivation, implants for tissue and organ engineering, and materials suitable for chromatography and filtration.

Activation and expansion of cells

The present invention relates generally to methods for activating and expanding cells, and more particularly, to a novel method to activate and/or stimulate cells that maximizes the expansion of such cells to achieve dramatically high densities. In the various embodiments, cells are activated and expanded to very high densities in a short period of time. In certain embodiments, cells are activated and expanded to very high numbers of cells in a short period of time. Compositions of cells activated and expanded by the methods herein are further provided.

PRODUCTION OF DENTIN, CEMENTUM AND ENAMEL BY CELLS

CELLS, CULTURE METHODS, AND THEIR USE IN AUTOLOGOUS TRANSPLANTATION THERAPY

Carrier for cell culture and method for culturing cells

INTO VIVO NEOMORPHOGENESE ONES OF CARTILAGE FROM CELL CULTURES

THERMALLY STERILIZE-CASH OF POROUS CARRIER BODIES FOR BIOCATALYSTS

THE RE-ESTABLISHMENT AND REINFORCEMENT OF BONES OF MEANS MESENCHYMALEN MAIN CELLS

Methods and materials for nanocrystalline surface coatings and attachment of peptide amphiphile nanofibers thereon

Simultaneous stimulation and concentration of cells

Assessment of bone cell activity

CELL CONSTRUCTS CULTURED IN VITRO, PREPARATION AND USES

La présente invention concerne un construit cellulaire cultivé in vitro caractérisé en ce qu'il comprend (i) des cellules animales cultivées in vitro dans des conditions assurant la formation d'une structure tissulaire tridimensionnelle et (ii) une matrice extra-cellulaire endogène dans laquelle lesdites cellules sont emprisonnées, et en ce qu'il comprend, parmi lesdites cellules, des cellules ayant une capacité de minéralisation et/ou d'ossification. L'invention est particulièrement utile dans le domaine des greffes ou de l'implantologie (reconstitution ou réparation tissulaire), ainsi que dans l'industrie pharmaceutique, pour l'étude des tissus et l'analyse du profil toxique ou bénéfique de molécules susceptibles d'entrer en développement pharmaceutique et/ou dans des compositions pharmaceutiques.

ACTIVATION AND EXPANSION OF CELLS

The present invention relates generally to methods for activating and expanding T cells, and more particularly, to a novel method to activate and/or stimulate T cells that maximizes the expansion of such cells to achieve dramatically high densities. In the various embodiments, cells are activated and expanded to very high densities in a short period of time. In certain embodiments, cells are activated and expanded to very high numbers of cells in a short period of time. Compositions of cells activated and expanded by the methods herein are further provided.

COMPOSITIONS COMPRISING CITRATE AND APPLICATIONS THEREOF

In one aspect, methods of promoting bone growth are described herein. In some embodiments, a method of promoting bone growth described herein comprises promoting cell differentiation or phenotype progression in a population of bone cells by providing a citrate- presenting composition to the population of bone cells. In some embodiments, the citrate- presenting composition is provided to the bone cells at a first stage of cell development selected to obtain a first cell differentiation or phenotype progression. Additionally, in some cases, a second citrate-presenting composition is further provided to the bone cells at a second stage of cell development selected to obtain a second cell differentiation or phenotype progression.

FUNCTIONALIZED CALCIUM PHOSPHATE ARTIFICIAL BONE AND JOINT COMPOSITIONS AND METHODS OF USE AND MANUFACTURE

A functionalized composition and resulting functionalized body or prosthesis for in vitro and in vivo use comprising at least one calcium phosphate containing phase that is functionalized with a linker group comprising at least one of an organic acid molecule, a phosphoric acid, an amine, N,N-dicyclohexylcarbodiimide, and 3-maleimidopropionic acid N-hydroxysuccinimide ester, wherein the linker group provides for a reactive location for the attachment of one or more of a chemical and/or a biologically active moiety to the calcium phosphate containing phase. The functionalized compositions have a pliable physical property when mixed with water and adhere to natural bone and subsequently harden at ambient temperatures. Methods of manufacturing a functionalized artificial prosthesis that may be used as artificial bones, joints, in-vitro support structures, and in-vivo support structures for cells, tissues, organs, and nerve growth and regeneration are disclosed. Methods of repairing a bone, ...

USE OF PARTICLES OF A BIOCOMPATIBLE, BIOABSORBABLE CALCIUM SALT AS ACTIVE PRINCIPLE IN THE PREPARATION OF A MEDICAMENT FOR LOCAL TREATMENT OF BONE DEMINERALIZING DISEASES

Utilisation d'au moins un sel calcium biocompatible et biorésorbable, sous forme de particules, ayant des dimensions inférieures à 8 mm, comme ingrédients actif dans la préparation d'un médicaments destiné a u traitement local, par implantation dans la partie spongieuse ou le canal médullaire de l'os, des maladies liées à la déminéralisation ou à d es troubles de minéralisation de l'os, en particulier dans le but de relancer le processus de reminéralisation de l'os et de reconstruction des travées osseuses résorbées. Par l'exemple, chez les maladies atteints d'ostéoporose, ce traitement permet d'observer une relance du processus de reminéralisation et la reconstruction des travées osseuses, avec une augmentation très importante de la masse osseuse de l'os traité.

METHOD AND DEVICE FOR PRODUCING BIOLOGICAL TISSUE IN GROWTH CHAMBER

Biocompatible implant for the expression in man or in animals of specific substances

La présente demande de brevet a pour objet un implant caractérisé en ce qu'il comprend un support d'origine biologique permettant l'ancrage biologique de cellules préalablement mises en contact avec des constituants capables d'induire et/ou de favoriser leur gélification, lesdites cellules étant choisies pour leur capacité à exprimer et à sécréter naturellement ou après recombinaison un composé déterminé, par exemple un composé ayant un intérêt thérapeutique.

SUPPORTS OF CELLULAR CULTURE, PROCEEDED FOR THE PRODUCTION OF SUPPORTS DECULTURE CELLULAR AND METHOD FOR CULTIVATING CELLS

L'invention concerne des supports de culture cellulaire (1) ayant au moins une surface constituée d'apatite à base de phosphate de calcium dont une partie du Ca manque, et un procédé pour leur production.Ils comprennent une matière de base (2) constituée d'une résine pouvant contenir une matière magnétique, et une couche de revêtement (3) constituée d'apatite à base de phosphate de calcium dont une partie du Ca manque.Domaine d'application : Méthode de culture de cellules en mettant en contact les cellules avec lesdits supports de culture cellulaire. The invention relates to cell culture carriers (1) having at least one surface made of calcium phosphate apatite of which part of the Ca is lacking, and a method for their production. They comprise a base material (2). consisting of a resin which may contain a magnetic material, and a coating layer (3) consisting of apatite based on calcium phosphate, part of which is missing Ca. Field of application: Method of culturing cells by bringing into contact cells with said cell culture media.

COMPOSITIONS COMPRISING CITRATE AND APPLICATIONS THEREOF

In one aspect, methods of promoting bone growth are described herein. In some embodiments, a method of promoting bone growth described herein comprises promoting cell differentiation or phenotype progression in a population of bone cells by providing a citrate- presenting composition to the population of bone cells. In some embodiments, the citrate- presenting composition is provided to the bone cells at a first stage of cell development selected to obtain a first cell differentiation or phenotype progression. Additionally, in some cases, a second citrate-presenting composition is further provided to the bone cells at a second stage of cell development selected to obtain a second cell differentiation or phenotype progression.

TOOTH SCAFFOLDS

Provided is an acellular mammalian tooth-shaped scaffold comprising a compound that is chemotactic, osteogenic, dentinogenic, amelogenic, or cementogenic. Also provided is a method of replacing a tooth in the mouth of a mammal, where the tooth is absent and a tooth socket is present in the mouth at the position of the absent tooth. The method comprises implanting an acellular scaffold having the shape of the missing tooth into the tooth socket. Additionally, a method of making a tooth scaffold is provided. The method comprises synthesizing an acellular scaffold in the shape of a mammalian tooth and adding at least one compound that is chemotactic, osteogenic, dentinogenic, amelogenic, or cementogenic.

Neomorphogenesis of cartilage in vivo from cell culture

Methods and artificial matrices for the growth and implantation of cartilaginous structures and surfaces are disclosed. In the preferred embodiments, chondrocytes are grown on biodegradable, biocompatible fibrous polymeric matrices. Optionally, the cells are proliferated in vitro until an adequate cell volume and density has developed for the cells to survive and proliferate in vivo. One advantage of the matrices is that they can be cast or molded into a desired shape, on an individual basis, so that the final product closely resembles a patient's own ear or nose. Alternatively, flexible matrices can be used which can be manipulated at the time of implantation, as in a joint, followed by remodeling through cell growth and proliferation in vivo. The cultured cells can also be maintained on the matrix in a nutrient media for production of bioactive molecules such as angiogenesis inhibiting factor.

Biomimetic calcium phosphate implant coatings and methods for making the same

This invention encompasses porous, nanocrystalline, biomimetic calcium phosphate coatings of the order of 2-30 microns that can be grown on metal implants. The chemical surface treatments and methods for making the calcium phosphate coatings are disclosed. Post treatment with dilute hydrogels such as phema reinforce the inorganic structure and enhance the mechanical strength of the coatings. Methods are also disclosed for adsorbing or covalently attaching growth factor proteins to derivatives of the hydrogel coated calcium phosphate coatings. Such hydrogel reinforced calcium phosphate coatings show equivalent bone tissue growth as the currently used implants and are easily resorbed. This property in combination with the immobilized growth factors is expected to enhance the process of osseointegration of the disclosed coatings.

Allografts combined with tissue derived stem cells for bone healing

There is disclosed a method of combining mesenchymal stem cells (MSCs) with a bone substrate. In an embodiment, the method includes obtaining tissue having MSCs together with unwanted cells. The tissue is digested to form a cell suspension having MSCs and unwanted cells. The cell suspension is added to the substrate. The substrate is cultured to allow the MSCs to adhere. The substrate is rinsed to remove unwanted cells. In various embodiments, the tissue is adipose tissue, muscle tissue, or bone marrow tissue. In an embodiment, there is disclosed an allograft product including a combination of MSCs with a bone substrate in which the combination is manufactured by culturing MSCs disposed on the substrate for a period of time to allow the MSCs to adhere to the substrate, and then rinsing the substrate to remove unwanted cells from the substrate. Other embodiments are also disclosed.

Process for identification and isolation of mesenchymal cells with marked bone regenerative ability

The present invention concerns a process for attracting, recruiting and selecting mesenchymal cells with high bone tropism and marked bone regenerative ability, which can be used in the treatment of bone lesions of traumatic and pathological origin, in particular in cases of bone neoplasia.

IN VITRO MODELLING OF HAEMATOPOIETIC STEM CELL MEDULLARY NESTS: A TOOL FOR STUDYING THE REGULATION OF HAEMATOPOIESIS, EVALUATING THE NESTING POTENTIAL OF A HAEMATOPOIETIC GRAFT AND TESTING THE PHARMACOTOXICOLOGY OF MEDICAMENTS

Verfahren zur in vitro Herstellung von Knochen

Carrier for cell culture and method for culturing cells

Cell culture carriers,method for manufacturing cell culture carriers and method for culturing cells

A method of preparing microcarrier beads

SIMULTANEOUS STIMULATION AND CONCENTRATION OF CELLS

IN VITRO ZELLKULTUR SYSTEM AND PROCEDURE.

USE OF A POROUS MATERIAL FROM CALCIUM CARBONATE AS CARRIERS FOR A GROWTH FACTOR IN THE PRODUCTION OF AN ABSORBABLE IMPLANT

USE OF POROUS CALCIUM CARBONATE AS SUBSTRATE FOR IN VITRO CELL CULTURES.

EVALUATION OF THE BONE CELL ACTIVITY

PROCEDURE FOR IN VITRO PRODUCTION OF BONES

THERMALLY STERILIZE-CASH OF POROUS OF TRAEGERKOERPER FOR BIOCATALYSTS

Methods and compositions for treatment of bone defects with placental cell populations

Provided herein are methods of using adherent placental stem cells and placental stem cell populations, and methods of culturing, proliferating and expanding the same. Also provided herein are methods of differentiating the placental stem cells. Further provided herein are methods of using the placental stem cells to formulate implantable or injectable compositions suitable for administration to a subject. Still further provided herein are provides methods for treating bone defects with stem cells and compositions comprising stem cells.

Compositions comprising citrate and applications thereof

In one aspect, methods of promoting bone growth are described herein. In some embodiments, a method of promoting bone growth described herein comprises promoting cell differentiation or phenotype progression in a population of bone cells by providing a citrate- presenting composition to the population of bone cells. In some embodiments, the citrate- presenting composition is provided to the bone cells at a first stage of cell development selected to obtain a first cell differentiation or phenotype progression. Additionally, in some cases, a second citrate-presenting composition is further provided to the bone cells at a second stage of cell development selected to obtain a second cell differentiation or phenotype progression.

METHOD FOR CULTURING PEARLS

ABSTRACT OF THE DISCLOSURE Nacre coating of a biocompatible nucleating substrate is achieved by contacting such substrate with mantle tissue explant from a mollusca species under cellular growth promoting conditions. Salt containing media are employed with variations as to the other additives, which may include a source of a steroid material, a source of amino acids, a source of metabolizable energy, or other components. After incubation for sufficient time, nacre coated substrates are formed. By appropriate choice of the nucleus, pearls can be produced.

IN VITRO CELL CULTURE SYSTEM

METHODS AND COMPOSITIONS FOR TREATMENT OF BONE DEFECTS WITH PLACENTAL CELL POPULATIONS

Provided herein are methods of using adherent placental stem cells and placental stem cell populations, and methods of culturing, proliferating and expanding the same. Also provided herein are methods of differentiating the placental stem cells. Further provided herein are methods of using the placental stem cells to formulate implantable or injectable compositions suitable for administration to a subject. Still further provided herein are provides methods for treating bone defects with stem cells and compositions comprising stem cells.

METHODS AND COMPOSITIONS FOR TREATMENT OF BONE DEFECTS WITH PLACENTAL CELL POPULATIONS

Provided herein are methods of using adherent placental stem cells and pl acental stem cell populations, and methods of culturing, proliferating and e xpanding the same. Also provided herein are methods of differentiating the p lacental stem cells. Further provided herein are methods of using the placen tal stem cells to formulate implantable or injectable compositions suitable for administration to a subject. Still further provided herein are provides methods for treating bone defects with stem cells and compositions comprisin g stem cells.

METHOD FOR PRODUCING A SINTERED COMPACT, SINTERED COMPACT PRODUCED BY THE METHOD, AND CELL CULTURE BASE FROM THIS SINTERED COMPACT

Support for culture of the cells in a medium free from serum and device of the type column garnidudit support

Un support pour la culture des cellules prolifératives adhérentes dans un milieu exempt de sérum, ledit support contient un matériau de support en un composé phosphate de calcium. Un dispositif du type colonne pour la culture en continu des cellules, ce dispositif contient le support mentionné ci-dessus garnissant ledit dispositif pour faire passer en continu un milieu exempt de sérum à travers le support pour assurer la prolifération en continu et la différenciation des cellules prolifératives adhérentes de sorte qu'une substance utile produite dans les cellules est recueillie d'un éluat provenant dudit support.

IN VITRO MODELING OF THE MEDULLARY NICHES HAS ORIGINAL CELLS HEMATOPOIETIQUES: A TOOL TO STUDY the REGULATION Of the HEMATOPOIESE, TO EVALUATE the POTENTIAL OF NICHAGE Of a GRAFT HEMATOPOIETIQUE AND TO TEST the PHARMACO-TOXICOLOGIE OF DRUGS.

La présente invention concerne un support de culture de cellules souches hématopoïétiques (CSH) et/ou progéniteurs hématopoïétiques (PH), comprenant un biomatériau calcique, des ostéoclastes, des cellules endothéliales et des cellules souches mésenchymateuses (CSM) et/ou des ostéoblastes et/ou adipocytes. La présente invention concerne également une méthode de préparation d'un tel support de culture, et une méthode de culture in vitro de CSH et/ou PH. L'utilisation d'un tel support de culture pour étudier les mécanismes cellulaires impliqués dans l'hématopoïèse et/ou la différenciation des CSH / PH, et/ou pour étudier l'efficacité et/ou la toxicité d'un candidat médicament est également décrite.

Retroviral vector for the preparation of recombinant cells capable of being implanted in vivo for a therapeutic purpose

L'invention concerne un vecteur rétroviral recombinant caractérisé en ce qu'il comprend: . une séquence d'ADN de provirus modifiée de façon telle que: - les gènes gag, pol et env sont délétés au moins en partie, de façon à supprimer leur capacité à intervenir dans la réplication du rétrovirus, - la séquence LTR comporte une délétion dans la séquence U3, telle que la transcription sous forme d'ARNm qu'elle contrôle est réduite de manière significative, par exemple d'au moins 10 fois, et . le vecteur rétroviral recombinant comprenant, en outre, une séquence de nucléotides exogène sous le contrôle d'un promoteur constitutif exogène.

PROCEEDED FOR the PRODUCTION Of ONE CALCINING COMPRESSES, COMPRESSES CALCINING PRODUCED BY CEPROCEDE, AND BASES CELLULAR CULTURE FROM THIS COMPRESSES

L'invention concerne une base de culture cellulaire comprenant une partie sous forme de plaque plate ayant des première et seconde surfaces, dans lesquelles les cellules sont aptes à adhérer, constituée principalement d'un composé à base de phosphate de calcium formant une partie dense.

BIOLOGICALLY DERIVED COMPOSITE TISSUE ENGINEERING

The present application is directed to engineering of tissues, especially composite tissues such as a joint. Various aspects of the application provide tissue modules and methods of fabrication and use thereof. Some embodiments provide a tissue module that can be fabricated to be substantially similar in anatomic internal and external shape as a target tissue. Some embodiments provide a composite tissue module having a plurality of layers, each of which simulate a different tissue (e.g., bone and cartilage of a joint).

Use of porous polycrystalline aragonite as a support material for in vitro culture of cells

A three-dimensional solid support for the in vitro cultivation of cells consists essentially of porous polycrystalline aragonite in the form of a cylinder, sphere or plate. The support may be used to successively grow layers of different cells on the support, and is particularly useful for cultivating endothelial cells, fibroblasts and bone marrow cells.

BIOCOMPATIBLE IMPLANT FOR THE EXPRESSION AND SECRETION IN VIVO OF A THERAPEUTICAL COMPOUND

The invention relates to an implant obtained by assembling in vitro various elements in order to form a neo-organ which is introduced preferably in the peritoneal cavity of the recipient. The implant comprises a biocompatible support intended to the biological anchoring of cells; cells having the capacity of expressing and secreting naturally or after recombination a predetermined compound, for example a compound having a therapeutical interest; and a constituent capable of inducing and/or promoting the geling of said cells. The invention also relates to a kit for the preparation of the implant as well as to a new recombinant retroviral vector comprising a provirus DNA sequence modified in that the genes gag, pol and env have been deleted at least partially so as to obtain a proviral DNA capable of replication. The invention also relates to recombinant cells comprising the new retroviral vector.

VERWENDUNG EINES PORÖSEN MATERIALS AUS KALZIUMCARBONAT ALS TRÄGER FÜR EINEN WACHSTUMSFAKTOR IN DER HERSTELLUNG EINES RESORBIERBAREN IMPLANTATES

Method for producing decalcified hard tissue sample

CELL CULTURING IN SERUM-FREE MEDIA

APATITE WHISKERS AND A METHOD FOR THEIR PREPARATION

THERMISCH STERILISIERBARER PORÖSER TRÄGERKÖRPER FÜR BIOKATALYSATOREN

BIOCOMPATIBLE IMPLANT TO IN VIVO EXPRESSION AND SECRETION OF THERAPEUTIC CONNECTION

HUMAN MESENCHYM MAIN CELLS FROM THAT MARKS AND MONOCLONAL ANTIBODIES SPECIFIC AGAINST THESE CELLS

USE OF PARTICLES OF A BIOCOMPATIBLE AND BIOABSORBABLE OF CALCIUM SALT AS ACTIVE SUBSTANCE IN THE PRODUCTION OF A DRUG FOR RESTAURANTS THE TREATMENT OF DECALCIFYING DISEASES

T-ZELLAKTIVIERUNG AND - EXPANSION

THERMISCH STERILISIERBARER POROESER TRAEGERKOERPER FUER BIOKATALYSATOREN

HEAT-STERILISABLE POROUS SUBSTRATE FOR BIO-CATALYSTS

REGULATION OF HEMATOPOIETIC STEM CELL DIFFERENTIATION BY THEUSE OF HUMAN MESENCHYMAL STEM CELLS

The invention relates to the induction of hematopoietic stem cells to differentiate into osteoclasts by culturing hematopoietic stem cells with human mesenchymal stem cells, and, in a preferred embodiment, using no exogenous cytokines. Differentiation of the mesenchymal stem cells into osteoblasts inhibited the differentiation of hematopoietic stem cells into osteoclasts. In addition, hematopoietic stem cells can be genetically engineered to carry genes of interest particularly for the expression of physiologically active proteins. In the presence of mesenchymal stem cells, the transduced cells carry the new ...

REGENERATION AND AUGMENTATION OF BONE USING MESENCHYMAL STEMCELLS

Disclosed are compositions and methods for augmenting bone formation by administering isolated human mesenchymal stem cells (hMSCs) with a ceramic material or matrix or by administering hMSCs; fresh, whole marrow; or combinations thereof in a resorbable biopolymer which supports their differentiation into the osteogenic lineage. Contemplated is the delivery of (i) isolated, culture-expanded, human mesenchymal stem cells; (ii) freshly aspirated bone marrow; or (iii) their combination in a carrier material or matrix to provide for improved bone fusion area and fusion mass, when compared to the matrix alone. The material or matrix can be a granular ceramic or three-dimensionally formed ceramic implant. The material or matrix can also be a resorbable biopolymer. The resorbable biopolymer is an absorbable gelatin, colagen or cellulose matrix, can be in the form of a powder or sponge, and is preferably a bovine skin-derived gelatin. The implants can be shaped as a cube, cylinder, block or an anatomical site. The compositions and methods can further include administering a bioactive factor such as a synthetic glucocorticoid, like dexamethasone, or a bone morphogenic protein, like BMP-2, BMP-3, BMP-4, BMP-6 and BMP-7.

Use of particles of a biocompatible calcium salt and biorésorbable like active ingredient in the preparation of a drug intended for the local treatment of the demineralizing diseases of the bone

Utilisation d'au moins un sel de calcium biocompatible et biorésorbable, sous forme de particules, ayant des dimensions inférieures à 8 mm, comme ingrédient actif dans la préparation d'un médicament destiné au traitement local, par implantation dans la partie spongieuse ou le canal médullaire de l'os, des maladies liées à la déminéralisation ou à des troubles de minéralisation de l'os, en particulier dans le but de relancer le processus de reminéralisation de l'os et de reconstruction des travées osseuses résorbées. Par exemple, chez les malades atteints d'ostéoporose, ce traitement permet d'observer une relance du processus de reminéralisation et la reconstruction des travées osseuses, avec une augmentation très importante de la masse osseuse de l'os traité. Use of at least one biocompatible and bioresorbable calcium salt, in the form of particles, having dimensions less than 8 mm, as an active ingredient in the preparation of a medicament intended for local treatment, by implantation in the spongy part or the canal bone marrow, diseases related to demineralization or bone mineralization disorders, in particular with the aim of restarting the process of remineralization of the bone and reconstruction of resorbed bone trabeculae. For example, in patients with osteoporosis, this treatment makes it possible to observe a revival of the remineralization process and the reconstruction of the bone trabeculae, with a very significant increase in the bone mass of the treated bone.

Cell construct formed in vitro, useful e.g. in drug testing or production of dental or ligament implants, comprising cells capable of mineralization and/or ossification enclosed in endogenous extracellular matrix

La présente invention concerne un construit cellulaire cultivé in vitro caractérisé en ce qu'il comprend (i) des cellules animales cultivées in vitro dans des conditions assurant la formation d'une structure tissulaire tridimensionnelle et (ii) une matrice extra-cellulaire endogène dans laquelle lesdites cellules sont emprisonnées, et en ce qu'il comprend, parmi lesdites cellules, des cellules ayant une capacité de minéralisation et/ ou d'ossification. L'invention est particulièrement utile dans le domaine des greffes ou de l'implantologie (reconstitution ou réparation tissulaire), ainsi que dans l'industrie pharmaceutique, pour l'étude des tissus et l'analyse du profil toxique ou bénéfique de molécules susceptibles d'entrer en développement pharmaceutique et/ ou dans des compositions pharmaceutiques.

METHODS AND COMPOSITIONS FOR TREATMENT OF BONE DEFECTS WITH PLACENTAL CELL POPULATIONS

Provided herein are methods of using adherent placental stem cells and placental stem cell populations, and methods of culturing, proliferating and expanding the same. Also provided herein are methods of differentiating the placental stem cells. Further provided herein are methods of using the placental stem cells to formulate implantable or injectable compositions suitable for administration to a subject. Still further provided herein are provides methods for treating bone defects with stem cells and compositions comprising stem cells. © KIPO & WIPO 2009 ...

ALLOGRAFTS COMBINED WITH TISSUE DERIVED STEM CELLS FOR BONE HEALING

There is disclosed a method of combining mesenchymal stem cells (MSCs) with a bone substrate. In an embodiment, the method includes obtaining tissue having MSCs together with unwanted cells. The tissue is digested to form a cell suspension having MSCs and unwanted cells. The cell suspension is added to the substrate. The substrate is cultured to allow the MSCs to adhere. The substrate is rinsed to remove unwanted cells. In various embodiments, the tissue is adipose issue, muscle tissue, or bone marrow tissue. In an embodiment, there is disclosed an allograft product including a combination of MSCs with a bone substrate in which the combination is manufactured by culturing MSCs disposed on the substrate for a period of time to allow the MSCs to adhere to the substrate, and then rinsing the substrate to remove unwanted cells from the substrate. Other embodiments are also disclosed.

CALCIUM-CONTAINING STRUCTURES AND METHODS OF MAKING AND USING THE SAME

L'invention se rapporte de manière générale à des structures contenant du calcium et à des procédés de fabrication et d'utilisation de ces structures. Dans un aspect, des microstructures creuses contenant du calcium sont utilisées conjointement avec des tissus/sous-produits osseux pour renforcer des défauts osseux et étendre l'apport de tissus/sous-produits osseux à des fins de renforcement osseux. Des agents de liaison, tels que des ciments de calcium, sont également utilisés dans l'élaboration des microstructures creuses de calcium, seuls ou en combinaison avec des tissus/sous-produits osseux. Les microstructures contenant du calcium présentées dans l'invention sont aussi utiles comme vecteurs de monoxyde d'azote et/ou de composés contenant ou produisant du monoxyde d'azote pour diverses applications in vitro et in vivo. L'invention concerne également des substrats profilés contenant du calcium sur lesquels des cellules/tissus peuvent être cultivés in vitro à des ...

Enhancing hematopoietic progenitor cell engraftment using mesenchymal stem cells

Method and preparations for enhancing hematopoietic progenitor cell engraftment in an individual by administering (i) a culturally expanded mesenchymal stem cell preparation and (ii) hematopoietic progenitor cells. The mesenchymal stem cells are administered in an amount effective to promote engraftment of the hematopoietic progenitor cells.

Biocompatible implant for the expression and in vivo secretion of a therapeutic substance

The invention relates to an implant obtained by assembling in vitro various elements in order to form a neo-organ which is introduced preferably in the peritoneal cavity of the recipient. The implant comprises a biocompatible support intended to the biological anchoring of cells; cells having the capacity of expressing and secreting naturally or after recombination a predetermined compound, for example a compound having a therapeutical interest; and a constituent capable of inducing and/or promoting the gelling of said cells. The invention also relates to a kit for the preparation of the implant as well as to a new recombinant retroviral vector comprising a provirus DNA sequence modified in that the genes gag, pol and env have been deleted at least partially so as to obtain a proviral DNA capable of replication. The invention also relates to recombinant cells comprising the new retroviral vector.

Calcium-containing structures and methods of making and using the same

The present invention generally relates to calcium-containing structures and methods of making and using the structures. In one aspect, hollow calcium containing microstructures are used in conjunction with bone tissues/by-products to augment bone defects and extend the supply of bone tissues/by-products for bone augmentation. Bonding agents, such as calcium cements, are also used in the preparation of the hollow calcium microstructures combined with bone tissues/by-products or for use in preparing the hollow microstructures. The calcium-containing microstructures of the present invention are also useful as delivery vehicles of nitric oxide and/or nitric oxide containing or producing compounds for a variety of in vitro and in vivo uses. Calcium containing contoured substrates upon which cells/tissues can be grown in vitro for replacement and repair of tissues in vivo that conform in size and shape to the tissue surface to be replaced are also provided.

REGENERATION AND AUGMENTATION OF BONE USING MESENCHYMAL STEM CELLS

Disclosed are compositions and methods for augmenting bone formation by administering isolated human mesenchymal stem cells (hMSCs) with a ceramic material or matrix or by administering hMSCs; fresh, whole marrow; or combinations thereof in a resorbable biopolymer which supports their differentiation into the osteogenic lineage. Contemplated is the delivery of (i) isolated, culture-expanded, human mesenchymal stem cells; (ii) freshly aspirated bone marrow; or (iii) their combination in a carrier material or matrix to provide for improved bone fusion area and fusion mass, when compared to the matrix alone. The material or matrix can be a granular ceramic or three-dimensionally formed ceramic implant. The material or matrix can also be a resorbable biopolymer. The resorbable biopolymer is an absorbable gelatin, colagen or cellulose matrix, can be in the form of a powder or sponge, and is preferably a bovine skin-derived gelatin. The implants can be shaped as a cube, cylinder, block or an ...

VERWENDUNG VON TEILCHEN EINES BIOVERTRÄGLICHEN UND BIORESORBIERBAREN KALZIUMSALZES ALS WIRKSTOFF IN DER HERSTELLUNG EINES ARZNEIMITTELS ZUR LOKALEN BEHANDLUNG VON ENTKALKUNGSKRANKHEITEN

Cell culturing in serum-free media

Method for culturing cells, cell culture carriers and cell culture apparatus

Cell constructs cultured in vitro, preparation and uses

Injectable, load-bearing cell/microbead/calcium phosphate bone paste for bone tissue engineering

The invention provides injectable, stem cell-containing calcium phosphate bone pastes for bone tissue engineering and methods of making and using the same.

Production of dentin, cementum and enamel by cells

One aspect provides a method of forming a mineralized material by co-culturing a epithelial cell, such as ameloblasts, and mesenchymal cells, such as osteoblasts or odontoblasts, in a mineral-stimulating medium. Another aspect provides matrix seeded with epithelial cells and mesenchymal cells and infused with a mineral-stimulating medium capable of forming a mineralized material in the matrix. Methods of manufacturing such compositions and methods of treating mineralization-related conditions are also provided.

BLOOD VESSEL MIMIC AND METHOD FOR CULTURING BLOOD VESSEL MIMIC

A method for culturing a blood vessel mimic according to an embodiment of the present invention comprises the steps of: printing a lower structure of a chamber; printing a blood vessel mimic on the lower structure; printing an upper structure of the chamber on the lower structure and the blood vessel mimic; connecting, to both ends of the blood vessel mimic, tubes connected to a circulating pump, respectively; and operating the circulating pump to circulate a fluid through the blood vessel mimic. 1. A method for culturing a blood vessel mimic , which comprises the steps of:printing a blood vessel mimic, such that a solution in which calcium ions are dissolved forms a core layer; a tubular first layer that encompasses the core layer is formed using a first bioink in which vascular endothelial cells and alginate are mixed with a decellularized extracellular matrix isolated from a blood vessel tissue; and a tubular second layer that encompasses the first layer is formed using a second bioink, in which smooth muscle cells and alginate are mixed with a decellularized extracellular matrix isolated from a blood vessel tissue;connecting, to both ends of the blood vessel mimic, tubes connected to a circulating pump, respectively; andoperating the circulating pump to circulate a fluid through the blood vessel mimic through the core layer.2. The method of claim 1 , wherein claim 1 , in the printing a blood vessel mimic claim 1 , the first layer and the second layer are crosslinked by reacting with the calcium ions.3. The method of claim 1 , wherein the method further comprises controlling the perfusion pressure of the fluid by controlling the circulating pump claim 1 , such that the first layer is cultured with vascular endothelial cells and the second layer is cultured with smooth muscle cells claim 1 , andthe vascular endothelial cells are arranged such that the flow direction of the fluid becomes the long axis, and the smooth muscle cells are arranged such that a direction ...

MICROFLUIDIC THREE-DIMENSIONAL OSTEOCYTE NETWORK RECONSTRUCTED WITH MICROBEADS AS SCAFFOLD

A bed of microbeads is used as a foundation for reconstructing a three-dimensional osteocyte network by culturing osteocytes within the bed. The osteocytes are cultured such that they form a network among the microbeads that is capable of simulating the osteocyte network of natural bone. The osteocytes are cultured in a microfluidic device adapted for the purpose. 1. A method of culturing osteocytes in a microfluidic chamber , comprising the steps of:mixing a plurality of cells with a plurality of microbeads, thereby forming a mixture including cells and microbeads, the cells including one or both of a plurality of osteocytes and a plurality of pre-osteocytes;adding a portion of the mixture to a microfluidic chamber, thereby forming a bed of microbeads with cells distributed among the microbeads;allowing the cells of the bed to attach to the microbeads of the bed; andperfusing the bed with a culture medium, whereby osteocytes of the bed remain as osteocytes and pre-osteocytes of the bed develop into osteocytes.2. The method of claim 1 , wherein said perfusing step is performed such that one or both of the osteocytes of the bed and the pre-osteocytes of the bed form a network of osteocytes among the microbeads of the bed.3. The method of claim 1 , wherein the microbeads of the bed have diameters such that the bed has an interstitial space between adjacent ones of the microbeads of the bed claim 1 , the interstitial space having a size such that only one osteocyte or pre-osteocyte occupies the interstitial space.4. The method of claim 1 , wherein the cells are selected from a species of animal claim 1 , and the microbeads have diameters that are approximately the same size as a typical distance between osteocytes in a living animal of the species.5. The method of claim 1 , wherein the plurality of microbeads includes biphasic calcium phosphate.6. The method of claim 2 , wherein the culture medium includes a biologically-active substance claim 2 , and said method ...

Polymeric Carriers and Methods

Provided are methods of controlling disassociation of cells from a carrier, compositions, and methods of collecting cells. The methods of controlling disassociation of cells from a carrier may include contacting a polymeric carrier with one or more digesting agents to disassociate at least a portion of a plurality of cells from the polymeric carrier. The polymeric carrier may be crosslinked with a crosslinker including at least one of a redox sensitive moiety, a UV light sensitive moiety, a pH sensitive moiety, and a temperature sensitive moiety. 1. A method of controlling disassociation of cells from a carrier , the method comprising:providing a polymeric carrier and a plurality of cells adhered to the polymeric carrier; andcontacting the polymeric carrier with one or more digesting agents to disassociate at least a portion of the plurality of cells from the polymeric carrier;wherein the polymeric carrier is crosslinked with a crosslinker comprising at least one redox sensitive moiety.2. The method of claim 1 , wherein the at least one redox sensitive moiety comprises a disulfide bond.13. A method of controlling disassociation of cells from a carrier claim 1 , the method comprising:providing a polymeric carrier and a plurality of cells adhered to the polymeric carrier; andcontacting the polymeric carrier with one or more digesting agents to disassociate at least a portion of the plurality of cells from the polymeric carrier;wherein the polymeric carrier is crosslinked with a crosslinker comprising at least one of a UV light sensitive moiety, a pH sensitive moiety, and a temperature sensitive moiety.14. The method of claim 13 , wherein the crosslinker comprises the UV light sensitive moiety claim 13 , and the UV light sensitive moiety is a photoreversibly dimerizable moiety or a photocleavable moiety.19. The method of claim 14 , wherein the crosslinker comprises the photocleavable moiety claim 14 , and the photocleavable moiety comprises an o-nitrobezene based ...

OSTEOPOROSIS MODEL COMPRISING CALCIUM PHOSPHATE HYDROGEL COMPOSITION AND USE THEREOF

Provided is a method of preparing a hydrogel composition including a uniform content of calcium phosphate, wherein a hydrogel composition prepared by the method has a uniform content of calcium phosphate, and thus may be used to quantify phosphates contained in the hydrogel composition. Provided is an in-vitro 3D osteoporosis model including a calcium phosphate hydrogel composition, wherein osteoblasts and osteoclasts may be three-dimensionally co-cultured inside a biogel, such that the osteoporosis model may be fabricated according to an intended use or clinical stage. Further, the model contains a calcium phosphate hydrogel with a uniform content of phosphate and thus enables quantification of calcium phosphate through measurement of phosphates, and therefore, the model may be used to screen candidate compounds for an osteoporosis drug and may effectively predict therapeutic effects of the drug on osteoporosis. 1. A method comprising:preparing a first composition by dissolving chitosan in a gelatin solution; preparing a second composition by dispersing calcium phosphate in a calcium chloride solution; andmixing the first composition with the second composition, to thereby prepare a calcium phosphate hydrogel composition.2. The method of preparing a hydrogel composition of claim 1 , further comprising: preparing a hydrogel bead composition by adding dropwise the hydrogel composition into an alkaline solution.3. The method of preparing a hydrogel composition of claim 1 , wherein the chitosan is included in an amount of 0.1 wt % to 10 wt % in the first composition.4. The method of preparing a hydrogel of claim 1 , wherein the calcium phosphate is included in an amount of 0.1 wt % to 10 wt % in the second composition.5. The method of preparing a hydrogel of claim 1 , wherein the preparation of the second composition further comprises conducting sonification subsequent to dispersal of the calcium phosphate in the calcium chloride solution.6. The method of preparing a ...

METHODS FOR CONTROLLED INDUCTION OF 3D CYLINDRICAL NEUROEPITHELIAL TUBES

Described herein are methods, compositions, and kits for forming engineered in vitro biomimetic, three-dimensional, tubular organoid structures by directed differentiation of human pluripotent stem cells within tubular channels formed in a hydrogel. 1. A method of preparing an engineered biomimetic 3D organoid in vitro , the method comprising:(a) providing a hydrogel having a channel therein, the channel having a first end and a second end;(b) sealing one or more of the first and second ends of the channel;(c) seeding the channel with human pluripotent stem cells (hPSCs),(d) contacting the hydrogel comprising the hPSC-seeded channel to an alginate solution, and then contacting the alginate-contacted hydrogel to a divalent cation solution, thereby producing a hPSC-seeded channel encapsulated in crosslinked hydrogel, and(e) culturing the encapsulated hPSC-seeded channel in a culture medium for about four (4) to about sixteen (16) days for the hPSCs within the channel to differentiate into neuroepithelial cells, whereby a biomimetic 3D organoid comprising polarized neuroepithelial cells and having microscale cellular organization similar to that of an in vivo developing human neural tube is obtained.2. The method of claim 1 , wherein the hydrogel is an alginate hydrogel.3. The method of claim 1 , wherein the divalent cation solution is a calcium chloride (CaCl) solution.4. The method of claim 1 , wherein sealing comprises contacting the first end or the second end of the channel to an alginate solution and contacting the alginate-contacted end to a divalent cation solution claim 1 , thereby producing a channel comprising a sealed end and an unsealed end.5. The method of claim 4 , wherein seeding the channel comprises injecting a hPSC suspension having a cell density of about 500 claim 4 ,000 cells/μl or less into the unsealed end.6. The method of claim 1 , wherein sealing comprises contacting the first end and the second end of the channel into an alginate solution and ...

STEM CELL CARRIER AND METHOD FOR BONE REGENERATION WITH 3D CUSTOMIZED CAD/CAM USING THE CARRIER

A stem cell carrier includes a plurality of single units. The plurality of single units are formed three-dimensionally interconnected to each other to form a cubic structure. The plurality of single units are made of a porous material. Each single unit has three-dimensionally formed through holes crossing each other, and each through hole has 400 to 500 micrometers in diameter. The through holes of the plurality of single units are interconnected to each other to form a plurality of channels which are formed from one side of the cubic structure to the other side. 1: A stem cell carrier comprising a plurality of single units , the plurality of single units formed three-dimensionally interconnected to each other to form a cubic structure , the plurality of single units made of a porous material , wherein each single unit has three-dimensionally formed through holes crossing each other , each through hole having 400 to 500 micrometers in diameter , and the through holes of the plurality of single units are interconnected to each other to form a plurality of channels which are formed from one side of the cubic structure to the other side.2: The stem cell carrier of claim 1 , wherein the porous material is selected from the group consisting of hydroxyapatite (HA) claim 1 , β-TCP (tricalcium phosphate) claim 1 , and mixtures thereof.3: A method for bone regeneration with 3D customized computer-aided design and computer-aided manufacturing (CAD/CAM) claim 1 , the method comprising:imaging a bone defect using CT and MRI;sending the image information of the bone defect to a CAD/CAM;{'claim-ref': {'@idref': 'CLM-00001', 'claim 1'}, 'forming a scaffold by shaping the stem cell carrier of to have the bone defect;'}storing stem cells in the formed scaffold; andfixing the scaffold storing the stem cells with a fixing device comprised of a biocompatible material.4: The stem cell carrier of claim 1 , wherein each single unit has a first through hole claim 1 , a second through hole ...

Cell transfer agent

The present invention is a cell transfer agent comprising a composite particle coated by a sugar chain polymer wherein the composite particle consisting of an apatite comprising phosphate, carbonic acid, and calcium.

Compositions for treatment of osteochondral disorders

The application provides biocompatible carriers comprising bone forming and/or cartilage forming cells and methods for making them. The application further provides pharmaceutical compositions comprising said ATMPs and method of treatments using said ATMPs. The application further relates to said ATMPS for use in the treatment of bone disorders, cartilage disorders and joint disorders. The current invention further relates to method of treatments of bone disorders, cartilage disorders and joint disorders.

Compositions Comprising Citrate and Applications Thereof

In one aspect, methods of promoting bone growth are described herein. In some embodiments, a method of promoting bone growth described herein comprises promoting cell differentiation or phenotype progression in a population of bone cells by providing a citrate-presenting composition to the population of bone cells. In some embodiments, the citrate-presenting composition is provided to the bone cells at a first stage of cell development selected to obtain a first cell differentiation or phenotype progression. Additionally, in some cases, a second citrate-presenting composition is further provided to the bone cells at a second stage of cell development selected to obtain a second cell differentiation or phenotype progression. 116-. (canceled)17. A cell culture medium comprising a citrate-presenting composition , wherein the citrate-presenting composition is effective to provide a free citrate and/or free citric acid at a concentration between about 20 μM and about 2000 μM based on the volume of the composition , and wherein the cell culture medium is an aqueous mixture or a solution.18. The cell culture medium of claim 17 , wherein the citrate-presenting composition comprises citric acid claim 17 , a citrate salt claim 17 , an ester of citric acid claim 17 , or a combination thereof.20. The cell culture medium of claim 19 , wherein M is Na or K.22. The cell culture medium of claim 17 , further comprising a population of bone cells.23. The cell culture medium of claim 22 , wherein the population of bone cells comprises osteoblast cells claim 22 , bone marrow cells claim 22 , or stem cells.24. The cell culture medium of claim 23 , wherein the stem cells comprise multipotent claim 23 , oligopotent or unipotent stem cells.25. The cell culture medium of claim 23 , wherein the stem cells comprise mesenchymal stem cells (MSCs).26. The cell culture medium of claim 17 , further comprising Minimum Essential Medium alpha (αMEM) claim 17 , Eagle's minimal essential medium (EMEM) ...

DIGESTIBLE SUBSTRATES FOR CELL CULTURE

A cell culture article is provided. The cell culture substrate includes a polygalacturonic acid compound selected from at least one of: pectic acid or salts thereof, and partially esterified pectic acid having a degree of esterification from 1 to 40 mol % or salts thereof. The polygalacturonic acid compound is crosslinked with a divalent cation and the divalent cation concentration ranges from 0.5 to 2 g/1 of the substrate. 1. A cell culture article , comprising:a substrate comprising a polygalacturonic acid compound selected from at least one of:pectic acid or salts thereof, andpartially esterified pectic acid having a degree of esterification from 1 to 40 mol % or salts thereof,wherein the polygalacturonic acid compound is crosslinked with a divalent cation and the divalent cation concentration ranges from 0.5 to 2 g/l of the substrate.2. The article of claim 1 , wherein the substrate is spherical or substantially spherical.3. The article of claim 2 , wherein the substrate comprises a diameter of 10 to 500 micrometers.4. The article of claim 1 , wherein a plurality of the cell culture articles comprise a coefficient of variation of less than 20%.5. The article of claim 1 , wherein a plurality of the cell culture articles comprise a coefficient of variation of less than 10%.6. The article of claim 2 , wherein a plurality of the cell culture articles comprise size spread Δd5-d95 of less than 25 micrometers claim 2 , wherein d5 is a diameter that is larger than the diameters of 5% of the plurality of the cell culture articles claim 2 , wherein d95 is a diameter that is larger than the diameters of 95% of the plurality of the cell culture articles claim 2 , and wherein Δd5-d95 is the difference between d95 and d5.7. The article of claim 2 , wherein a plurality of the cell culture articles comprise size spread Δd10-d90 of less than 20 micrometers claim 2 , wherein d10 is a diameter that is larger than the diameters of 10% of the plurality of the cell culture articles ...

Apparatus and process for the preparation of a biomimetic tissue prosthesis of the tympanic membrane

The present invention refers to a process, and to the related apparatus, for the in vitro preparation of a biomimetic tissue prostheses of the tympanic membrane from mesenchymal stem cells; such prostheses are used for repairing or reconstructing the injured tympanic membrane in patients needing it, for example as a consequence of various trauma or pathologies.

Algae Beads

Algae-containing beads comprising a microorganism such as unicellular microalgae, an insoluble carbon source such as char, water, and a crosslinked organic matrix. The beads can further contain clay, such as kaolin. 1. An algal bead comprising unicellular microalgae , char , and a crosslinked organic matrix.2. The bead of claim 1 , further comprising a clay.3. The bead of claim 1 , wherein the organic matrix before crosslinking comprises a plurality of hydroxyl groups that react with a divalent cation to crosslink the matrix.4. The bead of claim 3 , wherein the organic matrix comprises a water soluble polysaccharide selected from the group consisting of alginate salts claim 3 , galactomannan claim 3 , gellan gum claim 3 , carrageenan claim 3 , agarose claim 3 , and mixtures thereof.5. The bead of claim 2 , wherein the clay comprises kaolin.6. The bead of claim 1 , wherein the char comprises a biochar.7. The bead of claim 6 , wherein the biochar is produced from algal biomass or rice hulls.8. The bead of claim 1 , further comprising soluble salts of cations selected from Na+ claim 1 , K+ claim 1 , Mg+2 claim 1 , Ca+2 claim 1 , Fe+3 claim 1 , Mn+2 claim 1 , Zn+2 claim 1 , Cu+2 claim 1 , and Co+2.9. The bead of claim 8 , comprising soluble salts of one or more anions selected from nitrates claim 8 , phosphates claim 8 , hydrogen phosphates claim 8 , dihydrogen phosphates claim 8 , sulfates claim 8 , chlorides claim 8 , EDTA claim 8 , carbonates claim 8 , bicarbonates claim 8 , and molybdates.10Chlorella, Spirulina, Dunaliella, Haematococcus, Crypthecodinium, Schizochytrium, Scenedesmus, Aphanizomenon, Arthrospira, Odontella, Isochrysis, Nannochloropsis, Tetraselmis, Phaeodactylum, Porphyridium,. The bead of claim 1 , wherein the microalgae are selected from the group consisting of and combinations thereof.11. The bead of claim 3 , wherein the divalent ion is selected from Be+2 claim 3 , Mg+2 claim 3 , Ca+2 claim 3 , Sr+2 claim 3 , and Ba+2.12. A method of making a bead ...

NOVEL MICROCARRIER BEADS

The invention relates to a novel microcarrier bead; a method for producing same; a therapeutic comprising said microcarrier bead and attached thereto or grown thereon at least one selected cell or tissue type; a method for making said therapeutic; and a method of treatment involving the use of said microcarrier bead or said therapeutic. 1. A microcarrier bead made from apatite and characterised by one or more , including any combination , of the following features:a) micrometre-sized;b) a regular porous structure;c) rough surface;d) substantially spherical;e) osteo-conductivity;f) chemical similarity to the mineral phase of natural bone; andg) high thermal stability permitting them to be easily sterilized.2. A microcarrier bead according to wherein said apatite is selected form the group comprising: hydroxyapatite claim 1 , silicon-substituted apatite claim 1 , silver-substituted apatite claim 1 , magnesium-substituted apatite and a stoichiometric apatite which is a synthetic apatite with a Ca/P atomic ratio that approaches 1.67.3. A microcarrier bead according to wherein said apatite is phase-pure.4. A microcarrier bead according to wherein said beads are between 100-800 μm diameter claim 1 , or 200-600 μm diameter claim 1 , or 400-500 μm diameter.5. A microcarrier bead according to wherein said beads have a regular pore size as observed by Scanning Electron Microscopy.6. A microcarrier bead according to wherein said beads can withstand temperatures up to 1500° C. for up to 10 hours.7. A microcarrier bead according to wherein said beads have osteogenic potency.8. A plurality of microcarrier beads according to any one of .9. A method for making microcarrier beads comprising:a) mixing apatite and alginate in a solution and allowing them to disperse to form a suspension;b) extruding said suspension drop-wise through a droplet device;{'sub': '2', 'c) exposing said extruded droplets to calcium chloride (CaCl) solution;'}{'sub': '2', 'd) washing said beads to remove said ...

Compositions Comprising Citrate and Applications Thereof

In one aspect, methods of promoting bone growth are described herein. In some embodiments, a method of promoting bone growth described herein comprises promoting cell differentiation or phenotype progression in a population of bone cells by providing a citrate-presenting composition to the population of bone cells. In some embodiments, the citrate-presenting composition is provided to the bone cells at a first stage of cell development selected to obtain a first cell differentiation or phenotype progression. Additionally, in some cases, a second citrate-presenting composition is further provided to the bone cells at a second stage of cell development selected to obtain a second cell differentiation or phenotype progression. 1. A method for promoting bone growth , said method comprising providing to a subject in need thereof free citric acid or citrate at a concentration of between about 20 μM and about 2000 μM , thereby promoting bone growth in said subject.2. The method of claim 1 , wherein providing to the subject free citric acid or citrate comprises administering to the subject a citrate-presenting composition claim 1 , wherein free citric acid or citrate is released from said citrate-presenting composition.3. The method of claim 2 , wherein said citrate-presenting composition comprises a polymer or oligomer comprising a citrate moiety.5. The method of claim 4 , wherein said polymer or oligomer comprises the reaction product of (i) citric acid claim 4 , or an ester of citric acid with (ii) a polyol.6. The method of claim 5 , wherein said ester of citric acid is triethyl citrate.7. The method of claim 5 , wherein said polyol is a diol.9. The method of claim 8 , wherein R claim 8 , R claim 8 , and Rare —H claim 8 , Ris —OH claim 8 , and Ris —H.11. The method of claim 10 , wherein R claim 10 , R claim 10 , and Rare —H claim 10 , or —CHCH claim 10 , Ris —OH claim 10 , Ris —H claim 10 , n is 2 to 6 claim 10 , m is 2 to 8 claim 10 , and p is 2 to 6.13. The method of ...

IN VITRO MODELLING OF HAEMATOPOIETIC STEM CELL MEDULLARY NESTS: A TOOL FOR STUDYING THE REGULATION OF HAEMATOPOIESIS, EVALUATING THE NESTING POTENTIAL OF A HAEMATOPOIETIC GRAFT AND TESTING THE PHARMACOTOXICOLOGY OF MEDICAMENTS

The present invention relates to a culture support for cultivating hematopoietic stem cells (HSCs) and/or hematopoietic progenitors (HPs), comprising a calcium biomaterial, osteoclasts, endothelial cells and mesenchymatous stem cells (MSCs) and/or osteoblasts and/or adipocytes. The present invention also relates to a method for preparing such a culture support, and an in vitro HSC and/or HP cultivation method. The use of such a culture support for studying cellular mechanisms involved in hematopoiesis and/or differentiation of HSC/HPs and/or for studying the efficacy and/or the toxicity of a medicament candidate is also described. 1. A culture support of hematopoietic stem cells (HSCs) and/or hematopoietic progenitors (HPs) , comprising:a. a calcium biomaterial;b. osteoclasts;c. endothelial cells; andd. mesenchymatous stem cells (MSCs) and/or osteoblasts and/or adipocytes.2. The culture support according to claim 1 , wherein said calcium biomaterial comprises hydroxyapatite (HA) and tricalcium phosphate (TCP).3. The culture support according to claim 1 , wherein said calcium biomaterial is two dimensional or three dimensional.4. The culture support according to claim 1 , wherein said calcium biomaterial is selected from the group consisting of the biomaterials B2D and B3D (BD Biocoat Osteologic Bone Cell Culture System) and Calciresorb 35 (Ceraver claim 1 , France).5. The culture support according to claim 1 , which further comprises one or more component(s) of the extracellular matrix (EMC).6. The culture support according to claim 1 , which further comprises a biological glue.7. A method for preparing a culture support as defined in claim 1 , comprising the steps consisting of grafting at least one calcium biomaterial with osteoclasts claim 1 , endothelial cells and mesenchymatous stem cells (MSCs) and/or osteoblasts and/or adipocytes and optionally one or more component(s) of the ECM.8. An in vitro method for culturing hematopoietic stem cells (HSCs) and/or ...

MODULAR SYNTHETIC TISSUE-GRAFT SCAFFOLD

A modular synthetic tissue-graft scaffold () includes one or more nominally identical scaffold cages () configured to facilitate regrowth of tissue of an organism in and around the scaffold cages. Each scaffold cage comprises a volumetric enclosure () bounded by a perforated wall structure (). A recess () formed at one end of the volumetric enclosure defines an inner stepped coupling surface. An annular raised portion () positioned at the other end of the volumetric enclosure forms an outwardly projecting stepped seating surface sized to form a complementary matable surface to the inner stepped coupling surface for whenever an inner stepped coupling surface of another one of the cages is placed on the outer stepped seating surface of the scaffold cage. Corridors () extending through the perforated wall structure and communicating with passageways () within the volumetric enclosure enable migration of material within and out of the scaffold cage. 1. In a modular , synthetic tissue-graft scaffold including a set of one or more nominally identical scaffold cages that are configured to facilitate regrowth of tissue of an organism in and around the scaffold cages , each scaffold cage in the set comprising:a volumetric enclosure bounded by a perforated wall structure and having interior and exterior surfaces and first and second opposite ends, the volumetric enclosure defining a central longitudinal axis that extends through the first and second opposite ends, the interior surface defining a boundary of an interior chamber of the volumetric enclosure, and the interior and exterior surfaces defining between them a thickness of the perforated wall structure;a perforated platform set within the volumetric enclosure and in transverse relation to the central longitudinal axis forms a recess at the first end of the volumetric enclosure, the recess defining an inner stepped coupling surface bounded by the interior surface of the perforated wall structure, the perforated platform ...

Cell culture substratum, method for producing cell-containing material, method for producing cell culture substratum, method for observing cells, and cell culture substratum maintenance fluid

The purpose of the present invention is to provide a cell culture substratum which has excellent resistance to liquid culture media and low cytotoxicity, can achieve a high cell adhesion ratio and a high viability of cultured cells, has excellent thermal stability, and is less likely to absorbs ultraviolet ray. A cell culture substratum which is provided with a substrate made from an inorganic material and has multiple concavo-convex structures on a culturing surface thereof, wherein, when the concavo-convex structures are measured with an atomic force microscope in accordance with JISB0601 and JISR1683 (measured area: a 1 μm-square, cut-off value of a low-pass contour curve filter: 1 nm, cut-off value of a high-pass contour curve filter: 170 nm), the average of the lengths of contour curve elements of the concavo-convex structures is 1 to 170 nm as measured in at least one direction (when a curve showing long-wavelength components that are blocked by the high-pass contour curve filter is converted to a straight line by the least square method, the average line is a line that is parallel with the straight line and indicates a height cumulative relative frequency distribution in the contour curve of 50%).

DISSOLVABLE FOAM SCAFFOLDS FOR CELL CULTURE AND METHODS OF MAKING THE SAME

A dissolvable foam scaffold for cell culture is provided herein. The dissolvable foam scaffold includes an ionotropically crosslinked polygalacturonic acid compound selected from at least one of: pectic acid; partially esterified pectic acid, partially amidated pectic acid and salts thereof, and at least one first water-soluble polymer having surface activity.

Process for Making Mineralized Mycelium Scaffolding and Product Made Thereby

The process of making a mineralized mycelium scaffolding requires obtaining a scaffold of fungal biopolymer having a network of interconnected mycelia cells, functionalizing the biopolymer to create precursor sites and thereafter mineralizing the scaffold with one of silicates, apatites and carbonates. The mineralized mycelium scaffolding may be used for medical applications in place of mineralized collagen membranes and collagen/hydroxyapatite composite scaffolds. 1. A structure comprisinga scaffold of fungal biopolymer of predetermined form characterized in being formed of a network of interconnected mycelia cells; anda coating of one of hydroxyapatite, calcite and silicate on at least some of said cells within said network.2. A structure as set forth in wherein said predetermined form is a flat panel shape with a thickness of 2.5 cm.3. A process of making a mineralized mycelium scaffolding comprising the steps ofobtaining a scaffold of fungal biopolymer having a network of interconnected mycelia cells; andthereafter mineralizing said scaffold.4. A process as set forth in wherein said fungal biopolymer of said scaffold has a chitin/chitosan backbone and said process further comprises a step of functionalizing said scaffold by removing a —OH moiety therefrom and replacing said moiety with a phosphate group to increase an affinity for cations.5. A process as set forth in wherein said step of mineralizing said scaffold with at least one of silicates claim 4 , apatites claim 4 , and carbonates.6. A process as set forth in further comprising the steps ofplacing said functionalized scaffold in a saturated calcium hydroxide solution for a period of time sufficient to create calcium phosphate precursor sites on said mycelia cells of said scaffold;thereafter suspending said scaffold in a solution with ion concentrations 1.5 times that of the human body and a pH of 7.25 for a period of time sufficient to precipitate a hydroxyapatite coating on said cells of said scaffold; ...

Compositions Comprising Citrate and Applications Thereof

In one aspect, methods of promoting bone growth are described herein. In some embodiments, a method of promoting bone growth described herein comprises promoting cell differentiation or phenotype progression in a population of bone cells by providing a citrate-presenting composition to the population of bone cells. In some embodiments, the citrate-presenting composition is provided to the bone cells at a first stage of cell development selected to obtain a first cell differentiation or phenotype progression. Additionally, in some cases, a second citrate-presenting composition is further provided to the bone cells at a second stage of cell development selected to obtain a second cell differentiation or phenotype progression. 1a first polymer or oligomer having the structure of Formula (I):. A composition comprising:wherein{'sub': '1', 'img': [{'@id': 'CUSTOM-CHARACTER-00006', '@he': '2.46mm', '@wi': '8.47mm', '@file': 'US20180369277A1-20181227-P00001.TIF', '@alt': 'custom-character', '@img-content': 'character', '@img-format': 'tif'}, {'@id': 'CUSTOM-CHARACTER-00007', '@he': '2.46mm', '@wi': '8.47mm', '@file': 'US20180369277A1-20181227-P00001.TIF', '@alt': 'custom-character', '@img-content': 'character', '@img-format': 'tif'}], 'Ris —H, —OC(O)NH, or ;'}{'img': {'@id': 'CUSTOM-CHARACTER-00008', '@he': '2.46mm', '@wi': '8.47mm', '@file': 'US20180369277A1-20181227-P00001.TIF', '@alt': 'custom-character', '@img-content': 'character', '@img-format': 'tif'}, 'represents an additional chain of repeating units having the structure of Formula (I); and'}m, n, and p are 8; and 'a second polymer or oligomer having the structure of Formula (II):', 'q is 6; and'}wherein{'sub': '2', 'img': {'@id': 'CUSTOM-CHARACTER-00009', '@he': '2.46mm', '@wi': '8.47mm', '@file': 'US20180369277A1-20181227-P00001.TIF', '@alt': 'custom-character', '@img-content': 'character', '@img-format': 'tif'}, 'Ris —H or ;'}{'img': {'@id': 'CUSTOM-CHARACTER-00010', '@he': '2.46mm', '@wi': '8.47mm', '@file': ' ...

Bioactive Soft Tissue Implant and Methods of Manufacture and Use Thereof

A bioactive filamentary structure includes a sheath coated with a mixture of synthetic bone graft particles and a polymer solution forming a scaffold structure. In forming such a structure, synthetic bone graft particles and a polymer solution are applied around a filamentary structure. A polymer is precipitated from the polymer solution such that the synthetic bone graft particles and the polymer coat the filamentary structure and the polymer is adhered to the synthetic bone graft particles to retain the graft particles. 1. A method of forming a bioactive filamentary structure , comprising the steps of:applying a polymer solution around a filamentary structure;after the polymer solution applying step, applying synthetic bone graft particles to the polymer solution and around the filamentary structure; andafter the synthetic bone graft particles applying step, precipitating a polymer from the polymer solution such that the synthetic bone graft particles and the polymer coat the filamentary structure.2. The method of claim 1 , wherein the synthetic bone graft particles are applied around the filamentary structure by placing the coated filamentary structure into a container of synthetic bone graft particles and then removing the filamentary structure from the container.3. The method of claim 2 , wherein the container of the synthetic bone graft particles is shaken during placement of the coated filamentary structure into the container.4. The method of claim 1 , wherein the synthetic bone graft particles include either one or both of calcium phosphate and a bioactive additive.5. The method of claim 1 , wherein the polymer solution includes at least one solvent selected from the group consisting of glacial acetic acid (GAA) claim 1 , acetic acid claim 1 , anisole claim 1 , chloroform claim 1 , methylene chloride claim 1 , acetylchloride claim 1 , 2 claim 1 ,2 claim 1 ,2 trifluoroethanol claim 1 , trifluoroacetic acid claim 1 , 1 claim 1 ,2-Dochloroethane claim 1 , and ...

A kind of bone biological function analyzes porous cell culture plate

本发明公开了一种骨生物功能分析多孔细胞培养板，旨在于提供一种磷酸钙复合物桥连均匀，表面结构完整，保持有理想的骨仿生合成表面结构的细胞培养板；其技术方案通过下述步骤制备：1400～1600℃高温煅烧无水磷酸氢钙和碳酸钙混合生成TTCP，研磨搅拌过筛获得直径1～80μm的TTCP颗粒；研磨搅拌过筛获得直径0.4～3.0μm的DCPA颗粒；按摩尔质量比1～3:3～1混合TTCP和DCPA制备CPC粉末，以双蒸水作为液相制得液相CPC；再加入双蒸水，超声搅拌得到CPC原液溶度1mg～5mg/ml的CPC混悬液。

Porous composite materials

Methods and compositions are described that provide three dimensional porous matrices as structural templates for cells. The porous matrices of the present invention have desirable mechanical properties suitable to a variety of applications, including platforms for in vitro cell cultivation, implants for tissue and organ engineering, and materials suitable for chromatography and filtration.

Human mesenchymal stem cells

Isolated human mesenchymal stem cells which can differentiate into more than one tissue type (e.g. bone, cartilage, muscle or marrow stroma), a method for isolating, purifying, and culturally expanding human mesenchymal stem cells (i.e. "mesenchymal stem cells" or "hMSCs"), and characterization of and uses, particularly research reagent, diagnostic and therapeutic uses for such cells. The stem cells can be culture expanded without differentiating. Monoclonal antibodies specific for human mesenchymal stem cells and the monoclonal hybridoma cell lines (ATCC nos. 10743-10745) that synthesize and secrete these monospecific antibodies, and uses of the monoclonal antibodies for diagnostic and/or therapeutic purposes.

Implant for articular cartilage repair

An implant for articular cartilage repair includes (1) a three-dimensional body formed of cancellous bone having a demineralized section that contains bone morphogenetic proteins (BMP's) that are released by the demineralization but retained in the body, and (2) a cartilage layer formed on a surface of the demineralized section. The cartilage layer is formed by a method including the steps of (a) isolating chondrocytes from articular cartilage of a donor; (b) cultivating the isolated chondrocytes in a medium; (c) suspending the cultivated chondrocytes in agarose; (d) adding the cultivated chondrocytes to the demineralized section of the body, whereby the cultivated chondrocytes are stimulated by the BMP's retained in the body; and (e) incubating the cultivated chondrocytes to form a plurality of layers of chondrocytes on the demineralized section, wherein the plurality of layers of chondrocytes forms the cartilage layer.

Biodegradable synthetic polymeric fibrous matrix containing chondrocyte for in vivo production of a cartilaginous structure

Methods and artificial matrices for the growth and implantation of cartilaginous structures and surfaces and bone are disclosed. In the preferred embodiments, chondrocytes are grown on biodegradable, biocompatible fibrous polymeric matrices. Optionally, the cells are proliferated in vitro until an adequate cell volume and density has developed for the cells to survive and proliferate in vivo. One advantage of the matrices is that they can be cast or molded into a desired shape, on an individual basis, so that the final product closely resembles a patient's own ear or nose. Alternatively, flexible matrices can be used which can be manipulated at the time of implantation, as in a joint, followed by remodeling through cell growth and proliferation in vivo. The cultured cells can also be maintained on the matrix in a nutrient media for production of bioactive molecules such as angiogenesis inhibiting factor. Examples are provided showing the growth of hyaline cartilage for joint relinings, the growth of elastic cartilage for plastic or reconstructive replacement of cartilage structures, and repair of large bone defects.

Apatite microcarrier and preparation method and application thereof

本发明属于生物材料技术领域，公开了一种磷灰石微载体及其制备方法和应用。该磷灰石微载体的耐热温度为1000‑1350℃；磷灰石微载体的主要成分选自羟基磷灰石、卤素磷灰石、磷酸三钙、磷酸四钙或经离子置换的磷灰石中的至少一种。本发明还提供一种磷灰石微载体的制备方法。该磷灰石微载体在1150℃及以上的高温下，仍然具有良好的热稳定性，可充分灭菌。该磷灰石微载体不残留有机溶剂，对细胞无毒性，具有良好的生物相容性，有利于该磷灰石微载体在医疗领域的应用。

Methods and compositions for treatment of bone defects with placental cell populations

Provided herein are methods of using adherent placental stem cells and placental stem cell populations, and methods of culturing, proliferating and expanding the same. Also provided herein are methods of differentiating the placental stem cells. Further provided herein are methods of using the placental stem cells to formulate implantable or injectable compositions suitable for administration to a subject. Still further provided herein are provides methods for treating bone defects with stem cells and compositions comprising stem cells.

Method for enhancing the implantation and differentiation of marrow-derived mesenchymal cells

The present invention is directed to a method and device for enhancing the implantation and differentiation of marrow-derived mesenchymal cells (i.e. mesenchymal stem cells). The method and device of the invention are an effective means for treating skeletal and other connective tissue disorders.

Method for treating connective tissue disorders

The present invention is directed to various processes and devices for utilizing isolated and culturally expanded marrow-derived mesenchymal cells (i.e. mesenchymal stem cells) for treating skeletal and other connective tissue disorders.

Enhancing bone marrow engraftment using MSCS

Method and preparations for enhancing bone marrow engraftment in an individual by administering (i) a culturally expanded mesenchymal stem cell preparation and (ii) a bone marrow graft. The mesenchymal stem cells are administered in an mount effective to promote engraftment of the bone marrow.

Connective tissue regeneration using human mesenchymal stem cell preparations

Isolated human mesenchymal stem cells, a method for isolating, purifying, and culturally expanding human mesenchymal stem cells (i.e. mesenchymal stem cells or "MSCs"), and characterization of (including by cytokine expression profiling) and uses, particularly research reagent, diagnostic and therapeutic uses for such cells. The stem cells can be culture expanded without differentiating. Monoclonal antibodies specific for human mesenchymal stem cells and the monoclonal hybridoma cell lines (ATCC nos. 10743-10745) that synthesize and secrete these monospecific antibodies, and uses of the monoclonal antibodies for diagnostic and/or therapeutic purposes.

Monoclonal antibodies for human mesenchymal stem cells

The invention relates to isolated human mesenchymal stem cells, a method for isolating, purifying, and culturally expanding human mesenchymal stem cells (i.e. mesenchymal stem cells or "MSCs"), and characterization of (including by cytokine expression profiling) and uses, particularly research reagent, diagnostic and therapeutic uses for such cells. The stem cells can be culture expanded without differentiating. Further disclosed are monoclonal antibodies specific for human mesenchymal stem cells and the monoclonal hybridoma cell lines (ATCC nos. 10743-10745) that synthesize and secrete these monospecific antibodies, and uses of the monoclonal antibodies for diagnostic and/or therapeutic purposes.

Regulation of hematopoietic stem cell differentiation by the use of human mesenchymal stem cells

The invention relates to the induction of hematopoietic stem cells to differentiate into osteoclasts by culturing hematopoietic stem cells with human mesenchymal stem cells, and, in a preferred embodiment, using no exogenous cytokines. Differentiation of the mesenchymal stem cells into osteoblasts inhibited the differentiation of hematopoietic stem cells into osteoclasts. In addition, hematopoietic stem cells can be genetically engineered to carry genes of interest particularly for the expression of physiologically active proteins. In the presence of mesenchymal stem cells, the transduced cells carry the new genetic material and express gene products that can be used to modulate bone resorption.

Methods and compositions for treatment of bone defects with placental cell populations

Provided herein are placental stem cells for use in a method for treatment of a bone defect in an individual, wherein said bone defect is an osteolytic lesion associated with a bone fracture, or a spine in need of fusion, wherein said placental stem cells are CD200+, HLA-G+ placental stem cells or CD200+, OCT-4+ placental stem cells.

Methods and compositions for treatment of bone defects with placental cell populations

Provided herein are methods of using adherent placental stem cells and placental stem cell populations, and methods of culturing, proliferating and expanding the same. Also provided herein are methods of differentiating the placental stem cells. Further provided herein are methods of using the placental stem cells to formulate implantable or injectable compositions suitable for administration to a subject. Still further provided herein are provides methods for treating bone defects with stem cells and compositions comprising stem cells.

Methods and compositions for treatment of bone defects with placental cell populations

Provided herein are methods of using adherent placental stem cells and placental stem cell populations, and methods of culturing, proliferating and expanding the same. Also provided herein are methods of differentiating the placental stem cells. Further provided herein are methods of using the placental stem cells to formulate implantable or injectable compositions suitable for administration to a subject. Still further provided herein are provides methods for treating bone defects with stem cells and compositions comprising stem cells.

Use of isolated placental stem cells in the manufacture of a medicament for treatment of bone defect

Provided herein are methods of using adherent placental stem cells and placental stem cell populations, and methods of culturing, proliferating and expanding the same. Also provided herein are methods of differentiating the placental stem cells. Further provided herein are methods of using the placental stem cells to formulate implantable or injectable compositions suitable for administration to a subject. Still further provided herein are provides methods for treating bone defects with stem cells and compositions comprising stem cells.

Use of particles of a biocompatible and bioresorbable calcium salt as an active ingredient in the preparation of a medicament for the local treatment of bone demineralizing diseases.

Utilisation d'au moins un sel de calcium biocompatible et biorésorbable, sous forme de particules, ayant des dimensions inférieures à 8 mm, comme ingrédient actif dans la préparation d'un médicament destiné au traitement local, par implantation dans la partie spongieuse ou le canal médullaire de l'os, des maladies liées à la déminéralisation ou à des troubles de minéralisation de l'os, en particulier dans le but de relancer le processus de reminéralisation de l'os et de reconstruction des travées osseuses résorbées. Par exemple, chez les malades atteints d'ostéoporose, ce traitement permet d'observer une relance du processus de reminéralisation et la reconstruction des travées osseuses, avec une augmentation très importante de la masse osseuse de l'os traité. Use of at least one biocompatible and bioresorbable calcium salt, in the form of particles, having dimensions less than 8 mm, as an active ingredient in the preparation of a medicament intended for local treatment, by implantation in the spongy part or the canal bone marrow, diseases related to demineralization or bone mineralization disorders, in particular with the aim of restarting the process of remineralization of the bone and reconstruction of resorbed bone trabeculae. For example, in patients with osteoporosis, this treatment makes it possible to observe a revival of the remineralization process and the reconstruction of the bone trabeculae, with a very significant increase in the bone mass of the treated bone.

CELL CULTURE SUPPORT AND METHOD FOR CULTIVATING CELLS.

MATERIAL FOR CELL CULTURE, METHODS OF PREPARATION AND USES THEREOF

La présente invention porte sur un matériau tridimensionnel à double porosité, comprenant un support constitué d'un polymère biorésorbable dont la surface comporte des charges positives et est fonctionnalisée par des molécules bioactives comportant des charges négatives et adaptées à augmenter la prolifération de cellules eucaryotes. Elle porte également sur son procédé de préparation ainsi que sur ses utilisations pour fixer et stimuler la prolifération de cellules eucaryotes in vitro, ex-vivo ou in vivo.

APATITIS TRICHITE AND METHOD FOR ITS PREPARATION.

Regeneration and augmentation of bone using mesenchymal stem cells

Disclosed are compositions and methods for augmenting bone formation by administering isolated human mesenchymal stem cells (hMSCs) with a ceramic material or matrix or by administering hMSCs; fresh, whole marrow; or combinations thereof in a resorbable biopolymer which supports their differentiation into the osteogenic lineage. Contemplated is the delivery of (i) isolated, culture-expanded, human mesenchymal stem cells; (ii) freshly aspirated bone marrow; or (iii) their combination in a carrier material or matrix to provide for improved bone fusion area and fusion mass, when compared to the matrix alone. The material or matrix can be a granular ceramic or three-dimensionally formed ceramic implant. The material or matrix can also be a resorbable biopolymer. The resorbable biopolymer is an absorbable gelatin, colagen or cellulose matrix, can be in the form of a powder or sponge, and is preferably a bovine skin-derived gelatin. The implants can be shaped as a cube, cylinder, block or an anatomical site. The compositions and methods can further include administering a bioactive factor such as a synthetic glucocorticoid, like dexamethasone, or a bone morphogenic protein, like BMP-2, BMP-3, BMP-4, BMP-6 and BMP-7.

Retroviral vector for the preparation of recombinant cells capable of being implanted in vivo for therapeutic purposes.

Use of particles of a biocompatible and bioresorbable calcium salt as an active ingredient in the preparation of a medicament for the local treatment of demineralizing bone diseases.

Production of fritted tablets useful as cell culture supports comprises compressing a hydroxyapatite powder under high pressure

Process (P1) for producing fritted tablets comprises compressing a hydroxyapatite powder under a pressure of at least 100 MPa. Independent claims are also included for: (1) a process (P2) for producing fritted tablets, comprising compressing an apatite powder under a pressure of at least 100 MPa to produce a green tablet, fritting the green tablet in an atmosphere containing at least 50 vol.% oxygen, and refritting the tablet in a low-activity atmosphere; (2) fritted tablet produced by P2; (3) cell culture support comprising a flat plate made of a calcium phosphate compound having a relatively high density.

IN VITRO MODELING OF HEMATOPOIETIC STEM CELL MEDICINAL NICHES: A TOOL FOR STUDYING REGULATION OF HEMATOPOIESE, EVALUATING THE POTENTIAL OF NICHING A HEMATOPOIETIC GRAFT AND TESTING THE PHARMACO-TOXICOLOGY OF MEDICINAL PRODUCTS.

Patent FR2706768B1

PCT No. PCT/FR94/00565 Sec. 371 Date Feb. 2, 1995 Sec. 102(e) Date Feb. 2, 1995 PCT Filed May 11, 1994 PCT Pub. No. WO94/26322 PCT Pub. Date Nov. 24, 1994A method for treating a living organism includes fitting in the living organism a bioabsorbable implant. The bioabsorbable implant includes a support of porous calcium carbonate based material for supporting at least one growth factor. The calcium carbonate based support material forms an external wall of the support.

Cell engraftment method on three-dimensional structure surface

Methods and materials for nanocrystalline surface coatings and attachment of peptide amphiphile nanofibers thereon

Biocompatible composites comprising peptide amphiphiles and surface modified substrates and related methods for attachment thereon.

Regulation of hematopoietic stem cell differentiation by the use of human mesenchymal stem cells

The invention relates to the induction of hematopoietic stem cells to differentiate into osteoclasts by culturing hematopoietic stem cells with human mesenchymal stem cells, and, in a preferred embodiment, using no exogenous cytokines. Differentiation of the mesenchymal stem cells into osteoblasts inhibited the differentiation of hematopoietic stem cells into osteoclasts. In addition, hematopoietic stem cells can be genetically engineered to carry genes of interest particularly for the expression of physiologically active proteins. In the presence of mesenchymal stem cells, the transduced cells carry the new genetic material and express gene products that can be used to modulate bone resorption.

Use of biocompatible and bioabsorbable calcium salt particles as an active ingredient in the manufacture of a medicament intended for the topical treatment of bone mineral loss diseases

Activation and expansion of cells

The present invention relates generally to methods for activating and expanding cells, and more particularly, to a novel method to activate and/or stimulate cells that maximizes the expansion of such cells to achieve dramatically high densities. In the various embodiments, cells are activated and expanded to very high densities in a short period of time. In certain embodiments, cells are activated and expanded to very high numbers of cells in a short period of time. Compositions of cells activated and expanded by the methods herein are further provided.

APATITE TRICHITE AND METHOD FOR ITS PREPARATION.

L'invention concerne: une trichite d'apatite ayant un rapport d'aspect moyen (longueur le long de l'axe-c/longueur le long de l'axe-a) non inférieur à 10,0 et contenant des groupes acide carbonique en quantité non inférieure à 0,01 % en poids basé sur le poids total de la trichite. Une méthode pour préparer une trichite d'apatite comprenant la précipitation et la croissance d'un apatite a partir d'un liquide contenant l'acide phosphorique et la calcium jusqu'a l'obtention d'un rapport d'aspect moyen (longueur le long de l'axe-c/longueur de l'axe-a) non inférieur à 10,0. Disclosed is: an apatite whiskers having an average aspect ratio (length along the c-axis / length along the a-axis) of not less than 10.0 and containing carbonic acid groups in an amount not less than 0.01% by weight based on the total weight of the whiskers. A method for preparing an apatite whiskers comprising precipitating and growing an apatite from a liquid containing phosphoric acid and calcium until an average aspect ratio (length le along axis-c / length of axis-a) not less than 10.0.

Compositions for treatment of osteochondral disorders

The application provides biocompatible carriers comprising bone forming and/or cartilage forming cells (ATMPs) and methods for making them. The application further provides pharmaceutical compositions comprising said ATMPs and method of treatments using said ATMPs. The application further relates to said ATMPS for use in the treatment of bone disorders, cartilage disorders and joint disorders. The current invention further relates to method of treatments of bone disorders, cartilage disorders and joint disorders.

Functionalized artificial bone and joint compositions and methods of use and manufacture

Modular synthetic tissue-graft scaffold

A modular synthetic tissue-graft scaffold (10) includes one or more nominally identical scaffold cages (12) configured to facilitate regrowth of tissue of an organism in and around the scaffold cages. Each scaffold cage comprises a volumetric enclosure (18) bounded by a perforated wall structure (40). A recess (24) formed at one end of the volumetric enclosure defines an inner stepped coupling surface. An annular raised portion (26) positioned at the other end of the volumetric enclosure forms an outwardly projecting stepped seating surface sized to form a complementary matable surface to the inner stepped coupling surface for whenever an inner stepped coupling surface of another one of the cages is placed on the outer stepped seating surface of the scaffold cage. Corridors (46) extending through the perforated wall structure and communicating with passageways (54) within the volumetric enclosure enable migration of material within and out of the scaffold cage.

Bioreactor for cell self-assembly in form of an organ copy; procedures for the production and the application of cell culture, differentiation, maintenance, proliferation and/or use of cells

The invention concerns a bioreactor in form of an organ copy representing the typical structures of animal or human organs; a procedure to manufacture as well as use the bioreactor for the cultivation, differentiation, maintenance, proliferation and/or use of organ cells or stem cells. The characteristic of this invention is that the specific hollow pathway structures supplying the cells in the open pore body are the exact same configuration as they occur in a natural organ. A further characteristic is the cell culture within open-porous structures, being perfused between the hollow pathway structures, branching out from the center to the periphery and branching in from the periphery to the center. With this bioreactor a device is described that facilitates the reorganization and use of microorganisms or cells in a manner typical to that of the natural organ.

Neomorphogenesis of cartilage in vivo from cell culture

Methods and artificial matrices for the growth and implantation of cartilaginous structures and surfaces and bone are disclosed. In the preferred embodiments, chondrocytes are grown on biodegradable, biocompatible fibrous polymeric matrices. Optionally, the cells are proliferated in vitro until an adequate cell volume and density has developed for the cells to survive and proliferate in vivo. One advantage of the matrices is that they can be cast or molded into a desired shape, on an individual basis, so that the final product closely resembles a patient's own ear or nose. Alternatively, flexible matrices can be used which can be manipulated at the time of implantation, as in a joint, followed by remodeling through cell growth and proliferation in vivo. The cultured cells can also be maintained on the matrix in a nutrient media for production of bioactive molecules such as angiogenesis inhibiting factor. Examples are provided showing the growth of hyaline cartilage for joint relinings, the growth of elastic cartialage for plastic or reconstructive replacement of cartilage structures, and repair of large bone defects.

BIOMATERIALS FOR THE PREVENTION AND TREATMENT OF TISSUE DISORDERS

Reivindicación 1: Un biomaterial estéril y desecado que comprende células diferenciadas desvitalizadas que tienen propiedades regeneradoras y/o reparadoras de tejidos, y una gelatina, estando las células y gelatina incrustados en una matriz extracelular. Reivindicación 17: Un biomaterial según una cualquiera de las reivindicaciones 1 a 11, según la reivindicación 14, o una composición farmacéutica según la reivindicación 15, para uso como medicamento. Reivindicación 22: El biomaterial o la composición farmacéutica para su uso de acuerdo con la reivindicación 17 ó 18, para compensar los efectos secundarios de un tratamiento terapéutico que se sabe que tiene un efecto deletéreo sobre los tejidos, en particular tejido óseo, tejido cartilaginoso, tejido cutáneo, tejido muscular, tejido endotelial, y tejido adiposo. Claim 1: A sterile and desiccated biomaterial comprising devitalized differentiated cells having tissue regenerating and/or repairing properties, and a gelatin, the cells and gelatin being embedded in an extracellular matrix. Claim 17: A biomaterial according to any one of claims 1 to 11, according to claim 14, or a pharmaceutical composition according to claim 15, for use as a medicament. Claim 22: The biomaterial or the pharmaceutical composition for use according to claim 17 or 18, to compensate for the secondary effects of a therapeutic treatment that is known to have a deleterious effect on tissues, in particular bone tissue, cartilage tissue, skin tissue, muscle tissue, endothelial tissue, and adipose tissue.

Regulation of hematopoietic stem cell differentiation by the use of human mesenchymal stem cells

The invention relates to the induction of hematopoietic stem cells to differentiate into osteoclasts by culturing hematopoietic stem cells with human mesenchymal stem cells, and, in a preferred embodiment, using no exogenous cytokines. Differentiation of the mesenchymal stem cells into osteoblasts inhibited the differentiation of hematopoietic stem cells into osteoclasts. In addition, hematopoietic stem cells can be genetically engineered to carry genes of interest particularly for the expression of physiologically active proteins. In the presence of mesenchymal stem cells, the transduced cells carry the new genetic material and express gene products that can be used to modulate bone resorption.

USE OF POROUS CALCIUM CARBONATE AS A SUPPORT MATERIAL FOR THE IN VITRO CULTURE OF EUKARYOTIC CELLS.

Production of high porosity solid useful as bone substitute

A highly porous product is produced by mixing a powder or granulate with a solution of an Al, Ca, Mg, P, Si, Ti or Zr oxide in specific amounts and drying the gel obtained. Production of a highly porous solid comprises: (a) homogeneously mixing 0.2 g to 12 g of a powder or granulate with 1 ml of a solution of an oxide of Al, Ca, Mg, P, Si, Ti or Zr in water or an alcohol, preferably water-ethanol, in which the oxide:solvent molar ratio is 1:4-30; (b) drying the moist granulate obtained, preferably at room temperature, until about 90% of solvent has been removed; and (c) heating the dried granulate at 200 deg C after heating up at a rate of 1 degree/minute.

APATIT WHISKER AND METHOD FOR THEIR PRODUCTION

Cell culture base

Compositions comprising citrate and applications thereof

In one aspect, methods of promoting bone growth are described herein. In some embodiments, a method of promoting bone growth described herein comprises promoting cell differentiation or phenotype progression in a population of bone cells by providing a citrate-presenting composition to the population of bone cells. In some embodiments, the citrate-presenting composition is provided to the bone cells at a first stage of cell development selected to obtain a first cell differentiation or phenotype progression. Additionally, in some cases, a second citrate-presenting composition is further provided to the bone cells at a second stage of cell development selected to obtain a second cell differentiation or phenotype progression.

Apatite whisker and method for producing the same

Regeneration and augmentation of bone using mesenchymal stem cells

Disclosed are compositions and methods for augmenting bone formation by administering isolated human mesenchymal stem cells (hMSCs) with a ceramic material or matrix or by administering hMSCs; fresh, whole marrow; or combinations thereof in a resorbable biopolymer which supports their differentiation into the osteogenic lineage. Contemplated is the delivery of (i) isolated, culture-expanded, human mesenchymal stem cells; (ii) freshly aspirated bone marrow; or (iii) their combination in a carrier material or matrix to provide for improved bone fusion area and fusion mass, when compared to the matrix alone. The material or matrix can be a granular ceramic or three-dimensionally formed ceramic implant. The material or matrix can also be a resorbable biopolymer. The resorbable biopolymer is an absorbable gelatin, collagen or cellulose matrix, can be in the form of a powder or sponge, and is preferably a bovine skin-derived gelatin. The implants can be shaped as a cube, cylinder, block or an anatomical site. The compositions and methods can further include administering a bioactive factor such as a synthetic glucocorticoid, like dexamethasone, or a bone morphogenic protein, like BMP-2, BMP-3, BMP-4, BMP-6 and BMP-7.

Multi-purpose substrates useful for cell culture and methods for making

Described herein are multi-purpose substrates composed of (1) a base coated with a calcium phosphate coating and (2) a fluorophore-labeled collagen adsorbed on the calcium phosphate coating. The multi-purpose substrates are useful in culturing and studying the activity of a variety of cells. The multi-purpose substrates described herein can be used for both solution- and image-based analysis of cultured cells. New methods for producing and using such coated substrates are also disclosed.

Bioactive soft tissue implant and methods of manufacture and use thereof

A bioactive filamentary structure includes a sheath coated with a mixture of synthetic bone graft particles and a polymer solution forming a scaffold structure. In forming such a structure, synthetic bone graft particles and a polymer solution are applied around a filamentary structure. A polymer is precipitated from the polymer solution such that the synthetic bone graft particles and the polymer coat the filamentary structure and the polymer is adhered to the synthetic bone graft particles to retain the graft particles.

Method for Surface Modification of Polymeric Scaffold for Stem Cell Transplantation Using Decellularized Extracellular Matrix

The present invention relates to a method for the surface modification of a polymeric scaffold for stem cell transplantation using a decellularized extracellular matrix. The method for the surface modification of the polymeric scaffold according to the present invention can embody a biomimetic surface environment that is effective for initial cell attachment, cell growth and differentiation of stem cells by modifying the surface of the polymeric scaffold using the decellularized extracellular matrix directly derived from specific tissue cells.

Compositions comprising citrate and applications thereof

In one aspect, methods of promoting bone growth are described herein. In some embodiments, a method of promoting bone growth described herein comprises promoting cell differentiation or phenotype progression in a population of bone cells by providing a citrate- presenting composition to the population of bone cells. In some embodiments, the citrate- presenting composition is provided to the bone cells at a first stage of cell development selected to obtain a first cell differentiation or phenotype progression. Additionally, in some cases, a second citrate-presenting composition is further provided to the bone cells at a second stage of cell development selected to obtain a second cell differentiation or phenotype progression.

Cell culture support embeddable in vivo

A cell culture support implantable in vivo in which an acidic collagen solution having a concentration of from 5 to 20 mg/mL and having been passed through a filter with a pore size of 10 μm or less has been neutralized and which has independent pores having an opening size of from 100 μm to 1000 μm on the surface thereof. In particular, an implantable cell culture support having such a shape and/or a structure as undergoing sufficiently self-organized accumulation which are obtained by conducting the above-described neutralization under directionally supplying an alkali.

Method for culturing pearls

Activation and expansion of cells

The present invention relates generally to methods for activating and expanding T cells, and more particularly, to a novel method to activate and/or stimulate T cells that maximizes the expansion of such cells to achieve dramatically high densities. In the various embodiments, cells are activated and expanded to very high densities in a short period of time. In certain embodiments, cells are activated and expanded to very high numbers of cells in a short period of time. Compositions of cells activated and expanded by the methods herein are further provided.

Method and device for producing biological tissue in a growth chamber

The invention relates to a method and device for producing tissue in a growth chamber for grafting into or onto human or animal bodies. One particular advantage of the invention is that it produces structurally functional bones. According to the invention, biological cells are disposed on a growth frame and arranged together in a growth chamber. Structured and functional growth is obtained by targeted, chronically or locally differing effect of biologically active stimuli. Said stimuli can be mechanical, electrical, magnetical, chemical, olfactive, acoustic and/or optical. According to the invention, the application of stimuli which correspond to those which are normally applied to the natural tissue in the body, is the most suitable.

In vitro modelling of haematopoietic stem cell medullary nests: a tool for studying the regulation of haematopoiesis, evaluating the nesting potential of a haematopoietic graft and testing the pharmacotoxicology of medicaments

The present invention relates to a culture substrate for haematopoietic stem cells (HSCs) and/or haematopoietic progenitors (HPs), comprising a calcium biomaterial, osteoclasts, endothelial cells and mesenchymal stem cells (MSCs) and/or osteoblasts and/or adipocytes. The present invention also relates to a method for preparing such a culture substrate, and a method for the in vitro culturing of HSCs and/or HPs. The use of such a culture substrate for studying the cellular mechanisms involved in haematopoiesis and/or the differentiation of HSCs/HPs, and/or for studying the efficacy and/or the toxicity of a candidate medicament is also described.

Microcellular polymers as cell growth media and novel polymers

Microcellular polyhipe polymer scaffold suitable for growth of living matter for biomedical applications, obtainable by polymerising a high internal phase emulsion, comprising a homogeneous cross-linked open cellular material defined by a bulk polymer matrix having a surface and an interface with an internal phase, and having porosity greater than 75 % comprising emulsion derived pores of diameter in the range of 0.1 to 10,000 micron and emulsion derive pore interconnects of diameter in the range of up to 100 micron, wherein the scaffold comprises a plurality of discrete and/or interpenetrating zones: at the polymer surface; within its bulk matrix; at the interface between polymer and internal phase; and/or between adjacent but distinct pores and/or interconnects, characterised by form and dimension of pore and interconnect type within each zone, and location of zones wherein adjacent or interpenetrating zones are distinguished by boundaries which may be between or contained within adjacent pores and/or interconnects in respective zones, whereby zones are suitable for regulating positioning and/or morphology of living matter; a biologically active system, or organ support module comprising the scaffold and methods and processes for preparation thereof and use thereof.

In vitro cell culture system

In vitro cell culture systems for anchorage-dependent mammalian cells using solid substrates of mitogenic calcium compounds, such as hydroxyapatite and tricalcium phosphate forms of calcium phosphate and calcium carbonate. The calcium solid substrates can be in the form of granules with a particle size of at least 0.050 mm. or in the form of solid bodies and may be either porous or non-porous. Unique features of cells cultured in the stated in vitro cell culture systems include the growth of cells in layers many cells thick, growth of cells that maintain their phenotype and the ability to culture cells for extended periods of time.

Methods and compositions for treatment of bone defects with placental cell populations

Provided herein are methods of using adherent placental stem cells and placental stem cell populations, and methods of culturing, proliferating and expanding the same. Also provided herein are methods of differentiating the placental stem cells. Further provided herein are methods of using the placental stem cells to formulate implantable or injectable compositions suitable for administration to a subject. Still further provided herein are provides methods for treating bone defects with stem cells and compositions comprising stem cells.

Activation and expansion of cells

The present invention relates generally to methods for activating and expanding cells, and more particularly, to a novel method to activate and/or stimulate cells that maximizes the expansion of such cells to achieve dramatically high densities. In the various embodiments, cells are activated and expanded to very high densities in a short period of time. In certain embodiments, cells are activated and expanded to very high numbers of cells in a short period of time. Compositions of cells activated and expanded by the methods herein are further provided.

Production of dentin, cementum and enamel by cells

One aspect provides a method of forming a mineralized material by co-culturing a epithelial cell, such as ameloblasts, and mesenchymal cells, such as osteoblasts or odontoblasts, in a mineral-stimulating medium. Another aspect provides matrix seeded with epithelial cells and mesenchymal cells and infused with a mineral-stimulating medium capable of forming a mineralized material in the matrix. Methods of manufacturing such compositions and methods of treating mineralization-related conditions are also provided.