08-08-2013 дата публикации
Номер: US20130205444A1
Transgenic soybean seed having increased total fatty acid content of at least 10% and altered fatty acid profiles when compared to the total fatty acid content of non-transgenic, null segregant soybean seed are described. DGAT genes from oleaginous organisms are used to achieve the increase in seed storage lipids. 1. An isolated polynucleotide comprising:(a) a nucleotide sequence encoding a polypeptide having diacylglycerol acyltransferase activity wherein the polypeptide has at least 80% amino acid identity, based on the Clustal V method of alignment, when compared to an amino acid sequence as set forth in SEQ ID NOs:135, 136, 162, 176, 215, 234, 265, 272, 279, 299, 304, 306, 310, 312, 314, 316, 318, 320, 322, 351, or 363;(b) a nucleotide sequence encoding a polypeptide having diacylglycerol acyltransferase activity, wherein the nucleotide sequence has at least 80% sequence identity, based on the BLASTN method of alignment, when compared to a nucleotide sequence as set forth in SEQ ID NO: 133, 134, 161, 175, 214, 233, 264, 271, 278, 298, 301, 303, 305, 309, 311, 313, 315, 317, 319, 321, 350, or 352;(c) a nucleotide sequence encoding a polypeptide having diacylglycerol acyltransferase activity, wherein the nucleotide sequence hybridizes under stringent conditions to a nucleotide sequence as set forth in SEQ ID NO: 133, 134, 161, 175, 214, 233, 264, 271, 278, 298, 301, 303, 305, 309, 311, 313, 315, 317, 319, 321, 350, or 352; or(d) a complement of the nucleotide sequence of (a), (b) or (c), wherein the complement and the nucleotide sequence consist of the same number of nucleotides and are 100% complementary.2. The polypeptide of claim 1 , wherein the amino acid sequence of the polypeptide has at least 85% sequence identity claim 1 , based on the Clustal V method of alignment claim 1 , when compared to SEQ ID NO: 135 claim 1 , 136 claim 1 , 162 claim 1 , 176 claim 1 , 215 claim 1 , 234 claim 1 , 265 claim 1 , 272 claim 1 , 279 claim 1 , 299 claim 1 , 304 claim 1 , ...
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