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Небесная энциклопедия

Космические корабли и станции, автоматические КА и методы их проектирования, бортовые комплексы управления, системы и средства жизнеобеспечения, особенности технологии производства ракетно-космических систем

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Мониторинг СМИ

Мониторинг СМИ и социальных сетей. Сканирование интернета, новостных сайтов, специализированных контентных площадок на базе мессенджеров. Гибкие настройки фильтров и первоначальных источников.

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Поддерживает ввод нескольких поисковых фраз (по одной на строку). При поиске обеспечивает поддержку морфологии русского и английского языка
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Применить Всего найдено 9505. Отображено 100.
05-01-2012 дата публикации

Plant diacylglycerol acyltransferases

Номер: US20120004125A1
Автор: Anthony J. Kinney, Edgar Benjamin Cahoon, Karlene H. Butler, Rebecca E. Cahoon
Принадлежит: EI Du Pont de Nemours and Co

This invention relates to an isolated nucleic acid fragment encoding a diacylglycerol acyltransferase. The invention also relates to the construction of a chimeric gene encoding all or a portion of the diacylglycerol acyltransferase, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the diacylglycerol acyltransferase in a transformed host cell.

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Номер записи: 1
19-01-2012 дата публикации

Enzymes and methods for producing omega-3 fatty acids

Номер: US20120016144A1
Автор: Anne Maree Mackenzie, Craig Christopher Wood, Dion Matthew Frederick Frampton, James Robertson Petrie, Peter David Nichols, Peter Maged Mansour, Pushkar Shrestha, Qing Liu, Stanley Suresh Robert, Surinder Pal Singh, Susan Irene Ellis Blackburn, Xue-Rong Ziiou
Принадлежит: Individual

The present invention relates generally to the field of recombinant fatty acid synthesis, particularly in transgenic plants. The application describes genes involved in fatty acid synthesis and provides methods and vectors for the manipulation of fatty acid composition of plant oils. In particular, the invention provides constructs for achieving the integration of multiple heterologous genes involved in fatty acid synthesis into the plant genome, such that the resulting plants produce altered levels of polyunsaturated fatty acids. Also described are methods for enhancing the expression of fatty acid biosynthesis enzymes by co-expressing a silencing suppressor within the plant storage organ.

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Номер записи: 2
19-01-2012 дата публикации

Process for the production of arachidonic acid and/or eicosapentaenoic acid

Номер: US20120017332A1
Автор: Johnathan A. Napier, Olga Sayanova
Принадлежит: BASF Plant Science GmbH

The present invention relates to a new process for the production of arachidonic acid and/or eicosapentaenoic acid in plants through the co-expression of a Δ-12-/Δ-15-desaturase, Δ-9-elongase, Δ-8-desaturase and a Δ-5-desaturase and a process for the production of lipids or oils having an increased content of unsaturated fatty acids, in particular ω-3 and ω-6 fatty acids having at least two double bonds and a 18 or 20 carbon atom chain length. Preferably the arachidonic acid and eicosapentaenoic acid are produced in at least a 1:2 ratio. The invention furthermore relates to the production of a transgenic plants, preferably a transgenic crop plant, having an increased content of arachidonic acid and/or eicosapentaenoic acid, oils or lipids containing C 18 - or C 20 -fatty acids with a double bond in position Δ5, 8, 9, 11, 12, 14, 15 or 17 of the fatty acid produced, respectively due to the expression of the Δ-12-/Δ-15-desaturase, of the Δ-9-elongase, of the Δ-8-desaturase and of the Δ-5-desaturase in the plant. The expression of the inventive Δ-12-/Δ-15-desaturase leads preferably to linoleic acid and linolenic acid as products having a double bond in the position

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Номер записи: 3
02-02-2012 дата публикации

Preparation of adipic acid

Номер: US20120028320A1
Автор: Axel Christoph Trefzer, Martin SCHÜRMANN, Petronella Catharina Raemakers-Franken, Stefaan Marie Andre De Wildeman
Принадлежит: DSM IP ASSETS BV

The invention relates to a method for preparing adipic acid, comprising converting alpha-ketoglutaric acid (AKG) into alpha-ketoadipic acid (AKA), converting alpha-ketoadipic acid into alpha-ketopimelic acid (AKP), converting alpha-ketopimelic acid into 5-formylpentanoic acid (5-FVA), and converting 5-formylpentanoic acid into adipic acid, wherein at least one of these conversions is carried out using a heterologous biocatalyst.The invention further relates to a heterologous cell, comprising one or more heterologous nucleic acid sequences encoding one or more heterologous enzymes capable of catalysing at least one reaction step in said method.

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Номер записи: 4
02-02-2012 дата публикации

Yeast organism producing isobutanol at a high yield

Номер: US20120028323A1
Автор: Aristos Aristidou, Catherine Asleson Dundon, Christopher Smith, Jun URANO, Peter Meinhold, Reid M. Renny FELDMAN, Uvini GUNAWARDENA
Принадлежит: Gevo Inc

The present invention provides recombinant microorganisms comprising an isobutanol producing metabolic pathway and methods of using said recombinant microorganisms to produce isobutanol. In various aspects of the invention, the recombinant microorganisms may comprise a modification resulting in the reduction of pyruvate decarboxylase and/or glycerol-3-phosphate dehydrogenase activity. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.

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Номер записи: 5
01-03-2012 дата публикации

Recombinant microbial host cells for high eicosapentaenoic acid production

Номер: US20120052537A1
Автор: Hongxiang Zhang, Narendra S. Yadav, Pamela L. Sharpe, Quinn Qun Zhu, Seung-Pyo Hong, Zhixiong Xue
Принадлежит: EI Du Pont de Nemours and Co

Engineered strains of the oleaginous yeast Yarrowia lipolytica are disclosed herein that are capable of producing microbial oil comprising greater than 25 weight percent of eicosapentaenoic acid [“EPA”], an omega-3 polyunsaturated fatty acid, measured as a weight percent of dry cell weight.

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Номер записи: 6
15-03-2012 дата публикации

Enzymatic Oil-Degumming Method

Номер: US20120064192A1
Автор: Jorn Borch Soe, Mark Turner
Принадлежит: DUPONT NUTRITION BIOSCIENCES APS

A process of enzymatic degumming edible oils, comprising treating edible oil with a lipid acyltransferase so as to transfer an acyl group from a major part of the phospholipid to one or more acyl acceptors, wherein the acyl acceptor may be any compound comprising a hydroxyl group. In one embodiment preferably the acyl acceptor is water and in another embodiment preferably the acyl acceptor is one or more sterols and/or stanols. When the acyl acceptor is a stanol and/or sterol, one or more sterol esters and/or stanol esters are produced. The lipid acyltransferase for use in the process of the present invention may comprise one or more of the following amino acid sequences: SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, or SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 36, SEQ ID NO: 38, SEQ ID NO: 40, SEQ ID NO: 41, SEQ ID NO: 45, SEQ ID NO: 47, SEQ ID NO: 50 or an amino acid sequence which has 75% or more identity thereto. A novel lipid acyltransferase comprising the amino acid sequence shown as SEQ ID NO: 16 is also taught.

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Номер записи: 7
19-04-2012 дата публикации

Dgat genes from oleaginous organisms for increased seed storage lipid production and altered fatty acid profiles in oilseed plants

Номер: US20120096588A1
Автор: Howard Glenn Damude, Kevin G. Ripp, Kevin L. Stecca, Knut Meyer
Принадлежит: EI Du Pont de Nemours and Co

Transgenic soybean seed having increased total fatty acid content of at least 10% and altered fatty acid profiles when compared to the total fatty acid content of non-transgenic, null segregant soybean seed are described. DGAT genes from oleaginous organisms are used to achieve the increase in seed storage lipids.

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Номер записи: 8
03-05-2012 дата публикации

Use of LCAT for Treating Anemia and Red Blood Cell Dysfunction

Номер: US20120107298A1
Автор: Brian Krause, Bruce J. Auerbach, Reynold Homan
Принадлежит: Alphacore Pharma LLC

Disclosed are methods for treating conditions characterized by anemia or red blood cells dysfunction by administering an agent that increases the level of endogenous LCAT or LCAT activity. Additionally disclosed are methods of treating conditions wherein red blood cells have reduced function in relation to deformability, oxygenation, increased adhesion and aggregability, reduced nitric oxide function, or decreased life-span, increased free cholesterol, or abnormal phospholipid content. Also disclosed are methods for treating conditions characterized by an abnormal concentration of free cholesterol in red blood cells and methods of normalizing the free cholesterol content of red blood cells.

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Номер записи: 9
14-06-2012 дата публикации

Mesophilic and Thermophilic Organisms Modified to Produce Acrylate, and Methods of Use Thereof

Номер: US20120149077A1
Автор: Arthur J. Shaw, IV, Vineet Rajgarhia
Принадлежит: Mascoma Corp

The present invention provides for novel metabolic pathways leading to acrylate formation in a consolidated bio-processing system (CBP) where lignocellulosic biomass is efficiently converted to acrylate. In one such metabolic pathway, pyruvate is converted to lactate, which is converted to lactoyol-CoA, which is converted to acryloyl-CoA, and which is finally converted to acrylate. In another such metabolic pathway, pyruvate is converted to L-α-alanine, which is converted to L-aspartate, which is converted to β-alanine, which is converted to β-alanyl-CoA, which is converted to acryloyl-CoA, and which is finally converted to acrylate. In yet another metabolic pathway, pyruvate is converted to lactate, and then lactate is converted directly to acrylate. In certain aspects, the invention provides for heterologous expression of one or more enzymes in a mesophilic or thermophilic organism, such as Thermoanaerobacterium saccharolyticum or Clostridium thermocellutn , where the one or more enzymes functions within a novel metabolic pathway as described above to convert pyruvate to acrylate via lactate, or via β alanine and acryloyl-CoA.

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Номер записи: 10
21-06-2012 дата публикации

Preparation of alpha-ketopimelic acid

Номер: US20120156737A1
Автор: Axel Christoph Trefzer, Martin SCHÜRMANN, Petronella Catharina Raemakers-Franken, Stefaan Marie Andre De Wildeman
Принадлежит: DSM IP ASSETS BV

The invention relates to a method for preparing alpha-ketopimelic acid, comprising converting alpha-ketoglutaric acid into alpha-ketoadipic acid and converting alpha-ketoadipic acid into alpha-ketopimelic acid, wherein at least one of these conversions is carried out using a heterologous biocatalyst. The invention further relates to a heterologous cell, comprising one or more heterologous nucleic acid sequences encoding one or more heterologous enzymes capable of catalysing at least one reaction step in the preparation of alpha-ketopimelic acid from alpha-ketoglutaric acid.

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Номер записи: 11
12-07-2012 дата публикации

Novel delta-9 fatty acid elongase genes and their use in making polyunsaturated fatty acids

Номер: US20120177805A1
Автор: Anthony J. Kinney, Brian Mcgonigle, Howard Glenn Damude
Принадлежит: Individual

Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding novel delta-9 elongases along with a method of making long-chain polyunsaturated fatty acids (PUFAs) using these delta-9 elongases in plants.

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Номер записи: 12
12-07-2012 дата публикации

Mutated acetohydroxyacid synthase genes in brassica

Номер: US20120178628A1
Автор: Christian Schopke, Greg F. W. Gocal, Keith Walker, Peter R. Beetham
Принадлежит: Individual

Provided are mutated acetohydroxyacid synthase (AHAS) nucleic acids and the proteins encoded by the mutated nucleic acids. Also provided are canola plants, cells, and seeds comprising the mutated genes.

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Номер записи: 13
26-07-2012 дата публикации

Genes for Enhanced Lipid Metabolism for Accumulation of Lipids

Номер: US20120190115A1
Автор: Bertrand Vick, Oliver Kilian
Принадлежит: Aurora Algae Inc

Provided herein are exemplary genes, constructs and methods for the formation of triacylglycerols (TAGs). The exemplary genes include a phosphatic acid phosphohydrolase (PA Hydrolase) gene, a diacylglycerol o-acyltransferase (DAGAT2A) gene, and a phospholipid:diacylglycerol acyltransferase (LROI) gene.

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Номер записи: 14
25-10-2012 дата публикации

Modification of enzymatic crosslinkers for controlling properties of crosslinked matrices

Номер: US20120270810A1
Автор: Guy Tomer, Orahn Preiss-Bloom
Принадлежит: Lifebond Ltd

Improved matrix or hydrogel that is formed by enzymatic crosslinking of polymers wherein the crosslinking enzyme molecules have been modified for the purpose of improving the crosslinking density, mechanical properties, or other properties of the matrix, and/or to provide improved control over the rate and/or extent of crosslinking, wherein the enzyme molecules are modified to alter the perceived volume of the enzyme molecules in the crosslinked matrix being formed. Methods of production and of use are also provided.

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Номер записи: 15
01-11-2012 дата публикации

Diacylglycerol acyltransferase genes and use thereof

Номер: US20120277451A1
Автор: Misa Ochiai
Принадлежит: Suntory Holdings Ltd

It is an object to provide a novel diacylglycerol acyltransferase. The present invention relates to a diacylglycerol acyltransferase, a polynucleotide encoding the same, and so on. The present invention provides a polynucleotide comprising the nucleotide sequence of, e.g., SEQ ID NO: 1 or 4, a polynucleotide encoding a protein consisting of the amino acid sequence of SEQ ID NO: 2, an expression vector and transformant comprising the polynucleotide, a method for producing a lipid or fatty acid composition using the transformant, or a food, etc. comprising the lipid or fatty acid produced by the method.

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Номер записи: 16
15-11-2012 дата публикации

Crystal structure of glyphosate acetyltransferase (glyat) and methods of use

Номер: US20120288914A1
Автор: Daniel Siehl, Linda A. Castle, Robert J. Keenan, Zhenglin Hou
Принадлежит: PIONEER HI BRED INTERNATIONAL INC

The presently disclosed subject matter provides compositions and methods for evaluating the potential of candidate polypeptides to associate with glyphosate with a higher binding affinity, higher binding specificity, or both or to have N-acetyltransferase activity with a higher catalytic rate when compared to a native glyphosate acetyltransferase (GLYAT) polypeptide through the provision and comparison of three-dimensional molecular structures of the candidate polypeptides and the GLYAT polypeptides provided herein. The methods further provide for identification of polypeptides with these advantageous properties using the three-dimensional molecular structures of GLYAT polypeptides.

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Номер записи: 17
15-11-2012 дата публикации

Optimized strains of yarrowia lipolytica for high eicosapentaenoic acid production

Номер: US20120289600A1
Автор: Narendra S. Yadav, Quinn Qun Zhu, Zhixiong Xue
Принадлежит: EI Du Pont de Nemours and Co

Engineered strains of the oleaginous yeast Yarrowia lipolytica capable of producing greater than 50 weight percent of eicosapentaenoic acid [“EPA”], an ω-3 polyunsaturated fatty acid, in the total oil fraction are described. These strains over-express heterologous Δ9 elongases, Δ8 desaturases, Δ5 desaturases, Δ17 desaturases, Δ12 desaturases and C 16/18 elongases, and optionally over-express diacylglycerol cholinephosphotransferases. Preferred gene knockouts are also described. Production host cells, methods for producing EPA within said host cells, and products comprising EPA from the optimized Yarrowia lipolytica strains are claimed.

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Номер записи: 18
06-12-2012 дата публикации

Thermophilic thermoanaerobacter italicus subsp. marato having high alcohol productivity

Номер: US20120309065A1
Автор: Marie Just Mikkelsen, Rasmus Lund ANDERSEN, Thomas Kvist
Принадлежит: BIOGASOL IPR APS

Strict anaerobic thermophilic bacterium belonging to the group of Thermoanaerobacter italicus subsp. marato subsp. nov. and mutants and derivatives thereof. The bacterium is particularly suitable for the production of fermentation products such as ethanol, lactic acid, acetic acid and hydrogen from lignocellulosic biomass.

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Номер записи: 19
17-01-2013 дата публикации

Nucleic acid construct comprising pyripyropene biosynthetic gene cluster and marker gene

Номер: US20130017581A1
Автор: Hiroyuki Anzai, Kentaro Yamamoto, Koichiro Murashima, Kouji Yanai, Naomi Sumida, Sato Aihara
Принадлежит: Individual

There is provided a nucleic acid construct comprising a pyripyropene biosynthetic gene cluster and a marker gene. The nucleic acid construct according to the present invention provides an inexpensive and highly productive method for producing pyripyropene.

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Номер записи: 20
07-02-2013 дата публикации

Lipid acyltransferase proteins and methods of making them

Номер: US20130034627A1
Автор: Birgitte Ø. Wittschieben, Charlotte Horsmans Poulsen, Charlotte Johansen Vedel, Charlotte R. Thoudahl, Janne Brunstedt, Jens Frisbæk SØRENSEN, Jorn Borch Soe, Jorn Dalgaard Mikkelsen, Lene B. Jensen, Lone B. Miller, Marguerite A. Cervin, Morten K. Larsen, Richard R. Bott, Rikke H. Lorentzen
Принадлежит: DUPONT NUTRITION BIOSCIENCES APS

The present invention provides a method for preparing a variant lipid acyltransferase enzyme by expressing a nucleotide sequence encoding a lipid acyltransferase which may comprise at least one modification at a position(s) which corresponds in the encoded amino acid sequence to an amino acid(s) located in a) the canyon region of the enzyme (i.e. preferably amino acid residues 31, 27, 85, 86, 119, and 120); and/or b) insertion site 1 (i.e. amino acid residues 22-36) and/or c) insertion site 2 (i.e. amino acid residues 74-88), wherein the canyon region, insertion site 1 and/or insertion site 2 are defined as that region which when aligned based on primary or tertiary structure corresponds to the canyon region, insertion site 1 or insertion site 2 (or the corresponding amino acid residues taught above) of the enzyme shown herein as SEQ ID No. 16 or 6 in a host organism.

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Номер записи: 21
07-02-2013 дата публикации

Cells, nucleic acids, enzymes and use thereof, and methods for the production of sophorolipids

Номер: US20130035403A1
Автор: Anja Thiessenhusen, Mirja Wessel, Steffen Schaffer
Принадлежит: EVONIK DEGUSSA GmbH

The invention relates to cells, nucleic acids, and enzymes, the use thereof for producing sophorolipids, and methods for producing sophorolipids.

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Номер записи: 22
14-03-2013 дата публикации

Cell-free preparation of carbapenems

Номер: US20130065878A1
Автор: Daniel Klein-Marcuschamer, William Jeremy Blake
Принадлежит: Greenlight Biosciences Inc

Provided herein are cell-free systems for generating carbapenems, e.g., a compound of the Formula (I): or salts thereof; wherein , R 1 , R 2 , R 3 , R 4 , R 5 , and R 6 are defined herein. Also provided are pharmaceutical compositions comprising a compound generated by the inventive cell-free system, and use of these compounds and compositions for the treatment of bacterial infections.

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Номер записи: 23
28-03-2013 дата публикации

HUMAN DIACYLGLYCEROL ACYLTRANSFERASE 2 (DGAT2) FAMILY MEMBERS AND USES THEREFOR

Номер: US20130078239A1
Автор: Gimeno Ruth E., Hubbard Brian K., Kapeller-Libermann Rosana, Wu Zhidan
Принадлежит: Millennium Pharmaceuticals, Inc.

The present invention relates to compositions and methods for the diagnosis and treatment of obesity and related metabolic disorders. The invention provides isolated nucleic acids molecules, designated DGAT2 family member nucleic acid molecules, which encode diacylglycerol acyltransferase family members. The invention also provides recombinant expression vectors containing DGAT2 family member nucleic acid molecules, host cells into which the expression vectors have been introduced, and nonhuman transgenic animals in which a DGAT2 family member gene has been introduced or disrupted. The invention still further provides isolated DGAT2 family member proteins, fusion proteins, antigenic peptides and anti-DGAT2 family member antibodies. Methods of use of the provided DGAT2 family member compositions for screening, diagnostic and therapeutic methods in connection with obesity disorders are also disclosed. 1. An isolated nucleic acid molecule selected from the group consisting of:a) a nucleic acid molecule comprising a nucleotide sequence which is at least 85% identical to the nucleotide sequence of SEQ ID NO:7, SEQ ID NO:19, or SEQ ID NO:61;b) a nucleic acid molecule comprising a fragment of at least 300 nucleotides of the nucleotide sequence of SEQ ID NO:7, SEQ ID NO:19 or SEQ ID NO:61;c) a nucleic acid molecule which encodes a polypeptide comprising the amino acid sequence selected from the group consisting of SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:12, SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:20 and SEQ ID NO:62;d) a nucleic acid molecule which encodes a fragment of a polypeptide comprising the amino acid sequence of SEQ ID NO:8, SEQ ID NO:20, or SEQ ID NO:62, wherein the fragment comprises at least 15 contiguous amino acids of SEQ ID NO:8, SEQ ID NO:20 or SEQ ID NO:62;e) a nucleic acid molecule which encodes a naturally occurring allelic variant of a polypeptide comprising the amino acid sequence of SEQ ID NO:8, SEQ ID NO:20 or SEQ ID ...

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Номер записи: 24
18-04-2013 дата публикации

Acyltransferases and Uses Thereof in Fatty Acid Production

Номер: US20130097733A1
Автор: Haslam Richard, Lindberg Yilmaz Jenny, Marty Laurent, Napier Johnathan A., Noemi Ruiz Lopez, Sayanova Olga, Senger Toralf, Stymne Sten
Принадлежит: BASF Plant Science Company GmbH

The present invention relates to the recombinant manufacture of polyunsaturated fatty acids. Specifically, it relates to acyltransferase polypeptides, polynucleotides encoding said acyltransferases as well as vectors, host cells, non-human transgenic organisms containing said polynucletides. Moreover, the present invention contemplates methods for the manufacture of polyunsaturated fatty acids as well as oils obtained by such methods. 1. A polynucleotide comprising a nucleic acid sequence elected selected from the group consisting of:a) the nucleic acid sequence of SEQ ID NOs: 52, 1, 4, 7, 10, 13, 16, 19, 22, 25, 28, 31, 34, 37, 40, 43, 46, 49, and 55;b) a nucleic acid sequence encoding the amino acid sequence of SEQ ID NO: 53, 2, 5, 8, 11, 14, 17, 20, 23, 26, 29, 32, 35, 38, 41, 44, 47, 50, or 56;c) a nucleic acid sequence being at least 40% identical to the nucleic acid sequence of a) or b), wherein said nucleic acid sequence encodes a polypeptide having acyltransferase activity;d) a nucleic acid sequence encoding a polypeptide having acyltransferase activity and having an amino acid sequence which is at least 45% identical to the amino acid sequence of b); and ["i) hybridization in 50 mM Tris, pH 7.6, 6×SSC, 5× Denhardt's, 1.0% sodium dodecyl sulfate (SDS), 100 μg denaturated calf thymus DNA at 34° C. overnight and wash twice with 2×SSC, 0.5% SDS at room temperature for 15 min each, repeat twice with 0.2×SSC, 0.5% SDS at room temperature for 15 min each and then repeat twice with 0.2 SSC, 0.5% SDS at 50° C. for 15 min;", "ii) hybridization in 6×SSPE (Sodium chloride Sodium Phosphate-EDTA), 5× Denhardt's solution, 0.5% SDS, 100 μg denaturated calf thymus DNA at 34° C. overnight and wash twice with 2×SSC, 0.5% SDS at room temperature for 15 min each, repeat twice with 0.2×SSC, 0.5% SDS at room temperature for 15 min each and then repeat twice with 0.2 SSC, 0.5% SDS at 50° C. for 15 min;", "iii) hybridization in 20-30% formamide, 5×SSPE, 5× Denhardt's solution, 1% ...

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Номер записи: 25
25-04-2013 дата публикации

Novel glyphosate-n-acetyltransferase (gat) genes

Номер: US20130102765A9
Автор: Billy Fred Mccutchen, Christina Ivy, Dan Siehl, Jeremy Minshull, Linda A. Castle, Lorraine Giver, Nicholas B. Duck, Phillip A. Patten, Rebecca Gorton, Roger Kemble, Yong Hong Chen
Принадлежит: EI Du Pont de Nemours and Co, PIONEER HI BRED INTERNATIONAL INC, Verdia LLC

Novel proteins are provided herein, including proteins capable of catalyzing the acetylation of glyphosate and other structurally related proteins. Also provided are novel polynucleotides capable of encoding these proteins, compositions that include one or more of these novel proteins and/or polynucleotides, recombinant cells and transgenic plants comprising these novel compounds, diversification methods involving the novel compounds, and methods of using the compounds. Some of the novel methods and compounds provided herein can be used to render an organism, such as a plant, resistant to glyphosate.

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Номер записи: 26
02-05-2013 дата публикации

PROTEIN EXHIBITING ACTIVITY OF PYRETHRIN BIOSYNTHETIC ENZYME, GENE ENCODING THE PROTEIN, AND VECTOR BEARING THE GENE

Номер: US20130109076A1
Автор: Kikuta Yukio, Matsuda Kazuhiko
Принадлежит:

Amino acid sequences of an enzyme involved in pyrethrin biosynthesis and a base sequence of the gene thereof; constructing vectors bearing the gene and transformants; and extractable from plant bodies producing pyrethrin by applying such creative techniques to plant bodies with faster growth aiming to provide a method to efficiently produce pyrethrin. A gene encoding a protein consisting of the amino acid sequence of SEQ ID NO: 1. A protein consisting of the amino acid sequence of SEQ ID NO: 2. 1. A pyrethrin biosynthetic enzyme , produced by a method comprising the sequential steps of:obtaining a raw material from a pyrethrum flower;obtaining from the raw material a precipitate of a crude protein fractionation with ammonium sulfate precipitation;crudely purifying the precipitate by a batch method using a hydrophobic resin;purifying the enzyme solution obtained by crude purification with anion-exchange chromatography;purifying with hydrophobic chromatography;purifying with gel filtration to obtain an enzyme protein with a molecular weight of approximately 40,000; andtransforming an initial part of the enzyme protein into maltose binding protein sequence, wherein the transformed enzyme protein has a molecular weight of approximately 80,000.2. A protein consisting of the amino acid sequence set forth in SEQ ID NO: 2.3. A gene encoding a protein consisting of the amino acid sequence set forth in SEQ ID NO: 2.4. A vector comprising the gene according to . This is a Continuation-in-Part of application Ser. No. 13/137,327 filed Aug. 5, 2011, which in turn is a Continuation-in-Part of application Ser. No. 12/457,193 filed Jun. 3, 2009, and claims the benefit of Japanese Application No. 2008-208295 filed Aug. 13, 2008. The disclosure of the prior applications is hereby incorporated by reference herein in its entirety.The present invention relates to a protein exhibiting activity of a pyrethrin biosynthetic enzyme, a gene encoding thereof, and vector bearing the gene. ...

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Номер записи: 27
09-05-2013 дата публикации

METHOD TO PRODUCE ACETYLDIACYLGLYCEROLS (AC-TAGS) BY EXPRESSION OF AN ACETYLTRANSFERASE GENE ISOLATED FROM EUONYMUS ALATUS (BURNING BUSH)

Номер: US20130116462A1
Автор: Durrett Timothy, Ohlrogge John, Pollard Michael
Принадлежит: BOARD OF TRUSTEES OF MICHIGAN STATE UNIVERSITY

The present invention relates to novel diacylglycerol acyltransferase genes and proteins, and methods of their use. In particular, the invention describes genes encoding proteins having diacylglycerol acetyltransferase activity, specifically for transferring an acetyl group to a diacylglycerol substrate to form acetyl-Triacylglycerols (ac-TAGS), for example, a 3-acetyl-1,2-diacyl-sn-glycerol. The present invention encompasses both native and recombinant wild-type forms of the transferase, as well as mutants and variant forms. The present invention also relates to methods of using novel diacylglycerol acyltransferase genes and proteins, including their expression in transgenic organisms at commercially viable levels, for increasing production of 3-acetyl-1,2-diacyl-sn-glycerols in plant oils and altering the composition of oils produced by microorganisms, such as yeast, by increasing ac-TAG production. Additionally, oils produced by methods of the present inventions comprising genes and proteins are contemplated for use as biodiesel fuel, in polymer production and as naturally produced food oils with reduced calories. 1. An isolated nucleic acid sequence encoding a short chain acyl-CoA diacylglycerol acyltransferase plant protein.2. The nucleic acid sequence of claim 1 , wherein said protein is capable of acetylating a diacylglycerol substrate comprising a fatty acid to form a acetyltriacylglycerol.3. The nucleic acid sequence of claim 2 , wherein said fatty acid is selected from the group consisting of butyrate claim 2 , caproate claim 2 , caprylate claim 2 , caprate claim 2 , laurate claim 2 , myristate claim 2 , palmitate claim 2 , palmitoleate claim 2 , stearate claim 2 , oleate claim 2 , linoleate claim 2 , linolenate claim 2 , arachidonate claim 2 , eicosenoate claim 2 , eicosadienoate claim 2 , and erucate.4. The nucleic acid sequence of claim 2 , wherein said diacylglycerol substrate is selected from the group consisting of 1 claim 2 ,2-dipalmitoyl-glycerol ...

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Номер записи: 28
16-05-2013 дата публикации

Alpha-tubulin acetyltransferase

Номер: US20130121989A1
Автор: Dorota Wloga, Jacek Gaertig, Shilpa Akella
Принадлежит: University of Georgia Research Foundation Inc UGARF

Polypeptides with tubulin acetyltransferase activity are described, as are nucleic acids encoding said polypeptides, and methods of use. The invention further provides enhancers and inhibitors of tubulin acetyltransferase activity, as well as cells having altered tubulin transferase activity.

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Номер записи: 29
23-05-2013 дата публикации

VARIANT LOVD POLYPEPTIDES AND THEIR USES

Номер: US20130130351A1
Автор: ALVIZO Oscar, Collier Steven James, Gilson Lynne, SUKUMARAN Joly, Teo Ee Ling, Wilson Robert John, Xu Junye, YEO Wan Lin
Принадлежит: CODEXIS, INC.

The present disclosure provides acyltransferases useful for synthesizing therapeutically important statin compound 1. An isolated or recombinant polynucleotide encoding a variant LovD polypeptide having acyltransferase activity , which comprises the amino acid sequence of SEQ ID NO:2 with the mutations L174F and A178L and from 1 to 30 additional mutations.2. The polynucleotide of claim 1 , wherein the encoded LovD polypeptide amino acid sequence includes the following additional mutations: A123P claim 1 , N191S/G claim 1 , A247S and L361M.3. The polynucleotide of claim 1 , wherein the 1 to 30 additional mutations of the encoded LovD polypeptide amino acid sequence are selected from the group consisting of I4N claim 1 , A9V claim 1 , K26E claim 1 , R28K claim 1 , R285 claim 1 , 135L claim 1 , C40A claim 1 , C40V claim 1 , C40F claim 1 , C40R claim 1 , S41R claim 1 , N43R claim 1 , N43Y claim 1 , C60F claim 1 , C60R claim 1 , C60Y claim 1 , C60N claim 1 , C60H claim 1 , D96R claim 1 , S109C claim 1 , A123P claim 1 , S142N claim 1 , A184T claim 1 , A184V claim 1 , N191S/G claim 1 , Q241M claim 1 , A247S claim 1 , D254E claim 1 , A261H claim 1 , A261T claim 1 , A261E claim 1 , A261V claim 1 , L292R claim 1 , Q295R claim 1 , Q297E claim 1 , L335M claim 1 , L361M claim 1 , A377V claim 1 , A383V claim 1 , N391D claim 1 , H404K claim 1 , H404R claim 1 , Q412R.4. The polynucleotide of claim 1 , wherein the codons encoding the variant LovD polypeptide sequence have been optimized for expression in a host cell.5E. coli.. The polynucleotide of claim 1 , wherein the host cell is6. An expression vector comprising the polynucleotide of operably linked to a control sequence suitable for directing expression of the variant LovD polypeptide in a host cell.7. The expression vector of claim 6 , wherein the control sequence comprises a promoter.8. A host cell comprising a polynucleotide according to .9E. coli.. The host cell of claim 8 , wherein the host cell is10. A method of making a ...

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Номер записи: 30
13-06-2013 дата публикации

Marchantiales-derived unsaturated fatty acid synthetase genes and use of the same

Номер: US20130152229A1
Автор: Kanji Ohyama
Принадлежит: Suntory Holdings Ltd

A Δ5 fatty acid desaturase gene, a Δ6 fatty acid desaturase gene, and a Δ6 fatty-acid-chain elongase gene are isolated from a single species of Marchantiales. By introducing these genes into higher plants, transformed plants which can produce arachidonic acid and eicosapentaenoic acid (EPA) are obtained.

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Номер записи: 31
13-06-2013 дата публикации

GENES ENCODING A NOVEL TYPE OF LYSOPHOPHATIDYLCHOLINE ACYLTRANSFERASES AND THEIR USE TO INCREASE TRIACYLGLYCEROL PRODUCTION AND/OR MODIFY FATTY ACID COMPOSITION

Номер: US20130152230A1
Автор: Chen Qilin, Xu Jingyu, ZHENG Zhifu, Zou Jitao
Принадлежит:

Described nucleic acid molecules (and corresponding peptides) encode lyso-phosphatidylcholine (LPC) acyltransferases. Over-expression of the LPC acyltransferases in a cell may lead to enhanced production of PUFA, or other unusual fatty acids, and/or to increased oil content in the cell. 1. A nucleic acid molecule , wherein said nucleic molecule is isolated , purified or recombinant , and comprises the sequence of SEQ ID NO:1 , SEQ ID NO:3 , SEQ ID NO:5 , SEQ ID NO:7 , SEQ ID NO:9 , SEQ ID NO:12 , SEQ ID NO:14 , SEQ ID NO:16 , SEQ ID NO:18 , SEQ ID NO:20 , SEQ ID NO:24 , SEQ ID NO:26 , SEQ ID NO:28 , SEQ ID NO:30 , SEQ ID NO:32 , or SEQ ID NO:34.2. An isolated peptide encoded by the nucleic acid molecule of .3. An isolated peptide comprising an amino acid sequence selected from the group consisting of SEQ ID NO:2 claim 1 , SEQ ID NO:4 claim 1 , SEQ ID NO:6 claim 1 , SEQ ID NO:8 claim 1 , SEQ ID NO:10 claim 1 , SEQ ID NO:11; SEQ ID NO:13 claim 1 , SEQ ID NO:15 claim 1 , SEQ ID NO:17; SEQ ID NO:19 claim 1 , SEQ ID NO:21 claim 1 , SEQ ID NO:25 claim 1 , SEQ ID NO:27 claim 1 , SEQ ID NO:29 claim 1 , SEQ ID NO:31 claim 1 , SEQ ID NO:33 claim 1 , SEQ ID NO:35 claim 1 , and an amino acid sequence having at least 60% homology to any thereof.4. The isolated peptide of claim 2 , wherein the homology is at least 70% to the amino acid sequence.5. The isolated peptide of claim 2 , wherein the peptide consists of the amino acid sequence.6. A method for identifying a lyso-phosphatidylcholine acyltransferase claim 2 , the method comprising:screening a peptide or a nucleic acid sequence encoding the peptide for at least one motif selected from the group consisting of SEQ ID NO:46, SEQ ID NO:47, SEQ ID NO:48, SEQ ID NO:49, SEQ ID NO:81, SEQ ID NO:82, SEQ ID NO:83, and SEQ ID NO:84, or screening the corresponding nucleic acid sequence or sequences thereof.7. A method of identifying a peptide for lyso-phosphatidylcholine acyltransferase activity claim 2 , the method comprising:screening ...

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Номер записи: 32
20-06-2013 дата публикации

Mutant polyhydroxyalkanoic acid synthase gene and method for producing aliphatic polyester using the same

Номер: US20130157327A1
Автор: Hiromi Kambe, Masakazu Ito, Masayoshi Muramatsu
Принадлежит: Toyota Motor Corp

A substitution mutation that improves polymerization activity of a polyhydroxyalkanoic acid synthase is identified. At least 1 amino acid residue selected from the group consisting of a histidine residue at position 17, a proline residue at position 71, a valine residue at position 131, a methionine residue at position 205, a leucine residue at position 230, and a proline residue at position 239 of a polyhydroxyalkanoic acid synthase derived from Alcanivorax borkumensis is subjected to substitution mutation with another amino acid.

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Номер записи: 33
18-07-2013 дата публикации

Fermentive production of four carbon alcohols

Номер: US20130183731A1
Автор: Andrew C. Eliot, Dennis Flint, Gail K. Donaldson, Lori Ann Maggio-Hall, Vasantha Nagarajan
Принадлежит: BUTAMAX ADVANCED BIOFUELS LLC

Methods for the fermentative production of four carbon alcohols is provided. Specifically, butanol, preferably isobutanol is produced by the fermentative growth of a recombinant bacterium expressing an isobutanol biosynthetic pathway.

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Номер записи: 34
18-07-2013 дата публикации

Method of meristem excision and transformation

Номер: US20130185830A1
Автор: Anatoly Rivlin, Brian J. Martinell, Erik Dersch, Richard J. Heinzen, Xudong Ye, Yuechun Wan
Принадлежит: MONSANTO TECHNOLOGY LLC

The present invention relates to excision of explant material comprising meristematic tissue from cotton seeds. Methods for tissue preparation, storage, transformation, and selection or identification of transformed plants are disclosed, as are transformable meristem tissues and plants produced by such methods, and apparati for tissue preparation.

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Номер записи: 35
25-07-2013 дата публикации

MODIFIED TRANSKETOLASE AND USE THEREOF

Номер: US20130189731A1
Автор: Hans Michael, Hohmann Hans-Peter, Laudert Dietmar, LEHMANN Martin
Принадлежит: DSM IP ASSETS B.V.

The present invention relates to a improved process for the biotechnological production of compounds for which ribose-5-phosphate, ribulose-5-phosphate or xylulose-5-phosphate is biosynthetic precursor like riboflavin (vitamin B), FAD, FMN, pyridoxal phosphate (vitamin B), guanosine, GMP, adenosine, AMP. The invention further pertains to the generation of the organism producing those compounds. It furthermore relates to the generation of mutated transketolases that allow normal growth on glucose but reduced growth on gluconate when introduced into the production strains and to polynucleotides encoding them. 1. A modified transketolase , wherein the amino acid sequence of the modified transketolase contains at least one mutation , so that the specific activity of the modified enzyme is modulated in comparison to the corresponding non-modified wild-type enzyme.2Bacillus subtilis. A transketolase according to wherein the amino acid sequence of the modified transketolase contains a mutation at an amino acid position that corresponds to position 357 of the transketolase as shown in SEQ ID No. 2.3. A transketolase according to wherein the wild-type amino acid arginine is replaced by histidine claim 2 , alanine claim 2 , lysine claim 2 , serine claim 2 , threonine claim 2 , leucine claim 2 , valine claim 2 , isoleucine claim 2 , methionine claim 2 , glycine claim 2 , glutamine claim 2 , or asparagine.4. A transketolase according to wherein the wild-type amino acid arginine is replaced by histidine claim 2 , alanine claim 2 , lysine claim 2 , serine claim 2 , threonine claim 2 , asparagine claim 2 , or glycine.5Bacillus, Escherichia coli, Saccharomyces cerevisiae, Ashbya gossypii, Eremothecium ashbyiCorynebacterium glutamicum.. A transketolase according to which is a modification of the wild-type transketolase from the genus or6Bacillus.. A transketolase according to wherein the wild-type transketolase is from the genus7Bacillus subtilis.. A transketolase according to ...

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Номер записи: 36
08-08-2013 дата публикации

DGAT GENES FROM OLEAGINOUS ORGANISMS FOR INCREASED SEED STORAGE LIPID PRODUCTION AND ALTERED FATTY ACID PROFILES IN OILSEED PLANTS

Номер: US20130205444A1
Автор: DAMUDE HOWARD GLENN, MEYER KNUT, Ripp Kevin G., Stecca Kevin L.
Принадлежит: E.I. Du Pont De Nemours and Company

Transgenic soybean seed having increased total fatty acid content of at least 10% and altered fatty acid profiles when compared to the total fatty acid content of non-transgenic, null segregant soybean seed are described. DGAT genes from oleaginous organisms are used to achieve the increase in seed storage lipids. 1. An isolated polynucleotide comprising:(a) a nucleotide sequence encoding a polypeptide having diacylglycerol acyltransferase activity wherein the polypeptide has at least 80% amino acid identity, based on the Clustal V method of alignment, when compared to an amino acid sequence as set forth in SEQ ID NOs:135, 136, 162, 176, 215, 234, 265, 272, 279, 299, 304, 306, 310, 312, 314, 316, 318, 320, 322, 351, or 363;(b) a nucleotide sequence encoding a polypeptide having diacylglycerol acyltransferase activity, wherein the nucleotide sequence has at least 80% sequence identity, based on the BLASTN method of alignment, when compared to a nucleotide sequence as set forth in SEQ ID NO: 133, 134, 161, 175, 214, 233, 264, 271, 278, 298, 301, 303, 305, 309, 311, 313, 315, 317, 319, 321, 350, or 352;(c) a nucleotide sequence encoding a polypeptide having diacylglycerol acyltransferase activity, wherein the nucleotide sequence hybridizes under stringent conditions to a nucleotide sequence as set forth in SEQ ID NO: 133, 134, 161, 175, 214, 233, 264, 271, 278, 298, 301, 303, 305, 309, 311, 313, 315, 317, 319, 321, 350, or 352; or(d) a complement of the nucleotide sequence of (a), (b) or (c), wherein the complement and the nucleotide sequence consist of the same number of nucleotides and are 100% complementary.2. The polypeptide of claim 1 , wherein the amino acid sequence of the polypeptide has at least 85% sequence identity claim 1 , based on the Clustal V method of alignment claim 1 , when compared to SEQ ID NO: 135 claim 1 , 136 claim 1 , 162 claim 1 , 176 claim 1 , 215 claim 1 , 234 claim 1 , 265 claim 1 , 272 claim 1 , 279 claim 1 , 299 claim 1 , 304 claim 1 , ...

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Номер записи: 37
22-08-2013 дата публикации

FERULOYL-CoA:MONOLIGNOL TRANSFERASE

Номер: US20130219547A1
Автор: Ralph John, Wilkerson Curtis, Withers Saunia
Принадлежит: BOARD OF TRUSTEES OF MICHIGAN STATE UNIVERSITY

The invention relates to nucleic acids encoding a feruloyl-CoA:monolignol transferase and the feruloyl-CoA:monolignol transferase enzyme that enables incorporation of monolignol ferulates, for example, including p-coumaryl ferulate, coniferyl ferulate, and sinapyl ferulate, into the lignin of plants. 1. An isolated nucleic acid encoding a feruloyl-CoA:monolignol transferase wherein the nucleic acid can selectively hybridize to a DNA with a SEQ ID NO:1 sequence or the nucleic acid has at least about 90% sequence identity with SEQ ID NO:1.2. (canceled)3. (canceled)4. (canceled)5. The isolated nucleic acid of claim 1 , wherein the nucleic acid encodes a feruloyl-CoA:monolignol transferase that can catalyze the synthesis of monolignol ferulate(s) from monolignol(s) and feruloyl-CoA.6. (canceled)7. The isolated nucleic acid of claim 1 , wherein the nucleic acid encodes a feruloyl-CoA:monolignol transferase polypeptide with a SEQ ID NO:2 sequence.8. The isolated nucleic acid of claim 1 , wherein the nucleic acid encodes a feruloyl-CoA:monolignol transferase that can catalyze the synthesis of monolignol ferulate(s) from a monolignol(s) and feruloyl-CoA with at least about 50% claim 1 , of the activity of a feruloyl-CoA:monolignol transferase with the SEQ ID NO:2.9. A transgenic plant cell comprising the isolated nucleic acid of .10. A transgenic plant comprising the isolated nucleic acid of any of .11. An expression cassette comprising the feruloyl-CoA:monolignol transferase nucleic acid of operably linked to a promoter functional in a host cell.12. (canceled)13. (canceled)14. The expression cassette of claim 11 , wherein the expression cassette is within an expression vector.15. The expression cassette of claim 11 , wherein the promoter is a promoter functional during plant development or growth.16. The expression cassette of claim 11 , wherein the promoter is a poplar xylem-specific secondary cell wall specific cellulose synthase 8 promoter claim 11 , cauliflower mosaic ...

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Номер записи: 38
29-08-2013 дата публикации

Recombinant therapeutic glycine n-acyltransferase

Номер: US20130224175A1
Автор: Alberdina Aike Van Dijk, Christoffel Petrus Stephanus Badenhorst, Lodewyk Jacobus Mienie, Rencia Van Der Sluis
Принадлежит: NORTH WEST UNIVERSITY

This invention relates to a method of producing a recombinant enzyme, more particularly, this invention relates to a method of producing water soluble enzymatically active recombinant glycine N-acyltransferase (GLYAT (E.G. 2.1.3.13)), including the steps of providing a suitable expression host; preparing a vector including a gene for expressing GLYAT in the expression host to form an expression piasmid; transforming the host with the expression piasmid to form an expression system; expressing the GLYAT gene in the expression system; and separating the expressed GLYAT from the expression system.

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Номер записи: 39
29-08-2013 дата публикации

Recombinant microorganisms and uses therefor

Номер: US20130224838A1
Автор: Fungmin Liew, Michael Koepke, Sean Simpson, Wendy Chen
Принадлежит: Individual

The invention provides, inter alia, methods for the production of acetone, isopropanol and/or precursors of acetone and/or isopropanol by microbial fermentation of substrates comprising CO, genetically modified microorganisms of use in such methods, nucleic acids suitable for preparation of genetically modified microorganisms, a novel alcohol dehydrogenase and nucleic acids encoding same.

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Номер записи: 40
29-08-2013 дата публикации

NOVEL DGAT GENES FOR INCREASED SEED STORAGE LIPID PRODUCTION AND ALTERED FATTY ACID PROFILES IN OILSEED PLANTS

Номер: US20130225799A1
Автор: BERMUDEZ ERICKA, DAMUDE HOWARD GLENN, LI CHANGJIANG, MEYER KNUT, ROESLER KEITH, SHEN BO, TARCZYNSKI MITCHELL C.
Принадлежит:

Transgenic oilseeds having increased total fatty acid content of at least 10% and altered fatty acid profiles when compared to the total fatty acid content of null segregant oilseeds are described. Novel DGAT genes are used to achieve the increase in seed storage lipids. 1(a) a nucleotide sequence encoding a polypeptide having diacylglycerol acyltransferase activity wherein the polypeptide has at least 80% amino acid identity, based on the Clustal V method of alignment, when compared to an amino acid sequence as set forth in SEQ ID NOs:8, 10, or 12;(b) a nucleotide sequence encoding a polypeptide having diacylglycerol acyltransferase activity, wherein the nucleotide sequence has at least 80% sequence identity, based on the BLASTN method of alignment, when compared to a nucleotide sequence as set forth in SEQ ID NO: 7, 9, or 11:(c) a nucleotide sequence encoding a polypeptide having diacylglycerol acyltransferase activity, wherein the nucleotide sequence hybridizes under stringent conditions to a nucleotide sequence as set forth in SEQ ID NO: 7, 9, or 11; or(d) a complement of the nucleotide sequence of (a), (b) or (c), wherein the complement and the nucleotide sequence consist of the same number of nucleotides and are 100% complementary.. An isolated polynucleotide comprising: This application is a continuation of U.S. application Ser. No. 13/329,939, filed Dec. 19, 2011, which is a continuation of U.S. application Ser. No. 12/470,569, now U.S. Pat. No. 8,101,819, filed May 22, 2009, which claims the benefit of U.S. Provisional Application No. 61/055,579, filed May 23, 2008, the contents of which are hereby incorporated by reference.This invention is in the field of biotechnology, in particular, this pertains to polynucleotide sequences encoding diacylglycerol acyltransferase genes and the use of these acyltransferases for increased seed storage lipid production and altered fatty acid profiles in oilseed plants.The official copy of the sequence listing is submitted ...

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Номер записи: 41
05-09-2013 дата публикации

Peroxisome biogenesis factor protein (pex) disruptions for altering polyunsaturated fatty acids and total lipid content in oleaginous eukaryotic organisms

Номер: US20130230891A1
Автор: Narendra S. Yadav, Pamela L. Sharpe, Quinn Qun Zhu, Seung-Pyo Hong, Zhixiong Xue
Принадлежит: EI Du Pont de Nemours and Co

Methods of increasing the amount of polyunsaturated fatty acids (PUFAs) in the total lipid fraction and in the oil fraction of PUFA-producing, oleaginous eukaryotes, accomplished by modifying the activity of peroxisome biogenesis factor (Pex) proteins. Disruptions of a chromosomal Pex3 gene, Pex10p gene or Pex16p gene in a PUFA-producing, oleaginous eukaryotic strain resulted in an increased amount of PUFAs, as a percent of total fatty acids and as a percent of dry cell weight, in the total lipid fraction and in the oil fraction of the strain, as compared to the parental strain whose native Pex protein was not disrupted.

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Номер записи: 42
26-09-2013 дата публикации

PLANT DIACYLGLYCEROL ACYLTRANSFERASES

Номер: US20130252253A1
Автор: Butler Karlene H, Cahoon Edgar Benjamin, Cahoon Rebecca E., Kinney Anthony J.
Принадлежит: E I DU PONT DE NEMOURS AND COMPANY

This invention relates to an isolated nucleic acid fragment encoding a diacylglycerol acyltransferase. The invention also relates to the construction of a chimeric gene encoding all or a portion of the diacylglycerol acyltransferase, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the diacylglycerol acyltransferase in a transformed host cell. 1. An isolated polynucleotide comprising a nucleotide sequence encoding a first polypeptide of at least 50 amino acids that has at least 60% identity based on the Clustal method of alignment when compared to a polypeptide selected from the group consisting of SEQ ID NOs:4 , 6 , 8 , 10 , 14 , 20 and 22 ,or an isolated polynucleotide comprising the complement of the nucleotide sequence.2. The isolated polynucleotide of claim 1 , wherein the isolated nucleotide sequence consists of a nucleic acid sequence selected from the group consisting of SEQ ID NOs:1 claim 1 , 7 claim 1 , 13 claim 1 , 15 claim 1 , and 21 that codes for the polypeptide selected from the group consisting of SEQ ID NOs:2 claim 1 , 8 claim 1 , 14 claim 1 , 16 claim 1 , and 22.3. The isolated polynucleotide of wherein the isolated polynucleotide is DNA.4. The isolated polynucleotide of wherein the isolated polynucleotide is RNA.5. A chimeric gene comprising the isolated polynucleotide of operably linked to suitable regulatory sequences.6. An isolated host cell comprising the chimeric gene of .7. An isolated host cell comprising the isolated polynucleotide of .8. The isolated host cell of wherein the isolated host is selected from the group consisting of yeast claim 7 , bacteria claim 7 , plant claim 7 , and virus.9. A virus comprising the isolated polynucleotide of .10. A method of selecting an isolated polynucleotide that affects the level of expression of a diacylglycerol acyltransferase polypeptide in a plant cell claim 1 , the method comprising the steps of:(a) constructing an isolated ...

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Номер записи: 43
26-09-2013 дата публикации

GLYCEROL-3-PHOSPHATE ACYL TRANSFERASE

Номер: US20130252308A1
Автор: Ochiai Misa
Принадлежит: SUNTORY HOLDINGS LIMITED

The present invention relates to glycerol-3-phosphate acyltransferases, polynucleotides encoding the same, etc. The present invention provides a polynucleotide comprising the nucleotide sequence of e.g., SEQ ID NO: 1 or 4, a polynucleotide encoding a protein consisting of the amino acid sequence of SEQ ID NO: 2, an expression vector and transformant comprising the polynucleotide, a method for producing food, etc. using the transformant, food, etc. produced by the method, and so on. 1. A polynucleotide of any one selected from the group consisting of (a) to (e) below:(a) a polynucleotide comprising the nucleotide sequence of SEQ ID NO: 1 or 4;(b) a polynucleotide encoding a protein consisting of the amino acid sequence of SEQ ID NO: 2;(c) a polynucleotide encoding a protein consisting of an amino acid sequence wherein 1 to 100 amino acids are deleted, substituted, inserted and/or added in the amino acid sequence of SEQ ID NO: 2, and having a glycerol-3-phosphate acyltransferase activity;(d) a polynucleotide encoding a protein having an amino acid sequence having at least 85% homology to the amino acid sequence of SEQ ID NO: 2, and having a glycerol-3-phosphate acyltransferase activity; and,(e) a polynucleotide which hybridizes to a polynucleotide consisting of a nucleotide sequence complementary to the nucleotide sequence of SEQ ID NO: 1 or 4 under stringent conditions, and which encodes a protein having aglycerol-3-phosphate acyltransferase activity.2. The polynucleotide according to of any one as defined in (f) or (g) below:(f) a polynucleotide encoding a protein consisting of an amino acid sequence wherein 1 to 10 amino acids are deleted, substituted, inserted and/or added in the amino acid sequence of SEQ ID NO: 2, and having a glycerol-3-phosphate acyltransferase activity; and,(g) a polynucleotide encoding a protein having an amino acid sequence having at least 90% homology to the amino acid sequence of SEQ ID NO: 2, and having a glycerol-3-phosphate ...

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Номер записи: 44
10-10-2013 дата публикации

PRODUCING ALPHA-OLEFINS USING POLYKETIDE SYNTHASES

Номер: US20130267696A1
Автор: Fortman Jeffrey L., Katz Leonard, Keasling Jay D., Steen Eric J.
Принадлежит: The Regents of the University of California

The present invention provides for a polyketide synthase (PKS) capable of synthesizing an α-olefin, such as 1-hexene or butadiene. The present invention also provides for a host cell comprising the PKS and when cultured produces the α-olefin. 1. A non-naturally occurring polyketide synthase (PKS) , or functional variant thereof , capable of synthesizing an α-olefin.2. The PKS of claim 1 , wherein the α-olefin is not a compound synthesized by a naturally occurring PKS.3. The PKS of claim 1 , wherein the PKS is a hybrid PKS comprising modules claim 1 , domains claim 1 , and/or portions thereof claim 1 , or functional variant thereof claim 1 , from two or more PKSs.4Lyngbya majuscula. The PKS of claim 3 , wherein the PKS comprises a terminal module comprising ST claim 3 , and TE claim 3 , or functional variant thereof claim 3 , of CurM.5Lyngbya majuscula. The PKS of claim 4 , wherein the PKS comprises a terminal module comprising KR claim 4 , ACP claim 4 , ST claim 4 , and TE claim 4 , or functional variant thereof claim 4 , of CurM.6Lyngbya majuscula. The PKS of claim 5 , wherein the PKS comprises a terminal module comprising AT claim 5 , KR claim 5 , ACP claim 5 , ST claim 5 , and TE claim 5 , or functional variant thereof claim 5 , of CurM.7. The PKS of claim 3 , wherein the PKS comprises at least one terminal module comprising a ST and a TE claim 3 , or functional variant thereof claim 3 , described in Tables 2-4.8. The PKS of claim 3 , wherein the PKS comprises a loading module which incorporates acrylyl-CoA.9. The PKS of claim 3 , wherein the PKS comprises the loading module is a DEBS proprionyl-CoA specific loading domain modified to accept acrylyl-CoA.10. The PKS of claim 9 , wherein the PKS comprises a module in which a terminal carbon is incorporated as a methyl group which is later oxidized.12. The PKS of claim 11 , wherein the α-olefin is 1-hexene claim 11 , 1-decene claim 11 , or (E)-deca-1 claim 11 ,5-diene.15. A recombinant nucleic acid encoding the ...

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Номер записи: 45
17-10-2013 дата публикации

METHODS FOR TREATING ATHEROSCLEROSIS

Номер: US20130273024A1
Автор: KAYSER Frank, Labelle Marc, Shan Bei, Zhang Jian, Zhou Mingyue
Принадлежит:

The invention provides compounds, pharmaceutical compositions and methods for treating atherosclerosis, inflammation, thrombosis and other conditions and for decreasing or prevention of accumulation of cholesterol in a subject by modifying LCAT polypeptide. 2. The method of claim 1 , wherein X and Y are each —N═.3. The method of claim 2 , wherein Z is —S—.4. The method of claim 1 , wherein L is —S—.5. The method of claim 1 , wherein Ris CN.6. The method of claim 1 , wherein Ris SR.7. The method of claim 1 , wherein Ris C-Calkyl.8. The method of claim 7 , wherein Ris methyl.9. The method of claim 1 , wherein the compound is selected from the group consisting of3-(5-(Methylthio)-1,3,4-thiadiazol-2-ylthio)pyrazine-2-carbonitrile3-(5-(Ethylthio)-1,3,4-thiadiazol-2-ylthio)pyrazine-2-carbonitrile3-(5-(Allylthio)-1,3,4-thiadiazol-2-ylthio)pyrazine-2-carbonitrile3-(5-(Propylthio)-1,3,4-thiadiazol-2-ylthio)pyrazine-2-carbonitrile3-(5-(Butylthio)-1,3,4-thiadiazol-2-ylthio)pyrazine-2-carbonitrile3-(5-(Isobutylthio)-1,3,4-thiadiazol-2-ylthio)pyrazine-2-carbonitrile3-(5-(Pentylthio)-1,3,4-thiadiazol-2-ylthio)pyrazine-2-carbonitrile3-(5-(Dodecylthio)-1,3,4-thiadiazol-2-ylthio)pyrazine-2-carbonitrile3-(5-(Benzylthio)-1,3,4-thiadiazol-2-ylthio)pyrazine-2-carbonitrile3-(5-Mercapto-1,3,4-thiadiazol-2-ylthio)pyrazine-2-carbonitrile3-(5-(Isopropylthio)-4-methyl-4H-1,2,4-triazol-3-ylthio)pyrazine-2-carbonitrile3-(5-(Methylthio)-1,2,4-thiadiazol-3-ylthio)pyrazine-2-carbonitrile3-(5-Methyl-1,3,4-thiadiazol-2-ylthio)pyrazine-2-carbonitrile3-(5-Butyl-1,3,4-thiadiazol-2-ylthio)pyrazine-2-carbonitrile3-(4-Methyl-4H-1,2,4-triazol-3-ylthio)pyrazine-2-carbonitrile3-(1-Methyl-1H-imidazol-2-ylthio)pyrazine-2-carbonitrile, and 'or a pharmaceutically acceptable salt thereof.', '2-Chloro-3-(5-(methylthio)-1,3,4-thiadiazol-2-ylthio)pyrazine'}10. A method for treating atherosclerosis in a subject in need thereof claim 1 , comprising administering a therapeutically effective amount of a modified LCAT ...

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Номер записи: 46
17-10-2013 дата публикации

MODIFIED POLYPEPTIDE HAVING HOMOSERINE ACETYLTRANSFERASE ACTIVITY AND MICROORGANISM EXPRESSING THE SAME

Номер: US20130273615A1
Автор: Heo In Kyung, Kim Hyun Ah, Kim Ju Eun, Kim So Young, Lee Han Jin, Na Kwang Ho, Seo Chang Il, Shin Yong Uk, Son Sung Kwang
Принадлежит: CJ CHEILJEDANG CORPORATION

The present invention relates to a polypeptide that is modified to have homoserine O-acetyltransferase activity, and in particular, the present invention provides a modified polypeptide having homoserine O-acetyltransferase activity, in which the amino acid at position 111 of a polypeptide having homoserine succinyltransferase activity is substituted with other amino acid. 1. A modified polypeptide having homoserine O-acetyltransferase activity having the amino acid sequence of SEQ ID NO: 17 or at least 95% homologous thereto , in which the amino acid at position 111 from the start amino acid methionine , of the sequence is substituted with glutamic acid.2. The modified polypeptide according to claim 1 , wherein the amino acid at position 112 of the polypeptide is further substituted with threonine or histidine.3. The modified polypeptide according to claim 1 , wherein the modified polypeptide has amino acid sequence of SEQ ID NO: 18.4. The modified polypeptide according to claim 1 , wherein the modified polypeptide exhibits resistance to feedback regulation by methionine claim 1 , through substitution of amino acids.5. The modified polypeptide according to claim 4 , wherein the amino acid is substituted with proline at position 29 claim 4 , substituted with glycine at position 114 claim 4 , substituted with serine at position 140 claim 4 , or one or more combinations of them.6. The modified polypeptide according to claim 5 , wherein the amino acid at position 112 of the polypeptide is further substituted with threonine or histidine.7. The modified polypeptide according to claim 5 , wherein the modified polypeptide has the amino acid sequence of SEQ ID NO: 21.8. A polynucleotide encoding the modified polypeptide of .9. The polynucleotide according to claim 8 , wherein the polynucleotide has any one of the nucleotide sequences of SEQ ID NOs: 24 to 29.10. A recombinant vector comprising polynucleotide sequences operably linked to the polynucleotide of .11. A ...

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Номер записи: 47
17-10-2013 дата публикации

1-deoxy-d-xylulose 5-phosphate synthase alleles responsible for enhanced terpene biosynthesis

Номер: US20130276166A1
Автор: Didier Merdinoglu, Eric Duchene, Phlippe Hugueney
Принадлежит: Genoplante Valor SAS

A method of enhancement of the 1-deoxy-D-xylulose 5-phosphate synthase (DXS) activity of plants or bacteria to increase terpenes production in cells, an enhanced DXS sequence likely to be obtained by this method, a method of enhancement of production of terpenes in a host cell containing the enhanced DXS enzyme, and transgenic bacterium or plants that express this polypeptide are described.

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Номер записи: 48
31-10-2013 дата публикации

COMPOSITIONS AND METHODS FOR MODULATING THE SENSITIVITY OF CELL TO AHAS INHIBITORS

Номер: US20130288377A1
Автор: Champagne Michele M., Urano Jun
Принадлежит: Synthetic Genomics, Inc.

Methods and materials useful for modulating the sensitivity of cells to an inhibitor of acetohydroxyacid synthase (AHAS) are disclosed. For example, nucleic acid molecules encoding AHAS large subunits are disclosed as well as methods for using such nucleic acid molecules to transform microbial cells and plant cells, and to confer modulated sensitivity to AHAS-inhibiting compounds onto such cells. Further provided are materials and methods useful for modulating growth, development, activity, and characteristics of host cells and organisms. 1. An isolated nucleic acid molecule comprising:(a) a nucleic acid sequence hybridizing under high stringency conditions to a nucleic acid sequence selected from the group consisting of SEQ ID NO: 2, SEQ ID NO: 4, and SEQ ID NO: 6, a complement thereof or a fragment of either; or(b) a nucleic acid sequence exhibiting 70% or greater identity to a nucleic acid sequence selected from the group consisting of SEQ ID NO: 2, SEQ ID NO: 4, and SEQ ID NO: 6, a complement thereof or a fragment of either; or(c) a nucleic acid sequence encoding a polypeptide exhibiting 50% or greater identity to an amino acid sequence selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 3, and SEQ ID NO: 5; or(d) a nucleic acid sequence that is an interfering RNA to a nucleic acid sequence according to any one of paragraphs (a), (b), or (c).2. An isolated polypeptide claim 1 , wherein said polypeptide is encoded by a nucleic acid molecule according to .3. A nucleic acid molecule according to claim 1 , wherein said nucleic acid sequence encodes an acetohydroxyacid synthase.4Nannochloropsis. A nucleic acid molecule according to claim 1 , wherein said nucleic acid sequence encodes a acetohydroxyacid synthase.5. A nucleic acid molecule according to claim 1 , wherein said nucleic acid molecule encodes an acetohydroxyacid synthase having a reduced sensitivity to an AHAS inhibitor.6. A nucleic acid molecule according to claim 1 , wherein said nucleic acid ...

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Номер записи: 49
14-11-2013 дата публикации

Microorganisms and methods for the biosynthesis of adipate, hexamethylenediamine and 6-aminocaproic acid

Номер: US20130303723A1
Автор: Anthony P. Burgard, Mark J. Burk, Priti Pharkya, Robin E. Osterhout
Принадлежит: Genomatica Inc

The invention provides a non-naturally occurring microbial organism having a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in the respective 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The invention additionally provides a method for producing 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid. The method can include culturing a 6-aminocaproic acid, caprolactam or hexametheylenediamine producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway enzyme in a sufficient amount to produce the respective product, under conditions and for a sufficient period of time to produce 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid.

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Номер записи: 50
19-12-2013 дата публикации

PRODUCTION OF POLYUNSATURATED FATTY ACIDS BY COEXPRESSION OF ACYL-CoA:LYSOPHOSPHATIDYLCHOLINE ACYLTRANSFERASES AND PHOSPHOLIPID:DIACYLGLYCEROL ACYLTRANSFERASES

Номер: US20130337514A1
Автор: Hongxiang Zhang, Michael W. Bostick, Narendra S. Yadav, Quinn Qun Zhu
Принадлежит: EI Du Pont de Nemours and Co

Acyl-CoA:lysophosphatidylcholine acyltransferase [“LPCAT”] having the ability to convert acyl-CoA+1-acyl-sn-glycero-3-phosphocholine to CoA+1,2-diacyl-sn-glycero-3-phosphocholine (EC 2.3.1.23) is disclosed herein to be over-expressed along with the over-expression of phospholipid:diacylglycerol acyltransferase [“PDAT”] having the ability to transfer a fatty acyl group from the sn-2 position of a phospholipid (e.g., phosphatidylcholine) to the sn-3 position of 1,2-diacylglycerol [E.C.2.3.1.158], thus resulting in a lysophospholipid and TAG. Co-expression of these enzymes in a recombinant microbial host cell resulted in increased production of long chain polyunsaturated fatty acids [“PUFAs”].

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Номер записи: 51
19-12-2013 дата публикации

MUTANT ACYL-CoA:LYSOPHOSPHATIDYLCHOLINE ACYLTRANSFERASES

Номер: US20130337538A1
Автор: Hongxiang Zhang, Michael W. Bostick, Narendra S. Yadav, Quinn Qun Zhu
Принадлежит: EI Du Pont de Nemours and Co

Mutant acyl-CoA:lysophosphatidylcholine acyltransferases [“LPCATs”] having the ability to convert acyl-CoA+1-acyl-sn-glycero-3-phosphocholine to CoA+1,2-diacyl-sn-glycero-3-phosphocholine (EC 2.3.1.23) are disclosed herein. Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding mutant LPCATs, along with a method of making long chain polyunsaturated fatty acids [“PUFAs”] using these mutant LPCATs in oleaginous yeast, are also disclosed.

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Номер записи: 52
19-12-2013 дата публикации

Lipid comprising polyunsaturated fatty acids

Номер: US20130338388A1
Автор: James Robertson Petrie, Robert Charles de Feyter, Surinder Pal Singh
Принадлежит: Commonwealth Scientific and Industrial Research Organization CSIRO, Grains Research and Development Corp, Nuseed Pty Ltd

The present invention relates to extracted plant lipid, comprising fatty acids in an esterified form.

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Номер записи: 53
26-12-2013 дата публикации

Recombinant microorganisms make biodiesel

Номер: US20130344547A1
Автор: Fungmin Liew, Michael Koepke
Принадлежит: Lanzatech New Zealand Ltd

A carboxydotrophic acetogenic recombinant microorganism is modified so that it produces biodiesel and optionally one or more other products by fermentation of a substrate comprising CO. Biodiesel is produced by microbial fermentation of a substrate comprising CO. The recombinant microorganism is modified to express one or more exogenous enzymes in the biodiesel biosynthesis pathway not present in a parental microorganism from which the recombinant microorganism is derived. The one or more enzymes comprise a nonspecific acyltransferase.

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Номер записи: 54
09-01-2014 дата публикации

HUMAN DIACYLGLYCEROL ACYLTRANSFERASE 2 (DGAT2) FAMILY MEMBERS AND USES THEREFOR

Номер: US20140010799A1
Автор: Gimeno Ruth E., Hubbard Brian K., Kapellar-Libermann Rosana, Wu Zhidan
Принадлежит: Millenium Pharmaceuticals, Inc.

The present invention relates to compositions and methods for the diagnosis and treatment of obesity and related metabolic disorders. The invention provides isolated nucleic acids molecules, designated DGAT2 family member nucleic acid molecules, which encode diacylglycerol acyltransferase family members. The invention also provides recombinant expression vectors containing DGAT2 family member nucleic acid molecules, host cells into which the expression vectors have been introduced, and nonhuman transgenic animals in which a DGAT2 family member gene has been introduced or disrupted. The invention still further provides isolated DGAT2 family member proteins, fusion proteins, antigenic peptides and anti-DGAT2 family member antibodies. Methods of use of the provided DGAT2 family member compositions for screening, diagnostic and therapeutic methods in connection with obesity disorders are also disclosed. 1. An isolated nucleic acid molecule selected from the group consisting of:a) a nucleic acid molecule comprising a nucleotide sequence which is at least 85% identical to the nucleotide sequence of SEQ ID NO:7, SEQ ID NO:19, or SEQ ID NO:61;b) a nucleic acid molecule comprising a fragment of at least 300 nucleotides of the nucleotide sequence of SEQ ID NO:7, SEQ ID NO:19 or SEQ ID NO:61;c) a nucleic acid molecule which encodes a polypeptide comprising the amino acid sequence selected from the group consisting of SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:12, SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:20 and SEQ ID NO:62;d) a nucleic acid molecule which encodes a fragment of a polypeptide comprising the amino acid sequence of SEQ ID NO:8, SEQ ID NO:20, or SEQ ID NO:62, wherein the fragment comprises at least 15 contiguous amino acids of SEQ ID NO:8, SEQ ID NO:20 or SEQ ID NO:62;e) a nucleic acid molecule which encodes a naturally occurring allelic variant of a polypeptide comprising the amino acid sequence of SEQ ID NO:8, SEQ ID NO:20 or SEQ ID ...

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Номер записи: 55
09-01-2014 дата публикации

Microbial Synthesis Of Aldehydes And Corresponding Alcohols

Номер: US20140011231A1
Автор: Kwang Myung Cho, Shahrooz Rabizadeh, Wendy M. HIGASHIDE
Принадлежит: Easel Biotechnologies LLC

An improved process for alcohol production includes microbial fermentation using a genetically modified microorganism to produce substantial quantities of aldehydes that are stripped from the fermentation medium and condensed. So produced aldehydes are converted in an ex vivo process to corresponding alcohols.

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Номер записи: 56
30-01-2014 дата публикации

Producing Dicarboxylic Acids Using Polyketide Synthases

Номер: US20140030789A1
Автор: Jay D. Keasling, Jeffrey L. Fortman, Leonard Katz
Принадлежит: UNIVERSITY OF CALIFORNIA

The present invention provides for a polyketide synthase (PKS) capable of synthesizing a dicarboxylic acid (diacid). Such diacids include diketide-diacids and triketide-diacids. The invention includes recombinant nucleic acid encoding the PKS, and host cells comprising the PKS. The invention also includes methods for producing the diacids.

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Номер записи: 57
30-01-2014 дата публикации

LONG CHAIN OMEGA-3 AND OMEGA-6 POLYUNSATURATED FATTY ACID BIOSYNTHESIS BY EXPRESSION OF ACYL-CoA LYSOPHOSPHOLIPID ACYLTRANSFERASES

Номер: US20140031572A1
Автор: Hongxiang Zhang, Narendra S. Yadav, Quinn Qun Zhu
Принадлежит: EI Du Pont de Nemours and Co

Methods for increasing C 18 to C 20 elongation conversion efficiency and/or Δ4 desaturation conversion efficiency in long-chain polyunsaturated fatty acid [“LC-PUFA”]-producing recombinant oleaginous microbial host cells are provided herein, based on over-expression of acyl-CoA:lysophospholipid acyltransferases [“LPLATs”] (e.g., Ale1, LPAAT, LPCAT). Production host cells and oils produced by the methods of the invention are also claimed.

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Номер записи: 58
20-02-2014 дата публикации

Fermentive Production of Four Carbon Alcohols

Номер: US20140051151A1
Автор: Andrew C. Eliot, Dennis Flint, Gail K. Donaldson, Lori Ann Maggio-Hall, Vasantha Nagarajan
Принадлежит: BUTAMAX ADVANCED BIOFUELS LLC

Methods for the fermentative production of four carbon alcohols is provided. Specifically, butanol, preferably isobutanol is produced by the fermentative growth of a recombinant bacterium expressing an isobutanol biosynthetic pathway.

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Номер записи: 59
27-02-2014 дата публикации

AGENT THAT MODULATES PHYSIOLOGICAL CONDITION OF PESTS, INVOLVED IN INSECT CHOLINE ACETYLTRANSFERASE ACTIVITY

Номер: US20140057334A1
Автор: Craen Marc Van De, Demey Bert, Nys Guy, Otsuki Junko, Roobrouck Annelies
Принадлежит: Sumitomo Chemical Company, Limited

The present invention provides an agent that modulates physiological condition of pests, wherein the agent has an ability to modulate the activity of an insect choline acetyltransferase; a method for assaying pesticidal activity of a test substance, which comprises measuring the activity of a choline acetyltransferase in a reaction system in which the choline acetyltransferase contacts with a test substance, and the like. 1. An isolated insect choline acetyltransferase comprising an amino acid sequence selected from the group consisting of:(a) the amino acid sequence of SEQ ID NO: 1;(b) an amino acid sequence with deletion, addition or substitution of one or more amino acids in the amino acid sequence of SEQ ID NO: 1, wherein said amino acid sequence has choline acetyltransferase activity;(c) an amino acid sequence that has sequence identity of 50% or more to the amino acid sequence of SEQ ID NO: 1, wherein said amino acid sequence has choline acetyltransferase activity;(d) an amino acid sequence that has sequence similarity of 75% or more to the amino acid sequence of SEQ ID NO: 1, wherein said amino acid sequence has choline acetyltransferase activity;(e) the amino acid sequence encoded by the nucleotide sequence of SEQ ID NO: 2 or 3;(f) an amino acid sequence encoded by a nucleotide sequence that has sequence identity of 50% or more to the nucleotide sequence of SEQ ID NO: 2 or 3, wherein said amino acid sequence has choline acetyltransferase activity;(g) an amino acid sequence encoded by a polynucleotide, wherein said polynucleotide hybridizes under a stringent condition to a polynucleotide comprising a nucleotide sequence complementary to the nucleotide sequence of SEQ ID NO: 2 or 3, wherein said amino acid sequence has choline acetyltransferase activity; and(h) an amino acid sequence of a cotton aphid choline acetyltransferase.2. An isolated insect choline acetyltransferase comprising an amino acid sequence selected from the group consisting of:(a) the amino ...

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Номер записи: 60
20-03-2014 дата публикации

Methods and compositions for preventing norleucine misincorporation into proteins

Номер: US20140081003A1
Автор: Karthik VEERAVALLI, Michael W. Laird
Принадлежит: Genentech Inc

The present invention relates to methods and compositions for preventing incorporation of norleucine into proteins during recombinant protein production in bacteria. The present invention also provides microorganism host cells and nucleic acid molecules for use with the methods and compositions provided herein.

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Номер записи: 61
03-01-2019 дата публикации

BIOFILMS, COMPONENTS AND METHODS OF USE TO REDUCE BIOFOULING AND CONTAMINATION

Номер: US20190000090A1
Автор: Kumar Manish, Wood Thammajun L., Wood Thomas K.
Принадлежит:

Biofilms are provided which are capable of regulating their own thickness, reducing contamination and preventing biofouling. Constructs are introduced into bacteria that comprise nucleic acid molecules encoding an autoinducer synthase polypeptide, a transcriptional regulator and a biofilm dispersal protein. Nucleic acid molecules may also be introduced which encode a nitric oxide synthase, an epoxide hydrolase, or both. Biofilms of the bacteria may be used to reduce biofouling and contamination of a surface. 1. A method of producing a living self-controlled biofilm of engineered bacteria cells on a surface , said method comprising: i. a nucleic acid molecule encoding an autoinducer synthase polypeptide;', 'ii. a nucleic acid molecule encoding a transcriptional regulator, capable of being activated by said autoinducer synthase polypeptide; and', 'iii. a nucleic acid molecule encoding a biofilm dispersal protein to produce said at least one engineered bacteria cell comprising said construct; and, 'a) producing at least one engineered bacteria cell by introducing into said at least one bacterial cell a quorum sensing nucleic acid construct, comprising,'}b) producing a self-controlled biofilm of said at least one engineered bacteria-cells, wherein said quorum sensing nucleic acid construct in response to activation of said transcriptional regulatory and production of said biofilm dispersal protein reduces the thickness of said self-controlled biofilm compared to biofilm not comprising said quorum sensing nucleic acid construct.24.-. (canceled)5. The method of claim 1 , further comprising introducing into said at least one bacterial cell a nucleic acid molecule encoding nitric oxide synthase.6. The method of claim 1 , further comprising introducing into said bacterial cell a nucleic acid molecule encoding epoxide hydrolase.78.-. (canceled)9. The method of claim 1 , wherein said thickness of said biofilm is at least six fold less than said biofilm not comprising said ...

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Номер записи: 62
02-01-2020 дата публикации

ATTENUATION OF NEUROPATHIC PAIN AFTER SPINAL CORD INJURY

Номер: US20200000775A1
Автор: Geddes James W., Yu Chen Guang
Принадлежит:

Methods for treating neuropathic pain caused by a traumatic spinal cord injury are described. A method comprising administering an effective amount of flubendazole, an α-tubulin acetylation inhibitor, an endosomal NR1 and pERK1/2 inhibitor, a mitochondrial cyclin b1 inhibitor, a microtubule destabilizing drug, or combinations thereof to the patient suffering from the traumatic spinal cord injury. Also described is a method for preventing neuropathic pain in a patient with a spinal cord injury at risk for developing neuropathic pain comprising administering administrating an effective amount of flubendazole to a patient with the spinal cord injury at risk of developing neuropathic pain. 1. A method of treating pain in a patient having a traumatic spinal cord injury , comprising:administering an effective amount of flubendazole, an α-tubulin acetylation inhibitor, an endosomal NR1 and pERK1/2 inhibitor, a mitochondrial cyclin b1 inhibitor, a microtubule destabilizing drug, or combinations thereof to the patient.2. The method of claim 1 , wherein the pain is neuropathic pain.3. The method of claim 1 , wherein the pain is caused by excitotoxic neural injury.4. The method of claim 1 , wherein the patient is at risk for developing neuropathic pain.5. The method of claim 4 , comprising administering an effective amount of flubendazole to the patient.6. The method of claim 5 , wherein the treatment comprises substantially preventing neuropathic pain in the patient. This application is related to U.S. Provisional Application Ser. No. 62/691,969 filed Jun. 29, 2018, the entire disclosure of which is incorporated herein by this reference.This invention was made with government support under grant number UL1TR000117 awarded by the National Institutes of Health. The government has certain rights in the invention.The present invention relates to a method of using flubendazole and related compounds for the treatment of pain.Neuropathic pain is a debilitating consequence of spinal ...

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Номер записи: 63
06-01-2022 дата публикации

XYLR Mutant For Improved Xylose Utilization Or Improved Co-Utilization Of Glucose And Xylose

Номер: US20220002356A1
Автор: CALLIHAN Isolde, DA COSTA Bernardo Moura Torres, FRYKMAN Scott Allen, PAYNE Stephen Thomas, VENKITESWARAN Sankaranarayanan, WONG Leland Ken
Принадлежит:

The disclosure relates to mutant gene(s) that confer upon microorganisms that express them an improved capacity to utilize xylose and improved capacity to co-utilize glucose and xylose thereby resulting in improved growth of the microorganism. Further encompassed are methods of producing fatty acids and fatty acid derivatives from cellulosic biomass, xylose, and/or a glucose/xylose mix by employing the host cells expressing the engineered XylR variants and compositions of biologically produced fatty acids and fatty acid derivatives. 1. A XylR protein variant , wherein the XylR protein variant has at least one mutation at a position selected from positions 83 , 88 , 89 , 112 , 120 , 141 , 145 , 146 , 147 , 150 , 154 , 155 , 247 , 270 , 280 , 286 , 289 , 295 , 305 , 306 , 313 , 333 , 336 , 337 , 351 , 364 , 365 , 372 , and 382 of SEQ ID NO: 1.2. A recombinant host cell comprising the XylR protein variant of .3. A method for increasing xylose utilization in a recombinant host cell claim 2 , the method comprising culturing in a culture medium comprising xylose claim 2 , the recombinant host cell of claim 2 , wherein expression of the XylR protein variant confers improved xylose utilization of the recombinant host cell in comparison to the xylose utilization of a host cell expressing SEQ ID NO: 1 when the cells are cultured in the presence of xylose.4. The method of claim 3 , wherein the method is used for preparing a fatty acid derivative claim 3 , the method comprising culturing in a culture medium comprising xylose claim 3 , a recombinant host cell which further comprises at least one heterologous fatty acid derivative biosynthetic enzyme.5. The method of claim 4 , wherein the fatty acid derivative is: a fatty acid ester and wherein at least one heterologous fatty acid derivative biosynthetic enzyme has ester synthase activity claim 4 , and optionally wherein at least one heterologous fatty acid derivative biosynthetic enzyme is a thioesterase; a ω-hydroxy fatty acid ...

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Номер записи: 64
06-01-2022 дата публикации

MODULATION OF FORMATE OXIDATION BY RECOMBINANT YEAST HOST CELL DURING FERMENTATION

Номер: US20220002661A1
Автор: Argyros Aaron, Barrett Trisha, Skinner Ryan
Принадлежит:

The present disclosure concerns recombinant yeast host cells having a first genetic modification for increasing formate production, when compared to a corresponding native yeast host cell as well as a source of formate dehydrogenase activity. The source of formate can be an internal source of formate dehydrogenase activity and/or the recombinant yeast host call can be supplemented by an external source of formate dehydrogenase activity. 1. A recombinant yeast host cell having (i) a first genetic modification for increasing formate production , when compared to a corresponding native yeast host cell and (ii) a source of formate dehydrogenase activity , wherein the source of formate dehydrogenase activity is:an internal source of formate dehydrogenase activity provided by a second genetic modification; and/oran external source of formate dehydrogenase activity provided by a further yeast host cell having a third genetic modification.2. The recombinant yeast host cell of claim 1 , wherein the first genetic modification comprises introducing one or more first heterologous nucleic acid molecule encoding one or more polypeptide having pyruvate formate lyase activity in the recombinant yeast host cell.3. The recombinant yeast host cell of claim 2 , wherein the one or more polypeptide having pyruvate formate lyase activity comprises PFLA claim 2 , PFLB or a combination thereof.4Bifidobacterium. The recombinant yeast host cell of or claim 2 , wherein the one or more polypeptide having pyruvate formate lyase activity is from sp.5Bifidobacterium adolescentis.. The recombinant yeast host cell of claim 4 , wherein the one or more polypeptide having pyruvate formate lyase activity is from6. The recombinant yeast host cell of claim 5 , wherein the one or more polypeptide having pyruvate formate lyase activity comprises the amino acid sequence of SEQ ID NO: 6 claim 5 , is a variant of the amino acid sequence of SEQ ID NO: 6 having pyruvate formate lyase activity or is a fragment of ...

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Номер записи: 65
06-01-2022 дата публикации

Bahd acyltransferases

Номер: US20220002744A1
Автор: Brian Fox, Craig Bingman, Emily Beebe, Faride Unda, Heather Mackay, Hoon Kim, John Ralph, Rebecca Anne Smith, Shawn Mansfield, Steven D. Karlen, Yaseen Mottiar
Принадлежит: University of British Columbia, WISCONSIN ALUMNI RESEARCH FOUNDATION

The invention is directed to BAHD acyltransferase enzymes, nucleic acids encoding BAHD acyltransferase enzymes, and inhibitory nucleic acids adapted to inhibit the expression and/or translation of BAHD acyltransferase RNA; expression cassettes, plant cells, and plants that have or encode such nucleic acids and enzymes; and methods of making and using such nucleic acids, enzymes, expression cassettes, cells, and plants.

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Номер записи: 66
06-01-2022 дата публикации

MICROBIAL CELLS AND METHODS FOR PRODUCING CANNABINOIDS

Номер: US20220002764A1
Автор: Chen Lu, KUMARAN Ajikumar Parayil, PHILIPPE Ryan A., SANTOS Christine Nicole S.
Принадлежит:

Enzymes involved in cannabinoid biosynthesis are recombinantly expressed in a host cell. The host cell may be a prokaryote (e.g. ) or a eukaryote (e.g. ). The enzymes include a heterologous cannabigerolic acid synthase as well as additional enzymes involved in the biosynthesis of cannabinoid precursors such as geranyl diphosphate, olivetol, olivetolic acid, divarin and/or divarinic acid. Methods are provided for producing C5-cannabinoids and/or C3-cannabinoids by fermentation of the recombinant host cell. Alternatively, cannabinoids can be produced by biotransformation of cannabinoid precursors in recombinant cells or by disrupted recombinant cells. 1. A microbial cell for producing one or more cannabinoids , the microbial cell expressing a cannabinoid biosynthetic pathway comprising a heterologous prenyltransferase enzyme having cannabigerolic acid synthase (CBGAS) or cannabigerovarinic acid synthase (CBGVAS) activity ,the microbial cell further comprising one or more modifications that increases carbon flux to geranyl diphosphate (GPP) and/or carbon flux to one or more of hexanoic acid, hexanoyl-CoA, butyric acid, butyryl-CoA, and/or acetyl-CoA; and/orthe microbial cell produces the cannabinoid from one or more fed precursors selected from olivetol, olivetolic acid, divarin, divarinic acid, hexanoic acid, butyric acid, hexanoyl-CoA, butyryl-CoA, or derivative thereof and/or GPP precursor.2. The microbial cell of claim 1 , wherein the CBGAS or CBGVAS enzyme comprises the amino acid sequence of SEQ ID NO: 60 claim 1 , or a derivative thereof.3. The microbial cell of claim 1 , wherein the CBGAS or CBGVAS comprises an amino acid sequence selected from SEQ ID NO: 60 to 94 claim 1 , or a derivative thereof.4. The microbial cell of claim 3 , wherein the CBGAS comprises an amino acid sequence selected from: SEQ ID NOs: 63 claim 3 , 74 claim 3 , 77 claim 3 , 84-91 claim 3 , 93 and a derivative thereof.5. The microbial cell of claim 4 , wherein the derivative comprises the ...

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Номер записи: 67
06-01-2022 дата публикации

Construction and Application of Engineered Strain of Escherichia Coli for Producing Malic Acid by Fixing CO2

Номер: US20220002766A1
Автор: Danlei MA, Guipeng HU, Jia Liu, Liming Liu, Qiuling Luo, Xiulai CHEN
Принадлежит: JIANGNAN UNIVERSITY

The disclosure discloses construction and application of an engineered strain of E. coli for producing malic acid by fixing CO2, and belongs to the field of fermentation. The engineered strain is obtained by performing genetic engineering transformation on Escherichia coli MG1655; the genetic engineering transformation includes knocking out a fumarate reductase gene, a fumarase gene, a lactate dehydrogenase gene and an alcohol dehydrogenase gene and freely overexpressing a formate dehydrogenase, an acetyl coenzyme A synthetase, an acylated acetaldehyde dehydrogenase, a formaldehyde lyase, a dihydroxyacetone kinase, a malic enzyme and a phosphite oxidoreductase to obtain a strain GH0407. The strain is used for producing malic acid by fermentation, anaerobic fermentation is performed for 72 hours with CO2 and glucose as a co-substrate, the production of malic acid reaches 39 g/L, the yield is 1.53 mol/mol, and accumulation of malic acid in the original strain is not achieved.

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Номер записи: 68
07-01-2016 дата публикации

Methods and Apparatus for Cell-Free Microfluidic-Assisted Biosynthesis

Номер: US20160002611A1
Автор: Gershenfeld Neil A., Mershin Andreas, Noireaux Vincent, Pelletier James Francis
Принадлежит:

A trans-disciplinary system for cell-free biosynthesis includes a cell-free transcription-translation (TX-TL) tool and modular, generalizable microfluidic architectures. Both components of the system are independently functional and are combinable into a cell-free biosynthesis platform. In the first component, modular plasmid libraries are used to program bacterial cell-free TX-TL systems. Each plasmid holds one gene or operon, and all the genes are controlled by the same promoter, so that the stoichiometry of enzyme synthesis is determined by the stoichiometry of plasmids in the reaction. In the second part, in order to facilitate high throughput mixing and matching of gene units from the modular plasmid libraries, a modular, reconfigurable, flexible, and scalable microfluidic architecture is employed. The microfluidic modules share common form factors and port/valve locations, so that a small set of module types, with multiple instances of each type interconnected in different geometries, allows simple reconfiguration to achieve different modes of operation. 1. A method for cell-free synthesis of a biosynthetic product , comprising the steps of: preparing a selected bacterial cell culture;', 'generating a cell extract from the bacterial cell culture;', 'combining the cell extract with amino acid and energy solutions to create a transcription-translation reaction buffer; and', 'separating reaction buffer aliquots by adding enzyme genes;, 'performing cell-free transcription-translation by the steps ofinfusing a microfluidic device with the reaction buffer aliquots, wherein the microfluidic device comprises one or more microfluidic modules configured for generating, controlling, and manipulating droplets of the reaction buffer aliquots;generating, controlling, and manipulating the droplets according to the configuration of the microfluidic device; andextracting droplets containing target molecules from the microfluidic device.2. The method of claim 1 , wherein the ...

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Номер записи: 69
07-01-2016 дата публикации

COMPOSITIONS AND METHODS FOR INCREASED ETHANOL PRODUCTION FROM BIOMASS

Номер: US20160002676A1
Автор: ARISTIDOU Aristos, JESSEN Holly J., Liao Hans, LUNDORFF Joshua, NEGRETE-RAYMOND Ana, SUOMINEN Pirkko, YI Jian
Принадлежит:

The present application discloses the identification of the novel xylose transporter genes KHT105 and RAG4, as well as the identification of a novel set of pentose phosphate pathway genes The present application further discloses a series of genetically modified yeast cells comprising various combinations of arabinose fermentation pathways, xylose fermentation pathways, pentose phosphate pathways, and/or xylose transporter genes, and methods of culturing these cells to produce ethanol in fermentation media containing xylose. 116-. (canceled)17. A genetically modified yeast cell comprising an active arabinose fermentation pathway , wherein said cell comprises one or more exogenous arabinose fermentation pathway genes selected from the group consisting of AI , RK , and RE genes , wherein the selected exogenous arabinose fermentation pathway gene encodes a polypeptide comprising an amino acid sequence with at least 80% sequence identity to an amino acid sequence selected from the group consisting of the amino acid sequences set forth in SEQ ID Nos: 6 , 8 , 10 , 12 , 14 , 16 , 18 , and 20.18. (canceled)19. The genetically modified yeast cell of further comprising an active xylose fermentation pathway claim 17 , wherein said cell comprises one or more exogenous xylose fermentation pathway genes selected from the group consisting of XR claim 17 , XDH claim 17 , and XK genes.20. The genetically modified yeast cell of further comprising an active xylose fermentation pathway claim 17 , wherein said cell comprises one or more exogenous xylose fermentation pathway genes selected from the group consisting of XI and XK genes.21. The genetically modified yeast cell of further comprising an active non-oxidative pentose phosphate pathway claim 17 , wherein said cell comprises one or more exogenous non-oxidative pentose phosphate pathway genes selected from the group consisting of TKL and TAL genes.2224-. (canceled)25. The genetically modified yeast cell of claim 17 , wherein the AI ...

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Номер записи: 70
05-01-2017 дата публикации

MICROORGANISMS HAVING PUTRESCINE PRODUCTIVITY AND PROCESS FOR PRODUCING PUTRESCINE USING THE SAME

Номер: US20170002386A1
Автор: JUNG Hee Kyoung, KIM Chang Gyeom, LEE Baek Seok, Lee Kyoung Min, Lee Sun Young, LI Hong Xian, Park Su Jin, Um Hye Won, YANG Young Lyeol, YOON Jong Hyun
Принадлежит: CJ CHEILJEDANG CORPORATION

The present invention relates to a recombinant microorganism capable of producing putrescine, in which the microorganism is modified to have enhanced NCgl2522 activity, thereby producing putrescine in a high yield, and a method for producing putrescine using the microorganism. 1. A microorganism having putrescine productivity , which is modified to have enhanced activity of a protein having an amino acid sequence represented by SEQ ID NO: 21 or 23.2. The microorganism having putrescine productivity according to claim 1 , wherein the microorganism is further modified to have weakened activities of ornithine carbamoyltransferase (ArgF) and a protein (NCgl1221) involved in glutamate export claim 1 , compared to the endogenous activities claim 1 , and to have enhanced ornithine decarboxylase (ODC) activity.3. The microorganism having putrescine productivity according to claim 2 , wherein the ornithine carbamoyltransferase (ArgF) has an amino acid sequence represented by SEQ ID NO: 29 claim 2 , the protein (NCgl1221) involved in glutamate export has an amino acid sequence represented by SEQ ID NO: 30 claim 2 , and the ornithine decarboxylase (ODC) has an amino acid sequence represented by SEQ ID NO: 33.4. The microorganism having putrescine productivity according to claim 1 , wherein the microorganism is further modified to have enhanced activities of acetyl-gamma-glutamyl-phosphate reductase (ArgC) claim 1 , acetylglutamate synthase or ornithine acetyltransferase (ArgJ) claim 1 , acetylglutamate kinase (ArgB) claim 1 , and acetylornithine aminotransferase (ArgD) claim 1 , compared to the endogenous activities.5. The microorganism having putrescine productivity according to claim 4 , wherein the acetyl-gamma-glutamyl-phosphate reductase (ArgC) claim 4 , acetylglutamate synthase or ornithine acetyltransferase (ArgJ) claim 4 , acetyl glutamate kinase (ArgB) claim 4 , and acetylornithine aminotransferase (ArgD) have amino acid sequences represented by SEQ ID NOs: 25 claim 4 ...

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Номер записи: 71
04-01-2018 дата публикации

ENZYMES AND METHODS FOR PRODUCING OMEGA-3 FATTY ACIDS

Номер: US20180002633A1
Автор: Blackburn Susan Irene Ellis, Frampton Dion Matthew Frederick, Liu Qing, Mackenzie Anne Maree, Mansour Maged Peter, Nichols Peter David, Petrie James Robertson, Robert Stanley Suresh, Shrestha Pushkar, Singh Surinder Pal, Wood Craig Christopher, Zhou Xue-Rong
Принадлежит:

The present invention relates generally to the field of recombinant fatty acid synthesis, particularly in transgenic plants. The application describes genes involved in fatty acid synthesis and provides methods and vectors for the manipulation of fatty acid composition of plant oils. In particular, the invention provides constructs for achieving the integration of multiple heterologous genes involved in fatty acid synthesis into the plant genome, such that the resulting plants produce altered levels of polyunsaturated fatty acids. Also described are methods for enhancing the expression of fatty acid biosynthesis enzymes by co-expressing a silencing suppressor within the plant storage organ. 1175-. (canceled)176Brassica napus. A cell , comprising exogenous polynucleotides encodinga Δ6 desaturase whose amino acid sequence is set forth as SEQ ID NO:30,a Δ6 elongase,a Δ5 desaturase whose amino acid sequence is provided by Accession No. AF489588,a Δ5 elongase whose amino acid sequence is provided by Accession No. AAV67798, anda Δ4 desaturase whose amino acid sequence is provided by Accession No. AY332747,wherein each exogenous polynucleotide is operably linked to a promoter that directs expression of said polynucleotide in the cell.177Brassica napus. The cell of claim 176 , wherein the Δ6 elongase has the amino acid sequence provided by Accession No. AF428243.178Brassica napusBrassica napus. The cell of which is a seed cell.179Brassica napusBrassica napus. A seed comprising the seed cell of .180Brassica napus. The seed of claim 179 , comprising a total fatty acid content which comprises α-linolenic acid (ALA) claim 179 , stearidonic acid (SDA) claim 179 , eicosatetraenoic acid (ETA) claim 179 , eicosapentaenoic acid (EPA) claim 179 , docosapentaenoic acid (DPA) and docosahexaenoic acid (DHA) claim 179 , wherein the ALA claim 179 , SDA claim 179 , ETA claim 179 , EPA claim 179 , DPA and DHA are each present at a level in the total fatty acid content claim 179 , each level ...

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Номер записи: 72
02-01-2020 дата публикации

MICROORGANISMS FOR PRODUCING PUTRESCINE OR ORNITHINE AND PROCESS FOR PRODUCING PUTRESCINE OR ORNITHINE USING THEM

Номер: US20200002686A1
Автор: JUNG Hee Kyoung, LEE Baek Seok, LEE Hyo Hyoung, Lee Kyoung Min, LI Hong Xian, Park Su Jin, Um Hye Won, YANG Young Lyeol
Принадлежит:

Disclosed is a modified microorganism producing putrescine or ornithine, and a method for producing putrescine or ornithine using the same. 115-. (canceled)16. A method for producing putrescine or ornithine , comprising:{'i': Corynebacterium', 'E. coli', 'E. coli, '(i) culturing a modified microorganism of the genus producing putrescine or ornithine in a medium, wherein activities of N-acetylglutamate synthase from and acetylornithine deacetylase from are introduced into the microorganism; and'}(ii) recovering putrescine or ornithine from the cultured microorganism or the medium.17CorynebacteriumCorynebacterium glutamicum.. The method according to claim 16 , wherein the microorganism of the genus is18E. coliE. coli. The method according to claim 16 , wherein the N-acetylglutamate synthase from consists of an amino acid sequence of SEQ ID NO: 1 claim 16 , and/or the acetylornithine deacetylase from consists of an amino acid sequence of SEQ ID NO: 3.19. The method according to claim 16 , wherein (a) an activity of phosphotransacetylase and acetate kinase operon (pta-ackA operon); (b) an activity of at least one selected from the group consisting of acetyl gamma glutamyl phosphate reductase (ArgC) claim 16 , acetylglutamate synthase or ornithine acetyltransferase (ArgJ) claim 16 , acetylglutamate kinase (ArgB) claim 16 , and acetyl ornithine aminotransferase (ArgD); and/or (c) an activity of putrescine exporter is further enhanced compared to its endogenous activity.20E. coli. The method according to claim 16 , wherein an activity of acetyl-CoA synthetase (acs) from claim 16 , and/or an activity of ornithine decarboxylase (ODC) is further introduced.21. The method according to claim 16 , wherein (a) an activity of i) ornithine carbamoyltransferase (ArgF) claim 16 , ii) glutamate exporter claim 16 , or iii) ornithine carbamoyltransferase and glutamate exporter and/or (b) an activity of acetyltransferase is further weakened compared to its endogenous activity. This ...

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Номер записи: 73
04-01-2018 дата публикации

Synthetic carbon fixation pathways

Номер: US20180002704A1
Автор: Achuthanunni Chokkathukalam, Alex van Eck CONRADIE, Katherine Louise Tibbles, Ramdane Haddouche, Satnam Surae
Принадлежит: Invista North America LLC

The present disclosure relates to methods for more efficiently recycling reduced electron carriers in a hydrogen-oxidizing microorganism with an operable Calvin-Benson cycle; synthetic carbon fixation pathways that recycle reduced electron carriers more efficiently than the Calvin-Benson cycle, such as methods for enzymatically converting carbon dioxide to formate and assimilating the resulting formate into central carbon metabolism; methods for producing biochemical products; and recombinant hosts utilizing one or more synthetic carbon fixation pathways.

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Номер записи: 74
04-01-2018 дата публикации

PRODUCTION OF MEVALONATE, ISOPRENE, AND ISOPRENOIDS USING GENES ENCODING POLYPEPTIDES HAVING THIOLASE, HMG-COA SYNTHASE AND HMG-COA REDUCTASE ENZYMATIC ACTIVITIES

Номер: US20180002727A1
Автор: Beck Zachary Q., Miller Michael C., PERES Caroline M., PRIMAK Yuliya A., PUCCI Jeff P., WELLS Derek H.
Принадлежит:

The invention features compositions and methods for the increased production of mevalonate, isoprene, isoprenoid precursor molecules, and/or isoprenoids in microorganisms via the heterologous expression of the mvaE and mvaS genes from the organisms DSM 20601, EG2, and 2. The cells of claim 1 , wherein the nucleic acids encoding polypeptides of the lower MVA pathway comprise enzymes selected from: (a) an enzyme that phosphorylates mevalonate to mevalonate 5-phosphate; (b) an enzyme that converts mevalonate 5-phosphate to mevalonate 5-pyrophosphate; and (c) an enzyme that converts mevalonate 5-pyrophosphate to isopentenyl pyrophosphate.3M. mazeiM. burtoniiLactobacillusLactobacillus sakeiSaccharomyces cerevisiaeStreptococcusStreptococcus pneumoniaeStreptomycesStreptomyces. The cells of claim 1 , wherein the enzyme that phosphorylates mevalonate to mevalonate 5-phosphate is selected from the group consisting of mevalonate kinase claim 1 , mevalonate kinase polypeptide claim 1 , mevalonate kinase polypeptide claim 1 , mevalonate kinase polypeptide claim 1 , yeast mevalonate kinase polypeptide claim 1 , mevalonate kinase polypeptide claim 1 , mevalonate kinase polypeptide claim 1 , mevalonate kinase polypeptide claim 1 , mevalonate kinase polypeptide claim 1 , and CL190 mevalonate kinase polypeptide.4M. mazei. The cells of claim 3 , wherein the enzyme that phosphorylates mevalonate to mevalonate 5-phosphate is mevalonate kinase.5. The cells of claim 1 , wherein the isoprene synthase polypeptide is a plant isoprene synthase polypeptide or variants thereof.6PuerariaPopulusPopulus alba×Populus tremula. The cells of claim 5 , wherein the isoprene synthase polypeptide is a polypeptide from or or a hybrid claim 5 , claim 5 , or variants thereof.7Pueraria montana, Pueraria lobata, Populus tremuloides, Populus alba, Populus nigraPopulus trichocarpa.. The cells of claim 6 , wherein the isoprene synthase polypeptide is selected from the group consisting of claim 6 , and8Populus ...

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Номер записи: 75
03-01-2019 дата публикации

BIOSYNTHESIS OF POLYKETIDES

Номер: US20190002848A1
Автор: CHEONG Seokjung, CLOMBURG James M., GONZALEZ Ramon
Принадлежит:

This disclosure generally relates to the use of microorganisms to make various functionalized polyketides through polyketoacyl-CoA thiolase-catalyzed non-decarboxylative condensation reactions instead of decarboxylative reactions catalyzed by polyketide synthases. Native or engineered polyketoacyl-CoA thiolases catalyze the non-decarboxylative Claisen condensation in an iterative manner (i.e. multiple rounds) between two either unsubstituted or functionalized ketoacyl-CoAs (and polyketoacyl-CoAs) serving as the primers and acyl-CoAs serving as the extender unit to generate (and elongate) polyketoacyl-CoAs. Before the next round of polyketoacyl-CoA thiolase reaction, the β-keto group of the polyketide chain of polyketoacyl-CoA can be reduced and modified step-wise by 3-OH-polyketoacyl-CoA dehydrogenase or polyketoenoyl-CoA hydratase or polyketoenoyl-CoA reductase. Dehydrogenase converts the β-keto group to β-hydroxy group. Hydratase converts the β-hydroxy group to α-β-double-bond. Reductase converts the α-β-double-bond to single bond. Spontaneous or thioesterase catalyzed termination reaction terminates the elongation of polyketide chain of polyketoacyl-CoA at any point through CoA removal and spontaneous reactions rearrange the structure, generating the final functional polyketide products. 133-. (canceled)34) A method of making a polyketide , comprising growing a genetically engineered microorganism in a nutrient broth for a time sufficient to produce a polyketide and isolating said polyketide or a spontaneously rearranged form of said polyketide or a derivative of said polyketide , wherein said microorganism has a polyketide-producing pathway comprising the following substrate(s) to product(s) conversions:a) C(n)-acyl-CoA+acetyl-CoA→C(n+2)-ketoacyl-CoA;b) C(n+2)-ketoacyl-CoA+acetyl-CoA→C(n+4)-polyketoacyl-CoA;c) optionally, C(n+4)-polyketoacyl-CoA→3-OH—C(n+4)-polyketoacyl-CoA;d) optionally, 3-OH—C(n+4)-polyketoacyl-CoA→C(n+4)-polyketoenoyl-CoA; ande) optionally, C ...

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Номер записи: 76
03-01-2019 дата публикации

METHODS, SYNTHETIC HOSTS AND REAGENTS FOR THE BIOSYNTHESIS OF HYDROCARBONS

Номер: US20190002927A1
Автор: FOSTER Alexander Brett, Pattenden Leonard Keith, Rand Jonathan David, ROBERTS Ana Teresa dos Santos Brito Mendes, Shaw Andrew, Taylor Mark Paul
Принадлежит:

Systems, networks, methods, compositions and recombinant hosts for biosynthesizing hydrocarbons from a feedstock, such as gas, are provided. 1: A method for biosynthesising a hydrocarbon in a recombinant host , said method comprising:providing a fermentation reactor, the fermentation reactor comprising at least one recombinant host, wherein said recombinant host comprises an exogenous nucleic acid sequence encoding a polypeptide having an enzyme activity of EC 2.2.1.7, andproviding a stream comprising a gas or a biological or nonbiological feedstock to the fermentation reactor, andoperating the fermentation reactor at conditions for said recombinant host to metabolize the gas or feedstock and produce the hydrocarbon.3: The method according to claim 1 , wherein said polypeptide having an enzyme activity of EC 2.2.1.7 converts glyceraldehyde-3-phosphate and pyruvate to 1 deoxy-d-xylulose-phosphate.4: The method according to claim 1 , wherein said polypeptide having an enzyme activity of EC 2.2.1.7 is:(i) encoded by a nucleic acid sequence having at least 49% sequence identity to the nucleic acid sequence set forth in SEQ ID NO:4 or 5 or a functional fragment thereof;(ii) encoded by a nucleic acid sequence comprising the nucleic acid sequence set forth in SEQ ID NO:4 or 5 or a functional fragment thereof;(iii) has at least 49% sequence identity to the amino acid sequence set forth in SEQ ID NO:1 or 2 or a functional fragment thereof; or(iv) comprises the amino acid sequence set forth in SEQ ID NO: 1 or 2 or a functional fragment thereof.57-. (canceled)8: The method according to claim 1 , wherein the recombinant host further comprises an exogenous nucleic acid sequence encoding a polypeptide having an enzyme activity of EC 4.2.3.27.10: The method according to claim 8 , wherein said polypeptide having an enzyme activity of EC 4.2.3.27 is:(i) encoded by a nucleic acid sequence having at least 70% sequence identity to the nucleic acid sequence set forth in SEQ ID NO: 6 or ...

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Номер записи: 77
03-01-2019 дата публикации

Recombinant yeast cells producing polylactic acid and uses thereof

Номер: US20190002933A1
Автор: Alain Marty, Athanasios Beopoulos, Florence Bordes, Jean-Marc Nicaud, Jonathan Verbeke, Simon DUSSÉAUX, Sophie Lajus, Vinciane Borsenberger
Принадлежит: Carbios SA, CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS, Institut National de la Recherche Agronomique INRA, Institut National des Sciences Appliquees INSA

The present invention relates to a recombinant yeast cell comprising a gene encoding a protein exhibiting lactyl-CoA synthase activity and a gene encoding a protein exhibiting lactyl-CoA polymerase activity, said recombinant cell having the ability of producing polylactic acid (PLA), and the uses thereof.

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Номер записи: 78
20-01-2022 дата публикации

RECOMBINANT MICROORGANISM CAPABLE OF GROWING USING ONLY CARBON DIOXIDE AND FORMIC ACID AND METHOD FOR PRODUCING USEFUL SUBSTANCES USING THE RECOMBINANT MICROORGANISM

Номер: US20220017879A1
Автор: Ahn Jung Ho, BANG Junho, HWANG Chang Hun, LEE Jong An, LEE Sang Yup
Принадлежит:

Disclosed is a recombinant microorganism capable of growing using only carbon dioxide and formic acid by introducing and improving a metabolic pathway for synthesizing pyruvic acid from carbon dioxide and formic acid to enhance pyruvic acid synthesis efficiency and performing additional genetic manipulation, and a method for producing useful substances using the same. Advantageously, the recombinant microorganism is capable of synthesizing pyruvic acid, a C3 organic compound, at a remarkably improved rate, and in particular, grows well even in a medium containing only carbon dioxide and formic acid as carbon sources without a glucose supply, and is thereby capable of synthesizing pyruvic acid and various high value-added compounds using the same as an intermediate product in an economically efficient manner. 1. A recombinant microorganism , in which a gene encoding a glycine cleavage system transcriptional repressor , pyruvate formate lyase , or phosphoglycerate dehydrogenase is attenuated or deleted from a host microorganism having a formic acid assimilation pathway ,a gene encoding an enzyme involved in a glycine cleavage system reaction is enhancely expressed in the host microorganism having the formic acid assimilation pathway, anda gene encoding formate-tetrahydrofolate ligase, methenyl tetrahydrofolate cyclohydrolase, or methylene-tetrahydrofolate dehydrogenase is introduced into the host microorganism having the formic acid assimilation pathway.2Escherichia, Mannheimia, RhodobacterMethylobacterium. The recombinant microorganism according to claim 1 , wherein the host microorganism is selected from the group consisting of and genera.3. The recombinant microorganism according to claim 1 , wherein the expression of the gene encoding the enzyme involved in the glycine cleavage system reaction is enhanced by substituting a native promoter with a strong promoter.4. The recombinant microorganism according to claim 3 , wherein the strong promoter is selected from the ...

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Номер записи: 79
08-01-2015 дата публикации

Capsular gram-positive bacteria bioconjugate vaccines

Номер: US20150010592A1
Автор: Michael Kowarik, Michael Wacker, Michael Wetter
Принадлежит: GLYCOVAXYN AG

The present invention encompasses a novel S. aureus bioconjugate vaccine. More generally, the invention is directed to Gram-positive and other bioconjugate vaccines containing a protein carrier, at least one polysaccharide such as a capsular Gram-positive polysaccharide, and, optionally, an adjuvant or pharmaceutically acceptable carrier. The instant invention also includes methods of producing Gram-positive and other bioconjugate vaccines. An N-glycosylated protein is also provided that contains one or more polysaccharides such as Gram-positive polysaccharides. The invention is additionally directed to engineered prokaryotic organisms comprising nucleotide sequences encoding a glycosyltransferase of a first prokaryotic organism and a glycosyltransferase of a second prokaryotic organism. The invention further includes plasmids and prokaryotic cells transformed with plasmids encoding polysaccharides and enzymes which produce an N-glycosylated protein and/or bioconjugate vaccine. Further, the invention is directed to methods of inducing an immune response in a mammal comprising administering said bioconjugate vaccines.

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Номер записи: 80
12-01-2017 дата публикации

VECTORS AND STRAINS FOR PRODUCING MYRCENE AND METHOD OF PRODUCING MYRCENE USING THE SAME

Номер: US20170009240A1
Автор: GONG Gyeongtaek, KIM Eun Mi, KIM Yunje, Lee Sun Mi, UM Youngsoon, WOO Han Min
Принадлежит: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Disclosed herein are an expression vector capable of expressing myrcene, an strain transformed with the vector and having improved capability of producing myrcene and a method for producing myrcene and a method for recycling glycerol using the same. In an aspect, the transformed strain of the present disclosure can produce myrcene with high purity on a large scale using glycerol or glucose as a carbon source. Also, the strain of the present disclosure is economical and environment-friendly because it can produce high value-added myrcene using waste glycerol as a carbon source. In addition, the strongly volatile myrcene can be produced and isolated at the same time. 1Escherichia coli. A transformed strain transformed with a first vector and a second vector ,the first vector comprising, in sequence,a chloramphenicol resistance gene as a selection marker;a p15A replication origin as a replication origin;a lacUV5 promoter;a first domain comprising a gene encoding an enzyme which produces mevalonate from acetyl-CoA; anda second domain comprising a gene encoding an enzyme which produces dimethylallyl pyrophosphate (DMAPP) from mevalonate, andthe second vector comprising, in sequence,an ampicillin resistance gene as a selection marker;a ColE1 replication origin as a replication origin;a trc promoter; anda gene encoding an enzyme which is capable of producing myrcene from geranyl pyrophosphate (GPP).2Escherichia coli. The transformed strain according to claim 1 ,wherein the first vector further comprises one or more selected from a trc promoter; and a gene encoding an enzyme which is capable of producing geranyl pyrophosphate (GPP) from dimethylallyl pyrophosphate (DMAPP) and isopentenyl diphosphate (IPP),the trc promoter is located between the first domain and the second domain, andthe gene encoding an enzyme which is capable of producing geranyl pyrophosphate (GPP) from dimethylallyl pyrophosphate (DMAPP) and isopentenyl diphosphate (IPP) is located downstream of the ...

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Номер записи: 81
14-01-2016 дата публикации

Novel Ketoacyl ACP Synthase Genes and Uses Thereof

Номер: US20160010066A1
Автор: Casolari Jason, Davis David, Ewing Aren, Franklin Scott, Rudenko George, Somanchi Aravind
Принадлежит:

The present invention relates to beta-ketoacyl ACP synthase genes of the KASI/KASIV type and proteins encoded by these genes. The genes can be included in nucleic acid constructs, vectors or host cells. Expression of the gene products can alter the fatty acid profile of host cells. The KAS genes can be combined with a FATA or FATB thioesterase gene to create a cell that produces an increased amount of C8-C16 fatty acids. Suitable host cells include plastidic cells of plants or microalgae. Oleaginous microalga host cells with the new genes are disclosed. 1. A non-natural , isolated polynucleotide having at least 80 , 85 , 90 , 91 , 92 , 93 , 94 , 95 , 96 , 97 , 98 , or 99% sequence identity or equivalent sequence by virtue of the degeneracy of the genetic code to any one of SEQ ID NOs: 21-37 , or 39-55 , or encoding a KASI-like protein having at least 80 , 85 , 85.5 , 86 , 86.5 , 87 , 87.5 , 88 , 88.5 , 89 , 89.5 , 90 , 90.5 , 91 , 91.5 , 92 , 92.5 , 93 , 93.5 , 94 , 94.5 , 95 , 95.5 , 96 , 96.5 , 97 , 97.5 , 98 , 98.5 , 99 or 99.5% amino acid sequence identity to any one of SEQ ID NOs: 2-18 , 62-72 , or a mature protein produced therefrom , or the complement of the polynucleotide.2. A transformation vector comprising the cDNA of .3. The vector of claim 2 , comprising promoter and 3′UTR sequences in operable linkage to the cDNA claim 2 , and optionally a flanking sequence for homologous recombination.4. A host cell comprising the vector of .5. The host cell of claim 4 , wherein the host cell is a plastidic oleaginous cell having a type II fatty acid biosynthesis pathway.6. The host cell of claim 5 , wherein the host cell is a microalga.7ChlorellaPrototheca.. The host cell of claim 6 , wherein the host cell is of Trebouxiophyceae claim 6 , and optionally of the genus or8Prototheca moriformis.. The host cell of claim 7 , wherein the microalga is of the species9. A method for making a cell-oil claim 4 , the method comprising cultivating a host cell of claim 4 , so as ...

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Номер записи: 82
14-01-2016 дата публикации

Modified native beta-ketoacyl-acp synthases and engineered microorganisms

Номер: US20160010115A1
Автор: Catherine M. Cho, Fernando Valle, Jonathan VROOM, Louis Clark, Robert Osborne, Smita Shankar
Принадлежит: Codexis Inc

Genetically engineered cells and microorganisms are provided that produce fatty alcohols and fatty acids. In particular, engineered microbial cells comprise a modified native gene having β-ketoacyl-acp synthase activity.

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Номер записи: 83
08-01-2015 дата публикации

NOVEL LYSOPHOSPHOLIPID ACYLTRANSFERASE

Номер: US20150011786A1
Автор: Ochiai Misa
Принадлежит:

The present invention provides novel lysophospholipid acyltransferases. The object of the present invention is attained by the nucleotide sequences of SEQ ID NOs: 1 and 6 and the amino acid sequences of SEQ ID NOs: 2 and 7 of the present invention. 1. A cDNA or recombinant vector comprising a nucleic acid of any one of (a)-(e) below:(a) a nucleic acid that comprises a nucleotide sequence encoding a protein consisting of an amino acid sequence with deletion, substitution or addition of one to 50 amino acids in the amino acid sequence shown in SEQ ID NO: 2, and having lysophospholipid acyltransferase activity;(b) a nucleic acid that hybridizes under hybridization conditions of 0.1×SSC-1×SSC at 60° C.-65° C. and washing conditions of 0.2×SSC-2×SSC at 50° C.-68° C. to a nucleic acid consisting of a nucleotide sequence full length complement to the nucleotide sequence consisting of SEQ ID NO: 1 and that comprises a nucleotide sequence encoding a protein having lysophospholipid acyltransferase activity;(c) a nucleic acid that comprises a nucleotide sequence sharing an identity of 90% or more with the nucleotide sequence consisting of SEQ ID NO: 1 and encoding a protein having lysophospholipid acyltransferase activity;(d) a nucleic acid that comprises a nucleotide sequence encoding a protein consisting of an amino acid sequence sharing an identity of 90% or more with the amino acid sequence consisting of SEQ ID NO: 2 and having lysophospholipid acyltransferase activity; and(e) a nucleic acid that hybridizes under hybridization conditions of 0.1×SSC-1×SSC at 60° C.-65° C. and washing conditions of 0.2×SSC-2×SSC at 50° C.-68° C. to a nucleic acid consisting of a nucleotide sequence full length complement to a nucleotide sequence encoding a protein consisting of the amino acid sequence shown in SEQ ID NO: 2 and that comprises a nucleotide sequence encoding a protein having lysophospholipid acyltransferase activity.2. The cDNA or recombinant vector of claim 1 , which is (a) ...

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Номер записи: 84
11-01-2018 дата публикации

Herbicide-resistant rice plants, polynucleotides encoding herbicide-resistant acetohydroxyacid synthase large subunit proteins, and methods of use

Номер: US20180010101A1
Автор: Alberto Livore, Alberto Raul Prina, Bijay K. Singh, Robert Ascenzi, Sherry R. Whitt
Принадлежит: Instituto Nacional de Tecnologia Agropecuaria INTA

Herbicide-resistant rice plants, isolated polynucleotides that encode herbicide resistant and wild-type acetohydroxy-acid synthase large subunit 1 (AHASL1) polypeptides, and the amino acid sequences of these polypeptides, are described. Expression cassettes and transformation vectors comprising the polynucleotides of the invention, as well as plants and host cells transformed with the polynucleotides, are described. Methods of using the polynucleotides to enhance the resistance of plants to imidazolinone herbicides, and methods for controlling weeds in the vicinity of herbicide-resistant plants are also described.

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Номер записи: 85
11-01-2018 дата публикации

PRODUCTION OF FATTY ACID DERIVATIVES

Номер: US20180010137A1
Автор: Clarke Elizabeth J., Da Costa Bernardo M., Greenfield Derek L., Groban Eli S., HELMAN Noah, Holden Kevin, HU Zhihao, Schirmer Andreas W.
Принадлежит:

The invention relates to compositions and methods, including polynucleotide sequences, amino acid sequences, recombinant host cells and recombinant host cell cultures engineered to produce fatty acid derivative compositions comprising fatty acids, fatty alcohols, fatty aldehydes, fatty esters, alkanes, terminal olefins, internal olefins or ketones. The fatty acid derivative composition is produced extracellularly with a higher titer, yield or productivity than the corresponding wild type or non-engineered host cell. 151.-. (canceled)52. A recombinant host cell comprising a decreased activity of a phosphoenolpyruvate carboxylase (ppc) polypeptide , wherein said recombinant host cell produces a fatty acid derivative composition at a higher titer , yield or productivity than a corresponding wild type host cell when cultured in a medium containing a carbon source under conditions effective to decrease expression of said ppc polypeptide.53. A cell culture comprising the recombinant host cell according to .54. The cell culture of claim 52 , wherein said cell culture comprises a fatty acid derivative composition.55. The cell culture of claim 54 , wherein the fatty acid derivative composition comprises at least one fatty acid derivative selected from the group consisting of fatty acid claim 54 , a fatty ester claim 54 , a fatty alcohol claim 54 , a fatty aldehyde claim 54 , an alkane claim 54 , a terminal olefin claim 54 , an internal olefin claim 54 , and a ketone.56. The cell culture of claim 53 , wherein the fatty acid derivative isa) a C6, C8, C10, C12, C13, C14, C15, C16, C17, or C18 fatty acid derivative, orb) a C10:1, C12:1, C14:1, C16:1, or C18:1 unsaturated fatty acid derivative.57. The cell culture of claim 54 , wherein the fatty acid derivative composition comprises:a) one or more of C8, C10, C12, C14, C16, and C18 fatty acid derivatives,b) fatty acids,c) fatty aldehydes,d) fatty alcohols,e) fatty esters,f) alkanes,g) terminal olefins,h) internal olefins, ori) ...

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Номер записи: 86
11-01-2018 дата публикации

MICROBIAL PRODUCTION OF N-BUTYRALDEHYDE

Номер: US20180010155A1
Автор: Cho Kwang Myung, HIGASHIDE Wendy, Lee Chrissie, RABIZADEH Shahrooz
Принадлежит: Easel Biotechnologies, LLC

Microorganisms and methods of producing n-butyraldehyde with enhanced yields are presented in which a microorganism is engineered to enhance the conversion of a carbon source into n-butyraldehyde. The n-butyraldehyde is recovered by way of a gas stripping process that occurs during the conversion process, providing significantly greater product yield than post-fermentation recovery of n-butyraldehyde alone. 2. The microorganism of claim 1 , wherein the acetyl-CoA acetyltransferase has an E.C. number of 2.3.1.9.3. The microorganism of claim 1 , wherein the 3-hydroxyacyl-CoA dehydrogenase has an E.C. number of 1.1.1.157.4. The microorganism of claim 1 , wherein the crotonyl-CoA hydratase has an E.C. number of 4.2.1.55.5. The microorganism of claim 1 , wherein the butyryl-CoA dehydrogenase has an E.C. number of 1.3.99.2.6. The microorganism of claim 1 , wherein the trans-enoyl-CoA reductase has an E.C. number of 1.3.1.38.7. The microorganism of claim 1 , wherein the butanal dehydrogenase has an E.C. number of 1.2.1.57.8. The microorganism of claim 1 , wherein the at least one native gene is selected from the group consisting of ldhA claim 1 , adhE claim 1 , frdBC claim 1 , pta claim 1 , and yqhD.9. The microorganism of claim 1 , wherein the at least one native gene are ldhA claim 1 , adhE claim 1 , and frdBC.10. The microorganism of claim 1 , wherein the at least one native gene are ldhA claim 1 , adhE claim 1 , frdBC claim 1 , pta claim 1 , and yqhD.11. The microorganism of claim 1 , wherein at least one native gene is deleted to reduce alcohol production by at least 70%.12. The microorganism of claim 1 , wherein at least one native gene is deleted to reduce alcohol production by at least 90%.13. The microorganism of claim 1 , wherein the genetically modified microorganism produces a cumulative yield of 2.0 g/L n-butyraldehyde at or before 40 hours of culture time.14. The microorganism of claim 1 , wherein the genetically modified microorganism produces 50% of a ...

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Номер записи: 87
11-01-2018 дата публикации

ENGINEERED STRAIN OF ESCHERICHIA COLI FOR PRODUCTION OF POLY-R-3-HYDROXYALKANOATE POLYMERS WITH DEFINED MONOMER UNIT COMPOSITION AND METHODS BASED THEREON

Номер: US20180010156A1
Автор: Nomura Christopher T., Tappel Ryan C., Wang Qin
Принадлежит:

Methods and systems for producing prescribed unit size poly(3-hydroxyalkanoate) (PHA) polymers and copolymers are provided. The methods and systems can employ recombinant bacteria that are not native producers of PHA or lack enzymes to degrade PHA once synthesized, metabolize short to long chain fatty acids without induction, and express an (R)-specific enoyl-CoA hydratase and a PHA synthase, the (R)-specific enoyl-CoA hydratase and PHA synthase having wide substrate specificities. The recombinant bacteria are fed at least one fatty acid substrate that is equal in carbon length to the prescribed or desired unit size of the PHA polymer to be produced. The prescribed unit size PHA that is produced is then isolated and/or purified. 1. A recombinant bacterium for producing a prescribed unit size of PHA polymer , wherein the bacterium:metabolizes a short to long chain fatty acid substrate without induction,expresses an (R)-specific enoyl-CoA hydratase and a PHA synthase, andproduces exclusively a prescribed unit size poly(3-hydroxyalkanoate) (PHA) polymer from at least one fatty acid substrate, the at least one fatty acid substrate being of equal carbon length to the prescribed unit size of the PHA polymer to be produced.2. The recombinant bacterium of claim 1 , wherein the bacterium is not a native or natural producer of PHA or lacks enzymes to degrade PHA once synthesized.3E. coli.. The recombinant bacterium of claim 1 , wherein the recombinant bacterium is a recombinant4. The recombinant bacterium of claim 1 , wherein the bacterium comprises a plasmid comprising an (R)-specific enoyl-CoA hydratase gene and a PHA synthase gene.5. The recombinant bacterium of claim 1 , wherein β-oxidation is blocked in the recombinant bacterium.6. The recombinant bacterium of claim 1 , wherein at least one gene encoding an enzyme for β-oxidation is inactive or deleted from the chromosome of the recombinant bacterium.7. The recombinant bacterium of claim 1 , wherein the recombinant ...

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Номер записи: 88
14-01-2021 дата публикации

D-Lactate Dehydrogenase, Engineered Strain Containing D-Lactate Dehydrogenase and Construction Method and Use of Engineered Strain

Номер: US20210010040A1
Автор: Chao Li, Fei Tao, Hongzhi Tang, Ping Xu
Принадлежит: Shanghai Jiaotong University

The present invention provides D-lactate dehydrogenase, an engineered strain containing the D-lactate dehydrogenase and a construction method and use of the engineered strain. The present invention discloses a D-lactate dehydrogenase which has unique properties and is from Thermodesulfatator indicus, and the D-lactate dehydrogenase has good thermophily and heat stability. By using the D-lactate dehydrogenase and said gene engineering reconstruction method, a fermentation product of the reconstructed Bacillus licheniformis can be redirected to optically-pure D-lactic acid with a high yield from naturally produced 2,3-butanediol, and the optical purity of the produced D-lactic acid reaches 99.9%; and raw materials for fermentation are low-cost, and a fermentation state is between an anaerobic fermentation state and a microaerobic fermentation state. By using the inventive method for producing D-lactic acid through fermentation at high temperature, the production cost can be reduced, the production efficiency can be improved and there is a wide industrial application prospect for the inventive method.

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Номер записи: 89
10-01-2019 дата публикации

PRODUCTION OF DHA AND OTHER LC PUFAS IN PLANTS

Номер: US20190010510A1
Автор: Bevan Scott, Gachotte Daniel, Kuner Jerry, Merlo Ann Owens, Metz James, Roessler Paul G., WALSH Terence A
Принадлежит:

The invention provides recombinant host organisms (e.g., plants) genetically modified with a polyunsaturated fatty acid (PUFA) synthase system and one or more accessory proteins (e.g., PPTase and/or ACoAS) that allow for and/or improve the production of PUFAs in the host organism. The present invention also relates to methods of making and using such organisms (e.g., to obtain PUFAs) as well as products obtained from such organisms (e.g., oil and/or seed). 168-. (canceled)69Brassica.. A seed oil obtained from a genetically modified plant , descendant , seed , cell , tissue , or part thereof , wherein said seed oil comprises 0.01% to 15% DHA and wherein said plant is70. The seed oil of claim 69 , wherein said seed oil comprises 0.01% to 10% DHA.71. The seed oil of claim 69 , wherein said seed oil comprises 0.05% to 1% DHA.72. The seed oil of any one of - claim 69 , wherein said seed oil further comprises 0.01% to 10% EPA.73. The seed oil of any one of - claim 69 , wherein said seed oil further comprises 0.01% to 5% EPA.74. The seed oil of any one of - claim 69 , wherein said seed oil further comprises 0.05% to 1% EPA.75. A canola seed oil claim 69 , wherein said seed oil is substantially free of intermediate or side products of the system for synthesizing PUFAs and that are not naturally produced by the endogenous FAS system in the wild-type plants.76. The seed oil of claim 75 , wherein said seed oil comprises less than 7% by weight of total fatty acids of intermediate or side products of the system for synthesizing PUFAs.77. The seed oil of claim 75 , wherein said seed oil comprises less than 5% by weight of total fatty acids of intermediate or side products of the system for synthesizing PUFAs.78. The seed oil of claim 75 , wherein said seed oil comprises less than 3% by weight of total fatty acids of intermediate or side products of the system for synthesizing PUFAs.79. The seed oil of any one of - claim 75 , wherein said intermediate or side products of the ...

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Номер записи: 90
10-01-2019 дата публикации

METHODS OF MAKING CAPSINOIDS BY BIOSYNTHETIC PROCESSES

Номер: US20190010522A1
Автор: Chen Hui, Yu Xiaodan
Принадлежит: Conagen Inc.

Provided herein are methods of making capsinoids including providing a capsiate synthase in a mixture or cellular system, feeding 8-methyl-6-nonenoyl-CoA, 6E-8-methylnonenoic acid or 8-methylnonanoic acid into the mixture or cellular system, feeding vanillyl alcohol into the mixture or cellular system, and collecting capsinoids from the mixture or cellular system. 1. A method of producing a capsinoid , the method comprising:(a) expressing a capsiate synthase (CS) in a cellular system;(b) adding 8-methyl-6-nonenoyl-CoA and vanillyl alcohol to the cellular system; and(c) incubating the cellular system for a sufficient time to produce the capsinoid.2. A method of producing dihydrocapsiate , the method comprising:(a) expressing a capsiate synthase (CS) and an acyltransferase (ACS) in a cellular system;(b) adding 8-methylnonanoic acid and vanillyl alcohol to the cellular system; and(c) incubating the cellular system for a sufficient time to produce the dihydrocapsiate.3. A method of producing capsiate , the method comprising:(a) expressing a capsiate synthase (CS) and an acyltransferase (ACS) in a cellular system;(b) adding 6E-8-methylnonanoic acid and vanillyl alcohol to the cellular system; and(c) incubating the cellular system for a sufficient time to produce the capsiate.4. A method of producing a capsinoid , the method comprising:(a) expressing a capsiate synthase (CS) and an acyltransferase (ACS) in a cellular system;(b) adding a medium chain fatty acid and vanillyl alcohol to the cellular system; and(c) incubating the cellular system for a sufficient time to produce the capsinoid.5Capsicum. The method of any one of to , wherein the CS amino acid sequence is derived from a plant of the genus.6Capsicum. The method of claim 5 , wherein the genus plant is a ghost chili plant.7. The method of any one of to claim 5 , wherein the CS comprises an amino acid sequence having at least 90% identity to SEQ ID NO: 1.8. The method of claim 7 , wherein the CS comprises the amino ...

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Номер записи: 91
10-01-2019 дата публикации

Method of Producing Lipid

Номер: US20190010525A1
Автор: Shinji Sugihara, Tatsuro Ozaki
Принадлежит: Kao Corp

A method of producing lipids, containing the steps of: culturing a transformant in which the expression of a gene encoding the following protein (A) or (B) is enhanced, and producing long-chain fatty acids or the lipids containing these fatty acids as components, wherein: protein (A) is a protein consisting of the amino acid sequence set forth in SEQ ID NO: 1; and protein (B) is a protein consisting of an amino acid sequence having 70% or more identity with the amino acid sequence of the protein (A), and having β-ketoacyl-ACP synthase activity.

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Номер записи: 92
09-01-2020 дата публикации

Genetically Altered Alfalfa Producing Clovamide and/or Related Hydroxycinnamoyl Amides

Номер: US20200010843A1
Автор: Sullivan Michael L.
Принадлежит:

Two novel cDNAs for two different genes, HDT1 and HDT2, are isolated from red clover and sequenced. Both HDT1 and HDT2 encode hydroxycinnamoyl-CoA:L-DOPA/tyrosine hydroxycinnamoyl transferase (HDT) which enzymatically produces clovamide and/or related hydroxycinnamoyl amides. Clovamide and related hydroxycinnamoyl amides reduce post-harvest protein degradation. Genetically altered alfalfa plants containing an expression cassette containing a cDNA encoding HDT1 or HDT2 are generated. These genetically altered alfalfa plants produce hydroxycinnamoyl-CoA:L-DOPA/tyrosine hydroxycinnamoyl transferase, which in turn produces clovamide and/or related hydroxycinnamoyl amides. 126-. (canceled)27. A cDNA comprising a nucleotide sequence selected from the group consisting of SEQ ID NO: 1 , SEQ ID NO: 3 , a sequence that is at least 95% identical to SEQ ID NO: 1 , and a sequence that is at least 95% identical to SEQ ID NO: 3 , wherein said cDNA encodes a protein with hydroxycinnamoyl-CoA:L-DOPA/tyrosine hydroxycinnamoyl transferase activity.28. An expression cassette comprising a heterologous promoter operably linked to said cDNA of .29. A cDNA that encodes a hydroxycinnamoyl-CoA:L-DOPA/tyrosine hydroxycinnamoyl transferase comprising a nucleotide sequence that encodes a hydroxycinnamoyl-CoA:L-DOPA/tyrosine hydroxycinnamoyl transferase wherein said hydroxycinnamoyl-CoA:L-DOPA/tyrosine hydroxycinnamoyl transferase has an amino acid sequence selected from the group consisting of SEQ ID NO: 2 claim 27 , SEQ ID NO: 4 claim 27 , a sequence that is at least 95% identical to SEQ ID NO: 2 claim 27 , and a sequence that is at least 95% identical to SEQ ID NO: 4.30. An expression cassette comprising a heterologous promoter operably linked to said cDNA of .31. A kit for determining if an alfalfa plant contains HDT1 or HDT2 gene and thereby produces a hydroxycinnamoyl-CoA:L-DOPA/tyrosine hydroxycinnamoyl transferase claim 29 , said kit comprising at least one pair of polynucleotides; an ...

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Номер записи: 93
09-01-2020 дата публикации

METHOD FOR THE PRODUCTION OF ISOAMYL ALCOHOL

Номер: US20200010858A1
Автор: Allard Mathieu, Anissimova Maria
Принадлежит: Global Bioenergies

Described is a method for the production isoamyl alcohol (3-methylbutan-1-ol) comprising the enzymatic conversion of 3-methylbutyryl-CoA (isovaleryl-CoA) into isoamyl alcohol comprising: (a) two enzymatic steps comprising (i) first the enzymatic conversion of 3-methylbutyryl-CoA into 3-methylbutyraldehyde (3-methylbutanal or isovaleraldehyde); and (ii) then enzymatically converting the thus obtained 3-methylbutyraldehyde into said isoamyl alcohol; or (b) a single enzymatic reaction in which 3-methylbutyryl-CoA is directly converted into isoamyl alcohol by making use of an alcohol-forming short chain acyl-CoA dehydrogenase/fatty acyl-CoA reductase or an alcohol-forming fatty acyl-CoA reductase (long-chain acyl-CoA:NADPH reductase) (EC 1.2.1.84). Further, described is the above method wherein the 3-methylbutyryl-CoA can be provided by the enzymatic conversion of 3-methylcrotonyl-CoA into said 3-methylbutyryl-CoA. It is also described that the thus obtained isoamyl alcohol can be further enzymatically converted into 3-methylbutyl acetate (isoamyl acetate) as described herein. Described are also recombinant organisms or microorganisms which are capable of performing the above enzymatic conversions. Furthermore, described are uses of enzymes and enzyme combinations which allow the above enzymatic conversions. 1. A method for the production of isoamyl alcohol (3-methylbutan-1-ol) comprising the enzymatic conversion of 3-methylbutyryl-CoA into isoamyl alcohol comprising: (i) first the enzymatic conversion of 3-methylbutyryl-CoA into 3-methylbutyraldehyde; and', '(ii) then enzymatically converting the thus obtained 3-methylbutyraldehyde into said isoamyl alcohol; or, '(a) two enzymatic steps comprising'}(b) a single enzymatic reaction in which 3-methylbutyryl-CoA is directly converted into isoamyl alcohol by making use of an alcohol-forming short chain acyl-CoA dehydrogenase/fatty acyl-CoA reductase or an alcohol-forming fatty acyl-CoA reductase (long-chain acyl-CoA:NADPH ...

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Номер записи: 94
03-02-2022 дата публикации

Provision of malonyl-coa in coryneform bacteria and method for producing polyphenoles and polyketides with coryneform bacteria

Номер: US20220033786A1
Автор: Jan Marienhagen, Lars Milke, Michael Bott, Nicolai Kallscheuer
Принадлежит: FORSCHUNGSZENTRUM JUELICH GMBH

A coryneform bacteria cell with an increased provision of Malonyl-CoA compared to its archetype, wherein the regulation and/or expression of one or more of genes fasB, gltA, accBC and accD1, and/or the functionality of the enzyme encoded by each gene is modified in a targeted manner. The cell may have one or more targeted modifications, including reduced or eliminated functionality of the fatty acid synthase FasB, mutation or partial or complete deletion of the fatty acid synthase encoding gene fasB, and/or reduced functionality of the promoter operatively linked to the citrate synthase gene gtIA, among other targeted modifications.

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Номер записи: 95
03-02-2022 дата публикации

ENGINEERED BIOSYNTHETIC PATHWAYS FOR PRODUCTION OF 2-OXOADIPATE BY FERMENTATION

Номер: US20220033862A1
Автор: Chowdhury Anupam, Edgar Steven M., Shearer Alexander Glennon, Tracewell Cara Ann, Tymoshenko Stepan, WANG Zhihao
Принадлежит: Zymergen Inc.

The present disclosure describes the engineering of microbial cells for fermentative production of 2-oxoadipate and provides novel engineered microbial cells and cultures, as well as related 2-oxoadipate production methods. 1. An engineered microbial cell that expresses a heterologous homocitrate synthase , wherein the engineered microbial cell produces 2-oxoadipate.2. The engineered microbial cell of claim 1 , wherein the engineered microbial cell also expresses a heterologous homoaconitase.3. The engineered microbial cell of or claim 1 , wherein the engineered microbial cell also expresses a heterologous homoisocitrate dehydrogenase.4. The engineered microbial cell of any one of - claim 1 , wherein the engineered microbial cell expresses one or more additional enzyme(s) selected from an additional heterologous homocitrate synthase claim 1 , an additional heterologous homoaconitase claim 1 , or an additional heterologous homoisocitrate dehydrogenase.5. An engineered microbial cell that expresses a non-native homocitrate synthase claim 1 , wherein the engineered microbial cell produces 2-oxoadipate.6. The engineered microbial cell of claim 5 , wherein the engineered microbial cell also expresses a non-native homoaconitase.7. The engineered microbial cell of or claim 5 , wherein the engineered microbial cell also expresses a non-native homoisocitrate dehydrogenase.8. The engineered microbial cell of any one of - claim 5 , wherein the engineered microbial cell expresses one or more additional enzyme(s) selected from an additional non-native homocitrate synthase claim 5 , an additional non-native homoaconitase claim 5 , or an additional non-native homoisocitrate dehydrogenase.98. The engineered microbial cell of claim 5 , wherein the additional enzyme(s) are from a different organism than the corresponding enzyme in -.10. The engineered microbial cell of any of - claim 5 , wherein the engineered microbial cell comprises increased activity of one or more upstream 2- ...

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Номер записи: 96
03-02-2022 дата публикации

PROGRAMMED MICROORGANISMS TO ATTENUATE A DISEASE

Номер: US20220033867A1
Автор: KUMAR Arumbuliyur Sathish, MURALI Panchapagesa Muthuswamy, Raghavan Shriram
Принадлежит:

The present disclosure discloses a recombinant microbe producing podophyllotoxin, or its derivatives, comprising genes encoding phenyl alanine ammonia-lyase (PAL), cinnamate-4-hydroxylate (C4H), 4-coumaroyl CoA-ligase (4CL), hydroxycinnamoyl-CoA quinate hydroxycinnamoyltransferase (HCT), p-coumaroyl quinate 3′-hydroxylase (C3H), caffeoyl CoA O-methyltransferase (CCoAOMT), bifunctional pineresionl-lariciresinol reductase (DIRPLR), secoisolariciresinol dehydrogenase (SDH), cytochrome P450 oxidoreductase CYP719, O-methyltransferase (OMT), cytochrome P450 oxidoreductase CYP71, and 2-oxoglutarate/Fe(II)-dependent dioxygenase (2-ODD). Also disclosed herein is a method for producing podophyllotoxin or its derivatives. Moreover, a method of treating cancer is also disclosed. 1. A recombinant microbe producing podophyllotoxin , or its derivatives , comprising genes encoding phenyl alanine ammonia-lyase (PAL) , cinnamate-4-hydroxylate (C4H) , 4-coumaroyl CoA-ligase (4CL) , hydroxycinnamoyl-CoA quinate hydroxycinnamoyltransferase (HCT) , p-coumaroyl quinate 3′-hydroxylase (C3H) , caffeoyl CoA O-methyltransferase (CCoAOMT) , bifunctional pineresionl-lariciresinol reductase (DIRPLR) , secoisolariciresinol dehydrogenase (SDH) , cytochrome P450 oxidoreductase CYP719 , O-methyltransferase (OMT) , cytochrome P450 oxidoreductase CYP71 , and 2-oxoglutarate/Fe(II)-dependent dioxygenase (2-ODD).2. The recombinant microbe as claimed in claim 1 , wherein the recombinant microbe further comprises gene encoding cytochrome P450 oxidoreductase CYP82D.3. The recombinant microbe as claimed in claim 2 , wherein the recombinant microbe further comprises gene encoding UDP glucosyl transferase.4. The recombinant microbe as claimed in claim 3 , wherein the recombinant microbe further comprises gene encoding 2-Deoxy-d-ribose-5-phosphate aldolase.5. The recombinant microbe as claimed in any one of the - claim 3 , wherein two or more genes are fused to encode fusion proteins.6. The recombinant microbe ...

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Номер записи: 97
19-01-2017 дата публикации

METHOD FOR THE PRODUCTION OF MULTIPLE-UNSATURATED FATTY ACIDS IN TRANSGENIC ORGANISMS

Номер: US20170016015A1
Автор: Abbadi Amine, Bauer Jörg, Cirpus Petra, Domergue Frederic, Heinz Ernst, Kirsch Jelena, Meyer Astrid, QIU Xiao, SPERLING Petra, Vrinten Patricia, Zank Thorsten
Принадлежит:

The present invention relates to a process for the production of polyunsaturated fatty acids in an organism by introducing, into the organism, nucleic acids which encode polypeptides with Δ5-elongase activity. Advantageously, these nucleic acids can be expressed in the organism together with further nucleic acids which encode polypeptides of the biosynthesis of the fatty acid or lipid metabolism. Especially advantageous are nucleic acids which encode Δ6-desaturases, Δ5-desaturases, Δ4-desaturases and/or Δ6-elongases. These desaturases and elongases are advantageously derived from or . The invention furthermore relates to a process for the production of oils and/or triacylglycerides with an elevated content of long-chain polyunsaturated fatty acids, and oils and/or triacylglycerides thus obtained. The invention also relates to the nucleic acids, and constructs, vectors and transgenic organisms comprising the same, as well as oils, lipids and/or fatty acids produced by the process according to the invention and to their use. 1. A process for the production of a transgenic organism having a content of at least 1% by weight of docosahexaenoic acid and/or eicosapentaenoic acid based on the total lipid content of the transgenic organism , said process comprises:a) introducing, into the organism, at least one nucleic acid encoding a polypeptide with Δ6-desaturase activity;b) introducing, into the organism, at least one nucleic acid encoding a polypeptide with Δ6-elongase activity;c) introducing, into the organism, at least one nucleic acid encoding a polypeptide with Δ5-desaturase activity;d) introducing, into the organism, at least one nucleic acid encoding a polypeptide with Δ5-elongase activity; ande) introducing, into the organism, at least one nucleic acid encoding a polypeptide with Δ4-desaturase activity,wherein the at least one nucleic acid encoding a polypeptide with Δ6-desaturase activity comprises:i) the nucleotide sequence of SEQ ID NO: 17, SEQ ID NO: 19, SEQ ...

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Номер записи: 98
19-01-2017 дата публикации

METHOD FOR PRODUCING 1,3-PROPANEDIOL USING MICROORGANISM VARIANT WITH DELETION OF 2,3-BUTANEDIOL SYNTHETIC GENE

Номер: US20170016032A1
Автор: Heo Sun-Yeon, Jung You Ree, KIM Chul Ho, Lee Sung Mok, Oh Baek Rock, Park Jang Min, Seo Jeong-Woo
Принадлежит:

The present invention relates to a method for producing 1,3-propanediol using a mutant microorganism lacking a 2,3-butanediol synthetic gene, and more particularly to a mutant microorganism wherein a gene encoding lactate dehydrogenase and a gene encoding an enzyme which is involved in 2,3-butanediol synthesis are deleted in a microorganism having the ability to produce 1,3-propanediol from glycerol and wherein a gene encoding pyruvate decarboxylase and a gene encoding aldehyde dehydrogenase are introduced or amplified, and to a method of promoting the production of 1,3-propanediol while inhibiting the production of 2,3-butanediol by using the mutant microorganism. The use of the glycerol-fermenting mutant microorganism according to the present invention can significantly increase the production of 1,3-propanediol while minimizing the production of 2,3-butanediol. 1. A mutant microorganism wherein a gene encoding lactate dehydrogenase and a gene encoding an enzyme which is involved in 2 ,3-butanediol synthesis are deleted in a microorganism having the ability to produce 1 ,3-propanediol from glycerol and wherein a gene encoding pyruvate decarboxylase and a gene encoding aldehyde dehydrogenase are introduced or amplified.2. The mutant microorganism of claim 1 , wherein the enzyme which is involved in 2 claim 1 ,3-butanediol synthesis is an acetolactate synthase.3. The mutant microorganism of claim 1 , wherein the gene encoding pyruvate decarboxylase is pdc derived from a stain claim 1 , which has pyruvate decarboxylase activity.4. The mutant microorganism of claim 1 , wherein the gene encoding aldehyde dehydrogenase is aldB derived from a strain claim 1 , which has pyruvate decarboxylase activity.5. The mutant microorganism of claim 1 , which is Klebsiella pneumonia.6. A method for producing 1 claim 1 ,3-propanediol claim 1 , comprising the steps of:{'claim-ref': {'@idref': 'CLM-00001', 'claim 1'}, '(a) culturing the mutant microorganism of in a glycerol-containing ...

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21-01-2016 дата публикации

NON-CO2 EVOLVING METABOLIC PATHWAY FOR CHEMICAL PRODUCTION

Номер: US20160017339A1
Автор: BOGORAD Igor, Liao James C.
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Provided are microorganisms that catalyze the synthesis of chemicals and biochemicals from a suitable carbon source. Also provided are methods of generating such organisms and methods of synthesizing chemicals and biochemicals using such organisms. 1. A recombinant microorganism comprising a non-COevolving metabolic pathway for the synthesis of acetyl phosphate with improved carbon yield beyond 1:2 molar ratio (fructose 6-phosphate:Acetyl phosphate) from a carbon substrate using a pathway comprising an enzyme having (i) fructose-6-phosphoketolase (Fpk) activity and/or xylulose-5-phosphoketolase (Xpk) activity and (ii) a fructose 1 ,6 bisphosphatase or a sedoheptuloase 1 ,6 bisphosphatase activity.2. The recombinant microorganism of claim 1 , wherein the microorganism can convert a sugar phosphate to acetyl phosphate with improved yield beyond those obtained by pathways that involve pyruvate decarboxylation.3. The recombinant microorganism of claim 2 , wherein the sugar phosphate is selected from the group consisting of: sugar phosphates of a triose claim 2 , an erythrose claim 2 , a pentose claim 2 , a hexose claim 2 , and a sedoheptulose.4. The recombinant microorganism of claim 3 , wherein the sugar phosphate of a triose is selected from the group consisting of G3P and DHAP; wherein the pentose is selected from the group consisting of RSP claim 3 , Ru5P claim 3 , RuBP claim 3 , and X5P; wherein the hexose is selected from the group consisting of F6P claim 3 , H6P claim 3 , FBP claim 3 , and G6P; and wherein the sedoheptulose is selected from the group consisting of S7P and SBP.5. The recombinant microorganism of claim 3 , wherein the sugar phosphates are derived from a carbon source selected from the group consisting of methanol claim 3 , methane claim 3 , CO claim 3 , CO claim 3 , formaldehyde claim 3 , formate claim 3 , glycerol claim 3 , a carbohydrate having the general formula CHO claim 3 , wherein n=3 to 7 claim 3 , and cellulose.6. The recombinant ...

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Номер записи: 100