MUTANT HPGG MOTIF AND HDASH MOTIF DELTA-5 DESATURASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS

Mutant delta-5 desaturases, having the ability to convert dihomo-gamma-linolenic acid [DGLA; 20:3 omega-6] to arachidonic acid [ARA; 20:4 omega-6] and/or eicosatetraenoic acid [ETA; 20:4 omega-3] to eicosapentaenoic acid [EPA; 20:5 omega-3] and possessing at least one mutation within the HPGG (SEQ ID NO:7) motif of the cytochome b-like domain and at least one mutation within the HDASH (SEQ ID NO:8) motif are disclosed. Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding delta-5 desaturases, along with a method of making long chain polyunsaturated fatty acids [“PUFAs”], are also disclosed. 1. A mutant polypeptide having delta-5 desaturase activity comprising:(a) an amino acid motif as set forth in SEQ ID NO:34 [HxGx], wherein SEQ ID NO:34 [HxGx] is not identical to SEQ ID NO:7 [HPGG]; and,(b) an amino acid motif as set forth in SEQ ID NO:1 [HxxxH], wherein SEQ ID NO:1 [HxxxH] is not identical to SEQ ID NO:8 [HDASH].2. The mutant polypeptide of claim 1 , wherein:(a) said amino acid motif of (a) is selected from the group consisting of: SEQ ID NO:9 [HgGG], SEQ ID NO:10 [HhGG], SEQ ID NO:11 [HPGs], SEQ ID NO:12 [HcGG], SEQ ID NO:13 [HwGG], and SEQ ID NO:14 [HaGG]; and,(b) said amino acid motif of (b) is selected from the group consisting of: SEQ ID NO:15 [HDsSH], SEQ ID NO:16 [HDsSH], SEQ ID NO:17 [HDAaH], SEQ ID NO:18 [HDAgH] and SEQ ID NO:19 [HeASH].3. The mutant polypeptide of claim 2 , wherein said mutant polypeptide has at least 90% sequence identity based on a BLASTP method of alignment when compared to a polypeptide having a sequence selected from the group consisting of: SEQ ID NO:21 claim 2 , SEQ ID NO:25 and SEQ ID NO:29.4. The mutant polypeptide of having the amino acid sequence selected from the group consisting of: SEQ ID NO:139 [EgD5R*-34g157g] claim 3 , SEQ ID NO:141 [EgD5R*-34g158a] claim 3 , SEQ ID NO:143 [EgD5R*-34g158g] claim 3 , SEQ ID NO:145 [EgD5R*-34h158a] claim 3 , SEQ ID NO:147 [EgD5R*-34h158g] claim 3 , ...

Recombinant microbial host cells for high eicosapentaenoic acid production

Engineered strains of the oleaginous yeast Yarrowia lipolytica are disclosed herein that are capable of producing microbial oil comprising greater than 25 weight percent of eicosapentaenoic acid [EPA], an omega-3 polyunsaturated fatty acid, measured as a weight percent of dry cell weight.

Obligately symbiotic thermophile symbiobacterium toebii SC-1 producing thermostable L-tyrosine phenol-lyase and L-tryptophan indole-lyase and a method for screening its relative bacteria

The present invention relates to a novel obligately symbiotic thermophile Symbiobacterium toebii SC-1(Accession NO: KCTC 0685BP), and a thermostable L-tyrosine phenol-lyase and L-tryptophan indole-lyase produced by Symbiobacterium toebii SC-1. The present invention also relates to a pure culture method of Symbiobacterium toebii SC-1, a method of growth measurement for Symbiobacterium toebu SC-1 and its relative symbiotic bacteria showing low-growth yield using nitrate respiration, and a screening method of relative symbiotic strains of Symbiobacterium toebii SC-1 from the environment using a specific antibody to it. Therefore, the thermostable L-tyrosine phenol-lyase and L-tryptophan indole-lyase produced by Symbiobacterium toebii SC-1 in this invention can provide more stable catalysts in enzymatic biotransformation processes which enzymatically produce valuable medico-amino acids 3,4-dehydroxy-L-phenylalanine (L-DOPA) and L-tryptophan.

Constitutive strong promoter and use thereof

Disclosed herein are an aldolase gene promoter and the use thereof, more particularly, disclosed are a promoter of aldolase gene derived from Lactobacillus casei, having a base sequence of SEQ ID NO: 1, an expression vector containing said promoter, and a recombinant microorganism transformed with said expression vector. The recombinant microorganism transformed with the expression vector containing the disclosed promoter can effectively express a target protein on the surface thereof, and thus will be useful as vaccine vehicles and the like.

Down-regulation of a polynucleotide encoding a Sou2 sorbitol utilization protein to modify lipid production in microbial cells

Recombinant microbial cells are disclosed herein that comprise (i) a down-regulation of an endogenous polynucleotide sequence encoding Sou2 sorbitol utilization protein, and (ii) a polyunsaturated fatty acid (PUFA) biosynthetic pathway. The down-regulation of the polynucleotide sequence encoding Sou2 sorbitol utilization protein can increase the lipid content of the microbial cells and/or decrease the total amount of sugar alcohols produced by the microbial cells. Also disclosed are methods of using the recombinant microbial cells to produce oil containing omega-3 polyunsaturated fatty acids such as EPA.

Mutant HPGG motif and HDASH motif delta-5 desaturases and their use in making polyunsaturated fatty acids

Mutant delta-5 desaturases, having the ability to convert dihomo-gamma-linolenic acid [DGLA; 20:3 omega-6] to arachidonic acid [ARA; 20:4 omega-6] and/or eicosatetraenoic acid [ETA; 20:4 omega-3] to eicosapentaenoic acid [EPA; 20:5 omega-3] and possessing at least one mutation within the HPGG (SEQ ID NO:7) motif of the cytochome b5-like domain and at least one mutation within the HDASH (SEQ ID NO:8) motif are disclosed. Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding delta-5 desaturases, along with a method of making long chain polyunsaturated fatty acids ["PUFAs"], are also disclosed.

LIGHT SOURCE DEVICE USING POLARITY OF MATERIAL AND VEHICLE LAMP HAVING THE SAME

A light source device using a polarity of a material may include a transparent frame forming an inner space accommodating a nonpolarity material and a polarity material; a light source portion irradiating light through the nonpolarity material or the polar material accommodated in the transparent frame; and an electromagnet configured to switch respective positions of the nonpolarity material and the polar material by a magnetic force generated as a current is applied from outside of the light source device. 1. A light source device using a polarity of a material , the light source device comprising:a transparent frame forming an inner space accommodating a nonpolarity material and a polarity material;a light source portion irradiating light through the nonpolarity material or the polar material accommodated in the transparent frame; andan electromagnet configured to switch respective positions of the nonpolarity material and the polar material by a magnetic force generated according to the polarity of the material, wherein the magnetic force is generated as a current is applied from outside of the light source device.2. The light source device of claim 1 , wherein the nonpolarity material and the polarity material have different specific gravities or different colors.3. The light source device of claim 1 , wherein the electromagnet is disposed at a position beside claim 1 , above claim 1 , or below the transparent frame.4. The light source device of claim 3 , wherein the electromagnet is one of a plurality of electromagnets claim 3 , and when the plurality of electromagnets are disposed at a plurality of positions relative to the transparent frame and a current is alternately applied to the plurality of electromagnets claim 3 , the nonpolarity material and the polarity material have different colors irrespective of their specific gravities.5. The light source device of claim 1 , wherein the light source portion is disposed inside the transparent frame and is ...

Promoters and gene expression method by using the promoters

To provide a promoter, a recombinant DNA, a gene expression vector, an expression vector, and a transformant, which are capable of expressing a gene without inducing gene expression with an inducer; and a method for producing a protein and a kit therefor, which can be operated easily and performed inexpensively by convenient and inexpensive steps. An isolated DNA having the nucleotide sequence of SEQ ID NO: 1 or 2 of Sequence Listing or a fragment thereof, wherein the isolated DNA exhibits a constitutive promoter activity in Escherichia coli or a bacterium belonging to the genus Bacillus; an isolated DNA having a nucleotide sequence of a nucleic acid capable of hybridizing to the above DNA, wherein the isolated DNA exhibits a constitutive promoter activity in Escherichia coli or a bacterium belonging to the genus Bacillus; a recombinant DNA comprising the above DNA and a foreign gene, wherein the foreign gene is operably located; a gene expression vector, at least comprising the above DNA ...

Apparatus and method for providing integrated device information

An apparatus and a method for providing integrated device information are provided. The apparatus includes a communication unit that transmits and receives data through a first type of wireless communication and a second type of wireless communication; and a controller that discovers a first wireless communication device supporting the first type of wireless communication, receives first wireless communication device information from the discovered first wireless communication device, discovers a second wireless communication device supporting the second type of wireless communication, receives second wireless communication device information from the discovered second wireless communication device, integrates the first wireless communication device information and the second wireless communication device information, and displays the integrated wireless communication device information.

PEPTIDE-MEDIATED DELIVERY OF RNA-GUIDED ENDONUCLEASE INTO CELLS

A composition is disclosed that comprises at least one protein component of an RNA-guided endonuclease (RGEN) and at least one cell-penetrating peptide (CPP), wherein the RGEN protein component and CPP are covalently or non-covalently linked to each other in an RGEN protein-CPP complex. The RGEN protein-CPP complex can traverse (i) a cell membrane, or (ii) a cell wall and cell membrane, of a cell. The RGEN protein component of an RGEN protein-CPP complex in certain embodiments can be associated with a suitable RNA component to provide an RGEN capable of specific DNA targeting. Further disclosed are compositions comprising at least one protein component of a guide polynucleotide/Cas endonuclease complex and at least one CPP, as well as methods of delivering RGEN proteins into microbial cells, as well as methods of targeting DNA with RGENs. 1. A composition comprising at least one protein component of an RNA-guided endonuclease (RGEN) and at least one cell-penetrating peptide (CPP) ,wherein said protein component and CPP are covalently, or non-covalently, linked to each other in an RGEN protein-CPP complex, andwherein said RGEN protein-CPP complex can traverse (i) a cell membrane, or (ii) a cell wall and cell membrane, of a microbial cell.2. The composition of claim 1 , wherein the protein component of the RGEN is associated with at least one RNA component that comprises a sequence complementary to a target site sequence on a chromosome or episome in the microbial cell claim 1 , wherein the RGEN can bind to the target site sequence claim 1 , and optionally cleave one or both DNA strands at the target site sequence.3. The composition of claim 2 , wherein the RNA component comprises a guide RNA (gRNA) comprising a CRISPR RNA (crRNA) operably linked to a trans-activating CRISPR RNA (tracrRNA).4. The composition of claim 2 , wherein the RGEN can cleave one or both DNA strands at the target site sequence.5. The composition of claim 1 , wherein the RGEN comprises a CRISPR- ...

Promoters and gene expression method by using the promoters

To provide a promoter, a recombinant DNA, a gene expression vector, an expression vector, and a transformant, which are capable of expressing a gene without inducing gene expression with an inducer; and a method for producing a protein and a kit therefor, which can be operated easily and performed inexpensively by convenient and inexpensive steps. An isolated DNA having the nucleotide sequence of SEQ ID NO: 1 or 2 of Sequence Listing or a fragment thereof, wherein the isolated DNA exhibits a constitutive promoter activity in Escherichia coli or a bacterium belonging to the genus Bacillus; an isolated DNA having a nucleotide sequence of a nucleic acid capable of hybridizing to the above DNA, wherein the isolated DNA exhibits a constitutive promoter activity in Escherichia coli or a bacterium belonging to the genus Bacillus; a recombinant DNA comprising the above DNA and a foreign gene, wherein the foreign gene is operably located; a gene expression vector, at least comprising the above DNA ...

ROTATION LIGHT SOURCE LAMP SYSTEM FOR REDUCING CHROMATIC ABERRATION AND VEHICLE USING THE SAME

A rotation light source lamp system is combined with a rotation light source device in which a chromatic aberration correction unit obviates focal distance differences occurring between incident paths “a” of lights emitted by LED light sources sequentially synchronized and turned on when first to N-th LED chips arrive at a location where each LED chip faces a signal transmitter while the first to N-th LED chips are rotated once in response to the application of a lamp turn-on signal for the vehicle from the signal transmitter. Chromatic aberration can be reduced by correcting a difference between refractive indices for each wavelength having a different color in the first to N-th LED chips.

High eicosapentaenoic acid oils from improved optimized strains of Yarrowia lipolytica

Described are engineered strains of the oleaginous yeast Yarrowia lipolytica capable of producing an oil comprising greater than 50 weight percent of eicosapentaenoic acid [“EPA”], an ω-3 polyunsaturated fatty acid, measured as a weight percent of total fatty acids [“% TFAs”] and having a ratio of at least 3.1 of EPA % TFAs, to linoleic acid, measured as % TFAs. These strains over-express at least one Δ9 elongase/Δ8 desaturase multizyme, in addition to other heterologous Δ9 elongases, Δ8 desaturases, Δ5 desaturases, Δ17 desaturases, Δ12 desaturases, C 16/18 elongases, and optionally over-express diacylglycerol cholinephosphotransferases, malonyl CoA synthetases and/or acyl-CoA lysophospholipid acyltransferases. The strains possess at least one peroxisome biogenesis factor protein knockout. Methods for producing EPA within said host cells, oils obtained from the cells, and products therefrom are claimed.

Plasmid having a function of t-vector and expression vector, and expression of the target gene using the same

The present invention relates to a plasmid (pHCE-FOREX) functioning as both a T-vector and an expression vector, which is produced by imparting a T-vector function to an HCE promoter derived from a constitutive high-level expression vector and can express a target protein in a simple and rapid manner. Also, the present invention relates to an expression vector having the target gene inserted into the plasmid, and the expression of the target gene using the same. The plasmid of the present invention can be converted into a vector that expresses the target protein by one-step T-vector cloning in a simple and rapid manner. The plasmid converted into the expression vector does not require a re-transformation step and allows the high-level expression of the target protein only by the culturing of transformed E. coli without the addition of an expensive inducer. Thus, according to the present invention, expression plasmids for large amounts of target genes can be produced at the same time, so ...

HIGH EICOSAPENTAENOIC ACID OILS FROM IMPROVED OPTIMIZED STRAINS OF YARROWIA LIPOLYTICA

Described are engineered strains of the oleaginous yeast Yarrowia lipolytica capable of producing an oil comprising greater than 50 weight percent of eicosapentaenoic acid [“EPA”], an ω-3 polyunsaturated fatty acid, measured as a weight percent of total fatty acids [“% TFAs”] and having a ratio of at least 3.1 of EPA % TFAs, to linoleic acid, measured as % TFAs. These strains over-express at least one Δ9 elongase/Δ8 desaturase multizyme, in addition to other heterologous Δ9 elongases, Δ8 desaturases, Δ5 desaturases, Δ17 desaturases, Δ12 desaturases, C16/18 elongases, and optionally over-express diacylglycerol cholinephosphotransferases, malonyl CoA synthetases and/or acyl-CoA lysophospholipid acyltransferases. The strains possess at least one peroxisome biogenesis factor protein knockout. Methods for producing EPA within said host cells, oils obtained from the cells, and products therefrom are claimed.

Obligately symbiotic thermophile Symbiobacterium toebii SC-1 producing thermostable L-tyrosine phenol-lyase and L-tryptophan indole-lyase

The present invention relates to a novel obligately symbiotic thermophile Symbiobacterium toebii SC-1 (Accession NO: KCTC 0685BP), and a thermostable L-tyrosine phenol-lyase and L-tryptophan indole-lyase produced by Symbiobacterium toebii SC-1. The present invention also relates to a pure culture method of Symbiobacterium toebii SC-1, a method of growth measurement for Symbiobacterium toebii SC-1 and its relative symbiotic bacteria showing low-growth yield using nitrate respiration, and a screening method of relative symbiotic strains of Symbiobacterium toebii SC-1 from the environment using a specific antibody to it. Therefore, the thermostable L-tyrosine phenol-lyase and L-tryptophan indole-lyase produced by Symbiobacterium toebii SC-1 in this invention can provide more stable catalysts in enzymatic biotransformation processes which enzymatically produce valuable medico-amino acids 3,4-dehydroxy-L-phenylalanine (L-DOPA) and L-tryptophan.

SURFACE EXPRESSION METHOD OF PEPTIDES P5 AND ANAL3 USING THE GENE ENCODING POLY-GAMMA-GLUTAMATE SYNTHETASE

The present invention relates to a method for expressing each of peptide antibiotics P5 3 and Anal3 35 having amphiphilicity and showing antibacterial, antifungal and anticancer activities 61, 63, 65, 67, 69, 71, on the microbial surface, using a vector containing outer membrane protein genes (pgsBCA) that are derived from Bacillus sp. strains and involved in the synthesis of poly-gamma-glutamate. Moreover, the present invention relates to lactic acid-forming bacteria having each of the peptide antibiotics P5 15 and Anal3 43 expressed on their surface, and the use thereof. According to the present invention, the peptide antibiotics can be expressed on the surface of various microorganisms transformed with the surface expression vectors. The inventive method for the surface expression of the peptide antibiotics allows the peptide antibiotics to be mass-produced without a purification process. Thus, the inventive method has very high industrial applicability. Further, the present invention ...

LAMP DEVICE FOR VEHICLE

A lamp device for a vehicle is provided. The lamp device includes a light source, a lamp housing that accommodates the light source thereon. A lens is coupled to the lamp housing to allow light emitted from the light source to penetrate the lens and a bezel is coupled to the lamp housing and is disposed between the lens and the light source. In particular, the bezel has a surface structure formed on a rear surface thereof and is configured to reflect irradiated light. The bezel is formed in a color that is complementary to a color of the lens. 1. A lamp device for a vehicle , comprising:a light source;a lamp housing that accommodates the light source thereon;a lens coupled to the lamp housing to allow light emitted from the light source to penetrate the lens; anda bezel coupled to the lamp housing, and disposed between the lens and the light source,wherein the bezel includes a surface structure formed on a rear surface thereof and configured to reflect irradiated light.2. The lamp device for the vehicle of claim 1 , wherein the bezel includes a color which is a complementary color to a lens color.3. The lamp device for the vehicle of claim 1 , wherein the rear surface of the bezel is formed to have a rear reflector (RR) shape by which the light emitted from the light source is substantially reflected rearward.4. The lamp device for the vehicle of claim 3 , further comprising:a reflection plate coupled to the lamp housing, and disposed behind the light source.5. The lamp device for the vehicle of claim 1 , wherein the rear surface of the bezel is formed to have a multi focus reflector (MFR) surface configured to reflect the light emitted from the light source at a reflection angle equal to an incident angle.6. The lamp device for the vehicle of claim 5 , further comprising:an additional light source disposed between the bezel and the lamp housing.7. A lamp device for a vehicle claim 5 , comprising:a light source;a lamp housing that accommodates the light source ...

Peroxisome biogenesis factor protein (PEX) disruptions for altering polyunsaturated fatty acids and total lipid content in oleaginous eukaryotic organisms

Methods of increasing the amount of polyunsaturated fatty acids (PUFAs) in the total lipid fraction and in the oil fraction of PUFA-producing, oleaginous eukaryotes, accomplished by modifying the activity of peroxisome biogenesis factor (Pex) proteins, are disclosed. Disruptions of a chromosomal Pex3 gene, Pex10p gene or Pex16p gene in a PUFA-producing, oleaginous eukaryotic strain resulted in an increased amount of PUFAs, as a percent of total fatty acids and as a percent of dry cell weight, in the total lipid fraction and in the oil fraction of the strain, as compared to the parental strain whose native Pex protein was not disrupted.

Increased oil content by increasing Yap1 transcription factor activity in oleaginous yeasts

Transgenic oleaginous yeast having increased oil content comprising increased Yap1 transcription factor activity, wherein the increased oil content is compared to the oil content of a non-transgenic oleaginous yeast, are described herein. The increased Yap1 transcription factor activity results from overexpressing a Yap1 transcription factor, by increasing the interaction between the transcription factor and a protein that is capable of activating the transcription factor, or by a combination thereof. Methods of using these yeast strains are also described.

Cell surface expression vector of sars virus antigen and microorganisms transformed thereby

The present invention relates to a surface expression vector of SARS coronavirus antigen containing a gene encoding an antigen of SARS inducing coronavirus and any one or two or more of genes pgsB, pgsC and pgsA encoding poly-gamma-glutamic acid synthase complex, a microorganism transformed by the surface expression vector, and a SARS vaccine comprising the microorganism. According to the present invention, it is possible to economically produce a vaccine for prevention and treatment of SARS using a recombinant strain expressing an SARS coronavirus antigen on their surface.

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Apparatus for compensating for process variation of resistor in electronic circuit

An electronic circuit apparatus for compensating for a process variation of a resistor in an electronic circuit is provided. The electronic circuit includes a detecting part for generating a tune voltage corresponding to a process variation value of the at least one resistor, and a compensating part for compensating for a process variation of the at least one resistor using the tune voltage.

EXPRESSION OF CALEOSIN IN RECOMBINANT OLEAGINOUS MICROORGANISMS TO INCREASE OIL CONTENT THEREIN

Recombinant oleaginous microorganisms having increased oil content due to the expression of a caleosin polypeptide are described. A recombinant oleaginous microorganism of the disclosed invention produces at least 25% of its dry cell weight as oil, and comprises a functional polyunsaturated fatty acid (PUFA) biosynthetic pathway and at least one genetic construct encoding a caleosin polypeptide. A method for increasing the amount of oil in a recombinant oleaginous microorganism is also described.

Light source device using polarity of material and vehicle lamp having the same

A light source device using a polarity of a material may include a transparent frame forming an inner space accommodating a nonpolarity material and a polarity material; a light source portion irradiating light through the nonpolarity material or the polar material accommodated in the transparent frame; and an electromagnet configured to switch respective positions of the nonpolarity material and the polar material by a magnetic force generated as a current is applied from outside of the light source device.

Multi-faceted reflective hidden lighting lamp and vehicle thereof

A multi-faceted reflective hidden lighting lamp applied to a vehicle may block a portion of external light and repeatedly reflect internal light in two or more steps with a reflective surface on one side surface of a light reflective portion having a triangular sawtooth shape formed on a lens in a direction facing a light source and a transmissive surface on the other side surface thereof in an internal space of the lamp, and form a reflective layer on the reflective surface in painting/deposition/plating processes, a laser perforation process, a masking process, and a film insert process to reduce a color conversion material for the same color conversion effect and increase the utilization of an overlap skin portion of the lens, increasing the light blocking ability for a region other than the region required by regulations in addition to increasing the transmissive/light-receiving ability for the region required by regulations.

ROTATION LIGHT SOURCE DEVICE AND LAMP SYSTEM THEREOF

A lamp system applied to a vehicle according to the present disclosure is provided with any one of a reflector, an optical member, a digital micromirror display (DMD), a shield, or a shield optical module, and combined with a rotation light source device for generating light of a specific LED turned on at a synchronized rotation position of one or more LED chips of first to Nth LED chips (N is an integer of 2 or more) per one rotation while being rotated by a current application of a signal transmitter receiving a lamp turn-on signal of the vehicle, thereby generating various lighting patterns even while eliminating all problems of increasing the layout/decreasing the light amount/increasing the amount of property changed, lowering the reflection efficiency/transmission efficiency, and losing the optical efficiency with the circular LED array.

Hidden lighting lamp using color conversion materials and vehicles having same

A hidden lighting lamp uses a color conversion material and is applied to a vehicle. A color conversion member is spaced apart from a light source by a light source air gap (La) such that the color conversion member is integrally engaged with a lens for projecting light of the light source to the outside or such that the color conversion member is spaced apart from the lens by a lens air gap (Lb). Hidden lighting without deterioration of optical efficiency of LED light from the light source is thereby implemented. One or more of a black painting, a coating layer, an application layer, and a deposition layer are provided, thereby providing concealment of the lamp inner space of the lens, anti-peeling of a deposit, and diversity of fluorescent color.

Down-regulation of a polynucleotide encoding a Sou2 sorbitol utilization protein to modify lipid production in microbial cells

Recombinant microbial cells are disclosed herein that comprise (i) a down-regulation of an endogenous polynucleotide sequence encoding Sou2 sorbitol utilization protein, and (ii) a polyunsaturated fatty acid (PUFA) biosynthetic pathway. The down-regulation of the polynucleotide sequence encoding Sou2 sorbitol utilization protein can increase the lipid content of the microbial cells and/or decrease the total amount of sugar alcohols produced by the microbial cells. Also disclosed are methods of using the recombinant microbial cells to produce oil containing omega-3 polyunsaturated fatty acids such as EPA.

Surface expression method of peptides P5 and ANAL3 using the gene encoding poly-gamma-glutamate synthetase

The present invention relates to a method for expressing each of peptide antibiotics P5 3 and Anal3 35 having amphiphilicity and showing antibacterial, antifungal and anticancer activities 61, 63, 65, 67, 69, 71, on the microbial surface, using a vector containing outer membrane protein genes (pgsBCA) that are derived from Bacillus sp. strains and involved in the synthesis of poly-gamma-glutamate. Moreover, the present invention relates to lactic acid-forming bacteria having each of the peptide antibiotics P5 15 and Anal3 43 expressed on their surface, and the use thereof. According to the present invention, the peptide antibiotics can be expressed on the surface of various microorganisms transformed with the surface expression vectors. The inventive method for the surface expression of the peptide antibiotics allows the peptide antibiotics to be mass-produced without a purification process. Thus, the inventive method has very high industrial applicability. Further, the present invention ...

INCREASED OIL CONTENT BY INCREASING YAP1 TRANSCRIPTION FACTOR ACTIVITY IN OLEAGINOUS YEASTS

Transgenic oleaginous yeast having increased oil content comprising increased Yap1 transcription factor activity, wherein the increased oil content is compared to the oil content of a non-transgenic oleaginous yeast, are described herein. The increased Yap1 transcription factor activity results from overexpressing a Yap1 transcription factor, by increasing the interaction between the transcription factor and a protein that is capable of activating the transcription factor, or by a combination thereof. Methods of using these yeast strains are also described. 1. A transgenic oleaginous yeast having increased oil content comprising increased Yap1 transcription factor activity wherein the increased oil content is compared to the oil content of a non-transgenic oleaginous yeast.2. The transgenic oleaginous yeast of wherein the increased Yap1 transcription factor activity results from overexpressing the Yap1 transcription factor claim 1 , by increasing the interaction between the transcription factor and a protein that is capable of activating the transcription factor claim 1 , or by a combination thereof.3. The transgenic oleaginous yeast of wherein the protein that is capable of activating the transcription factor is selected from the group consisting of: Gpx3 claim 2 , Ybp1 and Tsa1.4. The transgenic oleaginous yeast of claim 2 , wherein the Yap1 transcription factor comprises a nucleotide sequence encoding a polypeptide having transcription factor activity and comprising:a) a bZIP leucine zipper motif;b) an N-terminal Cys-rich domain comprising a sequence of at least two cysteine residues that are separated by at least 6 amino acids; and,c) a C-terminal Cys-rich domain comprising a sequence of at least two cysteine residues that are separated by at least 8 amino acids.5. The transgenic oleaginous yeast of claim 4 , wherein the sequence of the Yap1 transcription factor is selected from the group consisting of SEQ ID NO:2 and SEQ ID NO:4.6. The transgenic oleaginous ...

Garnishless type hidden lamp for vehicle

A garnishless type hidden lamp applied to a vehicle, may include a light source module, in which an internal lens spaced from an external lens, appearing as a garnish from outside thereof due to a color of a penetrable painting portion which reflects the external light, with an air gap interposed therebetween transmits an internal light of a light source through laser perforation holes to form a laser pattern on the external lens, so that the garnishless type hidden lamp may have the sense of unity with vehicle design by a painting garnish function of the external lens without combination with a separate metal garnish, improve a wow effect using the painting garnish, implement differentiated and customized lighting by only changing the laser pattern.

USE OF SACCHAROMYCES CEREVISIAE SUC2 GENE IN YARROWIA LIPOLYTICA FOR SUCROSE UTILIZATION

Disclosed herein are transformed Yarrowia lipolytica comprising an exogenous polynucleotide encoding a polypeptide having sucrose invertase activity. Also disclosed are methods of using the transformed Y. lipolytica.

DOWN-REGULATION OF A POLYNUCLEOTIDE ENCODING A SOU2 SORBITOL UTILIZATION PROTEIN TO MODIFY LIPID PRODUCTION IN MICROBIAL CELLS

Recombinant microbial cells are disclosed herein that comprise (i) a down-regulation of an endogenous polynucleotide sequence encoding Sou2 sorbitol utilization protein, and (ii) a polyunsaturated fatty acid (PUFA) biosynthetic pathway. The down-regulation of the polynucleotide sequence encoding Sou2 sorbitol utilization protein can increase the lipid content of the microbial cells and/or decrease the total amount of sugar alcohols produced by the microbial cells. Also disclosed are methods of using the recombinant microbial cells to produce oil containing omega-3 polyunsaturated fatty acids such as EPA. 1. A recombinant microbial cell comprisinga down-regulation of an endogenous polynucleotide sequence encoding Sou2 sorbitol utilization protein,wherein said down-regulation decreases the total amount of sugar alcohols produced by the microbial cell, as compared to a suitable control cell.2. The recombinant microbial cell of claim 1 , wherein said down-regulation is due to a mutation of said endogenous polynucleotide sequence.3. The recombinant microbial cell of claim 2 , wherein said mutation is selected from the group consisting of a substitution claim 2 , deletion and insertion.4. The recombinant microbial cell of claim 3 , wherein said mutation is a deletion that removes(i) one or more nucleotides from an open reading frame encoding said Sou2 sorbitol utilization protein, and/or(ii) one or more nucleotides of a non-protein-coding sequence located within 500 base pairs of the 5′-end of said open reading frame.5. The recombinant microbial cell of claim 3 , wherein said mutation is an insertion that occurs within(i) an open reading frame encoding said Sou2 sorbitol utilization protein, or(ii) a non-protein-coding sequence located within 500 base pairs of the 5′-end of said open reading frame.67-. (canceled)8. The recombinant microbial cell of claim 1 , wherein said sugar alcohols comprise arabitol or mannitol.9. The recombinant microbial cell of claim 1 , wherein ...

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Peroxisome biogenesis factor protein (Pex) disruptions for altering polyunsaturated fatty acids and total lipid content in oleaginous eukaryotic organisms

Methods of increasing the amount of polyunsaturated fatty acids (PUFAs) in the total lipid fraction and in the oil fraction of PUFA-producing, oleaginous eukaryotes, accomplished by modifying the activity of peroxisome biogenesis factor (Pex) proteins. Disruptions of a chromosomal Pex3 gene, Pex10p gene or Pex16p gene in a PUFA-producing, oleaginous eukaryotic strain resulted in an increased amount of PUFAs, as a percent of total fatty acids and as a percent of dry cell weight, in the total lipid fraction and in the oil fraction of the strain, as compared to the parental strain whose native Pex protein was not disrupted.

Peptide-mediated delivery of RNA-guided endonuclease into cells

A composition is disclosed that comprises at least one protein component of an RNA-guided endonuclease (RGEN) and at least one cell-penetrating peptide (CPP), wherein the RGEN protein component and CPP are covalently or non-covalently linked to each other in an RGEN protein-CPP complex. The RGEN protein-CPP complex can traverse (i) a cell membrane, or (ii) a cell wall and cell membrane, of a cell. The RGEN protein component of an RGEN protein-CPP complex in certain embodiments can be associated with a suitable RNA component to provide an RGEN capable of specific DNA targeting. Further disclosed are compositions comprising at least one protein component of a guide polynucleotide/Cas endonuclease complex and at least one CPP, as well as methods of delivering RGEN proteins into microbial cells, as well as methods of targeting DNA with RGENs.

RECOMBINANT MICROBIAL HOST CELLS FOR HIGH EICOSAPENTAENOIC ACID PRODUCTION

Engineered strains of the oleaginous yeast Yarrowia lipolytica are disclosed herein that are capable of producing microbial oil comprising greater than 25 weight percent of eicosapentaenoic acid [“EPA”], an omega-3 polyunsaturated fatty acid, measured as a weight percent of dry cell weight.

Use of Saccharomyces cerevisiae SUC2 gene in Yarrowia lipolytica for sucrose utilization

Disclosed herein are transformed Yarrowia lipolytica comprising an exogenous polynucleotide encoding a polypeptide having sucrose invertase activity. Also disclosed are methods of using the transformed Y. lipolytica.

ANTICOAGULANT AND COMPOSITION FOR PREVENTING THROMBUS CONTAINING POLY-GAMMA-GLUTAMIC ACID

The present invention relates to an anticoagulant and a composition for preventing thrombus formation, which contain poly-gamma-glutamic acid (PGA) as an active ingredient. The inventive PGA is a water-soluble, anionic, biodegradable and edible amino acid polymer material, which has an anticoagulant effect of preventing thrombi from being accumulated in blood vessels, shows an excellent sustained-release effect and is harmless to the human body. Thus, it is useful as a high-value-added anticoagulant and a food or beverage composition for preventing thrombus formation.

PEROXISOME BIOGENESIS FACTOR PROTEIN (PEX) DISRUPTIONS FOR ALTERING POLYUNSATURATED FATTY ACIDS AND TOTAL LIPID CONTENT IN OLEAGINOUS EUKARYOTIC ORGANISMS

Methods of increasing the amount of polyunsaturated fatty acids (PUFAs) in the total lipid fraction and in the oil fraction of PUFA-producing, oleaginous eukaryotes, accomplished by modifying the activity of peroxisome biogenesis factor (Pex) proteins. Disruptions of a chromosomal Pex3 gene, Pex10p gene or Pex16p gene in a PUFA-producing, oleaginous eukaryotic strain resulted in an increased amount of PUFAs, as a percent of total fatty acids and as a percent of dry cell weight, in the total lipid fraction and in the oil fraction of the strain, as compared to the parental strain whose native Pex protein was not disrupted.

Surface expression vectors having pgsBCA the gene coding poly-gamma-glutamate synthetase, and a method for expression of target protein at the surface of microorganism using the vector

The present invention relates to a surface expression vector having pgsBCA, a gene coding poly-gamma-glutamate synthetase and a method for expression of target protein at the surface of microorganism using the vector. The vector, in which foreign genes are inserted, transforms microorganisms and makes foreign proteins expressed stably on the surface of microorganisms.

METHODS AND COMPOSITIONS FOR POLYMERASE II (POL-II) BASED GUIDE RNA EXPRESSION

Compositions and methods are provided for editing nucleotides and/or altering target sites in the genome of a cell. The methods and compositions employ a recombinant DNA construct comprising a Pol-II promoter operably linked to a polynucleotide encoding a single guide RNA, wherein said guide RNA is capable of forming a guide RNA/Cas endonuclease complex, wherein said complex can bind to and cleave a target site sequence in the genome of a cell such as a eukaryote cell. The present disclosure further describes methods and compositions employing said recombinant DNA construct to modify the genome of non-conventional yeast. 1. A recombinant DNA construct comprising a Polymerase II (Pol-II) promoter operably linked to a polynucleotide encoding a single guide RNA , wherein said guide RNA is capable of forming a guide RNA/Cas endonuclease complex , wherein said complex can bind to and cleave a target site sequence in the genome of a eukaryote.2. A recombinant DNA construct comprising a Polymerase II (Pol-II) promoter operably linked to a polynucleotide encoding a dual guide RNA (crRNA and tracrRNA) , wherein the dual guide RNA is capable of forming a guide RNA/Cas endonuclease complex , wherein said complex can bind to and cleave a target site sequence in the genome of a eukaryote.3. A eukaryote comprising the recombinant DNA of or .4. The recombinant DNA construct of or , wherein the eukaryote is selected from the group comprising a microbe , a yeast , a non-conventional yeast , a fungus , a plant , an archae , a non-human animal , an insect and a nematode.5. A single or dual guide RNA encoded by the recombinant DNA of .6. An expression vector comprising at least one recombinant DNA of .7. The expression vector of claim 6 , further comprising a nucleotide encoding a Cas endonuclease.8. The expression vector of claim 6 , wherein the vector further comprises at least one nucleotide encoding a polynucleotide modification template or donor DNA.9. A method for modifying a ...

Genetic targeting in non-conventional yeast using an RNA-guided endonuclease

Non-conventional yeasts are disclosed herein comprising at least one RNA-guided endonuclease (RGEN) comprising at least one RNA component that does not have a 5′-cap. This uncapped RNA component comprises a sequence complementary to a target site sequence in a chromosome or episome in the yeast. The RGEN can bind to, and optionally cleave, one or both DNA strands at the target site sequence. An example of an RGEN herein is a complex of a Cas9 protein with a guide RNA. A ribozyme is used in certain embodiments to provide an RNA component lacking a 5′-cap. Further disclosed are methods of genetic targeting in non-conventional yeast.

Lamp device for vehicle

A lamp device applied to a vehicle may include a flow change generation device which accelerates an air flow in a space around a heat sink dissipating the heat due to the radiation of the light of a light source of an optical module to form the flow enhancing heat-dissipation efficiency of the heat sink, and generates the flow by the shake due to any one of an inertia force of a vehicle, a magnetic force, and a combination of the inertial force of the vehicle and the magnetic force, enhancing heat-dissipation efficiency by the heat dissipation using a change in the flow around a heat source together with the heat dissipation due to the heat sink.

Surface expression vectors having pgsbca the gene coding poly-gamma-glutamate synthetase, and a method for expression of target protein at the surface of microorganism using the vector

The present invention relates to a surface expression vector having pgsBCA, a gene coding poly-gamma-glutamate synthetase and a method for expression of target protein at the surface of microorganism using the vector. The vector, in which foreign genes are inserted, transforms microorganisms and makes foreign proteins expressed stably on the surface of microorganisms.

Vehicle lamp with rotating light source

A vehicle lamp with a rotating light source may include a signal receiver which receives a signal from one or more sensors provided in a vehicle; a light emitting diode (LED) portion having one or more LED elements configured to emit light toward an outside of the vehicle; a controller connected to the one or more LED elements and configured to control a light generation amount of the one or more LED elements; a signal transmitter which is connected to the signal receiver, receives the signal from the signal receiver and transmits the received signal to the controller; and a driver coupled to the LED portion and configured to rotate the LED portion, wherein the controller controls the light generation amount of the one or more LED elements in a response to the signal.

Novel promoters and gene expression method by using the promoters

To provide a promoter, a recombinant DNA, a gene expression vector, an expression vector, and a transformant, which are capable of expressing a gene without inducing gene expression with an inducer; and a method for producing a protein and a kit therefor, which can be operated easily and performed inexpensively by convenient and inexpensive steps. An isolated DNA having the nucleotide sequence of SEQ ID NO: 1 or 2 of Sequence Listing or a fragment thereof, wherein the isolated DNA exhibits a constitutive promoter activity in Escherichia coli or a bacterium belonging to the genus Bacillus; an isolated DNA having a nucleotide sequence of a nucleic acid capable of hybridizing to the above DNA, wherein the isolated DNA exhibits a constitutive promoter activity in Escherichia coli or a bacterium belonging to the genus Bacillus; a recombinant DNA comprising the above DNA and a foreign gene, wherein the foreign gene is operably located; a gene expression vector, at least comprising the above DNA ...

Method and apparatus for transceiving for beam forming in wireless communication system

An electronic device for beamforming and a method thereof in a wireless communication system are provided. The electronic device includes a plurality of antennas. The electronic device also includes a plurality of transmitter and receiver switches connected to the antennas and configured to select a plurality of transmission paths and a plurality of reception paths. The electronic device further includes a plurality of first Phase Shifters (P/Ss) configured to shift a phase of Radio Frequency (RF) signals received via the antennas and the transmitter and receiver switches. The electronic device includes a combiner configured to combine the phase-shifted RF signals to one RF signal. The electronic device also includes a quadrature signal generator configured to generate a quadrature signal. The electronic device further includes a down-mixer configured to convert the quadrature signal and the combined RF signal to a first baseband signal and configured to output the first baseband signal ...

Rotation light source lamp system for reducing chromatic aberration and vehicle using the same

A rotation light source lamp system is combined with a rotation light source device in which a chromatic aberration correction unit obviates focal distance differences occurring between incident paths “a” of lights emitted by LED light sources sequentially synchronized and turned on when first to N-th LED chips arrive at a location where each LED chip faces a signal transmitter while the first to N-th LED chips are rotated once in response to the application of a lamp turn-on signal for the vehicle from the signal transmitter. Chromatic aberration can be reduced by correcting a difference between refractive indices for each wavelength having a different color in the first to N-th LED chips.

MANIPULATION OF ACYL-COA BINDING PROTEIN EXPRESSION FOR ALTERED LIPID PRODUCTION IN MICROBIAL HOSTS

Acyl-CoA binding protein [“ACBP”] binds thiol esters of long fatty acids and coenzyme A in a one-to-one binding mode with high specificity and affinity. This protein is expected to play an important role in altering lipid production in oleaginous microbial organisms. Knock-out of the protein in the oleaginous yeast, Yarrowia lipolytica, results in a decrease in the total lipid content, while overexpression results in an increase in the total lipid content of the recombinant Yarrowia cells.

High level production of long-chain dicarboxylic acids with microbes

Recombinant microbial cells comprising an engineered LCDA production pathway that comprises at least one up-regulated long-chain acyl-CoA synthetase (ACoS) are disclosed. These recombinant microbial cells are capable of producing one or more long-chain dicarboxylic acid (LCDA) products from a long-chain fatty acid-comprising substrate. Methods of using recombinant microbial cells to produce LCDAs are also disclosed.

RECOMBINANT MICROBIAL CELLS THAT PRODUCE AT LEAST 28% EICOSAPENTAENOIC ACID AS DRY CELL WEIGHT

Recombinant microbial cells are disclosed herein that produce an oil comprising at least 28 percent eicosapentaenoic acid (EPA) measured as a weight percent of dry cell weight. These cells may comprise a polynucleotide sequence encoding an active acyl-CoA:lysophosphatidylcholine acyltransferase (LPCAT) comprising at least one amino acid mutation in a membrane-bound O-acyltransferase motif. In addition, the cells may comprise a down-regulation of an endogenous polynucleotide sequence encoding Sou2 sorbitol utilization protein, and/or one or more polynucleotides encoding phospholipid:diacylglycerol acyltransferase (PDAT), delta-12 desaturase, a dihomo-gamma-linolenic acid (DGLA) synthase multizyme, delta-8 desaturase, malonyl-CoA synthetase (MCS), or acyl-CoA:lysophosphatidic acid acyltransferase (LPAAT). Also disclosed are methods of using the recombinant microbial cells to produce oil containing omega-3 polyunsaturated fatty acids such as EPA. 1. A recombinant microbial cell that produces an oil comprising at least 28 percent eicosapentaenoic acid (EPA) measured as a weight percent of dry cell weight , wherein the cell comprises at least one polynucleotide sequence encoding an acyl-CoA:lysophosphatidylcholine acyltransferase (LPCAT) comprising at least one amino acid mutation in a membrane-bound O-acyltransferase motif , wherein said LPCAT has LPCAT activity , and wherein the polynucleotide encoding LPCAT is operably linked to at least one regulatory sequence.2Yarrowia lipolytica. The recombinant microbial cell of claim 1 , wherein the LPCAT is a LPCAT.3. The recombinant microbial cell of claim 2 , wherein the LPCAT comprises mutations at (i) amino acid position 136 changing methionine to a different amino acid claim 2 , and (ii) amino acid position 389 changing threonine to a different amino acid.4. The recombinant microbial cell of claim 1 , wherein the cell further comprises a down-regulation of an endogenous polynucleotide sequence encoding Sou2 sorbitol ...

Novel promoters and gene expression method by using the promoters

To provide a promoter, a recombinant DNA, a gene expression vector, an expression vector, and a transformant, which are capable of expressing a gene without inducing gene expression with an inducer; and a method for producing a protein and a kit therefor, which can be operated easily and performed inexpensively by convenient and inexpensive steps. An isolated DNA having the nucleotide sequence of SEQ ID NO: 1 or 2 of Sequence Listing or a fragment thereof, wherein the isolated DNA exhibits a constitutive promoter activity in Escherichia coli or a bacterium belonging to the genus Bacillus; an isolated DNA having a nucleotide sequence of a nucleic acid capable of hybridizing to the above DNA, wherein the isolated DNA exhibits a constitutive promoter activity in Escherichia coli or a bacterium belonging to the genus Bacillus; a recombinant DNA comprising the above DNA and a foreign gene, wherein the foreign gene is operably located; a gene expression vector, at least comprising the above DNA ...

LIGHT GUIDE PLATE STAMP AND METHOD OF MANUFACTURING THE SAME

A stamp includes a metal supporting layer, a pattern forming layer and an adhesive layer. The metal supporting layer has a first thermal conductivity. The pattern forming layer is disposed on the metal supporting layer and has a surface with a molding pattern formed thereon. The adhesive layer is disposed between the metal supporting layer and the pattern forming layer to couple the pattern forming layer to the metal supporting layer, and has a second thermal conductivity lower than the first thermal conductivity. Thus, strength of the stamp may be improved, and deformation of the stamp during the process of manufacturing a light guide plate may be reduced or prevented.

Peptide-mediated delivery of RNA-guided endonuclease into cells

A composition is disclosed that comprises at least one protein component of an RNA-guided endonuclease (RGEN) and at least one cell-penetrating peptide (CPP), wherein the RGEN protein component and CPP are covalently or non-covalently linked to each other in an RGEN protein-CPP complex. The RGEN protein-CPP complex can traverse (i) a cell membrane, or (ii) a cell wall and cell membrane, of a cell. The RGEN protein component of an RGEN protein-CPP complex in certain embodiments can be associated with a suitable RNA component to provide an RGEN capable of specific DNA targeting. Further disclosed are compositions comprising at least one protein component of a guide polynucleotide/Cas endonuclease complex and at least one CPP, as well as methods of delivering RGEN proteins into microbial cells, as well as methods of targeting DNA with RGENs.

Method and device for extending beam area in wireless communication system

The present disclosure relates to a pre-5th-generation (5G) or 5G communication system to be provided for supporting higher data rates beyond 4th-generation (4G) communication system such as long term evolution (LTE). An electronic device is provided in a wireless communication system. The device comprises a plurality of antenna sets; a plurality of antenna elements configuring the plurality of antenna sets; an RF transceiver including a plurality of switches for selecting the plurality of antenna elements and a plurality of phase shifters for shifting the phase of a signal transmitted/received through the plurality of antenna elements; and a control unit for determining a beam forming direction and the phase of the signal by simultaneously controlling the plurality of switches and the plurality of phase shifters according to a beambook.

Lamp device for vehicle

A lamp device for a vehicle is provided. The lamp device includes a light source, a lamp housing that accommodates the light source thereon. A lens is coupled to the lamp housing to allow light emitted from the light source to penetrate the lens and a bezel is coupled to the lamp housing and is disposed between the lens and the light source. In particular, the bezel has a surface structure formed on a rear surface thereof and is configured to reflect irradiated light. The bezel is formed in a color that is complementary to a color of the lens.

APPARATUS AND METHOD FOR A VEHICLE RECOGNIZING AN OBJECT USING PATTERN RECOGNITION

An apparatus for recognizing an object of a vehicle using pattern recognition includes a first lamp including: a first optical device configured to generate light of a first pattern and irradiate the light of the first pattern to an object according to a predetermined cycle, and a second optical device configured to generate light of a second pattern and irradiate the light of the second pattern to the object. The apparatus further includes: a camera configured to recognize the first pattern irradiated to the object when the light of the first pattern is irradiated, and a control unit configured to acquire object recognition information of the object based on the first pattern recognized by the camera.

HIGH LEVEL PRODUCTION OF LONG-CHAIN DICARBOXYLIC ACIDS WITH MICROBES

Recombinant microbial cells comprising an engineered LCDA production pathway that comprises at least one up-regulated long-chain acyl-CoA synthetase (ACoS) are disclosed. These recombinant microbial cells are capable of producing one or more long-chain dicarboxylic acid (LCDA) products from a long-chain fatty acid-comprising substrate. Methods of using recombinant microbial cells to produce LCDAs are also disclosed. 1. A recombinant microbial cell comprising an engineered LCDA production pathway that comprises up-regulation of a polynucleotide sequence encoding a long-chain acyl-CoA synthetase (ACoS enzyme) ,wherein said microbial cell can produce one or more long-chain dicarboxylic acid (LCDA) products from a long-chain fatty acid-comprising substrate.2. The recombinant microbial cell of claim 1 , wherein the ACoS enzyme comprises an amino acid sequence that is at least 90% identical to SEQ ID NO:44 claim 1 , 49 claim 1 , 36 claim 1 , 33 claim 1 , or 34.3. The recombinant microbial cell of claim 1 , wherein the ACoS enzyme has both long-chain acyl-CoA synthetase activity and coumaroyl-CoA synthetase activity.4. The recombinant microbial cell of claim 3 , wherein the ACoS enzyme comprises an amino acid sequence that is at least 90% identical to SEQ ID NO:44 or 49.5. The recombinant microbial cell of claim 1 , wherein the engineered LCDA production pathway further comprises one or more of the following features:(i) up-regulation of a polynucleotide sequence encoding a cytochrome P450 monooxygenase (CYP enzyme)(ii) up-regulation of a polynucleotide sequence encoding a cytochrome P450 reductase (CPR enzyme),(iii) up-regulation of a polynucleotide sequence encoding a fatty alcohol oxidase (FAO enzyme),(iv) up-regulation of a polynucleotide sequence encoding a fatty alcohol dehydrogenase (FADH enzyme), and/or(v) up-regulation of a polynucleotide sequence encoding a fatty aldehyde dehydrogenase (FALDH enzyme).6. The recombinant microbial cell of claim 5 , wherein either ...

Methods of monitoring and modulating LKB1 activity and its downstream targets

The invention relates to methods for assaying LKB1 activity comprising providing a sample comprising LKB1, contacting the sample with a substrate kinase under conditions that permit phosphorylation, and monitoring incorporation of phosphate into the substrate kinase, wherein the incorporation of phosphate into the substrate kinase indicates LKB1 activity. Preferably the substrate kinase is or is derived from AMPK. The invention also relates to use of AMPK in the treatment or prevention of certain disorders, and to the use of AMPK in the manufacture of medicaments for certain disorders, and to the use of LKB1 in the activation and phosphorylation of AMPK. The invention also embraces yeast cells with certain genetic alterations relating to AMPKK and AMPK.

Polyalkylaromaticsilsesquioxane and preparation method thereof

The present invention relates to a highly regular and well-define structured polyorganosilsesquioxane with superior heat resistance, combustion resistance and flexibility, wherein two kinds of substituents are bonded alternately, and the preparation method thereof. The present invention provides polyorganosilsesquioxane with superior heat resistance, combustibility and flexibility. The polyorganosilsesquioxane prepared from the present invention has small molecular weight distribution and high molecular weight, and is soluble in organic solvent because a three-dimensional network structure is not formed during the condensation polymerization, which is resulted from the structure defect and randomness of the oligomer as a starting material for polymerization. Polyalkylaromaticsilsesquioxane obtained from the preparing method of the present invention is a useful heat-resistant material. It can be used for heat-resistant coating agent, protective coating agent of optical fiber, coating material ...

GENETIC TARGETING IN NON-CONVENTIONAL YEAST USING AN RNA-GUIDED ENDONUCLEASE

Non-conventional yeasts are disclosed herein comprising at least one RNA-guided endonuclease (RGEN) comprising at least one RNA component that does not have a 5′-cap. This uncapped RNA component comprises a sequence complementary to a target site sequence in a chromosome or episome in the yeast. The RGEN can bind to, and optionally cleave, one or both DNA strands at the target site sequence. An example of an RGEN herein is a complex of a Cas9 protein with a guide RNA. A ribozyme is used in certain embodiments to provide an RNA component lacking a 5′-cap. Further disclosed are methods of genetic targeting in non-conventional yeast. 1. A non-conventional yeast comprising at least one RNA-guided endonuclease (RGEN) comprising at least one RNA component that does not have a 5′-cap , wherein the RNA component comprises a sequence complementary to a target site sequence on a chromosome or episome in the yeast , wherein the RGEN can bind to the target site sequence.2. The non-conventional yeast of claim 1 , wherein the RGEN can bind to and cleave the target site sequence.3Yarrowia, Pichia, Schwanniomyces, Kluyveromyces, Arxula, Trichosporon, Candida, Ustilago, Torulopsis, Zygosaccharomyces, Trigonopsis, Cryptococcus, Rhodotorula, Phaffia, SporobolomycesPachysolen.. The non-conventional yeast of claim 1 , wherein said yeast is a member of a genus selected from the group consisting of claim 1 , and4. The non-conventional yeast of claim 1 , wherein the RGEN comprises a CRISPR (clustered regularly interspaced short palindromic repeats)-associated (Cas) protein-9 (Cas9) amino acid sequence.5. A non-conventional yeast comprising a Cas endonuclease and a polynucleotide sequence comprising a promoter operably linked to at least one nucleotide sequence claim 1 , wherein said nucleotide sequence comprises a DNA sequence encoding a ribozyme upstream of a DNA sequence encoding an RNA component claim 1 , wherein said RNA component comprises a variable targeting domain complementary to ...

METHOD AND APPARATUS FOR TRANSCEIVING FOR BEAM FORMING IN WIRELESS COMMUNICATION SYSTEM

An electronic device for beamforming and a method thereof in a wireless communication system are provided. The electronic device includes a plurality of antennas. The electronic device also includes a plurality of transmitter and receiver switches connected to the antennas and configured to select a plurality of transmission paths and a plurality of reception paths. The electronic device further includes a plurality of first Phase Shifters (P/Ss) configured to shift a phase of Radio Frequency (RF) signals received via the antennas and the transmitter and receiver switches. The electronic device includes a combiner configured to combine the phase-shifted RF signals to one RF signal. The electronic device also includes a quadrature signal generator configured to generate a quadrature signal. The electronic device further includes a down-mixer configured to convert the quadrature signal and the combined RF signal to a first baseband signal and configured to output the first baseband signal to a modem. The electronic device includes a controller configured to control the transmitter and receiver switches, the first P/Ss, and a plurality of second P/Ss to determine a transmission or reception mode of the transmitter and receiver switches, and the phase of the RF signals transmitted and received. 1. An electronic device for beamforming in a wireless communication system , comprising:a plurality of antennas;a plurality of transmitter and receiver switches connected to the plurality of antennas and configured to select a plurality of transmission paths and a plurality of reception paths;a plurality of first Phase Shifters (P/Ss) configured to shift a phase of Radio Frequency (RF) signals received via the plurality of antennas and the transmitter and receiver switches;a combiner configured to combine the phase-shifted RF signals to one RF signal;a quadrature signal generator configured to generate a quadrature signal;a down-mixer configured to convert the quadrature signal ...

Obligately symbiotic thermophile Symbiobacterium toebii SC-1 producing thermostable L-tyrosine phenol-lyase and L-tryptophan indole-lyase

The present invention relates to a novel obligately symbiotic thermophile Symbiobacterium toebii SC-1 (Accession NO: KCTC 0685BP), and a thermostable L-tyrosine phenol-lyase and L-tryptophan indole-lyase produced by Symbiobacterium toebii SC-1. The present invention also relates to a pure culture method of Symbiobacterium toebii SC-1, a method of growth measurement for Symbiobacterium toebii SC-1 and its relative symbiotic bacteria showing low-growth yield using nitrate respiration, and a screening method of relative symbiotic strains of Symbiobacterium toebii SC-1 from the environment using a specific antibody to it. Therefore, the thermostable L-tyrosine phenol-lyase and L-tryptophan indole-lyase produced by Symbiobacterium toebii SC-1 in this invention can provide more stable catalysts in enzymatic biotransformation processes which enzymatically produce valuable medico-amino acids 3,4-dehydroxy-L-phenylalanine (L-DOPA) and L-tryptophan.

Recombinant microbial cells that produce at least 28% eicosapentaenoic acid as dry cell weight

Recombinant microbial cells are disclosed herein that produce an oil comprising at least 28 percent eicosapentaenoic acid (EPA) measured as a weight percent of dry cell weight. These cells may comprise a polynucleotide sequence encoding an active acyl-CoA:lysophosphatidylcholine acyltransferase (LPCAT) comprising at least one amino acid mutation in a membrane-bound O-acyltransferase motif. In addition, the cells may comprise a down-regulation of an endogenous polynucleotide sequence encoding Sou2 sorbitol utilization protein, and/or one or more polynucleotides encoding phospholipid:diacylglycerol acyltransferase (PDAT), delta-12 desaturase, a dihomo-gamma-linolenic acid (DGLA) synthase multizyme, delta-8 desaturase, malonyl-CoA synthetase (MCS), or acyl-CoA:lysophosphatidic acid acyltransferase (LPAAT). Also disclosed are methods of using the recombinant microbial cells to produce oil containing omega-3 polyunsaturated fatty acids such as EPA.

OPTIMIZED STRAINS OF YARROWIA LIPOLYTICA FOR HIGH EICOSAPENTAENOIC ACID PRODUCTION

Described are engineered strains of the oleaginous yeast Yarrowia lipolytica capable of producing an oil comprising greater than 50 weight percent of eicosapentaenoic acid [“EPA”], an ω-3 polyunsaturated fatty acid, measured as a weight percent of total fatty acids [“% TFAs”] and having a ratio of at least 3.1 of EPA % TFAs, to linoleic acid, measured as % TFAs. These strains over-express at least one Δ9 elongase/Δ8 desaturase multizyme, in addition to other heterologous Δ9 elongases, Δ8 desaturases, Δ5 desaturases, Δ17 desaturases, Δ12 desaturases, C16/18 elongases, and optionally over-express diacylglycerol cholinephosphotransferases, malonyl CoA synthetases and/or acyl-CoA lysophospholipid acyltransferases. The expression of at least one peroxisome biogenesis factor protein is down-regulated. Methods for producing EPA within said host cells, oils obtained from the cells, and products therefrom are claimed.

Surface expression method of peptides P5 and Anal3 using the gene encoding poly-gamma-glutamate synthetase

The present invention relates to a method for expressing each of peptide antibiotics P5 3 and Anal3 35 having amphiphilicity and showing antibacterial, antifungal and anticancer activities 61, 63, 65, 67, 69, 71, on the microbial surface, using a vector containing outer membrane protein genes (pgsBCA) that are derived from Bacillus sp. strains and involved in the synthesis of poly-gamma-glutamate. Moreover, the present invention relates to lactic acid-forming bacteria having each of the peptide antibiotics P5 15 and Anal3 43 expressed on their surface, and the use thereof. According to the present invention, the peptide antibiotics can be expressed on the surface of various microorganisms transformed with the surface expression vectors. The inventive method for the surface expression of the peptide antibiotics allows the peptide antibiotics to be mass-produced without a purification process. Thus, the inventive method has very high industrial applicability. Further, the present invention ...

Grill lighting lamp system with stepped reflection and invalid portions

The present disclosure relates to a grill lighting lamp system and includes a grill part provided in a vehicle and an optical device configured to irradiate light to the grill part. The grill part includes a plurality of reflection portions configured to reflect the light irradiated from the optical device toward the outside of the vehicle and an invalid portion provided in a stepped shape between the plurality of reflection portions and configured to absorb glare light not reflected forward among light incident on the reflection portion from the optical device. According to the present disclosure, it is possible to minimize damage to the optical device in the event of a front collision of the vehicle and suppress a glare phenomenon from occurring upon grill lighting.

Rotation center decoupling type aiming lamp and vehicle including the aiming lamp

A rotation center decoupling type aiming lamp applied to a vehicle includes an aiming device in which an aiming axis spaced apart from an aiming point reference line of an aiming point axis at a distance of an aiming adjustment height is operated as a rotation center of the reflector, and an aiming angle is changed through an aiming point axis to which the reflector is attached by a reflector bracket, such that the rotation center of the reflector adjusting a light radiation direction may be formed at a position above or ahead of the aiming point reference line, thereby reducing an occupation space of the aiming device and securing stability of the aiming driving, and particularly, a sufficient rear shock absorption space may also be secured in the aiming lamp, thereby securing structural robustness while satisfying low-speed collision regulations.

Poly-gamma-glutamate having ultra high molecular weight and method for using the same

The present invention relates to a poly-gamma-glutamate (PGA) having an ultra-high molecular weight greater than 5,000 kDa. The ultra-high molecular weight PGA according to the present invention has a mean molecular weight greater than 13,000 kDa, and more than 95% of its molecules have a molecular weight ranging from 3,000 to 15,000 kDa. Also, it can be produced by the culturing of Bacillus subtilis var. chungkookjang. The ultra-high molecular weight PGA according to the present invention shows very excellent moisture-absorbing, moisture-retaining, sustained release, mineral solubility, and water-absorbing properties, and thus, can be used as a new and high value-added material in various applications.

HIDDEN LIGHTING LAMP USING COLOR CONVERSION MATERIALS AND VEHICLES HAVING SAME

A hidden lighting lamp uses a color conversion material and is applied to a vehicle. A color conversion member is spaced apart from a light source by a light source air gap (La) such that the color conversion member is integrally engaged with a lens for projecting light of the light source to the outside or such that the color conversion member is spaced apart from the lens by a lens air gap (Lb). Hidden lighting without deterioration of optical efficiency of LED light from the light source is thereby implemented. One or more of a black painting, a coating layer, an application layer, and a deposition layer are provided, thereby providing concealment of the lamp inner space of the lens, anti-peeling of a deposit, and diversity of fluorescent color.

PENTOSE PHOSPHATE PATHWAY UPREGULATION TO INCREASE PRODUCTION OF NON-NATIVE PRODUCTS OF INTEREST IN TRANSGENIC MICROORGANISMS

Coordinately regulated over-expression of the genes encoding glucose 6-phosphate dehydrogenase [G6PDH] and 6-phospho-gluconolactonase [6PGL] in transgenic strains of the oleaginous yeast, Yarrowia lipolytica, comprising a functional polyunsaturated fatty acid [PUFA] biosynthetic pathway, resulted in increased production of PUFAs and increased total lipid content in the Yarrowia cells. This is achieved by increased cellular availability of the reduced form of nicotinamide adenine dinucleotide phosphate [NADPH], an important reducing equivalent for reductive biosynthetic reactions, within the transgenic microorganism.

MANIPULATION OF SNF1 KINASE FOR ALTERED OIL CONTENT IN OLEAGINOUS ORGANISMS

Methods of increasing the total lipid content in a eukaryotic cell, the total content of polyunsaturated fatty acids [PUFAs], and/or the ratio of desaturated fatty acids to saturated fatty acids by reducing the activity of the heterotrimeric SNF1 protein kinase are disclosed. Preferably, the chromosomal genes encoding the Snf1 -subunit, Gal83 -subunit or Snf4 -subunit of the SNF1 protein kinase, the upstream regulatory genes encoding Sak1, Hxk2, Glk1 or Reg1, or the downstream genes encoding Rme1, Cbr1 or Snf3 are manipulated in a PUFA-producing strain of the oleaginous yeast Yarrowia lipolytica, resulting in increased total lipid content, as compared to the parent strain comprising the heterotrimeric SNF1 protein kinase not having reduced activity.

Optimized strains of yarrowia lipolytica for high eicosapentaenoic acid production

Described are engineered strains of the oleaginous yeast Yarrowia lipolytica capable of producing an oil comprising greater than 50 weight percent of eicosapentaenoic acid [“EPA”], an ω-3 polyunsaturated fatty acid, measured as a weight percent of total fatty acids [“% TFAs”] and having a ratio of at least 3.1 of EPA % TFAs, to linoleic acid, measured as % TFAs. These strains over-express at least one Δ9 elongase/Δ8 desaturase multizyme, in addition to other heterologous Δ9 elongases, Δ8 desaturases, Δ5 desaturases, Δ17 desaturases, Δ12 desaturases, C 16/18 elongases, and optionally over-express diacylglycerol cholinephosphotransferases, malonyl CoA synthetases and/or acyl-CoA lysophospholipid acyltransferases. The expression of at least one peroxisome biogenesis factor protein is down-regulated. Methods for producing EPA within said host cells, oils obtained from the cells, and products therefrom are claimed.

Vehicle lamp with rotating light source where the light sources are rotated based on sensed image

A vehicle lamp with a rotating light source is provided. The lamp includes a signal transmitter that receives a signal from one or more sensors provided in a vehicle and an LED unit having one or more LED elements for irradiating light to the outside of the vehicle. A controller receives the signal from the signal transmitter. A power transmitter and a power receiver receive an applied voltage to be applied to the one or more LED elements from the controller, and apply the applied voltage to each of the one or more LED elements, and a driver that rotates the LED unit. The controller determines an image shape according to the signal, and calculates the applied voltage to be applied to the one or more LED elements according to the computed image shape.

HIDDEN LIGHTING FILM LENS FORMATION APPARATUS AND HIDDEN LIGHTING FILM LENS

Proposed is a hidden lighting lens formation apparatus. In a film formation process, a transmissivity-adjustable film having a flange whose rear surface is fixed to a top portion overlapping a lens hidden lighting section, an end portion of a both-end portion rear surface, and a fixation hole is manufactured. In an injection formation process, the film is fixed with a cavity sidewall, a film insertion portion, and a protrusion in a cavity in a mold. A lens and a bezel are formed by injecting resin through injection molding. As a result, the film absorbs film-forming dispersion for high-frequency formation. Additionally, when a lens, together with the film, is formed through insertion injection, a desired pattern can be formed without causing an inconsistent shape of an end portion of the film in the cavity in the mold and pushing the end portion of the film.

Polyalkylaromaticsilsesquioxane and preparation method thereof

The present invention relates to a highly regular and well-defined ladder structured polyorganosilsesquioxane with superior heat resistance, combustion resistance and flexibility. This product is made by alternatingly bonding together two kinds of substituents. The polyorganosilsesquioxane prepared according to the present invention has a small molecular weight distribution and a high molecular weight. It is soluble in organic solvents because its three-dimensional network structure is not formed as a result of a condensation polymerization, which resulted in a product having structure defect(s) and randomness because of the oligomer that was previously used as a starting material for polymerization. Polyalkylaromaticsilsesquioxanes obtained according to the preparing method of the present invention are useful, heat-resistant materials. They can be used as a heat-resistant coating agent, an agent for protecting optical fiber, a coating material for a resistor, heat-resistant paint, adhesive ...

Surface expression method of peptides P5 and Anal3 using the gene encoding poly-gamma-glutamate synthetase

The present invention relates to a method for expressing each of peptide antibiotics P5 3 and Ana13 35 having amphiphilicity and showing antibacterial, antifungal and anticancer activities 61, 63, 65, 67, 69, 71, on the microbial surface, using a vector containing outer membrane protein genes (pgsBCA) that are derived from Bacillus sp. strains and involved in the synthesis of poly-gamma-glutamate. Moreover, the present invention relates to lactic acid-forming bacteria having each of the peptide antibiotics P5 15 and Ana13 43 expressed on their surface, and the use thereof. According to the present invention, the peptide antibiotics can be expressed on the surface of various microorganisms transformed with the surface expression vectors. The inventive method for the surface expression of the peptide antibiotics allows the peptide antibiotics to be mass-produced without a purification process. Thus, the inventive method has very high industrial applicability. Further, the present invention ...

ROTATION CENTER DECOUPLING TYPE AIMING LAMP AND VEHICLE INCLUDING THE AIMING LAMP

A rotation center decoupling type aiming lamp applied to a vehicle includes an aiming device in which an aiming axis spaced apart from an aiming point reference line of an aiming point axis at a distance of an aiming adjustment height is operated as a rotation center of the reflector, and an aiming angle is changed through an aiming point axis to which the reflector is attached by a reflector bracket, such that the rotation center of the reflector adjusting a light radiation direction may be formed at a position above or ahead of the aiming point reference line, thereby reducing an occupation space of the aiming device and securing stability of the aiming driving, and particularly, a sufficient rear shock absorption space may also be secured in the aiming lamp, thereby securing structural robustness while satisfying low-speed collision regulations.

METHOD FOR PERSONALIZING VEHICLE LAMPS

A method of personalizing a vehicle lamp includes drawing an image made up of a plurality of pixels that implement a vehicle lamp, based on an input by a user. The method also includes converting the drawn image into light-and-shade data that correspond to the pixels, respectively, multiplying a pixel light distribution value, corresponding to each pixel, by a weighted value based on the data resulting from the conversion, adding up results of the multiplication, and deriving total light distribution of the pixels that results when brightness of the vehicle lamp is the same as that of the drawn image. The method further includes comparing the derived total light distribution against a predetermined reference value and determining whether the derived total light distribution satisfies a predetermined light distribution reference, and applying the drawn image to the vehicle lamp when it is determined that a predetermined light distribution reference is satisfied.

Manipulation of SNF1 kinase for altered oil content in oleaginous organisms

Methods of increasing the total lipid content in a eukaryotic cell, the total content of polyunsaturated fatty acids ["PUFAs"], and/or the ratio of desaturated fatty acids to saturated fatty acids by reducing the activity of the heterotrimeric SNF1 protein kinase are disclosed. Preferably, the chromosomal genes encoding the Snf1 alpha-subunit, Gal83 beta-subunit or Snf4 gamma-subunit of the SNF1 protein kinase, the upstream regulatory genes encoding Sak1, Hxk2, Glk1 or Reg1, or the downstream genes encoding Rme1, Cbr1 or Snf3 are manipulated in a PUFA-producing strain of the oleaginous yeast Yarrowia lipolytica, resulting in increased total lipid content, as compared to the parent strain comprising the heterotrimeric SNF1 protein kinase not having reduced activity.

Rotation light source device and lamp system thereof

A lamp system applied to a vehicle according to the present disclosure is provided with any one of a reflector, an optical member, a digital micromirror display (DMD), a shield, or a shield optical module, and combined with a rotation light source device for generating light of a specific LED turned on at a synchronized rotation position of one or more LED chips of first to Nth LED chips (N is an integer of 2 or more) per one rotation while being rotated by a current application of a signal transmitter receiving a lamp turn-on signal of the vehicle, thereby generating various lighting patterns even while eliminating all problems of increasing the layout/decreasing the light amount/increasing the amount of property changed, lowering the reflection efficiency/transmission efficiency, and losing the optical efficiency with the circular LED array.

NOVEL CONSTITUTIVE STRONG PROMOTER AND USE THEREOF

Disclosed herein are an aldolase gene promoter and the use thereof, more particularly, disclosed are a promoter of aldolase gene derived from Lactobacillus casei, having a base sequence of SEQ ID NO: 1, an expression vector containing said promoter, and a recombinant microorganism transformed with said expression vector. The recombinant microorganism transformed with the expression vector containing the disclosed promoter can effectively express a target protein on the surface thereof, and thus will be useful as vaccine vehicles and the like.

Method and apparatus for processing signal in a mobile device

The present disclosure relates to a sensor network, Machine Type Communication (MTC), Machine-to-Machine (M2M) communication, and technology for Internet of Things (IoT). The present disclosure may be applied to intelligent services based on the above technologies, such as smart home, smart building, smart city, smart car, connected car, health care, digital education, smart retail, security and safety services. A method and apparatus for processing a signal in a mobile device are provided. The method includes classifying signals transmitted and received between devices according to at least two predetermined rates, and transmitting and receiving the classified signals in connection lines supporting the at least two rates, respectively.

ANTICOAGULANT AND COMPOSITION FOR PREVENTING THROMBUS CONTAINING POLY-GAMMA-GLUTAMIC ACID

The present invention relates to an anticoagulant and a composition for preventing thrombus formation, which contain poly-gamma-glutamic acid (PGA) as an active ingredient. The inventive PGA is a water-soluble, anionic, biodegradable and edible amino acid polymer material, which has an anticoagulant effect of preventing thrombi from being accumulated in blood vessels, shows an excellent sustained-release effect and is harmless to the human body. Thus, it is useful as a high-value-added anticoagulant and a food or beverage composition for preventing thrombus formation. 16.-. (canceled)7. A method of inhibiting blood coagulation comprising administering to a patient in need thereof an anticoagulant comprising a therapeutically effective amount of poly-gamma-glutamic acid (PGA) as an active ingredient.8. The method accord to claim 7 , wherein the molecular weight of said poly-gamma-glutamic acid (PGA) is 1-15 claim 7 ,000 kDa.9. A method of preventing thrombus formation comprising administering to a patient in need thereof a pharmaceutical composition comprising a therapeutically effective amount of poly-gamma-glutamic acid (PGA) as an active ingredient.10. The method according to claim 9 , wherein the molecular weight of said poly-gamma-glutamic acid is 1-15 claim 9 ,000 kDa.11. A method of preventing thrombus formation by using a food comprising a therapeutically effective amount of poly-gamma-glutamic acid (PGA) as an active ingredient.12. The method according to claim 11 , wherein the molecular weight of said poly-gamma-glutamic acid is 1-15 claim 11 ,000 kDa.13. The method according to claim 11 , wherein said food is green tea or brown rice green tea. The present invention relates to an anticoagulant and a composition for preventing thrombus formation, which contain poly-gamma-glutamic acid (PGA) as an active ingredient.Blood coagulation is a cascade of hemostatic events during which blood changes from a liquid state to a gel state. Hemostatic events in the body ...

YARROWIA PEROXISOMAL 2,4-DIENOYL-COA REDUCTASE PROMOTER REGIONS FOR GENE EXPRESSION IN YEAST

Promoter regions associated with the peroxisomal 2,4-dienoyl-CoA reductase (SPS19) gene are disclosed and have been found to be particularly effective for the expression of heterologous genes in yeast. These promoter regions will be useful for driving high-level expression of genes involved in the production of omega-3 and omega-6 fatty acids. 1. A method for the expression of a coding region of interest in a transformed yeast cell comprising: [{'i': 'Yarrowia', '(1) a promoter region of a SPS19 gene; and'}, '(2) a coding region of interest which is expressible in the yeast cell;, 'wherein the recombinant construct comprises, 'a) providing a transformed yeast cell having a recombinant construct,'}wherein the promoter region is operably linked to the coding region of interest; andb) growing the transformed yeast cell of step (a) under conditions whereby the recombinant construct of step (a) is expressed.2Yarrowia. The method according to claim 1 , wherein the promoter region of the SPS19 gene comprises a sequence selected from the group consisting of SEQ ID NO:39 and SEQ ID NO:40.3Yarrowia. The method according to claim 1 , wherein the promoter region of the SPS19 gene comprises SEQ ID NO:5 claim 1 , wherein said promoter region optionally comprises at least one modification selected from the group consisting of:{'b': '1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154 ...

Water-Soluble Polypeptides Comprised of Repeat Modules, Method for Preparing the Same and Method for a Target-Specific Polypeptide and Analysis of Biological Activity Thereof

The present invention relates to a soluble polypeptide comprised of repeat modules. More particularly, the present invention relates to a soluble fusion polypeptide of the N-terminal domain of internalin and LRR (Leucine rich repeat) family protein, a method for preparing the polypeptide, a vector comprising a nucleic acid sequence encoding the polypeptide, a host cell comprising the vector, a method for producing a solubility and folding-improved fusion polypeptide by expressing the vector in the host cell, and a method for improving the solubility and folding of the fusion polypeptide. Further, the present invention relates to a method for preparing the polypeptide bound with a specific target and analyzing the efficacy of the soluble polypeptide. 1. A soluble fusion polypeptide prepared by fusion of the N-terminal domain of internalin protein and LRR (Leucine-rich repeat) family protein.2. The fusion polypeptide according to claim 1 , wherein the internalin protein is selected from the group consisting of internalin A claim 1 , internalin B claim 1 , internalin C claim 1 , internalin H and internalin J.3. The fusion polypeptide according to claim 1 , wherein the LRR family protein is selected from the group consisting of Variable lymphocyte repeat (VLR) claim 1 , Toll-like receptor (TLR) claim 1 , TV3 claim 1 , U2A and Ribonuclease Inhibitor (RI).4. The fusion polypeptide according to claim 1 , wherein the LRR family protein has 1 to 9 LRR repeat modules.5. The fusion polypeptide according to claim 1 , wherein the N-terminal domain of internalin protein and the LRR family protein are linked via a linker.6. The fusion polypeptide according to claim 5 , wherein the linker is a VLR repeat module.7. The fusion polypeptide according to claim 1 , wherein the LRR family protein is modified by substitution claim 1 , deletion or insertion of amino acids claim 1 , in order that the fusion polypeptide is capable of binding with target protein.8. The fusion polypeptide ...

METHOD AND DEVICE FOR EXTENDING BEAM AREA IN WIRELESS COMMUNICATION SYSTEM

The present disclosure relates to a pre-5th-generation (5G) or 5G communication system to be provided for supporting higher data rates beyond 4th-generation (4G) communication system such as long term evolution (LTE). An electronic device is provided in a wireless communication system. The device comprises a plurality of antenna sets; a plurality of antenna elements configuring the plurality of antenna sets; an RF transceiver including a plurality of switches for selecting the plurality of antenna elements and a plurality of phase shifters for shifting the phase of a signal transmitted/received through the plurality of antenna elements; and a control unit for determining a beam forming direction and the phase of the signal by simultaneously controlling the plurality of switches and the plurality of phase shifters according to a beambook. 1. An electronic device in a wireless communication system , the device comprising:a plurality of antenna sets consisting of a combination of a plurality of antenna elements;a plurality of switches for selecting the plurality of antenna elements;a radio frequency (RF) transceiver comprising a plurality of phase shifters for shifting a phase of a signal transmitted/received through the plurality of antenna elements; andat least one processor for determining a beamforming direction and the phase of the signal by simultaneously controlling the plurality of switches and the plurality of phase shifters.2. The device of claim 1 , wherein the plurality of antenna sets and the plurality of antenna elements are integrated on a multi-layer substrate.3. The device of claim 2 , wherein the multi-layer substrate has sections A claim 2 , B claim 2 , and C configured in a row.4. The device of claim 3 , wherein the plurality of antenna sets and the plurality of antenna elements comprise at least one of a broadside antenna and an end-fire antenna.5. The device of claim 3 , wherein the plurality of antenna sets and the plurality of antenna elements ...

PEROXISOME BIOGENESIS FACTOR PROTEIN (PEX) DISRUPTIONS FOR ALTERING POLYUNSATURATED FATTY ACIDS AND TOTAL LIPID CONTENT IN OLEAGINOUS EUKARYOTIC ORGANISMS

Methods of increasing the amount of polyunsaturated fatty acids (PUFAs) in the total lipid fraction and in the oil fraction of PUFA-producing, oleaginous eukaryotes, accomplished by modifying the activity of peroxisome biogenesis factor (Pex) proteins. Disruptions of a chromosomal Pex3 gene, Pex10p gene or Pex16p gene in a PUFA-producing, oleaginous eukaryotic strain resulted in an increased amount of PUFAs, as a percent of total fatty acids and as a percent of dry cell weight, in the total lipid fraction and in the oil fraction of the strain, as compared to the parental strain whose native Pex protein was not disrupted. 1. A method of increasing the weight percent of at least one polyunsaturated fatty acid relative to the weight percent of total fatty acids in an oleaginous eukaryotic organism having a total lipid content , a total lipid fraction and an oil fraction , comprising: 1) genes encoding a functional polyunsaturated fatty acid biosynthetic pathway; and', '2) a disruption in a native gene encoding a peroxisome biogenesis factor protein, thereby providing a PEX-disrupted organism, and, 'a) providing an oleaginous eukaryotic organism comprisingb) growing the PEX-disrupted organism under conditions as to increase the weight percent of at least one polyunsaturated fatty acid relative to the weight percent of total fatty acids in the total lipid fraction or in the oil fraction, when compared to the weight percent of the at least one polyunsaturated fatty acid relative to the weight percent of total fatty acids in the total lipid fraction or in the oil fraction in the oleaginous eukaryotic organism in which no native gene encoding a peroxisome biogenesis factor protein has been disrupted.2. The method of claim 1 , wherein the increase in the weight percent of the at least one polyunsaturated fatty acid relative to the weight percent of total fatty acids is at least 1.3 fold claim 1 , when compared to the weight percent of polyunsaturated fatty acids relative to ...

VEHICLE LAMP WITH ROTATING LIGHT SOURCE

A vehicle lamp with a rotating light source may include a signal receiver which receives a signal from one or more sensors provided in a vehicle; a light emitting diode (LED) portion having one or more LED elements configured to emit light toward an outside of the vehicle; a controller connected to the one or more LED elements and configured to control a light generation amount of the one or more LED elements; a signal transmitter which is connected to the signal receiver, receives the signal from the signal receiver and transmits the received signal to the controller; and a driver coupled to the LED portion and configured to rotate the LED portion, wherein the controller controls the light generation amount of the one or more LED elements in a response to the signal. 1. A vehicle lamp apparatus with a rotating light source , the vehicle lamp apparatus comprising:a signal receiver which is connected to one or more sensors mounted in a vehicle and receives a signal from the one or more sensors;a light emitting diode (LED) portion having one or more LED elements configured to emit light toward an outside of the vehicle;a controller connected to the one or more LED elements and configured to control a light generation amount of the one or more LED elements;a signal transmitter which is connected to the signal receiver, receives the signal from the signal receiver and transmits the received signal to the controller; anda driver coupled to the LED portion and configured to rotate the LED portion,wherein the controller is configured to control the light generation amount of the one or more LED elements in a response to the received signal.2. The vehicle lamp apparatus of claim 1 , whereinthe controller is disposed on a rear surface of the LED portion;the LED portion is fixed to a rotation shaft which protrudes from the driver toward the outside of the vehicle;the signal transmitter is configured to transmit the signal to the controller through the rotation shaft;the ...

LAMP DEVICE FOR VEHICLE

A lamp device applied to a vehicle may include a flow change generation device which accelerates an air flow in a space around a heat sink dissipating the heat due to the radiation of the light of a light source of an optical module to form the flow enhancing heat-dissipation efficiency of the heat sink, and generates the flow by the shake due to any one of an inertia force of a vehicle, a magnetic force, and a combination of the inertial force of the vehicle and the magnetic force, enhancing heat-dissipation efficiency by the heat dissipation using a change in the flow around a heat source together with the heat dissipation due to the heat sink. 1. A lamp apparatus comprising:an optical module having a light source of radiating light;a heat sink for dissipating a heat caused by the light source, by an air flow; anda flow change generation device generating a flowage of a fluid by a flowage movement of the fluid due to an inertia force of a vehicle, and dispersing the heat into the air flow; andan electromagnet, wherein the flow change generation device causes the flowage movement by a magnetic force of the electromagnet by supplying a power from a power source to the electromagnet.2. The lamp apparatus of claim 1 ,wherein the fluid includes a first fluid and a second fluid,wherein the flow change generation device includes a vibration box having the first fluid and the second fluid having different densities and filled in the vibration box, andwherein the first fluid and the second fluid form the flowage of the fluid in the vibration box due to the inertia force of the vehicle to cause a flowage movement of the fluid in the vibration box.3. The lamp apparatus of claim 2 ,wherein the first fluid is made of a rarer material, and the second fluid is made of a denser material to form a density difference between the first fluid and the second fluid.4. The lamp apparatus of claim 3 ,wherein the first fluid is one of air, benzene, and toluene, and the second fluid is ...

METHOD FOR OUTPUTTING SCREEN ACCORDING TO FORCE INPUT AND ELECTRONIC DEVICE SUPPORTING THE SAME

An electronic device is provided that includes a memory, a display that detects a force input based on an external pressure, and a processor electrically coupled with the memory and the display. The processor executes an application that is operable corresponding to the force input, controls to store history information in the memory on use of the electronic device, the history information being related to a forcing point, to which the force input is applied, on an execution screen of the application, and controls to output via the display the history information to an area adjacent to the forcing point if the force input to the forcing point occurs. 1. An electronic device comprising:a memory;a display configured to detect a force input based on an external pressure; and execute an application that is operable corresponding to the force input;', 'control to store history information in the memory on use of the electronic device, wherein the history information is related to a forcing point, to which the force input is applied, on an execution screen of the application; and', 'control to output via the display the history information to an area adjacent to the forcing point if the force input to the forcing point occurs., 'a processor electrically coupled with the memory and the display, wherein the processor is configured to2. The electronic device of claim 1 , wherein the processor is configured to control to start storing the history information in the memory if the application starts.3. The electronic device of claim 1 , wherein the forcing point is a control button displayed on the display while the application is executed.4. The electronic device of claim 3 , wherein the processor is configured to control to output a shortcut claim 3 , which is used to execute at least some of a plurality of functions executable through the control button claim 3 , via the display to the area.5. The electronic device of claim 4 , wherein the processor determines the some of ...

REFLECTOR FOR VEHICLE LAMPS

A reflector device configured for vehicle lamps which is configured for causing light to exit along a plurality of paths using a single light source and a single reflector, may include a fluid, the position of which is changed in a response to magnetism, is provided at the reflector such that the exit path of light is changed depending on the flow of the fluid, whereby it is possible to realize a lamp having various functions. 1. A reflector device for vehicle lamps , the reflector device comprising:a light source unit for emitting light;a variable reflector having therein a reaction fluid that reacts to magnetism and a non-reaction fluid that does not react to the magnetism,wherein the variable reflector further includes a first portion located in a movement path of the light emitted from the light source unit and a second portion located to deviate from the movement path of the light, andwherein the first portion located in the movement path of the light is configured to reflect incident light such that the light of the light source unit exits to an outside of the reflector device; andan electromagnet unit mounted at the variable reflector for selectively generating the magnetism to control a fluid flow of the reaction fluid such that the reaction fluid or the non-reaction fluid is selectively disposed in the movement path of the light, whereby the light of the light source unit is reflected by one of the reaction fluid and the non-reaction fluid or is transmitted through another of the reaction fluid and the non-reaction fluid and then reflected by the variable reflector to adjust an exit direction of the light, a bent reflection unit located in the movement path of the light for reflecting the light; and', 'a reception unit formed to fluidically-communicate with the bent reflection unit, the reception unit extending to deviate from the movement path of the light, and, 'wherein the variable reflector includeswherein the electromagnet unit is disposed at an ...

PEPTIDE-MEDIATED DELIVERY OF RNA-GUIDED ENDONUCLEASE INTO CELLS

A composition is disclosed that comprises at least one protein component of an RNA-guided endonuclease (RGEN) and at least one cell-penetrating peptide (CPP), wherein the RGEN protein component and CPP are covalently or non-covalently linked to each other in an RGEN protein-CPP complex. The RGEN protein-CPP complex can traverse (i) a cell membrane, or (ii) a cell wall and cell membrane, of a cell. The RGEN protein component of an RGEN protein-CPP complex in certain embodiments can be associated with a suitable RNA component to provide an RGEN capable of specific DNA targeting. Further disclosed are compositions comprising at least one protein component of a guide polynucleotide/Cas endonuclease complex and at least one CPP, as well as methods of delivering RGEN proteins into microbial cells, as well as methods of targeting DNA with RGENs. 118-. (canceled)19. A method for modifying a target site in the genome of a microbial cell , the method comprising providing a guide polynucleotide , a cell-penetrating peptide (CPP) and a Cas endonuclease to the microbial cell , wherein said guide polynucleotide , Cas endonuclease and CPP are covalently , or non-covalently , linked to each other in a guide polynucleotide/Cas endonuclease-CPP complex , and wherein said guide polynucleotide/Cas endonuclease-CPP complex can traverse (i) a cell membrane , or (ii) a cell wall and cell membrane , of the microbial cell , wherein the guide polynucleotide and CPP-Cas endonuclease are capable of forming a complex that enables the Cas endonuclease to introduce a double-strand break at the target site in the genome of the microbial cell.20. The method of claim 19 , wherein the modifying at said target site includes (i) a replacement of at least one nucleotide claim 19 , (ii) a deletion of at least one nucleotide claim 19 , (iii) an insertion of at least one nucleotide claim 19 , or (iv) any combination of (i)-(iii).21. The method of claim 19 , further comprising identifying at least one ...

COMPOSITON BASED ON BIOCOMPATIBLE ANIONIC POLYMER FOR DRUG DELIVERY AND PREPARING METHOD THEREOF

A composition for drug delivery based on a biocompatible anionic polymer and a producing method thereof are provided. 1. A composition for drug delivery , comprisinga biocompatible anionic polymer;an agonist of an immunostimulant; andan active pharmaceutical ingredient.2. The composition for drug delivery of claim 1 ,wherein the biocompatible anionic polymer includes a member selected from the group consisting of carboxyl group, hydroxyl group, sulfonic group, sulfate group, and combinations thereof.3. The composition for drug delivery of claim 1 ,wherein the biocompatible anionic polymer includes a member selected from the group consisting of a poly-gamma-glutamic acid, a hyaluronic acid, a cellulose, a polyacrylic acid, a polyamino acid, a polysaccharide, derivatives thereof, and combinations thereof.4. The composition for drug delivery of claim 1 ,wherein the immunostimulant includes a toll-like receptor, a NOD-like receptor, or a cytokine.5. The composition for drug delivery of claim 1 ,wherein the agonist of the immunostimulant includes poly(I:CU), CpG, imiquimod, resiquimod, dSLIM, MPLA, flagellin, a plasmid DNA double-strand DNA, a single-strand DNA, a saponin, or an interleukin cytokine.6. The composition for drug delivery of claim 1 ,wherein the active pharmaceutical ingredient includes an anticancer agent.7. The composition for drug delivery of claim 6 ,wherein the anticancer agent includes a member selected from the group consisting of paclitaxel, docetaxel, doxorubicin, adriamycin, cis-platin, mitomycin-C, daunomycin, 5-fluorouracil, griseofulvin, digoxin, dipyridamol, spironolactone, cyclosporine, amphotericin B, etoposide, 6-mercaptopurine, dexamethasone, perphenazine, 20-S-camptothecin, 9-nitro-camptothecin, 9-amino-camptothecin, 10,11-methylenedioxy-camptothecin, taxol, taxol-A, mitotane, methotrexate, lomustine, interferon, rouracil, and etoposide.8. The composition for drug delivery of claim 1 ,wherein the biocompatible anionic polymer, the agonist ...

APPARATUS AND METHOD FOR PROVIDING INTEGRATED DEVICE INFORMATION

An apparatus and a method for providing integrated device information are provided. The apparatus includes a communication unit that transmits and receives data through a first type of wireless communication and a second type of wireless communication; and a controller that discovers a first wireless communication device supporting the first type of wireless communication, receives first wireless communication device information from the discovered first wireless communication device, discovers a second wireless communication device supporting the second type of wireless communication, receives second wireless communication device information from the discovered second wireless communication device, integrates the first wireless communication device information and the second wireless communication device information, and displays the integrated wireless communication device information. 1. An apparatus for providing integrated device information , the apparatus comprising:a communication unit that transmits and receives data through a first type of wireless communication and a second type of wireless communication; anda controller that discovers a first wireless communication device supporting the first type of wireless communication, receives first wireless communication device information from the discovered first wireless communication device, discovers a second wireless communication device supporting the second type of wireless communication, receives second wireless communication device information from the discovered second wireless communication device, integrates the first wireless communication device information and the second wireless communication device information, and displays the integrated wireless communication device information.2. The apparatus of claim 1 , wherein the controller scans the first wireless communication device to discover the first wireless communication device claim 1 , and receives the first wireless communication device ...

DOWN-REGULATION OF A POLYNUCLEOTIDE ENCODING A SOU2 SORBITOL UTILIZATION PROTEIN TO MODIFY LIPID PRODUCTION IN MICROBIAL CELLS

Recombinant microbial cells are disclosed herein that comprise (i) a down-regulation of an endogenous polynucleotide sequence encoding Sou2 sorbitol utilization protein, and (ii) a polyunsaturated fatty acid (PUFA) biosynthetic pathway. The down-regulation of the polynucleotide sequence encoding Sou2 sorbitol utilization protein can increase the lipid content of the microbial cells and/or decrease the total amount of sugar alcohols produced by the microbial cells. Also disclosed are methods of using the recombinant microbial cells to produce oil containing omega-3 polyunsaturated fatty acids such as EPA. 1. A recombinant microbial cell comprising:(i) a down-regulation of an endogenous polynucleotide sequence encoding Sou2 sorbitol utilization protein, and(ii) a polyunsaturated fatty acid (PUFA) biosynthetic pathway,wherein said down-regulation increases the lipid content of the microbial cell and/or decreases the total amount of sugar alcohols produced by the microbial cell.2. The recombinant microbial cell of claim 1 , wherein said down-regulation is due to a mutation of said endogenous polynucleotide sequence.3. The recombinant microbial cell of claim 2 , wherein said mutation is selected from the group consisting of a substitution claim 2 , deletion and insertion.4. The recombinant microbial cell of claim 3 , wherein said mutation is a deletion that removes(i) one or more nucleotides from an open reading frame encoding said Sou2 sorbitol utilization protein, and/or(ii) one or more nucleotides of a non-protein-coding sequence located within 500 base pairs of the 5′-end of said open reading frame.5. The recombinant microbial cell of claim 3 , wherein said mutation is an insertion that occurs within(i) an open reading frame encoding said Sou2 sorbitol utilization protein, or(ii) a non-protein-coding sequence located within 500 base pairs of the 5′-end of said open reading frame.6. The recombinant microbial cell of claim 1 , wherein said PUFA pathway produces at ...

PEPTIDE-MEDIATED DELIVERY OF RNA-GUIDED ENDONUCLEASE INTO CELLS

A composition is disclosed that comprises at least one protein component of an RNA-guided endonuclease (RGEN) and at least one cell-penetrating peptide (CPP), wherein the RGEN protein component and CPP are covalently or non-covalently linked to each other in an RGEN protein-CPP complex. The RGEN protein-CPP complex can traverse (i) a cell membrane, or (ii) a cell wall and cell membrane, of a cell. The RGEN protein component of an RGEN protein-CPP complex in certain embodiments can be associated with a suitable RNA component to provide an RGEN capable of specific DNA targeting. Further disclosed are compositions comprising at least one protein component of a guide polynucleotide/Cas endonuclease complex and at least one CPP, as well as methods of delivering RGEN proteins into microbial cells, as well as methods of targeting DNA with RGENs. 1. A composition comprising at least one protein component of an RNA-guided endonuclease (RGEN) and at least one cell-penetrating peptide (CPP) ,wherein said protein component and CPP are covalently, or non-covalently, linked to each other in an RGEN protein-CPP complex, andwherein said RGEN protein-CPP complex can traverse (i) a cell membrane, or (ii) a cell wall and cell membrane, of a microbial cell.2. The composition of claim 1 , wherein the protein component of the RGEN is associated with at least one RNA component that comprises a sequence complementary to a target site sequence on a chromosome or episome in the microbial cell claim 1 , wherein the RGEN can bind to the target site sequence claim 1 , and optionally cleave one or both DNA strands at the target site sequence.3. The composition of claim 2 , wherein the RNA component comprises a guide RNA (gRNA) comprising a CRISPR RNA (crRNA) operably linked to a trans-activating CRISPR RNA (tracrRNA).4. The composition of claim 2 , wherein the RGEN can cleave one or both DNA strands at the target site sequence.5. The composition of claim 1 , wherein the RGEN comprises a CRISPR- ...

CONTENT OUTPUT SYSTEM, DISPLAY APPARATUS AND CONTROL METHOD THEREOF

A content output system is provided. The content output system includes a display apparatus configured to display a user interface (UI) for guiding a user input for setting a speaker corresponding to each of a plurality of channels according to a predetermined event and a plurality of speaker apparatuses configured to, in response to a user input corresponding to the user input guide being received, transmit a first signal corresponding to the user input to the display apparatus. 1. A content output system comprising a display apparatus and a plurality of speaker apparatuses , the system comprising:a display apparatus, including a display, configured to display a user interface (UI) configured for guiding a user input for setting a speaker corresponding to each of a plurality of channels according to a predetermined event; anda plurality of speaker apparatuses, each including a speaker, configured to, in response to a user input corresponding to the user input guide being received, transmit a signal corresponding to the user input to the display apparatus,wherein the display apparatus is configured to, in response to the signal being received from each of the plurality of speaker apparatuses, transmit an acoustic signal corresponding to a channel set in each of the plurality of speaker apparatuses.2. The content output system as claimed in claim 1 , wherein the UI is configured to guide a position of a speaker corresponding to each of the plurality of channels and guides a selection for a speaker corresponding to the plurality of channels.3. The content output system as claimed in claim 1 , wherein the display apparatus is configured to provide information relating to a multi channel type settable based on a number of a plurality of speaker apparatuses connected to the display apparatus according to the predetermined event claim 1 ,wherein the display apparatus is configured to, in response to a particular multi channel type being selected, display the UI ...

Method and Apparatus for Processing Signal in a Mobile Device

The present disclosure relates to a sensor network, Machine Type Communication (MTC), Machine-to-Machine (M2M) communication, and technology for Internet of Things (IoT). The present disclosure may be applied to intelligent services based on the above technologies, such as smart home, smart building, smart city, smart car, connected car, health care, digital education, smart retail, security and safety services. A method and apparatus for processing a signal in a mobile device are provided. The method includes classifying signals transmitted and received between devices according to at least two predetermined rates, and transmitting and receiving the classified signals in connection lines supporting the at least two rates, respectively. 1. A method for processing a signal in a mobile device , the method comprising:classifying a type of signal, as a classified signal, to one of at least two predetermined rates for communicating the classified signal between devices in the mobile device; andcommunicating the classified signal in a connection line supporting one of the at least two rates.2. The method of claim 1 , wherein the at least two rates include a first rate satisfying a predetermined condition and a second rate higher than the first rate claim 1 , and wherein the connection line supporting the first rate supports bidirectional communication.3. The method of claim 1 , wherein communicating comprises transmitting and receiving control signals in the connection line supporting a first rate.4. The method of claim 3 , further comprising a first device transmitting a switching signal indicating change in communication direction in the connection line supporting the first rate to a second device prior to the first device transmitting the classified signal on the connection line claim 3 , wherein the switching signal is transmitted by use of at least one of a separate channel and a signal having a predetermined pattern.5. The method of claim 1 , wherein at least one of ...

BACKLIGHT UNIT AND DISPLAY DEVICE HAVING THE SAME

A backlight unit includes a light source, a mold frame which accommodates the light source includes at least a first sidewall and an inclined surface protruding and sloping downward from an inner side surface of the first sidewall. A second sidewall may extend from the inclined surface in which the second sidewall is arranged substantially parallel to the first sidewall and has a space therebetween. The support member is disposed on the inclined surface of the mold frame. An optical plate may be disposed on an upper surface of the support member, and the optical plate and the inclined surface of the mold frame define an optical path in a space there between. 1. A backlight unit comprising:a light source;a mold frame which accommodates the light source, the mold frame includes at least a first sidewall;a support member disposed on the mold frame,wherein the mold frame further includes an inclined surface protruding and sloping downward from an inner side surface of the first sidewall, and the support member is disposed on the inclined surface of the mold frame.2. The backlight unit of claim 1 , wherein the support member comprises an upper surface disposed substantially perpendicular to the first sidewall of the mold frame.3. The backlight unit of claim 2 , wherein the support member includes an inclined surface sloping downward claim 2 , and the inclined surface of the support member has a same inclination angle as the inclined surface of the mold frame.4. The backlight unit of claim 3 , wherein a thickness of the support member increases when a distance from the first sidewall of the mold frame increases.5. The backlight unit of claim 4 , further comprising a joining member disposed between the support member and the inclined surface of the mold frame.6. The backlight unit of claim 3 , wherein the upper surface of the support member and the inclined surface of the support member are spaced apart from each other claim 3 , providing an optical path in an empty space ...

Multi-display device

A multi-display device includes a plurality of display modules and a host controller. Each of the display modules includes a display panel and a display driving integrated circuit and performs a display panel self-refresh operation. The host controller controls the display modules and provides image data for displaying an image to the display modules. The display modules are classified into a master display module and slave display modules that adjust a vertical blank period based on a reference tearing effect control signal output from the master display module. Thus, the multi-display device can mitigate or prevent a tearing effect from occurring between the display modules without changing an interface between the host controller and the display modules.

PRIMERLESS BASE PAINTING COMPOSITION FOR PARTS OF VEHICLE, KIT COMPRISING THE SAME, PAINTED PARTS OF VEHICLE COMPRISING THE SAME AND METHOD FOR PAINTING AND VARNISHING PARTS OF VEHICLE USING THE SAME

The present invention relates to a primerless base painting composition for parts of a vehicle comprising a resin component including a non-chlorinated modified polyolefin resin and an acrylic resin; a pigment; additives; and a solvent selected from an aqueous solvent and an oil solvent, a kit comprising the same, painted parts of a vehicle comprising the same, and a method for painting and varnishing parts of a vehicle using the same. 1. A primerless base painting composition for parts of a vehicle , comprising:a resin component including a non-chlorinated modified polyolefin resin and an acrylic resin;a pigment; additives; anda solvent selected from an aqueous solvent and an oil solvent.2. The primerless base painting composition for parts of a vehicle of claim 1 , wherein the non-chlorinated modified polyolefin resin is modified to at least one of maleic acid and maleic anhydride.3. The primerless base painting composition for parts of a vehicle of claim 1 , wherein the non-chlorinated modified polyolefin resin is modified to at least one of a hydroxyl group and a carboxylic acid group.4. The primerless base painting composition for parts of a vehicle of claim 1 , wherein the resin gradient further includes a water dispersible polyurethane resin.5. The primerless base painting composition for parts of a vehicle of claim 1 , wherein the composition includes:30 to 50 wt % of non-chlorinated modified polyolefin;1 to 10 wt % of acrylic resin;10 to 30 wt % of water dispersible polyurethane resin;5 to 20 wt % of pigment;0.1 to 10 wt % of additives; and10 to 40 wt % of aqueous solvent.6. The primerless base painting composition for parts of a vehicle of claim 1 , wherein the composition includes:10 to 30 wt % of non-chlorinated modified polyolefin;10 to 30 wt % of acrylic resin;5 to 20 wt % of pigment;0.1 to 10 wt % of additives; and10 to 40 wt % of oil solvent.7. A kit for painting and varnishing parts of a vehicle comprising a first gradient including the primerless ...

METHODS AND COMPOSITIONS FOR T-RNA BASED GUIDE RNA EXPRESSION

Compositions and methods are provided for editing nucleotides and/or altering target sites in the genome of a cell. The methods and compositions employ a recombinant DNA construct comprising a tRNA promoter operably linked to a polynucleotide encoding a single guide RNA, wherein said recombinant DNA construct does not comprise a nucleotide sequence encoding a ribozyme, wherein said guide RNA is capable of forming a guide RNA/Cas endonuclease complex, wherein said complex can bind to and cleave a target site sequence in the genome of a cell such as a microbial cell. The present disclosure further describes methods and compositions employing a recombinant DNA construct comprising a tRNA promoter operably linked to a spacer sequence and a polynucleotide encoding a single guide RNA, wherein said recombinant DNA construct does not comprise a nucleotide sequence encoding a ribozyme, wherein said guide RNA is capable of forming a guide RNA/Cas endonuclease complex, wherein said complex can bind to and cleave a target site sequence in the genome of a non-conventional yeast. 1. A recombinant DNA construct comprising a tRNA promoter operably linked to a polynucleotide encoding a single guide RNA , wherein said recombinant DNA construct does not comprise a nucleotide sequence encoding a ribozyme , wherein said guide RNA is capable of forming a guide RNA/Cas endonuclease complex , wherein said complex can bind to and cleave a target site sequence in the genome of a non-conventional yeast.2. The recombinant DNA of claim 1 , wherein the tRNA promoter is selected from the group consisting of a tRNA or a tRNA fragment capable of functioning as a promoter sequence.3. The recombinant DNA of claim 2 , wherein the tRNA is selected from the group consisting of tRNA-Lys claim 2 , tRNA-Val claim 2 , tRNA-Glu claim 2 , tRNA Leu claim 2 , tRNA-ile claim 2 , tRNA-trp claim 2 , tRNA-tyr claim 2 , tRNA-his claim 2 , and any one combination thereof.4. The recombinant DNA of claim 2 , wherein ...

METHOD AND APPARATUS FOR DIRECT CONVERSION RECEIVER CORRECTING DIRECT CURRENT OFFSET

The present invention relates to a method and an apparatus for direct current offset calibration of a direct conversion receiver, a Direct Current (DC) offset calibration apparatus of a direct conversion receiver includes a plurality of variable gain amplifiers for amplifying an input signal based on a gain control value, a DC offset monitoring unit for monitoring a DC offset for an output signal of the plurality of variable gain amplifiers, a plurality of variable Digital to Analog Converters (DACs) for controlling a current applied to each of the plurality of variable gain amplifiers according to a current control code, and a DC offset cancellation unit for determining a current control code set which minimizes the DC offset value per preset gain control value, and thus the DC offset can be precisely cancelled without being affected by external factors such as a signal modulation method and heat and performance degradation of the receiver can be prevented. 1. An apparatus for Direct Current (DC) offset calibration of a direct conversion receiver , the apparatus comprising:a plurality of variable gain amplifiers for amplifying an input signal based on a gain control value;a DC offset monitoring unit for monitoring a DC offset for an output signal of the plurality of variable gain amplifiers;a plurality of variable Digital to Analog Converters (DACs) for controlling a current applied to each of the plurality of variable gain amplifiers according to a current control code; anda DC offset cancellation unit for determining a current control code set which minimizes the DC offset value per preset gain control value.2. The apparatus of claim 1 , wherein the variable DAC comprises:a plurality of switches for being turned on/off according to the current control code;a plurality of current sources connected to the plurality of switches respectively and generating a current corresponding to a power of two; anda base current control circuit for providing a base current to the ...

BACKLIGHT UNIT AND DISPLAY DEVICE HAVING THE SAME

A backlight unit and a display device including the same are provided. The backlight unit includes a light source; a mold frame configured to accommodate the light source and having a window frame shape with an opening therein; and a supporting member disposed on the mold frame and comprising an optical pattern, wherein the mold frame comprises a first sidewall, and an inclined surface protruding obliquely downward and inward from an inner side surface of the first sidewall, and wherein the supporting member is disposed on the inclined surface of the mold frame. 1. A backlight unit comprising:a light source;a mold frame configured to accommodate the light source and having a window frame shape with an opening therein; anda support on the mold frame and comprising an optical pattern,wherein the mold frame comprises a first sidewall, and an inclined surface protruding obliquely downward and inward from an inner side surface of the first sidewall, andwherein the support is on the inclined surface of the mold frame.2. The backlight unit of claim 1 , wherein the support comprises an upper surface oriented vertically to the first sidewall of the mold frame claim 1 , and an inclined surface extended obliquely downward and inward at a same inclination angle as the inclined surface of the mold frame.3. The backlight unit of claim 2 , wherein the optical pattern is formed in the upper surface and/or the inclined surface of the support.4. The backlight unit of claim 3 , wherein the optical pattern is formed by patterning at least one of the surfaces of the support.5. The backlight unit of claim 3 , wherein the optical pattern has a prism or a lenticular shape.6. The backlight unit of claim 5 , wherein the optical pattern is extended in a same direction as a direction in which the support is extended.7. The backlight unit of claim 3 , wherein a thickness of the support increases in a direction away from the first sidewall of the mold frame.8. The backlight unit of claim 3 , ...

COLOR COATING COMPOSITION FOR LED LAMP DIFFUSER USING GLASS FRITS AND COLOR-COATED GLASS ARTICLE USING THE SAME

Disclosed herein are a color coating composition for an LED lamp diffuser using glass frits and a color-coated glass article using the same. The color coating composition is capable of increasing durability and a life of an LED lamp, satisfactorily maintaining an external appearance and a lighting quality thereof for a long time, and realizing various colors, by manufacturing a glass-made diffuser as a means for diffusing light of the LED lamp in a manner of coating the diffuser on various sheets of transparent or translucent glass such as tubes and bulbs so that the diffuser is not deformed and discolored due to light and heat, and has high strength and translucency. 1. A color coating composition for an LED lamp diffuser , comprising:a binder solution, glass fits, ceramic filler, and an inorganic pigment in a specified composition.2. The color coating composition according to claim 1 , wherein the color coating composition comprises 50 wt % of a binder solution claim 1 , 40 wt % to 44 wt % of glass fits claim 1 , 5 wt % to 8 wt % of ceramic filler claim 1 , and 1 wt % to 3 wt % of an inorganic pigment.3. The color coating composition according to claim 2 , wherein the inorganic pigment comprises any one selected from the group including C.I. Pigment Red 108 claim 2 , Cadmium zinc sulfide claim 2 , C.I. Pigment Greed 50 claim 2 , and C.I. Pigment Blue 28.4. The color coating composition according to claim 3 , wherein the inorganic pigment has a content of 1 wt % to 5 wt %.5. The color coating composition according to claim 2 , wherein the binder solution is composed of an organic binder and a solvent.6. The color coating composition according to claim 5 , wherein the organic binder comprises any one selected from the group including nitrocellulose claim 5 , acrylic resin claim 5 , and ethyl cellulose claim 5 , and wherein the solvent comprises one or more components selected from the group including toluene claim 5 , acetone claim 5 , isobutylacetate claim 5 , ...

COLOR COATING COMPOSITION FOR LED LAMP DIFFUSER AND COLOR-COATED GLASS ARTICLE USING THE SAME

Disclosed herein are a color coating composition for an LED lamp diffuser and a color-coated glass article using the same. The color coating composition is capable of increasing durability and a life of an LED lamp, satisfactorily maintaining an external appearance and a lighting quality thereof for a long time, and realizing various colors, by manufacturing a glass-made diffuser as a means for diffusing light of the LED lamp in a manner of coating the diffuser on various sheets of transparent or translucent glass such as tubes and bulbs so that the diffuser is not deformed and discolored due to light and heat, and has high strength and translucency. 1. A color coating composition for an LED lamp diffuser , comprising:80 wt % of a binder solution; 15 wt % to 19 wt % of ceramic filler; and 1 wt % to 5 wt % of an inorganic pigment.2. The color coating composition according to claim 1 , wherein the binder solution contains 10 wt % to 30 wt % of a solvent component in modified urethane silicone resin.3. The color coating composition according to claim 1 , wherein claim 1 , the solvent component comprises any one selected from the group including toluene claim 1 , acetone isobutylacetate claim 1 , butyle cellosolve claim 1 , and xylene.4. The color coating composition according to claim 1 , wherein the ceramic filler comprises one or more components selected from the group including calcium carbonate claim 1 , calcium oxide claim 1 , calcium fluoride claim 1 , silica dioxide claim 1 , diatomite claim 1 , magnesium oxide claim 1 , aluminum oxide claim 1 , and zinc oxide.5. The color coating composition according to claim 1 , wherein the inorganic pigment comprises any one selected from the group including C.I. Pigment Red 108 claim 1 , Cadmium zinc sulfide claim 1 , C.I. Pigment Greed 50 claim 1 , and C.I. Pigment Blue 28.6. A color-coated glass article coated with a color coating composition for an LED lamp diffuser claim 1 , wherein the color-coated glass article is ...

HIGHLY ELASTIC AQUEOUS ADHESIVE COMPOSITION AND METHOD OF SURFACE-TREATING MOLDED ARTICLE USING THE SAME

Disclosed are a adhesive composition and a method of surface-treating a molded article using the same. In particular, the adhesive composition may be eco-friendly because smell and VOC generation from the organic solvent are reduced by adding a water-dilutable polyurethane resin having a hydroxyl group to a waterborne polyurethane resin, thereby improving properties such as elastic recoverability and scratch resistance while satisfying conventional properties. 1. An adhesive composition comprising:an amount of about 10 to 30% by weight of a water-dilutable polyurethane resin having a hydroxyl group;an amount of about 20 to 40% by weight of a waterborne polyurethane resin;an amount of about 5 to 20% by weight of a hardener;an amount of about 3 to 10% by weight of an extender pigment;an amount of about 0.1 to 5.0% by weight of a wetting agent;an amount of about 0.1 to 3.0% by weight of a slip agent;an amount of about 0.1 to 2.0% by weight of an antifoaming agent;an amount of about 0.01 to 2.00% by weight of a hardening accelerator;an amount of about 0.5 to 5.0% by weight of a light stabilizer;an amount of about 0.1 to 3.0% by weight of a thickener; andan amount of about 20 to 40% by weight of a solvent.all the % by weight based on the total weight of the adhesive composition.2. The adhesive composition according to claim 1 , wherein the water-dilutable polyurethane resin having a hydroxyl group comprises: a) isocyanate having two or more functional groups on average; b) hydrophilic polyol having two or more functional groups on average; c) polyester polyol; d) a solvent; and e) a neutralizing agent.3. The adhesive composition according to claim 2 , wherein the water-dilutable polyurethane resin having a hydroxyl group comprises: a) an amount of about 1 to 10% by weight of the isocyanate having two or more functional groups on average; b) an amount of about 1 to 10% by weight of the hydrophilic polyol having two or more functional groups on average; c) an amount of ...

High stereospecific polybutylene polymer and highly active process for preparation thereof

Disclosed is a process for the preparation of a high stereospecific (isotactic) polybutylene polymer comprising the step of polymerizing a reactive monomer, 1-butene, which is used or is not used as a solvent, in the presence of catalyst and inert gas. According to the present invention, it is possible to prepare a high stereospecific polybutylene polymer in much higher activity than that of any other known processes for the preparation of a high stereospecific polybutylene polymer. The high stereospecific polybutylene polymer according to the present invention is a homopolymer of 1-butene, or a copolymer containing a-olefin and up to 40% by weight of a comonomer, wherein titanium in the catalyst residues is not detected in the ppm level, stereospecificity (Isotactic Index, mmmm %) determined by 13 C-NMR is 96 or more, molecular weight distribution (Mw/Mn) is 3-6, and molecular weight distribution (Mw/Mn) can be controlled to 8 or more.

High stereospecific polybutylene polymer and highly active process for preparation thereof

Disclosed is a process for the preparation of a high stereospecific(isotactic) polybutylene polymer comprising the step of polymerizing a reactive monomer, 1­-butene, which is used or is not used as a solvent, in the presence of catalyst and inert gas. According to the present invention, it is possible to prepare a high stereospecific polybutylene polymer in much higher activity than that of any other known processes for the preparation of a high stereospecific polybutylene polymer. The high stereospecific polybutylene polymer according to the present invention is a homopolymer of 1-butene, or a copolymer containing a-olefin and up to 40% by weight of a comonomer, wherein titanium in the catalyst residues is not detected in the ppm level, stereospecificity(Isotactic Index, mmmm%) determined by 13C-NMR is 96 or more, molecular weight distribution(Mw/Mn) is 3-6, and molecular weight distribution(Mw/Mn) can be controlled to 8 or more.

High stereospecific polybutylene polymer and highly active process for preparation thereof

Disclosed is a process for the preparation of a high stereospecific(isotactic) polybutylene polymer comprising the step of polymerizing a reactive monomer, 1­-butene, which is used or is not used as a solvent, in the presence of catalyst and inert gas. According to the present invention, it is possible to prepare a high stereospecific polybutylene polymer in much higher activity than that of any other known processes for the preparation of a high stereospecific polybutylene polymer. The high stereospecific polybutylene polymer according to the present invention is a homopolymer of 1-butene, or a copolymer containing a-olefin and up to 40% by weight of a comonomer, wherein titanium in the catalyst residues is not detected in the ppm level, stereospecificity(Isotactic Index, mmmm%) determined by 13C-NMR is 96 or more, molecular weight distribution(Mw/Mn) is 3-6, and molecular weight distribution(Mw/Mn) can be controlled to 8 or more.

High stereospecific polybutylene polymer and highly active process for preparation thereof

High stereospecific polybutylene polymer and highly active process for preparation thereof

Disclosed is a process for the preparation of a high stereospecific(isotactic) polybutylene polymer comprising the step of polymerizing a reactive monomer, 1­-butene, which is used or is not used as a solvent, in the presence of catalyst and inert gas. According to the present invention, it is possible to prepare a high stereospecific polybutylene polymer in much higher activity than that of any other known processes for the preparation of a high stereospecific polybutylene polymer. The high stereospecific polybutylene polymer according to the present invention is a homopolymer of 1-butene, or a copolymer containing a-olefin and up to 40% by weight of a comonomer, wherein titanium in the catalyst residues is not detected in the ppm level, stereospecificity(Isotactic Index, mmmm%) determined by 13C-NMR is 96 or more, molecular weight distribution(Mw/Mn) is 3-6, and molecular weight distribution(Mw/Mn) can be controlled to 8 or more.

Macromolecular weight poly(gamma-glutamic acid) and its use

PURPOSE: A macromolecular weight poly(γ-glutamic acid) and hydrated gel, cosmetics, food, feed, humectant and Ca absorbing auxiliary containing the poly(γ-glutamic acid) are provided, to obtain a material having excellent hygroscopicity, moisturizing effect, Ca dissolving effect and absorption. CONSTITUTION: The macromolecular weight poly(γ-glutamic acid) has an average molecular weight of 5,000,000 Da or more. Preferably the macromolecular weight poly(γ -glutamic acid) has an average molecular weight of 10,000,000 Da or more. Preferably the macromolecular weight poly(γ-glutamic acid) is produced from Bacillus subtilis var chungkukjang (KCTC 0697BP).

Mutant hpgg motif and hdash motif delta-5 desaturases and their use in making polyunsaturated fatty acids

Mutant delta 5 desaturases, having the ability to convert dihomo-gamma-linolenic acid [DGLA; 20:3 omega-6] to arachidonic acid [ARA; 20:4 omega-6] and/or eicosatetraenoic acid [ETA; 20:4 omega-3] to eicosapentaenoic acid [EPA; 20:5 omega-3] and possessing at least one mutation within the HPGG (SEQ ID NO:7) motif of the cytochome b 5 -like domain and at least one mutation within the HDASH (SEQ ID NO:8) motif are disclosed. Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding delta 5 desaturases, along with a method of making long chain polyunsaturated fatty acids ["PUFAs"], are also disclosed.

Expression of caleosin in recombinant oleaginous microorganisms to increase oil content therein

Recombinant oleaginous microorganisms having increased oil content due to the expression of a caleosin polypeptide are described. A recombinant oleaginous microorganism of the disclosed invention produces at least 25% of its dry cell weight as oil, and comprises a functional polyunsaturated fatty acid (PUFA) biosynthetic pathway and at least one genetic construct encoding a caleosin polypeptide. A method for increasing the amount of oil in a recombinant oleaginous microorganism is also described.

Method and device for extending beam area in wireless communication system

An electronic device is provided in a wireless communication system. The device comprises a plurality of antenna sets; a plurality of antenna elements configuring the plurality of antenna sets; an RF transceiver including a plurality of switches for selecting the plurality of antenna elements and a plurality of phase shifters for shifting the phase of a signal transmitted/received through the plurality of antenna elements; and a control unit for determining a beam forming direction and the phase of the signal by simultaneously controlling the plurality of switches and the plurality of phase shifters according to a beambook.

Methods and compositions for polymerase ii (pol-ii) based guide rna expression

Compositions and methods are provided for editing nucleotides and/or altering target sites in the genome of a cell. The methods and compositions employ a recombinant DNA construct comprising a Pol-ll promoter operably linked to a polynucleotide encoding a single guide RNA, wherein said guide RNA is capable of forming a guide RNA/Cas endonuclease complex, wherein said complex can bind to and cleave a target site sequence in the genome of a cell such as a eukaryote cell. The present disclosure further describes methods and compositions employing said recombinant DNA construct to modify the genome of non-conventional yeast.

Peroxisome Biogenesis Factor Protein (PEX) Breakage to Change the Contents of Polyunsaturated Fatty Acids and Total Lipid Content in Oily Eukaryotic Organisms

transformed yarrowia lipolytica and method for producing at least one non-native product of interest

Increased ethanol production by yeast harboring constitutive transcriptional activator mal alleles

Described are compositions and methods relating to modified yeast harboring constitutive transcriptional activator MAL alleles. The yeast produces an increased amount of ethanol, or have an increased rate of ethanol production, which does not appear to be the result of maltose metabolism. Such yeast is particularly useful for ethanol production utilizing starch substrates.

Recombinant microbial cells that produce at least 28% eicosapentaenoic acid as dry cell weight

Recombinant microbial cells are disclosed herein that produce an oil comprising at least 28 percent eicosapentaenoic acid (EPA) measured as a weight percent of dry cell weight. These cells may comprise a polynucleotide sequence encoding an active acyl-CoA:lysophosphatidylcholine acyltransferase (LPCAT) comprising at least one amino acid mutation in a membrane-bound O-acyltransferase motif. In addition, the cells may comprise a down-regulation of an endogenous polynucleotide sequence encoding Sou2 sorbitol utilization protein, and/or one or more polynucleotides encoding phospholipid:diacylglycerol acyltransferase (PDAT), delta-12 desaturase, a dihomo-gamma-linolenic acid (DGLA) synthase multizyme, delta-8 desaturase, malonyl-CoA synthetase (MCS), or acyl-CoA:lysophosphatidic acid acyltransferase (LPAAT). Also disclosed are methods of using the recombinant microbial cells to produce oil containing omega-3 polyunsaturated fatty acids such as EPA.

Expression of caleosin in recombinant oleaginous microorganisms to increase oil content therein

Recombinant oleaginous microorganisms having increased oil content due to the expression of a caleosin polypeptide are described. A recombinant oleaginous microorganism of the disclosed invention produces at least 25% of its dry cell weight as oil, and comprises a functional polyunsaturated fatty acid (PUFA) biosynthetic pathway and at least one genetic construct encoding a caleosin polypeptide. A method for increasing the amount of oil in a recombinant oleaginous microorganism is also described.

Method for outputting screen according to force input and electronic device supporting the same

Recombinant microbial host cells for high eicosapentaenoic acid production

Improved yeasts for brewing

The present invention relates to flavor stabilized beers and to methods of making flavor stabilized beers. More specifically, the instant disclosure provides methods and compositions for the production of flavor stabilized beer having low riboflavin using modified yeast to ferment a wort.

Cell surface expression vector of sars virus antigen and microorganisms transformed thereby

The present invention relates to a surface expression vector of SARS coronavirus antigen containing a gene encoding an antigen of SARS inducing coronavirus and any one or two or more of genes pgsB, pgsC and pgsA encoding poly-gamma-glutamic acid synthase complex, a microorganism transformed by the surface expression vector, and a SARS vaccine comprising the microorganism. According to the present invention, it is possible to economically produce a vaccine for prevention and treatment of SARS using a recombinant strain expressing an SARS coronavirus antigen on their surface.

High eicosapentaenoic acid oils from improved optimized strains of Yarrowia lipolytica

Described are engineered strains of the oleaginous yeast capable of producing an oil comprising greater than 50 weight percent of eicosapentaenoic acid ["EPA"], an ω-3 polyunsaturated fatty acid, measured as a weight percent of total fatty acids ["% TFAs"] and having a ratio of at least 3.1 of EPA % TFAs, to linoleic acid, measured as % TFAs. These strains over-express at least one Δ9 elongase/Δ8 desaturase multizyme, in addition to other heterologous Δ9 elongases, Δ8 desaturases, Δ5 desaturases, Δ17 desaturases, Δ12 desaturases, C elongases, and over-express malonyl CoA synthetases and/or acyl-CoA lysophospholipid acyltransferases. The strains possess at least one peroxisome biogenesis factor protein knockout. Methods for producing EPA within said host cells, oils obtained from the cells, and products therefrom are claimed.

Recombinant microbial host cells for high eicosapentaenoic acid production

Engineered strains of the oleaginous yeast Yarrowia lipolytica are disclosed herein that are capable of producing microbial oil comprising greater than 25 weight percent of eicosapentaenoic acid ["EPA"], an omega-3 polyunsaturated fatty acid, measured as a weight percent of dry cell weight.

Cell surface expression vector of SARS virus antigen and microorganisms transformed thereby

SURFACE EXPRESSION VECTORS WITH pgsBCA, THE GENE GENERATING POLY GAMMA GLUTAMATE SYNTHETASE, AND METHOD FOR EXPRESSING A TARGET PROTEIN ON THE SURFACE OF A MICROORGANISM USING THE VECTOR

Peptide-mediated delivery of rna-guided endonuclease into cells

A composition is disclosed that comprises at least one protein component of an RNA-guided endonuclease (RGEN) and at least one cell-penetrating peptide (CPP), wherein the RGEN protein component and CPP are covalently or non-covalently linked to each other in an RGEN protein-CPP complex. The RGEN protein-CPP complex can traverse (i) a cell membrane, or (ii) a cell wall and cell membrane, of a cell. The RGEN protein component of an RGEN protein-CPP complex in certain embodiments can be associated with a suitable RNA component to provide an RGEN capable of specific DNA targeting. Further disclosed are compositions comprising at least one protein component of a guide polynucleotide/Cas endonuclease complex and at least one CPP, as well as methods of delivering RGEN proteins into microbial cells, as well as methods of targeting DNA with RGENs.

Peptide-mediated delivery of rna-guided endonuclease into cells

A composition is disclosed that comprises at least one protein component of an RNA-guided endonuclease (RGEN) and at least one cell-penetrating peptide (CPP), wherein the RGEN protein component and CPP are covalently or non-covalently linked to each other in an RGEN protein-CPP complex. The RGEN protein-CPP complex can traverse (i) a cell membrane, or (ii) a cell wall and cell membrane, of a cell. The RGEN protein component of an RGEN protein-CPP complex in certain embodiments can be associated with a suitable RNA component to provide an RGEN capable of specific DNA targeting. Further disclosed are compositions comprising at least one protein component of a guide polynucleotide/Cas endonuclease complex and at least one CPP, as well as methods of delivering RGEN proteins into microbial cells, as well as methods of targeting DNA with RGENs.

Composition for adjuvant containing poly-gamma-glutamic acid

High eicosapentaenoic acid oils from improved optimized strains of yarrowia lipolytica

Described are engineered strains of the oleaginous yeast Yarrowia lipolytica capable of producing an oil comprising greater than 50 weight percent of eicosapentaenoic acid ["EPA"], an ?-3 polyunsaturated fatty acid, measured as a weight percent of total fatty acids ["% TFAs"] and having a ratio of at least 3.1 of EPA % TFAs, to linoleic acid, measured as % TFAs. These strains over-express at least one ?9 elongase/?8 desaturase multizyme, in addition to other heterologous ?9 elongases, ?8 desaturases, ?5 desaturases, ?17 desaturases, ?12 desaturases, C16/18 elongases, and over-express malonyl CoA synthetases and/or acyl-CoA lysophospholipid acyltransferases. The strains possess at least one peroxisome biogenesis factor protein knockout. Methods for producing EPA within said host cells, oils obtained from the cells, and products therefrom are claimed.

High eicosapentaenoic acid oils from improved optimized strains of yarrowia lipolytica

Described are engineered strains of the oleaginous yeast Yarrowia lipolytica capable of producing an oil comprising greater than 50 weight percent of eicosapentaenoic acid ["EPA"], an ω-3 polyunsaturated fatty acid, measured as a weight percent of total fatty acids ["% TFAs"] and having a ratio of at least 3.1 of EPA % TFAs, to linoleic acid, measured as % TFAs. These strains over-express at least one Δ9 elongase/Δ8 desaturase multizyme, in addition to other heterologous Δ9 elongases, Δ8 desaturases, Δ5 desaturases, Δ17 desaturases, Δ12 desaturases, C 16/18 elongases, and over-express malonyl CoA synthetases and/or acyl-CoA lysophospholipid acyltransferases. The strains possess at least one peroxisome biogenesis factor protein knockout. Methods for producing EPA within said host cells, oils obtained from the cells, and products therefrom are claimed.

Peptide-mediated delivery of RNA-guided endonuclease into cells

A composition is disclosed that comprises at least one protein component of an RNA-guided endonuclease (RGEN) and at least one cell-penetrating peptide (CPP), wherein the RGEN protein component and CPP are covalently or non-covalently linked to each other in an RGEN protein-CPP complex. The RGEN protein-CPP complex can traverse (i) a cell membrane, or (ii) a cell wall and cell membrane, of a cell. The RGEN protein component of an RGEN protein-CPP complex in certain embodiments can be associated with a suitable RNA component to provide an RGEN capable of specific DNA targeting. Further disclosed are compositions comprising at least one protein component of a guide polynucleotide/Cas endonuclease complex and at least one CPP, as well as methods of delivering RGEN proteins into microbial cells, as well as methods of targeting DNA with RGENs.

High eicosapentaenoic acid oils from improved optimized strains of yarrowia lipolytica

Poly-gamma-glutamate having ultra high molecular weight and method for using the same

The present invention relates to a poly-gamma-glutamate (PGA) having an ultra-high molecular weight greater than 5,000 kDa. The ultra-high molecular weight PGA according to the present invention has a mean molecular weight greater than 13,000 kDa, and more than 95 % of its molecules have a molecular weight ranging from 3,000 to 15,000 kDa. Also, it can be produced by the culturing of Bacillus subtilis var. chungkookjang. The ultra-high molecular weight PGA according to the present invention shows very excellent moisture-absorbing, moisture-retaining, sustained release, mineral solubility, and water-absorbing properties, and thus, can be used as a new and high value-added material in various applications.

Genetic targeting in non-conventional yeast using an rna-guided endonuclease

Non-conventional yeasts are disclosed herein comprising at least one RNA-guided endonuclease (RGEN) comprising at least one RNA component that does not have a 5'-cap. This uncapped RNA component comprises a sequence complementary to a target site sequence in a chromosome or episome in the yeast. The RGEN can bind to, and optionally cleave, one or both DNA strands at the target site sequence. An example of an RGEN herein is a complex of a Cas9 protein with a guide RNA. A ribozyme is used in certain embodiments to provide an RNA component lacking a 5'-cap. Further disclosed are methods of genetic targeting in non-conventional yeast.

Improved optimized strains of yarrowia lipolytica for high eicosapentaenoic acid production

Described are engineered strains of the oleaginous yeast Yarrowia lipolytica capable of producing an oil comprising greater than 50 weight percent of eicosapentaenoic acid ["EPA"], an ω-3 polyunsaturated fatty acid, measured as a weight percent of total fatty acids ["% TFAs"] and having a ratio of at least 3.1 of EPA % TFAs, to linoleic acid, measured as % TFAs. These strains over-express at least one Δ9 elongase/Δ8 desaturase multizyme, in addition to other heterologous Δ9 elongases, Δ8 desaturases, Δ5 desaturases, Δ17 desaturases, Δ12 desaturases, C 16/18 elongases, and optionally over-express diacylglycerol cholinephosphotransferases, malonyl CoA synthetases and/or acyl-CoA lysophospholipid acyltransferases. The expression of at least one peroxisome biogenesis factor protein is down-regulated. Methods for producing EPA within said host cells, oils obtained from the cells, and products therefrom are claimed.

Aldolase promoter for L. casei

Improved optimized strains of Yarrowia lipolytica for high eicosapentaenoic acid production

Described are engineered strains of the oleaginous yeast capable of producing an oil comprising greater than 50 weight percent of eicosapentaenoic acid ["EPA"], an ω-3 polyunsaturated fatty acid, measured as a weight percent of total fatty acids ["% TFAs"] and having a ratio of at least 3.1 of EPA % TFAs, to linoleic acid, measured as % TFAs. These strains over-express at least one Δ9 elongase/Δ8 desaturase multizyme, in addition to other heterologous Δ9 elongases, Δ8 desaturases, Δ5 desaturases, Δ17 desaturases, Δ12 desaturases, C elongases, and optionally over-express diacylglycerol cholinephosphotransferases, malonyl CoA synthetases and/or acyl-CoA lysophospholipid acyltransferases. The expression of at least one peroxisome biogenesis factor protein is down-regulated. Methods for producing EPA within said host cells, oils obtained from the cells, and products therefrom are claimed.

A novel obligately symbiotic thermophile symbiobacterium toebii sc-1 producing thermostable l-tyrosine phenol-lyase and l-tryptophan indole-lyase and a method for screening its relative bacteria

The present invention relates to a novel obligately symbiotic thermophile Symbiobacterium toebii SC-1(Accession NO: KCTC 0685BP), and a thermostable L-tyrosine phenol-lyase and L-tryptophan indole-lyase produced by Symbiobacterium toebii SC-1. The present invention also relates to a pure culture method of Symbiobacterium toebii SC-1, a method of growth measurement for Symbiobacterium toebii SC-1 and its relative symbiotic bacteria showing low-growth yield using nitrate respiration, and a screening method of relative symbiotic strains of Symbiobacterium toebii SC-1 from the environment using a specific antibody to it. Therefore, the thermostable L-tyrosine phenol-lyase and L-tryptophan indole-lyase produced by Symbiobacterium toebii SC-1 in this invention can provide more stable catalysts in enzymatic biotransformation processes which enzymatically produce valuable medico-amino acids 3,4-dehydroxy-L-phenylalanine (L-DOPA) and L-tryptophan.

HIGH-EICOSAPENTAIC ACID OILS FROM IMPROVED AND OPTIMIZED STRAINS OF YARROWIA LIPOLYTICA

High stereospecific polybutylene polymer and highly active process for preparation thereof

Manipulation of snf1 protein kinase activity for altered oil content in oleaginous organisms

Methods of increasing the total lipid content in a eukaryotic cell, the total content of polyunsaturated fatty acids [PUFAs], and/or the ratio of desaturated fatty acids to saturated fatty acids by reducing the activity of the heterotrimeric SNF1 protein kinase are disclosed. Preferably, the chromosomal genes encoding the Snf1 x-subunit, Gal83 .beta.-subunit or Snf4 .gamma.-subunit of the SNF1 protein kinase, the upstream regulatory genes encoding Sak1, Hxk2, Glk1 or Reg1, or the downstream genes encoding Rme1, Cbr1 or Snf3 are manipulated in a PUFA-producing strain of the oleaginous yeast Yarrowia lipolytica, resulting in increased total lipid content, as compared to the parent strain comprising the heterotrimeric SNF1 protein kinase not having reduced activity.

SURFACE EXPRESSION VECTORS HAVING pgsBCA, THE GENE CODING POLY-GAMMA-GLUTAMATE SYNTHETASE, AND A METHOD FOR EXPRESSION OF TARGET PROTEIN AT THE SURFACE OF MICROORGANISM USING THE VECTOR

The present invention relates to a surface expression vector having pgsBCA, a gene coding poly-gamma-glutamate synthetase and a method for expression of target protein at the surface of microorganism using the vector. The vector, in which foreign genes are inserted, transforms microorganisms and makes foreign proteins expressed stably on the surface of microorganisms.

Improved optimized strains of Yarrowia lipolytica for high eicosapentaenoic acid production

Described are engineered strains of the oleaginous yeast

Recombinant microbial cells that produce at least 28% aicosapentaenoic acid as dry cell weight

Poly-gamma-glutamate having ultra high molecular weight and method for using the same

VEHICLE LIGHT WITH ROTATING LIGHT SOURCE

Eine Fahrzeugleuchte mit einer sich drehenden Lichtquelle kann einen Signalempfänger, der ein Signal von einem oder mehreren Sensoren, die in einem Fahrzeug vorgesehen sind, empfängt; einen Leuchtdioden-(LED)-Abschnitt, der eine oder mehrere LED-Elemente aufweist, die konfiguriert sind, Licht in Richtung einer Außenseite des Fahrzeugs hin zu emitteren; einen Controller, der mit dem einen oder den mehreren LED-Elementen verbunden ist und konfiguriert ist, eine Lichterzeugungsmenge des einen oder der mehreren LED-Elemente zu steuern; einen Signalsender, der mit dem Signalempfänger verbunden ist, das Signal von dem Signalempfänger empfängt und das empfangene Signal an den Controller sendet; und ein Antriebselement, der mit dem LED-Abschnitt gekoppelt ist und konfiguriert ist, den LED-Abschnitt zu drehen, wobei der Controller die Lichterzeugungsmenge des einen oder der mehreren LED-Elemente als eine Reaktion auf das Signal steuert, enthalten. A vehicle lamp with a rotating light source may have a signal receiver that receives a signal from one or more sensors provided in a vehicle; a light emitting diode (LED) section having one or more LED elements configured to emit light toward an outside of the vehicle; a controller connected to the one or more LED elements and configured to control a light generation amount of the one or more LED elements; a signal transmitter connected to the signal receiver, receiving the signal from the signal receiver, and sending the received signal to the controller; and a drive element coupled to the LED section and configured to rotate the LED section, the controller controlling the amount of light generation of the one or more LED elements in response to the signal.

Highly elastic aqueous adhesive composition and method of surface-treating molded article using the same

【課題】既存の物性をそのまま満足しながらも弾性回復力及び耐スクラッチ性を向上させることができる高弾性水性接着剤組成物、及び製造コストを節減するとともに柔らかい触感のスエード質感を具現することができる自動車内装材用プラスチック成形品の表面処理方法を提供する。 【解決手段】水酸基を持つ水希釈性ポリウレタン樹脂１０〜３０重量％、水分散性ポリウレタン樹脂２０〜４０重量％、硬化剤５〜２０重量％、体質顔料３〜１０重量％、湿潤剤０．１〜５．０重量％、スリップ剤０．１〜３．０重量％、消泡剤０．１〜２．０重量％、硬化促進剤０．０１〜２．００重量％、光安定剤０．５〜５．０重量％、増粘剤０．１〜３．０重量％、及び溶剤２０〜４０重量％を含むことを特徴とする。 【選択図】なし

Vector for anti-HPV vaccine and transformed microorganism by the vector

Expression vectors that can efficiently produce virion capsid protein, tumor-associated protein of human papillomavirus on a microbial surface. Bacterial strains harboring such surface display vectors, and the use of the bacterial strains or their extracts or purified products as complex vaccines, are also described. The surface display vectors contain one or more than two genes selected from among pgsB, pgsC and pgsA, encoding a poly-χ-glutamic acid synthetase complex (pgsBCA) of a Bacillus sp. strain, and genes that encode virion capsid proteins, tumor-associated proteins of human papillomavirus. Methods for preparing the foregoing vectors, vaccines and transformed microorganisms are also described.

Composição de cuidados com a pele, uso, produto de cuidados com a pele e métodos para tratar um distúrbio de couro cabeludo e para tratar e/ou reduzir uma afecção de caspa do couro cabeludo

COMPOSIÇÃO DE CUIDADOS COM A PELE, USO, PRODUTO DE CUIDADOS COM A PELE E MÉTODOS PARA TRATAR UM DISTÚRBIO DE COURO CABELUDO E PARA TRATAR E/OU REDUZIR UMA AFECÇÃO DE CASPA DO COURO CABELUDO. A presente revelação é direcionada a composições de cuidados com a pele, formulações de cuidados com a pele e métodos para fornecer tratamento de distúrbios de couro cabeludo. Mais especificamente, a presente revelação é direcionada a métodos e composições que compreendem pelo menos um microrganismo do gênero, e/ou uma fração do mesmo e/ou um lisato celular do mesmo e/ou fermentado do mesmo e/ou metabólito do mesmo para tratar um distúrbio de couro cabeludo, incluindo caspa.

Methods and compositions for microbial treatment of skin disorders

The present disclosure is directed towards skin care compositions, skin care formulations, and methods for providing treatment of scalp disorders. More specifically, the present disclosure is directed towards methods and compositions comprising a Bacillus velezensis fermentate, and/or metabolite thereof, for treating a scalp disorder, including dandruff.

Oberflächenexpressionsvektoren mit pgsbca, dem für poly-gamma-glutamat-synthetase codierenden gen, sowie verfahren zur expression eines zielproteins an der oberfläche eines den vektor benutzenden mikroorganismus

Hochstereospezifisches polybutylenpolymer und hochaktives verfahren zu dessen herstellung