Поиск патентов

05-01-2012 дата публикации

Process and Reactor System for Depolymerization of Polymeric Biomass

Номер: US20120003724A1

Автор: Banibrata Pandey, Deepti Agrawal, K Ramya, Kumar Manoj Sarkar, Prabhat Goel, Soumya Sasmal

Принадлежит: Nagarjuna Energy Pvt Ltd

The present invention provides a continuous process for saccharification of cellulose by enzymatic degration without any loss of enzymes and also discloses a bioreactor for performing said process.

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Номер записи: 1

12-01-2012 дата публикации

Biomass hydrothermal decomposition system and saccharide-solution production method using biomass material

Номер: US20120009642A1

Автор: Hideo Suzuki, Yoshio Kuromi, Yoshitaka Kimura

Принадлежит: Mitsubishi Heavy Industries Ltd

A biomass hydrothermal decomposition system includes a hydrothermal decomposition unit 17 that transports the fed biomass material from a lower side to an upper side in an apparatus body 13 by screw means 14, feeds pressurized hot water 15 from an upper side different from a feed position of the biomass material 11 into the apparatus body 13, which is pressurized hot water to be discharged, so as to separate a lignin component and a hemicellulose component from the biomass material; a biomass solid discharging unit 18 that discharges a biomass solid 20 from the upper side of the apparatus body 13; and a slurrying vessel 21 communicating with the biomass solid discharging unit 18, into which water 19 is injected and the discharged biomass solid 20 is added to obtain a slurried biomass solid are provided.

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Номер записи: 2

12-01-2012 дата публикации

Biotechnological Production of Chondroitin

Номер: US20120010399A1

Автор: Antonio Trilli, Francesca Bagatin, Immacolata Busiello, Simona Daly

Принадлежит: GNOSIS SPA

Chondroitin is produced by culturing a recombinant microorganism which is obtained by inactivation of a gene encoding an enzyme responsible for addition of fructose residues to the linear chondroitin polysaccharide in a microorganism producing a fructosylated derivative of chondroitin.

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Номер записи: 3

16-02-2012 дата публикации

Cellobiose 2-epimerase, its preparation and uses

Номер: US20120040407A1

Автор: Hikaru Watanabe, Hiroto Chaen, Masahiro Yagi, Shigeharu Fukuda, Tomoyuki Nishimoto

Принадлежит: Hayashibara Seibutsu Kagaku Kenkyujo KK

The present invention has objects to provide a thermostable cellobiose 2-epimerase, its preparation and uses. The present invention attains the above objects by providing a thermostable cellobiose 2-epimerase, a DNA encoding the enzyme, a recombinant DNA and transformant comprising the DNA, a process for producing the enzyme, and a process for producing isomerized saccharides using the enzyme.

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Номер записи: 4

23-02-2012 дата публикации

Method for purifying glucose polymers for peritoneal dialysis solutions

Номер: US20120046460A1

Автор: Marc Biguet, Pierrick Duflot, Stephane Bourdain

Принадлежит: Roquette Freres SA

The invention relates to a method of purifying glucose polymers for the production of peritoneal dialysis solutions, characterized in that it includes at least one step of processing activated carbon and/or granular black, at least one sterilizing filtration step, at least one heat treatment step, and at least one ultrafiltration step.

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Номер записи: 5

08-03-2012 дата публикации

Methods for increasing the yield of fermentable sugars from plant stover

Номер: US20120058524A1

Автор: Michael R. Ladisch, Nathan S. Mosier, Willem Evert Vermerris

Принадлежит: PURDUE RESEARCH FOUNDATION

Methods for increasing yield of fermentable sugars from plant stover are provided. The methods include using plants homozygous for two brown midrib mutations, bm1 and bm3. The methods also include using plants homozygous for a mutation in a gene that results in reduced cinnamyl alcohol dehydrogenase activity, and a mutation in a gene that results in reduced 5-hydroxyconiferaldehyde/5-hydroxyconiferyl alcohol O-methyltransferase activity. The methods also include using transgenic plants that have reduced cinnamyl alcohol dehydrogenase activity and reduced 5-hydroxyconiferaldehyde/5-hydroxyconiferyl alcohol O-methyltransferase activity in comparison with wild-type plants.

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Номер записи: 6

24-05-2012 дата публикации

Continuously fed biomass pretreatment process for a packed bed reactor

Номер: US20120125548A1

Автор: Jeffrey David Cohen

Принадлежит: EI Du Pont de Nemours and Co

Biomass pretreatment using anhydrous ammonia is effective in a static reactor vessel when the ammonia can penetrate through the biomass particles or pieces in vapor state, and when biomass is continuously fed and moved through the reactor. To achieve this condition, total system moisture content is kept below 40 weight % based on total mass in the system. The pretreated biomass product is effectively saccharified to produce fermentable sugars for biocatalyst production of a product.

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Номер записи: 7

31-05-2012 дата публикации

Hmo synthesis

Номер: US20120135467A1

Автор: Eric Huefner, Julia Parkot, Stefan Jennewein

Принадлежит: JENNEWEIN BIOTECHNOLOGIE GMBH

The present invention relates to a cell to be stably cultured in a medium, which cell is adjusted for the production of oligosaccharides, the cell being transformed to comprise at least one nucleic acid sequence coding for an enzyme involved in oligosaccharide synthesis. In addition the cell is transformed to comprise at least one nucleic acid sequence coding for a protein of the sugar efflux transporter family, a functional homolog or derivative thereof. Further, the invention concerns a method for the production of oligosaccharides involving above cell.

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Номер записи: 8

07-06-2012 дата публикации

Insoluble dietary fiber-containing materials derived from cereal seeds

Номер: US20120141455A1

Автор: Hideo Ohmae, Hiroshi Murayama, Hiroyuki Watanabe, Mikio Katayama, Naohiro Takimoto, Osamu Kanauchi, Yuji Sakamoto, Yuta Komano

Принадлежит: Kirin Holdings Co Ltd

This invention provides a product safe for treating, improving or preventing an inflammatory bowel disease such as ulcerative colitis, and provides a product comprising an insoluble dietary fiber obtained by enzymatic treatment of seeds of a grain plant(s) or germinated young seeds thereof, as well as a food or drink or medicament comprising the product.

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Номер записи: 9

07-06-2012 дата публикации

Viscosity control in compositions comprising plant fiber materials

Номер: US20120142909A1

Автор: Brock Lundberg

Принадлежит: Fiberstar Bio Ingredient Technologies Inc

Pectinases, such as Pectinex™ Ultra SP-L (composed of the enzyme Polygatacturonase, a type of pectinase which is derived from Aspergillus aculeatus ) or pectinmethylesterases were used to decrease or increase, respectively, the viscosity of fiber solutions, especially solutions with highly refined cellulosic thickeners, and particularly those made of highly refined cellulosic parenchyma cell wall fiber solutions. The enzyme can reduce the viscosity up to 95% or increase the viscosity 100 fold. At lower concentrations the enzyme requires up to a few days of reacting to reach the full reduction in viscosity. Pectinex™ Ultra SP-L has an optimum pH of 4.5-5 and a temperature optimum of 40° C. By controlling the viscosity available from the dried, treated highly refined cellulosic fiber compositions, tailored powder compositions can be provided that will provide precise viscosities when rehydrated in solutions at a constant concentration.

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Номер записи: 10

28-06-2012 дата публикации

Recombinant beta-glucosidase variants for production of soluble sugars from cellulosic biomass

Номер: US20120164696A1

Автор: Attila Andor, Jie Yang, Xiyun Zhang

Принадлежит: Codexis Inc

The invention relates to recombinant expression of a variant form of a fungal C1 strain β-glucosidase. The invention also relates to the generation of fermentable sugars from biomass and the production of biofuels by fermentation of the sugars using genetically modified organisms expressing the β-glucosidase variant. The invention provides methods for producing a fermentable sugar, such as glucose, from cellobiose by contacting cellobiose with a recombinant β-glucosidase variant protein, such as a variant protein secreted by a recombinant host cell into culture medium. Methods of the invention may be used for conversion of a biomass substrate to a fermentable sugar, and ultimately to ethanol or other biofuel.

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Номер записи: 11

26-07-2012 дата публикации

Culture Medium and Methods for Producing Alginate From Stable Mucoid Strains of Pseudomonas Aeruginosa

Номер: US20120190077A1

Автор: Hongwei David Yu, Kristy Dawn Dillon, Richard M. NILES, XIN Wang

Принадлежит: PROGENESIS Tech LLC

A specialized culture medium for the promotion of alginate production by stable mucoid Pseudomonas aeruginosa bacterial strains and methods for the production and purification of industrial, commercial, and pharmaceutical grade alginate from bacteriological sources are provided herein. Alginate produced using the media and methods disclosed herein is structurally uniform and substantially free of bacterial cell contaminants, including endotoxin.

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Номер записи: 12

18-10-2012 дата публикации

Sugar mixtures and methods for production and use thereof

Номер: US20120264873A1

Автор: Aharon Meir Eyal, Asher Vitner, Revital Mali, Robert P. Jansen

Принадлежит: Individual

A sugar mixture comprising: monosaccharides; oligosaccharides in a ratio ≧0.06 to total saccharides; disaccharides in a ratio to total saccharides ≧0.05; pentose in a ratio to total saccharides ≧0.05; at least one alpha-bonded di-glucose; and at least one beta-bonded di-glucose. Also disclosed are methods to make and/or use such mixtures.

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Номер записи: 13

25-10-2012 дата публикации

Variant humicola grisea cbh1.1

Номер: US20120270270A1

Автор: Colin Mitchinson, Edmund Larenas, Frits Goedegebuur, Peter Gualfetti

Принадлежит: DANISCO US INC

Disclosed are variants of Humicola grisea Cel7A (CBH1.1), H. jecorina CBH1 variant or S. thermophilium CBH1, nucleic acids encoding the same and methods for producing the same. The variant cellulases have the amino acid sequence of a glycosyl hydrolase of family 7A wherein one or more amino acid residues are substituted.

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Номер записи: 14

01-11-2012 дата публикации

Compositions and methods comprising cellulase variants with reduced affinity to non-cellulosic materials

Номер: US20120276595A1

Автор: Amy D. Liu, Bradley R. Kelemen, Luis G. Cascao-Pereira, Thijs Kaper

Принадлежит: DANISCO US INC

The present disclosure relates to cellulase variants. In particular the present disclosure relates to cellulase variants having reduced binding to non-cellulosic materials. Also described are nucleic acids encoding the cellulase, compositions comprising said cellulase, methods of identifying cellulose variants and methods of using the compositions.

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Номер записи: 15

01-11-2012 дата публикации

Process for production of fructo-oligosaccharides

Номер: US20120276597A1

Автор: Ganapathi Patil, Maheswaran Palamalai, Manoj Gote, Saravanan Rengarajan, Uday Kashinath Avalakki

Принадлежит: Tata Chemicals Ltd

A microbial consortium comprises of an Aureobasidium sp. to metabolise a sugar substrate into fructooligosaccaride, glucose and fructose and a Pachysolen sp to metabolise the glucose and the fructose into ethanol.

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Номер записи: 16

08-11-2012 дата публикации

Enhanced soluble c5 saccharide yields

Номер: US20120282655A1

Автор: Phillip R. Gibbs

Принадлежит: Renmatix Inc

Methods are disclosed for increasing the level of soluble C 5 saccharides produced from lignocellulosic biomass comprising acidifying fractionated lignocellulosic biomass to prevent the recondensation of soluble C 5 saccharides, including C 5 oligosaccharides and xylose and arabinose monomers, to insoluble higher molecular weight C 5 oligosaccharides.

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Номер записи: 17

13-12-2012 дата публикации

Saccharide-solution producing apparatus, fermentation system, saccharide-solution producing method, and fermentation method

Номер: US20120315677A1

Автор: Gaku Kondo, Hideo Suzuki, Michio Nishiyama, Minoru Genta, Seiichi Terakura

Принадлежит: Mitsubishi Heavy Industries Mechatronics Systems Ltd

A saccharide-solution producing apparatus 11 A according to the present invention is a saccharide-solution producing apparatus for producing a saccharide solution 22 derived from a carbohydrate-based material 21 , and includes a saccharide-solution controlling unit 15 A that controls the saccharide solution derived from the carbohydrate-based material 21 , a cellulosic biomass saccharifying unit 16 that saccharifies hydrothermally treated biomass obtained by hydrothermally decomposing a cellulosic biomass material 35 that contains a lignin component and a hemicellulose component, and produces a diluted saccharide solution 37 , and a diluted-saccharide-solution supply pipe L 11 that mixes the diluted saccharide solution 37 produced by the cellulosic biomass saccharifying unit 16 into the saccharide-solution controlling unit 15 A. With this configuration, it is possible to improve production efficiency of the saccharide solution 22 and to realize cost reduction.

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Номер записи: 18

03-01-2013 дата публикации

Method for producing saccharified solution

Номер: US20130004997A1

Автор: Shigenobu Mitsuzawa

Принадлежит: Honda Motor Co Ltd

A method for producing a saccharified solution is provided, by which a saccharide recovered as a saccharified solution can be increased. The saccharified solution is obtained by treating a substrate solution containing lignocellulosic biomass as a substrate with a saccharifying enzyme produced by a microorganism to prepare a substrate/saccharifying enzyme mixture liquid, and removing a residue of the substrate from the substrate/saccharifying enzyme mixture liquid. The concentration of the substrate in the substrate/saccharifying enzyme mixture liquid is adjusted to be in the range of 15 to 30% by mass. In the removal of the residue of the substrate, a saccharide adsorbed on the residue is extracted.

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Номер записи: 19

10-01-2013 дата публикации

Food product containing starch gel, starch granule, production method and use thereof

Номер: US20130011884A1

Автор: Junya FUKUDA, Kenichi Kurita, Masakazu Kimura, Takashi Ichihara

Принадлежит: Glico Foods Co Ltd

Here is provided a method of producing a starch gel-containing food, the method comprising the steps of: treating starch granules with an enzyme at a temperature of about 10° C. or higher and about 70° C. or lower to obtain an enzyme-treated starch; mixing a food material, the enzyme-treated starch and water to obtain a mixture; heating the mixture thereby gelatinizing the enzyme-treated starch in the mixture; and cooling the mixture containing the gelatinized enzyme-treated starch thereby gelling the starch to obtain a starch gel-containing food, wherein the enzyme is selected from the group consisting of amyloglucosidase, isoamylase, α-glucosidase, α-amylase having a characteristic capable of improving a gel forming ability of a starch, and cyclodextrin glucanotransferase.

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Номер записи: 20

24-01-2013 дата публикации

Bacterial extract for digestive or urinary tract disorders and process for its preparation

Номер: US20130022695A1

Автор: Carlo Chiavaroli, Jacques Alain Bauer, Jean-Pierre Leon Vigroux, Laetitia Chalvet, Marco Salvagni

Принадлежит: OM PHARMA SA

The present invention relates to an extract from bacterial strains useful as a treatment for disorders such as digestive or urinary tract disorders, compositions comprising the extract, and processes of making the extract from media that do not pose a risk of prion diseases.

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Номер записи: 21

24-01-2013 дата публикации

Cooling and processing materials

Номер: US20130023020A1

Автор: Marshall Medoff

Принадлежит: Xyleco Inc

Systems and methods for cooling and processing materials are disclosed.

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Номер записи: 22

21-02-2013 дата публикации

Novel Beta-Glucosidase and Uses thereof

Номер: US20130045510A1

Автор: Chen-Wei Li, Hsion-Wen Kuo, Ng I-Son Wu, Su-May Yu, Tuan-Hua David Ho, Yu-Ming Ju

Принадлежит: Academia Sinica

A novel beta-glucosidase and nucleic acids encoding the beta-glucosidase. Also disclosed are cells, compositions, and methods relating to using the beta-glucosidase to convert ligocellulosic material to fermentable sugars.

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Номер записи: 23

28-02-2013 дата публикации

Fine fibrous cellulosic material and process for producing the same

Номер: US20130052695A1

Автор: Manami Sakai, Naomi Kadotani, Noriko Tanaka, Seung-Hwan Lee, Takashi Endo, Yoshikuni Teramoto

Принадлежит: National Institute of Advanced Industrial Science and Technology AIST

To provide a fine fibrous cellulosic material capable of producing a saccharide in a high yield by hydrolysis; to provide a process for producing the fine fibrous cellulosic material from a cellulosic material; and to provide a process for producing the saccharide using the fine fibrous cellulosic material. The present invention is the fine fibrous cellulosic material containing cellulose, hemicellulose and lignin, which the fine fibrous cellulosic material has a width of 1 μm or less and a length of 5,000 μm or less and is used for glycation reaction by hydrolysis.

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Номер записи: 24

14-03-2013 дата публикации

System for the treatment of biomass

Номер: US20130065289A1

Автор: David Charles Carlson

Принадлежит: Poet Research Inc

A system for treating biomass for the production of ethanol is disclosed. A biorefinery for producing a fermentation product from biomass is disclosed. The biorefinery comprises a system for preparing the biomass into prepared biomass and a system for pre-treating the biomass into pre-treated biomass. The biorefinery comprises a separator, a first treatment system, a second treatment system, and a fermentation system. A method for producing a fermentation product from biomass is disclosed.

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Номер записи: 25

28-03-2013 дата публикации

Pretreatment Method for Producing Water-Soluble Sugars From Lignocellulosic Material

Номер: US20130078695A1

Автор: Jaakko Palola, Janne Sandqvist, Paivi Rousu

Принадлежит: CHEMPOLIS OY

The invention relates to manufacturing hydrolyzable cellulose and further, if desired, sugars from lignocellulosic material by means of formic and performic acid treatment.

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Номер записи: 26

04-04-2013 дата публикации

Method of Producing a Diutan Gum

Номер: US20130084607A1

Автор: Coleman Russell, Harding Nancy E., Matzke Steven, Patel Yamini N.

Принадлежит: CP KELCO U.S., INC.

The production of a diutan polysaccharide exhibiting increased viscosity properties as compared with previously produced polysaccharide of the same type of repeating units. Such an improved diutan polysaccharide is produced through the generation of a derivative of sp. ATCC 53159 that harbors a multicopy broad host range plasmid into which genes for biosynthesis of diutan polysaccharide have been cloned. The inventive methods of production of such an improved diutan polysaccharide, as well as the novel cloned genes required to produce the improved diutan within such a method, are also encompassed within this invention. Additionally, the novel engineered strain including the needed DNA sequence is encompassed within this invention. 116-. (canceled)17. A method of producing a diutan gum comprising:{'i': 'Sphingomonas', 'introducing a coding sequence for at least one diutan biosynthetic enzyme into a host diutan producing organism;'}culturing the host organism under fermentation conditions, whereby the host organism produces a diutan gum which exhibits at least one of the following characteristics:a) an intrinsic viscosity of greater than 150 dL/g;b) a sea water 3 rpm viscosity greater than 35 dial reading;c) a sea water 0.3 rpm viscosity greater than 35,000 centipoise; andd) a low shear rate viscosity in the presence of polyethylene glycol dispersant of greater than 3500 centipoise.18. The method of wherein the at least one diutan biosynthetic enzyme is a DpsG polymerase.19. The method of wherein the at least one diutan biosynthetic enzyme comprises a DpsG polymerase and a glucose-1-phosphate thymidylyltransferase; a dTDP-6-deoxy-D-glucose-3-5-epimerase; a dTDP-D-glucose-4 claim 17 ,6-dehydratase; and a dTDP-6-deoxy-L-mannose-dehydrogenase.20. The method of wherein the at least one diutan biosynthetic enzyme comprises a DpsG polymerase and a rhamnosyl transferase IV; a glucosyl-isoprenylphosphate transferase I; a beta-1 claim 17 ,4-glucuronosyl transferase II; and a ...

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Номер записи: 27

11-04-2013 дата публикации

METHOD OF PRODUCING A HYDROGEL

Номер: US20130089592A1

Автор: Chimphango Annie Fabian Abel, Gorgens Johann Ferdinand, Van Zyl Willem Heber

Принадлежит: STELLENBOSCH UNIVERSITY

The invention provides a method of enzymatically modifying xylan by selectively removing glucuronic acid and/or arabinose side chains from the xylan with a-D-glucuronidase and/or a-L-arabinofuranosidase, and allowing the modified xylan to form into a hydrogel when the xylan becomes insoluble. A bioactive substance, such as a protein, enzyme, antimicrobial agent, bactericide or pharmaceutical compound can be added to the xylan so that the substance is encapsulated within the hydrogel or incorporated onto its surface. The hydrogel can be used as a drug delivery agent, such as for sustained-release or targeted drug delivery, rectal drug delivery or a dressing for a wound, burn or scar. The hydrogel can also be used as a coating, such as on medical gloves, catheters, surgical drainage systems, utensils or the like, or can be used in a scaffold for tissue engineering. 1. A method of producing a hydrogel , the method comprising the steps of:enzymatically modifying xylan which contains glucuronic acid or a derivative thereof and/or arabinose side chains so that it has reduced solubility in water compared to naturally occurring xylan, by selectively removing glucuronic acid and/or arabinose side chains from the xylan with one or both of α-D-glucuronidase and α-L-arabinofuranosidase; andallowing the modified xylan to form a hydrogel which encapsulates a bioactive substance, wherein the bioactive substance is brought into contact with the modified xylan either before the hydrogel forms or after the hydrogel forms.2. A method according to claim 1 , which further includes the steps of contacting the modified xylan with a bioactive substance and allowing the bioactive substance to be immobilized on or within the hydrogel.3. A method according to claim 2 , wherein the bioactive substance is added to the xylan before the hydrogel forms.4. A method according to claim 2 , wherein the bioactive substance is added to the xylan after formation of the hydrogel.5. A method according to ...

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Номер записи: 28

18-04-2013 дата публикации

POLYPEPTIDE HAVING ACETYL XYLAN ESTERASE ACTIVITY AND USES THEREOF

Номер: US20130095532A1

Автор: Heijne Wilbert Herman Marie, Los Alrik Pieter, Schoonneveld-Bergmans Margot Elisabeth Francoise

Принадлежит: DSM IP ASSETS B.V.

The invention relates to a polypeptide comprising the amino acid sequence set out in SEQ ID NO: 2 or an amino acid sequence encoded by the nucleotide sequence of SEQ ID NO: 1, or a variant polypeptide or variant polynucleotide thereof, wherein the variant polypeptide has at least 82% sequence identity with the sequence set out in SEQ ID NO: 2 or the variant polynucleotide encodes a polypeptide that has at least 82% sequence identity with the sequence set out in SEQ ID NO: 2. The invention features the full length coding sequence of the novel gene as well as the amino acid sequence of the full-length functional polypeptide and functional equivalents of the gene or the amino acid sequence. The invention also relates to methods for using the polypeptide in industrial processes. Also included in the invention are cells transformed with a polynucleotide according to the invention suitable for producing these proteins. 1. A polypeptide which comprises the amino acid sequence set out in SEQ ID NO: 2 or an amino acid sequence encoded by the nucleotide sequence of SEQ ID NO:1 , or a variant polypeptide or variant polynucleotide thereof , wherein the variant polypeptide has at least 82% sequence identity with the sequence set out in SEQ ID NO: 2 or the variant polynucleotide encodes a polypeptide that has at least 82% sequence identity with the sequence set out in SEQ ID NO: 2.2. The polypeptide according to claim 1 , wherein said polypeptide has acetyl xylan esterase activity.3. A polynucleotide which comprises:(a) a nucleotide sequence set out in SEQ ID NO: 1; or(b) a nucleotide sequence which hybridizes selectively with a polynucleotide being a reverse complement of SEQ ID NO: 1; or(c) a nucleotide sequence having at least 80% sequence identity with the nucleotide sequence of SEQ ID NO: 1; or(d) a fragment which is at least about 100 nucleotides in length of a nucleotide sequence as defined in (a), (b) or (c) which is at least about 100 nucleotides in length; or(e) a ...

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Номер записи: 29

09-05-2013 дата публикации

Low-viscosity reduced-sugar syrup, methods of making, and applications thereof

Номер: US20130112192A1

Автор: Guo-Hua Zheng, Lawrence E. Fosdick, Scott Helstad, Yauching W. Jasinski

Принадлежит: Cargill Inc

The invention provides a low-viscosity reduced-sugar syrup, methods of making such a low-viscosity reduced-sugar syrup, and uses of such syrup.

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Номер записи: 30

09-05-2013 дата публикации

Method of producing sugar

Номер: US20130115660A1

Автор: John Aikens, Robert J. Turner

Принадлежит: PROTERRO Inc

Provided herein is a transgenic bacteria engineered to accumulate carbohydrates, for example disaccharides. Also provided is a photobioreactor for cultivating photosynthetic microorganisms comprising a non-gelatinous, solid cultivation support suitable for providing nutrients and moisture to photosynthetic microorganisms and a physical barrier covering at least a portion of the surface of the cultivation support. Devices for the large scale and continuous cultivation of photosynthetic microorganisms incorporating photobioreactors and methods of use are disclosed. Also disclosed are methods of producing fermentable sugar from photosynthetic microorganisms using a photobioreactor of the invention.

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Номер записи: 31

23-05-2013 дата публикации

Method of treating plant biomass

Номер: US20130130328A1

Автор: Haruo Takahashi, Kazuhide Tabata, Nobuhiro Ishida, Risa Nakamura, Satoshi Katahira

Принадлежит: Toyota Motor Corp

Plant biomass is immersed in a solution that contains a polar solvent and an imidazolium salt that has a melting point of at least 100° C. As a result, the cellulose and hemicellulose present in the plant biomass are relaxed (decrystallized and depolymerized) and brought into an easy-to-degrade state. Reacting the immersed plant biomass with a cellulase produces saccharide at a high conversion efficiency.

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Номер записи: 32

23-05-2013 дата публикации

METHOD FOR EXTRACTING SUBSTANCES FROM SOAPBERRY FRUIT AND ITS SEEDS

Номер: US20130130329A1

Автор: Hsu Heng-Jui

Принадлежит:

An exclusive method for extracting active interface saponin and organic substances from soapberry; organic elements and oleic alcohol products from soapberry seeds through fermenting process, and end products made therefrom. By this method, every part of the soapberry is processed to become the raw material of varies of products and daily necessaries. Said method is toxin free and biologically safe, produce no solid and liquid wastage, zero carbon emissions, zero chemical pollution, low energy consumption and ecologically friendly. The end products produced by present invention are variables which can be applied in cosmetics, medicals, cleaning products, skin caring products and so on, thus, are with excellent economic value and industrial viability in mass production. 1. A method for extracting substances from soapberry fruit and its seeds , comprises following steps:{'b': 1', '1, 'Step : separate raw Soapberry fruits into pericarps and seeds, and store said pericarps under a constant temperature (T) for a predetermined time (S) to make them fermented naturally;'}{'b': 2', '1', '1', '1', '1, 'Step : put the fermented pericarps in step one inside a sealed tank, than fill in said sealed tank with liquid (W), then stand for 5-10 minutes, to make the foreign object attached on said pericarps being dissolved or moved by said liquid (W), supersonic vibrator or shifting the air pressure inside said sealed tank could be applied for speeding up the detachment of the foreign object on said pericarps, finally, take the pericarps out of the tank, and the liquid left in the tank becomes product which contains enzyme produced in step by fermenting;'}{'b': '3', 'Step : Put cleaned soapberry pericarps into a blender to grind them into the shattered mix of the pulp and fruit fiber;'}{'b': 4', '2', '2', '2, 'Step : Soak said shattered mix of the pulp and fruit fiber with liquid (W), then pouring them into an extractor; activate the extractor, and said pulp will be drained out of the ...

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Номер записи: 33

23-05-2013 дата публикации

Compositions Comprising High Molecular Weight Hyaluronic Acid and Methods For Producing Same

Номер: US20130131009A1

Автор: Andrei Seluanov, Christopher Hine, Vera Gorbunova

Принадлежит: UNIVERSITY OF ROCHESTER

This invention provides cell culture compositions which produce significant quantities of high molecular weight hyaluronic acid. The cell cultures are obtained from cells of mole rats, such as naked mole rats and blind mole rats. The high molecular weight hyaluronic acid can be collected in the conditioned media of these cell cultures. These cell cultures provide a convenient source of large quantities of high molecular weight hyaluronic acid.

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Номер записи: 34

30-05-2013 дата публикации

HIGH PURITY GENTIOOLIGOSACCHARIDES OBTAINED THEREFROM AND USES THEREOF

Номер: US20130136840A1

Автор: Ahn Sang Wook, Kim Kyeong Hee, LEE Jae Ho, Park Sang Jae

Принадлежит: CORN PRODUCTS DEVELOPMENT, INC.

The present invention relates to methods for preparing high purity gentiooligosaccharides, high purity gentiooligosaccharides obtained therefrom, and uses thereof. The methods of the present invention involve: adding a low purity gentiooligosaccharide to a liquid medium; subjecting the liquid medium to inoculation with a microorganism, followed by incubation and fermentation to consume glucose that is contained in the low purity gentiooligosaccharide; and subjecting the resulting fermentation broth to filtration and purification. According to the method of the present invention, high purity gentiooligosaccharides having a purity of at least 90% that can be used as alternatives to foods such as cocoa, chocolate, coffee, beer, tea, bread or confectionery product, and beverage or the main ingredients thereof. 1. A method for preparing a high purity gentiooligosaccharide comprising:adding a low purity gentiooligosaccharide to a liquid medium;subjecting the liquid medium to inoculation with a microorganism, followed by incubation and fermentation to consume glucose that is contained in the low purity gentiooligosaccharide;removing the microorganism from the obtained fermentation broth; andsubjecting the obtained broth to purification.2. The method of claim 1 , wherein the low purity gentiooligosaccharide contains 15 to 65% by weight of glucose and 35 to 85% by weight of gentiooligosaccharide.3. The method of claim 1 , wherein the low purity gentiooligosaccharide is added to the liquid medium at a concentration of 5 to 70% (w/v).4. The method of claim 1 , wherein the microorganism consumes glucose as a carbon source while not consuming gentiooligosaccharide.5. The method of claim 4 , wherein the microorganism is yeast claim 4 , bacteria or mold.6. A method for preparing a high purity gentiooligosaccharide comprising:adding a low purity gentiooligosaccharide to a liquid medium;subjecting the liquid medium to inoculation with a microorganism, followed by incubation and ...

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Номер записи: 35

06-06-2013 дата публикации

ALGA IN WHICH PRODUCTION OF PHOTOSYNTHETIC PRODUCTS IS IMPROVED, AND USE FOR SAID ALGA

Номер: US20130143260A1

Автор: Nishikawa Masanobu, Ogawa Kenichi

Принадлежит: JAPAN SCIENCE AND TECHNOLOGY AGENCY

Provided are an alga in which productivity of a photosynthate is increased, and use of the alga. The alga of the present invention has an increased glutathione concentration in its chloroplast. A method of the present invention for producing biomass is a method for producing biomass with the use of the alga of the present invention or an alga produced by a method of the present invention for producing an alga. 1. An alga having an increased glutathione concentration in a chloroplast thereof as a result of transformation caused by introduction , into the alga , of an exogenous polynucleotide encoding a protein which is at least one protein selected from the group consisting of γ-glutamylcysteine synthetase , glutathione synthetase , ATP-sulfurylase , adenosine 5′-phosphosulfate reductase , sulfite reductase , cysteine synthetase , and serine acetyl transferase.2. The alga according to claim 1 , wherein an expression amount and/or an activity of a protein are increased in the chloroplast claim 1 , said protein being at least one protein selected from the group consisting of γ-glutamylcysteine synthetase claim 1 , glutathione synthetase claim 1 , ATP-sulfurylase claim 1 , adenosine 5′-phosphosulfate reductase claim 1 , sulfite reductase claim 1 , cysteine synthetase claim 1 , and serine acetyl transferase.3. (canceled)4. The alga according to claim 1 , wherein the polynucleotide encoding the γ-glutamylcysteine synthetase is selected from the group consisting of the following (a) to (d):(a) a polynucleotide encoding a polypeptide consisting of the amino-acid sequence represented by SEQ ID NO: 1;(b) a polynucleotide encoding a polypeptide which consists of an amino-acid sequence with deletion, substitution, or addition of one or several amino acids in the amino-acid sequence represented by SEQ ID NO: 1 and which has a γ-glutamylcysteine synthetase activity;(c) a polynucleotide consisting of the nucleotide sequence represented by SEQ ID NO: 2; and(d) a polynucleotide ...

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Номер записи: 36

06-06-2013 дата публикации

Methods for Improving the Efficiency of Simultaneous Saccharification and Fermentation Reactions

Номер: US20130143277A1

Автор: Diner Bruce A., Fagan Paul Joseph, Gutierrez Christina, Hitz William D., Huang Tom T., Mitchinson Colin

Принадлежит: DANISCO US INC.

The present disclosure is directed, in a first aspect, to the use of inverting beta-xylosidase enzymes to reduce byproduct formation and increase the yield of fermentation products, as well as, in a second aspect, to the use of retaining beta-xylosidase enzymes to improve production of alkyl-beta-xylopyranoside compounds, in a simultaneous saccharification and fermentation reactions. 1. A method for simultaneous saccharification and fermentation (SSF) comprising culturing a complete fermentation medium , said complete fermentation medium comprising at least one fermenting microorganism , at least one xylan-containing biomass , at least one cellulase , at least one hemicellulase , and at least one inverting β-xylosidase , for a period and under conditions suitable for producing a fermentation product.2. The method of claim 1 , wherein the complete fermentation medium comprises an effective amount of the inverting β-xylosidase such that the complete fermentation medium produces less short chain alkyl-β-xylopyranoside (“AXP”) than does a control fermentation medium lacking the inverting β-xylosidase.3. The method of claim 1 , wherein the complete fermentation medium comprises an effective amount of the inverting β-xylosidase such that the complete fermentation medium produces at least 40% less AXP than does a control fermentation medium lacking the inverting β-xylosidase.4. The method of claim 3 , wherein the complete fermentation medium comprises an effective amount of the inverting β-xylosidase such that the complete fermentation medium produces at least 50% less AXP than does a control fermentation medium lacking the inverting β-xylosidase.5. The method of claim 4 , wherein the complete fermentation medium comprises an effective amount of the inverting β-xylosidase such that the complete fermentation medium produces at least 60% less AXP than does a control fermentation medium lacking the inverting β-xylosidase.6. The method of claim 5 , wherein the complete ...

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Номер записи: 37

20-06-2013 дата публикации

Hydrolysis of mannose-1-phospho-6-mannose linkage to phospho-6-mannose

Номер: US20130158239A1

Автор: Han Karel Remaut, Kathleen Camilla Telesphore Alida Maria Piens, Nico Luc Marc Callewaert, Petra Sophie Tiels, Wouter Vervecken

Принадлежит: Oxyrane UK Ltd, Universiteit Gent, Vlaams Instituut voor Biotechnologie VIB, Vrije Universiteit Brussel VUB

Described herein are methods and genetically engineered cells useful for uncapping a mannose-6-phosphate residue on an oligosaccharide.

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Номер записи: 38

18-07-2013 дата публикации

Method and Apparatus for Producing Engineered Fuel from High Cellulose Feedstock

Номер: US20130183715A1

Автор: Paul T. Baskis

Принадлежит: Individual

An apparatus and method for producing methane gas, synthetic hydrocarbon gas, and fertilizer is provided. The apparatus includes a mix tank for mixing cellulosic material with a solvent into a slurry and a generator having an exhaust. The apparatus further includes a stir tank reactor for converting the slurry to a solution containing lignin-like carbon and liquid, and a separator for separating the lignin-like carbon and liquid. An anaerobic digester decomposes the received liquid received from the stir tank into methane and liquid components. A carbon dioxide scrubber scrubs the methane component of carbon dioxide. The method includes mixing cellulosic material with a solvent into a slurry, and converting the slurry to a solution containing lignin-like carbon and liquid. It also includes separating the lignin-like carbon and liquid and decomposing the liquid into methane and liquid components, and scrubbing the methane component of carbon dioxide.

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Номер записи: 39

25-07-2013 дата публикации

FIBROUS MATERIALS AND COMPOSITES

Номер: US20130189738A1

Автор: Medoff Marshall

Принадлежит:

Fibrous materials, compositions that include fibrous materials, and uses of the fibrous materials and compositions are disclosed. For example, the fibrous materials can be operated on by a microorganism to produce ethanol or a by-product, such as a protein or lignin. 127-. (canceled)28. A method comprising:mechanically treating a cellulosic or lignocellulosic material to provide a stressed material having a BET surface area of greater than about 0.25 square meters per gram and a porosity of greater than about 25 percent; andcontacting the stressed material with an enzyme.29. The method of claim 28 , where the mechanical treatment is grinding.30. The method of claim 29 , where the grinding is stone grinding or pin grinding.31. The method of claim 28 , where the mechanical treatment is milling.32. The method of claim 31 , where the milling is air attrition milling.33. The method of claim 28 , where the mechanical treatment is shearing claim 28 , cutting claim 28 , ripping or tearing.34. The method of claim 33 , where the material is sheared with a rotary knife cutter.35. The method of claim 28 , where the cellulosic or lignocellulosic material is selected from the group consisting of grasses claim 28 , rice hulls claim 28 , bagasse claim 28 , cotton claim 28 , jute claim 28 , hemp claim 28 , flax claim 28 , bamboo claim 28 , sisal claim 28 , abaca claim 28 , straw claim 28 , corn cobs claim 28 , coconut hair and mixtures thereof.36. The method of claim 28 , where the cellulosic or lignocellulosic material is dry when it is mechanically treated.37. The method of claim 28 , where the cellulosic or lignocellulosic material is hydrated when it is mechanically treated.38. The method of claim 28 , where the cellulosic or lignocellulosic material is wet when it is mechanically treated.39. The method of claim 38 , where the cellulosic or lignocellulosic material is wet with water when it is mechanically treated.40. The method of claim 38 , where the cellulosic or ...

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Номер записи: 40

25-07-2013 дата публикации

Sphingomonas Strains Producing Greatly Increased Yield of PHB-Deficient Sphingan (Diutan)

Номер: US20130189748A1

Автор: Harding Nancy E., Patel Yamini N., Talashek Todd A.

Принадлежит: CP KELCO U.S., INC.

PHB-deficient strains having improved sphingan yield are provided. Certain of the strains are diutan-producing strains that exhibit a dramatic improvement in productivity and yield due to a combination of certain genetic modifications that affect PHB and sphingan synthesis. Moreover, the sphingans produced from such strains have superior characteristics including improved filterability, clarity, and improved rheology-modifying characteristics. The sphingans provided are, thus, highly desirable in a variety of commercial and industrial uses, including personal care items, cement applications, and oilfield applications. 1Sphingomonas,SphingomonasSphingomonas. A mutant strain of a genus comprising: at least one genetic modification that substantially or entirely eliminates a production of polyhydroxybutyrate (PHB); at least one genetic modification that results in increased production of a sphingan , wherein said genetic modification resulting in increased production of a sphingan comprises a genetic modification that increases the expression of at least one gene involved in sphingan synthesis , wherein said at least one gene involved in sphingan synthesis is selected from the group consisting of the genes contained in the insert in plasmids pS8 (SEQ ID NO: 1) , pX6 (SEQ ID NO: 54) , the plasmid contained in strain ATCC PTA-10102 , the plasmid contained in strain ATCC PTA-10103 , and homologs thereof; whereby the mutant strain of the genus produces an increased production of a sphingan that is essentially free of PHB relative to a congenic strain containing the at least one genetic modification that substantially or entirely eliminates the production of PHB and lacking the at least one genetic modification that results in increased production of a sphingan.2Sphingomonas. The mutant strain of the genus of claim 1 , wherein the sphingan is selected from the group consisting of diutan claim 1 , S-7 claim 1 , gellan claim 1 , S-88 claim 1 , welan claim 1 , rhamsan claim 1 ...

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Номер записи: 41

01-08-2013 дата публикации

BIOPHARMACEUTICAL PROCESS APPARATUSES ASSEMBLED INTO A COLUMN

Номер: US20130196375A1

Автор: Strobbe Per

Принадлежит: STROBBE TECH A/S

A bio factory apparatus capable of Up-Stream production of biologic material products or biologics products in a liquid volume and processing of biologic or biologic material products Down-Stream, comprising at least one first Up-Stream producing capsule and at least one second Down-Stream processing capsule, said capsules capable of being stacked in any order and any number within a column. 1. A bioreactor apparatus having a top capsule and a bottom capsule , the apparatus comprising a cultivation capsule having a production device for producing a biologic product , and a second upstream capsule , wherein said capsules are stacked and locked in a column , with the proviso that the top capsule and bottom capsule are not pump capsules at the same time.2. The apparatus of wherein the production device is in the top capsule or bottom capsule.3. The apparatus of further comprising an external fluid conveyance device claim 1 , such as a pump means claim 1 , connected to the column.4. The apparatus of comprising liquid nutrient media and micro organisms.5. The apparatus of wherein the top capsule and bottom capsule are in operable and liquid contact with each other and capable of regulating liquid to and from said bioreactor apparatus.6. The apparatus of wherein the production device comprises a porous matrix or a matrix body for retaining micro organisms.7. The apparatus of wherein the second up stream capsule is selected from a manifold capsule claim 1 , cultivation capsule claim 1 , active fluid conveyance capsule claim 1 , passive fluid conveyance capsule claim 1 , fluid collecting container claim 1 , valve capsule claim 1 , column end capsule claim 1 , conditioning capsule claim 1 , size adaptor capsule claim 1 , power supply capsules claim 1 , data acquisition capsules claim 1 , control capsule and storage capsule.8. The apparatus of claim 1 , comprising a further up-stream function selected from cultivation claim 1 , separation claim 1 , filtration claim 1 , ...

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Номер записи: 42

01-08-2013 дата публикации

Processing biomass

Номер: US20130196386A1

Автор: Marshall Medoff

Принадлежит: Xyleco Inc

Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful products, such as fuels. For example, systems are described that can use feedstock materials, such as cellulosic and/or lignocellulosic materials, to produce ethanol and/or butanol, e.g., by fermentation.

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Номер записи: 43

01-08-2013 дата публикации

Biocementation of particulate material in suspension

Номер: US20130196419A1

Автор: Jeannette Marisol Vera Araya, Johanna Del Rosario Obreque Contreras, Pamela Chavez Crooker, Pamela Gutierrez Saldano

Принадлежит: Cultivos Hidrobiologicos y Biotecnologia Aguamarina SA

The present invention is directed to a composition and method to decrease the amount of particulate material in suspension, both in a liquid or in air, especially in industrial processes that generate suspended particulate material. In particular, the invention is directed to a composition and method to decrease the amount of particulate material in suspension in air or a liquid through agglomeration and subsequent biocementation, by application of an exopolysaccharide (EPS) source that can be direct or through inoculation with microorganisms that produce said EPS. This allows in a first step to settle the particulate material and subsequently the cementation of the material when there are calcium containing compounds in the particulate material that has been settled in the first step, by means of the inoculation of a second class of microorganisms that have ureolytic activity.

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Номер записи: 44

08-08-2013 дата публикации

Manufacturing method for sugar solution and device for same

Номер: US20130203117A1

Автор: Atsushi Minamino, Hiroyuki Kurihara, Katsushige Yamada, Yuki Yamamoto

Принадлежит: TORAY INDUSTRIES INC

A method produces a sugar liquid by repeating a sugar liquid production process including (1) to (3): (1) adding a filamentous fungus-derived cellulase to cellulose to perform primary hydrolysis; (2) adding a fresh filamentous fungus-derived cellulase to the hydrolysate in Step (1) to perform secondary hydrolysis; and (3) subjecting the hydrolysate in Step (2) to solid-liquid separation to obtain a sugar liquid, from which a recovered enzyme is obtained; wherein the recovered enzyme obtained in Step (3) is used for Step (1) of the next and later sugar liquid production processes.

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Номер записи: 45

08-08-2013 дата публикации

VARIANT CBH I POLYPEPTIDES

Номер: US20130203128A1

Автор: Healey Shaun, LUGINBUHL PETER, Lyon Chris S., Poland John, Stege Justin T., Varvak Alexander

Принадлежит: BP Corporation North America Inc,

In alternative embodiments, the invention provides polypeptides having a lignocellulolytic (lignocellulosic) activity, e.g., a ligninolytic and cellulolytic activity, including, e.g., a glycosyl hydrolase, a cellulase, an endoglucanase, a cellobiohydrolase (cbhl) (e.g., an exo-cellobiohydrolase, e.g., having an “exo” activity that can processively release cellobiose units β-1,4 glucose-glucose disaccharide), a beta-glucosidase, a xylanase, a mannanse, a xylosidase (e.g., a (β-xylosidase) and/or an arabinofuranosidase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In one embodiment, the invention provides thermostable and thermotolerant forms of polypeptides of the invention. The polypeptides and nucleic acids of the invention are used in a variety of pharmaceutical, agricultural and industrial contexts; for example, as enzymes for the bioconversion of a biomass, e.g., lignocellulosic residues, into fermentable sugars, where in one aspect these sugars are used as a chemical feedstock for the production of ethanol and fuels, e.g., biofuels, e.g., synthetic liquid or gas fuels, including ethanol, methanol and the like. 1. A polypeptide comprising the amino acid sequence of a variant cellobiohydrolase I (“CBH I”) catalytic domain , said variant CBH I catalytic domain having at least 90% sequence identity to a reference catalytic domain corresponding to amino acid positions 26-455 of SEQ ID NO:134 , and which comprises one or more amino acid substitutions that result in increased activity or improved thermotolerance as compared to the reference catalytic domain.2. The polypeptide of claim 1 , which has one or more substitutions that result in increased activity as compared to the reference catalytic domain.3. The polypeptide of claim 2 , which has one or more of the following substitutions or combinations of substitutions: (a) N222H; (b) N222E; (c) S217K; (d) L225Y; (e) L225V; (f) D87L; (g) ...

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Номер записи: 46

15-08-2013 дата публикации

Methods of Degrading or Hydrolyzing a Polysaccharide

Номер: US20130210086A1

Автор: Eijsink Vincent G. H., Forsberg Zarah, Horn Svein J., Sorliie Morten, Vaaje-Kolstad Gustav, Westereng Bjorge

Принадлежит: NOVOZYMES A/S

The invention provides a method of degrading or hydrolyzing a polysaccharide, preferably cellulose or chitin, comprising contacting said polysaccharide with one or more oxidohydrolytic enzymes, preferably a CBM33 family protein (preferably CBP21) or a GH61 family protein, wherein said degradation or hydrolysis is carried out in the presence of at least one reducing agent and at least one divalent metal ion. A method of producing an organic substance comprising said method is also provided. 1. A method of degrading or hydrolyzing a polysaccharide comprising contacting said polysaccharide with one or more oxidohydrolytic enzymes , wherein said degradation or hydrolysis is carried out in the presence of at least one reducing agent and at least one divalent metal ion.2. The method of claim 1 , wherein said polysaccharide is chitin or cellulose.3. The method of claim 1 , wherein said oxidohydrolytic enzyme is a CBM33 family protein (preferably CBP21) or a GH61 family protein.4. The method of claim 1 , wherein two or more oxidohydrolytic enzymes are used in said method.5. The method of claim 4 , wherein one of said oxidohydrolytic enzymes is a CBM33 family protein and another of said oxidohydrolytic enzymes is a GH61 family protein.6. The method of claim 1 , wherein said oxidohydrolytic enzyme is a polypeptide which comprises an amino acid sequence as set forth in any one of SEQ ID NOs: 1 to 16 or a sequence with at least 30% sequence identity thereto or a biologically active fragment thereof comprising at least 100 amino acids of said sequence.7. The method of claim 1 , wherein said polysaccharide is cellulose.8. The method of claim 7 , wherein said oxidohydrolytic enzyme is a GH61 protein claim 7 , E7 claim 7 , CelS2 claim 7 , Cfla0175 claim 7 , Cfla0172 claim 7 , Cfla0316 claim 7 , Cfla0490 claim 7 , CJA2191 claim 7 , CJA3139 or SCO1734.9. The method of claim 7 , wherein said oxidohydrolytic enzyme is a polypeptide which comprises an amino acid sequence as set forth in ...

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Номер записи: 47

15-08-2013 дата публикации

Polypeptides Having Cellobiohydrolase Activity and Polynucleotides Encoding Same

Номер: US20130210087A1

Автор: Lan Tang, Ye Liu

Принадлежит: Novozymes Inc

The present invention provides isolated polypeptides having cellobiohydrolase activity and isolated polynucleotides encoding the polypeptides. The invention also provides nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

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Номер записи: 48

15-08-2013 дата публикации

PROCESS FOR THE PRODUCTION OF SUGARS FROM LIGNOCELLULOSIC BIOMASS PRE-TREATED WITH A MIXTURE OF HYDRATED INORGANIC SALTS AND METALLIC SALTS

Номер: US20130210089A1

Автор: Morvan Didier, OLIVIER-BOURBIGOU Helene, Pellier Emmanuel, Vallee Christophe

Принадлежит: IFP ENERGIES NOUVELLES

The present invention concerns a process for the conversion of lignocellulosic biomass into sugars, comprising at least three steps. The first step is a step for cooking the lignocellulosic biomass in the presence of at least one hydrated inorganic salt mixed with at least one other metallic salt. The second step is a step for separating at least one solid fraction which has undergone the cooking step, and the third step is a step for enzymatic hydrolysis of said solid fraction to convert the polysaccharides into monosaccharides. The sugars obtained thereby can then be fermented into alcohols. 1. A process for the conversion of lignocellulosic biomass into monosaccharides , comprising at least: {'br': None, 'sub': n', '2, 'MX.n′HO'}, 'a) a step for cooking the biomass in the presence or in the absence of an organic solvent in a medium comprising at least one hydrated organic salt with formula (1) {'br': None, 'sub': m', '2, 'M′Y.m′HO'}, 'in which X is an anion and M is a metal selected from groups 1 and 2 of the periodic classification of the elements, n is a whole number equal to 1 or 2 and n′ is in the range 0.5 to 6; mixed with at least one other metallic salt, which may or may not be hydrated, with general formula (2)in which Y is an anion, which may be identical to or different from X, and M′ is a metal selected from groups 3 to 13 of the periodic classification of the elements, m is a whole number in the range 1 to 6 and m′ is in the range 0 to 6;b) a step for separating a solid fraction which has undergone step a);c) a step for enzymatic hydrolysis of said solid fraction.2. A process according to claim 1 , in which the medium in which the cooking step is carried out is constituted by one or more hydrated inorganic salts with formula (1) mixed with at least one other metallic salt claim 1 , which may or may not be hydrated claim 1 , with formula (2).3. A process according to claim 1 , in which the anion X is a halide anion selected from Cl claim 1 , F claim 1 ...

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Номер записи: 49

15-08-2013 дата публикации

Polypeptides Having Endoglucanase Activity And Polynucleotides Encoding Same

Номер: US20130212745A1

Автор: Nikolaj Spodsberg

Принадлежит: Novozymes AS, Novozymes Inc

The present invention relates to isolated polypeptides having endoglucanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

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Номер записи: 50

22-08-2013 дата публикации

PRODUCTION OF XYLITOL FROM A MIXTURE OF HEMICELLULOSIC SUGARS

Номер: US20130217070A1

Автор: Nair Nikhil Unni, Racine Michael, Woodyer Ryan, Zhao Huimin

Принадлежит:

Materials and methods are described to produce xylitol from a mixture of hemicellulosic sugars by several routes. Examples include either as a direct co-product of a biorefinery or ethanol facility, or as a stand-alone product produced from an agricultural or forestry biomass feedstock including using, e.g. ethanol waste streams. 1. A method to produce xylitol from a mixture of hemicellulosic sugars , the method comprising treating the mixture of hemicellulosic sugars with enzymes produced by mutant or genetically engineered microorganisms , wherein the enzymes facilitate xylitol production at an increased purity.2. The method to produce xylitol of claim 1 , comprising converting xylose alone to xylitol by the action of a xylose reductase enzyme.3. The method to produce xylitol of claim 1 , comprising conversion of L-arabinose to xylitol and reducing xylose.4. The method to produce xylitol of claim 1 , comprising reducing -xylose and metabolizing arabinose.5. A microorganism capable of converting a mixture of sugars claim 1 , wherein the sugars are selected from the group consisting of xylose claim 1 , arabinose and combinations thereof claim 1 , and wherein conversion is by fermentation to xylitol with little or no arabitol present in the final fermentation broth.6E. coliEcoli. The microorganism of is an strain that efficiently produces xylitol from -xylose claim 5 , using a mutant xylose reductase (XR) designated VMQCI and a genetically engineered -that utilizes L-arabinose as a carbon source claim 5 , thereby decreasing L-arabinotol production claim 5 , and wherein xylitol is produced at a purity of approximately 100% from an equivalent mixture of -xylose claim 5 , L-arabinose claim 5 , and -glucose claim 5 , and wherein there is a minimal production of L-arabinitol byproduct.7E. coli. The microorganism of claim 6 , wherein the strain is designated HZ 1434.8. The microorganism of wherein arabitol is less than 10% of the final mixture of polyol products produced.9 ...

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Номер записи: 51

22-08-2013 дата публикации

Method of producing biofuel using brown algae

Номер: US20130217075A1

Автор: Byung Jo Yu, Hwa Young Cho, Jae Chan Park, Jae Hwa Lee, Sung Min Park

Принадлежит: SAMSUNG ELECTRONICS CO LTD

In a method of producing biofuel using brown algae, Bacterium antarctica is used as a hydrolysis catalyst for saccharification to obtain monosaccharides from the brown algae. The saccharification with the hydrolysis catalyst is effective in saccharification of the brown algae.

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Номер записи: 52

22-08-2013 дата публикации

Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same

Номер: US20130217078A1

Автор: Christian Isak Jorgensen, Junxin Duan, Lan Tang, Randall Kramer, Ye Liu, Yu Zhang

Принадлежит: Novozymes AS, Novozymes Inc

The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

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Номер записи: 53

22-08-2013 дата публикации

Polypeptides Having Cellulolytic Enhancing Activity And Polynucleotides Encoding Same

Номер: US20130219567A1

Автор: Kirk Schnorr, Randall Kramer

Принадлежит: Novozymes AS, Novozymes Inc

The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

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Номер записи: 54

29-08-2013 дата публикации

COMPOSITE COMPONENTS FROM ANAEROBIC DIGESTED FIBROUS MATERIALS

Номер: US20130224803A1

Автор: Dvorak Stephen W., Hunt John F.

Принадлежит:

The invention relates to composite components and methods of producing composite components. In yet another embodiment, the present invention relates to a method of producing a composite component using anaerobically digested biomass. In still yet another embodiment, the method further comprises using liquid effluent from the digester. In still yet another embodiment, the method further comprises wet-mat forming and cold pressing the anaerobically digested biomass and wet-mat drying under heat and pressure. 1. A method for producing a composite component comprising: digesting waste fibrous material through an anaerobic digester to produce digested biomass; wet-mat forming and cold-pressing the digested biomass; and drying the formed wet-mat under heat and pressure to produce a composite component.2. The method of claim 1 , wherein the anaerobic digester employs a mixed plug flow design.3. The method of claim 1 , wherein the anaerobic digester uses a cork-screw flow path to move the waste fibrous material through the digester.4. The method of claim 1 , wherein the digested biomass comprises about 50% less carbohydrate content than the initial waste fibrous material.5. The method of claim 1 , wherein the digested biomass comprises about 20% more lignin content than the initial waste fibrous material.6. The method of claim 1 , wherein wet-mat forming uses mechanical pressure to remove free liquid from the mat.7. The method of claim 6 , wherein the moisture content of the mat is about 50-65%.8. The method of claim 1 , wherein drying is performed at temperatures between 380 to 420° F.9. The method of claim 1 , wherein the drying is performed under constant pressure of 60 to 1000 psi.10. The method of claim 1 , wherein drying the formed wet-mat induces fiber-to-fiber binding that is enhanced by denatured proteins.11. The method of claim 1 , wherein the method further comprises mixing a cellulosic fiber with the digested biomass during wet-mat forming claim 1 , wherein the ...

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Номер записи: 55

29-08-2013 дата публикации

EXPRESSION OF STEADY STATE METABOLIC PATHWAYS

Номер: US20130224804A1

Автор: Knight Eric

Принадлежит:

The present disclosure pertains to a method for increasing the production of a desired product having: identifying a steady state metabolic pathway for the synthesis of a desired product from a desired substrate; producing a polynucleotide encoding one or more polypeptide that participates in the steady state metabolic pathway for the synthesis of the desired product from the desired substrate; introducing the polynucleotide encoding a polypeptide into a host cell; transforming a host cell with an expression vector having an expressible polynucleotide encoding a polypeptide; and cultivating the host cell under a culture condition that induces the production of the desired product. 1. A method for increasing the production of a desired product , comprising:identifying a steady state metabolic pathway for the synthesis of a desired product from a desired substrate;producing a polynucleotide encoding one or more polypeptide that participates in the steady state metabolic pathway for the synthesis of the desired product from the desired substrate;introducing the polynucleotide encoding a polypeptide into a host cell; transforming a host cell with an expression vector comprising an expressible polynucleotide encoding a polypeptide; andcultivating the host cell under a culture condition that induces the production of the desired product.2. The method of claim 1 , further comprising collecting the desired product from the host cell.3. The method of claim 1 , wherein the desired product is glucose.4. The method of claim 1 , wherein the desired substrate is 3-Hydroxypropionic acid.5Escherichia coli.. The method of claim 1 , wherein the host cell is6. The method of claim 1 , wherein the host cell comprises a polynucleotide for T7 RNA polymerase.7. The method of claim 1 , wherein the one or more polypeptides have a sequence selected from the group consisting of SEQ ID NO: 39 claim 1 , 40 claim 1 , 41 claim 1 , 50 claim 1 , 51 claim 1 , 56 claim 1 , 57 claim 1 , 58 claim 1 , 59 ...

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Номер записи: 56

29-08-2013 дата публикации

System and Method for Converting Cellulosic Biomass into a Sugar Solution

Номер: US20130224805A1

Автор: Arnold Richard, Ceckler William H., Dumont Rino P., Hargreaves James Alan, St. Pierre James, Waite Darrell M.

Принадлежит:

A process and apparatus for converting cellulosic biomass pulp into a sugar solution is provided. The process includes combining a quantity of cellulosic biomass pulp with a quantity of acid and a quantity of enzyme. The combined quantity of cellulosic biomass pulp, enzyme, and acid are placed in an enzymatic hydrolysis reactor having a predetermined temperature range and predetermined pH level, thereby producing a quantity of monomeric sugar solution. 1. A process for converting cellulosic biomass pulp suspension into a sugar solution , the process comprising the steps of:combining a quantity of cellulosic biomass pulp suspension with a quantity of acid and a quantity of enzyme; andplacing the combined quantity of cellulosic biomass pulp, enzyme, and acid suspension in an enzymatic hydrolysis reactor having a predetermined temperature range and predetermined pH level, thereby producing a quantity of monomeric sugar solution.2. The process of claim 1 , further comprising the step of treating a quantity of cellulosic biomass to produce the quantity of cellulosic biomass pulp suspension.3. The process of claim 2 , wherein the step of treating the quantity of cellulosic biomass further comprises the step of:separating a quantity of lignin and a quantity of fiber from the quantity of cellulosic biomass, thereby producing a quantity of cellulosic pulp; andfiltering and washing the quantity of cellulosic pulp to remove a quantity of spent cooking chemicals and to thereby producing the quantity of cellulosic biomass pulp suspension.4. The process of claim 3 , wherein the separating the quantity of lignin and the quantity of fiber from the quantity of cellulosic biomass further comprises a pulping process utilizing a combination of heat and at least one chemical within a chemical pulp-cooking vessel.5. The process of claim 4 , wherein the pulping process further comprises a chemical pulping process claim 4 , wherein the chemical pulping process includes at least one process ...

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Номер записи: 57

12-09-2013 дата публикации

Methods for Producing a Fermentation Product from Lignocellulose-Containing Material

Номер: US20130236933A1

Автор: Feng Xu, Haiyu Ren, HONGZHI HUANG, Ye Chen, Yun Wang

Принадлежит: China Petroleum and Chemical Corp, Cofco Corp, Novozymes AS

The present invention provides a method for producing a fermentation product from lignocellulose-containing material, a method for converting lignocellulose-containing material into a hydrolyzate comprising mono- and oligo-saccharides, and a method for treating lignocellulose-containing material, all of which comprise the step of mixing an acid pre-treated lignocellulose-containing material and an alkaline pre-treated lignocellulose-containing material. The present invention further provides a fermentation product made according to the method for producing a fermentation product.

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Номер записи: 58

19-09-2013 дата публикации

METHOD FOR SUBJECTING SOLID BIOMASS TO SACCHARIFICATION PRETREATMENT, APPARATUS THEREFOR, AND METHOD FOR SACCHARIFICATION OF SOLID BIOMASS

Номер: US20130244284A1

Автор: ARAI Takamitsu, Fukunaga Tetsuya, Kosugi Akihiko, MACHIDA Masashi, MORI Yutaka, Morimitsu Kozo, MURATA Yoshinori, Sakashita Shigeru, Torikata Yasuo

Принадлежит:

Solid biomass having a moisture content in a range of 30 to 95 mass % is brought into contact with ammonia gas at a pressure in a range of 0.5 to 4 MPa and held at 50 to 200 degrees C. Subsequently, the solid biomass is rapidly decompressed to be blasted. The ammonia gas evaporated by decompression is recovered and recycled. The ammonia gas can be brought into contact with the entire solid biomass evenly and in a short time. Since absorption heat of the ammonia gas is generated, energy consumption required for additional heating can be reduced. 1. A saccharification pretreatment method of solid biomass , comprising:contacting moisture-containing solid biomass with ammonia gas at a pressure higher than atmospheric pressure to dissolve the ammonia gas into moisture of the solid biomass;decompressing the solid biomass contacted with the ammonia gas to a pressure lower than a pressure in the contacting with the ammonia gas to evaporate the ammonia gas dissolved in the moisture of the solid biomass; andseparating to recover the ammonia gas evaporated in the decompressing from the solid biomass.2. The saccharification pretreatment method of solid biomass according to claim 1 , whereinin the contacting of the solid biomass with the ammonia gas,the solid biomass is fed into a pressure vessel,the ammonia gas is compressed and supplied into the pressure vessel in which the solid biomass is fed, andthe solid biomass and the ammonia gas are contacted with each other at a pressure higher than atmospheric pressure.3. The saccharification pretreatment method of solid biomass according to claim 1 , whereinthe solid biomass has a moisture content in a range of 30 mass % to 95 mass %.4. The saccharification pretreatment method of solid biomass according to claim 1 , whereinthe ammonia gas has a moisture content of 10 mass % or less.5. The saccharification pretreatment method of solid biomass according to claim 1 , whereinin the contacting of the solid biomass with the ammonia gas, ...

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Номер записи: 59

19-09-2013 дата публикации

COSMETIC BIO-CELLULOSE MASK PACK SHEET AND METHOD FOR MANUFACTURING SAME

Номер: US20130244977A1

Автор: CHO Jun Cheol, Han Sang Hoon, Hsu Kuan Chi, Kim Youn Joon, Lee Chang Keun

Принадлежит: AMOREPACIFIC CORPORATION

The present invention relates to a mask pack sheet using bio-cellulose obtained from fermented ginseng extracts, and more particularly, to a method for manufacturing a cosmetic mask pack sheet, comprising injecting microbial strains into a culture medium containing ginseng extracts so as to ferment the ginseng extracts and prepare a bio-cellulose sheet, and dipping the sheet into a cosmetic liquid. 1. A cosmetic mask pack sheet comprising a bio-cellulose produced in a culture medium containing a ginseng extract.2. The cosmetic mask pack sheet of claim 1 , wherein the ginseng extract is contained in an amount of 0.01-100 wt % based on the total weight of the culture medium.3. The cosmetic mask pack sheet of claim 1 , wherein the culture medium further contains yeast extract or ammonium sulfate as a nitrogen source.4. The cosmetic mask pack sheet of claim 1 , wherein the bio-cellulose mask pack sheet contains water in an amount corresponding to 1-50 times the dry weight of the bio-cellulose mask pack sheet claim 1 , before it is impregnated with a cosmetic emulsion.5. A method for manufacturing a cosmetic mask pack sheet claim 1 , the method comprising the steps of:1) inoculating a bio-cellulose-producing microbial strain into a culture medium containing a ginseng extract to produce bio-cellulose; and2) killing the inoculated microbial strain and removing the culture medium. The present invention relates to a cosmetic mask pack sheet comprising a bio-cellulose obtained by fermenting a ginseng extract and to a method for producing bio-cellulose which can be used as a mask pack sheet.Generally, a sheet-type mask pack in the cosmetic field is a face-shaped sheet product which can exhibit moisturizing and cleansing effects when applied to a face without the need to apply cosmetic lotion with hand. Examples of sheet-type mask packs, which are on the market, include a sheet designed to completely cover a face, a composite sheet comprising an upper sheet and a lower sheet, ...

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Номер записи: 60

19-09-2013 дата публикации

Als inhibitor herbicide tolerant beta vulgaris mutants

Номер: US20130247253A1

Автор: Andreas Loock, Bernd Holtschulte, Clemens Springmann, Guenter Donn, Juergen Benting, Nathalie Knittel-Ottleben, Rudorf Jansen, Ruediger Hain

Принадлежит: Bayer Intellectual Property GmbH

The present invention relates to an ALS inhibitor herbicide tolerant Beta vulgaris plant and parts thereof comprising a mutation of an endogenous acetolactate synthase (ALS) gene, wherein the ALS gene encodes an ALS polypeptide containing an amino acid different from tryptophan at a position 569 of the ALS polypeptide.

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Номер записи: 61

26-09-2013 дата публикации

PROCESSING BIOMASS

Номер: US20130252283A1

Автор: Masterman Thomas Craig, Medoff Marshall

Принадлежит: XYLECO, INC.

Biomass feedstocks (e.g., plant biomass, animal biomass, and municipal waste biomass) are processed to produce useful products, such as fuels. For example, systems are described that can convert feedstock materials to a sugar solution, which can then be fermented to produce ethanol. Biomass feedstock is saccharified in a vessel by operation of a jet mixer, the vessel also containing a fluid medium and a saccharifying agent. 128-. (canceled)29. A method comprising:converting a low molecular weight sugar to a product by mixing the low molecular weight sugar with a microorganism in a fluid medium, using a jet mixer, wherein the jet mixer comprises a jet-flow agitator and the vessel has an arcuate bottom surface and a longitudinal axis of a shaft of the jet flow agitator is offset laterally from a longitudinal axis of the vessel.30. The method of wherein the fluid medium comprises water.31. The method of wherein the microorganism comprises yeast.32. (canceled)33. The method of wherein the jet mixer further comprises a jet aeration type mixer having a delivery nozzle claim 29 , and wherein converting the feedstock comprises delivering a jet through the delivery nozzle.34. The method of wherein the jet mixer further comprises a suction chamber jet mixer.35. A feedstock saccharification apparatus comprising:a tank,a feedstock delivery device configured to deliver a feedstock to the tank, anda feedstock processing agent delivery device configured to deliver a metered amount of a feedstock processing agent to the tank, anda jet mixer having a nozzle disposed within the tank and configured to mix the delivered biomass feedstock and saccharifying agent wherein the jet mixer comprises a plurality of jet-flow agitators, each jet-flow agitator being configured to operate reversibly, pumping fluid towards the top of the vessel in a first mode, and towards the bottom of the vessel in a second mode.36. The apparatus of wherein the jet mixer further comprises a motor claim 35 , and ...

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Номер записи: 62

26-09-2013 дата публикации

PROTEIN-ENHANCED SURFACTANTS FOR ENZYME ACTIVATION

Номер: US20130252287A1

Автор: BALDRIDGE John W., GOLDFELD Michael G., MICHALOW Andrew H., PODELLA Carl W.

Принадлежит: ADVANCED BIOCATALYTICS CORP.

Disclosed herein are compositions containing enzymes, particularly acting at the interface between two immiscible phases where the rate of enzymatic activity is increased by addition of a blend of surfactant(s) and a mixture derived from yeast fermentation, that contain non-enzymatic exo-proteins released by yeast in response to a non-lethal stress. The enzymes include those that work at the interface between an aqueous solution and a water immiscible phase, liquid or solid, such as oil, fat, cellulose, lignin, etc. including, but not limited to the following or combinations thereof: lipases, polysaccharase, lignase, cellulase and the like, in which the substrate of an enzymatic reaction forms a phase, segregated from the aqueous solution in which the enzymes are typically operating. Disclosed herein are methods for improving a washing solution with the use of these compositions, where the enzyme-protein-surfactant solution can be used in such applications as: laundry, spot remover, pre-laundry, dishes, hard surface cleaning, wastewater treatment, cellulose breakdown as in ethanol production, lignin utilization, environmental remediation, industrial cleaning, and agricultural applications. 1. A composition comprising non-enzymatic exo-proteins , a surfactant and an enzyme.2. The composition of claim 1 , wherein the exo-proteins are derived from yeast fermentation process.3Saccharomyces cerevisiae.. The composition of claim 2 , wherein the yeast is4. The composition of claim 2 , wherein the fermentation process is aerobic.5. The composition of claim 2 , wherein the fermenting yeast is subject to a stress condition.6. The composition of claim 5 , wherein the stress is a non-lethal heat shock.7. The composition of claim 1 , wherein the enzyme is selected from the group claim 1 , or combinations thereof: lipase claim 1 , cellulase claim 1 , lignase claim 1 , polysaccharase.8. The composition of claim 1 , wherein the surfactant is anionic claim 1 , nonionic claim 1 , ...

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Номер записи: 63

03-10-2013 дата публикации

Exopolysaccharide

Номер: US20130261071A1

Автор: Barbara Sheil, Liam OMAHONY, Raymond Alan Grant

Принадлежит: Alimentary Health Ltd, Procter and Gamble Co

An isolated polysaccharide has the structure [-β(1,3)-D-GaIpNAc-β(1,4)-D-Glcp-] n . The polysaccharide may be from a Bifidobacterium strain NCIMB 41003. The polysaccharide exhibits immunomodulatory activity.

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Номер записи: 64

17-10-2013 дата публикации

Processing biomass and petroleum containing materials

Номер: US20130273612A1

Автор: Marshall Medoff

Принадлежит: Xyleco Inc

Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful products, such as fuels. For example, systems can use feedstock materials, such as cellulosic and/or lignocellulosic materials and/or starchy materials, to produce ethanol and/or butanol, e.g., by fermentation.

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Номер записи: 65

24-10-2013 дата публикации

Method for Transforming Iota-Carrageenan Into Alpha-Carrageenan by Means of a New Class of 4S-Iota-Carrageenan Sulfatase

Номер: US20130280765A1

Автор: Genicot-Joncour Sabine, Helbert William, Prechoux Aurelie

Принадлежит: CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS

A method for transforming iota-carrageenan into alpha-carrageenan by a new class of 4S-iota-carrageenan sulfatase. The invention also relates to carrageenans obtained by the conversion method. The invention can be especially applied to the agro-food, pharmaceutical and cosmetic industries. 14-. (canceled)7. The method as claimed in either of claim 5 , in which said enzyme is a 4S-iota-carrageenan sulfatase.8. The method as claimed in claim 5 , in which the enzyme is produced by a host cell comprising a nucleic acid encoding said enzyme and/or a vector comprising a nucleic acid sequence encoding said enzyme. 1. Technical FieldThe present invention relates to a method for transforming iota-carrageenan to alpha-carrageenan by means of a novel class of 4S-iota-carrageenan sulfatase. The present invention also relates to carrageenans obtained by said conversion method.The present invention finds application especially in the agro-food, pharmaceutical and cosmetic industries.In the description below, the references in square brackets ([ ]) refer to the list of references presented at the end of the text.2. State of the ArtCarrageenans are sulfated galactans extracted from the wall of marine red algae. Carrageenans are composed of a succession of D-galactosides alternately linked by alpha(1-3) and beta(1-4) bonds. These anionic polysaccharides are mainly distinguishable by the presence or otherwise of a 3,6 anhydro bridge on the galactose residue linked at the alpha(1-3) position, and by their degree of sulfation. For example, the three disaccharide repeating units—called carrabiose motif—found in the most industrially exploited carrageenans are characterized by the presence of one (kappa-carrabiose), two (iota-carrabiose) or three sulfates (lambda-carrabiose) (). Carrageenans may be mainly composed of a carrabiose motif, for example kappa-carrageenan from the alga is composed of about 90% kappa-carrabiose motif and 10% iota-carrabiose. The iota-carrageenan extracted from ...

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Номер записи: 66

31-10-2013 дата публикации

Processing Biomass Containing Materials

Номер: US20130288307A1

Автор: Marshall Medoff

Принадлежит: Xyleco Inc

Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful products, such as fuels. For example, systems can use feedstock materials, such as cellulosic and/or lignocellulosic materials and/or starchy materials, to produce ethanol and/or butanol, e.g., by fermentation.

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Номер записи: 67

31-10-2013 дата публикации

CELLULOSE SACCHARIFICATION APPARATUS, BIOMASS SACCHARIFICATION APPARATUS, FERMENTATION APPARATUS AND CELLULOSE SACCHARIFICATION METHOD

Номер: US20130288311A1

Автор: Hara Michikazu, Kaneko Norimitsu, Kitano Makoto, Nariai Kentaro, OKA Tatsuya, Sato Kenji

Принадлежит:

A fermentation apparatus (A) of the present invention comprising: an enzymatic reactor () for degrading cellulose using a diastatic enzyme, and a first catalytic reactor () for degrading the degradation product produced by the enzymatic reactor () into glucose, using a solid acid catalyst (X). According to this fermentation apparatus (A), saccharification treatment of cellulose can be performed while reducing diastatic enzyme costs. 1. A cellulose saccharification apparatus , comprising:an enzymatic reactor for degrading cellulose using a diastatic enzyme, anda first catalytic reactor for degrading the degradation product produced by the enzymatic reactor into glucose, using a solid acid catalyst.2. The cellulose saccharification apparatus according to claim 1 , wherein the diastatic enzyme is a heat-resistant enzyme.3. A biomass saccharification apparatus claim 1 , comprising:a pressurized hot water reactor for selectively degrading hemicellulose contained in biomass by allowing pressurized hot water to act on the biomass,a solid-liquid separator for separating cellulose as a solid from a treated liquid of the pressurized hot water reactor, and{'claim-ref': {'@idref': 'CLM-00001', 'claim 1'}, 'a cellulose saccharification apparatus of for degrading cellulose separated by the solid-liquid separator into glucose.'}4. The biomass saccharification apparatus according to claim 3 , further comprising a second catalytic reactor for degrading a hemicellulose degradation product as the liquid separated by the solid-liquid separator claim 3 , into a hemicellulose-derived monosaccharide claim 3 , using a solid acid catalyst.5. A fermentation apparatus claim 3 , comprising:{'claim-ref': {'@idref': 'CLM-00004', 'claim 4'}, 'the biomass saccharification apparatus of ,'}a first fermenter for producing fermentative products from glucose produced by the biomass saccharification apparatus, anda second fermenter for producing fermentative products from a hemicellulose-derived ...

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Номер записи: 68

31-10-2013 дата публикации

TREATMENT METHOD FOR BIOMASS TO MAXIMIZE SUGAR YIELD, AND ADDITIVE USED IN SAME

Номер: US20130288312A1

Автор: Eom In Yong, Hong Kyung Sik, Jung Chan Duck, SONG Bong Keun, Yu Ju Hyun

Принадлежит: KOREA RESEARCH INSTITUTE OF CHEMICAL TECHNOLOGY

Disclosed is a treatment method of biomass to maximize sugar yield, which uses a specific additive which can effectively adsorb lignin-derived compounds and various inhibitors of the enzymatic activity to promote saccharification of cellulose catalyzed by cellulose hydrolases, and thus can maximize sugar yield from pretreated biomass. 1. A treatment method of biomass comprising the steps of:(a) suspending biomass in water or an acidic aqueous solution followed by autohydrolysis or acid pretreatment; and(b) subjecting the suspension obtained in step (a) to enzymatic saccharification,wherein an additive selected from the group consisting of natural silicate mineral, artificial silicate mineral, zirconia, heat-resistant organic polymer, activated carbon, and a mixture thereof is added in at least one step of step (a) and step (b).2. The treatment method of claim 1 , wherein the biomass is used in pulverized or powdered form.3. The treatment method of claim 2 , wherein the pulverized or powdered biomass is derived from the group consisting of: agricultural by-products comprising sunflower stalk claim 2 , corn stover claim 2 , bagasse claim 2 , palm residue claim 2 , rice straw claim 2 , barley straw and wheat straw; forest trees and by-products thereof comprising yellow poplar claim 2 , willow claim 2 , spruce; and bioenergy crops comprising miscanthus claim 2 , reed and switchgrass.4. The treatment method of claim 1 , wherein the natural silicate mineral is selected from the group consisting of diatomite claim 1 , Fuller's earth claim 1 , mica claim 1 , zeolite claim 1 , kaolinite claim 1 , talc claim 1 , pyrophyllite claim 1 , sand and a mixture thereof.5. The treatment method of claim 1 , wherein the artificial silicate mineral is a powder or granule prepared by mixing one or more of natural silicate minerals claim 1 , a sintered material prepared by molding the powders followed by sintering at high temperature claim 1 , a synthetic zeolite or a glass bead.6. The ...

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Номер записи: 69

07-11-2013 дата публикации

Processing biomass

Номер: US20130295624A1

Автор: Marshall Medoff, Thomas Craig Masterman

Принадлежит: Xyleco Inc

Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful products, such as fuels. For example, systems can use feedstock materials, such as cellulosic and/or lignocellulosic materials, to produce ethanol and/or butanol, e.g., by fermentation.

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Номер записи: 70

14-11-2013 дата публикации

Methods of directly extracting microrna from microvesicle in cell line, cell culture, or body fluid

Номер: US20130302856A1

Автор: Chang-Eun Yoo, Ga-hee KIM, Myoung-soon KIM

Принадлежит: SAMSUNG ELECTRONICS CO LTD

A method of extracting a nucleic acid from a microvesicle, the method comprising treating the microvesicle with a composition comprising a detergent and an aprotic solvent to extract a nucleic acid from the microvesicle.

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Номер записи: 71

14-11-2013 дата публикации

MULTI-STAGE PROCESS FOR PRODUCTION OF IMMUNE MODULATOR

Номер: US20130303752A1

Автор: Horst Geoffrey Paul, LeBrun Jeffrey Richard, Levine Robert Bernard

Принадлежит: Algal Scientific Corporation

Immune function of an animal can be modulated by administration of a composition that includes algae meal or beta glucan. The algae meal can be made by growing using particular methods and conditions, including certain continuous, semi-continuous, fed-batch, and repeat batch methods in sterile fermenters. provides a form of beta glucan that is different from other organisms, where the beta glucan is predominantly unbranched beta-1,3-glucan. Use of algae meal and beta glucan produced by the disclosed processes can improve the wellbeing of an animal or human, and may augment or even replace the use of antibiotics in certain circumstances. 1Euglena. A method for growing comprising:{'i': 'Euglena', 'growing heterotrophically in a growth media;'}{'i': Euglena', 'Euglena', 'Euglena, 'removing a portion of the growth media comprising to form a first removed growth media, wherein the first removed growth media has a concentration of at least about 20 grams dry weight per liter and the have greater than 30% by weight beta glucan and less than 70% by weight beta glucan; and'}replenishing a portion of the growth media with fresh growth media to form a first replenished growth media.2Euglena. The method of claim 1 , wherein the first removed growth media has a concentration of at least about 50 grams dry weight per liter.3Euglena. The method of claim 1 , wherein the in the first removed growth media have greater than about 40% by weight beta glucan and less than about 60% by weight beta glucan.4EuglenaEuglena. The method of claim 1 , further comprising growing in the first replenished growth media to a concentration of at least about 20 grams dry weight per liter.5Euglena. The method of claim 4 , further comprising removing a portion of the first replenished growth media having a concentration of at least about 20 grams dry weight per liter to form a second removed growth media.6. The method of claim 5 , further comprising replenishing a portion of the first replenished growth ...

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Номер записи: 72

21-11-2013 дата публикации

BIOSYNTHETIC FUNCTIONAL CELLULOSE (BC) FIBERS AS SURGICAL SUTURES AND REINFORCEMENT OF IMPLANTS AND GROWING TISSUE

Номер: US20130309295A1

Автор: Gatenholm Paul

Принадлежит:

Embodiments of the invention are based on the fermentation of bacteria to produce nano-cellulose in oxygen permeable tubular bioreactors. The resulting hydrogel non-hollow fiber can be stretched and dewatered to form strong, stiff yet flexible fiber. The fiber can be dehydrated by freeze drying or solvent exchange to form macroporous material and then optionally soaked with a solution of growth factors, anti-inflammatory drugs, and/or anitibacterial agents to provide a slow release drug delivery device in fiber form. The surface of the fiber is composed of nano-structured cellulose which promotes cell migration, tissue integration, and the healing process. BC fibers are not degraded in the human body and thus are well suited as reinforcement of implants and growing tissue. Uses for the BC fibers include surgical sutures, and reinforcing and promoting regeneration of damaged tissue or implants. 1. A process for producing biosynthetic cellulose (BC) fibers comprising:injecting a suspension of cellulose producing bacteria in medium for growing BC hydrogel into oxygen permeable tubing with a diameter of less than 3 mm;growing BC hydrogel in the tubing to a desired length and diameter and having a desired morphology, porosity, and mechanical properties; andstretching and drying the BC hydrogel to align cellulose nanofibrils.2. The process of further comprising combining the BC hydrogel with growth factor or anti-inflammatory drugs before drying.3. The process of further comprising surface modifying the BC hydrogel with antimicrobial agents to produce antimicrobial BC fibers.4. BC fibers formed from the process of .5. Surgical sutures formed from the BC fibers produced in .6. BC fiber produced according to for use as surgical sutures.7. A drug delivery device comprising BC fiber produced according to .8. BC fiber produced according to for use in drug delivery.9. A method reinforcing a medical implant or biomedical device comprising using BC fibers produced according to to ...

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Номер записи: 73

21-11-2013 дата публикации

Methods for Degrading or Converting Cellulosic Material

Номер: US20130309723A1

Автор: Huang Hongzhi, Ren Haiyu

Принадлежит: NOVZYMES A/S

Provided are methods for degrading or converting a cellulosic material, comprising: treating the cellulosic material with an enzyme composition in the presence of a polypeptide having catalase activity; and enzyme composition used for degrading or converting a cellulosic material comprising one or more (e.g., several) enzymes having cellulolytic and/or hemicellulolytic activity and a polypeptide having catalase activity. 125-. (canceled)26. A method for degrading or converting a cellulosic material , comprising: treating the cellulosic material with an enzyme composition in the presence of a polypeptide having catalase activity.27. The method of claim 26 , wherein the enzyme composition comprises one or more enzymes selected from the group consisting of a cellulase claim 26 , a GH61 polypeptide having cellulolytic enhancing activity claim 26 , a hemicellulase claim 26 , an esterase claim 26 , an expansin claim 26 , a laccase claim 26 , a ligninolytic enzyme claim 26 , a pectinase claim 26 , a peroxidase claim 26 , a protease claim 26 , and a swollenin.28. The method of claim 26 , wherein the cellulosic material is selected from the group consisting of agricultural residue claim 26 , herbaceous material claim 26 , municipal solid waste claim 26 , pulp and paper mill residue claim 26 , waste paper claim 26 , and wood; preferably claim 26 , arundo claim 26 , bagasse claim 26 , bamboo claim 26 , corn cob claim 26 , corn fiber claim 26 , corn stover claim 26 , miscanthus claim 26 , orange peel claim 26 , rice straw claim 26 , switchgrass claim 26 , wheat straw claim 26 , eucalyptus claim 26 , fir claim 26 , pine claim 26 , poplar claim 26 , spruce claim 26 , willow claim 26 , algal cellulose claim 26 , bacterial cellulose claim 26 , cotton linter claim 26 , filter paper claim 26 , microcrystalline cellulose claim 26 , or phosphoric-acid treated cellulose.29. The method of claim 26 , wherein the cellulosic material is pretreated claim 26 , especially by chemical ...

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Номер записи: 74

21-11-2013 дата публикации

Method and apparatus for producing cells and fat soluble materials by cell culture

Номер: US20130309757A1

Автор: Sung-Chun Kim

Принадлежит: Individual

The present invention relates to a method and apparatus for producing cells without injury and fat-soluble materials by from cell culturing in an inexpensive and highly efficient manner. The apparatus according to the present invention comprises a culturing device 10 , a solvent device 20 , a mixing device 30 , a separation device 40 , a fractionation device 50 , a cell accommodation device 60 , and a fat-soluble material solvent accommodation device 70.

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Номер записи: 75

05-12-2013 дата публикации

INTEGRATED CARBON CAPTURE AND ALGAE CULTURE

Номер: US20130319059A1

Автор: Chen Shulin, Chi Zhanyou, Xie Yuxiao, Zhao Baisuo

Принадлежит: WASHINGTON STATE UNIVERSITY

The feasibility of using COfrom a concentrated source to grow microalgae is limited by the high cost of COcapture and transportation, as well as significant COloss during algae culture. Another challenge is the inability of algae in using COduring night while COis continuously produced from the source. To address these challenges, this invention provides a process in which COis captured as bicarbonate and used as feedstock for algae culture. Then the carbonate is regenerated in the algae culture process as absorbent to capture more CO, which is converted to bicarbonate for use as feedstock, etc. This process significantly reduces carbon capture costs since it avoids the energy for carbonate regeneration. Also, transporting a solid or aqueous bicarbonate solution has a much lower cost than transporting compressed CO, and using bicarbonate provides a better alternative for COdelivery to algae culture systems than supplying COgas. 1. An integrated method culturing algae or cyanobacteria , comprising the steps of{'sub': 2', '2, 'i) capturing COfrom a source of CO;'}{'sub': '2', 'ii) converting captured COinto bicarbonate;'}iii) culturing alkaliphilic algae or alkaliphilic cyanobacteria using said bicarbonate as a carbon source to produce algal bioproducts;{'sub': '2', 'iv) using spent medium from said step of culturing as said source of COin said step of capturing; and'}v) repeating steps i) to iv).2. The method of claim 1 , wherein said bicarbonate is in a form selected from the group consisting of solid bicarbonate and a liquid bicarbonate solution.3SynechocystisCyanotheceMicrocoleusEuhalotheceSpirulina. The method of claim 1 , wherein said alkaliphilic cyanobacteria are selected from the group consisting of sp. claim 1 , sp. claim 1 , sp. claim 1 , sp. and sp.4ChlorellaDunaliella.. The method of claim 1 , wherein said alkaliphilic algae are eukaryotic microalgae selected from the group consisting of and5. The method of claim 1 , wherein culture medium used in said ...

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Номер записи: 76

05-12-2013 дата публикации

Method for industrially producing cyclic-structure-containing branched glucan

Номер: US20130323799A1

Автор: Iwao Kojima, Kouji Odan, Michiyo Yanase, Takeshi Takaha, Tsunehisa Akiyama

Принадлежит: Ezaki Glico Co Ltd

An object of the present invention is to provide a method for industrially producing a branched glucan having a cyclic structure. The method for producing a branched glucan having a cyclic structure comprises the steps of: (1) preparing a mixed liquid which contains a branching enzyme in which starch granules are suspended at a concentration of 5% by weight or more and 50% by weight or less, and allowing the branching enzyme to act on starch in the starch granules, wherein a temperature of the mixed liquid at the time of preparation is 0° C. or higher and not higher than the gelatinization starting temperature of the starch granule; and (2) elevating the temperature of the mixed liquid to 85° C. or higher and 129° C. or lower, wherein in the method, none of α-amylase, β-amylase, amyloglucosidase and a transglucosidase is added to the mixed liquid.

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Номер записи: 77

12-12-2013 дата публикации

Liquefaction biomass processing with heat recovery

Номер: US20130330782A1

Автор: Justin van Rooyen, Michael R. Ladisch, Nathan Mosier, YoungMi Kim

Принадлежит: PURDUE RESEARCH FOUNDATION

Described are processes that include the non-enzymatic, hydrolytic liquefaction of lignocellulosic biomass to form digest slurries and heat recovery from such digest slurries. Due to enhanced flow properties of the digest slurries such heat recovery can be efficiently conducted in spiral, plate and frame or other heat exchanger designs, with the recovered heat going to unit operations of the process such as heating incoming pretreatment media for the liquefaction. Processes can also involve additional hydrolytic digestion of some or all of the initial slurry components with enzyme and/or additional heat recovery from the initial slurry by direct contact heat exchange in which a portion of the digest slurry liquids is flashed to vapor and that vapor is condensed onto incoming lignocellulosic biomass to the process. Processes as described can be integrated into ethanol manufacture by fermentation of sugars from the digested compositions.

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Номер записи: 78

26-12-2013 дата публикации

Method for producing sugar solution

Номер: US20130344543A1

Автор: Hiroyuki Kurihara, Katsushige Yamada, Yuki Yamamoto

Принадлежит: TORAY INDUSTRIES INC

A method produces a sugar liquid by adding a filamentous fungus-derived cellulase to a pretreated product of cellulose to obtain a hydrolysate; adding waste molasses to said hydrolysate to obtain a mixed sugar liquid; and subjecting said mixed sugar liquid to solid-liquid separation and filtering the obtained solution component through an ultrafiltration membrane, to recover the filamentous fungus-derived cellulase as a non-permeate and to obtain a sugar liquid as a permeate.

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Номер записи: 79

26-12-2013 дата публикации

Processing biomass

Номер: US20130344586A1

Автор: Harrison Medoff, Marshall Medoff, Thomas Craig Masterman

Принадлежит: Xyleco Inc

Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed for use in the production of useful products, such as fuels. For example, systems can use biomass materials, such as cellulosic and/or lignocellulosic materials, to enhance the production of a product, e.g., the production of ethanol and/or butanol by fermentation.

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Номер записи: 80

02-01-2014 дата публикации

Processing biomass

Номер: US20140004570A1

Автор: Marshall Medoff, Michael W. Finn, Thomas Craig Masterman

Принадлежит: Xyleco Inc

Provided herein are methods of increasing the efficiency of biomass saccharification. In particular, the methods include ways of avoiding feedback inhibition during the production of useful products.

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Номер записи: 81

02-01-2014 дата публикации

PROCESSING BIOMASS

Номер: US20140004573A1

Автор: Creasey Kaitlyn, Huang Jamie K, LAVIGNE Randy, Masterman Thomas Craig, Medoff Marshall

Принадлежит:

Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful intermediates and products, such as energy, fuels, foods or materials. For example, systems are described that can use feedstock materials, such as cellulosic and/or lignocellulosic materials, to produce an intermediate or product, e.g., by enzymatic saccharification in a continuous, semi-continuous or non-continuous fashion. 1. A method comprising:separating a solid saccharified biomass from a liquid medium, andsaccharifying the solid saccharified biomass.2. The method of wherein the liquid medium comprises enzymes and sugars.3. The method of wherein the solid saccharified biomass is wetted by the liquid medium.4. The method of wherein the solid saccharified biomass and liquid medium are produced by saccharifiying a solid biomass in a liquid.5. The method of wherein the biomass has been treated by a method selected from the group consisting of irradiation claim 4 , sonication claim 4 , oxidation claim 4 , pyrolysis claim 4 , steam explosion and combinations thereof.6. The method wherein the biomass has been treated by irradiation.7. The method of wherein the biomass receives a total dosage of between about 10 and 200 Mrad.8. The method of wherein the solid saccharified biomass and liquid medium are separated by a separator selected from the group consisting of a centrifuge claim 1 , a filtering device claim 1 , a settling tank claim 1 , a porous material claim 1 , a mesh claim 1 , a strainer claim 1 , a vibratory screener claim 1 , a perforated plate or cylinder claim 1 , a sieving device and combinations of these.9. The method of wherein saccharifiying the biomass is completed.10. The method of wherein saccharifiying the biomass is at least 20% completed.11. The method of wherein the biomass is a cellulosic or lignocellulosic biomass.12. The method of wherein the biomass is selected form the group consisting of paper claim 11 , paper products claim 11 , paper ...

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Номер записи: 82

02-01-2014 дата публикации

Production of products from biomass

Номер: US20140004574A1

Автор: Aiichiro YOSHIDA, Jaewoong MOON, Marshall Medoff, Thomas Craig Masterman

Принадлежит: Xyleco Inc

The processes disclosed herein include saccharifying cellulosic and/or lignocellulosic biomass and fermenting the sugars to produce a sugar alcohol.

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Номер записи: 83

09-01-2014 дата публикации

FERMENTATION PROCESSES FOR CULTIVATING STREPTOCOCCI AND PURIFICATION PROCESSES FOR OBTAINING CPS THEREFROM

Номер: US20140011183A1

Автор: BAZZOCCHI Giulia, Berti Francesco, CICALA Concetta, Costantino Paolo, FONTANI Silvia, NORELLI Francesco, OLIVIERI Roberto

Принадлежит:

This invention is in the field of bacterial cultures and specifically relates to the optimization of culture conditions to improve the production of bacterial capsular polysaccharides from strains in fed batch culture and to novel purification methods suitable for production scale purification of bacterial capsular polysaccharides from strains resulting in higher levels of purity than previously obtained for production scale. 1StreptococcusStreptococcus. A method for cultivating for production of capsular polysaccharides (cps) on a manufacturing scale , wherein said method comprises (a) providing an inoculum of a strain of expressing the cps , and (b) cultivating the strain by fermentation , wherein said cultivating comprises a linear addition of a carbon source to a cultivating medium , and does not use an algorithm to control the cultivating by monitoring a pH of the cultivating medium.2. The method of further comprising step (c) recovering the capsular polysaccharide.3StreptococcusStreptococcus agalactiae.. The method of claim 1 , wherein said strain of further comprises4Streptococcus agalactiae. The method of claim 3 , wherein said strain of is 090 claim 3 , H36b claim 3 , CBJ111 claim 3 , or M781.5. The method of claim 1 , wherein an optical density (OD) of the inoculum is between about 0.6 and about 1.8.6. The method of claim 1 , wherein a pH of the cultivating medium is between about 6.0 and about 7.5.7. The method of claim 6 , wherein the pH is about 7.3.8. The method of claim 1 , wherein a temperature of the cultivating medium is between about 34 and about 38° C.9. The method of claim 8 , wherein the temperature is about 36° C.10. The method of claim 1 , wherein said carbon source further comprises glucose.11. The method of claim 1 , wherein said cultivating further comprises monitoring an OD of the cultivating medium such that when the OD reaches a designated level claim 1 , said linear addition of a carbon source is initiated.12. The method of claim 11 , ...

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Номер записи: 84

09-01-2014 дата публикации

Genetically Modified Microorganisms Capable of Producing Beta-Glucans and Methods for Producing Beta-Glucans

Номер: US20140011243A1

Автор: Brockmann Beata, FLECK Christian, Granström Mari, Haefner Stefan, Herold Andrea, Schmidt Julia Kristiane, Schröder Hartwig, Zelder Oskar

Принадлежит:

The present invention relates to genetically modified microorganisms capable of producing beta-glucans, characterized in that said genetically modified microorganism overexpresses (i) a polynucleotide encoding a polypeptide having 1,3-β-D-glucan synthase-activity, and/or (ii) a polypeptide having 1,3-β-D-glucan synthase-activity, compared to a corresponding non-modified control microorganism of the same strain. The present invention also relates to the use of a polynucleotide encoding a polypeptide having 1,3-β-D-glucan synthase-activity or the use of such a polypeptide for producing β-glucans. Furthermore, the present invention relates to methods for producing β-glucans comprising the introduction of a promoter upstream of a polynucleotide encoding a polypeptide having 1,3-β-D-glucan synthase-activity thereby increasing the expression of said polynucleotide, or a polynucleotide encoding a polypeptide having 1,3-β-D-glucan synthase-activity into a microorganism being able to synthesize β-glucans. 1. A genetically modified microorganism capable of producing a polymer consisting of a linear main chain of β-D-(1-3)-glucopyranosyl units having a single β-D-glucopyranosyl unit (1-6) linked to a β-D-glucopyranosyl unit of the linear main chain with an average branching degree of about 0.3 , characterized in that said genetically modified microorganism overexpresses (i) a polynucleotide encoding a polypeptide having 1 ,3-β-D-glucan synthase-activity , and/or (ii) a polypeptide having 1 ,3-β-D-glucan synthase-activity , compared to a corresponding non-modified control microorganism of the same strain.2. (canceled)3. A method of producing a polymer consisting of a linear main chain of β-D-(1-3)-glucopyranosyl units having a single β-D-glucopyranosyl unit (1-6) linked to a β-D-glucopyranosyl unit of the linear main chain with an average branching degree of about 0.3 , said method comprising the steps of:(a) introducing (i) a strong promoter upstream of a polynucleotide ...

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Номер записи: 85

16-01-2014 дата публикации

Methods of Making Nanotechnological and Macromolecular Biomimetic Structures

Номер: US20140017725A1

Автор: SUNGUROFF Alexander

Принадлежит:

The present invention is in the fields of nanotechnology and biomimetics. In particular, the present invention relates to the use of modified ribosomes to produce biomimetic structures. These biomimetic structures, also known as directed element polymers, are not produced by traditional industrial means but instead are produced by living systems comprising modified ribosomes. 2. The method of claim 1 , further comprising the step of:(c) isolating the nanotechnological or biomimetic structure.3. The method of claim 1 , wherein the structure produced has at least one dimension on a scale of nanometers.4. The method of claim 1 , wherein the conditions comprise nonphysiological conditions selected from the group consisting of elevated or reduced pressure claim 1 , elevated or reduced temperature claim 1 , elevated or reduced pH claim 1 , and combinations thereof.5. The method of claim 1 , wherein the unnatural coding material of the mixture comprises modified nucleoside bases or non-nucleoside base replacements.6. The method of claim 1 , wherein the substrate of the mixture comprises natural or unnatural amino acids claim 1 , modified natural or unnatural amino acids claim 1 , non-amino acid molecules suitable for assembly into a nanotechnological or biomimetic structure claim 1 , or combinations thereof.7. The method of claim 6 , wherein the substrate of the mixture further comprises a metal.8. The method of claim 1 , wherein the natural or unnatural factors of the mixture farther comprise an acceptor molecule selected from the group consisting of natural tRNA claim 1 , unnatural tRNA claim 1 , an acceptor molecule capable of interacting with the coding material of the mixture to assemble substrate molecules into a nanotechnological or biomimetic structure claim 1 , and combinations thereof.9. The method of claim 8 , wherein the acceptor molecule of the mixture interacts with coding material of the mixture in sequences greater or less than three bases or base ...

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Номер записи: 86

23-01-2014 дата публикации

BACTERIAL CULTURE MEDIA AND METHODS FOR THEIR PREPARATION AND USE

Номер: US20140024075A1

Автор: Hong Feng

Принадлежит: Shanghai Zhiyi Information Technology Ltd.

Provided herein are methods and compositions for bacterial cellulose production. In some embodiments, the methods and compositions involve or are made from distiller's grain. 1. A method of making culture medium for bacteria , the method comprising:providing distiller's grain from an alcohol fermentation; andmixing the distiller's grain with at least one hydrolytic catalyst, wherein a hydrolysate is formed from the distiller's grain by the hydrolytic catalyst, thereby making a culture medium.2. The method of claim 1 , further comprising collecting the hydrolysate from the mixture.3. The method of claim 2 , further comprising detoxifying the hydrolysate.4. The method of claim 1 , further comprising providing at least one of a carbon source claim 1 , a nitrogen source claim 1 , a salt source claim 1 , and a trace element source.5. The method of claim 1 , wherein the hydrolytic catalyst comprises an acid or an enzyme.6. The method of claim 1 , wherein a ratio of solids to liquids in the mixing of the distiller's grain with the at least one hydrolytic catalyst is about 1:5 (w/v) to about 1:30 (w/v).7. The method of claim 6 , wherein the distiller's grain is mixed with about 0.3% to about 0.7% w/v acid.8. The method of claim 7 , wherein the acid is mixed with the distiller's grain at about 25 to about 200 degrees Celsius for about 30 to about 80 minutes.9. (canceled)10. The method of claim 6 , wherein the distiller's grain is mixed with about 1 to about 700 U of at least one enzyme.11. The method of claim 10 , wherein the enzyme is mixed with the distiller's grain at about 25 to about 90 degrees Celsius for about 0.5 to about 48 hours.12. The method of claim 11 , wherein the enzyme is at least one of cellulase claim 11 , hemicellulase claim 11 , xylanase claim 11 , protease claim 11 , lipase claim 11 , amylase claim 11 , glucan glucohydrolase claim 11 , or glucoamylase.13. (canceled)14. (canceled)15. The method of claim 1 , wherein the distiller's grain is at least one ...

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Номер записи: 87

23-01-2014 дата публикации

Cultivation of micro-algae and application to animal feeds, environments, field crops, and waste treatment

Номер: US20140024085A1

Автор: Burton Dudley, Cleeland Warren

Принадлежит:

The present disclosure concerns producing a micro-algae product. In preferred embodiments, the method comprises collecting urine from lactating cows, mixing the collected urine with aerobically digested cow manure to form a mother liquor, fermenting the mother liquor in an algae growth tank; and forming two distinct layers of top water, including a top water layer, the top-water layer including yeast by-products and algae by-products. 1a. collecting urine from lactating cows;b. mixing said collected urine with aerobically digested cow manure to form a mother liquor;c. fermenting said mother liquor in an algae growth tank; andd. forming two distinct layers of top water, including a top water layer, said top-water layer including yeast by-products and algae by-products.. A method for producing a micro-algae product comprising: The present disclosure relates generally to the cultivation of micro-algae and its use and application for animals, especially ruminant animals.This application claims priority to US non-provisional application Ser. No. 12/001,773, filed Dec. 11, 2007.It is well-known in commercial practice to grow and harvest micro-algae. Some of these formulations are to improve human health; some are to reduce odor; and some are for the purposes of treating waste lagoons. However, none of these systems involve the separation and collection of “top water” as the concentrated polysaccharide by-product of the algae production. Further, none of the prior systems apply this “top water” to the feeding of ruminants, as opposed to feeding the algae directly to humans or animals.In short, existing systems for using micro-algae in ruminant feeds and the allied uses are non-existent, not effective, or too expensive. This invention resolves these problems by providing a cost-effective, predictable, controlled system for the production and use of algal by-products to achieve substantial health and economic goals.Embodiments of the present invention described in the ...

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Номер записи: 88

06-02-2014 дата публикации

Integrated wood processing and sugar production

Номер: US20140038244A1

Автор: Arunas Chesonis, Jerry W. Horton

Принадлежит: Sweetwater Energy Inc

Provided is a system for localizing and optimizing harvesting of woody biomass by providing a plurality of portable pretreatment units at harvesting or mill sites. The sugars derived from woody waste products can be transported to plants for processing or processing directly by plants located at mill sites to generate biofuels and other chemicals.

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Номер записи: 89

06-02-2014 дата публикации

EXTRACELLULAR ALDONOLACTONASE

Номер: US20140038245A1

Автор: BEESON, DOUDNA CATE James H., IV William T., MARLETTA Michael A.

Принадлежит: The Regents of the University of California

The present disclosure relates to hydrolysis of hexose-δ-lactones by use of an extracellular aldonolactonase. In particular the present disclosure relates to compositions including a extracellular aldonolactonase and methods of use thereof. 1. A method of producing aldonic acid comprising contacting a hexose-δ-lactone substrate with a recombinant polypeptide comprising a polypeptide having at least 90% sequence identity to SEQ ID NO: 2 wherein said polypeptide has lactonase activity.2. The method of claim 1 , wherein the recombinant polypeptide comprises a polypeptide having at least 95% sequence identity to SEQ ID NO: 2.3. The method of claim 1 , wherein the recombinant polypeptide comprises a polypeptide having at least 99% sequence identity to SEQ ID NO: 2.4. The method of claim 1 , wherein the recombinant polypeptide comprises SEQ ID NO: 2.5. The method of claim 1 , wherein the hexose-δ-lactone substrate is cellobiono-δ-lactone.6. The method of claim 1 , wherein the hexose-δ-lactone substrate is glucono-δ-lactone.7. The method of claim 1 , wherein the hexose-δ-lactone substrate is lactono-δ-lactone.8. The method of claim 2 , wherein the hexose-δ-lactone substrate is cellobiono-δ-lactone.9. The method of claim 2 , wherein the hexose-δ-lactone substrate is glucono-δ-lactone.10. The method of claim 2 , wherein the hexose-δ-lactone substrate is lactono-δ-lactone.11. The method of claim 3 , wherein the hexose-δ-lactone substrate is cellobiono-δ-lactone.12. The method of claim 3 , wherein the hexose-δ-lactone substrate is glucono-δ-lactone.13. The method of claim 3 , wherein the hexose-δ-lactone substrate is lactono-δ-lactone.14. The method of claim 4 , wherein the hexose-δ-lactone substrate is cellobiono-δ-lactone.15. The method of claim 4 , wherein the hexose-δ-lactone substrate is glucono-δ-lactone.16. The method of claim 4 , wherein the hexose-δ-lactone substrate is lactono-δ-lactone. This application is a continuation of U.S. patent application Ser. No. 13/813, ...

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Номер записи: 90

06-02-2014 дата публикации

Processing biomass

Номер: US20140038251A1

Автор: Marshall Medoff, Michael W. Finn, Thomas Craig Masterman

Принадлежит: Xyleco Inc

Fructose, e.g., fructose derived from a cellulosic or lignocellulosic material, is use, e.g., fermented to produce a product, e.g., a solvent.

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Номер записи: 91

13-02-2014 дата публикации

USE OF VINASSE IN THE PROCESS OF SACCHARIFICATION OF LIGNOCELLULOSIC BIOMASS

Номер: US20140045237A1

Автор: Andrade Liliane Pires, Galvao Célia Maria Araújo, Neto Oswaldo Godoy, Teodoro Juliana Conceição, Tome Jose Augusto Travassos Rios

Принадлежит: CTC - CENTRO DE TECNOLOGIA CANAVIEIRA S.A.

The use of stillage in a saccharification/fermentation process of lignocellulosic biomasses, regardless of the form of the biomasses and regardless of the use of the obtained final hydrolyzed broth. The beneficial effect conferred by the stillage to the saccharification process of lignocellulosic biomasses presents among other characteristics the ability to buffer the reaction medium, especially when such process takes place in an enzymatic route, but not limited to it, regardless of the type of biomass being used and the type of pretreatment to which the biomass is subjected. Fermentation processes, such as the ethanol production, using stillage as source of nutrients such as, for example, nitrogen, for growing microorganisms, but not limited to it. 1. An enzymatic hydrolysis process comprising using stillage as a buffering agent of a reaction medium containing lignocellulosic vegetal biomass.2. The process according to claim 1 , wherein the added amount of stillage varies claim 1 , in mass percentage (% w/w) claim 1 , from 0.1% to 100% and the amount of water varies claim 1 , in mass percentage (% w/w) claim 1 , from 100% to 0%.3. The process according to claim 1 , wherein the stillage is a residue derived from a distillation process of wine without yeasts claim 1 , obtained after fermentation and separation of yeast cells.4. The process according to claim 3 , further comprising filtering claim 3 , evaporating claim 3 , of concentrating the stillage.5Lolium, Spartina, Panicum, Miscanthus. The process according to claim 1 , wherein the lignocellulosic vegetal biomass is selected from the group consisting of: herbaceous biomass; C4 plants from genera claim 1 , and combinations thereof; sugar cane bagasse (from the mill and/or diffuser); sugar cane straw; cereal straw selected from the group comprising wheat claim 1 , rice claim 1 , rye claim 1 , barley claim 1 , oat claim 1 , maize claim 1 , switchgrass claim 1 , and the similar and combinations thereof; wood claim ...

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Номер записи: 92

13-02-2014 дата публикации

Mutant cells for protein secretion and lignocellulose degradation

Номер: US20140045243A1

Автор: Elizabeth A. Znameroski, James H. Doudna Cate, N. Louise Glass

Принадлежит: UNIVERSITY OF CALIFORNIA

The present disclosure provides mutant cells for the secretion of proteins and for the degradation of lignocellulosic biomass. Methods for the use of these cells are also provided. Specifically, the utility of combined genetic deletions of β-glucosidases and the catabolite repressor gene creA/cre-1 for protein secretion in fungal and yeast cells is disclosed.

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Номер записи: 93

20-02-2014 дата публикации

Process for Production of Microalgae, Cyanobacteria and Metabolites Thereof

Номер: US20140051131A1

Автор: Bashir Nazir, Dodd John, Marsalek Blahsolov, Vosatka Miroslav

Принадлежит: Algaecytes Limited

The present invention relates to processes for the production of microalgae, cyanobacteria and/or metabolites thereof. Described herein is a process involving, the use of a stimulus applied to a microalgal or cyanobacterial culture to enhance the production of one or more metabolites. Also described herein, is a process for the production of microalgae and/or cyanobacteria comprising an adaptation stage wherein an algaltcyanobacterial culture is grown on a process water feedstock and/or under light emitting diodes (LEDs) emitting light within the spectrum of light wavelengths between around 400 nm and 700 nm, and a production phase, wherein the microalgae or cyanobacteria are grown on the same process water feedstock and/or under the same light conditions used in the adaptation stage. The invention also relates to specific microalgal strains. 1105.-. (canceled)106. A process for the enhanced production of one or more metabolites in microalgae and/or cyanobacteria , said process comprising the steps of:(i) culturing a microalgal or cyanobacterial strain through a production phase;{'sup': '2', '(ii) exposing the microalgal or cyanobacterial culture to a stimulus, wherein the stimulus comprises (a) a decrease in pH to a pH of no more than around pH 6, followed by an increase in pH to a pH of no less than around pH 7 and (b) an increase in light irradiance to at least 400 μmol/msec.'}107. The process of wherein the stimulus comprises one or more of:(a) a decrease in pH from a pH of between around pH 7 and pH 9 to a pH of between around pH 5 and pH 6;{'sup': 2', '2, '(b) an increase in LED-delivered irradiance from between around 50-200 μmol/m/sec to between around 400-2000 μ/msec.'}108. The process of wherein one or more of the following applies to the production phase:(a) the production phase corresponds to the exponential phase of growth;(b) the production phase involves growth of the microalgal or cyanobacterial strain under conditions that permit exponential growth ...

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Номер записи: 94

27-02-2014 дата публикации

Method for processing vegetable biomass

Номер: US20140053827A1

Автор: Celia Maria Araujo Galvao, Dionisio Fabiano Pegoretti, Dionisio Morelli Filho, Henrique Macedo Baudel, Jaime Fingerut, Jose Augusto Travassos Rios Tome, Liliane Pires Andrade, Osawaldo Godoy Neto

Принадлежит: CTC-Cento de Tecnologia Canavieira SA

The present invention relates to an energy-efficient process for the treatment of plant biomass, particularly sugar cane, for the production of carbohydrates and ethanol, using physico-chemical and extraction techniques, as well as very simple milling configurations, thereby minimizing energy consumption during extraction of the cane juice. The biomass treated and obtained through this process, when subjected to a fermentation process for the production of ethanol, increases the yield of the process in comparison with that of traditional sugar cane. It can also be used for the production of enzymes, animal feedstuffs, and other useful products.

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Номер записи: 95

27-02-2014 дата публикации

ENHANCED CELLODEXTRIN METABOLISM

Номер: US20140057323A1

Автор: DOUDNA CATE James H., GALAZKA Jonathan M., HA Suk-Jin, JIN Yong-Su

Принадлежит:

The present disclosure relates to host cells containing two or more of a recombinant cellodextrin transporter, a recombinant cellodextrin phosphorylase, a recombinant β-glucosidase, a recombinant phosphoglucomutase, or a recombinant hexokinase; and to methods of using such cells for degrading cellodextrin, for producing hydrocarbons or hydrocarbon derivatives from cellodextrin, and for reducing ATP consumption during glucose utilization. 1. A method for degrading cellodextrin , comprising:a) providing a host cell comprising a recombinant cellodextrin transporter and a recombinant polypeptide comprising Y-x(2)-G-x-[KR]-E-N-[AG]-[AG]-[IV]-F-x(2)-[ANST]-[NST]-x(2)-[AIV]-x(2)-[AGT]-x(4)-[AG]-x(4)-[ADNS] (SEQ ID NO: 233), Y-Q-[CN]-M-[IV]-T-F-[CN]-[FILMV]-[AS]-RS-[ST]-[AS]-S-[FY]-[FY]-E-[STV]-G-x-[GS]-R-G-[IM]-G-F-R-D-S-[ACNS]-Q-D-[ILV]-[ILMV]-G-x-V-H-x-[IV]-P-[ADEST]-x-[AV]-[KR]-[AEQ]-x-[IL]-[FIL]-D (SEQ ID NO: 14), or G-x(2)-[FY]-x-N-[AGS]-x-[AS]-W-[APS]-V-[IL]-[AS]-x(2)-A-x(2)-[DE]-x-[AI]-x(3)-[LMV]-[DEN]-[ASV]-[ILV]-x(3)-L-x-T-x(2)-G-[ILV]-x(2)-[SV]-x-P-[AG] (SEQ ID NO: 15), wherein the recombinant polypeptide has cellodextrin phosphorylase activity; andb) culturing the host cell in a medium comprising cellodextrin or a source of cellodextrin, whereby cellodextrin is transported into the cell and degraded by said recombinant polypeptide.2. (canceled)3. (canceled)4. The method of claim 1 , wherein the recombinant polypeptide comprises an amino acid sequence that has at least 29% claim 1 , at least 30% claim 1 , at least 35% claim 1 , at least 40% claim 1 , at least 45% claim 1 , at least 50% claim 1 , at least 55% claim 1 , at least 60% claim 1 , at least 65% claim 1 , at least 70% claim 1 , at least 75% claim 1 , at least 80% claim 1 , at least 85% claim 1 , at least 90% claim 1 , at least 95% claim 1 , at least 99% claim 1 , or at least 100% amino acid identity to the amino acid sequence of CDP_Clent claim 1 , CDP_Ctherm claim 1 , or CDP_Acell.5. The method of claim ...

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Номер записи: 96

13-03-2014 дата публикации

ENHANCED CELLULOSE DEGRADATION

Номер: US20140073012A1

Автор: BEESON, DOUDNA CATE James H., IV William T., MARLETTA Michael A., Phillips Christopher M.

Принадлежит: The Regents of the University of California

The present disclosure provides compositions and methods related to the degradation of cellulose and cellulose-containing materials. CDH-heme domain polypeptides and GH61 polypeptides and related polynucleotides and compositions are provided herein. Additionally, methods related to CDH-heme domain polypeptides, GH61 polypeptides, and related polynucleotides and compositions, are provided herein 14-. (canceled)5. A composition comprising:a recombinant GH61 polypeptide; anda recombinant CDH-heme domain polypeptide comprising a cellulose binding module (CBM).6. (canceled)7. The composition of claim 5 , wherein the recombinant GH61 polypeptide comprises the amino acid sequence of SEQ ID NO: 24 or SEQ ID NO: 30.8. The composition of claim 5 , wherein the recombinant GH61 polypeptide comprises the amino acid sequence of SEQ ID NO: 26 claim 5 , SEQ ID NO: 28 claim 5 , or SEQ ID NO: 90.9. The composition of claim 5 , wherein the recombinant GH61 polypeptide comprises the motif H-X-Q-X-Y.10. The composition of claim 5 , wherein the recombinant CDH-heme domain polypeptide comprises the amino acid sequence of SEQ ID NO: 32 or SEQ ID NO: 46.11. The composition of claim 5 , wherein the recombinant CDH-heme domain polypeptide comprises a first domain and a second domain claim 5 , wherein the first domain comprises a CDH-heme domain and the second domain comprises a CBM claim 5 , and wherein the polypeptide does not contain a dehydrogenase domain.12. The composition of claim 5 , wherein the recombinant CDH-heme domain polypeptide comprises a first domain claim 5 , a second domain claim 5 , and a third domain claim 5 , wherein the first domain comprises a CDH-heme domain claim 5 , the second domain comprises a CBM claim 5 , and the third domain comprises a dehydrogenase domain.1315-. (canceled)16. The composition of claim 5 , wherein the CDH-heme domain comprises the amino acid sequence selected from the group consisting of SEQ ID NO: 70 claim 5 , SEQ ID NO: 76 claim 5 , SEQ ID NO: ...

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Номер записи: 97

13-03-2014 дата публикации

Biotechnological Production of Chondroitin

Номер: US20140073772A1

Автор: Antonio Trilli, Francesca Bagatin, Immacolata Busiello, Simona Daly

Принадлежит: GNOSIS SPA

Chondroitin is produced by culturing a recombinant microorganism which is obtained by inactivation of a gene encoding an enzyme responsible for addition of fructose residues to the linear chondroitin polysaccharide in a microorganism producing a fructosylated derivative of chondroitin.

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Номер записи: 98

20-03-2014 дата публикации

NOVEL ISOLATED BACTERIAL STRAIN OF GLUCONACETOBACTER OBOEDIENS AND AN OPTIMIZED ECONOMIC PROCESS FOR MICROBIAL CELLULOSE PRODUCTION THEREFROM

Номер: US20140080184A1

Автор: Jahan Firdaus, Saxena Rajendra Kumar

Принадлежит: COUNCIL OF SCIENTIFIC & INDUSTRIAL RESEARCH

The present invention provides a novel and potent cellulose producing bacterial species, which was isolated from mixed fruit residue deposited at MTCC, IMTECH, Chandigarh under the deposition number MTCC 5610. The process for the production of microbial cellulose by this bacterium was optimized and thus, an efficient and economic process for producing high titres of microbial cellulose was developed. Further, a novel and improved method for drying of microbial cellulose has been developed wherein the microbial cellulose mats were dried using a wooden plank and porous fabric as a base at room temperature. The microbial cellulose production was successfully scaled up to 5 liters volume of production medium in trays. The present invention also recites the production and optimization of microbial cellulose in different shapes and sizes (gloves and vessels) which will be of great help for burn and injured persons/patients. 1Gluconacetobacter oboediens. An isolated , bacterial strain of having accession number MTCC 5610 , wherein the said strain being deposited at the Microbial Type Culture Collection , MTCC , Chandigarh , India a depository recognized under the Budapest Treaty.2. The strain as claimed in claim 1 , wherein it is a potent microbial cellulose producer.3. The strain as claimed in claim 1 , wherein it is isolated from mixed fruit residue mixed with sugar and water in the ratio of 1 to 3:0.1 to 0.5:2 to 4 respectively followed by incubating at a temperature of 25 to 35 degree C. for 10 to 15 days under static culture conditions.4. An optimized economic process for the production of microbial cellulose using the isolated bacterial strain as claimed in claim 1 , wherein the said process comprising the following steps:{'i': 'Gluconacetobacter oboediens', 'a) growing the bacterial isolate of MTCC 5610 under aerobic and static culture conditions in the production medium comprising 0.1 to 20.0% of a carbon source; 0.5 to 8.0% of a nitrogen source; 0.1 to 0.5% of ...

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Номер записи: 99

27-03-2014 дата публикации

ALPHA-AMYLASE BLEND FOR STARCH PROCESSING AND METHOD OF USE THEREOF

Номер: US20140087429A1

Автор: Nedwin Glenn E., Sharma Vivek, Shetty Jayarama K.

Принадлежит: DANISCO US INC.

The present disclosure relates to an enzyme blend comprising a low pH, thermostable alpha-amylase and a alpha-amylase. The blend can include at least about 1.0 Liquefon Unit (LU) of the alpha-amylase for every 5.0 Modified Wohlgemuth Unit (MWU) of the low pH, thermostable alpha-amylase. The enzyme blend described is suitable for starch liquefaction and saccharification, ethanol production, and/or sweetener production, among other things. Also provided herein is a method of processing a starch by liquefying the starch with the low pH, thermostable alpha-amylase and the alpha-amylase, simultaneously or sequentially. 1Bacillus licheniformisB. licheniformisB. licheniformis. An enzyme blend for processing a starch comprising a low pH , thermostable alpha-amylase and a alpha-amylase , wherein the low pH , thermostable alpha-amylase has an amino acid sequence that is at least about 80% identical to SEQ ID NO: 2 , wherein the alpha-amylase has an amino acid sequence that is at least about 80% identical to SEQ ID NO: 6 , and wherein the enzyme blend contains at least about 0.5 to about 5.0 Liquefon Units (LUs) of the alpha-amylase for every 5.0 Modified Wohlgemuth Units (MWUs) of the low pH , thermostable alpha-amylase.23-. (canceled)4B. licheniformisB. licheniformis. The enzyme blend of claim 1 , wherein the alpha-amylase is a variant having one or more altered properties compared to the alpha-amylase having an amino acid sequence of SEQ ID NO: 6 claim 1 , wherein the one or more altered properties include: substrate specificity claim 1 , substrate binding claim 1 , substrate cleavage pattern claim 1 , thermal stability claim 1 , pH activity profile claim 1 , pH stability profile claim 1 , stability towards oxidation claim 1 , stability at lower levers of calcium ion (Ca2+) claim 1 , specific activity claim 1 , or any combination thereof.5. The enzyme blend of claim 1 , wherein the low pH claim 1 , thermostable alpha-amylase comprises an amino acid sequence of SEQ ID NO: 2. ...

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Номер записи: 100

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