09-05-2013 дата публикации
Номер: US20130116126A1
This invention generally relates to natural gas and methylotrophic energy generation, bio-generated fuels and microbiology. In alternative embodiments, the invention provides nutrient amendments and microbial compositions, e.g., consortia, that are both specifically optimized to stimulate methanogenesis, or for “methylotrophic” or other conversions. In alternative embodiments, the invention provides methods to develop nutrient amendments and microbial compositions that are both specifically optimized to stimulate methanogenesis in a given reservoir. The invention also provides methods for the evaluation of potentially damaging biomass formation and scale precipitation resulting from the addition of nutrient amendments. In other embodiments, the invention provides methods for simulating biogas in sub-surface conditions using a computational model. 1. A composition , a fluid , a bioreactor , a mud , a reservoir , a product of manufacture or a synthetic consortium comprising:(1) a plurality of microorganism strains, (a) at least two, three, four, five, six, seven, eight, nine, ten or eleven or all twelve of the microorganism strains of Consort-ABS1; or', '(b) a group (or “consortium”) of different microorganism strains comprising at least two, three, four, five, six, seven, eight, nine, ten, eleven or twelve different microorganism strains, each strain comprising at least one 16S rRNA gene or nucleic acid sequence selected from the group consisting of a nucleic acid having at least about 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or 100% (complete) sequence identity to SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:8, SEQ ID NO: 10, SEQ ID NO: 11 and SEQ ID NO: 12, or', '(c) a group (or “consortium”) of different microorganism strains consisting of at least two, three, four, five, six, seven, eight, nine, ten, eleven or twelve different microorganism strains, each strain comprising at least one ...
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