29-09-2016 дата публикации
Номер: US20160281112A1
Автор:
Anthony R. CALABRIA,
Marguerite A. CERVIN,
Gopal K. CHOTANI,
Richard LA DUCA,
Joseph C. MCAULIFFE,
Michael C. MILLER,
Timothy A. SABO,
Karl J. SANFORD,
Erin L. WEBSTER,
Gregory M. WHITED,
CALABRIA ANTHONY R,
CERVIN MARGUERITE A,
CHOTANI GOPAL K,
LA DUCA RICHARD,
MCAULIFFE JOSEPH C,
MILLER MICHAEL C,
SABO TIMOTHY A,
SANFORD KARL J,
WEBSTER ERIN L,
WHITED GREGORY M,
CALABRIA Anthony R.,
CERVIN Marguerite A.,
CHOTANI Gopal K.,
LA DUCA Richard,
MCAULIFFE Joseph C.,
MILLER Michael C.,
SABO Timothy A.,
SANFORD Karl J.,
WEBSTER Erin L.,
WHITED Gregory M.
Принадлежит:
The invention features methods for producing isoprene from cultured cells wherein the cells in the stationary phase. The invention also provides compositions that include these cultured cells and/or increased amount of isoprene. The invention also provides for systems that include a nonflammable concentration of isoprene in the gas phase. Additionally, the invention provides isoprene compositions, such as compositions with increased amount of isoprene or increased purity. 122-. (canceled)23. A method of producing isoprene , the method comprising(a) culturing recombinant cells comprising a heterologous nucleic acid encoding an isoprene synthase polypeptide under suitable culture conditions for the production of isoprene, wherein the amount of isoprene produced during stationary phase is greater than or about 2-fold more than the amount of isoprene produced during growth phase for the same length of time; and(b) producing isoprene.24. The method of claim 23 , wherein the cells are cultured under limited glucose conditions.25. The method of claim 23 , further comprising (c) recovering the isoprene.2631-. (canceled)32. The method of claim 25 , wherein the isoprene is recovered from an off-gas portion of the cell culture.33. The method of claim 23 , wherein the isoprene synthase polypeptide is a plant isoprene synthase polypeptide.34. The method of claim 23 , wherein the cells are bacterial cells.35Escherichia coli, Pantoae citrea, Bacillus subtilis, Bacillus licheniformis, Bacillus lentus, Bacillus brevis, Bacillus stearothermophilus, Bacillus alkalophilus, Bacillus amyloliquefaciens, Bacillus clausii, Bacillus halodurans, Bacillus megaterium, Bacillus coagulans, Bacillus circulans, Bacillus lautus, Bacillus thuringiensis, Streptomyces lividans, Streptomyces coelicolor, Streptomyces griseus, PseudomonasPseudomonas alcaligenes. The method of claim 34 , wherein the bacterial cells are selected from the group consisting of sp. claim 34 , and cells.36. The method of claim ...
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