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Небесная энциклопедия

Космические корабли и станции, автоматические КА и методы их проектирования, бортовые комплексы управления, системы и средства жизнеобеспечения, особенности технологии производства ракетно-космических систем

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Мониторинг СМИ

Мониторинг СМИ и социальных сетей. Сканирование интернета, новостных сайтов, специализированных контентных площадок на базе мессенджеров. Гибкие настройки фильтров и первоначальных источников.

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Применить Всего найдено 7346. Отображено 100.

12-01-2012 дата публикации

Olefin production process

Номер: US20120010453A1

Автор: Kenji Fujiwara, Masayasu Ishibashi, Terunori Fujita, Tsuneyuki Ohkubo

Принадлежит: Mitsui Chemicals Inc

A novel olefin production process is provided which can be established as an industrial and practical process capable of producing olefins by directly reacting a ketone and hydrogen in a single reaction step. In particular, a novel olefin production process is provided in which propylene is obtained with high selectivity by directly reacting acetone and hydrogen. The olefin production process according to the present invention includes reacting a ketone and hydrogen in the presence of at least one dehydration catalyst and a silver-containing catalyst, and the at least one dehydration catalyst is selected from metal oxide catalysts containing a Group 6 element, zeolites, aluminas and heteropoly acid salts in which part or all the protons in heteropoly acids are exchanged with metal cations.

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Номер записи: 1

26-01-2012 дата публикации

Microorganisms and methods for the biosynthesis of aromatics, 2,4-pentadienoate and 1,3-butadiene

Номер: US20120021478A1

Автор: Anthony P. Burgard, Mark J. Burk, Priti Pharkya, Robin E. Osterhout

Принадлежит: Genomatica Inc

The invention provides non-naturally occurring microbial organisms having a toluene, benzene, p-toluate, terephthalate, (2-hydroxy-3-methyl-4-oxobutoxy)phosphonate, (2-hydroxy-4-oxobutoxy)phosphonate, benzoate, styrene, 2,4-pentadienoate, 3-butene-1ol or 1,3-butadiene pathway. The invention additionally provides methods of using such organisms to produce toluene, benzene, p-toluate, terephthalate, (2-hydroxy-3-methyl-4-oxobutoxy)phosphonate, (2-hydroxy-4-oxobutoxy)phosphonate, benzoate, styrene, 2,4-pentadienoate, 3-butene-1ol or 1,3-butadiene.

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Номер записи: 2

16-02-2012 дата публикации

Process for the stereoselective enzymatic reduction of keto compounds

Номер: US20120040425A1

Автор: Anke Tschentscher, Antje Gupta, Maria Bobkova

Принадлежит: IEP GMBH

In a process for the stereoselective, in particular enantioselective enzymatic reduction of keto compounds to the corresponding chiral hydroxy compounds, wherein the keto compounds are reduced with an enantioselective, NADH-specific oxidoreductase, a polypeptide is used for reducing the keto compounds, which polypeptide exhibits an R-ADH-signature H-[P; A]-[I; A; Q; V; L]-[G; K]-R at positions 204-208 and the following further structural features in their entirety: (i) an N-terminal Rossmann-Fold GxxxGxG, (ii) an NAG-motif at position 87, (iii) a catalytic triad consisting of S 139, Y 152 and K 156, (iv) a negatively charged amino acid moiety at position 37, (v) two C-terminal motifs in the dimerization domain [A; S]-S-F and [V; I]-DG-[G; A]-Y-[T; C; L]-[A; T; S]-[Q; V; R; L; P], (vi) Val or Leu at position 159 (4 positions downstream of K 156), (vii) Asn at position 178, and (viii) a proline moiety at position 188.

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Номер записи: 3

15-03-2012 дата публикации

Buffer composition for catalyzing the preparation of calcitriol or calcifediol and method for preparing calcitriol or calcifediol using same

Номер: US20120064584A1

Автор: Dae-Jung Kang, Hyun-Jung Jung, Jae-Hoon Kang, Jong-Hyuk Im

Принадлежит: Ildong Pharmaceutical Co Ltd

The present invention relates to a buffer composition for promoting production of calcitriol or calcifediol, and a method for producing calcitriol or calcifediol using the same. More particularly, the present invention relates to a buffer composition for promoting production of calcitriol or calcifediol comprising a metallic compound, an organic solvent, cyclodextrin, tris(hydroxymethyl)aminomethane, sodium succinate, sodium chloride, magnesium chloride, and water, and a method for producing calcitriol or calcifediol using the same. In the method for producing calcitiriol or calcifediol, the production yield of calcitriol or calcifediol is high, and the bioconversion is carried out in an enzyme reaction system instead of in a microorganism culture system. Thus, it is not required to maintain a sterile state. Also, the separation/purification following the completion of a biocatalytic reaction can be carried out in a cleaner state than the microorganism culture method. Accordingly, there is an advantage in that a cost required for separation is low and the quality is improved. Furthermore, the buffer composition for promoting production of calcitriol or calcifediol can provide a high productivity of calcitriol or calcifediol.

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Номер записи: 4

03-05-2012 дата публикации

Production Of Pure Lignin From Lignocellulosic Biomass

Номер: US20120108798A1

Автор: David A. Hogsett, John Basdsley, Kevin S. Wenger, Michael Ladisch

Принадлежит: Mascoma Corp

The present invention is directed to a process of producing substantially pure lignin from lignocellulosic biomass, which comprises: pre-treating a lignocellulosic feedstock to produce a reactive lignin-carbohydrate mixture; biologically-reacting the carbohydrates in the mixture, separating remaining solids from the liquid fermentation products, and drying the resulting solids to yield a substantially pure lignin product. Optionally, the lignin product may be washed and subjected to a second hydrolysis step. Optionally, the lignin product may be further processed by hydrotreating and/or pyrolysis in order to yield desirable products such as fuel additives.

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Номер записи: 5

31-05-2012 дата публикации

Method for producing microbial fermentation product

Номер: US20120135484A1

Автор: Kazuhiro Onozuka, Saki Hamada, Shingo Koyama, Takaaki Watanabe

Принадлежит: Kao Corp

Disclosed is a method for producing 1-(2-hydroxyethyl) 2,5,5,8a-tetramethyldecahydronaphthalene-2-ol represented by formula (2), wherein microbial conversion is carried out using a compound(s) represented by formula (1a) and/or (1b) as a substrate, the resulting culture product, in which microorganisms obtained by the microbial conversion are contained, and a solvent having an SP value within the range of 7.5 to 9.0 [(cal/cm 3 ) 1/2 ] are mixed together, and subsequently the aqueous phase is removed therefrom.

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Номер записи: 6

06-12-2012 дата публикации

Substrate-selective co-fermentation process

Номер: US20120308991A1

Автор: Elliot Altman, Mark Eiteman

Принадлежит: University of Georgia Research Foundation Inc UGARF

Biological method for conversion of a sugar-containing organic material into a desired biochemical product. Use of a plurality of substrate-selective cells allows different sugars in a complex mixture to be consumed concurrently and independently. The method can be readily extended to remove inhibitory compounds from hydrolysate.

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Номер записи: 7

17-01-2013 дата публикации

Process for the production of alcohols and/or solvents from papermaking pulps with recycling of non-hydrolyzed vegetation

Номер: US20130017585A1

Автор: Caroline Aymard, Marcel Ropars

Принадлежит: IFP Energies Nouvelles IFPEN

This invention describes a process for the production of alcohols and/or solvents from cellulosic or lignocellulosic biomass that comprises at least the following stages: a) Alkaline chemical pretreatment based on sodium sulfate of a cellulosic or lignocellulosic substrate; b) Washing of the pretreated substrate; c) Enzymatic hydrolysis of the substrate that is pretreated and washed using cellulolytic and/or hemicellulolytic enzymes that produce a hydrolyzate and a water-insoluble residue; d) Microorganism fermentation of the hydrolyzate that is obtained from stage c) and production of a fermentation must that contains at least one alcohol and/or solvent; e) Separation/purification of alcohol and/or solvent, and f) Separation of a cake that contains the insoluble residue, in which at least a portion of the cake that is obtained in stage f) is recycled upstream from the pretreatment stage a) and/or upstream from the washing stage b).

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Номер записи: 8

21-03-2013 дата публикации

Process for Producing Aliskiren

Номер: US20130071899A1

Автор: Adele Russo, Antonio Carlo Zanotti-Gerosa, Elena Cini, Luca Banfi, Luca Carcone, Marcello Rasparini, Maurizio Taddei, Renata Riva, Romina Vitale, Stephen Roseblade

Принадлежит: Chemo Iberica SA

A new route of synthesis of the compound Aliskiren of formula (I), used in the treatment of hypertension, is described.

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Номер записи: 9

28-03-2013 дата публикации

Polypeptides having xylanase activity and polynucleotides encoding same

Номер: US20130078672A1

Автор: Hanshu Ding, Junxin Duan, Lan Tang, Ye Liu

Принадлежит: Novozymes AS, Novozymes Inc

The present invention relates to isolated polypeptides having xylanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

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Номер записи: 10

28-03-2013 дата публикации

KETOREDUCTASE POLYPEPTIDES FOR THE REDUCTION OF ACETOPHENONES

Номер: US20130078692A1

Автор: Ching Charlene, Gruber John M., Huisman Gjalt W., Jenne Stephane J., Krebber Anke, Liang Jack, Mundorff Emily

Принадлежит: CODEXIS, INC.

The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds. 1Lactobacillus. A method for stereoselectively reducing a 2′ ,6′-substituted acetophenone substrate , optionally substituted at one or more positions selected from the group consisting of 3′ , 4′ , and 5′ , to the corresponding substituted (S)-1-phenethanol , which comprises contacting the substrate with an engineered ketoreductase polypeptide under reaction conditions suitable for stereoselectively reducing or converting the substrate to the corresponding substituted (S)-1-phenethanol product , wherein the engineered ketoreductase polypeptide is derived from a wild-type ketoreductase and is capable of stereoselectively reducing acetophenone to (S)-1-phenethanol with a percent stereomeric excess of at least about 90%.2. The method of claim 1 , wherein the substrate is 2′ claim 1 ,6′-dichloro-3′-fluoroacetophenone and the corresponding substituted (S)-1-phenethanol product is (S)-1-(2 claim 1 ,6-dichloro-3-fluorophenyl)ethanol.3. The method of claim 1 , wherein the (S)-1-(2 claim 1 ,6-dichloro-3-fluorophenyl)ethanol is formed in greater than 99% stereomeric excess.4. The method of claim 1 , wherein at least about 95% of the substrate is reduced to the product in less than 24 hours when the method is conducted with at least 200 g/L of substrate and with less than 2 g/L of the polypeptide.5. The method of claim 1 , wherein the method is carried out with whole cells that express the engineered ketoreductase polypeptide claim 1 , or an extract or lysate of such cells.6. The method of claim 1 , wherein the engineered ketoreductase polypeptide is isolated and/or ...

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Номер записи: 11

04-04-2013 дата публикации

Transformed cells that ferment-pentose sugars and methods of their use

Номер: US20130084617A1

Автор: Christiaan Van Der Drift, Harry Ramancedj Harhangi, Hubertus Johannes Marie Op Den Camp, Jacobus Thomas Pronk

Принадлежит: C5 Yeast Co BV

The present invention relates to host cells transformed with a nucleic acid sequence encoding a eukaryotic xylose isomerase obtainable from an anaerobic fungus. When expressed, the sequence encoding the xylose isomerase confers to the host cell the ability to convert xylose to xylulose which may be further metabolized by the host cell. Thus, the host cell is capable of growth on xylose as carbon source. The host cell preferably is a eukaryotic microorganism such as a yeast or a filamentous fungus. The invention further relates to processes for the production of fermentation products such as ethanol, in which a host cell of the invention uses xylose for growth and for the production of the fermentation product. The invention further relates to nucleic acid sequences encoding eukaryotic xylose isomerases and xylulose kinases as obtainable from anaerobic fungi.

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Номер записи: 12

02-05-2013 дата публикации

Methods For Producing Acetone, Butanol, and Ethanol

Номер: US20130109068A1

Автор: Ellis Joshua T., Miller Charles, Sathish Ashik, Sims Ronald

Принадлежит: Utah State University

Methods of producing solvents from algae, where the methods include processing algae to yield processed biomass, fermenting the processed biomass with a bacteria to yield solvents. 1. A method of producing solvents from algae , the method comprising:processing algae to yield processed biomass,{'i': 'Clostridium', 'fermenting the processed biomass with a bacteria to yield solvents.'}2. The method of claim 1 , wherein processing algae comprises:hydrolyzing a slurry comprising algae and water by adding an acidic hydrolyzing agent to yield an acidic slurry,hydrolyzing the acidic slurry by adding a basic hydrolyzing agent to yield a basic slurry, andseparating biomass from an aqueous phase to yield processed biomass.3. The method of claim 2 , wherein the slurry has a solid content of about 4-25%.4. The method of claim 2 , wherein the acidic hydrolyzing agent is selected from the group consisting of a strong acid claim 2 , a mineral acid claim 2 , sulfuric acid claim 2 , hydrochloric acid claim 2 , phosphoric acid claim 2 , and nitric acid.5. The method of claim 2 , wherein the acidic slurry has a pH of from about 1.5-4.6. The method of claim 2 , wherein the acidic slurry is heated to a temperature of from about 50-120 ° C.7. The method of claim 2 , wherein the basic hydrolyzing agent is selected from the group consisting of a strong base claim 2 , sodium hydroxide claim 2 , and potassium hydroxide.8. The method of claim 2 , wherein the basic slurry has a pH of from about 8-14.9. The method of claim 2 , wherein the basic slurry is heated to a temperature of from about 50-120 ° C.10. The method of claim 1 , wherein processing algae comprises mechanically shearing the algae.11ClostridiumClostridium saccharoperbutylacetonium, Clostridium acetobutylicumClostridium beijerinckii.. The method of claim 1 , wherein the bacteria are selected from the group consisting of claim 1 , and12. The method of claim 1 , wherein the solvents are selected from the group consisting of butanol ...

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Номер записи: 13

09-05-2013 дата публикации

Amycolatopsis sp. Strain and Methods of Using the Same for Vanillin Production

Номер: US20130115667A1

Автор: CHU Shibiao, Li Hui, WANG Xinglin, ZHENG Pu, ZHOU Jianhai

Принадлежит:

This invention provides an sp. strain (zhp06), and a method of using the whole cell preparation of the strain for vanillin production. The strain was deposited in China Center for Type Culture Collection on Jul. 26, 2011 with the number of CCTCC NO: M 2011265. Under high concentrations of ferulic acid substrate, the vanillin production by this method can reach more than 10 g/L. The molar conversion rate of ferulic acid is more than 50% and the purity of vanillin is from 80% to 95%. The advantage of this invention includes: repeated use of biocatalyst cells, mild biotransformation condition, low environmental pollution, short production cycle, high product purity and simple purification procedure. It has a great potential for industrial applications. 1AmycolatopsisAmycolatopsis. An sp. strain (zhp06) deposited in China Center for Type Culture Collection on Jul. 26 , 2011 with the number of CCTCC NO: M 2011265 , wherein the characteristic of said strain is as follows: the body of said strain is filamentous , the colony color is incanus or yellow , the spore appears ellipsoid , the optimal temperature for growth is from 28° C. to 37° C. with the ability to grow at 50-55° C. , and its 16S rRNA sequence shares 98-99% homology with those of many known sp. strains.2Amycolatopsis. A method for vanillin production using cells of or its mutant strains (cells) claim 1 , comprising the steps of{'i': Amycolatopsis', 'Amycolatopsis, 'a) cultivation of said cells using a standard culture procedure and collection of said cells by centrifugation;'}{'i': Amycolatopsis', 'Amycolatopsis, 'b) addition of said cells obtained by step a) to a substrate solution to form a biotransformation solution, and conversion of ferulic acid to vanillin using said cells as a biocatalyst;'}c) purification of vanillin from said biotransformation solution using a macroporous resin.3. The method according to claim 2 , wherein a fermentation medium containing 5˜15 g/L glucose claim 2 , 1˜20 g/L yeast powder ...

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Номер записи: 14

09-05-2013 дата публикации

PRODUCTION OF ARABITAL

Номер: US20130116475A1

Автор: Ju Lu-Kwang

Принадлежит: THE UNIVERSITY OF AKRON

A method for producing arabitol, and more particularly to producing arabitol in a major amount based on a total weight of all polyols produced and in relatively high concentration from a mixture including a carbon source such as glycerol. The method includes in one embodiment utilizing select yeast strains to produce arabitol in high yield while minimizing the amounts of other polyols, using carbon sources such as glycerol as a component in a medium. In a beneficial embodiment, biodiesel byproduct glycerol is used as the substrate for arabitol production. 1. A process for producing arabitol , comprising the steps of:combining a carbon source with a yeast in a medium, forming a medium mixture; andproducing a major amount of the arabitol based on a total weight of all polyols produced by the process, and wherein the arabitol is produced in a concentration greater than 30 g/L of the medium and in at least 40% conversion of the carbon source consumed.2Debaryomyces, MetschnikowiaGeotrichum.. The process according to claim 1 , wherein the yeast is of one or more of the genera and3DebaryomycesMetschnikowia.. The process according to claim 2 , wherein the yeast is one or more of the genera and4. The process according to claim 2 , wherein the carbon source is one or more of glycerol claim 2 , glucose claim 2 , xylose and a hydrolysate of lignocellulosic biomass.5. The process according to claim 4 , further comprising the step of obtaining the glycerol as a byproduct from biodiesel production.6D. henseniiM. zobellii.. The process according to claim 3 , wherein the yeast is one or more of and7. The process according to claim 4 , wherein at least glycerol is used as the carbon source claim 4 , wherein the concentration of the glycerol in the initial mixture is from about 50 to about 400 g/L of the medium claim 4 , wherein concentration of the yeast in the initial mixture is from about 0.05 to about 5 g/L of the medium claim 4 , wherein the arabitol is produced in an amount ...

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Номер записи: 15

23-05-2013 дата публикации

Production of renewable aromatic compounds

Номер: US20130130345A1

Автор: David A. Sikkenga, Wendy Thai, Will SCHROEDER

Принадлежит: JNF BIOCHEMICALS LLC

The invention provides a process for producing a variety renewable aromatic compounds such as benzene, toluene, xylenes, and cumene, as well as compounds derived from these including, for example, aniline, benzoic acid, cresol, cyclohexane, cyclohexanone, phenol and bisphenol A, toluene di-isocyanate, isophthalic acid, phthalic anhydride, terephthalic acid and dimethyl terephthalate. The invention also provides for renewable forms of these aromatic compounds.

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Номер записи: 16

30-05-2013 дата публикации

System and Method of Producing Volatile Organic Compounds from Fungi

Номер: US20130137131A1

Автор: Angela R. Tomsheck, Gary A. Strobel

Принадлежит: Individual

An isolated fungus is described. The isolated fungus produces at least one compound selected from the group consisting of 1,8-cineole, 1-methyl-1, 4-cyclohexadiene, and (+)-α-methylene-α-fenchocamphorone. A method for producing at least one compound selected from the group consisting of 1,8-cineole, 1-methyl-1, 4-cyclohexadiene, and (+)-α-methylene-α-fenchocamphorone is also described. The method includes culturing a fungus on or within a culturing media in a container under conditions sufficient for producing the at least one compound.

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Номер записи: 17

13-06-2013 дата публикации

Whole-Cell Biotransformation Of Fatty Acids To Obtain Fatty Aldehydes Shortened By One Carbon Atom

Номер: US20130149756A1

Автор: Buchhaupt Markus, Schrader Jens, Sporleder Fenja

Принадлежит: SYMRISE AG

The present invention relates to the area of producing aliphatic aldehydes with 5 to 31 carbon atoms, in particular by microbial conversion of corresponding aliphatic fatty acids with 6 to 32 carbon atoms. The invention also relates to enzymes for catalysing a conversion reaction of this type and nucleic acids coding for this. 1. A method for producing an aldehyde with 5 to 31 carbon atoms comprising:providing microorganism cells containing a dioxygenase;applying a conversion medium containing a fatty acid with 6 to 32 carbon atoms to the microorganism cells; andconverting the fatty acid to the aldehyde by means of the dioxygenase.2. The method according to claim 1 , wherein the dioxygenase is an alpha-dioxygenase.3. The method according to claim 2 , wherein the dioxygenasehas an amino acid sequence similarity to SEQ ID No. 1 of at least 80% measured using the Waterman-Smith algorithm with a gap open penalty of 10, a gap extension penalty of 0.5 and the Blosum62 matrix; andhas an amino acid sequence according to SEQ ID No. 2.4. The method according to claim 2 , wherein the dioxygenase has a peroxidase activity of at most 0.4 nkat/mg guaiacol/2-hydroperoxypalmitic acid.5. The method according to claim 1 , wherein the fatty acid is selected from the group consisting of:linear or branched saturated fatty acids;linear or branched unsaturated fatty acids; andunsaturated or saturated fatty acids with 1 to 5 substituents, wherein the substituents are in each case selected independently from hydroxy, C1-C10-alkyl, C1-C10-alkoxy, C6-C10-aryl, phenyl-C1-C5-alkyl and phenyl-C1-C5-alkenyl, wherein the alpha-C atom of the fatty acid does not carry any such substituent.7. The method according to claim 1 , wherein the microorganism cells are selected from microorganismsselected from the group consisting of the classes of gamma proteobacteria, bacilli, and saccharomycetes.8. The method according to claim 1 , wherein applying a conversion medium containing a fatty acid with 6 to 32 ...

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Номер записи: 18

11-07-2013 дата публикации

RECOMBINANT PROCESS FOR THE PRODUCTION OF R-AROMATIC ALPHA HYDROXY KETONES

Номер: US20130177953A1

Автор: Agrawal Praveen Kumar, Bhat P. Jayadeva, Noronha Santosh

Принадлежит: INDIAN INSTITUTE OF TECHNOLOGY, BOMBAY

A process for preparation of (R)-aromatic α-hydroxy ketones of formula (I), said process occurring in strain(s) of yeast expressing a recombinant pyruvate decarboxylase, having cysteine residues at positions 221 and 222 of PDC1, an isoenzyme of said pyruvate decarboxylase, substituted with glutamate and alanine respectively such that the said mutation being in the regulatory site of pyruvate decarboxylase, selectively favours carboligation reaction over decarboxylation. 2. The process as claimed in wherein the improvement for PDC production is in the level of about 57 mM (˜61% biotransformation efficiency w.r.t benzaldehyde) along with feedstock conservation as a consequence of lower pyruvate wastage.3. The process as claimed in wherein the cysteine residues are at position 221 and 222 of PDC1.4. The process as claimed in wherein the enzyme is being expressed through a plasmid or integrated genomically.5. The process as claimed in wherein R is H or CH;{'sub': 1-3', '1-3', '2', '1', '2', '1', '2', '1-4', '1-3, 'wherein R′ is phenyl or optionally substituted phenyl wherein the substituents are selected from the group Calkyl, Calkoxy, F, CI, Br, I, OH, NH, CN and NRR, wherein Rand Rcan be independently H or (C) alkyl and the said Calkyl can be further substituted by a substituent chosen from F, CI, Br, I and OH and also pyridine, thiophene, furan, napthaldehyde and their substituents.'}6. The process as claimed in comprising isolating a polypeptide encoded by the nucleic acid sequence of PDC1 from yeasts and substituting the amino acid at the substrate regulatory site at positions 221 and 222.7. The process as claimed in where the strain(s) of yeast expressing a polypeptide having pyruvate decarboxylase activity claim 1 , comprising a polypeptide encoded by the nucleic acid sequence of PDC5.8. The process as claimed in the where the cysteine at 221 is replaced with glutamic acid in PDC5.9. The process as claimed in where the strain(s) of yeast having a polypeptide that ...

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Номер записи: 19

25-07-2013 дата публикации

MICROORGANISMS FOR THE PRODUCTION OF 1,4-BUTANEDIOL, 4-HYDROXYBUTANAL, 4-HYDROXYBUTYRYL-COA, PUTRESCINE AND RELATED COMPOUNDS, AND METHODS RELATED THERETO

Номер: US20130189751A1

Автор: Burgard Anthony P., Haselbeck Robert, NIU Wei, Trawick John D.

Принадлежит: GENOMATICA, INC.

The invention provides non-naturally occurring microbial organisms comprising a 1,4-butanediol (BDO), 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine pathway comprising at least one exogenous nucleic acid encoding a BDO, 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine pathway enzyme expressed in a sufficient amount to produce BDO, 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine and further optimized for expression of BDO. The invention additionally provides methods of using such microbial organisms to produce BDO, 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine. 1. A non-naturally occurring microbial organism , comprising a 4-hydroxybutanal pathway comprising at least one exogenous nucleic acid encoding a 4-hydroxybutanal pathway enzyme expressed in a sufficient amount to produce 4-hydroxybutanal , said 4-hydroxybutanal pathway comprising:succinyl-CoA reductase (aldehyde forming); 4-hydroxybutyrate dehydrogenase; and 4-hydroxybutyrate reductase;alpha-ketoglutarate decarboxylase; 4-hydroxybutyrate dehydrogenase; and 4-hydroxybutyrate reductase;succinate reductase; 4-hydroxybutyrate dehydrogenase, and 4-hydroxybutyrate reductase;alpha-ketoglutarate decarboxylase, or glutamate dehydrogenase or glutamate transaminase and glutamate decarboxylase and 4-aminobutyrate dehydrogenase or 4-aminobutyrate transaminase; 4-hydroxybutyrate dehydrogenase; and 4-hydroxybutyrate reductase; oralpha-ketoglutarate reductase; 5-hydroxy-2-oxopentanoate dehydrogenase; and 5-hydroxy-2-oxopentanoate decarboxylase.2. The non-naturally occurring microbial organism of claim 1 , wherein said microbial organism comprises three exogenous nucleic acids encoding succinyl-CoA reductase (aldehyde forming); 4-hydroxybutyrate dehydrogenase; and 4-hydroxybutyrate reductase3. The non-naturally occurring microbial organism of claim 1 , wherein said at least one exogenous nucleic acid is a heterologous nucleic acid.4. A method for producing 4-hydroxybutanal claim 1 , comprising culturing ...

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Номер записи: 20

08-08-2013 дата публикации

KETO-ISOVALERATE DECARBOXYLASE ENZYMES AND METHODS OF USE THEREOF

Номер: US20130203138A1

Автор: HE HONGXIAN, MCELVAIN JESSICA, OKEEFE DANIEL P., Paul Brian James, PAYNE MARK S., ROTHMAN STEVEN CARY

Принадлежит: BUTAMAX(TM) ADVANCED BIOFUELS LLC

Provided herein are polypeptides and polynucleotides encoding such polypeptides which have ketoisovalerate decarboxylase activity. Also provided are recombinant host cells comprising such polypeptides and polynucleotides and methods of use thereof. 1. A method of converting α-ketoisovalerate to isobutyraldehyde comprising (i) at least 80% identity to SEQ ID NO: 51, 52, 53, 55, 56, 58, 59, 61 or 63 or an active fragment thereof; or', {'sub': 'c', '(ii) α-ketoisovalerate decarboxylase activity, a specificity ratio for α-ketoisovalerate to pyruvate greater than 1, and thiamine diphosphate cofactor activation constant (K) of about 20 μM or less; and'}], 'a. providing a polypeptide wherein said polypeptide comprises at least one ofb. contacting said polypeptide with α-ketoisovalerate under conditions wherein isobutyraldehyde is produced.2. The method of wherein the polypeptide has a KIVD cluster profile HMM E value of less than 1E-223 using the hmmsearch program.3. The method of wherein the contacting occurs within a recombinant host cell and wherein the polypeptide is heterologous to recombinant host cell.4Clostridium, Zymomonas, Escherichia, Salmonella, Serratia, Erwinia, Klebsiella, Shigella, Rhodococcus, Pseudomonas, Bacillus, Lactobacillus, Enterococcus, Alcaligenes, Klebsiella, Paenibacillus, Arthrobacter, Corynebacterium, Brevibacterium, Schizosaccharomyces, Issatchenkia, Kluyveromyces, Yarrowia, Pichia, Candida, HansenulaSaccharomyces.. The method of wherein the recombinant host cell is a member of the genera claim 3 , or5Saccharomyces cerevisiae.. The method of wherein the recombinant host cell is6. The method of wherein the recombinant host cell further comprises heterologous polynucleotides encoding polypeptides which catalyze the substrate to product conversions: (a) pyruvate to acetolactate; (b) acetolactate to 2 claim 3 ,3-dihydroxyisovalerate; and (c) 2 claim 3 ,3-dihydroxyisovalerate to 2-ketoisovalerate.7. The method of wherein the host cell further ...

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Номер записи: 21

08-08-2013 дата публикации

CORYNEFORM BACTERIUM TRANSFORMANT AND PROCESS FOR PRODUCING PHENOL USING THE SAME

Номер: US20130203139A1

Автор: INUI MASAYUKI, Yukawa Hideaki

Принадлежит: GREEN PHENOL TECHNOLOGY RESEARCH ASSOCIATION

A phenol-producing transformant constructed by transferring a gene which encodes an enzyme having tyrosine phenol-lyase activity into as a host can efficiently produce phenol from a saccharide. Specifically, preferred is a process which comprises a step of reacting the transformant in a reaction mixture containing a saccharide under reducing conditions, and a step of collecting phenol from the reaction mixture. 1. A phenol-producing transformant constructed by transferring a gene which encodes an enzyme having tyrosine phenol-lyase activity into a coryneform bacterium as a host.2Pantoea agglomeransCitrobacter braakiiDesulfitobacterium hafnienseChloroflexus aurantiacusNostoc punctiformeTreponema denticola.. The transformant of claim 1 , wherein the gene which encodes an enzyme having tyrosine phenol-lyase activity is a gene derived from claim 1 , a gene derived from claim 1 , a gene derived from claim 1 , a gene derived from claim 1 , a gene derived from claim 1 , or a gene derived from3. The transformant of claim 1 , wherein the gene which encodes an enzyme having tyrosine phenol-lyase activity is the DNA of the following (a) or (b).(a) a DNA consisting of the base sequence of SEQ ID NO: 36, a DNA consisting of the base sequence of SEQ ID NO: 39, a DNA consisting of the base sequence of SEQ ID NO: 42, a DNA consisting of the base sequence of SEQ ID NO: 45, a DNA consisting of the base sequence of SEQ ID NO: 48, or a DNA consisting of the base sequence of SEQ ID NO:(b) a DNA which hybridizes to a DNA consisting of a complementary base sequence of any of the DNAs of (a) under stringent conditions and which encodes a polypeptide having tyrosine phenol-lyase activity4. The transformant of claim 1 , wherein the following gene (c) and/or gene (d) on the chromosome of the coryneform bacterium as the host has a disruption or deletion.(c) a gene which encodes an enzyme having prephenate dehydratase activity(d) a gene which encodes an enzyme having phenol 2-monooxygenase ...

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Номер записи: 22

15-08-2013 дата публикации

Method and an Apparatus for Producing Organic Solvents and Alcohols by Microbes

Номер: US20130211143A1

Автор: Granstrom Tom

Принадлежит: AALTO UNIVERSITY FOUNDATION

The present invention relates to a cell retaining matrix (CRB) for producing solvents and alcohols and an apparatus comprising said bio-column. The invention also relates to the method for producing solvents and alcohols by using said cell retaining matrix (CRB). Said cell retaining biomatrix (CRB cellulosic fibers, selected from the group consisting wood cellulosic fibers, pulp cellulosic fibers, vegetable cellulosic fibers, such as mechanical pulp, dissolving pulp, lignocellulosic fibers, and cellulosic fibers originated from vegetable peels, and at microbes, which have been immobilized into said cellulosic fibers, and which can save their biological activity in cell retaining biomatrix (CRB). 1. A cell retaining biomatrix (CRB) in the form of sheet , mat or strip for producing organic solvents and alcohols by microbe(s) from different substrates , wherein the cell retaining biomatrix (CRB) comprises:cellulosic fibers, selected from the group consisting of wood cellulosic fibers, pulp cellulosic fibers, vegetable cellulosic fibers and cellulosic fibers originated from vegetable peels,microbes, which have been immobilized into said cellulosic fibers, and which can save their biological activity in the cell retaining biomatrix (CRB); anda support structure or an effluent or both a support structure and an effluent.2. The cell retaining biomatrix (CRB) according to claim 1 , wherein said biologically active microbes can save their biological activity at least for 14 days in the cell retaining biomatrix (CRB).3. (canceled)4. (canceled)5ClostridiaLactobacillus. The cell retaining biomatrix (CRB) according to claim 1 , wherein said microbe is selected from the group consisting of species claim 1 , and species.6. A bio-column (BC) comprising a cell retaining biomatrix (CRB) according to .7. An apparatus for producing organic solvents and alcohols from different substrates by microbe(s) claim 6 , comprising at least the bio-column (BC) according to .8. The apparatus ...

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Номер записи: 23

15-08-2013 дата публикации

ISOPROPYL ALCOHOL-PRODUCING BACTERIUM HAVING IMPROVED PRODUCTIVITY BY GNTR DESTRUCTION

Номер: US20130211170A1

Автор: AMANO Koh, Furusawa Chikara, Hirano Junichiro, Hirasawa Takashi, Matsumoto Yoshiko, SHIMIZU Hiroshi, Shirai Tomokazu, Takahashi Hitoshi, Takebayashi Nozomi, Wada Mitsufumi

Принадлежит:

An isopropyl alcohol-producing includes an isopropyl alcohol production system, wherein an activity of transcriptional repressor GntR is inactivated, and the isopropyl alcohol-producing preferably further includes a group of auxiliary enzymes having an enzyme activity expression pattern with which isopropyl alcohol production capacity achieved by the inactivation of the GntR activity is maintained or enhanced. A method of producing isopropyl alcohol includes producing isopropyl alcohol from a plant-derived raw material using the isopropyl alcohol-producing . A method of producing acetone includes contacting the isopropyl alcohol obtained by the isopropyl alcohol production method with a complex oxide that includes zinc oxide and at least one oxide containing a Group 4 element, and that is prepared by coprecipitation. A method of producing propylene includes contacting isopropyl alcohol and acetone obtained by the production method with a solid acidic substance and a Cu-containing hydrogenation catalyst as catalysts.

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Номер записи: 24

29-08-2013 дата публикации

Process for the manufacture of butanol or acetone

Номер: US20130219776A1

Автор: Jane Samantha White, Kenneth Alexander Leiper, Martin Tangney, Sandra Messenger

Принадлежит: CELTIC RENEWABLES Ltd

A process for the manufacture of butanol, acetone and/or other renewable chemicals is provided wherein the process utilises one or more of the group comprising by-products of the manufacture of malt whisky, such as draff, pot ale and/or spent lees, biomass substrates, such as paper, sludge from paper manufacture and spent grains from distillers and brewers, and diluents, such as water and spent liquid from other fermentations. The process comprises treating a substrate to hydrolyse it and fermenting the treated substrate at an initial pH in the range of 5.0 to 6.0. Also provided is a biofuel comprising butanol manufactured according to the process of the invention.

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Номер записи: 25

29-08-2013 дата публикации

Recombinant microorganisms and uses therefor

Номер: US20130224838A1

Автор: Fungmin Liew, Michael Koepke, Sean Simpson, Wendy Chen

Принадлежит: Individual

The invention provides, inter alia, methods for the production of acetone, isopropanol and/or precursors of acetone and/or isopropanol by microbial fermentation of substrates comprising CO, genetically modified microorganisms of use in such methods, nucleic acids suitable for preparation of genetically modified microorganisms, a novel alcohol dehydrogenase and nucleic acids encoding same.

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Номер записи: 26

12-09-2013 дата публикации

Integrated Biorefinery

Номер: US20130236941A1

Автор: Burns-Guydish Stacy M., Cann Anthony F., Dalal Dhruti, Fry Lawrence W., Hershkowitz Michael S., McDonald William F., Meyer Andrew D., Nava-Salgado Victor O., Olson Brandon T., Sonico Ishmael M., Walther David C., Zhu Yongming

Принадлежит: COBALT TECHNOLOGIES INC.

Systems and methods for producing bioproducts are shown. The systems and methods herein can be configured and used in an integrated biorefinery. The integrated biorefinery may comprise a sugar production facility such as a sugar mill, a production facility for one or more bioproduct(s) such as butanol, and optionally an ethanol production facility employing the system and method. 1. An integrated biorefinery , comprising:(a) a sugar production facility, wherein sugar-containing biomass is processed to extract sugar, thereby producing a liquid sugar-containing extract and residual bagasse; and(b) a bioproduct production facility, comprising a microorganism that is capable of producing at least one bioproduct in a microbial fermentation process,wherein sugar molecules that are extracted from sugar-containing biomass that is processed in the sugar production facility are provided to the microorganism in a growth medium,wherein the sugar molecules are fermented by the microorganism, thereby producing a fermentation broth that comprises said at least one bioproduct.2. An integrated biorefinery according to claim 1 , wherein the sugar molecules that are provided to the microorganism comprise: (i) at least a portion of the liquid sugar-containing extract claim 1 , and (ii) sugar molecules extracted from at least a portion of the residual bagasse and/or sugar molecules extracted from at least a portion of biomass that is removed from the sugar-containing biomass prior to processing in the sugar production facility.3. An integrated biorefinery according to claim 1 , wherein said sugar-containing biomass comprises sugar cane.4. An integrated biorefinery according to claim 1 , wherein said sugar-production facility comprises a sugar mill.5. An integrated biorefinery according to claim 2 , wherein said sugar-containing biomass comprises sugar cane claim 2 , and wherein said biomass that is removed prior to processing in the sugar production facility comprises cane straw.6. An ...

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Номер записи: 27

19-09-2013 дата публикации

HALOGENATION ENZYMES

Номер: US20130244296A1

Автор: Zhan Jixun

Принадлежит:

The present disclosure relates to an isolated or purified nucleotide sequence comprising a cDNA sequence of an rdc2 at least 60%, 70%, 90%, 95%, 98%, or 100% identical to SEQ ID NO. 1. In a second embodiment, the invention provides for a flavin-dependent halogenase comprising an amino acid sequence of an Rdc2 halogenase at least 60%, 70%, 90%, 95%, 98%, or 100% identical to SEQ ID NO. 2. In related embodiments there are provided herein methods for halogenating compounds by using an Rdc2 halogenase of the present invention. 1. An isolated enzyme comprising an amino acid sequence selected from a group consisting of (i) the amino acid sequence set forth in SEQ ID NO. 2; (ii) an amino acid sequence that has at least 95% identity to the amino acid sequence set forth in SEQ ID NO. 2; or (iii) a functional subunit of the amino acid sequence set forth in SEQ ID NO. 2.2. An isolated enzyme of claim 1 , further comprising an enzyme having halogenase activity.3. An isolated enzyme of claim 1 , further comprising a flavin-dependent halogenase.4. An isolated enzyme of claim 1 , further comprising an enzyme encoded by the nucleic acid molecule sequence set forth in SEQ ID NO: 1.5. An isolated nucleic acid molecule comprising a sequence that encodes a halogenase enzyme having an amino acid sequence selected from a group consisting of (i) the sequence set forth in SEQ ID NO. 2; or (ii) a sequence with at least 95% identity to SEQ ID NO. 2.6. An isolated nucleic acid molecule of claim 5 , further comprising a nucleic acid molecule that encodes an enzyme having halogenase activity.7. An isolated nucleic acid molecule of claim 5 , further comprising a nucleic acid molecule encoding a flavin-dependent halogenase.8. An isolated nucleic acid molecule of claim 5 , further comprising a nucleic acid sequence selected from a group consisting of nucleic acid sequences (i) at least 90% identical claim 5 , (ii) at least 95% identical claim 5 , and (iii) 100% identical to SEQ ID NO: 1.9. An ...

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Номер записи: 28

26-09-2013 дата публикации

ENANTIOSELECTIVE REDUCTION

Номер: US20130252288A1

Автор: De Vries Andreas Hendrikus Maria, De Wildeman Stefaan Marie André, Straatman Henricus Martinus Maria, Vermote Linda M., Verzijl Gerardus Karel Maria

Принадлежит:

The invention relates to a process for the preparation of a chiral compound, comprising enantioselectively reducing a carbon-carbon double bond of an α,β-unsaturated compound in a mixture comprising both an E isomer and a Z isomer of the α,β-unsaturated compound, wherein both E isomer and Z isomer are converted in the presence of a hydrogenation catalyst. 1. Process for the preparation of a chiral compound , comprising enantioselectively reducing a carbon-carbon double bond of an α ,β-unsaturated compound in a mixture comprising both an E isomer and a Z isomer of the α ,β-unsaturated compound , wherein both E isomer and Z isomer are converted in the presence of a catalyst , and wherein the reduction is carried out under isomerising conditions.2. Process according claim 1 , wherein the molar ratio Z isomer to E isomer in the mixture at the start of the process is in the range of 5:95 to 95:5 claim 1 , in particular 10:90 to 90:10 claim 1 , more in particular 20:80 to 80:20.3. Process according to claim 1 , wherein the chiral compound is formed with an enantiomeric excess of at least 50% claim 1 , in particular of at least 80% claim 1 , more in particular of at least 90%.4. Process according to claim 1 , wherein the catalyst is a biocatalyst claim 1 , in particular an enzyme which enzyme may be present in an organism or isolated from an organism claim 1 , and which enzyme preferably is an oxidoreductase claim 1 , more preferably an ene reductase.5. Process according to claim 4 , wherein the biocatalyst is an enzyme and reduction is carried out in the presence of a cofactor regeneration system for the enzyme.6. Process according to claim 4 , wherein the enzyme is a substrate unspecific enzyme.7. Process according to claim 4 , wherein the enzyme is a substrate specific enzyme.8. Process according to claim 4 , wherein the enzyme is selected from the group of ene reductases HYE1 claim 4 , HYE2 claim 4 , P1 and LTB4DH.9. Process according to claim 1 , wherein the ...

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Номер записи: 29

26-09-2013 дата публикации

PRODUCTION OF INDUSTRIALLY RELEVANT COMPOUNDS IN PROKARYOTIC ORGANISMS

Номер: US20130252294A1

Автор: Fox David Thomas Shaw, Hotta Kinya, Koppisch Andrew Thomas, Welsh John D.

Принадлежит:

Disclosed herein are methods for producing compounds (such as 3,4-dihydroxybenzoate, catechol, cis,cis-muconate, or β-carboxy-cis,cis-muconic acid) utilizing biosynthetic pathways in prokaryotic organisms expressing one or more heterologous genes. In some embodiments, the method includes expressing a heterologous asbF gene (for example, a gene having dehydroshikimate dehydratase activity) in a prokaryotic cell under conditions sufficient to produce the one or more compounds and purifying the compound. In additional embodiments, the method further includes expressing one or more of a heterologous 3,4-DHB decarboxylase gene, a heterologous catechol 1,2-dioxygenase gene, and a heterologous 3,4-DHB dioxygenase gene in the prokaryotic cell and purifying the compound. 1. A method for producing a compound utilizing dehydroshikimate as a precursor , wherein the compound is selected from 3 ,4-dihydroxybenzoate (3 ,4-DHB) , catechol , cis ,cis-muconate , and β-carboxy-cis ,cis-muconic acid , comprising:expressing a heterologous gene encoding a protein having dehydroshikimate dehydratase (DHSase) activity in a prokaryotic cell that is a phototroph under conditions sufficient to produce the compound; andpurifying the compound.2. The method of claim 1 , wherein the phototroph is a cyanobacterium.3SynechocystisSynechocystisSynechococcusSpirulinaAnabaena variabilis.. The method of claim 2 , wherein the cyanobacterium is PCC6803 claim 2 , PCC9714 claim 2 , sp. claim 2 , sp. claim 2 , or4Bacillus thuringiensis, Bacillus cereusBacillus anthracis.. The method of claim 1 , wherein the heterologous gene encoding a protein having dehydroshikimate dehydratase (DHSase) activity comprises a gene from claim 1 , or5. The method of claim 1 , wherein the heterologous gene encoding a protein having dehydroshikimate dehydratase (DHSase) activity comprises the nucleic acid sequence set forth as SEQ ID NO: 1 or SEQ ID NO: 3.6. The method of claim 1 , wherein the heterologous gene encoding a protein ...

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Номер записи: 30

10-10-2013 дата публикации

CORYNEFORM BACTERIUM TRANSFORMANT AND PROCESS FOR PRODUCING PHENOL USING THE SAME

Номер: US20130266999A1

Автор: INUI MASAYUKI, Yukawa Hideaki

Принадлежит:

Provided is a phenol-producing transformant constructed by transferring a gene which encodes an enzyme having 4-hydroxybenzoate decarboxylase activity into as a host. Also provided is a process for producing phenol, which comprises a step of allowing the transformant to react in a reaction mixture containing 4-hydroxybenzoate or a salt thereof under reducing conditions, and a step of collecting phenol from the reaction mixture. 1Corynebacterium glutamicum. A phenol-producing transformant constructed by transferring a gene which encodes an enzyme having 4-hydroxybenzoate decarboxylase activity into as a host.2Bacillus subtilis, Bacillus atrophaeus, Bacillus subtilisCitrobacter koseri, Enterobacter aerogenes, Enterobacter cloacae, Enterobacter hormaechei, Enterobacter sakazakii, Escherichia coli, Escherichia fergusonii, Paenibacillus polymyxaPantoea ananatis.. The transformant of claim 1 , wherein the gene which encodes an enzyme having 4-hydroxybenzoate decarboxylase activity is a gene derived from subsp. spizizenii claim 1 , claim 1 , or3. The transformant of claim 1 , wherein the gene which encodes an enzyme having 4-hydroxybenzoate decarboxylase activity is the DNA of the following (a) or (b).(a) a DNA consisting of the base sequence of SEQ ID NO: 16, SEQ ID NO: 23, SEQ ID NO: 26, SEQ ID NO: 29, SEQ ID NO: 32, SEQ ID NO: 35, SEQ ID NO: 38, SEQ ID NO: 41, SEQ ID NO: 44, NO: 47, SEQ ID NO: 50, or SEQ ID NO: 53(b) a DNA which hybridizes to a DNA consisting of a complementary base sequence of any of the DNAs of (a) under stringent conditions and which encodes a polypeptide having 4-hydroxybenzoate decarboxylase activity.4Corynebacterium glutamicumCorynebacterium glutamicum. The transformant of claim 1 , wherein the as the host is a strain of in which a gene which encodes an enzyme having phenol 2-monooxygenase activity on the chromosome is disrupted or deleted.5Corynebacterium glutamicumCorynebacterium glutamicum. The transformant of claim 1 , wherein the as the host ...

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Номер записи: 31

10-10-2013 дата публикации

Coryneform bacterium transformant and process for producing phenol using the same

Номер: US20130267000A1

Автор: Hideaki Yukawa, Masayuki Inui

Принадлежит: Green Phenol Tech Res Association

Provided is a phenol-producing transformant constructed by transferring a gene which encodes an enzyme having tyrosine phenol-lyase activity into a coryneform bacterium as a host. Also provided is a process for producing phenol, which comprises a step of allowing the transformant to react in a reaction mixture containing tyrosine, a salt thereof, or an ester thereof under reducing conditions, and a step of collecting phenol from the reaction mixture.

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Номер записи: 32

10-10-2013 дата публикации

Novel phytochemicals from extracts of maple syrups and maple trees and uses thereof

Номер: US20130267474A1

Автор: Genevieve Beland, Julie Barbeau, Liya Li, Navindra P. Seeram

Принадлежит: FEDERATION DES PRODUCTEURS ACERICOLES DU QUEBEC, Rhode Island University

The present invention describes phytochemicals present in maple syrup and maple tree extracts by butanol, ethyl acetate and methanol. Novel compounds are isolated from maple syrups, including one compound Quebecol generated in the maple syrup manufacturing process. Also described are digesting extract of maple syrup. The phytochemicals may be used for the treatment or prevention of cancers, metabolic syndromes, diabetes, microorganism infections and/or antioxidants.

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Номер записи: 33

17-10-2013 дата публикации

Flavour modulation by bio-processing using flavour forming bacteria strains

Номер: US20130273201A1

Автор: Marcel Braun

Принадлежит: Nestec SA

A fermentation of a milk source with Lactococcus lactis subsp. lactis biovar diacetylactis (CNCM No. I-1962) to form a fermented milk product. The fermented milk product has a flavour and aroma. The fermented milk product can be in the form of a powder or a concentrate. The fermented milk product has applications in the food industry.

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Номер записи: 34

17-10-2013 дата публикации

Detoxification of Biomass Derived Acetate Via Metabolic Conversion to Ethanol, Acetone, Isopropanol, or Ethyl Acetate

Номер: US20130273555A1

Автор: Argyros Aaron, Froehlich Allan C., Gilbert Alan Benjamin, Hogsett David A., IV Arthur J., Licht Steve, Mcbride John E., Rajgarhia Vineet B., Shaw, Sillers William Ryan, Van Dijken Hans, Xu Haowen

Принадлежит: Mascoma Corporation

One aspect of the invention relates to a genetically modified thermophilic or mesophilic microorganism, wherein a first native gene is partially, substantially, or completely deleted, silenced, inactivated, or down-regulated, which first native gene encodes a first native enzyme involved in the metabolic production of an organic acid or a salt thereof, thereby increasing the native ability of said thermophilic or mesophilic microorganism to produce lactate or acetate as a fermentation product. In certain embodiments, the aforementioned microorganism further comprises a first non-native gene, which first non-native gene encodes a first non-native enzyme involved in the metabolic production of lactate or acetate. Another aspect of the invention relates to a process for converting lignocellulosic biomass to lactate or acetate, comprising contacting lignocellulosic biomass with a genetically modified thermophilic or mesophilic microorganism. 1. A recombinant microorganism comprising one or more native and/or heterologous enzymes that function in one or more engineered metabolic pathways to convert acetate to ethanol , wherein said one or more native and/or heterologous enzymes is activated , upregulated or down-regulated.2. (canceled)3. The recombinant microorganism of claim 1 , wherein one of said engineered metabolic pathways comprises the following steps: (a) conversion of acetate to acetyl-CoA and (b) conversion of acetyl-CoA to ethanol.4. The recombinant microorganism of claim 1 , wherein said one or more downregulated native enzymes is encoded by a gpd1 polynucleotide claim 1 , a gpd2 polynucleotide claim 1 , or both a gpd1 polynucleotide and a gpd2 polynucleotide.5. (canceled)6Saccharomyces cerevisiae, Kluyveromyces lactis, Kluyveromyces marxianus, Pichia pastoris, Yarrowia lipolytica, Hansenula polymorpha, Phaffia rhodozyma, Candida utliis, Arxula adeninivorans, Pichia stipitis, Debaryomyces hansenii, Debaryomyces polymorphus, Schizosaccharomyces pombe, Candida ...

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Номер записи: 35

17-10-2013 дата публикации

Methods for the Economical Production of Biofuel from Biomass

Номер: US20130273622A1

Автор: Aristidou Aristos A., Buelter Thomas, Evanko William A., Glassner David A., Gruber Patrick R., Hawkins Andrew C., Landwehr Marco, Meinhold Peter, Peters Matthew W., Wade James L.

Принадлежит:

Methods for producing a biofuel are provided. Also provided are biocatalysts that convert a feedstock to a biofuel. 1. A method for producing distillers dried grains derived from a fermentation process for the production of isobutanol , said method comprising:(a) cultivating a yeast biocatalyst in a fermentation medium comprising at least one carbon source, wherein said yeast biocatalyst is engineered to express exogenous genes encoding an acetolactate synthase, a ketol-acid reductoisomerase, a dihydroxy acid dehydratase, a ketoisovalerate decarboxylase, and an isobutyraldehyde dehydrogenase;(b) harvesting insoluble material derived from the fermentation process, said insoluble material comprising said yeast biocatalyst; and(c) drying said insoluble material comprising said yeast biocatalyst to produce the distillers dried grains.2. The method of claim 1 , wherein said method further comprises step (d) of adding soluble residual material from the fermentation process to said distillers dried grains to produce distillers dried grains and solubles.3. The method of claim 1 , wherein said distillers dried grains comprise at least one additional product selected from the group consisting of unconsumed feedstock solids claim 1 , nutrients claim 1 , proteins claim 1 , fibers claim 1 , and oils.4. The method of claim 1 , wherein said acetolactate synthase catalyzes the conversion of pyruvate to acetolactate.5. The method of claim 1 , wherein said ketol-acid reductoisomerase catalyzes the conversion of acetolactate to 2 claim 1 ,3-dihydroxyisovalerate.6. The method of claim 1 , wherein said dihydroxy acid dehydratase catalyzes the conversion of 2 claim 1 ,3-dihydroxyisovalerate to a-ketoisovalerate.7. The method of claim 1 , wherein said ketoisovalerate decarboxylase catalyzes the conversion of a-ketoisovalerate to isobutyraldehyde.8. The method of claim 1 , wherein said isobutyraldehyde dehydrogenase catalyzes the conversion of isobutyraldehyde to isobutanol.9. The method ...

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Номер записи: 36

17-10-2013 дата публикации

CORYNEFORM BACTERIUM TRANSFORMANT AND PROCESS FOR PRODUCING PHENOL USING THE SAME

Номер: US20130273624A1

Автор: INUI MASAYUKI, Yukawa Hideaki

Принадлежит: GREEN PHENOL TECHNOLOGY RESEARCH ASSOCIATION

Provided is a phenol-producing transformant constructed by transferring a gene which encodes an enzyme having chorismate-pyruvate lyase activity and a gene which encodes an enzyme having 4-hydroxybenzoate decarboxylase activity into a coryneform bacterium as a host. Also provided is a process for producing phenol, which comprises a step of allowing the transformant to react in a reaction mixture containing a saccharide under reducing conditions, and a step of collecting phenol from the reaction mixture. 1. A phenol-producing transformant constructed by transferring a gene which encodes an enzyme having chorismate-pyruvate lyase activity and a gene which encodes an enzyme having 4-hydroxybenzoate decarboxylase activity into a coryneform bacterium as a host.2Escherichia coliPseudomonas putidaAcinetobacter baumanniiAzotobacter vinelandiiChromohalobacter salexigensCitrobacterCitrobacter koseriCitrobacter youngaeEnterobacter cloacaeMarinobacter aquaeoleiMarinomonas mediterraneaPantoea ananatisPseudoalteromonas haloplanktisRalstonia eutrophaShewanella putrefaciensThiobacillus denitrificans.. The transformant of claim 1 , wherein the gene which encodes an enzyme having chorismate-pyruvate lyase activity is a gene derived from ; a gene derived from ; a gene derived from ; a gene derived from ; a gene derived from ; a gene derived from members of the genus claim 1 , such as and ; a gene derived from ; a gene derived from ; a gene derived from ; a gene derived from ; a gene derived from ; a gene derived from ; a gene derived from ; or a gene derived from3Bacillus subtilisBacillus atrophaeusBacillus subtilisspizizeniiCitrobacter koseriEnterobacter aerogenesEnterobacter cloacaeEnterobacter hormaecheiEnterobacter sakazakiiEscherichia coliEscherichia fergusoniiPaenibacillus polymyxaPantoea ananatis.. The transformant of claim 1 , wherein the gene which encodes an enzyme having 4-hydroxybenzoate decarboxylase activity is a gene derived from claim 1 , a gene derived from claim 1 , ...

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Номер записи: 37

07-11-2013 дата публикации

Processing biomass

Номер: US20130295624A1

Автор: Marshall Medoff, Thomas Craig Masterman

Принадлежит: Xyleco Inc

Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful products, such as fuels. For example, systems can use feedstock materials, such as cellulosic and/or lignocellulosic materials, to produce ethanol and/or butanol, e.g., by fermentation.

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Номер записи: 38

14-11-2013 дата публикации

Process for the manufacture of butanol or acetone

Номер: US20130298453A1

Автор: Jane Samantha White, Kenneth Alexander Leiper, Martin Tangney, Sandra Messenger

Принадлежит: CELTIC RENEWABLES Ltd

A process for the manufacture of butanol, acetone and/or other renewable chemicals is provided wherein the process utilises one or more of the group comprising by-products of the manufacture of malt whisky, such as pot ale and/or spent lees, biomass substrates, such as paper, sludge from paper manufacture and spent grains from distillers and brewers, and diluents, such as water and spent liquid from other fermentations. The process comprises treating a substrate to hydrolyse it and fermenting the treated. Also provided is a biofuel comprising butanol manufactured according to the process of the invention.

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Номер записи: 39

21-11-2013 дата публикации

Methods for Degrading or Converting Cellulosic Material

Номер: US20130309723A1

Автор: Huang Hongzhi, Ren Haiyu

Принадлежит: NOVZYMES A/S

Provided are methods for degrading or converting a cellulosic material, comprising: treating the cellulosic material with an enzyme composition in the presence of a polypeptide having catalase activity; and enzyme composition used for degrading or converting a cellulosic material comprising one or more (e.g., several) enzymes having cellulolytic and/or hemicellulolytic activity and a polypeptide having catalase activity. 125-. (canceled)26. A method for degrading or converting a cellulosic material , comprising: treating the cellulosic material with an enzyme composition in the presence of a polypeptide having catalase activity.27. The method of claim 26 , wherein the enzyme composition comprises one or more enzymes selected from the group consisting of a cellulase claim 26 , a GH61 polypeptide having cellulolytic enhancing activity claim 26 , a hemicellulase claim 26 , an esterase claim 26 , an expansin claim 26 , a laccase claim 26 , a ligninolytic enzyme claim 26 , a pectinase claim 26 , a peroxidase claim 26 , a protease claim 26 , and a swollenin.28. The method of claim 26 , wherein the cellulosic material is selected from the group consisting of agricultural residue claim 26 , herbaceous material claim 26 , municipal solid waste claim 26 , pulp and paper mill residue claim 26 , waste paper claim 26 , and wood; preferably claim 26 , arundo claim 26 , bagasse claim 26 , bamboo claim 26 , corn cob claim 26 , corn fiber claim 26 , corn stover claim 26 , miscanthus claim 26 , orange peel claim 26 , rice straw claim 26 , switchgrass claim 26 , wheat straw claim 26 , eucalyptus claim 26 , fir claim 26 , pine claim 26 , poplar claim 26 , spruce claim 26 , willow claim 26 , algal cellulose claim 26 , bacterial cellulose claim 26 , cotton linter claim 26 , filter paper claim 26 , microcrystalline cellulose claim 26 , or phosphoric-acid treated cellulose.29. The method of claim 26 , wherein the cellulosic material is pretreated claim 26 , especially by chemical ...

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Номер записи: 40

28-11-2013 дата публикации

Carbon capture in fermentation

Номер: US20130316424A1

Автор: Christophe Collet, Michael Cockrem, Michael Koepke, Sean Dennis Simpson, Simon David Oakley

Принадлежит: Lanzatech New Zealand Ltd

The invention relates to methods of capturing carbon by microbial fermentation of a gaseous substrate comprising CO. The methods of the invention include converting CO to one or more products including alcohols and/or acids and optionally capturing CO2 to improve overall carbon capture. In certain aspects, the invention relates to processes for producing alcohols, particularly ethanol, from industrial waste streams, particularly steel mill off-gas.

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Номер записи: 41

02-01-2014 дата публикации

Genetic Switches for Butanol Production

Номер: US20140004526A1

Автор: Dauner Michael, Kruckeberg Arthur Leo, Larossa Robert A., Paul Brian James

Принадлежит: Butamax™ Advanced Biofuels LLC

The invention relates to suitable screening strategies for evaluating various candidate promoters for differential gene expression during the propagation and production phases of a fermentation process. The invention also relates to recombinant host cells that comprise identified promoter nucleic acid sequences and methods for producing fermentation products employing the same. 1. A method of producing butanol or 2-butanone comprising:A) contacting a recombinant host cell comprising a heterologous polynucleotide, said heterologous polynucleotide comprisingi) a promoter nucleic acid sequence; andii) a nucleic acid sequence encoding a biocatalyst polypeptide with a carbon substrate under a first set of conditions; andB) contacting the recombinant host cell with a carbon substrate under a second set of conditions;wherein the first set of conditions and second set of conditions differ and wherein the nucleic acid sequence encoding a biocatalyst polypeptide is differentially expressed under the first set of conditions than under the second set of conditions; andwherein the host cell produces butanol or 2-butanone under at least one of the first set or the second set of conditions.2. The method of wherein the first set of conditions and the second set of conditions differ in at least one of source of carbon substrate claim 1 , dissolved oxygen concentration claim 1 , temperature claim 1 , pH claim 1 , glucose concentration claim 1 , or butanol or 2-butanone concentration.3. The method of wherein the dissolved oxygen concentration is greater during the first set of conditions than during the second set of conditions.4. (canceled)5. The method of wherein the average glucose concentration is lower in the first set of conditions than during the second set of conditions.6. (canceled)7. (canceled)8. (canceled)9. The method of wherein the rate of butanol production is lower under the first set of conditions than under the second set of conditions.10. The method of wherein the ...

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Номер записи: 42

02-01-2014 дата публикации

Production of products from biomass

Номер: US20140004574A1

Автор: Aiichiro YOSHIDA, Jaewoong MOON, Marshall Medoff, Thomas Craig Masterman

Принадлежит: Xyleco Inc

The processes disclosed herein include saccharifying cellulosic and/or lignocellulosic biomass and fermenting the sugars to produce a sugar alcohol.

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Номер записи: 43

02-01-2014 дата публикации

CONDITIONING OF SO2-ETHANOL-WATER SPENT LIQUOR FOR FERMENTATION BY CLOSTRIDIA

Номер: US20140004582A1

Автор: Sklavounos Evangelos, van Heiningen Adriaan

Принадлежит: API Intellectual Property Holdings, LLC

The present invention relates to producing chemicals and biofuels from wood material, e.g. mixed forest biomass. Specifically, the invention concerns a process for conditioning spent liquor produced by SO-ethanol-water (SEW) fractionation of wood chips for fermentation to butanol, ethanol and acetone/isopropanol (so called ABE process) by Clostridia bacteria. 15-. (canceled)6. A method for conditioning of a biomass-derived liquor containing sulfur dioxide , said method comprising:(a) obtaining a first liquor derived from cooking and washing a biomass feedstock, wherein said first liquor comprises sulfur dioxide, water, hemicelluloses, and lignin;(b) evaporating and stripping said first liquor to remove a first amount of sulfur dioxide, thereby generating a second liquor;(c) increasing the pH of said second liquor to precipitate and remove a second amount of sulfur dioxide, in the form of lignosulfonate, thereby generating a third liquor comprising sulfite anions; and(d) exposing said third liquor to catalytic oxidation in the presence of a ferrous sulfate-based catalyst and an oxidant under effective reaction conditions to oxidize said sulfite anions to sulfate anions, thereby generating a conditioned liquor.7. The method of claim 6 , wherein said biomass is selected from hardwoods claim 6 , softwoods claim 6 , annual plant fibers claim 6 , or combinations thereof.8. The method of claim 6 , wherein said first liquor further comprises ethanol.9. The method of claim 6 , wherein said stripping in step (b) is steam stripping.10. The method of claim 6 , said method further comprising separating a lignin-containing precipitate during step (b).11. The method of claim 6 , wherein said pH of said second liquor is at least 7.12. The method of claim 6 , wherein during step (c) claim 6 , said pH of said second liquor is increased by addition of lime claim 6 , and wherein said lignosulfonate is calcium lignosulfonate that precipitates from said second liquor.13. The method of ...

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Номер записи: 44

09-01-2014 дата публикации

Microbial Synthesis Of Aldehydes And Corresponding Alcohols

Номер: US20140011231A1

Автор: Kwang Myung Cho, Shahrooz Rabizadeh, Wendy M. HIGASHIDE

Принадлежит: Easel Biotechnologies LLC

An improved process for alcohol production includes microbial fermentation using a genetically modified microorganism to produce substantial quantities of aldehydes that are stripped from the fermentation medium and condensed. So produced aldehydes are converted in an ex vivo process to corresponding alcohols.

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Номер записи: 45

09-01-2014 дата публикации

Method for Producing Probiotically Derived Compounds

Номер: US20140011244A1

Автор: Marshall Robert J.

Принадлежит: Premier Research Labs, LP

A method for producing naturally derived beneficial compounds including dispersing a microbiological culture media including at least one live probiotic organism, and at least one nutraceutical and/or at least one nutritive agent in distilled water to form a broth, incubating the broth at a predetermined temperature for a select period of time to induce probiotic activity; halting the probiotic activity, and separating the desired compound from the broth. 1. A process for naturally deriving a beneficial compound comprising:preparing a microbiological culture comprising at least one live probiotic organism and at least one nutritive agent or at least one nutraceutical agent;incubating the microbiological culture to initiate probiotic activity;halting the probiotic activity;harvesting a waste byproduct of the probiotic activity; andseparating the beneficial compound from the waste byproduct.2LactobacillusBifidobacteriumEnterococcusStreptococcus thermophilus. The process of wherein the at least one live probiotic organism is selected from the group consisting of species claim 1 , species claim 1 , species claim 1 , claim 1 , and combinations thereof.3. The process of wherein the microbiological culture is incubated at a temperature of from about 35° C. to about 40° C.4. The process of wherein the microbiological culture is incubated for a period of from about 24 hours to about 240 hours.5. The process of wherein the probiotic activity is halted by adding organic ethanol.6. A process for naturally deriving at least one B vitamin coenzyme comprising:{'i': Lactobacillus', 'Bifidobacterium', 'Enterococcus', 'Streptococcus thermophilus, 'preparing a microbiological culture comprising at least one live probiotic organism selected from the group consisting of species, species, species, , and combinations thereof, at least one nutritive agent, and at least one source of a B vitamin;'}incubating the microbiological culture to initiate probiotic activity;halting the probiotic ...

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Номер записи: 46

09-01-2014 дата публикации

Methods for Harvesting and Processing Biomass

Номер: US20140011246A1

Автор: Anthony Renil, Ellis Joshua T., Miller Charles, Rahman Asif, Sathish Ashik, Sims Ronald

Принадлежит: Utah State University

A system and method for harvesting and processing algae, the system and method including harvesting algae by mechanical or chemical system and processing the harvested algae to produce at least one of biodiesel, biosolvents, bioplastics, biogas, or fertilizer. 1. A system for harvesting algae , comprising:a mechanical harvesting system, anda chemical harvesting system.2. The system of claim 1 , wherein the mechanical harvesting system comprises a rotating bioreactor.3. The system of claim 1 , wherein the chemical harvesting system comprises organic coagulants.4. The system of claim 3 , wherein the organic coagulants comprise modified starch.5. A system for harvesting and processing algae claim 3 , comprising:a rotating bioreactor harvester,a chemical harvesting module,a biodiesel producing module,a biosolvent producing module,a bioplastics producing module, anda biogas and fertilizer producing module.6. A method for harvesting and processing algae claim 3 , the method comprising:harvesting algae, andprocessing algae.7. The method of claim 6 , wherein harvesting algae comprises harvesting algae with a rotating bioreactor.8. The method of claim 6 , wherein harvesting algae comprises harvesting algae with organic chemical coagulants.9. The method of claim 6 , wherein processing algae comprises wet lipid extraction.10. The method of claim 9 , wherein processing algae further comprises producing biodiesel.11. The method of claim 9 , wherein processing algae further comprises producing biosolvents.12. The method of claim 9 , wherein processing algae further comprises producing bioplastics.13. The method of claim 9 , wherein processing algae further comprises producing biogas and/or fertilizer. This application claims priority to U.S. Provisional Patent Application No. 61/657,972, filed Jun. 11, 2012, the entirety of which is hereby incorporated by reference. This application hereby incorporates by reference the following related U.S. patent application Ser. Nos.: 12/907, ...

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Номер записи: 47

23-01-2014 дата публикации

Metabolically Engineered Cells For The Production of Pinosylvin

Номер: US20140024862A1

Автор: Bjorn Sara Peterson, David Helga, Durhuus Thomas Thomasen, Forster Jochen, Katz Michael, Schmidt Hans Peter, Sendelius Malin

Принадлежит:

A genetically engineered micro-organism having an operative metabolic pathway producing cinnamoyl-CoA and producing pinosylvin therefrom by the action of a stilbene synthase is used for pinosylvin production. Said cinnamic acid may be formed from L-phenylalanine by a L-phenylalanine ammonia lyase (PAL) which is one accepting phenylalanine as a substrate and producing cinammic acid therefrom, preferably such that if the PAL also accepts tyrosine as a substrate and forms coumaric acid therefrom, the ratio Km(phenylalanine)/Km(tyrosine) for said PAL is less than 1:1 and if said micro-organism produces a cinammate-4-hydroxylase enzyme (C4H), the ratio K(PAL)/K(C4H) is at least 2:1. 163-. (canceled)64. A micro-organism having an operative metabolic pathway producing cinnamoyl-CoA and producing pinosylvin therefrom by the action of a stilbene synthase , wherein said cinnamic acid is formed from L-phenylalanine by a L-phenylalanine ammonia lyase (PAL) which is one accepting phenylalanine as a substrate and producing cinammic acid therefrom , such that if the PAL also accepts tyrosine as a substrate and forms coumaric acid therefrom , the ratio Km(phenylalanine)/Km(tyrosine) for said PAL is less than 1:1.65. A micro-organism as claimed in claim 64 , wherein if said micro-organism produces a cinammate-4-hydroxylase enzyme (C4H) claim 64 , the ratio K(PAL)/K(C4H) is at least 2:1.66. A micro-organism as claimed in claim 65 , wherein cinnamoyl-CoA is formed in a reaction catalysed by an enzyme in which ATP and CoA are substrates and ADP is a product.67. A micro-organism as claimed in claim 66 , wherein cinnamoyl-CoA is formed in a reaction catalysed by a 4-coumarate-CoA ligase or cinnamate-CoA ligase.68. A micro-organism as claimed in claim 67 , wherein said 4-coumarate-CoA ligase or cinnamate-CoA ligase is expressed in said micro-organism from nucleic acid coding for said enzyme which is not native to the micro-organism. ...

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Номер записи: 48

30-01-2014 дата публикации

APPARATUS AND METHOD FOR SEPARATING AND REFINING PRODUCT MANUFACTURED BY MICROBIAL FERMENTATION BY USING ADSORBENT

Номер: US20140030777A1

Автор: Cho Jung-Hee, Chol Jin Dal Rae, Eom Moon-Ho, Jeon Sang-Jun, Lee Julia, Lee Sang-Hyun

Принадлежит: GS CALTEX CORPORATION

The present invention relates to an apparatus and a method for fermenting, separating, and refining a product, which is produced by cultivating a microorganism. The apparatus and the method for fermenting, separating, and refining, of the present invention, can separate and refine the product that is produced by microbial fermentation in a simple, continuous manner and with high efficiency. 1. A continuous fermentation , separation , and refinement apparatus of products comprising:a supply bath supplying a medium to a column;at least two columns in which a microbe is cultured so as to produce the products and an adsorbent is filled; andat least one conversion part moving a culture medium in a first column to a second column when the products are sufficiently adsorbed in the first column to which the medium is supplied, stopping supply of the medium to the first column, and changing a flow of the medium so as to supply the medium to the second column.2. The continuous fermentation claim 1 , separation claim 1 , and refinement apparatus of claim 1 , wherein the case in which the products are sufficiently adsorbed is a case in which an adsorption rate of the product with respect to the adsorbent is decreased claim 1 , a case in which growth of the microbe in the column is inhibited by an increase in the concentration of the product claim 1 , or a case in which productivity of the product of the microbe is decreased.3. The continuous fermentation claim 1 , separation claim 1 , and refinement apparatus of claim 1 , wherein the product is alcohol claim 1 , ketone claim 1 , ester claim 1 , or carboxylic acid.4. The continuous fermentation claim 1 , separation claim 1 , and refinement apparatus of claim 1 , wherein the medium contains a raw material claim 1 , and the raw material is fatty acid or sugars.5. The continuous fermentation claim 1 , separation claim 1 , and refinement apparatus of claim 1 , wherein the culture medium circulates between the columns.6. The ...

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Номер записи: 49

06-02-2014 дата публикации

APPARATUS AND METHOD FOR SEPARATING AND REFINING PRODUCT MANUFACTURED BY MICROBIAL FERMENTATION BY USING ADSORBENT

Номер: US20140038250A1

Автор: Cho Jung-Hee, Choi Jin Dal Rae, Eom Moon-Ho, Jeon Sang-Jun, Lee Julia, Lee Sang-Hyun

Принадлежит: GS CALTEX CORPORATION

The present invention relates to an apparatus and a method for fermenting, separating, and refining a product, which is produced by cultivating a microorganism. The apparatus and the method for fermenting, separating, and refining, of the present invention, can separate and refine the product that is produced by microbial fermentation in a simple, continuous manner and with high efficiency. 1. A continuous fermentation , separation , and refinement apparatus of products comprising:a culture bath in which a microbe is cultured together with a raw material to produce the products;at least two columns filled with an adsorbent; anda conversion part controlling so that a culture medium containing the products is supplied from the culture bath to a specific column,wherein the conversion part stops supplying the culture medium to a first column in the case in which the products are sufficiently adsorbed in the first column supplied with the culture medium and changes a flow of the culture medium so as to supply the culture medium to a second column.2. The continuous fermentation claim 1 , separation claim 1 , and refinement apparatus of claim 1 , wherein the case in which the products are sufficiently adsorbed is a case in which an adsorption rate of the product with respect to the adsorbent is decreased or a case in which a concentration of the product in a discharge solution discharged from the column is 80% or more of a concentration of the product in the culture medium supplied to the column.3. The continuous fermentation claim 1 , separation claim 1 , and refinement apparatus of claim 1 , wherein at least some of the discharge solution discharged from the column is supplied to the culture bath.4. The continuous fermentation claim 3 , separation claim 3 , and refinement apparatus of claim 3 , wherein the case in which the products are sufficiently adsorbed is a case in which an adsorption rate of the product with respect to the adsorbent is decreased claim 3 , a case ...

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Номер записи: 50

13-02-2014 дата публикации

Novel Algae Extraction Methods

Номер: US20140045217A1

Автор: Lindner Tanja, Milos Klaudija, Scheidig Andreas, Stamme Claudia, Svetlichny Vitaly

Принадлежит: DIREVO INDUSTRIAL BIOTECHNOLOGY GmbH

The present disclosure provides novel methods and compositions for improved production processes of algae cell components. In particular the present disclosure relates to methods for improving the extractability of algae cellular compositions. 1. A method for producing an algae cell component comprising the steps of:a) culturing and growing algae in a liquid medium to a desired algae biomass,b) harvesting the algae,c) enzymatic treatment of the algae with an enzyme composition comprising a mannanase and a lipase, andd) isolating the cell component from the algae biomass.2. The method to claim 1 , whereby the cellular component is selected from the group consisting of pigments claim 1 , carotenoids claim 1 , starch claim 1 , lipids claim 1 , poly unsaturated fatty acids (PUFA) claim 1 , proteins claim 1 , vitamins and mineral nutrients.3. The method according to claim 1 , whereby the cellular composition is a carotenoid.4. The method according to claim 3 , whereby the carotenoid is astaxanthin.5. The method according to claim 4 , whereby the astaxanthin is in form of a (3S claim 4 , 3′S) isomer.6. The method according to claim 1 , whereby the enzyme composition further comprises at least an enzyme selected from the group consisting of proteases claim 1 , cellulases claim 1 , pectinases and glucanases claim 1 , or mixture thereof.7. The method according to claim 1 , whereby the enzyme composition further comprises an endo-1 claim 1 ,3(4)-beta glucanase.8. The method according to claim 1 , whereby the enzyme composition further comprises a laminarinase.9. The method according to claim 1 , whereby the algae are green algae.10haematococcus.. The method according to claim 9 , whereby the green algae are algae of the class chlorophyceae claim 9 , preferably of the order voivocales claim 9 , preferably of the family haematococcaceae claim 9 , more preferably of the genus11Haematococcus pluvialis.. The method according to claim 10 , whereby the algae are of the species12. A ...

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Номер записи: 51

13-02-2014 дата публикации

Method for Biotechnological Production of Dihydrochalcones

Номер: US20140045233A1

Автор: Beutel Sascha, BORNSCHEUER UWE, Gall Mechthild, Gross Egon, Hilmer Jens Michael, Jonczyk Patrick, Krammer Gerhard, Ley Jakob Peter, Peters Christin, Scheper Thomas, Thomsen Maren

Принадлежит:

A method for production of a dihydrochalcone, especially of phloretin, using a transgenic microorganism, containing a nucleic acid section (a), comprising or consisting of a gene coding for a bacterial chalcone isomerase, and/or a nucleic acid section (a′), comprising or consisting of a gene coding for a plant chalcone isomerase, and a nucleic acid section (b), comprising or consisting of a gene coding for a bacterial enoate reductase, corresponding transgenic microorganisms, containing a nucleic acid section (a), comprising or consisting of a gene coding for a bacterial chalcone isomerase, and/or a nucleic acid section (a′), comprising or consisting of a gene coding for a plant chalcone isomerase, and/or a nucleic acid section (b), comprising or consisting of a gene coding for a bacterial enoate reductase, and host cells, containing one or more identical or different such vectors. 1. Method for production of a dihydrochalcone using a transgenic microorganism comprising: a nucleic acid section (a), comprising or consisting of a gene coding for a bacterial chalcone isomerise;', 'and/or a nucleic acid section (a′), comprising or consisting of a gene coding for a plant chalcone isomerise;', 'and', 'a nucleic acid section (b), comprising or consisting of a gene coding for a bacterial enoate reductase;, '(i) providing a transgenic microorganism, containing'}(ii) adding one or more flavanones and optionally of one or more precursors or one or more derivatives thereof to the transgenic microorganism and cultivation of the transgenic microorganism under conditions which allow the conversion of the flavanone(s) and/or the precursor(s) or of the derivative(s) thereof to a dihydrochalcone; and(iii) optionally isolating and purifying the dihydrochalcone.2. The method of claim 1 , wherein:the dihydrochalcone is phloretin; andthe flavanones are naringin.3. Method of claim 1 , wherein the transgenic microorganism is not a microorganism of the phylum Firmicutes.4E. coli, E. coliE. ...

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Номер записи: 52

27-02-2014 дата публикации

CONTROLLED ACTIVATION OF THE REUTERIN-PRODUCTION MACHINERY OF LACTOBACILLUS

Номер: US20140057992A1

Автор: Roos Stefan

Принадлежит:

Methods for the controlled activation of the reuterin-production machinery of by adding glycerol and other substances during the manufacture of cell-cultures and keeping the produced reuterm in the bacterial cell during preservation and storage, in particular this invention relates to the manufacture of large amounts of that are loaded with reuterm, and the use of such loaded bacteria for applications such as prevention and treatment of diseases, for food applications and the like. 115-. (canceled)16Lactobacillus reuteri. A product produced by a method for the controlled activation of the reuterin-production machinery of cell cultures comprising:{'i': 'Lactobacillus reuteri', 'manufacturing cell cultures{'i': 'Lactobacillus reuteri', 'adding glycerol to the cell cultures during manufacture, thereby producing reuterin: and'}{'i': Lactobacillus reuteri', 'Lactobacillus reuteri., 'keeping the produced reuterin inside the cell cultures during the preservation and storage of the'}1713Lactobacillus reuteri. A product produced by a the method of claim for the controlled activation of the reuterin-production machinery of cell cultures , comprising:{'i': 'Lactobacillus reuteri', 'claim-text': fermenting the cell cultures:', 'washing the cell cultures: and', 'freeze-drying the cell cultures: and, 'wherein the manufacture of the cell cultures comprises, 'manufacturing cell cultures{'i': Lactobacillus reuteri', 'Lactobacillus reuteri', 'Lactobacillus reuteri., 'adding about 1 to about 500 mM of glycerol to the cell cultures prior to freeze-drying the cell cultures thereby producing reuterin, thereby keeping the produced reuterin inside the cell cultures during preservation and storage of the'}18. A method for preventing or treating disorders induced by pathogens of the cutaneous system comprising the administration of the product of .19. The method of wherein the administration is nasal administration.20. The method of wherein the method is for preventing or treating MRSA. This ...

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Номер записи: 53

13-03-2014 дата публикации

METHOD AND APPARATUS FOR RECYCLING AND REUSING BEVERAGES AND OTHER LIQUIDS AND CONTAINER MATERIALS

Номер: US20140073025A1

Автор: DAVIS TOMMY MACK

Принадлежит: TMD TECHNOLOGIES GROUP, LLC

A system for destruction and recycling of containers (such as bottles, cans and the like), as well as associated liquids, food products or other residue, greatly reduces waste discharge and/or disposal requirements. Packaging and other solids are shredded, ground or otherwise processed, while associated liquids are collected. Evaporation removes water for reuse, thus concentrating sugars and other food materials for utilization as agricultural amendments or stock for bio-product production. Bio-fuels/solvents are generated, typically using an Immobilized Microbe Bioreactor system modified for fuels/solvents production. 1. A method for recycling solid containers , beverages or other liquids comprising:a) receiving a waste materials comprising at least one solid container and at least one liquid;b) separating said at least one solid container from said at least one liquid;c) concentrating sugars from said at least one liquids to create feed stock; andd) generating bio-fuel from said feed stock.2. The method of claim 1 , wherein said step of separating said at least one solid container from said at least one liquid further comprises cutting said at least one solid container.3. The method of claim 2 , wherein said step of separating said at least one solid container from said at least one liquid further comprises collecting said at least one liquid in at least one catchment basin.4. The method of claim 1 , wherein said step of concentrating sugars from said at least one liquid to create feed stock comprises evaporating water from said at least one liquid.5. The method of claim 1 , wherein said step of generating bio-fuel from said feed stock comprises fermentation.6. The method of claim 5 , wherein said fermentation is performed using at least one immobilized microbe bioreactor.7. The method of claim 5 , wherein solids from the fermentation process are directed to an aerobic or anaerobic digester.8. The method of claim 7 , wherein gases produced in anaerobic digestion ...

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Номер записи: 54

13-03-2014 дата публикации

VALENCENE SYNTHASE

Номер: US20140075600A1

Автор: ACHKAR Jihane, BEEKWILDER Martinus Julius, Bosch Hendrik Jan, Bouwmeester Hendrik Jan, SONKE Theodorus

Принадлежит: Isobionics B.V.

The present invention relates to a valencene synthase, to a nucleic acid encoding such valencene synthase, to a host cell comprising said encoding nucleic acid sequence and to a method for preparing valencene, comprising converting farnesyl diphosphate to valencene in the presence of a valencene synthase according to the invention. 1. A valencene synthase comprising an amino acid sequence as shown in SEQ ID NO: 2 , SEQ ID NO: 4 , or a functional homologue of any of these sequences , said homologue being a valencene synthase comprising an amino acid sequence which has a sequence identity of at least 40% with SEQ ID NO: 2 or SEQ ID NO: 4.2. The valencene synthase according to claim 1 , having at least 60% claim 1 , at least 70% claim 1 , at least 80% claim 1 , at least 90% claim 1 , at least 95% claim 1 , or at least 98% sequence identity with SEQ ID NO: 2 or SEQ ID NO: 4.3. A nucleic acid claim 1 , comprising a nucleic acid sequence encoding a valencene synthase according to claim 1 , a complementary sequence thereof claim 1 , or comprising a nucleic acid sequence hybridising with a nucleic acid sequence encoding a valencene synthase according to under stringent conditions.4. A nucleic acid according to claim 3 , wherein the nucleic acid comprises a nucleic acid sequence as shown in SEQ ID NO: 1 claim 3 , SEQ ID NO: 3 claim 3 , SEQ ID NO: 18 claim 3 , SEQ ID NO: 19 or another nucleic acid sequence encoding a valencene synthase comprising a nucleic acid sequence having a sequence identity of at least 40% claim 3 , at least 60% claim 3 , at least 70% claim 3 , at least 80% claim 3 , at least 90% or at least 95% with any of the sequences shown in SEQ ID NO: 1 claim 3 , SEQ ID NO: 3 claim 3 , SEQ ID NO: 18 claim 3 , or SEQ ID NO: 19.5. An expression vector comprising a nucleic acid according to .6. A host cell claim 5 , which may be an organism per se or part of a multi-cellular organism claim 5 , said host cell comprising an expression vector according to claim 5 , ...

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Номер записи: 55

27-03-2014 дата публикации

Microorganisms And Methods For Producing Substituted Phenols

Номер: US20140087428A1

Автор: Fleige Christian, Hansen Gunda, Hilmer Jens-Michael, Kroll Jens, Lambrecht Stefan, PESARO Manuel, Steinbüchel Alexander

Принадлежит:

The present invention is concerned with methods for producing vanillin, feroyl-CoA, ferulic acid, coniferyl aldehyde and/or coniferyl alcohol. Also, the invention relates to microorganisms useful in such production method, and to the construction of such microorganisms. 1. Method for producing a product selected from the group consisting of vanillin , feruloyl-CoA , ferulic acid , coniferyl aldehyde and coniferyl alcohol , comprising adding an educt selected from the group consisting of eugenol , coniferyl alcohol , coniferyl aldehyde and ferulic acid to a microorganism of order Actinomycetales , wherein the microorganism does not comprise a gene coding for a vanillin dehydrogenase.2. The method according to claim 1 , wherein the vanillin dehydrogenase has an amino acid sequence according to any of SEQ ID 1 claim 1 , 2 or 3.3. The method according to claim 1 , wherein the microorganism comprises a vanillin dehydrogenase gene inactivated by an insert or a deletion.4. The method according to claim 1 , wherein the microorganism comprises a gene selected from the group consisting of:{'i': Pseudomonas', 'Penicillium simplicissimium', 'Rhodococcus jostii, 'an eugenol hydroxylase gene of sp. DSMZ 7063, a vanillyl alcohol oxidase gene of , and an eugenol oxidase gene of RHA1,'}{'i': Pseudomonas', 'Rhodococcus opacus', 'Acinetobacter', 'Rhodococcus erythropolis', 'Lactobacillus plantarum', 'Gordonia polyisoprenivorans, 'sub': —', '—, 'a coniferyl alcohol dehydrogenase gene of sp. DSMZ 7063, an aromatic alcohol dehydrogenase gene (adhA; accession number AB213394) of TKN14, an aryl alcohol dehydrogenase gene of sp. ADP1, an aryl alcohol dehydrogenase gene of PR4, WCFS 1 (lp3054; accession number CAB69495) and Kd2 (adhA, accession number ZP09272609.1),'}{'i': Pseudomonas', 'Acinetobacter', 'Arabidopsis thaliana', 'Pseudomonas putida', 'Gluconobacter oxydans, 'sub': —', '—, 'a coniferyl aldehyde dehydrogenase of sp. DSMZ 7063, a benzaldehyd dehydrogenase of sp. ADP1, an aldehyd- ...

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Номер записи: 56

03-04-2014 дата публикации

Variant Sucrose Transporter Polypeptides That Enable Faster Sucrose Utilization In Bacteria

Номер: US20140093927A1

Автор: Chen Qi, CHENG Qiong, LAI Jian Ping, RUEBLING-JASS KRISTIN

Принадлежит: E. I. DU PONT DE NEMOURS AND COMPANY

Variant sucrose transporter polypeptides that enable faster sucrose utilization in bacteria are described. Additionally, variant or recombinant bacteria comprising these variant sucrose transporter polypeptides, and methods of utilizing the bacteria to produce products such as glycerol and glycerol-derived products are provided. 1. (canceled)3. The bacterium of claim 2 , wherein the polypeptide having sucrose hydrolase activity is classified as EC 3.2.1.26 or EC 2.4.1.7.4. The bacterium of further comprising in its genome or on at least one recombinant construct claim 2 , a nucleotide sequence encoding a polypeptide having fructokinase activity.5. The bacterium of claim 4 , wherein the polypeptide having fructokinase activity is classified as EC 2.7.1.4 claim 4 , EC 2.7.1.3 claim 4 , or EC 2.7.1.1.6Escherichia, Klebsiella, CitrobacterAerobacter.. The bacterium of wherein said bacterium is selected from the group consisting of the genera: claim 2 , and7Escherichia coli.. The bacterium of wherein said bacterium is8. The bacterium of wherein the bacterium is a recombinant bacterium that produces 1 claim 2 ,3-propanediol claim 2 , glycerol claim 2 , and/or 3-hydroxypropionic acid.9. A process for making glycerol claim 2 , 1 claim 2 ,3-propanediol and/or 3-hydroxypropionic acid from sucrose comprising:{'claim-ref': {'@idref': 'CLM-00008', 'claim 8'}, 'a) culturing the recombinant bacterium of in the presence of sucrose; and'}b) recovering the glycerol, 1,3-propanediol and/or 3-hydroxypropionic acid produced. The invention relates to the fields of microbiology and molecular biology. More specifically, variant sucrose transporter polypeptides that enable faster sucrose utilization in bacteria, variant or recombinant bacteria comprising these variant sucrose transporter polypeptides, and methods of utilizing such bacteria to produce products such as glycerol and glycerol-derived products are provided.Many commercially useful microorganisms use glucose as their main ...

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Номер записи: 57

07-01-2016 дата публикации

Polypeptides Having Peroxygenase Activity and Polynucleotides Encoding Same

Номер: US20160002683A1

Автор: Lars Henrik Oestergaard, Lisbeth Kalum, Sara Landvik

Принадлежит: Novozymes AS

The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

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Номер записи: 58

05-01-2017 дата публикации

PROCESSES FOR FERMENTATION OF LIGNOCELLULOSIC GLUCOSE TO ALIPHATIC ALCOHOLS OR ACIDS

Номер: US20170002387A1

Автор: PYLKKANEN Vesa, RETSINA Theodora

Принадлежит:

A process for producing an organic aliphatic product (such as butanol) from lignocellulosic biomass is provided, comprising: (a) fractionating lignocellulosic biomass in the presence of a solvent for lignin, a hydrolysis catalyst, and water, to produce a liquor containing hemicellulose, cellulose-rich solids, and lignin; (b) washing the cellulose-rich solids and separating the cellulose-rich solids from the liquor; (c) enzymatically hydrolyzing the cellulose-rich solids to generate a hydrolysate comprising glucose; (d) detoxifying the hydrolysate by neutralizing the hydrolysate, removing insoluble solids, and removing or oxidizing residual hydrolysis catalyst, thereby generating a purified hydrolysate; (e) fermenting the purified hydrolysate using a suitable microorganism to produce a dilute organic aliphatic product, wherein the microorganism is recycled with a membrane; (f) extracting the dilute organic aliphatic product into a water-immiscible extractant, to generate an intermediate material; and (g) distilling the intermediate material to generate a concentrated organic aliphatic product. 1. A process for producing an organic aliphatic product from lignocellulosic biomass , said process comprising:(a) fractionating a feedstock comprising lignocellulosic biomass in the presence of a solvent for lignin, a hydrolysis catalyst, and water, to produce a liquor containing hemicellulose, cellulose-rich solids, and lignin;(b) washing said cellulose-rich solids and separating said cellulose-rich solids from said liquor;(c) enzymatically hydrolyzing said cellulose-rich solids to generate a hydrolysate comprising glucose;(d) detoxifying said hydrolysate by neutralizing said hydrolysate, removing insoluble solids, and removing or oxidizing residual hydrolysis catalyst, thereby generating a purified hydrolysate;(e) fermenting said purified hydrolysate using a suitable microorganism in a fermentor to produce a dilute organic aliphatic product, wherein said microorganism is ...

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Номер записи: 59

02-01-2020 дата публикации

HIERARCHICAL MAGNETIC NANOPARTICLE-ENZYME MESOPOROUS ASSEMBLIES EMBEDDED IN MACROPOROUS SCAFFOLDS

Номер: US20200002698A1

Автор: ANESHANSLEY Daniel, CORGIE Stephane C., Duan Xiaonan, Giannelis Emmanuel, WALKER Larry P.

Принадлежит: CORNELL UNIVERSITY

A hierarchical catalyst composition comprising a continuous or particulate macroporous scaffold in which is incorporated mesoporous aggregates of magnetic nanoparticles, wherein an enzyme is embedded in mesopores of the mesoporous aggregates of magnetic nanoparticles. Methods for synthesizing the hierarchical catalyst composition are also described. Also described are processes that use the recoverable hierarchical catalyst composition for depolymerizing lignin remediation of water contaminated with aromatic substances, polymerizing monomers by a free-radical mechanism, epoxidation of alkenes, halogenation of phenols, inhibiting growth and function of microorganisms in a solution, and carbon dioxide conversion to methanol. Further described are methods for increasing the space time yield and/or total turnover number of a liquid-phase chemical reaction that includes magnetic particles to facilitate the chemical reaction, the method comprising subjecting the chemical reaction to a plurality of magnetic fields of selected magnetic strength, relative position in the chemical reaction, and relative motion. 122.-. (canceled)23. A method for epoxidation reactions of alkenes , the method comprising reacting alkenes in the presence of oxygen with a hierarchical catalyst composition comprising a continuous macroporous scaffold in which is incorporated self-assembled mesoporous aggregates of magnetic nanoparticles containing an oxygen-transfer enzyme embedded in mesopores of said mesoporous aggregates of magnetic nanoparticles , to produce an alkene oxide.24. The method of claim 23 , wherein said oxygen-transfer enzyme is a chloroperoxidase or a lipase.2539.-. (canceled)40. The method of claim 23 , further comprising magnetic particles claim 23 , not belonging to said mesoporous aggregates of magnetic nanoparticles claim 23 , embedded in said continuous macroporous scaffold.41. The method of claim 23 , wherein said continuous macroporous scaffold has a polymeric composition.42 ...

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Номер записи: 60

04-01-2018 дата публикации

HETEROTROPHIC PRODUCTION METHODS FOR MICROBIAL BIOMASS AND BIOPRODUCTS

Номер: US20180002711A1

Автор: KUEHNLE ADELHEID R., SCHURR ROBERT J.

Принадлежит:

The invention pertains to a method for synthesizing a product of interest by culturing a microalgal cell producing the product of interest in the dark in a culture medium comprising an organic acid as a fixed carbon source, wherein the microalgal cell is a facultative heterotroph. The product of interest can be a microalgal biomass, a pigment, terpene, recombinant molecule, biogas, or a precursor thereof. In an embodiment, the culture medium comprises urea as a primary source of nitrogen. In one embodiment, the microalgal cell belongs to the order Chlamydomonadales. A method of identifying and isolating a microalgal cell having a preferred characteristic that is suitable for synthesis of a product of interest is also provided, the method comprising identifying and isolating a non-mutagenized or recombinant microalgal cell from a microalgal culture using a fluorescence activated cell sorting technique and/or a phototaxic response. 1. A method for synthesizing a product of interest , the method comprising:providing a culture medium comprising an organic acid as a fixed carbon source;providing a microalgal cell that produces the product of interest, wherein the microalgal cell is a facultative heterotroph;culturing the microalgal cell in the culture medium in the dark to produce a microalgal culture from the microalgal cell;isolating the microalgal cells from the microalgal culture before the cells in the microalgal culture undergo cell differentiation; andpurifying the product of interest from the microalgal cells.2. The method of claim 1 , wherein the microalgal cell is a facultative heterotroph that is rendered an obligate heterotroph.3. The method of claim 1 , wherein the product of interest is a microalgal biomass comprising the microalgal cells.4. The method of claim 1 , wherein the product of interest is a pigment claim 1 , terpene claim 1 , recombinant molecule claim 1 , biogas claim 1 , or a precursor thereof.5. The method of claim 4 , wherein the pigment is a ...

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Номер записи: 61

04-01-2018 дата публикации

Methods, reagents and cells for biosynthesizing compounds

Номер: US20180002729A1

Автор: Adriana Leonora Botes, Alex van Eck CONRADIE, Ramdane Haddouche

Принадлежит: Invista North America LLC

This document describes biochemical pathways for producing 7-hydroxyheptanoate methyl ester and heptanoic acid heptyl ester using one or more of a fatty acid O-methyltransferase, an alcohol O-acetyltransferase, and a monooxygenase, as well as recombinant hosts expressing one or more of such exogenous enzymes. 7-hydroxyheptanoate methyl esters and heptanoic acid heptyl esters can be enzymatically converted to pimelic acid, 7-aminoheptanoate, 7-hydroxyheptanoate, heptamethylenediamine, or 1,7-heptanediol.

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Номер записи: 62

02-01-2020 дата публикации

Carbon capture in fermentation

Номер: US20200002734A1

Автор: Christophe Collet, Michael Cockrem, Michael Koepke, Sean Dennis Simpson, Simon David Oakley

Принадлежит: Lanzatech New Zealand Ltd

The invention relates to a steel mill adapted to provide gas stream(s) comprising CO to a microbial fermentation, the steel mill comprising a steel mill structure containing apparatus for a steel manufacturing process wherein said apparatus produces waste gases during various stages of the steel making process, said waste gases being directed into the atmosphere by a waste stack, wherein the waste stack is connected to a fermentation system by a transfer means connected to the waste stack to divert at least a portion of the waste gases to the microbial fermentation system.

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Номер записи: 63

07-01-2021 дата публикации

MUTANT TYPE 2-DEOXY-SCYLLO-INOSOSE SYNTHASE

Номер: US20210002687A1

Автор: MIYAZAWA Daisuke, TAKAKU Hiroaki, Wada Mitsufumi, YAMAZAKI Harutake

Принадлежит:

A polypeptide includes, in the amino acid sequence of SEQ ID NO: 1 or a similar sequence, at least one specific amino acid substitution on at least one of the 14th, 37th, 209th, 293rd, and 319th amino acid residues from the N-terminal of the amino acid sequence of SEQ ID NO: 1. A polynucleotide, an expression cassette, a vector, and a transformant include a base sequence encoding the amino acid sequence of the polypeptide. A method of producing the polypeptide and a method of producing 2-deoxy-scyllo-inosose are also provided. 1. A polypeptide comprising at least one amino acid mutation selected from the group consisting of the following (a) to (e) in an amino acid sequence of the following (A1) or (A2):(A1) an amino acid sequence of SEQ ID NO: 1;(A2) an amino acid sequence of a polypeptide having an enzymatic activity that produces 2-deoxy-scyllo-inosose from glucose 6-phosphate, the amino acid sequence (A2) having a sequence identity of 80% or higher with the amino acid sequence of SEQ ID NO: 1,(a) an amino acid mutation in which an amino acid residue corresponding, in terms of alignment, to an asparagine residue that is a 14th amino acid residue from the N-terminal in the amino acid sequence of SEQ ID NO: 1 is substituted with threonine;(b) an amino acid mutation in which an amino acid residue corresponding, in terms of alignment, to a tyrosine residue that is a 37th amino acid residue from the N-terminal in the amino acid sequence of SEQ ID NO: 1 is substituted with phenylalanine;(c) an amino acid mutation in which an amino acid residue corresponding, in terms of alignment, to an alanine residue that is a 290th amino acid residue from the N-terminal in the amino acid sequence of SEQ ID NO: 1 is substituted with threonine;(d) an amino acid mutation in which an amino acid residue corresponding, in terms of alignment, to a tryptophan residue that is a 293rd amino acid residue from the N-terminal in the amino acid sequence of SEQ ID NO: 1 is substituted with ...

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Номер записи: 64

03-01-2019 дата публикации

POLYPEPTIDES HAVING DEMETHYLATING ACTIVITY

Номер: US20190002846A1

Автор: Benjelloun-Mlayah Bouchra, Crestini Claudia, Frommhagen Matthias, Gruppen Harm, Iancu Svetlana Laura, Kabel Mirjam Anna, Koetsier Martijn Johannes, Lange Heiko, Visser Jacob

Принадлежит:

The present invention relates to fungal enzymes, more in particular of polyphenoloxidases (PPOs), and is based on a newly discovered enzymatic activity of a class of PPOs, i.e. de-methylation of R-substituted mono- or di-methoxyphenolsuch as present in lignin, lignin derived compounds and/or in lignocellulosic biomass. This newly discovered enzymatic activity renders these enzymes highly suitable for a plethora of applications in industry. Provided herein are methods of demethylation, processes to increase the reactivity of lignin or lignin-comprising biomass, processes of conversion lignin or lignin comprising biomass to value added products, processes for degrading and/or modifying (hemi-)cellulose in a hemicellulose-comprising substrate, and expression vectors, host cells and liquids, pastes or solid formulations and compositions for use in the demethylation method of the invention. 2. A polyphenoloxisase according to that comprises or consists of an amino acid sequence that is at least 70% identical to the amino acid sequence of at least one of SEQ ID NO: 37 claim 1 , 41 and 45.3. A polyphenoloxisase according to or that is an enzyme that releases methanol from a R-substituted di-methoxyphenol represented by formula [1].4Myceliophthora.. A polyphenoloxisase according to any one of the preceding claims that is obtainable from a fungus claim 1 , preferably a5Myceliophthora thermophila C. A polyphenoloxisase according to any one of the preceding claims that is obtainable from 1.6. A polyphenoloxisase according to any one of the preceding claims claim 1 , wherein R is an organic moiety.7. A method of demethylation of an R-substituted mono- or di-methoxyphenol represented by formula [1] claim 1 , [2] claim 1 , or [3] claim 1 , comprising the step of contacting a substrate comprising said R-substituted mono- or di-methoxyphenol with a polyphenoloxidase of any one of the - claim 1 , wherein R can be any single atom or chemical moiety.8. A method according to claim 7 , ...

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Номер записи: 65

01-01-2015 дата публикации

Valencene Synthase Polypeptides, Encoding Nucleic Acid Molecules And Uses Thereof

Номер: US20150007368A1

Автор: Dayal Saran, Grace Eunyoung Park

Принадлежит: Allylix Inc

Provided are valencene synthase polypeptides, nucleic acid molecules encoding the valencene synthases, host cells containing the nucleic acids and methods for producing products whose production is catalyzed by the polypeptides. Also provided are methods for producing valencene and nootkatone.

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Номер записи: 66

08-01-2015 дата публикации

Methods for producing end-products from carbon substrates

Номер: US20150010970A1

Автор: Gopal K. Chotani, Jayarama K. Shetty, Jeff P. Pucci, Karl J. Sanford, Manoj Kumar

Принадлежит: DANISCO US INC

The present invention provides means for the production of desired end-products of in vitro and/or in vivo bioconversion of biomass-based feed stock substrates, including but not limited to such materials as starch and cellulose. In particularly preferred embodiments, the methods of the present invention do not require gelatinization and/or liquefaction of the substrate.

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Номер записи: 67

11-01-2018 дата публикации

MICROBIAL PRODUCTION OF N-BUTYRALDEHYDE

Номер: US20180010155A1

Автор: Cho Kwang Myung, HIGASHIDE Wendy, Lee Chrissie, RABIZADEH Shahrooz

Принадлежит: Easel Biotechnologies, LLC

Microorganisms and methods of producing n-butyraldehyde with enhanced yields are presented in which a microorganism is engineered to enhance the conversion of a carbon source into n-butyraldehyde. The n-butyraldehyde is recovered by way of a gas stripping process that occurs during the conversion process, providing significantly greater product yield than post-fermentation recovery of n-butyraldehyde alone. 2. The microorganism of claim 1 , wherein the acetyl-CoA acetyltransferase has an E.C. number of 2.3.1.9.3. The microorganism of claim 1 , wherein the 3-hydroxyacyl-CoA dehydrogenase has an E.C. number of 1.1.1.157.4. The microorganism of claim 1 , wherein the crotonyl-CoA hydratase has an E.C. number of 4.2.1.55.5. The microorganism of claim 1 , wherein the butyryl-CoA dehydrogenase has an E.C. number of 1.3.99.2.6. The microorganism of claim 1 , wherein the trans-enoyl-CoA reductase has an E.C. number of 1.3.1.38.7. The microorganism of claim 1 , wherein the butanal dehydrogenase has an E.C. number of 1.2.1.57.8. The microorganism of claim 1 , wherein the at least one native gene is selected from the group consisting of ldhA claim 1 , adhE claim 1 , frdBC claim 1 , pta claim 1 , and yqhD.9. The microorganism of claim 1 , wherein the at least one native gene are ldhA claim 1 , adhE claim 1 , and frdBC.10. The microorganism of claim 1 , wherein the at least one native gene are ldhA claim 1 , adhE claim 1 , frdBC claim 1 , pta claim 1 , and yqhD.11. The microorganism of claim 1 , wherein at least one native gene is deleted to reduce alcohol production by at least 70%.12. The microorganism of claim 1 , wherein at least one native gene is deleted to reduce alcohol production by at least 90%.13. The microorganism of claim 1 , wherein the genetically modified microorganism produces a cumulative yield of 2.0 g/L n-butyraldehyde at or before 40 hours of culture time.14. The microorganism of claim 1 , wherein the genetically modified microorganism produces 50% of a ...

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Номер записи: 68

14-01-2021 дата публикации

AGARASE-3,6-ANHYDRO-L-GALACTOSIDASE-ARABINOSE ISOMERASE ENZYME COMPLEX AND METHOD FOR PRODUCTION OF TAGATOSE FROM AGAR USING THE SAME

Номер: US20210009982A1

Автор: Han Sung Ok, HYEON Jeong Eun, JEONG Da-Woon

Принадлежит: KOREA UNIVERSITY RESEARCH AND BUSINESS FOUNDATION

The present disclosure relates to an enzyme complex of arabinose isomerase, agarase and 3,6-anhydro galactosidase and a method for producing tagatose by degrading agar using the same. By using the enzyme complex according to the present disclosure, agar obtained from marine biomass can be degraded effectively and useful physiologically active substances such as tagatose can be obtained effectively therefrom. 1. An agarase complex wherein:a fusion protein 1 in which a monosaccharide convertase and a dockerin module are bound;a fusion protein 2 in which agarase and a dockerin module are bound; anda fusion protein 3 in which 3,6-anhydro-L-galactosidase and a dockerin module are bound;are linked via dockerin-cohesin binding by a mini scaffold protein comprising a cohesin module.2Lactobacillus.. The enzyme complex according to claim 1 , wherein the monosaccharide convertase is arabinose isomerase derived from3. The enzyme complex according to claim 2 , wherein the arabinose isomerase comprises amino acid sequence of SEQ ID NO 1.4. The enzyme complex according to claim 1 , wherein the dockerin is derived from cellulase.5. The enzyme complex according to claim 4 , wherein the dockerin is encoded nucleotide sequence of SEQ ID NO 35.6. The enzyme complex according to claim 4 , wherein the cellulase is selected from a group consisting of endo-β-1 claim 4 ,4-glucanase B claim 4 , endo-β-1 claim 4 ,4-xylanase B and exo-glucanase S.7Pseudomonas, SaccharophagusAleromonas.. The enzyme complex according to claim 1 , wherein the agarase is derived from one selected from a group consisting of and8. The enzyme complex according to claim 7 , wherein the agarase is β-agarase.9. The enzyme complex according to claim 8 , wherein the β-agarase is encoded nucleotide sequence of SEQ ID NO 26.10Zobellia.. The enzyme complex according to claim 1 , wherein the 3 claim 1 ,6-anhydro-L-galactosidase is derived from11. The enzyme complex according to claim 10 , wherein the 3 claim 10 ,6-anhydro-L- ...

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Номер записи: 69

10-01-2019 дата публикации

METHODS OF MAKING CAPSINOIDS BY BIOSYNTHETIC PROCESSES

Номер: US20190010522A1

Автор: Chen Hui, Yu Xiaodan

Принадлежит: Conagen Inc.

Provided herein are methods of making capsinoids including providing a capsiate synthase in a mixture or cellular system, feeding 8-methyl-6-nonenoyl-CoA, 6E-8-methylnonenoic acid or 8-methylnonanoic acid into the mixture or cellular system, feeding vanillyl alcohol into the mixture or cellular system, and collecting capsinoids from the mixture or cellular system. 1. A method of producing a capsinoid , the method comprising:(a) expressing a capsiate synthase (CS) in a cellular system;(b) adding 8-methyl-6-nonenoyl-CoA and vanillyl alcohol to the cellular system; and(c) incubating the cellular system for a sufficient time to produce the capsinoid.2. A method of producing dihydrocapsiate , the method comprising:(a) expressing a capsiate synthase (CS) and an acyltransferase (ACS) in a cellular system;(b) adding 8-methylnonanoic acid and vanillyl alcohol to the cellular system; and(c) incubating the cellular system for a sufficient time to produce the dihydrocapsiate.3. A method of producing capsiate , the method comprising:(a) expressing a capsiate synthase (CS) and an acyltransferase (ACS) in a cellular system;(b) adding 6E-8-methylnonanoic acid and vanillyl alcohol to the cellular system; and(c) incubating the cellular system for a sufficient time to produce the capsiate.4. A method of producing a capsinoid , the method comprising:(a) expressing a capsiate synthase (CS) and an acyltransferase (ACS) in a cellular system;(b) adding a medium chain fatty acid and vanillyl alcohol to the cellular system; and(c) incubating the cellular system for a sufficient time to produce the capsinoid.5Capsicum. The method of any one of to , wherein the CS amino acid sequence is derived from a plant of the genus.6Capsicum. The method of claim 5 , wherein the genus plant is a ghost chili plant.7. The method of any one of to claim 5 , wherein the CS comprises an amino acid sequence having at least 90% identity to SEQ ID NO: 1.8. The method of claim 7 , wherein the CS comprises the amino ...

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Номер записи: 70

09-01-2020 дата публикации

ACYL-ACP REDUCTASE WITH IMPROVED PROPERTIES

Номер: US20200010813A1

Автор: Gano Jacob, Rude Mathew, Schirmer Andreas, TRINH Na

Принадлежит:

The disclosure relates to acyl-ACP reductase (AAR) enzyme variants that result in improved fatty aldehyde and fatty alcohol production when expressed in recombinant host cells. The disclosure further relates to methods of making and using such AAR variants for the production of fatty alcohol compositions having particular characteristics. 182.-. (canceled)83. A variant acyl-ACP reductase (AAR) polypeptide comprising at least 90% sequence identity to the amino acid sequence of SEQ ID NO: 80 , wherein said variant AAR polypeptide comprises a mutation at amino acid position selected from the group consisting of Q40V , G52V , G273E , K303G , H340P , L344A , L344D , L344S , L344T , A345R , V346P , V346G , and A345* , and wherein said AAR polypeptide catalyzes the conversion of an acyl-ACP to a fatty aldehyde.84. The variant AAR polypeptide of claim 83 , wherein expression of the variant AAR polypeptide in a recombinant host cell results in a higher titer of a fatty aldehyde or fatty alcohol composition as compared to a titer of a fatty aldehyde or fatty alcohol composition produced by expression of a wild type AAR polypeptide in a corresponding wild type host cell.85. The variant AAR polypeptide of claim 83 , wherein said fatty alcohol composition is a C12 claim 83 , C14 or C16 fatty alcohol composition claim 83 , or a combination thereof.86. A recombinant host cell expressing the variant AAR polypeptide of .87. The recombinant host cell of claim 86 , wherein the recombinant host cell produces a fatty aldehyde or fatty alcohol composition with a titer that is at least 10% greater claim 86 , at least 15% greater claim 86 , at least 20% greater claim 86 , at least 25% greater claim 86 , or at least 30% greater than the titer of a fatty aldehyde or alcohol composition produced by a host cell expressing a corresponding wild type AAR polypeptide claim 86 , when cultured in medium containing a carbon source under conditions effective to express the variant AAR polypeptide.88. ...

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Номер записи: 71

09-01-2020 дата публикации

METHOD FOR THE PRODUCTION OF ISOAMYL ALCOHOL

Номер: US20200010858A1

Автор: Allard Mathieu, Anissimova Maria

Принадлежит: Global Bioenergies

Described is a method for the production isoamyl alcohol (3-methylbutan-1-ol) comprising the enzymatic conversion of 3-methylbutyryl-CoA (isovaleryl-CoA) into isoamyl alcohol comprising: (a) two enzymatic steps comprising (i) first the enzymatic conversion of 3-methylbutyryl-CoA into 3-methylbutyraldehyde (3-methylbutanal or isovaleraldehyde); and (ii) then enzymatically converting the thus obtained 3-methylbutyraldehyde into said isoamyl alcohol; or (b) a single enzymatic reaction in which 3-methylbutyryl-CoA is directly converted into isoamyl alcohol by making use of an alcohol-forming short chain acyl-CoA dehydrogenase/fatty acyl-CoA reductase or an alcohol-forming fatty acyl-CoA reductase (long-chain acyl-CoA:NADPH reductase) (EC 1.2.1.84). Further, described is the above method wherein the 3-methylbutyryl-CoA can be provided by the enzymatic conversion of 3-methylcrotonyl-CoA into said 3-methylbutyryl-CoA. It is also described that the thus obtained isoamyl alcohol can be further enzymatically converted into 3-methylbutyl acetate (isoamyl acetate) as described herein. Described are also recombinant organisms or microorganisms which are capable of performing the above enzymatic conversions. Furthermore, described are uses of enzymes and enzyme combinations which allow the above enzymatic conversions. 1. A method for the production of isoamyl alcohol (3-methylbutan-1-ol) comprising the enzymatic conversion of 3-methylbutyryl-CoA into isoamyl alcohol comprising: (i) first the enzymatic conversion of 3-methylbutyryl-CoA into 3-methylbutyraldehyde; and', '(ii) then enzymatically converting the thus obtained 3-methylbutyraldehyde into said isoamyl alcohol; or, '(a) two enzymatic steps comprising'}(b) a single enzymatic reaction in which 3-methylbutyryl-CoA is directly converted into isoamyl alcohol by making use of an alcohol-forming short chain acyl-CoA dehydrogenase/fatty acyl-CoA reductase or an alcohol-forming fatty acyl-CoA reductase (long-chain acyl-CoA:NADPH ...

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Номер записи: 72

03-02-2022 дата публикации

Provision of malonyl-coa in coryneform bacteria and method for producing polyphenoles and polyketides with coryneform bacteria

Номер: US20220033786A1

Автор: Jan Marienhagen, Lars Milke, Michael Bott, Nicolai Kallscheuer

Принадлежит: FORSCHUNGSZENTRUM JUELICH GMBH

A coryneform bacteria cell with an increased provision of Malonyl-CoA compared to its archetype, wherein the regulation and/or expression of one or more of genes fasB, gltA, accBC and accD1, and/or the functionality of the enzyme encoded by each gene is modified in a targeted manner. The cell may have one or more targeted modifications, including reduced or eliminated functionality of the fatty acid synthase FasB, mutation or partial or complete deletion of the fatty acid synthase encoding gene fasB, and/or reduced functionality of the promoter operatively linked to the citrate synthase gene gtIA, among other targeted modifications.

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Номер записи: 73

15-01-2015 дата публикации

KETOREDUCTASE POLYPEPTIDES FOR THE REDUCTION OF ACETOPHENONES

Номер: US20150017695A1

Автор: Ching Charlene, Gruber John M., Huisman Gjalt W., Jenne Stephane J., Krebber Anke, Liang Jack, Mundorff Emily

Принадлежит:

The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds. 1Lactobacillus. A method for stereoselectively reducing 2 ,6-dichloro-3′-fluoroacetophenone substrate , optionally substituted at one or more positions selected from the group consisting of 3′ , 4′ , and 5′ , to the corresponding substituted (S)-1-phenethanol , which comprises contacting the substrate with an engineered ketoreductase polypeptide under reaction conditions suitable for stereoselectively reducing or converting the substrate to the corresponding substituted (S)-1-phenethanol product , wherein the engineered ketoreductase polypeptide is derived from a wild-type ketoreductase comprising an amino acid sequence at least 90% identical to the reference sequence of SEQ ID NO: 2 , 4 , or 98; and wherein said ketoreductase is capable of stereoselectively reducing 2 ,6-dichloro-3′-fluoroacetophenone to (S)-1-(2 ,6-dichloro-3-fluorophenyl) ethanol.2. The method of claim 1 , wherein the (S)-1-(2 claim 1 ,6-dichloro-3-fluorophenyl) ethanol is formed in greater than 90% stereomeric excess.3. The method of claim 1 , wherein the (S)-1-(2 claim 1 ,6-dichloro-3-fluorophenyl) ethanol is formed in greater than 99% stereomeric excess.4. The method of claim 1 , wherein at least 95% of the substrate is reduced to the product in less than 24 hours when the method is conducted with at least 200 g/L of substrate and with less than 2 g/L of the polypeptide.5. The method of claim 1 , wherein the method is carried out with whole cells that express the engineered ketoreductase polypeptide claim 1 , or an extract or lysate of such cells.6. The method of claim 1 , wherein the ...

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Номер записи: 74

21-01-2016 дата публикации

NOVEL BACTERIA AND METHODS OF USE THEREOF

Номер: US20160017276A1

Автор: Heijstra Bjorn Daniel, Kern Evgenia, Koepke Michael, Liew FungMin, Segovia Simon

Принадлежит:

The invention provides a novel bacterium with improved properties, including improved ethanol production, ethanol productivity, CO uptake, specific growth rate, ethanol to acetate ratio, and alcohol tolerance. The bacterium may be derived from and/or may comprise at least one DNA or amino acid sequence selected from SEQ ID NOs: 1, 3, 6, 8, 10, 12, 14, and 16. In one embodiment, the bacterium is deposited under DSMZ accession number DSM23693 or a bacterium derived therefrom. 1Clostridium autoethanogenum. A bacterium derived from wherein the bacterium comprises least one DNA or amino acid sequence selected from SEQ ID NOs: 1 , 3 , 6 , 8 , 10 , 12 , 14 , and 16.2. The bacterium of claim 1 , wherein the bacterium is a bacterium deposited under DSMZ accession number DSM23693 or a bacterium derived therefrom.3Clostridium autoethanogenumClostridium autoethanogenum.. The bacterium of claim 1 , wherein the bacterium is derived from deposited under DSMZ accession number DSM19630 or naturally occurring4. The bacterium of claim 1 , wherein the bacterium ferments a gaseous substrate to produce one or more products.5. The bacterium of claim 4 , wherein the gaseous substrate comprises one or more of CO claim 4 , CO claim 4 , and H.6. The bacterium of claim 4 , wherein the products comprise one or more of alcohol and acetate.7. The bacterium of claim 6 , wherein the alcohol is ethanol.8. A method of producing a product comprising culturing the bacterium of in the presence of a gaseous substrate whereby the bacterium produces one or more products.9. The method of claim 8 , wherein the bacterium is a bacterium deposited under DSMZ accession number DSM23693 or a bacterium derived therefrom.10Clostridium autoethanogenumClostridium autoethanogenum.. The method of claim 8 , wherein the bacterium is derived from deposited under DSMZ accession number DSM19630 or naturally occurring11. The method of claim 8 , wherein the gaseous substrate comprises one or more of CO claim 8 , CO claim 8 , ...

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Номер записи: 75

21-01-2016 дата публикации

Xylose isomerases and their uses

Номер: US20160017310A1

Автор: Adam Martin Burja, David Neal Nunn, Jr., Jon Peter Flash BARTNEK, LING Li, Peter Luginbuhl

Принадлежит: BP Corp North America Inc

This disclosure relates to novel xylose isomerases and their uses, particularly in fermentation processes that employ xylose-containing media.

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Номер записи: 76

16-01-2020 дата публикации

SYNTHETIC METHYLOTROPHS AND USES THEREOF

Номер: US20200017888A1

Автор: Antoniewicz Maciek, Bennett Robert Kyle, Gonzalez Jacqueline, Papoutsaki Eleftherios T., Whitaker William Brian

Принадлежит: University of Delaware

The present invention provides a method for increasing production of a metabolite by a non-naturally occurring methylotroph, comprising growing the non-naturally occurring methylotroph in a medium comprising methanol. Expression of one or more native genes in the non-naturally occurring methylotroph is changed. Also provided are the non-naturally occurring methylotroph and preparation thereof. 1. A method for increasing production of a metabolite by a non-naturally occurring methylotroph , comprising growing the non-naturally occurring methylotroph in a medium comprising methanol , wherein expression of one or more native genes in the non-naturally occurring methylotroph is changed.2. The method of claim 1 , wherein the one or more native genes comprise 2 claim 1 ,3-bisphosphoglycerate-dependent phosphoglycerate mutase gene (gpmA) claim 1 , 2 claim 1 ,3-bisphosphoglycerate-independent phosphoglycerate mutase gene (gpmM) claim 1 , 2-dehydro-3-deoxy-D-gluconate 6-phosphate (KDPG) aldolase gene (eda) claim 1 , 6-phosphogluconate dehydrogenase gene (gnd) claim 1 , aminomethyltransferase gene (gcvT) claim 1 , bifunctional (p)ppGpp synthetase II/guanosine-3′ claim 1 ,5′-bis pyrophosphate 3′-pyrophosphohydrolase gene (spoT) claim 1 , enolase gene (eno) claim 1 , fructose-1 claim 1 ,6-bisphosphatase 1 class 2 gene (glpX) claim 1 , fructose-1 claim 1 ,6-bisphosphatase class 1 gene (fbp) claim 1 , GDP pyrophosphokinase/GTP pyrophosphokinase gene (relA) claim 1 , glucose-6-phosphate isomerase gene (pgi) claim 1 , glyceraldehyde-3-phosphate dehydrogenase gene (gapA) claim 1 , glyceraldehyde-3-phosphate dehydrogenase gene (gapC) claim 1 , glycine cleavage system H protein gene (gcvH) claim 1 , glycine decarboxylase gene (gcvP) claim 1 , HTH-type transcriptional regulator GntR gene (gntR) claim 1 , leucine-responsive regulatory protein gene (lrp) claim 1 , methylglyoxal synthase gene (mgsA) claim 1 , phosphogluconate dehydratase gene (edd) claim 1 , phosphoglycerate dehydrogenase ...

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Номер записи: 77

16-01-2020 дата публикации

PRODUCTION OF PRODUCTS FROM BIOMASS

Номер: US20200017894A1

Автор: Masterman Thomas Craig, Medoff Marshall, MOON Jaewoong, YOSHIDA Aiichiro

Принадлежит:

The processes disclosed herein include saccharifying cellulosic and/or lignocellulosic biomass and fermenting the sugars to produce a sugar alcohol. 126.-. (canceled)27. A method for making sugar alcohols , the method comprising:combining cellulosic or lignocellulosic biomass that contains one or more sugars with a microorganism, the recalcitrance of the biomass having been reduced by bombardment with electrons; and{'i': 'Moniliella.', 'utilizing jet mixing agitating while maintaining the microorganism-biomass combination under conditions that enable the microorganism to ferment at least one of the sugars to a sugar alcohol, wherein said conditions comprise utilizing a jet mixer while sparging air into the combination at 0.3 to 1 VVM to provide a dissolved oxygen level above 10%, and wherein said sugar alcohol is erythritol and the microorganism is a species of'}28. The method of claim 27 , further comprising saccharifying the cellulosic or lignocellulosic biomass.29M. megachiliensis.. The method of claim 27 , wherein the microorganism is30. The method of claim 27 , wherein the cellulosic or lignocellulosic biomass is selected from the group consisting of: paper claim 27 , paper products claim 27 , paper waste claim 27 , paper pulp claim 27 , pigmented papers claim 27 , loaded papers claim 27 , coated papers claim 27 , filled papers claim 27 , magazines claim 27 , printed matter claim 27 , printer paper claim 27 , polycoated paper claim 27 , card stock claim 27 , cardboard claim 27 , paperboard claim 27 , cotton claim 27 , wood claim 27 , particle board claim 27 , forestry wastes claim 27 , sawdust claim 27 , aspen wood claim 27 , wood chips claim 27 , grasses claim 27 , switchgrass claim 27 , miscanthus claim 27 , cord grass claim 27 , reed canary grass claim 27 , grain residues claim 27 , rice hulls claim 27 , oat hulls claim 27 , wheat chaff claim 27 , barley hulls claim 27 , agricultural waste claim 27 , silage claim 27 , canola straw claim 27 , wheat straw ...

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Номер записи: 78

21-01-2021 дата публикации

METHOD FOR OBTAINING LOW ETHANOL-PRODUCING YEAST STRAINS, YEAST STRAINS OBTAINED THEREFROM AND THEIR USE

Номер: US20210017613A1

Автор: Dequin Sylvie, Noble Jessica, Ortiz-Julien Anne, Tilloy Valentin

Принадлежит:

The present disclosure concerns a process for obtaining a variant yeast strain capable of producing less ethanol in an alcoholic fermentation process than its corresponding ancestral strain. The variant yeast strain is obtained by culturing the ancestral strain in the presence of increasing concentrations of a salt capable of causing an hyperosmotic stress to the ancestral yeast strain. The present disclosure also concerns variant yeast strain obtained from this process (for example the variant yeast strain deposited at Institut Pasteur, on Jan. 9, 2014, under accession number CNCM I-4832, the variant yeast strain deposited at Institut Pasteur, on Oct. 18, 2012 under accession number CNCM I-4684, the variant yeast strain deposited at Institut Pasteur, on Oct. 18, 2012 under accession number CNCM I-4685 and/or the variant yeast strain deposited at Institut Pasteur on Jan. 28, 2015 under accession number CNCM I-4952) as well as processes using the variant yeast strain (wine fermentation for example). 2. The process of claim 1 , wherein the concentration of the salt in the first culture medium is between about 1.25 M and less than about 2.4 M and/or in the second culture medium is at least about 2.4 M.3. The process of or claim 1 , further comprising claim 1 , at step a) claim 1 , increasing the salt concentration weekly or monthly.4. The process of any one of to claim 1 , wherein the first culture medium comprises glucose and the process further comprises claim 1 , at step a) claim 1 , culturing the ancestral yeast strain in the first culture medium in decreasing glucose concentrations claim 1 , wherein the concentration of glucose is preferably decreased weekly or monthly and wherein the concentration of glucose in the first culture medium is more preferably between about 14.0% and about 8.0% (w/v) with respect to the total volume of the first culture medium.5. The process of any one of to claim 1 , wherein the second culture medium comprises glucose and the process ...

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Номер записи: 79

18-01-2018 дата публикации

Compositions Comprising A Polypeptide Having Cellulolytic Enhancing Activity And A Quinone Compound And Uses Thereof

Номер: US20180019412A1

Автор: Feng Xu, Jason Quinlan, Matthew Sweeney

Принадлежит: Novozymes Inc

The present invention relates to compositions comprising: a polypeptide having cellulolytic enhancing activity and a quinone compound. The present invention also relates to methods of using the compositions.

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Номер записи: 80

17-04-2014 дата публикации

Enhanced Fermentation From Pretreatment Products

Номер: US20140106418A1

Автор: Benjamin STAUBER, Carl P. FELICE, Sarad Parekh

Принадлежит: Sweetwater Energy Inc

Provided are methods and compositions for higher yields through the addition of pretreatment/hydrolysis extracts in the fermentation process. These fractions increase the rate of growth for biocatalysts and result in improved composition of fermentation end products.

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Номер записи: 81

17-04-2014 дата публикации

Diffusion-based method for obtaining volatile hydrocarbons produced by photosynthetic microorganisms in two-phase bioreactors

Номер: US20140106422A1

Автор: Anastasios Melis, Fiona Bentley, Hsu-Ching Chen Wintz

Принадлежит: UNIVERSITY OF CALIFORNIA

This invention relates to methods of obtaining volatile hydrocarbons produced by photosynthetic microorganisms using a two-phase gaseous/aqueous bioreactor.

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Номер записи: 82

17-04-2014 дата публикации

Method of Preparing Piceatannol Using Bacterial Cytochrome P450 and Composition Therefor

Номер: US20140106423A1

Автор: Kim Dong Hyun, YUN Chul Ho

Принадлежит: GLO BIOTECH

Provided is a method of preparing piceatannol, and more particularly, to a method of preparing piceatannol from resveratrol using bacterial cytochrome P450 BM3 (CYP102A1) or mutants thereof, and a composition and a kit therefor. 1B. megaterium.. A method of preparing piceatannol , the method comprising reacting at least one enzyme selected from a group consisting of wild-type CYP102A1 and mutants of CYP102A1 with resveratrol wherein CYP102A1 is a cytochrome P450 from2. The method of claim 1 , further comprising adding an NADPH-generating system.3. The method of claim 2 , wherein the NADPH-generating system comprises glucose 6-phosphate claim 2 , NADP+ claim 2 , and yeast glucose 6-phosphate.4. The method of claim 1 , wherein the resveratrol is trans-resveratrol.5. The method of claim 1 , wherein the mutants of CYP102A1 are prepared by substituting one or more amino acids of SEQ ID NO:16 from the group consisting of:{'sup': 'th', 'substituting 47amino acid arginine (R) of wild-type CYP102A1 with one amino acid selected from a group consisting of alanine, valine, leucine, isoleucine, proline, methionine, phenylalanine, and tryptophan,'}{'sup': 'st', 'substituting 51amino acid tyrosine (Y) of wild-type CYP102A1 with one amino acid selected from a group consisting of phenylalanine, alanine, valine, leucine, isoleucine, proline, methionine, tryptophan,'}{'sup': 'th', 'substituting 64amino acid glutamic acid (E) of wild-type CYP102A1 with one amino acid selected from a group consisting of glycine, serine, threonine, cysteine, tyrosine, asparagine, and glutamine,'}{'sup': 'th', 'substituting 74amino acid alanine (A) of wild-type CYP102A1 with one amino acid selected from a group consisting of glycine, serine, threonine, cysteine, tyrosine, asparagine, and glutamine,'}{'sup': 'st', 'substituting 81amino acid phenylalanine (F) of wild-type CYP102A1 with one amino acid selected from a group consisting of alanine, valine, leucine, isoleucine, proline, methionine, and ...

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Номер записи: 83

28-01-2016 дата публикации

Electro-autotrophic synthesis of higher alcohols

Номер: US20160024533A1

Автор: James C. Liao, Kwang Myung Cho

Принадлежит: UNIVERSITY OF CALIFORNIA

The disclosure provides a process that converts CO 2 to higher alcohols (e.g. isobutanol) using electricity as the energy source. This process stores electricity (e.g. from solar energy, nuclear energy, and the like) in liquid fuels that can be used as high octane number gasoline substitutes. Instead of deriving reducing power from photosynthesis, this process derives reducing power from electrically generated mediators, either H 2 or formate. H 2 can be derived from electrolysis of water. Formate can be generated by electrochemical reduction of CO 2 . After delivering the reducing power in the cell, formate becomes CO 2 and recycles back. Therefore, the biological CO 2 fixation process can occur in the dark.

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Номер записи: 84

22-01-2015 дата публикации

Microorganism belonging to yarrowia genus, and oil decomposition agent and oil decomposition/removal method using same

Номер: US20150024470A1

Автор: Katsutoshi Hori, Masatake Fujioka

Принадлежит: Nagoya University NUC

The present invention addresses the problem of providing a new microorganism that is useful for efficiently decomposing oils and fats, and a use for said microorganism. According to screening results, a new Yarrowia lipolytica having a high capacity to assimilate free fatty acids was successfully obtained. Efficient decomposition of oils and fats is achieved by causing the Yarrowia lipolytica to act under conditions in which fatty acids that are hydrolysis products of oils or fats are present, or under conditions in which oils or fats are decomposed into fatty acids and glycerol.

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Номер записи: 85

25-01-2018 дата публикации

CHIMERIC ENZYMES FOR CONVERSION OF LIGNIN-DERIVED CHEMICALS

Номер: US20180023061A1

Автор: AHMED Alaa Ashraf Mahmoud, BECKHAM Gregg Tyler, EITEMAN Mark Andrew, JOHNSON Christopher W., LINGER Jeffrey G., NEIDLE Ellen Lee, TUMEN-VELASQUEZ Melissa

Принадлежит:

Disclosed herein are enzymes useful for the dealkylation of aromatic substrates, including the conversion of guaiacol or guaethol to catechol. Methods of converting aromatic substrates found in lignin-based feedstocks such as pyrolysis oil into products such as catechol are also disclosed. Also presented herein are methods for rapidly evolving and optimizing genetic regions. 1. An isolated DNA molecule encoding a chimeric enzyme comprising a cytochrome P450 polypeptide and a catechol 1 ,2-dioxygenase polypeptide.2Amycolatopsis. The isolated DNA molecule of claim 1 , wherein the chimeric enzyme comprises at least 90% of the amino acids of the cytochrome P450 polypeptide GcoA from sp. ATCC 39116.3Acinetobacter baylyi. The isolated DNA molecule of claim 2 , wherein the chimeric enzyme further comprises at least 90% of the amino acids of the catechol 1 claim 2 ,2-dioxygenase CatA from ADP1.4. The isolated DNA molecule of claim 1 , wherein the chimeric enzyme has an amino acid sequence at least 90% identical to SEQ ID NO:2 or SEQ ID NO:10.5. The isolated DNA molecule of claim 1 , wherein the chimeric enzyme has the amino acid sequence of SEQ ID NO:2 or SEQ ID NO:10.6. The isolated DNA molecule of claim 1 , further comprising an exogenous promoter operably linked to the DNA molecule.7. An expression vector comprising the DNA molecule of .8. A host cell that expresses a recombinant polypeptide encoded by the DNA molecule of .9PseudomonasAcinetobacter.. The host cell of claim 8 , wherein the cell is from a strain of or10P. putida.. The host cell of claim 9 , wherein the cell is11. An isolated chimeric enzyme polypeptide encoded by the DNA molecule of .12. A method for removing an alkyl group from an aromatic substrate claim 1 , comprising contacting a material containing the aromatic substrate with a chimeric enzyme comprising a cytochrome P450 polypeptide and a catechol 1 claim 1 ,2-dioxygenase polypeptide to generate a dealkylation product.13. The method of claim 12 , ...

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Номер записи: 86

25-01-2018 дата публикации

ALDOLASE, ALDOLASE MUTANT, AND METHOD AND COMPOSITION FOR PRODUCING TAGATOSE BY USING SAME

Номер: US20180023073A1

Автор: HONG Seung-hye, Lee Seon-Hwa, OH Deok-Kun

Принадлежит:

There are provided aldolase, an aldolase mutant, a method for producing tagatose, and a composition for producing tagatose using the same. The technical feature of the present invention is environment-friendly due to the use of an enzyme acquired from microorganisms, requires only a simple process of enzyme-immobilization, uses a low-cost substrate compared with that of a conventional method for producing tagatose, and has a remarkably high yield, thereby greatly reducing production cost while maximizing production effect. 1. A method of producing tagatose from fructose , the method comprising:reacting fructose 6-phosphate with tagatose 6-phosphate epimerase or a mutant thereof to obtain tagatose 6-phosphate; andconverting the tagatose 6-phosphate to tagatose.2. The method of claim 1 , wherein the tagatose 6-phosphate epimerase catalyzes the conversion of fructose 6-phosphate to tagatose-6-phosphate.3. The method of claim 1 , wherein the tagatose 6-phosphate epimerase is SEQ ID NOS: 1.4. The method of claim 1 , wherein the tagatose 6-phosphate epimerase is SEQ ID NOS: 2.5. The method of claim 1 , wherein the tagatose 6-phosphate epimerase is SEQ ID NOS: 3.6. The method of claim 1 , wherein the tagatose 6-phosphate epimerase is SEQ ID NOS: 4.7. The method of claim 1 , wherein the mutant comprises at least one amino acid substitution of SEQ ID NO: 1 selected from the group consisting of:i) a substitution of arginine residue with glutamine at a position corresponding to position 332,ii) a substitution of glutamine residue with alanine at a position corresponding to position 314,iii) a substitution of histidine residue with alanine at a position corresponding to position 227, andiv) a substitution of serine residue with alanine at a position corresponding to position 62.8. The method of claim 1 , wherein the fructose 6-phosphate is obtained by treating fructose or a fructose-containing material with hexokinase.9. The method of claim 1 , wherein the tagatose is obtained ...

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Номер записи: 87

25-01-2018 дата публикации

MICROORGANISMS AND METHODS FOR THE CO-PRODUCTION OF ETHYLENE GLYCOL AND THREE CARBON COMPOUNDS

Номер: US20180023101A1

Автор: KOCH Daniel Johannes, Lopes Mateus Schreiner, Parizzi Lucas Pedersen, Zeidler Ane Fernanda Beraldi

Принадлежит:

The present application relates to recombinant microorganisms useful in the biosynthesis of monoethylene glycol (MEG) and one or more three-carbon compounds such as acetone, isopropanol or propene. The MEG and one or more three-carbon compounds described herein are useful as starting material for production of other compounds or as end products for industrial and household use. The application further relates to recombinant microorganisms co-expressing a C2 branch pathway and a C3 branch pathway for the production of MEG and one or more three-carbon compounds. Also provided are methods of producing MEG and one or more three-carbon compounds using the recombinant microorganisms, as well as compositions comprising the recombinant microorganisms and/or optionally the products MEG and one or more three-carbon compounds. 2. The recombinant microorganism of claim 1 , wherein the recombinant microorganism further comprises at least one endogenous or exogenous nucleic acid molecule encoding a secondary alcohol dehydrogenase that catalyzes the conversion of acetone to isopropanol.3. The recombinant microorganism of claim 1 , wherein the recombinant microorganism further comprises: at least one endogenous or exogenous nucleic acid molecule encoding a secondary alcohol dehydrogenase that catalyzes the conversion of acetone to isopropanol; and at least one endogenous or exogenous nucleic acid molecule encoding a dehydratase that catalyzes the conversion of isopropanol to propene.4. The recombinant microorganism of claim 1 , wherein the recombinant microorganism further comprises one or more modifications selected from the group consisting of:(a) a deletion, insertion, or loss of function mutation in a gene encoding a D-xylose isomerase that catalyzes the conversion of D-xylose to D-xylulose;(b) a deletion, insertion, or loss of function mutation in a gene encoding a glycolaldehyde dehydrogenase that catalyzes the conversion of glycolaldehyde to glycolic acid; and(c) a deletion, ...

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Номер записи: 88

25-01-2018 дата публикации

MATERIALS AND METHODS UTILIZING BIOTIN PRODUCING MUTANT HOSTS FOR THE PRODUCTION OF 7-CARBON CHEMICALS

Номер: US20180023102A1

Автор: Cartman Stephen Thomas, FOSTER Alexander Brett, KENNEDY Jonathan

Принадлежит:

Disclosed are methods for regulating biosynthesis of at least one of pimelic acid, 7-aminoheptanoic acid, 7-hydroxyheptanoic acid, heptamethylenediamine, 7-aminohelptanol and 1,7-heptanediol (C7 building blocks) using a pathway having a pimeloyl-ACP intermediate, the method including the step of downregulating the activity of BioF. Also disclosed are recombinant hosts by fermentation in which the above methods are performed. Further disclosed are recombinant hosts for producing pimeloyl-ACP, the recombinant host including a deletion of a bioF gene. 1. A method for regulating biosynthesis of at least one C7 building block using a pathway having a pimeloyl-ACP intermediate , said method comprising downregulating the activity of a BioF enzyme.2. (canceled)3. The method of claim 1 , wherein the at least one C7 building block is chosen from pimelic acid claim 1 , 7-aminoheptanoate claim 1 , 7-hydroxyheptanoate claim 1 , heptamethylenediamine claim 1 , 7-aminoheptanol claim 1 , and 1 claim 1 ,7-heptanediol.4. The method of claim 1 , wherein:the step of downregulating the activity of a BioF enzyme comprises downregulating the expression of a BioF protein;the step of downregulating the activity of a BioF enzyme comprises downregulating the activity of a BioF protein; and/orthe step of downregulating the activity of a BioF enzyme comprises reducing the activity to zero.5. (canceled)6. The method of claim 1 , said method further comprisingoverexpressing a BioW enzyme and a CoA-specific BioF enzyme.7. The method of claim 6 , wherein:said BioW enzyme enzymatically converts pimelic acid to pimeloyl-CoA; orsaid CoA-specific BioF enzyme enzymatically converts pimeloyl-CoA to 8-amino-7-oxo-nonanoic acid.810-. (canceled)11. The method of claim 1 , wherein said pathway comprises enzymatically synthesizing a C7 aliphatic backbone from malonyl-ACP via two cycles of methyl-ester shielded carbon chain elongation claim 1 , and enzymatically forming two terminal functional groups ...

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Номер записи: 89

10-02-2022 дата публикации

Process And Microorganism For Synthesis Of Adipic Acid From Carboxylic Acids

Номер: US20220041998A1

Автор: JOO Jeong Chan, Khusnutdinova Anna, Mahadevan Radhakrishnan, Yakunin Alexander

Принадлежит:

A method for biosynthesis of polymer precursors, including, adipic acid, 1,6-hexanediol, 6-hydroxyhexanoic and 6-aminocaproic acids from carboxylic acids is provided. A method for biosynthesis of adipic acid from six-carbon dicarboxylic acids having α, β-enoate reductase activity by treatment with an enzyme is provided. The biocatalytic conversion of aliphatic and hydroxycarboxylic acids to corresponding aldehydes, alcohols, and amines using novel carboxylate reductases, aldehyde reductases, and aminotransferases is described. Also provided are genetically engineered microorganisms for use in the biosynthetic processes. 1. A process for producing a bifunctional aldehyde , as a product or an intermediate , the process comprising enzymatically converting a bifunctional carboxylic acid to a corresponding bifunctional aldehyde using a polypeptide having carboxylate reductase activity , the polypeptide comprising an amino acid sequence at least 80% identical to the amino acid sequence of any one of SEQ ID NOs: 35 , 36 , 37 , 38 , or 70.2. The process of claim 1 , wherein in addition to a first carboxyl functional group claim 1 , the bifunctional carboxylic acid has an oxo- claim 1 , a hydroxy- claim 1 , an amino- claim 1 , or a second carboxyl group as a second functional group.3. The process of claim 2 , wherein there are at least three carbons between the first carboxyl functional group and the second functional group.4. The process of claim 1 , wherein the bifunctional carboxylic acid substrate is a C5-C10 dicarboxylic acid.5. The process of claim 1 , wherein:(a) the bifunctional carboxylic acid is adipic acid and the corresponding bifunctional aldehyde produced is 6-oxohexanoic acid;(b) the bifunctional carboxylic acid is 6-hydroxyhexanoic acid and the corresponding bifunctional aldehyde produced is 6-hydroxyhexanal;(c) the bifunctional carboxylic acid is 6-aminocaproic acid and the corresponding bifunctional aldehyde produced is 6-aminohexanal; or(d) the ...

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Номер записи: 90

10-02-2022 дата публикации

LIPOXYGENASE-CATALYZED PRODUCTION OF UNSATURATED C10-ALDEHYDES FROM POLYUNSATRURATED FATTY ACIDS

Номер: US20220042051A1

Автор: Belorgey Didier, Cerny Christoph, Haefliger Olivier, Han Lei, WANG Qi

Принадлежит:

Described herein are methods for the lipoxygenase (LOX)-catalyzed production of aliphatic unsaturated C-aldehyde compounds from polyunsaturated fatty acid (PUFA) sources, and the isolation and characterization of novel, preferably bifunctional LOXs from different algae sources and the identification of structurally and/or functionally related LOXs from different bacterial sources. Also described herein are the provision of enzyme mutants derived from the newly identified enzymes, and corresponding coding sequences of the enzymes, recombinant vectors, and recombinant host cells suitable for the production of such LOXs and for performing the novel production methods of aliphatic unsaturated C-aldehyde compounds. Further describes herein is the use of particular aldehydes or aldehyde mixtures as a flavor ingredient or ingredient for food or feed compositions. 4. The method of claim 1 , wherein the polypeptide comprises an amino acid sequence selected froma) SEQ ID NO: 3, 6, 9, 12 or 15;b) SEQ ID NO: 18c) SEQ ID NO: 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, or 50; d) amino acid sequences having at least 40% sequence identity to at least one of the sequences of a), b) or c) and retaining said enzymatic activity of a lipoxygenase; ande) single and multiple mutants of anyone of the polypeptides c) retaining said enzymatic activity of a lipoxygenase.5. The method of claim 1 , wherein the polypeptide comprises the enzymatic activity of a bifunctional lipoxygenase.6. The method of claim 1 , wherein the polypeptide comprises the ability of converting at least one PUFA to at least one mono- or polyunsaturated aliphatic aldehyde.7. The method of claim 6 , wherein said decadienal is selected from 2E claim 6 ,4E-decadienal and 2E claim 6 ,4Z-decadienal and mixtures thereof; and wherein said decatrienal is selected from 2E claim 6 ,4E claim 6 , 7Z-decatrienal and 2E claim 6 ,4Z claim 6 ,7Z-decatrienal and mixtures thereof.8. The method of claim 1 , wherein said ...

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Номер записи: 91

24-01-2019 дата публикации

Mutants of unspecific peroxygenase with high monooxygenase activity and uses thereof

Номер: US20190024058A1

Автор: Francisco José PLOU GASCA, Miguel Alcalde Galeote, Patricia GÓMEZ DE SANTOS, Patricia MOLINA ESPEJA

Принадлежит: Consejo Superior de Investigaciones Cientificas CSIC

The invention relates to an unspecific peroxygenase of the Agrocybe aegerita fungus, obtained by means of directed molecular evolution to facilitate the functional expression thereof in an active, soluble and stable form. The peroxygenase described in the invention shows a significant improvement in the functional expression thereof, improved monooxygenase activity and reduced peroxidase activity, in relation to the monooxygenase and peroxidase activities showed by the unspecific wild-type peroxygenase of A. aegerita . The peroxygenase of the invention is useful in chemical processes, including industrial transformations such as the selective oxyfunctionalisation of carbon-hydrogen bonds of various organic compounds.

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Номер записи: 92

23-01-2020 дата публикации

ENZYME FOR SYNTHESIZING HYDROXYL ACETALDEHYDE AND/OR 1,3-DIHYDROXYACETONE BY CATALYZING FORMALDEHYDE, AND APPLICATIONS THEREOF

Номер: US20200024590A1

Автор: Gou Junran, Jiang Huifeng, Liu Yuwan, Lu Lina, Lu Xiaoyun, Yang Sheng, Yang Yiqun

Принадлежит:

An enzyme synthesizes hydroxyl acetaldehyde and/or 1,3-dihydroxyacetone by catalyzing formaldehyde. Site-directed mutation of benzoylformate decarboxylase (BFD) creates a mutant of the enzyme, which can polymerize the formaldehyde, A phosphoketalose (F/XPK) generates acetyl phosphoric acid from the hydroxyl acetaldehyde or 1,3-dihydroxyacetone (DHA). Combination with phosphotransacetylase (Pta) provides a route from the formaldehyde to acetyl coenzyme A in three steps. 114-. (canceled)15. A benzoylformate decarboxylase (BFD) mutant protein , which is a protein obtained by performing one or more mutations on the amino acid residues of the BFD amino acid sequence in a range of 10 Å or 8 Å or 5 Å or 3 Å away from an active center.16. The BFD mutant protein according to claim 15 , wherein: compared with the BFD amino acid sequence claim 15 , the BFD mutant protein has mutations at or merely at any one or more of the following positions: position 25 claim 15 , position 26 claim 15 , position 86 claim 15 , position 87 claim 15 , position 109 claim 15 , position 110 claim 15 , position 184 claim 15 , position 236 claim 15 , position 281 claim 15 , position 282 claim 15 , position 374 claim 15 , position 376 claim 15 , position 377 claim 15 , position 379 claim 15 , position 380 claim 15 , position 397 claim 15 , position 401 claim 15 , position 430 claim 15 , position 457 claim 15 , position 459 claim 15 , position 460.17. The BFD mutant protein according to claim 16 , wherein: compared with the BFD amino acid sequence claim 16 , the BFD mutant protein has or merely has any one or more of the following mutations: G25H claim 16 , S26T claim 16 , S26H claim 16 , S261 claim 16 , W86R claim 16 , N87T claim 16 , L109H claim 16 , L109G claim 16 , L110E claim 16 , R184H claim 16 , S236M claim 16 , H281V claim 16 , Q282F claim 16 , N374D claim 16 , N374E claim 16 , S376V claim 16 , T377G claim 16 , T377M claim 16 , T379R claim 16 , T380C claim 16 , T380Y claim 16 , F397A claim 16 ...

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Номер записи: 93

23-01-2020 дата публикации

COMPOSITIONS AND METHODS OF BIOSYNTHESIZING CAROTENOIDS AND THEIR DERIVATIVES

Номер: US20200024607A1

Автор: Wang Yechun

Принадлежит:

The present invention relates to compositions and methods of producing carotenoids and carotenoid derivatives. 1. A recombinant microorganism comprising at least one nucleic acid construct comprising:a) a nucleic acid sequence encoding a lycopene β-cyclase enzyme; andb) a nucleic acid sequence encoding a carotenoid cleavage dioxygenase enzyme;wherein the nucleic acid sequences are operably linked to one or more expression control sequence, and wherein the microorganism further comprises lycopene.2Mucor circinelloidesPhycomyces blakesleeanusErwinia herbicola.. The microorganism of claim 1 , wherein the lycopene β-cyclase is selected from a lycopene cyclase enzyme of a bifunctional lycopene cyclase/phytoene synthase encoded by carRP of claim 1 , lycopene cyclase/phytoene synthase encoded by carRA of claim 1 , and lycopene cyclase encoded by crtY from3Daucus carota.. The microorganism of claim 1 , wherein the carotenoid cleavage dioxygenase enzyme is encoded by CCD1 from4. The microorganism of claim 1 , wherein the microorganism is selected from a microorganism genetically engineered to inhibit the expression of lycopene α-cyclase and a microorganism naturally not capable of expressing lycopene α-cyclase.5Yarrowia lipolytica, Saccharomyces cerevisiaeE. coli.. The microorganism of claim 1 , wherein the microorganism is selected from claim 1 , and6. The microorganism of claim 1 , comprising β-ionone.7. The recombinant microorganism of claim 1 , wherein the nucleic acid expression construct comprises a nucleic acid sequence encoding a carotenoid cleavage dioxygenase enzyme with at least 80% identity to an amino acid sequence of SEQ ID NO: 88.8. The recombinant microorganism of claim 1 , wherein the nucleic acid expression construct comprises a nucleic acid sequence encoding a lycopene β-cyclase enzyme with at least 80% identity to an amino acid sequence encoded by a nucleic acid sequence selected from SEQ ID NO: 64 and SEQ ID NO: 67.9. A recombinant microorganism ...

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Номер записи: 94

28-01-2021 дата публикации

BIOTECHNOLOGICAL PRODUCTION OF ALCOHOLS AND DERIVATIVES THEREOF

Номер: US20210024967A1

Автор: Engel Philip, Gehring Christian, Haas Thomas, Pfeffer Jan Christoph, Poetter Markus, Thum Oliver

Принадлежит: EVONIK OPERATIONS GMBH

A method for oxidizing an alkyl, including a) contacting the alkyl with an aqueous solution comprising a microorganism where the microorganism has a reduced fatty acid degradation capacity compared to its wild type, wherein the fatty acid degradation capacity is reduced by deletion, inhibition or inactivation of a gene encoding an enzyme involved in the β-oxidation pathway; and the microorganism expresses a recombinant alkane oxidase, and b) contacting the aqueous solution from a) with a water-immiscible organic solvent. 1: A method for oxidizing an alkyl , comprisinga) contacting the alkyl with an aqueous solution comprising a microorganism where the microorganism has a reduced fatty acid degradation capacity compared to its wild type, wherein the fatty acid degradation capacity is reduced by deletion, inhibition or inactivation of a gene encoding an enzyme involved in the β-oxidation pathway; and the microorganism expresses a recombinant alkane oxidase, andb) contacting the aqueous solution from a) with a water-immiscible organic solvent,{'sub': 2', 'x', '2', '2', '3', '3, 'sup': 1', '1, 'wherein the alkyl is a compound represented by the formula H—(CH)—R, wherein x is at least 8, and R is selected from the group comprising —OH, —COH, —COOH, —COOR, —NH, —NO, —CN, —OPOH, —SOH and —H wherein Ris methyl or ethyl, and'}{'i': 'Pseudomonas putida', 'wherein the expressed recombinant alkane oxidase is AlkB from GPo1 comprising the sequence of SEQ ID NO: 1 or a variant thereof having at least 90% identity to SEQ ID NO: 1, and'} a fatty acid importer comprising the sequence of SEQ ID NO: 2 or a variant thereof having at least 90% identity to SEQ ID NO: 2,', 'a fatty acid-CoA ligase comprising the sequence of SEQ ID NO: 3 or a variant thereof having at least 90% identity to SEQ ID NO: 3,', 'an acyl-CoA dehydrogenase comprising the sequence of SEQ ID NO: 4 or a variant thereof having at least 90% identity to SEQ ID NO: 4,', 'an enoyl-CoA hydratase comprising the sequence of ...

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Номер записи: 95

24-04-2014 дата публикации

Systems and Methods for Cultivating, Harvesting and Processing Biomass

Номер: US20140113331A1

Автор: Dennis Dwayne Yancey, JR.

Принадлежит: Coastal Waters Biotechnology Group LLC

Combining controlled open-ocean iron enrichment with a system for collecting the ensuing biological growth can lead to a fundamental shift towards using marine biomass feedstock for large-scale global biodiesel production. The literature review reveals that open-ocean enrichment effectively reduces both the atmospheric carbon dioxide partial pressure and ocean acidity. A semi-closed ocean system is provided that allows for the efficient cultivation and harvesting of a high tonnage biomass feedstock generated by iron fertilization. The concept methodically capitalizes on the ocean's free nutrients, kinetic/potential energy, and expansive surface area to ensure that the mass, energy, and cost balance equations favor our system while taking care to preserve the ocean's ecosystem. The system is modular, portable, easily scalable system, and minimizes waste. In addition to the above benefits, our concept allows continued adherence to the NEPA and London Protocol by culling the biomass produced by fixing carbon dioxide and limiting iron exposure to the vessel's interior.

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Номер записи: 96

24-04-2014 дата публикации

Abe fermentation method comprising product absorption through isophoron

Номер: US20140113341A1

Автор: Franz Ulrich Becker, Gerda Grund, Joerg-Joachim Nitz, Patrick STIER

Принадлежит: EVONIK DEGUSSA GmbH

The invention relates to a method comprising the following method steps: A) providing an aqueous solution comprising microorganisms producing low-molecular, organic compounds; B) introducing at least one gas or gas mixture into the aqueous solution; C) recovering the gas flow through a compound comprising an isophoron; and optionally D) separating the low-molecular, organic compound from the composition comprising the isophoron.

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Номер записи: 97

29-01-2015 дата публикации

TRANSFORMED CELLS THAT FERMENT PENTOSE SUGARS AND METHODS OF THEIR USE

Номер: US20150031076A1

Автор: Harhangi Harry Ramanoedj, OP DEN CAMP Hubertus Johannes Marie, Pronk Jacobus Thomas, Van Der Drift Christiaan

Принадлежит:

The present invention relates to host cells transformed with a nucleic acid sequence encoding a eukaryotic xylose isomerase obtainable from an anaerobic fungus. When expressed, the sequence encoding the xylose isomerase confers to the host cell the ability to convert xylose to xylulose which may be further metabolized by the host cell. Thus, the host cell is capable of growth on xylose as carbon source. The host cell preferably is a eukaryotic microorganism such as a yeast or a filamentous fungus. The invention further relates to processes for the production of fermentation products such as ethanol, in which a host cell of the invention uses xylose for growth and for the production of the fermentation product. The invention further relates to nucleic acid sequences encoding eukaryotic xylose isomerases and xylulose kinases as obtainable from anaerobic fungi. 1. A eukaryotic host cell transformed with a nucleic acid construct comprising a nucleotide sequence encoding a xylose isomerase (XI) enzyme , wherein , expression of the nucleic acid construct in the host cell confers on the host cell the ability to isomerize xylose to xylulose.2. The transformed host cell according to claim 1 , wherein the nucleotide sequence is selected from the group consisting of:(a) a nucleotide sequence encoding a polypeptide comprising an amino acid sequence that has at least 40% sequence identity with the amino acid sequence of SEQ ID NO:1;(b) a nucleotide sequence that has at least 40% sequence identity with the nucleotide sequence of SEQ ID NO:2;(c) a nucleotide sequence the complementary strand of which hybridizes to the nucleic acid sequence of (a) or (b); and(d) a nucleotide sequence which differs from the sequence of (c) due to degeneracy of the genetic code.3. The transformed host cell according to claim 2 , wherein the host cell is a yeast cell.4Saccharomyces, Kluyveromyces, Candida, Pichia, Schizosaccharomyces, Hansenula, Kloeckera, Schwanniomyces,Yarrowia.. A transformed host ...

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Номер записи: 98

29-01-2015 дата публикации

Enone reductases

Номер: US20150031095A1

Автор: Christopher K. Savile, Gjalt W. Huisman, Vesna Mitchell, Xiyun Zhang

Принадлежит: Codexis Inc

The disclosure relates to engineered enone reductase polypeptides having improved properties, polynucleotides encoding the engineered polypeptides, related vectors, host cells, and methods for making the engineered enone reductase polypeptides. The disclosure also provides methods of using the engineered enone reductase polypeptides for chemical transformations.

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Номер записи: 99

29-01-2015 дата публикации

Pesticidal flavobacterium strain and bioactive compositions metabolites and uses

Номер: US20150031534A1

Автор: Ana-Lucia Cordova-Kreylos, Lijuan XING, Margarita RODRIGUEZ, Marja Koivunen, Pamela Marrone, Ratnakar Asolkar

Принадлежит: Marrone Bio Innovations Inc

Provided is a pesticidal Flavobacterium strain and bioactive compositions and metabolites derived therefrom as well as their methods of use for controlling pests.

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Номер записи: 100