СПОСОБ КОНТРОЛЯ ФЕРМЕНТАЦИИ СО-СОДЕРЖАЩИХ СУБСТРАТОВ

СПОСОБ ФЕРМЕНТАЦИИ СОДЕРЖАЩИХ СО ГАЗООБРАЗНЫХ СУБСТРАТОВ В СРЕДЕ С НИЗКИМ СОДЕРЖАНИЕМ ФОСФАТА, ЭФФЕКТИВНЫЙ ДЛЯ СНИЖЕНИЯ ПОТРЕБЛЕНИЯ ВОДЫ

СПОСОБ КУЛЬТИВИРОВАНИЯ МИКРООРГАНИЗМОВ НА ВЫБРАННОМ НОСИТЕЛЕ

... 1. Способ культивирования бактерий на выбранном носителе, включающийуменьшение количество первого носителя с целью преобразования по меньшей мере части бактерий в споры,добавление выбранного носителя к спорам иобеспечение прорастания по меньшей мере части спор на выбранном носителе.2. Способ по п. 1, в котором выбранный носитель содержит синтез-газ.3. Способ по п. 2, в котором синтез-газ имеет молярное соотношение CO/COпо меньшей мере около 0,75.4. Способ по п. 1, в котором первый носитель содержит источник углерода, выбранный из группы, включающей дрожжевой экстракт, углеводы, спирт, аминокислоты, пептон, пептиды, белок, жирные кислоты, липид и их смеси.5. Способ по п. 1, в котором первый носитель и выбранный носитель не являются одинаковыми.6. Способ по п. 1, в котором бактериями являются ацетогенные бактерии.7. Способ по п. 6, в котором ацетогенные бактерии выбраны из группы, включающей Acetogenium kivui, Acetoanaerobium noterae, Acetobacterium woodii, Alkalibaculum bacchi CP11 (ATCC ...

Reporter systems for promoters of fermentative or metabolic pathways in bacteria

A reporter construct comprising a light-emitting reporter, such as luceiferase, operative linked to a promoter of a gene expressed in a fermentative or synthetic pathway in a cell is disclosed. The pathway may be a solventogenesis pathway, such as one producing acetone, ethanol or butanol, preferably from Clostridium, E.Coli, Z. mobilis or S. cerevisiae. The expression of the reporter correlates with the expression of the target product of the fermentative or synthetic pathway, and therefore the amount of light produced by the reporter indicates the amount of target product being produced. When the reporter is measured in real time, it provides information that can be used to regulate culture conditions and to optimize production of the target product.

Compositions comprising bacterial strains

Methods and processes for producing organic acids.

Isolation of microbial cell lines for organic acid production

Microbial cell lines suitable for industrial-scale production of organic acids and methods of making and isolating such cell lines.

Systems and methods for producing biofuels and related materials

... cells (American Type Culture Collection 700394) and all other strains of the species can ferment materials such as biomass into useful products and coproducts, such as ethanol, hydrogen and organic acids. Compositions that include are also disclosed.

Alcohol production process

The invention relates to the production of products such as alcohols and acids by microbial fermentation, particularly microbial fermentation of substrates comprising CO. It more particularly relates to methods and systems for improving efficiency of products by microbial fermentation. In particular embodiments, the invention provides a method of optimising production of desired products including the step of ascertaining the proportion of CO converted to CO2.

Continuous single vessel butanol synthesis by fermentation

The present invention describes a method for producing butanol by fermentation of carbohydrates using mixed populations of acidogenic-phase cells and solventogenic-phase cells of ...

Method for the enzymatic production of 3-hydroxy-3-methylbutyric acid from acetone and acetyl-CoA

Described is a method for the production of 3-hydroxy-3-methylbutyric acid (also referred to as beta-hydroxyisovalerate or HiV) from acetone and a compound which provides an activated acetyl group comprising the enzymatic conversion of acetone and a compound which provides an activated acetyl group into 3-hydroxy-3- methylbutyric acid. The conversion makes use of an enzyme which is capable of catalyzing the formation of a covaient bond between the carbon atom of the oxo (i.e. the C=O) group of acetone and the methyl group of the compound which provides an activated acetyl group. Preferably, the enzyme employed in the process is an enzyme with the activity of a HMG CoA synthase (EC 2.3.3.10) and/or a PksG protein and/or an enzyme with the activity of a C-C bond cleavage/condensation lyase, such as a HMG CoA lyase (EC 4.1.3.4). Also described are organisms which are able to produce 3-hydroxy-3-methylbutyc acid from acetone, a compound which provides an activated acetyl group, the use of the ...

Extraction of nitrogen from organic materials through ammonification by mixed bacterial populations

The invention provides a process for producing ammonia or ammonium from an organic material by fermenting a medium comprising organic material in the presence of a mixed bacterial population capable of ammonification, wherein the fermenting is under conditions, and for a sufficient period of time, to produce a fermentation product that comprises ammonia or ammonium. The organic material includes nitrogenous compounds suitable for conversion to ammonia or ammonium.

Method for sustaining microorganism culture in syngas fermentation process in decreased concentration or absence of various substrates

The present invention relates to methods for sustaining microorganism culture in a syngas fermentation reactor in decreased concentration or absence of various substrates comprising: adding carbon dioxide and optionally alcohol; maintaining free acetic acid concentrations; and performing the above mentioned steps within specified time.

A process for fermenting CO-containing gaseous substrates in a low phosphate medium effective for reducing water usage

A process is provided for fermenting CO-containing gaseous substrates in a low phosphate medium. The process includes blending a liquid medium that includes at least one transition metal element with a liquid medium that includes at least at least one other transition metal element and one non-metal element to provide a fermentation medium. The process is effective for preventing precipitation of one or more transition metal elements with one or more non-metal elements. The fermentation medium used in the process is prepared in a way that requires significantly lower amounts of water and reduced levels of phosphate.

PROCESS AND SYSTEM FOR OBTAINING BOTULINUM NEUROTOXIN

Rapid, animal protein free, chromatographic processes and systems for obtaining high potency, high yield botulinum neurotoxin for research, therapeutic and cosmetic use.

MUTANT STRAIN OF CLOSTRIDIUM THERMOACETICUM USEFUL FOR THE PREPARATION OF ACETIC ACID

A MUTANT STRAIN OF CLOSTRIDIUM THERMOACETICUM USEFUL FOR THE PREPARATION OF ACETIC AC ID A biologically pure mutant of C. thermoaceticum (ATCC No. 39,289) useful for the production of acetic acid by a fermentation reaction is disclosed. This mutant is capable of growing at a pH below 5.0 and shows a specific growth rate of at least 0.30 hr-1 when continuously cultured under optimum conditions.

ISOLATION OF MICROBIAL CELL LINES FOR ORGANIC ACID PRODUCTION

Microbial cell lines suitable for industrial-scale production of organic acids and methods of making and isolating such cell lines.

SYSTEMS AND METHODS FOR PRODUCING BIOFUELS AND RELATED MATERIALS

METHOD FOR SUSTAINING MICROORGANISM CULTURE IN SYNGAS FERMENTATION PROCESS IN DECREASED CONCENTRATION OR ABSENCE OF VARIOUS SUBSTRATES

The present invention relates to methods for sustaining microorganism culture in a syngas fermentation reactor in decreased concentration or absence of various substrates comprising: adding carbon dioxide and optionally alcohol; maintaining free acetic acid concentrations; and performing the above mentioned steps within specified time.

METHOD FOR TREATMENT OF DISORDERS OF THE GASTROINTESTINAL SYSTEM

There are provided novel synthetic stool preparations comprising bacteria isolated from a fecal sample from a healthy donor. The synthetic stool preparations are used for treating disorders of the gastrointestinal tract, including dysbiosis, Clostridium difficile infection and recurrent Clostridium difficile infection, prevention of recurrence of Clostridium difficile infection, treatment of Crohn's disease, ulcerative colitis, irritable bowel syndrome, inflammatory bowel disease, and diverticular disease, and treatment of food poisoning such as salmonella. Methods of preparation and methods of use of the synthetic stool preparations are also provided.

DSMZ 24726 FOR SECOND GENERATION BIOETHANOL PRODUCTION

The present invention relates to a novel anaerobic, extreme thermophilic, ethanol high- yielding bacterium. The invention is based on the isolation of the bacterial strain referred to herein as "DTU01", which produces ethanol as the main fermentation product, followed by acetate and lactate. The isolated organism is an extremely interesting and very promising organism for the establishment of a sustainable bioethanol production process. The invention further relates to a method for producing a fermentation product such as ethanol.

A PROCESS FOR CULTURING MICROORGANISMS ON A SELECTED SUBSTRATE

A process is provided that is effective for allowing bacteria to be cultured in a selected substrate. Bacteria are sporulated and then germinated in the presence of a selected substrate and medium.

COMPOSITIONS COMPRISING BACTERIAL STRAINS

isolamento de linhagens de células microbianas para produção de ácido orgânico

Produção biológica de ácido acético a partir de gases residuais.

Patente de Invenção: <B>PRODUçãO BIOLóGICA DE áCIDO ACéTICO A PARTIR DE GASES RESIDUAIS"<D>. é revelado um método e um equipamento para a conversão de gases residuais de processos industriais tais como refinação de petróleo, negro-de-fumo, coque, amónia e produção de metanol, em produtos úteis. O método inclui a introdução dos gases residuais em um biorreator onde eles são fermentados para vários produtos, tais como ácidos orgânicos, alcoóis H~ 2~, SCP e sais de ácidos orgânicos por bactérias anaeróbicas dentro do biorreator. Estes preciosos produtos finais são em seguida recuperados, isoladados e purificados.

Compositions comprising bacterial strains

The invention provides compositions comprising bacterial strains for treating and preventing inflammatory and autoimmune diseases.

Specific bacteria for their use as a medicament, in particular for controlling excess weight, obesity, cardiometabolic diseases and inflammatory bowel diseases

The object of the invention are compositions and medical treatment methods with an inheritable, Gram-negative, strictly anaerobic and commensal bacterium of the family Christensenellaceae belonging to an OTU (Operational Taxonomic Unit) characterized by a 16S rRNA sequence SEQ ID NO: 1 or to an OTU characterized by a 16S rRNA sequence SEQ ID NO: 2.

Thermophilic Organisms For Conversion of Lignocellulosic Biomass To Ethanol

Mutant thermophilic organisms that consume a variety of biomass derived substrates are disclosed herein. Strains of Thermoanaerobacterium saccharolyticum with acetate kinase and phosphotransacetylase expression eliminated are disclosed herein. Further, strain ALK1 has been engineered by site directed homologous recombination to knockout both acetic acid and lactic acid production. Continuous culture involving a substrate concentration challenge lead to evolution of ALK1, and formation of a more robust strain designated ALK2. Both organisms produce near theoretical ethanol yields without expressing pyruvate decarboxylase ...

SYSTEM AND METHOD OF BIOCATALYTIC CONVERSION FOR PRODUCTION OF ALCOHOLS, KETONES, AND ORGANIC ACIDS

Biocatalytic conversion systems and methods of producing and using same that have improved yields are disclosed. The systems and methods involve co-fermentation of sugars and gaseous substrates for alcohol, ketone, and/or organic acid production. The systems and methods may include biocatalytically converting at least one sugar substrate into at least one of alcohol, at least one ketone, and/or at least one organic acid. The systems and methods may further include biocatalytically converting gases that comprise CO2 and H2 to at least one alcohol and/or at least one organic acid, thereby adding extra revenue to biorefineries.

СПОСОБ И СИСТЕМА ДЛЯ ПОЛУЧЕНИЯ ПРОДУКТОВ, ВКЛЮЧАЮЩИХ СПИРТЫ И/ИЛИ КИСЛОТЫ, ПРИ МИКРОБИОЛОГИЧЕСКОЙ ФЕРМЕНТАЦИИ

Изобретения относятся к области биотехнологии, а именно к способу и системе для получения одного или более продуктов, включающих спирты и/или кислоты, с помощью микробиологической ферментации. Принимают из производственного процесса прерывистый или непостоянный поток(и) дымового или отходящего газа, содержащего СО, в резервуар-хранилище. Направляют по существу непрерывный поток(и) газа из резервуара-хранилища в биореактор, в котором имеется культура одной или более карбоксидотрофной бактерии в жидкой питательной среде. Ферментируют эту культуру с получением одного или более продуктов, включающих спирты и/или кислоты. Система для получения одного или более продуктов, включающих спирты и/или кислоты, содержит впуск для приема прерывистого или непостоянного потока(ов) дымового или отходящего газа, содержащего CO, резервуар-хранилище для приема указанного потока газа, выполненный с возможностью направления указанного потока газа в по существу непрерывном режиме в биореактор. Причем система ...

СПОСОБ ПОЛУЧЕНИЯ ВЫСШИХ СПИРТОВ

ОПРЕДЕЛЕННЫЕ МИКРОБИОЛОГИЧЕСКИЕ КОМПОЗИЦИИ

УСОВЕРШЕНСТВОВАННОЕ УЛАВЛИВАНИЕ УГЛЕРОДА ПРИ ФЕРМЕНТАЦИИ

... 1. Способ получения одного или более продуктов, которыми являются спирты и/или карбоновые кислоты, с помощью микробиологической ферментации, включающий стадии, на которыха) принимают из процесса производства стали поток(и) дымового или отходящего газа, содержащего CO;б) направляют этот поток(и) газа в биореактор, содержащий культуру одной или более карбоксидотрофных бактерий в жидкой питательной среде; ив) ферментируют эту культуру в биоректоре с получением одного или более продуктов, которыми являются спирты и/или карбоновые кислоты; где поток газа содержит 5-70 об.% CO.2. Способ по п. 1, где поток газа содержит 20-70 об.% CO.3. Способ по п. 1, где в результате ферментации получают этанол и/или ацетат.4. Способ по п. 1, где одна или более карбоксидотрофная бактерия выбрана из рода Clostridium.5. Способ по п. 1, где указанная карбоксидотрофная бактерия представляет собой Clostridium autoethanogenum, Clostridium ljungdahlii или Clostridium carboxydivorans.6. Способ по п. 1 или 5, где карбоксидотрофная ...

Clostridiumstämme, die aus substrathaltigen Gasgemischen Ethanol erzeugen

DECOMPOSING STRAW

A steady state anaerobic denitrifying consortium for application in-situ bioremediation of hydrocarbon-contaminated sites and enhanced oil recovery

Enhanced ABE fermentation with high yielding butanol tolerant Clostridium strains

A method and system for high-yield fermentation comprises using Clostridium species having tolerance of a concentration of butanol of up to 15% (150g/l or 2 mol/l) in a packed-bed bioreactor, to produce ABE (acetone butanol ethanol) solvents. The microorganisms are preferably grown on a solid support such as bone-char particles. Preferably the process yields in excess of 6g/l.h butanol. A bioreactor comprising a packed bed zone, a bed expansion zone and a particle disengagement zone (to prevent egress of a solid support) is separately claimed. Preferably the bioreactor is configured to alternate between packed bed and expanded bed modes of operation.

Microorganisms with inactivated lactate dehyrdrogenase gene (ldh) for chemical production

Deletion mutations

Compositions comprising bacterial strains

A lyophilised composition comprising a bacterial strain of the genus Erysipelatoclostridium (e.g. Clostridium ramosum), for use in treating or preventing a disease or condition mediated by interleukin-17 (IL-17) or the Th17 pathway. The composition can be used to treat or prevent cancers, asthma, arthritis and multiple sclerosis. The composition can reduce IL-17 production or reduce Th17 cell differentiation. The composition can be used in a patient with elevated IL-17 levels or Th17 cells. The composition can be used to treat or prevent uveitis. The bacterial strain can be viable and capable of colonising the intestine. The composition can comprise a microbial consortium. Further aspects of the invention are food products and vaccine compositions that comprise the lyophilised composition.

Compositions comprising bacterial strains

MUTANTER TRUNK OF CLOSTRIDIUM HISTOLYTICUM, PROCEDURES FOR ITS PRODUCTION AND USE FOR THE PRODUCTION OF CLOSTRIPAIN SUITOR KOLLAGENASE.

NOVEL ETHANOL PRODUCING BACTERIUM AND PROCESS FOR PRODUCING ETHANOL

ANAEROBE TARGETED ENZYME-MEDIATED PRODRUG THERAPY

Clostridium strains which produce ethanol from substrate-containing gases

STRAW DECOMPOSITION PROCESS

A novel ethanologenic Clostridium species, Clostridium coskatii

A novel Clostridia bacterial species (

Methods and processes for producing organic acids

A fermentation process for the production of organic acids is provided and includes, providing metabolically engineered mutant bacteria which have had one or both of and genes disrupted, adapting the mutant bacteria to increase their resistance to acids and to increase their growth rate by immobilizing the mutant bacteria while exposing the mutant bacteria to a fermentable substrate, and further exposing the adapted mutant bacteria to a fermentable substrate for a time sufficient to provide a final organic acid fermentation product concentration of greater than about 50 g/L.

Clostridium sartagoformum for the generation of biogas

Bacterial strain Clostridium histolyticum and its use

Bacterial strain ...

Extraction of nitrogen from organic materials through ammonification by mixed bacterial populations

The invention provides a process for producing ammonia or ammonium from an organic material by fermenting a medium comprising organic material in the presence of a mixed bacterial population capable of ammonification, wherein the fermenting is under conditions, and for a sufficient period of time, to produce a fermentation product that comprises ammonia or ammonium. The organic material includes nitrogenous compounds suitable for conversion to ammonia or ammonium.

SYSTEM AND PROCESS FOR INCREASING PROTEIN PRODUCT YIELD FROM BACTERIAL CELLS

Protein-rich nutrient supplements and animal feed supplements derived from an anaerobic bacterial process are generated through a myriad of cell rupturing and protein fractionation/purification processes. Bacterial fermentation systems and methods of obtaining one or more protein-containing portions from a fermentation process using carbon monoxide-containing gaseous substrates increasing protein product yield from bacterial cells are provided. The invention further provides compositions of protein-rich nutrient supplements with useful applications for intake by a variety of different animals and humans.

THERMOPHILIC ORGANISMS FOR CONVERSION OF LIGNOCELLULOSIC BIOMASS TO ETHANOL

Mutant thermophilic organisms that consume a variety of biomass derived substrates are disclosed herein. Strains of Thermoanaerobacterium saccharolyticum with acetate kinase and phosphotransacetylase expression eliminated are disclosed herein. Further, strain ALKl has been engineered by site directed homologous recombination to knockout both acetic acid and lactic acid production. Continuous culture involving a substrate concentration challenge lead to evolution of ALKl , and formation of a more robust strain designated ALK2. Both organisms produce near theoretical ethanol yields without expressing pyruvate decarboxylase ...

A STEADY STATE ANAEROBIC DENITRIFYING CONSORTIUM FOR APPLICATION IN IN-SITU BIOREMEDIATION OF HYDROCARBON-CONTAMINATED SITES AND ENHANCED OIL RECOVERY

Enriched steady state microbial consortiums for microbial enhanced oil recovery and in situ bioremediation of hydrocarbon-contaminated sites, under anaerobic denitrifying conditions, are disclosed.

CLOSTRIDIUM AUTOETHANOGENUM STRAIN AND METHODS OF USE THEREOF TO PRODUCE ETHANOL AND ACETATE

A novel class of bacteria is described which has improved efficiency in the production of ethanol by anaerobic fermentation of substrates containing carbon monoxide. The exemplified bacterium,Clostridium autoethanogenum, is capable of producing ethanol and acetate at a ratio of at least 1Ø ...

METHODS AND COMPOSITIONS FOR INCREASING TOXIN PRODUCTION

The invention provides methods and compositions (such as for example, culture media) for culturing Clostridium difficile and producing the C. difficile Toxins A and B.

PRODUCTION OF BUTANOL FROM CARBON MONOXIDE BY A RECOMBINANT MICROORGANISM

The invention relates, inter alia, to novel genetically modified microorganisms capable of using CO to produce 1-butanol and/or a precursor thereof, novel methyltranferases and nucleic acids encoding same, methods for producing genetically modified microorganisms using said novel methyltransferases, and methods of producing 1-butanol and/or a precursor thereof by microbial fermentation.

COMPOSITIONS AND METHODS RELATING TO A MUTANT CLOSTRIDIUM DIFFICILE TOXIN

In one aspect, the invention relates to an immunogenic composition that includes a mutant Clostridium difficile toxin A and/or a mutant Clostridium difficile toxin B. The mutant toxin may include a glucosyltransferase domain having at least one mutation and a cysteine protease domain having at least one mutation, relative to the corresponding wild-type C. difficile toxin. The mutant toxins may include at least one amino acid that is chemically crosslinked. In another aspect, the invention relates to methods and compositions for use in culturing Clostridium difficile and in producing C. difficile toxins.

RECOMBINANT MICROORGANISMS AND USES THEREFOR

Bacteria are genetically engineered to produce 3-hyrdoxypropionate (3-HP). The bacteria are carboxydotrophic acetogens. The bacteria produce acetyl-coA using the Wood-Ljungdahl pathway for fixing CO/C0?2#191. A malonyl-coA reductase from a bacterium that contains such an enzyme is introduced. Additionally, an acetyl-coA carboxylase may also be introduced The production of 3-HP can be improved by overproduction of acetyl-CoA carboxylase or by overproduction of biotin. This can be effected by improved promoters or higher copy number or enzymes that are catalytically more efficient.

MUTANT STRAIN OF C. ACETOBUTYLICUM AND PROCESS FOR MAKING BUTANOL

A biologically pure asporogenic mutant of Clostridium acetobutylicum is produced by growing sporogenic C. acetobutylicum ATCC 4259 and treating the parent strain with ethane methane sulfonate. The mutant which has been designated C. acetobutylicum ATCC 55025 is useful in an improved ABE fermentation process, and produces high concentrations of butanol and total solvents.

DEFINED MICROBIAL COMPOSITIONS

A method of producing higher alcohols

PROCESS AND SYSTEM FOR OBTAINING BOTULINUM NEUROTOXIN

Acidogenic clostridia and processes of using thereof for producing volatile fatty acids

Clostridium cadaveris strain and uses of the same

An isolated Clostridium cadaveris ITRI04005 and its uses are provided. The isolated Clostridium cadaveris ITRI04005 was deposited at German Collection of Microorganisms and Cell Cultures (Deutsche Sammlung von Mikroorganismen and Zellkulturen GmbH, DSMZ) under the accession number DSM 32078.

Methods and compositions for producing solvents

Described herein are methods, compositions and synthetic biology approaches for solvent production, including but not limited to butanol production. Described herein are recombinant bacteria and yeast strains which may be used in production of a solvent, including but not limited to butanol, from lignocellulosic and other plant-based feedstocks. Described herein are methods of producing solvents, including but not limited to butanol, using bacteria and yeast strains. Described herein are methods of producing organisms that display highly efficient butanol production.

HUMAN-DERIVED BACTERIA THAT INDUCE PROLIFERATION OR ACCUMULATION OF REGULATORY T CELLS

Species of human-derived bacteria belonging to the Clostridia class have been shown to induce accumulation of regulatory T cells (Treg cells) in the colon and suppress immune functions. Pharmaceutical compositions containing these bacteria can be used to prevent and treat immune-mediated diseases such as autoimmune diseases.

Method of producing higher alcohols

... wherein the first microorganism is further capable of converting the acid to the corresponding higher alcohol and the higher alcohol comprises at least 6 carbon atoms.

CONTINUOUS CULTURE FOR 1,3-PROPANEDIOL PRODUCTION USING HIGH GLYCERINE CONCENTRATION

The present invention concerns a new method for the production of 1,3-propanediol comprising culturing a microorganism on a culture medium with high glycerine content. The invention also concerns a new microorganism, or strain of microorganism, adapted for the production of 1,3-propanediol from medium comprising high glycerine content. The invention also concerns an adapted microorganism which glycerol metabolism is directed to 1,3-propanediol production, and which is allowed to grow in the presence of a high concentration of industrial glycerine. The invention also concerns a biosourced 1,3-propanediol obtained by the process thereof. Finally the invention concerns the use of the above described biosourced 1,3-propanediol as extender chain in thermoplastic polyurethane, as monomers in polytrimethylene terephtalate and as a component in cosmetics formulations.

PROCESS AND SYSTEM FOR OBTAINING BOTULINUM NEUROTOXIN

Rapid, animal protein free, chromatographic processes and systems for obtaining high potency, high yield botulinum neurotoxin for research, therapeutic and cosmetic use.

СПОСОБ ПОЛУЧЕНИЯ 3-ГИДРОКСИ-3-МЕТИЛМАСЛЯНОЙ КИСЛОТЫ ИЗ АЦЕТОНА И АЦЕТИЛ-СОА

Изобретение относится к области химического синтеза и микробиологии. Предложен способ получения 3-гидрокси-3-метилмаслянной кислоты из ацетона и соединения, которое обеспечивает активированную ацетильную группу. Также предложены соответствующие рекомбинантный организм, его применение, композиция, применение фермента с активностью HMG CoA синтазы и применение ацетона для получения 3-гидрокси-3-метилмаслянной кислоты. 7 н. и 6 з. п. ф-лы, 9 ил., 4 табл., 7 пр.

УСОВЕРШЕНСТВОВАННОЕ УЛАВЛИВАНИЕ УГЛЕРОДА ПРИ ФЕРМЕНТАЦИИ

Изобретение относится к усовершенствованию улавливания углерода и/или повышению эффективности способов, включающих микробиологическую ферментацию содержащего СО субстрат, полученный из промышленного источника. Предложен способ получения одного или более продуктов, которыми являются спирты и/или карбоновые кислоты, с помощью микробиологической ферментации потока(ов) дымового или отходящего газа, содержащего СО, из процесса производства стали. Процесс производства стали включает стадию обезуглероживания, осуществляемую в кислородном конвертере. Поток газа из процесса производства стали содержит 43-50% СО, 17-20% СO2, 2-3% Н2и 27-34% N2. Этот поток(и) газа направляют в биореактор, содержащий культуру карбоксидотрофных бактерий Clostridium autoethanogenum DSMZ 19630 в жидкой питательной среде. Ферментируют культуру с получением целевого продукта. Изобретение позволяет осуществлять ферментацию дымовых или отходящих газах, получаемых непосредственно из производственных процессов сталелитейного ...

СПОСОБ И СИСТЕМА ДЛЯ ПОЛУЧЕНИЯ БОТУЛИНИЧЕСКОГО НЕЙРОТОКСИНА

... 1. Способ хроматографии по существу APF для получения комплекса биологически активного ботулинического нейротоксина типа А, включающий следующие последовательные этапы:(а) культивацию бактерий Clostridium botulinum в питательной среде (по существу APF);(б) ферментацию бактерий Clostridium botulinum из питательной среды в примерно от 2 до примерно 75 л среды ферментации по существу APF, при этом, по меньшей мере, одна питательная среда и среда ферментации включают белок растительного происхождения;(в) сбор среды ферментации путем удаления остатков клеток, присутствующих в среде ферментации;(г) концентрацию собранной среды ферментации путем фильтрации;(д) разведение концентрированной среды ферментации путем добавления буфера;(е) первый этап контакта, в ходе которого разведенная собранная среда ферментации контактирует с анионообменной средой, таким образом, биологически активный ботулинический нейротоксин улавливается анионообменной средой;(ж) элюирование уловленного ботулинического нейротоксина ...

ОНКОЛИТИЧЕСКИЕ ШТАММЫ CLOSTRIDIUM GHONII, СПОСОБЫ ПОЛУЧЕНИЯ И ПРИМЕНЕНИЯ

... 1. Бактериальный штамм, полученный из авирулентного, непатогенного штамма Clostridium ghonii, где производный бактериальный штамм способен задерживать рост солидной опухоли или вызывать регрессию или разрушение солидной опухоли.2. Штамм по п. 1, где указанный штамм вызывает онколизис клеток, содержащихся в микроокружении опухоли.3. Штамм по любому из пп. 1-2, где штамм вызывает лизис от приблизительно 20% до приблизительно 90% клеток в микроокружении опухоли.4. Штамм по любому из пп. 1-2, где штамм вызывает лизис приблизительно 20% до приблизительно 70% клеток в микроокружении опухоли.5. Штамм по любому из пп. 1-2, где штамм вызывает лизис от приблизительно 20% до приблизительно 4 0% клеток в микроокружении опухоли.6. Штамм по любому из пп. 1-2, где штамм вызывает лизис от приблизительно 20% до приблизительно 35% клеток в микроокружении опухоли.7. Штамм по любому из пп. 1-2, где штамм вызывает лизис от приблизительно 25% до приблизительно 35% клеток в микроокружении опухоли.8. Штамм по ...

УСОВЕРШЕНСТВОВАННОЕ УЛАВЛИВАНИЕ УГЛЕРОДА ПРИ ФЕРМЕНТАЦИИ

... 1. Способ улавливания углерода при помощи микробиологической ферментации, включающий стадии, на которых ! i. принимают из производственного процесса поток (потоки) дымового или отходящего газа, содержащего СО; ! ii. направляют этот поток (потоки) газа в биореактор, в котором имеется культура одного или более микроорганизмов; ! iii. ферментируют эту культуру в биореакторе с получением одного или более продуктов. ! 2. Способ по п.1, включающий улавливание, по меньшей мере, части CO2 при помощи устройства для удаления СО2 из одного или обоих потоков: ! i. потока перед его вхождением в биореактор; ! ii. потока после его выхода из биореактора. ! 3. Способ по п.1, включающий первую стадию отделения газа, на которой: ! i. принимают поток газа; ! ii. по существу, отделяют, по меньшей мере, одну порцию этого потока газа, причем данная порция содержит один или более компонент этого потока газа; ! iii. направляют, по меньшей мере, часть отделенной порции(порций) в биореактор. ! 4. Способ по п.3, в ...

Композиции и способы, имеющие отношение к мутантному токсину из Clostridium Difficile

... 1. Культуральная среда, содержащая гидролизат сои, дрожжевой экстракт и глюкозу, где среда содержит бактерию Clostridium difficile.2. Среда по п. 1, дополнительно содержащая полиэтиленгликоль 2000.3. Среда по п. 1, дополнительно содержащая тиамфеникол.4. Среда по п. 1, где бактерия происходит из С. difficile VPI 11186.5. Среда по п. 1, где бактерия содержит полинуклеотид, кодирующий мутантный токсин из С. difficile, при этом бактерия дополнительно содержит промотор фередоксина (fdx) Clostridium sporogenes.6. Среда по п. 1, где бактерия содержит нуклеиновокислотную последовательность, кодирующую выделенный полипептид, который содержит аминокислотную последовательность, приведенную в любой из последовательностей SEQ ID NO: 1-761.7. Способ культивирования Clostridium difficile, включающий культивирование С. difficile в среде, содержащей гидролизат сои, дрожжевой экстракт и глюкозу, где среда содержит бактерию Clostridium difficile, происходящую из VPI 11186, где у данной бактерии отсутствует ...

Enhanced ABE fermentation with high yielding butanol tolerant Clostridium strains

Compositions comprising bacterial strains

Compositions comprising bacterial strains

Process

Compositions comprising bacterial strains

PROCEDURE FOR the PRODUCTION OF PRODUCTS WITH BACTERIAL ACTIVITY, WHICH IS TO TRANSFORMATION OF GLYCERIN IN 1,3-PROPANDIOL ABLE, AS WELL AS the APPROPRIATE TRUNKS AND THEIR APPLICATION FOR INDUSTRIALISTS the PRODUCTION OF 1,3-PROPANDIOL

Microorganisms for the production of 1,4-butanediol and related methods

The invention provides non-naturally occurring microbial organisms comprising a 1,4-butanediol (BDO) pathway comprising at least one exogenous nucleic acid encoding a BDO pathway enzyme expressed in a sufficient amount to produce BDO and further optimized for expression of BDO. The invention additionally provides methods of using such microbial organisms to produce BDO.

Continuous single vessel butanol synthesis by fermentation

The present invention describes a method for producing butanol by fermentation of carbohydrates using mixed populations of acidogenic-phase cells and solventogenic-phase cells of in a solitary vessel. The present system as described does not require intermittent adjustment of pH or venting of headspace gases. The method provides a process for removal of the butanol product which does not irreversibly harm the cells and conditions are described where such cells may resume butanol synthesis in the same solitary vessel. The invention also describes compositions and biologically pure cultures which comprise the cells as disclosed.

Microbial consortium and uses thereof

Provided herein are, ...

MICROORGANISM AND METHOD FOR IMPROVED 1,3-PROPANEDIOL PRODUCTION BY FERMENTATION ON A CULTURE MEDIUM WITH HIGH GLYCERINE CONTENT

The present invention concerns a new method for the production of 1,3- propanediol comprising culturing a recombinant microorganism converting glycerol into 1,3-propanediol and overexpressing the hcpR and/or frdX gene on a medium comprising glycerine. A recombinant microorganism for the production of 1,3 propanediol from glycerol, wherein said microorganism converts glycerol into 1,3-propanediol and 10 overexpresses the hcpR and/or the frdX gene.

PREPARATION OF A NOVEL NADP LINKED ALCOHOL- ALDEHYDE/KETONE OXIDOREDUCTASE FROM THERMOPHILIC ANAEROBIC BACTERIA FOR ANALYTICAL AND COMMERCIAL USE

PREPARATION OF A NOVEL NADP LINKED ALCOHOL-ALDEHYDE/ KETONE OXIDOREDUCTASE FROM THERMOPHILIC ANAEROBIC BACTERIA FOR ANALYTICAL AND COMMERCIAL USE A partically purified NADP specific thermostable alcohol, aldehyde/ketone oxidoreductase is prepared which can react with a wide range of alcohols, ketones and aldehydes. The enzyme has a unique preference for secondary over primary alcohols. The thermostability, broad range of operating temperatures and lack of sensitivity to metal ions and complexing agents, in addition to the absolute specifity for the coenzyme, increase the utility of the enzyme in asymmetric organic synthesis and NADPH regeneration.

RECOMBINANT MICROORGANISMS AND USES THEREFOR

Bacteria are genetically engineered to produce 3-hyrdoxypropionate (3-HP). The bacteria are carboxydotrophic acetogens. The bacteria produce acetyl-coA using the Wood-Ljungdahl pathway for fixing CO/C0?2#191. A malonyl-coA reductase from a bacterium that contains such an enzyme is introduced. Additionally, an acetyl-coA carboxylase may also be introduced The production of 3-HP can be improved by overproduction of acetyl-CoA carboxylase or by overproduction of biotin. This can be effected by improved promoters or higher copy number or enzymes that are catalytically more efficient.

RECOMBINANT MICROORGANISMS MAKE BIODIESEL

A carboxydotrophic acetogenic recombinant microorganism is modified so that it produces biodiesel and optionally one or more other products by fermentation of a substrate comprising CO. Biodiesel is produced by microbial fermentation of a substrate comprising CO. The recombinant microorganism is modified to express one or more exogenous enzymes in the biodiesel biosynthesis pathway not present in a parental microorganism from which the recombinant microorganism is derived. The one or more enzymes comprise a nonspecific acyltransferase.

EXTRACTION OF NITROGEN FROM ORGANIC MATERIALS THROUGH AMMONIFICATION BY MIXED BACTERIAL POPULATIONS

The invention provides a process for producing ammonia or ammonium from an organic material by fermenting a medium comprising organic material in the presence of a mixed bacterial population capable of ammonification, wherein the fermenting is under conditions, and for a sufficient period of time, to produce a fermentation product that comprises ammonia or ammonium. The organic material includes nitrogenous compounds suitable for conversion to ammonia or ammonium.

A PROCESS FOR CONTROLLING FERMENTATION OF CO-CONTAINING SUBSTRATES

A process for stable fermentation of CO-containing substrates and improved ethanol productivity includes providing medium components in amounts needed by microorganisms in the fermentation. The process includes determining a potassium concentration in the fermentation medium and providing a first medium and a second medium to the fermentation, the first medium provided at a rate effective for maintaining the potassium in the fermentation medium in a range of about 20 to about 200 mg/L until reaching a target cell density.

METHODS AND COMPOSITIONS FOR PRODUCING SOLVENTS

Described herein are methods, compositions and synthetic biology approach es for solvent production, including but not limited to butanol production. Described herein are recombinant bacteria and yeast strains which may be use d in production of a solvent, including but not limited to butanol, from lig nocellulosic and other plant-based feedstocks. Described herein are methods of producing solvents, including but not limited to butanol, using bacteria and yeast strains. Described herein are methods of producing organisms that display highly efficient butanol production.

A PROCESS FOR FERMENTING CO-CONTAINING GASEOUS SUBSTRATES IN A LOW PHOSPHATE MEDIUM EFFECTIVE FOR REDUCING WATER USAGE

A process is provided for fermenting CO-containing gaseous substrates in a low phosphate medium. The process includes blending a liquid medium that includes at least one transition metal element with a liquid medium that includes at least at least one other transition metal element and one non-metal element to provide a fermentation medium. The process is effective for preventing precipitation of one or more transition metal elements with one or more non-metal elements. The fermentation medium used in the process is prepared in a way that requires significantly lower amounts of water and reduced levels of phosphate.

METHODS TO CULTURE HUMAN GASTROINTESTINAL MICROORGANISMS

A media supplement for culturing anaerobic bacteria is provided which comprises a filtrate of effluent from a chemostat vessel in which a target bacterial ecosystem has been cultured. Methods of using the supplement for culturing or isolating anaerobic microbial strains or communities, particularly anaerobic bacteria from the human gut, are also provided.

HUMAN-DERIVED BACTERIA THAT INDUCE PROLIFERATION OR ACCUMULATION OF REGULATORY T CELLS

Species of human-derived bacteria belonging to the Clostridia class have been shown to induce accumulation of regulatory T cells (Treg cells) in the colon and suppress immune functions. Pharmaceutical compositions containing these bacteria can be used to prevent and treat immune-mediated diseases such as autoimmune diseases.

A NOVEL ETHANOLOGENIC CLOSTRIDIUM SPECIES, CLOSTRIDIUM COSKATII

A novel Clostridia bacterial species (Clostridium coskatii ATCC No. PTA- 10522, "PS02") is provided. Under anaerobic conditions C. coskatii can convert CO and/or H2 and/or CO2 to ethanol or acetate. Thus, this novel bacterium is capable of transforming waste gases (e.g. syngas and refinery wastes) into useful products.

Methods and compositions for affecting the differentiation of clostridia in culture

The invention relates generally to methods and compositions for maintaining and manipulating microbial cultures of Gram-positive bacteria. Also provided are methods for identifying quorum sensing regulatory proteins and auto-inducing peptides in Gram-positive bacteria. Also provided are methods and compositions for affecting quorum sensing pathways of the genus Clostridium in culture including auto-inducing peptides to direct or maintain Clostridium cultures in a desired differentiated state. Differentiated states include extended serial propagation for the production of butanol or other fermentation products.

Microorganisms for 1,3-propanediol production using high glycerine concentration

The present invention is related to a population of Clostridium acetobutylicum useful for the production of 1,3-propanediol (PDO), wherein said population comprises at least one strain of a Clostridium acetobutylicum sp. comprising mutations selected among the mutations identified in table 1, wherein relative percentages of said mutations are selected among specific genes.

Carbon capture in fermentation

The invention relates to methods of capturing carbon by microbial fermentation of a gaseous substrate comprising CO. The methods of the invention include converting CO to one or more products including alcohols and/or acids and optionally capturing CO2 to improve overall carbon capture. In certain aspects, the invention relates to processes for producing alcohols, particularly ethanol, from industrial waste streams, particularly steel mill off-gas.

Recombinant microorganisms and uses therefor

Bacteria are genetically engineered to produce 3-hydroxypropionate (3-HP). The bacteria are carboxydotrophic acetogens. The bacteria produce acetyl-coA using the Wood-Ljungdahl pathway for fixing CO/CO 2 . A malonyl-coA reductase from a bacterium that contains such an enzyme is introduced. Additionally, an acetyl-coA carboxylase may also be introduced The production of 3-HP can be improved by overproduction of acetyl-CoA carboxylase or by overproduction of biotin. This can be effected by improved promoters or higher copy number or enzymes that are catalytically more efficient.

Recombinant microorganisms make biodiesel

A carboxydotrophic acetogenic recombinant microorganism is modified so that it produces biodiesel and optionally one or more other products by fermentation of a substrate comprising CO. Biodiesel is produced by microbial fermentation of a substrate comprising CO. The recombinant microorganism is modified to express one or more exogenous enzymes in the biodiesel biosynthesis pathway not present in a parental microorganism from which the recombinant microorganism is derived. The one or more enzymes comprise a nonspecific acyltransferase.

Carbon capture in fermentation

The invention relates to a steel mill adapted to provide gas stream(s) comprising CO to a microbial fermentation, the steel mill comprising a steel mill structure containing apparatus for a steel manufacturing process wherein said apparatus produces waste gases during various stages of the steel making process, said waste gases being directed into the atmosphere by a waste stack, wherein the waste stack is connected to a fermentation system by a transfer means connected to the waste stack to divert at least a portion of the waste gases to the microbial fermentation system.

COMPOSITIONS COMPRISING BACTERIAL STRAINS

The invention provides compositions comprising a bacterial strain of the species or , for use in therapy. 115.-. (canceled)16Blautia productaBlautia coccoides. A method of treating one or more symptoms of a gastrointestinal inflammatory disease in a subject in need thereof , comprising administering to the subject a therapeutically effective amount of a composition comprising a bacterial strain of the species or , wherein the bacterial strain comprises a 16s rRNA gene sequence having at least 95% sequence identity to SEQ ID NO: 1 , wherein the one or more symptoms of the gastrointestinal inflammatory disease comprises ulcerations and/or bleeding , weight loss , poor weight gain , or increased gut permeability , and wherein the administering is effective to treat the one or more symptoms of the gastrointestinal inflammatory disease in the subject.17. The method of claim 16 , wherein the gastrointestinal inflammatory disease comprises colitis claim 16 , Crohn's disease claim 16 , or inflammatory bowel disease (IBD).18. The method of claim 17 , wherein the colitis comprises ulcerative colitis.19. The method of claim 18 , wherein the ulcerative colitis comprises distal colitis claim 18 , proctitis claim 18 , proctosigmoiditis claim 18 , left-sided colitis claim 18 , extensive colitis claim 18 , or pancolitis.20. The method of claim 17 , wherein the Crohn's disease comprises Ileocolic Crohn's claim 17 , Crohn's ileitis claim 17 , or Crohn's colitis.21. The method of claim 16 , wherein the administering is effective to reduce ulcerations and/or bleeding claim 16 , reduce weight loss claim 16 , enhance weight gain claim 16 , or reduce gut permeability in the subject with the gastrointestinal inflammatory disease claim 16 , thereby treating the gastrointestinal inflammatory disease.22. The method of claim 16 , wherein the therapeutically effective amount comprises from about 1×10to about 1×10colony forming units (CFU).23. The method of claim 16 , wherein the composition ...

PROCESS AND SYSTEM FOR OBTAINING BOTULINUM NEUROTOXIN

Rapid, animal protein free, chromatographic processes and systems for obtaining high potency, high yield botulinum neurotoxin for research, therapeutic and cosmetic use. 1. A substantially animal product free (APF) process utilizing chromatography for obtaining a biologically active botulinum neurotoxin , the process comprising the following steps:(a) providing a substantially APF fermentation medium;{'i': 'Clostridium botulinum', '(b) fermenting bacteria in the fermentation medium, and;'}(c) recovering the biologically active botulinum neurotoxin from the fermentation medium by contacting the fermentation medium with an anion exchange chromatography media followed by contacting an eluent from the anion exchange chromatography medium with a cation exchange chromatography, thereby obtaining the biologically active botulinum neurotoxin from the substantially APF chromatography process.2. The process of claim 1 , wherein the botulinum neurotoxin obtained comprises one part or less residual nucleic acid per million of the botulinum neurotoxin obtained.3. The process of claim 1 , wherein the process is carried out in one week or less.4. A biologically active botulinum neurotoxin obtained by the process of .5. A substantially APF chromatographic process for obtaining a biologically active botulinum neurotoxin type A complex claim 1 , the process comprising the following sequential steps:{'i': 'Clostridium botulinum', '(a) culturing bacteria in a substantially APF culture medium;'}{'i': 'Clostridium botulinum', '(b) fermenting bacteria from the culture medium in about 2 L to about 75 L of a substantially APF fermentation medium, wherein at least one of the culture medium and the fermentation medium includes a vegetable protein;'}(c) harvesting the fermentation medium by removing cellular debris present in the fermentation medium;(d) concentrating the harvested fermentation medium by filtration;(e) diluting the concentrated fermentation medium by adding a buffer;(f) a first ...

Devices, systems and methods for the production of humanized gut commensal microbiota

One embodiment provides a commensal gut production platform for ex vivo production of human gut commensal microbiota. Another embodiment provides devices, systems and methods for ex vivo culturing of gut microflora in a system that mimics the human gut environment. The culturing of the commensal microbiota in the disclosed systems produces gut microbiota having defined characteristics and properties that can be exploited to treat various conditions in a subject.

Human-derived bacteria that induce proliferation or accumulation of

Species of human-derived bacteria belonging to the Clostridia class have been shown to induce accumulation of regulatory T cells (Treg cells) in the colon and suppress immune functions. Pharmaceutical compositions containing these bacteria can be used to prevent and treat immune-mediated diseases such as autoimmune diseases.

Methods and compositions relating to microbial treatment and diagnosis of disorders

The present disclosure provides methods, systems, compositions, and kits to address the need for microbiome-related treatment of health conditions and disease. The present disclosure provides for treatment of metabolic conditions using microbial compositions.

Feed ingredient comprising clostridium tyrobutyricum

Provided is a feed ingredient comprising at least 20% by weight Clostridium tyrobutyricum, wherein said Clostridium tyrobutyricum comprises at least 70% of dry weight crude protein and optionally at least 7% of dry weight lysine and/or at least 1% of dry weight methionine. Further provided are animal feeds and probiotic compositions comprising the feed ingredient, methods for the production thereof and uses thereof.

SOURDOUGH PRODUCT

The present invention provides products and methods for improving the tolerance of a dough in bakery and patisserie products. More specifically it provides sourdough products obtained through the fermentation of cereals by specific strains of bacteria. The products obtained by using said sourdough products are characterized by having a strengthened dough resulting in bakery and patisserie products with improved physical properties and taste. 1Clostridium saccharobutylicum.. A sourdough product comprising cereal or cereal fractions , wherein said sourdough product is fermented by one or more strains of2Clostridium saccharobutylicumClostridium saccharobutylicum. The sourdough product according to claim 1 , wherein said strain of is DSM 13864.3. The sourdough product according to claim 1 , wherein said sourdough product is fermented by one or more additional microorganisms chosen from lactic acid forming bacteria claim 1 , in particular slow acidifying lactic acid bacteria claim 1 , and/or yeast strains.4Lactobacillus sakei, Lactobacillus crustorumLactobacillus reuteriLactobacillus sakeiLactobacillus crustorum.. The sourdough product according to claim 3 , wherein said additional microorganism is or claim 3 , preferably or5. The sourdough product according to claim 1 , wherein said sourdough product is a liquid sourdough product claim 1 , a paste sourdough product or a dried sourdough product.6. The sourdough product according to claim 1 , wherein said sourdough product is an active or inactive sourdough product.7. The sourdough product according to claim 1 , wherein said sourdough product is a liquid sourdough product characterized by having a pH between 4.2 and 9.0 claim 1 , preferably between 4.2 and 6.0 claim 1 , more preferably between 4.2 and 4.8.8. The sourdough product according to claim 1 , wherein said sourdough product is a dried sourdough product obtained by drying a liquid sourdough product.9. The sourdough product according to claim 1 , further comprising ...

Method for producing organic substance

Provided is a method which allows, for example, suppression of foaming in the purification step such as distillation and continuous operation, as well as direct treatment of a waste liquid (can liquid) without having to subject the same to an extra purification treatment by removing the microorganisms, nitrogen compounds, and phosphorous compounds at once from an organic substance-containing liquid obtained from microbial fermentation. Also disclosed is a method for producing an organic substance, comprising a microbial fermentation step, a separation step, a liquefaction step, and a second purification step, wherein the concentration of the nitrogen compound in the second can liquid is 0 to 150 ppm based on the total mass of the second can liquid, and the concentration of the phosphorous compound in the second can liquid is 0 to 5 ppm based on the total mass of the second can liquid.

ETHANOL

The present disclosure provides ethanol comprising an inorganic component and/or an organic component. The inorganic component may contain at least one component selected from the group consisting of: silicon having a content of 10 mg/L or more and 100 mg/L or less; chromium having a content of 0.6 mg/L or less; iron having a content of 2.0 mg/L or less; sodium having a content of 150 mg/L or more and 1000 mg/L or less; and potassium having a content of 1.0 mg/L or more and 10 mg/L or less. The organic component may contain at least one component selected from the group consisting of: aliphatic hydrocarbon having a content of 0.16 mg/L or more and 10 mg/L or less; aromatic hydrocarbon having a content of 0.4 mg/L or more and 10 mg/L or less; and dialkyl ether having a content of 0.1 mg/L or more and 100 mg/L or less. 1. Ethanol comprising an inorganic component and/or an organic component , whereinthe inorganic component comprises at least one component selected from the group consisting of:silicon having a content of 10 mg/L or more and 100 mg/L or less;chromium having a content of 0.6 mg/L or less;iron having a content of 2.0 mg/L or less;sodium having a content of 150 mg/L or more and 1000 mg/L or less; andpotassium having a content of 1.0 mg/L or more and 10 mg/L or less; andthe organic component comprises at least one component selected from the group consisting of:aliphatic hydrocarbon having a content of 0.16 mg/L or more and 10 mg/L or less;aromatic hydrocarbon having a content of 0.4 mg/L or more and 10 mg/L or less; anddialkyl ether having a content of 0.1 mg/L or more and 100 mg/L or less.2. The ethanol according to claim 1 , whereina substrate is a gas comprising carbon monoxide and hydrogen.3. The ethanol according to claim 1 , derived from microbial fermentation.4. The ethanol according to claim 2 , whereinthe gas comprising carbon monoxide and hydrogen is derived from waste.5. A method for manufacturing ethanol claim 2 , comprising:a step of ...

An apparatus for producing an organic substance

Provided is a device for continuously manufacturing an organic substance with high productivity, in which a means is installed whereby a synthesis gas having a specific nutrient source concentration for microbes in a continuous fermenter is stored with good efficiency and the gas composition thereof is made uniform in a stage prior to supplying the synthesis gas to the fermenter including microbes, so that uniformity of the synthesis gas composition is ensured. A device for manufacturing an organic substance, provided with a gas generating unit for gasifying a carbon source and generating a starting material gas, a gas supply unit for supplying a synthesis gas including the starting material gas to a fermenter, and a fermenter for microbial fermentation of the synthesis gas in the fermenter, a gas circulation unit for making the gas composition uniform being further provided between the gas generating unit and the gas supply unit or to the gas supply unit.

FUNCTIONAL FEED

A feed including cashew nut shell liquid and bacterial cells is provided for raising non-human monogastric animals such as chickens or pigs, wherein the cashew nut shell liquid content is not more than 500 ppm by mass with respect to the total amount of the feed. The feed can stimulate weight gain in non-human monogastric animals such as chickens or pigs and increase the efficiency in, for example, meat production.

PROCESS AND SYSTEM FOR OBTAINING BOTULINUM NEUROTOXIN

Rapid, animal protein free, chromatographic processes and systems for obtaining high potency, high yield botulinum neurotoxin for research, therapeutic and cosmetic use. 112-. (canceled)13Clostridial botulinum. A substantially animal product free (APF) process for purifying and obtaining approximately 150 kDa serotype A (BoNT/A) in a clarified culture comprising BoNT/A complex and at least one impurity protein , said process comprising:(a) directly loading an anion exchange chromatography column with the clarified culture to afford a first purified sample;(b) contacting the first purified sample with a cation exchange chromatography column to afford a second purified sample;(c) contacting the second purified sample with an aqueous solution having a pH between about 7.0 and about 8.0 to provide a third purified sample comprising dissociated approximately 150 kDa BoNT/A;(d) contacting the third purified sample with one or more additional chromatography columns selected from an ion exchange chromatography column, a gel filtration chromatography column, or an affinity column; and(e) recovering the dissociated approximately 150 kDa BoNT/A.14. The method of claim 13 , wherein the clarified culture has been prepared without an acid precipitation step.15. The method of claim 13 , wherein the one or more additional chromatography columns comprise a third chromatography column and a fourth chromatography column.16. The method of claim 15 , wherein the third chromatography column is a subsequent cation exchange chromatography column.17. The method of claim 15 , wherein the fourth chromatography column is a gel filtration chromatography column.18. The method of claim 13 , wherein step (a) further comprises contacting the clarified culture with a first buffer on the anion exchange chromatography column.19. The method of claim 18 , wherein the first buffer is phosphate buffer.20. The method of claim 18 , wherein the first buffer has a pH of about 6.5.21. The method of claim 13 , ...

CLOSTRIDIA CONSORTIA COMPOSITIONS AND METHODS OF TREATING OBESITY, METABOLIC SYNDROME AND IRRITABLE BOWEL DISEASE

Disclosed herein, are compositions comprising a consortium of Clostridia, and methods of treating obesity, metabolic syndrome, irritable bowel disease, as well as reducing weight gain and inhibiting lipid absorption in the small intestine by administering the compositions to a subject. 1consortium.. A composition comprising a supernatant from a Clostridia2consortiumanaerovorax, ClostridiumClostridiumLachnospiraceae. The composition of claim 1 , wherein the Clostridia comprises two or more strains of bacterium claim 1 , wherein the two or more strains of bacterium are Clostridia XIVa claim 1 , IV and spps.3consortiumanaerovorax, ClostridiumClostridiumLachnospiraceae. The composition of claim 1 , wherein the composition is capable of suppressing expression of lipid absorption genes within intestinal epithelia in a subject claim 1 , and wherein the Clostridia comprises two or more of Clostridia XIVa claim 1 , IV and spps.4consortiumanaerovorax, ClostridiumClostridiumLachnospiraceae. The composition of claim 1 , wherein the composition is capable of inhibiting lipid absorption in a subject's small intestine claim 1 , and wherein the Clostridia comprises two or more of Clostridia XIVa claim 1 , IV and spps.5consortiumanaerovorax, ClostridiumClostridiumLachnospiraceae. The composition of claim 1 , wherein the composition is capable of reducing weight gain in a subject claim 1 , and wherein the Clostridia comprises two or more of Clostridia XIVa claim 1 , IV and spps.6consortiumanaerovorax, ClostridiumClostridiumLachnospiraceae. The composition of claim 1 , wherein the composition is capable of downregulating CD36 in a subject's liver claim 1 , and wherein the Clostridia comprises two or more of Clostridia XIVa claim 1 , IV and spps.7consortiumanaerovorax, ClostridiumClostridiumLachnospiraceae. The composition of claim 1 , wherein the composition is capable of reducing adiposity in a subject claim 1 , and wherein the Clostridia comprises two or more of Clostridia XIVa ...

EXTREME THERMOPHILIC BACTERIA OF THE GENUS CALDICELLULOSIRUPTOR SUITABLE FOR THE CONVERSION OF CELLULOSIC AND STARCHY BIOMASS

Isolated cellulolytic extreme thermophilic bacterial cells belonging to the genus , mutants thereof, isolated strains, microbial cultures and microbial compositions. The novel bacteria are in particular suitable for the production of fermentation products such as lactic acid from any carbon source, not limited to cellulosic material but especially useful for converting cellulosic biomass like lignocellulosic biomass and/or starch containing biomass. 129-. (canceled)30CaldicellulosiruptorCaldicellulosiruptor, Caldicellulosiruptor, CaldicellulosiruptorCaldicellulosiruptor, Caldicellulosiruptor, CaldicellulosiruptorCaldicellulosiruptor. A method for converting lignocellulosic biomass and/or starch containing biomass to a carboxylic acid comprising the step of contacting the lignocellulosic biomass and/or the starch containing biomass with a microbial culture for a period of time at an initial temperature and an initial pH , thereby producing an amount of a carboxylic acid; wherein the microbial culture comprises an extremely thermophilic bacteria strain of the genus , wherein the strain is selected from the group consisting of sp. BluConL70 having the DSMZ Accession number 33496sp. BluConL60 having the DSMZ Accession number 33252sp. BluCon085 having the DSMZ Accession number 33485 sp. BluCon052 having the DSMZ Accession number 33470sp. BluCon006 having the DSMZ Accession number 33095sp. BluCon014 (DSMZ Accession number 33096) and sp. BluCon016 (DSMZ Accession number 33097) , microorganism derived therefrom , progenies or mutants thereof , wherein the mutants thereof retaining the properties of BluCon0L70 , BluConL60 , BluCon085 , BluCon052 , BluCon006 , BluCon014 and/or BluCon016.3136-. (canceled)37Miscanthus. The method according to claim 30 , wherein the lignocellulosic biomass is selected from the group consisting of grass claim 30 , switch grass claim 30 , cord grass claim 30 , rye grass claim 30 , reed canary grass claim 30 , mixed prairie grass claim 30 , claim ...

PROCESS AND SYSTEM FOR OBTAINING BOTULINUM NEUROTOXIN

Rapid, animal protein free, chromatographic processes and systems for obtaining high potency, high yield neurotoxin for research, therapeutic and cosmetic use. 112-. (canceled)14. The composition of claim 13 , wherein the drug substance is essentially APF.15. The composition of claim 13 , wherein the drug substance is entirely APF.16Clostridium botulinum.. The composition of claim 13 , wherein the substantially APF culture claim 13 , fermentation claim 13 , and purification process utilizes a Hall A strain of17. The composition of claim 13 , wherein the plurality of chromatography columns comprises one or more chromatography columns selected from the group consisting of ion-exchange chromatography (IEX) columns claim 13 , gel filtration chromatography columns claim 13 , affinity chromatography columns claim 13 , and hydrophobic interaction chromatography (HIC) columns.18. The composition of claim 13 , wherein the plurality of chromatography columns comprises an anion exchange chromatography (AEX) column followed by a cation exchange chromatography (CEX) column.19. The composition of claim 17 , wherein the plurality of chromatography columns comprises a hydrophobic interaction chromatography (HIC) column.20. The composition of claim 13 , wherein the composition is free of benzamidine.21. The composition of claim 13 , wherein the composition comprises about 0.5 mg of HSA.22. The composition of claim 13 , wherein the composition comprises about 0.9 mg of sodium chloride.23. The composition of claim 13 , wherein the composition is lyophilized or vacuum dried.25. The composition of claim 24 , wherein the drug substance is essentially APF.26. The composition of claim 24 , wherein the drug substance is entirely APF.27. The composition of claim 24 , wherein the plurality of chromatography columns comprises one or more chromatography columns selected from the group consisting of ion-exchange chromatography columns claim 24 , gel filtration chromatography columns claim 24 , ...

Novel cell penetrating peptides and uses thereof

Disclosed herein is a cosmetic composition including novel cell penetrating peptides, cell penetrating botulinum toxin recombinant proteins in which the cell-penetrating peptide and botulinum toxin are fused, polynucleotides encoding the cell penetrating botulinum toxin recombinant proteins, recombinant expression vector comprising the polynucleotides, and the cell penetrating botulinum toxin recombinant protein as active ingredients.

Process and system for obtaining botulinum neurotoxin

Rapid, animal protein free, chromatographic processes and systems for obtaining high potency, high yield botulinum neurotoxin for research, therapeutic and cosmetic use.

Method for producing functional substance

An object of the present invention is to a method for producing a functional substance utilizing a reaction requiring hydrogen, which production method is safe and efficient; and this object is achieved by a method for producing a functional substance utilizing a reaction requiring hydrogen, the method comprising: supplying the hydrogen by culturing of a hydrogen-producing microorganism.

Method of producing higher alcohols

The present invention relates to a reaction mixture and a method of producing at least one higher alcohol comprising a reaction mixture comprising a mixed culture of a first and a second microorganism in an aqueous medium comprising carbon monoxide gas, wherein the first microorganism is an acetogenic microorganism capable of converting a carbon source to acetate and/or ethanol; and the second microorganism is selected from the group consisting of Clostridium kluyveri , and C. Carboxidivorans capable of converting the acetate and/or ethanol to form an acid; wherein the first microorganism is further capable of converting the acid to the corresponding higher alcohol and the higher alcohol comprises at least 6 carbon atoms.

Aerobic method of producing alcohols

A reaction mixture for producing ethanol and/or acetate from a carbon source in aerobic conditions, wherein the mixture comprises a first acetogenic microorganism in an exponential growth phase; free oxygen; and a second acetogenic microorganism in a stationary phase wherein the first and second acetogenic microorganism is capable of converting the carbon source to the acetate and/or ethanol.

Novel clostridium sp. strain producing hexanoic acid in high yield and method for producing hexanoic acid using the same

The present disclosure relates to a Clostridium sp. JS66 strain producing metabolites having 4 to 6 carbon atoms in a high yield. The strain produces metabolites having 6 carbon atoms in a significantly high yield while reducing the production of acetic acid and ethanol as by-products.

Obligately anaerobic acetic acid-producing microorganism, and recombinant microorganism

Provided is a modified obligately anaerobic acetogen in which activity of at least one enzyme selected from the group consisting of a restriction enzyme, a modification enzyme, and a recognition enzyme constituting a type I restriction modification system enzyme is deleted or suppressed. Preferably, the activity of at least the restriction enzyme is deleted or suppressed. The obligately anaerobic acetogen is preferably a Clostridium bacterium or a Moorella bacterium, and particularly preferably Clostridium ljungdahlii.

Microbiological treatment system for ethylene oxide exhaust gas

The present disclosure provides a microbiological treatment system including a hydration system and microbiological degradation systems. The hydration system may include a gas-liquid mixing chamber, a gas inlet, a gas outlet, a liquid inlet, and a liquid outlet, the latter four being fluidly coupled to the chamber. The gas inlet is configured to introduce an ethylene oxide exhaust gas into the chamber to mix with an aqueous solution to form an ethylene oxide exhaust liquor. The liquid outlet is configured to discharge the ethylene oxide exhaust liquor. Each microbiological degradation system may include a degradation chamber containing degradation bacteria including one of anaerobic bacteria, facultative bacteria, or aerobic bacteria. The degradation chambers of the microbiological degradation systems may be in fluid communication sequentially in a predetermined degradation sequence, with the most upstream in the degradation sequence having a liquid inlet in fluid communication with the liquid outlet.

Compositions comprising bacterial strains

The invention provides compositions comprising bacterial strains for treating and preventing inflammatory and autoimmune diseases.

Recombinant microorganisms and methods of use thereof

Provided is a method of producing a product by culturing a carboxydotrophic acetogenic bacterium with a disrupting mutation in a lactate dehydrogenase enzyme in the presence of a substrate comprising CO, CO 2 , and/or H 2 . Preferably, the disrupting mutation reduces or eliminates the expression or activity of the enzyme such that the bacterium produces a reduced amount of lactate or no lactate.

Use of galacturonate and or galacturonate polymers in conjunction with carbohydrates to control metabolic state of organisms

A method of producing chemicals includes providing fermentative cells; co-feeding any of galacturonate and galacturonate polymers with carbohydrates to the fermentative cells; and producing a chemical from the fermentative cells. The fermentative cells may include any of Clostridium acetobutylicum and Clostridium saccharoperbutylacetonicum. The carbohydrates may include any of glucose, mannose, galactose, fructose, arabinose, xylose, sucrose, lactose, maltose, cellobiose, and starch. The method may include providing a substantially equal proportion of the any of galacturonate and galacturonate polymers and the carbohydrates for co-feeding to the fermentative cells. The method may include altering a proportion of the any of galacturonate and galacturonate polymers to the carbohydrates. The method may include modulating a production of the chemical by altering the proportion of the any of galacturonate and galacturonate polymers to the carbohydrates. The chemical may include any of acetate and butyrate.

Method for treatment of disorders of the gastrointestinal system

There are provided novel synthetic stool preparations comprising bacteria isolated from a fecal sample from a healthy donor. The synthetic stool preparations are used for treating disorders of the gastrointestinal tract, including dysbiosis, Clostridium difficile infection and recurrent Clostridium difficile infection, prevention of recurrence of Clostridium difficile infection, treatment of Crohn's disease, ulcerative colitis, irritable bowel syndrome, inflammatory bowel disease, and diverticular disease, and treatment of food poisoning such as salmonella . Methods of preparation and methods of use of the synthetic stool preparations are also provided.

Specific bacteria for their use as a medicament, in particular for controlling excess weight, obesity, cardiometabolic diseases and inflammatory bowel diseases

The object of the invention are compositions and medical treatment methods with an inheritable, Gram-negative, strictly anaerobic and commensal bacterium of the family Christensenellaceae belonging to an OTU (Operational Taxonomic Unit) characterized by a 16S rRNA sequence SEQ ID NO: 1 or to an OTU characterized by a 16S rRNA sequence SEQ ID NO: 2.

COMPOSITIONS COMPRISING BACTERIAL STRAINS

The invention provides compositions comprising one or more bacterial strains for treating or preventing sensory hypersensitivity. 115.-. (canceled)16Blautia hydrogenotrophica. A method of treating fibromyalgia in a subject in need thereof , comprising administering to the subject a composition comprising a therapeutically effective amount of a bacterial strain of the species , and wherein the administering is effective to treat the fibromyalgia.17. The method of claim 16 , wherein the subject has not been diagnosed with visceral hypersensitivity.18. The method of claim 16 , wherein the therapeutically effective amount comprises from about 1×10to about 1×10colony forming units (CFU).19. The method of claim 16 , wherein the administering is oral.20. The method of claim 16 , wherein the composition further comprises a pharmaceutically acceptable excipient or carrier.21. The method of claim 16 , wherein the bacterial strain is lyophilized.22. The method of claim 16 , wherein the bacterial strain is viable.23. The method of claim 16 , wherein the composition comprises the bacterial strain as part of a microbial consortium.24. The method of claim 16 , wherein the composition comprises de minimis or biologically irrelevant amounts of other bacterial strains or species.25. The method of claim 16 , wherein the composition comprises a single bacterial species or a single bacterial strain.26Clostridium.. The method of claim 16 , wherein the composition does not comprise a bacterial strain of the genus27. The method of claim 16 , wherein the bacterial strain comprises a 16s rRNA gene sequence having at least 95% sequence identity to the polynucleotide sequence of SEQ ID NO: 5.28. The method of claim 16 , wherein the bacterial strain is the strain deposited under accession number DSM 14294 or a biotype thereof.29Blautia hydrogenotrophica. A method of treating sensory hypersensitivity in a subject diagnosed with fibromyalgia claim 16 , comprising administering to the subject a ...

Compositions and Methods for the Conversion of Short-Chained Carboxylic Acids to Alcohols Using Clostridial Enzymes

The invention relates to the fields of bacterial metabolism and the utilization or consumption of short-chain carboxylic acids to reduced products. Specifically, it relates to syngas fermentations using monocultures of syngas-utilizing homoacetogenic bacteria for the production of alcohols using native alcohol dehydrogenase.

Media Supplements and Methods to Culture Human Gastrointestinal Anaerobic Microorganisms

A media supplement for culturing anaerobic bacteria is provided which comprises a filtrate of eilluent from a chemostat vessel in which a target bacterial ecosystem has been culnn-ed. Methods of using the supplement for culturing or isolating anaerobic microbial strains or cormmmities, particularly anaerobic bacteria from the human gut, are also provided.

Methods and compositions relating to microbial treatment and diagnosis of disorders

The present disclosure provides methods, systems, compositions, and kits to address the need for microbiome-related treatment of health conditions and disease. The present disclosure provides for treatment of metabolic conditions using microbial compositions.

Integrated wet-mill method for the production of ethanol and single cell protein

An integrated wet-mill method for the production of ethanol and single cell protein. An integrated method for the production of ethanol and a single cell protein product is described including providing corn kernels, which kernels comprise germ, fiber, protein and starch and which method includes, among other things, steeping said corn kernels and separating said steeped kernels from a steep liquor.

Microorganism and method for improved 1,3-propanediol production by fermentation on a culture medium with high glycerine content

The present invention concerns a new method for the production of 1,3-propanediol comprising culturing a recombinant microorganism converting glycerol into 1,3-propanediol and overexpressing the hcpR and/or frdX gene on a medium comprising glycerine. A recombinant microorganism for the production of 1,3 propanediol from glycerol, wherein said microorganism converts glycerol into 1,3-propanediol and 10 overexpresses the hcpR and/or the frdX gene.

Microorganisms for the production of 1,4-butanediol and related methods

The invention provides non-naturally occurring microbial organisms comprising a 1,4-butanediol (BDO) pathway comprising at least one exogenous nucleic acid encoding a BDO pathway enzyme expressed in a sufficient amount to produce BDO and further optimized for expression of BDO. The invention additionally provides methods of using such microbial organisms to produce BDO.

Isolation of microbial cell lines for organic acid production

Microbial cell lines suitable for industrial-scale production of organic acids and methods of making and isolating such cell lines.

Isolation and characterization of novel clostridial species

A novel clostridia bacterial species ( Clostridium ragsdalei, ATCC BAA-622, “P11”) is provided. P11 is capable of synthesizing, from waste gases, products which are useful as biofuel. In particular, P11 can convert CO to ethanol. Thus, this novel bacterium transforms waste gases (e.g. syngas and refinery wastes) into useful products. P11 also catalyzes the production of acetate.

Microbiological treatment system for ethylene oxide exhaust gas

A microbiological treatment system (1000) includes a hydration system (1) and microbiological degradation systems (2). The hydration system (1) may include a gas-liquid mixing chamber (106), a gas inlet (102),a gas outlet (103), a liquid inlet (104), and a liquid outlet (105), the latter four being fluidly coupled to the chamber (106). The gas inlet (102) is configured to introduce an EO exhaust gas into the chamber (106) to mix with an aqueous solution to form an EO exhaust liquor. The liquid outlet (105) is configured to discharge the EO exhaust liquor. Each microbiological degradation system (2) may include a degradation chamber (21, 22, 23) containing degradation bacteria including one of anaerobic bacteria, facultative bacteria, or aerobic bacteria. The degradation chambers (21, 22, 23) of the microbiological degradation systems (2) may be in fluid communication sequentially in a predetermined degradation sequence, with the most upstream in the degradation sequence having a liquid inlet in fluid communication with the liquid outlet (105).

Method for preparing botulinum toxin

본 발명은 동물유래 성분을 포함하지 않는 단순화된 공정으로 보툴리눔 독소를 고수율로 수득할 수 있는 보툴리눔 독소의 제조방법에 관한 것이다. 본 발명에 따른 보툴리눔 독소의 제조방법은 클로스트리디움 보툴리눔 균주의 배양을 비롯한 전체 공정에서 동물성 성분을 사용하지 않아 안전성이 우수하며, 종래 분리 공정과 비교하여 첨가제 처리를 통한 별도의 핵산제거 과정을 생략하고 또한 이온교환 크로마토그래피만을 이용하여 공정을 수행하며 이때 모두 동일한 버퍼를 사용하여 버퍼의 농도와 pH 조절만으로 단순화된 공정을 통해 현저히 향상된 수율로 보툴리눔 독소를 분리할 수 있음을 확인하였는바, 매우 경제적이고 효율적인 분리방법으로써 이를 통해 분리된 보툴리눔 독소는 미용 및 의약분야에서 유용하게 이용될 수 있을 것으로 기대된다. The present invention relates to a method for producing a botulinum toxin capable of obtaining a botulinum toxin in a high yield by a simplified process that does not include animal-derived components. The method for producing a botulinum toxin according to the present invention is excellent in safety because it does not use animal components in the entire process including the cultivation of Clostridium botulinum strain, and a separate nucleic acid removal process through additive treatment is omitted compared to the conventional separation process. In addition, the process is performed using only ion exchange chromatography, and it was confirmed that the botulinum toxin can be separated with a significantly improved yield through a simplified process only by adjusting the concentration and pH of the buffer using the same buffer, which is very economical. As an efficient and efficient separation method, the isolated botulinum toxin is expected to be usefully used in the cosmetic and pharmaceutical fields.

Aerobic method of producing alcohols

FIELD: chemistry.SUBSTANCE: group of inventions relates to a reaction mixture for producing ethanol and/or acetate from a carbon source in aerobic conditions and use thereof. Disclosed is a reaction mixture for producing ethanol and/or acetate from a carbon source in aerobic conditions, comprising a first acetogenic microorganism in an exponential growth phase, free oxygen in concentration from 0.000005 % to 1 % by volume and second acetogenic microorganism in stationary phase. First and second acetogenic microorganisms are capable of converting a carbon source into acetate and/or ethanol. First and second microorganisms are selected from a group consisting of. First and second acetogenic microorganisms are identical microorganisms. Disclosed also is use of a mixture for producing ethanol and/or acetate.EFFECT: group of inventions provides efficient production of ethanol and/or acetate from a carbon source under aerobic conditions.5 cl, 3 tbl, 12 ex РОССИЙСКАЯ ФЕДЕРАЦИЯ (19) RU (11) (13) 2 724 532 C2 (51) МПК C12N 1/20 (2006.01) C12P 7/04 (2006.01) C12P 7/06 (2006.01) C12P 7/14 (2006.01) C12P 7/40 (2006.01) ФЕДЕРАЛЬНАЯ СЛУЖБА ПО ИНТЕЛЛЕКТУАЛЬНОЙ СОБСТВЕННОСТИ (12) ОПИСАНИЕ ИЗОБРЕТЕНИЯ К ПАТЕНТУ (52) СПК C12N 1/20 (2020.02); C12P 7/04 (2020.02); C12P 7/06 (2020.02); C12P 7/14 (2020.02); C12P 7/40 (2020.02) (21)(22) Заявка: 2016102677, 27.01.2016 (24) Дата начала отсчета срока действия патента: Дата регистрации: 23.06.2020 (73) Патентообладатель(и): Эвоник Оперейшенс ГмбХ (DE) 28.01.2015 EP 15152866.8 (43) Дата публикации заявки: 28.07.2017 Бюл. № 22 (56) Список документов, цитированных в отчете о поиске: US 20120009638 А1, 12.01.2012. WO 2008028055 A2, 06.03.2008. US 2007275447 A1, 29.11.2007. RU 2394913 С2, 20.07.2010. (45) Опубликовано: 23.06.2020 Бюл. № 18 2 7 2 4 5 3 2 (54) АЭРОБНЫЙ СПОСОБ ПОЛУЧЕНИЯ СПИРТОВ (57) Реферат: Группа изобретений относится к реакционной woodii (DSM 1030), Clostridium autoethanogenum смеси для получения этанола и/или ацетата из (DSM ...

包含丁酸盐和选定发酵产物的饲料配料

提供了一种组合物，所述组合物包含a)丁酸盐和b)用天然产丁酸的梭菌纲细菌对含碳源的原料进行发酵的副产物。还提供了包含该组合物的饲料配料、动物饲料和防结冰产品、其制备方法和用途。

Bacteria for degrading ethylene oxide and applications thereof

A Kurthia gibsonii strain EO-06 with Deposit Number of CGMCC No. 18436, a Clostridium kogasensis strain EO-08 with Deposit Number of CGMCC No. 18438 and a Clostridium acidisoli strain EO-09 with Deposit Number of CGMCC No. 18439 are provided. The above strains can be used to treat pollution, for example, to treat industrial gas or wastewater containing ethylene oxide, which greatly improves the decontamination disposal capacity of ethylene oxide in industrial production. The present disclosure also provides a degradation agent for degrading ethylene oxide and a method for biodegrading ethylene oxide.

Method for producing urolithins

Method of producing higher alcohols

FIELD: biotechnology. SUBSTANCE: inventions relate to biotechnology. Disclosed are a reaction mixture for producing at least one high alcohol in an aqueous medium, a method of producing at least one higher alcohol in an aqueous medium, using said reaction medium to obtain at least one higher alcohol. Reaction mixture contains a mixed culture of an acetogenic microorganism selected from Clostridium ljungdahlii and Clostridium autoethanogenum, and Clostridium kluyveri in an aqueous medium containing gaseous carbon monoxide. Method envisages production of at least one higher alcohol in an aqueous medium containing said reaction mixture. EFFECT: inventions provide one-stage production of higher alcohols in the presence of carbon monoxide. 14 cl, 4 tbl, 10 ex РОССИЙСКАЯ ФЕДЕРАЦИЯ (19) RU (11) (13) 2 709 952 C2 (51) МПК C12N 1/20 (2006.01) C12P 7/02 (2006.01) C12P 7/06 (2006.01) C12P 7/18 (2006.01) C12R 1/145 (2006.01) ФЕДЕРАЛЬНАЯ СЛУЖБА ПО ИНТЕЛЛЕКТУАЛЬНОЙ СОБСТВЕННОСТИ (12) ОПИСАНИЕ ИЗОБРЕТЕНИЯ К ПАТЕНТУ (52) СПК C12N 1/20 (2019.05); C12P 7/02 (2019.05); C12P 7/06 (2019.05); C12P 7/065 (2019.05); C12P 7/18 (2019.05); C12R 1/145 (2019.05) (21)(22) Заявка: 2016102684, 27.01.2016 27.01.2016 Дата регистрации: (73) Патентообладатель(и): Эвоник Оперейшенс ГмбХ (DE) 23.12.2019 28.01.2015 EP 15152867.6 (43) Дата публикации заявки: 01.08.2017 Бюл. № 22 Адрес для переписки: 123242, Москва, Кудринская площадь, 1, а/я 35, "Михайлюк, Сороколат и партнеры-патентные поверенные" R U (54) СПОСОБ ПОЛУЧЕНИЯ ВЫСШИХ СПИРТОВ (57) Реферат: Изобретения относятся к биотехнологии. autoethanogenum, и Clostridium kluyveri в водной Предложены реакционная смесь для получения среде, содержащей газообразный монооксид по меньшей мере одного высшего спирта в углерода. Способ предусматривает получение по водной среде, способ получения по меньшей мере меньшей мере одного высшего спирта в водной одного высшего спирта в водной среде, среде, содержащей указанную реакционную смесь. применение указанной ...

Novel Strain of Clostridium sp. AWRP and Method for Selectively Producing Bio-alcohol Using the Same

본 발명은 클로스트리디움 속 신균주 AWRP 및 이를 이용한 합성 가스에서 바이오 알코올의 선택적 생산방법에 관한 것이다. 본 발명의 클로스트리디움 속 AWRP(수탁번호: KCTC 13908BP) 균주는 배양기간 동안 아세트산 축적으로 인한 세포 생장 저해가 일어나지 않고, 고농도의 바이오 에탄올의 생산이 가능하다. 따라서, 본 발명의 클로스트리디움 속 신균주 AWRP를 이용하여 합성 가스로부터 바이오 에탄올을 선택적으로 생산하는 공정에 유용하게 이용될 수 있다. The present invention relates to a new strain AWRP of the genus Clostridium and a method for selectively producing bioalcohol from synthesis gas using the same. Clostridium AWRP (Accession No.: KCTC 13908BP) strain of the present invention does not inhibit cell growth due to acetic acid accumulation during the culture period, and high concentration of bioethanol can be produced. Therefore, it can be usefully used in the process of selectively producing bioethanol from synthesis gas using the new strain AWRP of the genus Clostridium of the present invention.

Clostridiumm ljungdahlii, an anaerobic ethanol and acetate producing microorganism

A newly discovered microorganism was isolated in a biologically pure culture and designated Clostridium ljungdahlii, having the identifying characteristics of ATCC No. 49587. Cultured in an aqueous nutrient medium under anaerobic conditions, this microorganism is capable of producing ethanol and acetate from CO and H 2 O and/or CO 2 and H 2 in synthesis gas. Under optimal growth conditions, the microorganism produces acetate in preference to ethanol. Conversely, under non-growth conditions, ethanol production is favored over acetate.

Novel Clostridium sp. strain producing hexanoic acid in high yield and method for producing hexanoic acid using the same

The present specification relates to a Clostridium sp. strain JS66 that produces a metabolite with 4-6 carbon atoms at a high production yield. The strain reduces by-product production of acetic acid and ethanol and has a significantly high production yield of the C6 metabolite.

Isolation and characterization of novel clostridial species

A novel clostridia bacterial species ( Clostridium ragsdalei , ATCC BAA-622, “P11”) is provided. P11 is capable of synthesizing, from waste gases, products which are useful as biofuel. In particular, P11 can convert CO to ethanol. Thus, this novel bacterium transforms waste gases (e.g. syngas and refinery wastes) into useful products. P11 also catalyzes the production of acetate.

ISOLATION AND CHARACTERISTICS OF A NEW TYPE OF CLOSTRIDIUM

Обеспечивается новый бактериальный вид клостридий (Clostridium ragsdalei, ATCC BAA-622 и/или PTA-7826, «P11»). P11 способен синтезировать из газообразных отходов продукты, которые пригодны в качестве биологического топлива. В частности, P11 может превращать CO в этанол. Таким образом, эта новая бактерия трансформирует газообразные отходы (например, синтетический газ и нефтезаводские отходы) в полезные продукты. P11 также катализирует продукцию ацетата. A new bacterial species of Clostridia is provided (Clostridium ragsdalei, ATCC BAA-622 and / or PTA-7826, "P11"). P11 is capable of synthesizing products from waste gases that are suitable as biofuels. In particular, P11 can convert CO to ethanol. Thus, this new bacterium transforms waste gases (like syngas and refinery waste) into useful products. P11 also catalyzes the production of acetate.

Isolation and characterization of novel clostridial species

提供新的梭菌(Clostridium ragsdalei，ATCC BAA－622和/或PTA－7826，“P11”)。P11能由废气合成可用作生物燃料的产物。特别地，P11可将CO转化为乙醇。因此该新的细菌将废气(例如合成气和炼油厂废料废气)转化为有用产物。P11还催化醋酸盐的产生。

Isolation and characterization of novel clostridial species

A novel clostridia bacterial species (Clostridium ragsdalei, ATCC BAA-622 and/or PTA-7826, "P11") is provided. P11 is capable of synthesizing, from waste gases, products which are useful as biofuel. In particular, P11 can convert CO to ethanol. Thus, this novel bacterium transforms waste gases (e.g. syngas and refinery wastes) into useful products. P11 also catalyzes the production of acetate.

Isolation and characterization of novel clostridial species

Isolation and characterization of novel clostridial species.

Se proporciona una especie bacteriana clostridial novedosa (Clostridium ragsdalei, ATCC BAA-622 y/o PTA-7826, "P11"). P11 es capaz de sintetizar, a partir de gases de desecho, productos que son útiles como biocombustible. En particular, P11 puede convertir CO a etanol. Así, esta bacteria novedosa transforma los gases de desecho (por ejemplo signas y desechos de refinería) en productos útiles. P11 también cataliza la producción de acetato.

Isolation and characterization of novel clostridial species

A novel clostridia bacterial species (Clostridium ragsdalei, ATCC BAA-622 and/or PTA-7826, 'P11') is provided. P11 is capable of synthesizing, from waste gases, products which are useful as biofuel. In particular, P11 can convert CO to ethanol. Thus, this novel bacterium transforms waste gases (e.g. syngas and refinery wastes) into useful products. P11 also catalyzes the production of acetate.

Biologically pure culture of clostridium ragsdalei microorganisms, and composition, method and system for producing ethanol

CULTURA BIOLOGICAMENTE PURA DE MICROORGANISMO CLOSTRIDIUM RAGSDALEI, E, COMPOSIÇçO, MÉTODO E SISTEMA PARA PRODUZIR ETANOL. Uma nova espécie bacteriana clostridiana (Clostridium ragsdalei, ATCC BAA-622 e/ou PTA-7826, "P11") é provida. P11 é capaz de sintetizar, a partir de gases de refugo, produtos que utilizáveis como biocombustível. Em particular, P11 pode converter CO em etanol. Assim, esta nova bactéria transforma gases de refugo (por exemplo singás e refugos de refinaria) em produtos úteis. P11 também catalisa a produção de acetato. BIOLOGICALLY CULTURE OF CLOSTRIDIUM RAGSDALEI MICROORGANISM, AND COMPOSITION, METHOD AND SYSTEM FOR PRODUCING ETHANOL. A new clostridial bacterial species (Clostridium ragsdalei, ATCC BAA-622 and / or PTA-7826, "P11") is provided. P11 is able to synthesize, from waste gases, products that can be used as biofuel. In particular, P11 can convert CO to ethanol. Thus, this new bacterium transforms waste gases (for example singas and refinery waste) into useful products. P11 also catalyzes acetate production.

Isolation and characterization of novel clostridial species

A novel clostridia bacterial species ( Clostridium ragsdalei, ATCC BAA-622/PTA-7826, “P11”) is provided. P11 is capable of synthesizing, from waste gases, products which are useful as biofuel. In particular, P11 can convert CO to ethanol. Thus, this novel bacterium transforms waste gases (e.g. syngas and refinery wastes) into useful products. P11 also catalyzes the production of acetate.

Novel Ethanologenic Clostridium species, Clostridium coskatii

A novel clostridia bacterial species ( Clostridium coskatii ATCC No. PTA-10522, “PS02”) is provided. Under anaerobic conditions C. coskatii can convert CO and/or H 2 and/or CO 2 to ethanol or acetate. Thus, this novel bacterium is capable of transforming waste gases (e.g. syngas and refinery wastes) into useful products.

A method of producing higher alcohols

The present invention relates to a reaction mixture and a method of producing at least one higher alcohol comprising a reaction mixture comprising a mixed culture of a first and a second microorganism in an aqueous medium comprising carbon monoxide gas, wherein - the first microorganism is an acetogenic microorganism capable of converting a carbon source to acetate and/or ethanol; and - the second microorganism is selected from the group consisting of Clostridium kluyveri, and C.Carboxidivorans capable of converting the acetate and/or ethanol to form an acid; wherein the first microorganism is further capable of converting the acid to the corresponding higher alcohol and the higher alcohol comprises at least 6 carbon atoms.

An aerobic method of producing alcohols

A reaction mixture for producing ethanol and/or acetate from a carbon source in aerobic conditions, wherein the mixture comprises - a first acetogenic microorganism in an exponential growth phase; - free oxygen; and - a second acetogenic microorganism in a stationary phase wherein the first and second acetogenic microorganism is capable of converting the carbon source to the acetate and/or ethanol.

Genetically modified acetogenic cell

There is provided an acetogenic microbial cell which is capable of producing at least one higher alcohol from a carbon source, wherein the acetogenic microbial cell is genetically modified to comprise an increased expression relative to its wild type cell of at least one enzyme, E8, a butyryl-CoA:acetate CoA transferase (cat3). There is also provided a method and use of the cell to produce higher alcohols.

Method for producing higher linear fatty acids or esters

The present invention relates to a method of producing linear fatty acids comprising 7 to 28 carbon atoms or esters thereof using a combined biotechnological and chemical method. In particular, the present invention relates to a method of producing dodecanoic acid (i.e. lauric acid), via higher alkanones, preferably 6-undecanone.

Aqueous compositions comprising 6-undecanol-esters

The invention relates to aqueous compositions comprising 6-undecanol-esters and methods for producing 6-undecanol-esters, as well as the use of 6-undecanol-esters in cosmetic applications.

Improved carbon capture in microbial fermentation of industrial gases to ethanol

Disclosed is a method of capturing carbon by microbial fermentation, the method comprising: i. receiving one or more intermittent or non-continuous gas streams (15) in a buffering means (16); ii. passing a substantially continuous gas stream (17) from the buffering means to a bioreactor (5) containing a culture of one or more micro-organisms; and iii. fermenting the culture in the bioreactor to produce one or more products. Particularly preferred embodiments utilise a waste gas stream from a steel making process which contains carbon monoxide, the micro-organism is preferably Clostridium autoethanogenum and the product is ethanol.

bacteria and methods for their use

Alcohol production process

The invention relates to methods for improving the efficiency of carbon capture in microbial fermentation of a gaseous substrate comprising CO and/or H2; said method comprising applying an electrical potential across the fermentation. In certain aspects the invention relates to improving the efficiency of carbon capture in the microbial fermentation of gaseous substrate comprising CO and/or H2 to produce alcohol(s) and/or acid (s). In particular the invention relates to methods for improving the efficiency of carbon capture in carboxydotrophic fermentation.

Novel bacteria and methods of use thereof

This invention relates generally to the field of microbial fermentation of gases. It more particularly relates to a novel strain of

Recombinant microorganism and methods of production thereof

A novel genetically modified microorganisms capable of using CO to produce 1-butanol and/or a precursor thereof, novel methyltransferases and nucleic acids encoding same, methods for producing genetically modified microorganisms using said novel methyltransferases, and methods of producing 1-butanol and/or a precursor thereof by microbial fermentation.

Process for culturing microorganisms on a selected substrate

A process is provided that is effective for allowing bacteria to be cultured in a selected substrate. Bacteria are sporulated and then germinated in the presence of a selected substrate and medium.

Recombinant microorganisms and uses thereof

Recombinant microorganisms that make biodiesel

A carboxydotrophic acetogenic recombinant microorganism is modified so that it produces biodiesel and optionally one or more other products by fermentation of a substrate comprising CO. Biodiesel is produced by microbial fermentation of a substrate comprising CO. The recombinant microorganism is modified to express one or more exogenous enzymes in the biodiesel biosynthesis pathway not present in a parental microorganism from which the recombinant microorganism is derived. The one or more enzymes comprise a nonspecific acyltransferase.

Acidogenic clostridia and processes of using thereof for producing volatile fatty acids

An isolated biologically pure culture of Clostridium tyrobutyricum ITRI04001 or an isolated biologically pure culture of Clostridium tyrobutyricum having the genotypic characteristics of ITRI04001 useful for syngas fermentation. Volatile free acids are produced by a method comprising culturing a microorganism having the genotypic characteristics of ITRI04001 in a medium; providing at least one substrate comprising at least one carbon source chosen from CO and CO 2 to the microorganism; and recovering at least one free volatile free acid. Syngas can be the at least one substrate in these processes for producing volatile free acids.

Process for fermenting co-containing gaseous substrates in a low phosphate medium effective for reducing water usage

A process is provided for fermenting CO-containing gaseous substrates in a low phosphate medium. The process includes blending a liquid medium that includes at least one transition metal element with a liquid medium that includes at least at least one other transition metal element and one non-metal element to provide a fermentation medium. The process is effective for preventing precipitation of one or more transition metal elements with one or more non-metal elements. The fermentation medium used in the process is prepared in a way that requires significantly lower amounts of water and reduced levels of phosphate.

Recombinant microorganisms and how to use them

Compositions and methods for the conversion of short-chained carboxylic acids to alcohols using clostridial enzymes

The invention relates to the fields of bacterial metabolism and the utilization or consumption of short-chain carboxylic acids to reduced products. Specifically, it relates to syngas fermentations using monocultures of syngas-utilizing homoacetogenic bacteria for the production of alcohols using native alcohol dehydrogenase.

Anaerobic thermophilic culture system

A mixed culture system of the newly discovered microorganism Thermoanaerobacter ethanolicus ATCC31550 and the microorganism Clostridium thermocellum ATCC31549 is described. In a mixed nutrient culture medium that contains cellulose, these microorganisms have been coupled and cultivated to efficiently ferment cellulose to produce recoverable quantities of ethanol under anaerobic, thermophilic conditions.

Fermentation method producing ethanol

Ethanol is the major end product of an anaerobic, thermophilic fermentation process using a mutant strain of bacterium Clostridium thermosaccharolyticum. This organism is capable of converting hexose and pentose carbohydrates to ethanol, acetic and lactic acids. Mutants of Clostridium thermosaccharolyticum are capable of converting these substrates to ethanol in exceptionally high yield and with increased productivity. Both the mutant organism and the technique for its isolation are provided.

Mutant strain of C. acetobutylicum and process for making butanol

A biologically pure asporogenic mutant of Clostridium acetobutylicum is produced by growing sporogenic C. acetobutylicum ATCC 4259 and treating the parent strain with ethane methane sulfonate. The mutant which as been designated C. acetobutylicum ATCC 55025 is useful in an improved ABE fermentation process, and produces high concentrations of butanol and total solvents.

Clostridium stain which produces acetic acid from waste gases

A method and apparatus for converting waste gases from industrial processes such as oil refining, carbon black, coke, ammonia, and methanol production, into useful products. The method includes introducing the waste gases into a bioreactor where they are fermented to various organic acids or alcohols by anaerobic bacteria within the bioreactor. These valuable end products are then recovered, separated and purified. In an exemplary recovery process, the bioreactor raffinate is passed through an extraction chamber into which one or more non-inhibitory solvents are simultaneously introduced to extract the product. Then, the product is separated from the solvent by distillation. Gas conversion rates can be maximized by use of centrifuges, hollow fiber membranes, or other means of ultrafiltration to return entrained anaerobic bacteria from the bioreactor raffinate to the bioreactor itself, thus insuring the highest possible cell concentration.

Biological production of acetic acid from waste gases with Clostridium ljungdahlii

A method and apparatus for converting waste gases from industrial processes such as oil refining, carbon black, coke, ammonia, and methanol production, into useful products. The method includes introducing the waste gases into a bioreactor where they are fermented to various organic acids or alcohols by anaerobic bacteria within the bioreactor. These valuable end products are then recovered, separated and purified. In an exemplary recovery process, the bioreactor raffinate is passed through an extraction chamber into which one or more non-inhibitory solvents are simultaneously introduced to extract the product. Then, the product is separated from the solvent by distillation. Gas conversion rates can be maximized by use of centrifuges, hollow fiber membranes, or other means of ultrafiltration to return entrained anaerobic bacteria from the bioreactor raffinate to the bioreactor itself, thus insuring the highest possible cell concentration.

Biological production of ethanol from waste gases with Clostridium ljungdahlii

A method and apparatus for converting waste gases from industrial processes such as oil refining, carbon black, coke, ammonia, and methanol production, into useful products is disclosed. The method includes introducing the waste gases into a bioreactor where they are fermented to various product, such as organic acids, alcohols H 2 , SCP, and salts of organic acids by anaerobic bacteria within the bioreactor. These valuable end products are then recovered, separated and purified.

Biological production of products from waste gases

A method and apparatus are designed for converting waste gases from industrial processes such as oil refining, and carbon black, coke, ammonia, and methanol production, into useful products. The method includes introducing the waste gases into a bioreactor where they are fermented to various products, such as organic acids, alcohols, hydrogen, single cell protein, and salts of organic acids by anaerobic bacteria within the bioreactor. These valuable end products are then recovered, separated and purified.

Ethanologenic Clostridium species, Clostridium coskatii

An isolated clostridia bacterial species ( Clostridium coskatii ATCC No. PTA-10522, “PS02”) is provided. Under anaerobic conditions C. coskatii can convert CO and/or H 2 and/or CO 2 to ethanol or acetate. Thus, this bacterium is capable of transforming waste gases (e.g. syngas and refinery wastes) into useful products.

Ethanologenic Clostridium species, Clostridium coskatii

An isolated clostridia bacterial species ( Clostridium coskatii ATCC No. PTA-10522, “PS02”) is provided. Under anaerobic conditions C. coskatii can convert CO and/or H 2 and/or CO 2 to ethanol or acetate. Thus, this bacterium is capable of transforming waste gases (e.g. syngas and refinery wastes) into useful products.

An aerobic method of producing alcohols

A reaction mixture for producing ethanol and/or acetate from a carbon source in aerobic conditions, wherein the mixture comprises - a first acetogenic microorganism in an exponential growth phase; - free oxygen; and - a second acetogenic microorganism in a stationary phase wherein the first and second acetogenic microorganism is capable of converting the carbon source to the acetate and/or ethanol.

A method of producing higher alcohols

The present invention relates to a reaction mixture and a method of producing at least one higher alcohol comprising a reaction mixture comprising a mixed culture of a first and a second microorganism in an aqueous medium comprising carbon monoxide gas, wherein - the first microorganism is an acetogenic microorganism capable of converting a carbon source to acetate and/or ethanol; and - the second microorganism is selected from the group consisting of Clostridium kluyveri, and C. Carboxidivorans capable of converting capable of converting the acetate and/or ethanol to form an acid; wherein the first microorganism is further capable of converting the acid to the corresponding higher alcohol and the higher alcohol comprises at least 6 carbon atoms.

Aerobic method of producing alcohols

A reaction mixture for producing ethanol and/or acetate from a carbon source in aerobic conditions, wherein the mixture comprises a first acetogenic microorganism in an exponential growth phase; free oxygen; and a second acetogenic microorganism in a stationary phase wherein the first and second acetogenic microorganism is capable of converting the carbon source to the acetate and/or ethanol.

Isolation and characterization of novel clostridial species

A novel clostridia bacterial species (Clostridium ragsdalei, ATCC BAA-622 and/or PTA-7826, 'P11') is provided. P11 is capable of synthesizing, from waste gases, products which are useful as biofuel. In particular, P11 can convert CO to ethanol. Thus, this novel bacterium transforms waste gases (e.g. syngas and refinery wastes) into useful products. P11 also catalyzes the production of acetate.

Isolation and characterization of novel clostridial species

A novel clostridia bacterial species (Clostridium ragsdalei, ATCC BAA-622 and/or PTA-7826, 'P11') is provided. P11 is capable of synthesizing, from waste gases, products which are useful as biofuel. In particular, P11 can convert CO to ethanol. Thus, this novel bacterium transforms waste gases (e.g. syngas and refinery wastes) into useful products. P11 also catalyzes the production of acetate.

Novel ethanol producing bacterium and process for producing ethanol

A bacterium capable of producing ethanol at a temperature of 40°C or more from a carbon compound which is gaseous at ordinary temperature and ordinary pressure as the raw material, and a process for producing ethanol by culturing the bacterium at 40°C or more.

Butanol fermentation using acid pretreated biomass

本发明使用酸预处理的生物质（如麻风树籽饼、水黄皮籽饼及香蕉茎杆），作为通过突变体丙酮丁醇梭菌MTCC587生产丁醇的可再生原料。进行化学突变是为了改善菌株，使其具有更好的丁醇耐受性和产率。本发明首次报告了一种在单批发酵中获得高产率丁醇的工艺，该工艺使用酸预处理的麻风树籽饼。

Clostridium autoethanogenum strain and methods of use thereof to produce ethanol and acetate

A novel class of bacteria is described which has improved efficiency in the production of ethanol by anaerobic fermentation of substrates containing carbon monoxide. The exemplified bacterium,Clostridium autoethanogenum, is capable of producing ethanol and acetate at a ratio of at least 1Ø

biologically pure isolate of a bacterium clostridium autoethanogenum

BACTÉRIAS E MÉTODOS DE USO DAS MESMAS. A presente invenção refere-se geralmente ao campo da fermentação microbiana de gases. Mais particularmente, refere-se a uma nova classe de bactérias com eficiência melhorada na produção de etanol pela fermentação anaeróbica de substratos contendo monóxido de carbono (CO). BACTERIA AND METHODS OF USE OF THE SAME. The present invention generally relates to the field of microbial fermentation of gases. More particularly, it refers to a new class of bacteria with improved efficiency in ethanol production by anaerobic fermentation of substrates containing carbon monoxide (CO).