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Небесная энциклопедия

Космические корабли и станции, автоматические КА и методы их проектирования, бортовые комплексы управления, системы и средства жизнеобеспечения, особенности технологии производства ракетно-космических систем

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Мониторинг СМИ

Мониторинг СМИ и социальных сетей. Сканирование интернета, новостных сайтов, специализированных контентных площадок на базе мессенджеров. Гибкие настройки фильтров и первоначальных источников.

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02-02-2012 дата публикации

Yeast organism producing isobutanol at a high yield

Номер: US20120028323A1

Автор: Aristos Aristidou, Catherine Asleson Dundon, Christopher Smith, Jun URANO, Peter Meinhold, Reid M. Renny FELDMAN, Uvini GUNAWARDENA

Принадлежит: Gevo Inc

The present invention provides recombinant microorganisms comprising an isobutanol producing metabolic pathway and methods of using said recombinant microorganisms to produce isobutanol. In various aspects of the invention, the recombinant microorganisms may comprise a modification resulting in the reduction of pyruvate decarboxylase and/or glycerol-3-phosphate dehydrogenase activity. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.

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Номер записи: 1

04-04-2013 дата публикации

Native nad-dependent gapdh replaced with nadp-dependent gapdh plus nadk

Номер: US20130084600A1

Автор: George N. Bennett, Ka-Yiu San, Yipeng Wang

Принадлежит: William Marsh Rice University

This invention is metabolically engineer bacterial strains that provide increased intracellular NADPH availability for the purpose of increasing the yield and productivity of NADPH-dependent compounds. In the invention, native NAD-dependent GAPDH is replaced with NADP-dependent GAPDH plus overexpressed NADK. Uses for the bacteria are also provided.

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Номер записи: 2

18-07-2013 дата публикации

Fermentive production of four carbon alcohols

Номер: US20130183731A1

Автор: Andrew C. Eliot, Dennis Flint, Gail K. Donaldson, Lori Ann Maggio-Hall, Vasantha Nagarajan

Принадлежит: BUTAMAX ADVANCED BIOFUELS LLC

Methods for the fermentative production of four carbon alcohols is provided. Specifically, butanol, preferably isobutanol is produced by the fermentative growth of a recombinant bacterium expressing an isobutanol biosynthetic pathway.

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Номер записи: 3

29-08-2013 дата публикации

Recombinant microorganisms and uses therefor

Номер: US20130224838A1

Автор: Fungmin Liew, Michael Koepke, Sean Simpson, Wendy Chen

Принадлежит: Individual

The invention provides, inter alia, methods for the production of acetone, isopropanol and/or precursors of acetone and/or isopropanol by microbial fermentation of substrates comprising CO, genetically modified microorganisms of use in such methods, nucleic acids suitable for preparation of genetically modified microorganisms, a novel alcohol dehydrogenase and nucleic acids encoding same.

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Номер записи: 4

26-09-2013 дата публикации

Acetate supplemention of medium for butanologens

Номер: US20130252296A1

Автор: Lori Ann Maggio-Hall

Принадлежит: BUTAMAX ADVANCED BIOFUELS LLC

The invention relates to the fields of industrial microbiology and alcohol production. More specifically, the invention relates to improved production of butanol isomers by recombinant microorganisms containing an engineered butanol pathway and disrupted activity of the genes in pathways for the production of by-products during the fermentation when the microorganisms are grown in a fermentation medium containing acetate. In embodiments, recombinant microorganisms have an increased growth rate in a fermentation medium containing acetate as a C2 supplement.

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Номер записи: 5

12-12-2013 дата публикации

Recombinant microorganisms and uses therefor

Номер: US20130330809A1

Автор: Alexander Paul MUELLER, Michael Koepke, Shilpa NAGARAJU

Принадлежит: Lanzatech New Zealand Ltd

Carboxydotrophic acetogenic microorganisms do not produce MEK and/or 2-butanol. They lack the biosynthesis pathways to make these products. In addition, they produce the intermediate (R,R)-2,3-butanediol whereas the production of MEK and 2-butanol requires production of the intermediate (R,S)-2,3-butanediol. Nonetheless, the production of MEK and/or 2-butanol can be accomplished using recombinant microorganisms adapted to express or overexpress key enzymes in the MEK and/or 2-butanol biosynthesis pathways. Such microorganisms, such as the carboxydotrophic acetogen Clostridium autoethanogenum , can ferment substrates comprising CO. The overall scheme involves the production of 2-butanol from (R,S)-2,3-butanediol and the conversion of (R)-acetoin to (S)-2,3-butanediol. These steps are involved in the production of both MEK and 2-butanol. Such fermentation methods offer a means of using carbon monoxide from industrial processes which would otherwise be released into the atmosphere and pollute the environment.

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Номер записи: 6

26-12-2013 дата публикации

Engineered microbes and methods for microbial oil overproduction from cellulosic materials

Номер: US20130344548A1

Автор: Gregory Stephanopoulos, Mitchell Tai

Принадлежит: Massachusetts Institute of Technology

The invention relates to engineering microbial cells for utilization of cellulosic materials as a carbon source, including xylose.

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Номер записи: 7

13-02-2014 дата публикации

Construction of a lactobacillus casei ethanologen

Номер: US20140045235A1

Автор: James L. Steele, Jeff R. Broadbent

Принадлежит: WISCONSIN ALUMNI RESEARCH FOUNDATION

An engineered bacterium for producing ethanol from one or more carbohydrates is disclosed. The bacterium can be made by (a) inactivating within a Lactobacillus casei bacterium one or more endogenous genes encoding a lactate dehydrogenase; or (b) introducing into a Lactobacillus casei bacterium one or more exogenous genes encoding a pyruvate decarboxylase and one or more exogenous genes encoding an alcohol dehydrogenase II; or (c) performing both steps (a) and (b). The resulting engineered bacterium produces significantly more ethanol than the wild-type Lactobacillus casei bacterium, and can be used in producing ethanol from a substrate such as biomass that includes carbohydrates.

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Номер записи: 8

20-02-2014 дата публикации

Fermentive Production of Four Carbon Alcohols

Номер: US20140051151A1

Автор: Andrew C. Eliot, Dennis Flint, Gail K. Donaldson, Lori Ann Maggio-Hall, Vasantha Nagarajan

Принадлежит: BUTAMAX ADVANCED BIOFUELS LLC

Methods for the fermentative production of four carbon alcohols is provided. Specifically, butanol, preferably isobutanol is produced by the fermentative growth of a recombinant bacterium expressing an isobutanol biosynthetic pathway.

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Номер записи: 9

06-01-2022 дата публикации

Construction and Application of Engineered Strain of Escherichia Coli for Producing Malic Acid by Fixing CO2

Номер: US20220002766A1

Автор: Danlei MA, Guipeng HU, Jia Liu, Liming Liu, Qiuling Luo, Xiulai CHEN

Принадлежит: JIANGNAN UNIVERSITY

The disclosure discloses construction and application of an engineered strain of E. coli for producing malic acid by fixing CO2, and belongs to the field of fermentation. The engineered strain is obtained by performing genetic engineering transformation on Escherichia coli MG1655; the genetic engineering transformation includes knocking out a fumarate reductase gene, a fumarase gene, a lactate dehydrogenase gene and an alcohol dehydrogenase gene and freely overexpressing a formate dehydrogenase, an acetyl coenzyme A synthetase, an acylated acetaldehyde dehydrogenase, a formaldehyde lyase, a dihydroxyacetone kinase, a malic enzyme and a phosphite oxidoreductase to obtain a strain GH0407. The strain is used for producing malic acid by fermentation, anaerobic fermentation is performed for 72 hours with CO2 and glucose as a co-substrate, the production of malic acid reaches 39 g/L, the yield is 1.53 mol/mol, and accumulation of malic acid in the original strain is not achieved.

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Номер записи: 10

07-01-2016 дата публикации

YEAST HAVING IMPROVED PRODUCTIVITY AND METHOD OF PRODUCING PRODUCT

Номер: US20160002678A1

Автор: Cho Kwangmyung, Hahn Jisook, Kang Changduk, KIM Daehee, LEE SEUNGHYUN, LEE Sunghaeng, Song Jiyoon

Принадлежит:

A recombinant yeast cell capable of consuming glucose at an increased rate, and a method of efficiently producing glycolysis-derived products using the recombinant yeast cell. 1. A recombinant yeast cell having increased activity of at least one of GCR1 and GCR2 , wherein the recombinant yeast cell comprises a genetic modification that increases activity of at least one of GCR1 and GCR2 , in comparison with a yeast cell of the same type that does not comprise the genetic modification that increases activity of at least one of GCR1 and GCR2.2. The recombinant yeast cell of claim 1 , wherein the yeast cell is capable of consuming glucose at an increased glucose consumption rate in comparison with a yeast cell of the same type that does not comprise the genetic modification that increases activity of at least one of GCR1 and GCR2.3. The recombinant yeast cell of claim 1 , wherein the yeast cell has an increased productivity of a glycolysis intermediate or glycolysis intermediate-derived material in comparison with a yeast cell of the same type that does not comprise the genetic modification that increases activity of at least one of GCR1 and GCR2.4. The recombinant yeast cell of claim 3 , wherein the glycolysis intermediate comprises dihydroxyacetone phosphate (DHAP) claim 3 , glyceraldehyde 3-phosphate (GAP) claim 3 , or pyruvate claim 3 , and wherein the glycolysis intermediates-derived material comprises glyceol-3-phosphate (G3P) claim 3 , glycerol claim 3 , acetyl-CoA claim 3 , ethanol claim 3 , acetic acid claim 3 , lactate claim 3 , citric acid claim 3 , itaconic acid claim 3 , isocitric acid claim 3 , oxalosuccinic acid claim 3 , α-ketoglutaric acid claim 3 , succinic acid claim 3 , succinyl-CoA claim 3 , fumaric acid claim 3 , maleic acid claim 3 , oxaloacetic acid claim 3 , 1 claim 3 ,3-butanediol (1 claim 3 ,3-BDO) claim 3 , 1 claim 3 ,4-butanediol (1 claim 3 ,4-BDO) claim 3 , butanol claim 3 , isobutanol claim 3 , or a combination thereof.5. The recombinant ...

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Номер записи: 11

05-01-2017 дата публикации

Recombinant microorganism having enhanced d(-) 2,3-butanediol producing ability and method for producing d(-) 2,3-butanediol using the same

Номер: US20170002384A1

Автор: Taek-Ho Yang

Принадлежит: GS Caltex Corp

The present invention relates to a recombinant microorganism for producing D(−) 2,3-butanediol, wherein a gene encoding an enzyme for converting acetoin into D(−) 2,3-butanediol is introduced into a microorganism having a pathway for converting acetoin into 2,3-butanediol. In addition, the present invention relates to a method for producing D(−) 2,3-butanediol by using the recombinant microorganism.

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Номер записи: 12

07-01-2021 дата публикации

MODIFIED HOMOSERINE DEHYDROGENASE AND METHOD FOR PRODUCING HOMOSERINE OR L-AMINO ACID DERIVED FROM HOMOSERINE USING THE SAME

Номер: US20210002682A1

Автор: Huh Lan, KIM Hyo Jin, Kim Hyoung Joon, Kim Hyun Ah, LEE Ji Sun, LEE Seung Bin, Lim Sang Jo, Seo Chang Il

Принадлежит:

The present disclosure relates to modified homoserine dehydrogenase and a method for producing a homoserine-derived L-amino acid using the same. 1. A modified homoserine dehydrogenase , wherein in the amino acid sequence of SEQ ID NO: 1 , the amino acid at position 285 is substituted with isoleucine; the amino acid at position 398 is substituted with glutamine; or the amino acids at both positions are substituted with isoleucine and glutamine , respectively.2. The modified homoserine dehydrogenase according to claim 1 , wherein in the amino acid sequence of SEQ ID NO: 1 claim 1 , the amino acid at position 378 is further substituted with tryptophan.3. A polynucleotide encoding the modified homoserine dehydrogenase of .4Corynebacterium,. A microorganism of the genus comprising the modified homoserine dehydrogenase of .5CorynebacteriumCorynebacterium. The microorganism according to claim 4 , wherein the microorganism of the genus is a microorganism of the genus producing homoserine or a homoserine-derived L-amino acid.6. The microorganism according to claim 5 , wherein the homoserine-derived L-amino acid is at least one kind selected from the group consisting of L-threonine claim 5 , L-isoleucine claim 5 , O-acetyl homoserine claim 5 , and L-methionine.7Corynebacterium. The microorganism according to claim 4 , wherein the microorganism of the genus produces L-alanine.8CorynebacteriumCorynebacterium glutamicum.. The microorganism according to claim 4 , wherein the microorganism of the genus is9. A method for producing homoserine or a homoserine-derived L-amino acid claim 4 , comprising:{'claim-ref': {'@idref': 'CLM-00004', 'claim 4'}, 'culturing a microorganism of in a medium; and'}recovering homoserine or a homoserine-derived L-amino acid from the microorganism or medium.10. The method according to claim 9 , wherein the homoserine-derived L-amino acid is at least one kind selected from the group consisting of L-threonine claim 9 , L-isoleucine claim 9 , O-acetyl ...

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Номер записи: 13

12-01-2017 дата публикации

MICROORGANISMS THAT CO-CONSUME GLUCOSE WITH NON-GLUCOSE CARBOHYDRATES AND METHODS OF USE

Номер: US20170009262A1

Автор: Kim Joonhoon, Reed Jennifer L.

Принадлежит:

Microorganisms that co-consume glucose with non-glucose carbohydrates, such as xylose, and methods of using same. The microorganisms comprise modifications that reduce or ablate the activity of a phosphoenolpyruvate (PEP):carbohydrate phosphotransferase system (PTS) protein or modifications that reduce or ablate the activity of a phosphoglucose isomerase and a GntR. The PTS protein may be selected from an enzyme I (EI), an HPr, an FPr, and an enzyme II(EII). Additional modifications include reduction or ablation of the activity of a pyruvate formate lyase, a lactate dehydrogenase, and a fumarate reductase and inclusion of recombinant pyruvate decarboxylase and alcohol dehydrogenase genes. The microorganisms are particularly suited to co-consuming glucose and xylose in media containing these substrates and producing ethanol therefrom. 1. A recombinant microorganism comprising:{'sup': Glc', 'Glc, 'modifications that reduce or ablate the activity of a phosphoenolpyruvate (PEP):carbohydrate phosphotransferase system (PTS) protein selected from the group consisting of an enzyme I (EI), an HPr, an FPr, and an enzyme II(EII); or'}a first modification that reduces or ablates the activity of a phosphoglucose isomerase and a second modification selected from the group consisting of a modification that reduces or ablates the activity of a GntR, a modification that introduces a recombinant phosphogluconate dehydratase gene, a modification that introduces a recombinant 2-keto-4-hydroxyglutarate aldolase gene, a modification that introduces a recombinant 2-keto-3-deoxy-6-phosphogluconate aldolase gene, and a modification that introduces a recombinant oxaloacetate decarboxylase gene.2. The recombinant microorganism of wherein the microorganism is a bacterium.3E. coliE. coli. The recombinant microorganism of comprising a modification that reduces or ablates the activity of HPr of or an ortholog thereof and FPr of or an ortholog thereof.4. The recombinant microorganism of further ...

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Номер записи: 14

11-01-2018 дата публикации

GENETICALLY MODIFIED MICROORGANISMS HAVING IMPROVED TOLERANCE TOWARDS L-SERINE

Номер: US20180010157A1

Автор: Mundhada Hemanshu, Nielsen Alex Toftgaard

Принадлежит: DANMARKS TEKNISKE UNIVERSITET

The present invention generally relates to the microbiological industry, and specifically to the production of L-serine or L-serine derivatives using genetically modified bacteria. The present invention provides genetically modified microorganisms, such as bacteria, wherein the expression of genes encoding for enzymes involved in the degradation of L-serine is attenuated, such as by inactivation, which makes them particularly suitable for the production of L-serine at higher yield. The present invention also provides means by which the microorganism, and more particularly a bacterium, can be made tolerant towards higher concentrations of serine. The present invention also provides methods for the production of L-serine or L-serine derivative using such genetically modified microorganisms. 1. A bacterium , which has been modified to attenuate expression of at least one gene coding for a polypeptide comprising serine deaminase activity and/or to attenuate expression of a gene coding for a polypeptide comprising serine hydroxymethyltransferase activity.235-. (canceled)36. The bacterium according to claim 1 , wherein the at least one gene coding for a polypeptide comprising serine deaminase activity is selected from the group consisting of sdaA claim 1 , sdaB and tdcG.37. The bacterium according to claim 1 , wherein the gene coding for a polypeptide comprising serine hydroxymethyltransferase activity is glyA.38. The bacterium according to claim 1 , wherein the bacterium has been modified to attenuate expression of the genes sdaA claim 1 , sdaB claim 1 , tdcG and glyA.39. The bacterium according to claim 1 , wherein the bacterium has been modified to attenuate expression of at most three of the genes sdaA claim 1 , sdaB claim 1 , tdcG and glyA.40. The bacterium according to claim 1 , wherein the expression of the gene or genes is attenuated by inactivation of the gene or genes.41. The bacterium according to claim 1 , wherein at least one gene coding for a polypeptide ...

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Номер записи: 15

09-01-2020 дата публикации

SACCHAROMYCES CEREVISIAE STRAINS

Номер: US20200010793A1

Автор: Bonander Nicklas

Принадлежит:

The present invention relates to a method of preparing a strain of sugar fermenting with capability to ferment xylose, wherein said method comprises different procedural steps. The method comprises mating a first sporulated strain with a second haploid strain. Thereafter, screening for mated cells is performed, growing such mated cells, and verifying that mated cells exhibit basic morphology by microscopic inspection. Thereafter, creation of a mixture of the mated cells is performed, subjecting the mixture to continuous chemostat lignocellulose cultivation and obtaining the sugar fermenting cells with capability to ferment xylose is performed. The invention also comprises strains obtained by said method. 1Saccharomyces cerevisiae. A method of preparing a strain of comprising at least one native XKS1 gene in its genome encoding xylulokinase , at least one native XDH1 gene in its genome encoding xylitol dehydrogenase , and at least one modGre3 gene in its genome , said modGre3 gene encoding an amino acid sequence of SEQ ID NO 1 having xylose reductase activity or encoding a fragment of said amino acid sequence having xylose reductase activity , wherein said method comprises the following steps:{'i': 'Saccharomyces cerevisiae', 'a) sporulating a first strain of for providing at least 20 tetrads of said strain,'}{'i': Scheffersomyces stipitis', 'Saccharomyces cerevisiae', 'Saccharomyces cerevisiae,, 'b) introducing DNA, encoding for xylose reductase and xylitol dehydrogenase obtained from and xylulokinase obtained from , into a second strain of'}{'i': Saccharomyces cerevisiae', 'Saccharomyces cerevisiae', 'Saccharomyces cerevisiae, 'c) mating the first sporulated strain with the second strain evolved on xylose and in a haploid state by mixing cells of said haploid strain with each tetrad obtained in step a) to provide mated cells on an YPD agar plate,'}d) screening for mated cells on xylose and geneticin agar plates,e) growing mated cells from step d) in minimal defined ...

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Номер записи: 16

09-01-2020 дата публикации

Trichothecene-transforming alcohol dehydrogenase, method for transforming trichothecenes and trichothecene-transforming additive

Номер: US20200010812A1

Автор: Barbara Weber, Claudia BERNARD, Eva-Maria Binder

Принадлежит: Erber AG

An alcohol dehydrogenase of sequence ID numbers 2, 3 or 4 containing metal ions and a quinone cofactor, or in addition, a functional variant exhibiting a sequence identity of at least 80%, preferably at least 86%, especially preferred at least 89% and at least one redox cofactor for the transformation of at least one trichothecene exhibiting a hydroxyl group on the C-3 atom, as well as a method for the enzymatic transformation of trichothecenes and a trichothecene-transforming additive.

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Номер записи: 17

09-01-2020 дата публикации

LONG-CHAIN DIBASIC ACID WITH LOW CONTENT OF HYDROXYL ACID IMPURITY AND PRODUCTION METHOD THEREOF

Номер: US20200010855A1

Автор: Chou Howard, Liu Wenbo, LIU Xiucai, XU Min, Yang Chen

Принадлежит:

The present invention relates to a long-chain dibasic acid with low content of hydroxyl acid impurity and a production method thereof, in particular to a method for producing a long-chain dibasic acid with low content of hydroxyl acid impurity by fermenting a long-chain dibasic acid producing strain prepared by homologous recombination method. The present invention relates to a recombinant long-chain dibasic acid producing microorganism, having increased alcohol dehydrogenase activity and optionally decreased acetyl-CoA oxidase activity. The present invention also relates to a method of producing a long-chain dibasic acid by the recombinant long-chain dibasic acid producing microorganism and use thereof. 1. A product , which is one of the following products I) to III):I) a recombinant long-chain dibasic acid producing microorganism, having increased alcohol dehydrogenase activity and optionally decreased acetyl-CoA oxidase activity;II) a long-chain dibasic acid with low content of hydroxyl acid impurity, wherein the content of the hydroxyl acid impurity in the long-chain dibasic acid is more than 0 and less than 10,000 ppm, 4,000 ppm, 300 ppm or less, wherein the hydroxyl acid impurity comprises a hydroxyl fatty acid having one carboxyl group;III) a fermentation broth in a process for producing a long-chain dibasic acid by fermentation with a microorganism, wherein the fermentation broth contains hydroxyl acid impurity, wherein the content of the hydroxyl acid impurity is less than 3%, 2%, 1.5%, 1.3%, 1.2%, 1.1%, 1.0%, 0.9%, 0.8%, 0.7%, 0.6%, 0.5%, 0.4%, 0.3% or less, wherein the percentage is the mass percentage of the hydroxyl acid impurity to the long-chain dibasic acid in the fermentation broth.2. The product of claim 1 , which is I) the recombinant long-chain dibasic acid producing microorganism claim 1 , wherein the recombinant long-chain dibasic acid producing microorganism:(i) contains an overexpressed ADH gene or its homologous gene and/or an attenuated ...

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Номер записи: 18

15-01-2015 дата публикации

RECOMBINANT HOST CELLS AND PROCESSES FOR PRODUCING 1,3-BUTADIENE THROUGH A CROTONOL INTERMEDIATE

Номер: US20150017696A1

Автор: Agard Nicholas J., Davis Simon Christopher, Grate John H.

Принадлежит:

The present disclosure relates to recombinant host cells comprising one or more recombinant polynucleotides encoding enzymes in select pathways that provide the ability to use the cells to produce 1,3-butadiene. The present disclosure also provides methods of manufacturing the recombinant host cells, and methods for the use of the cells to produce 1,3-butadiene, either through formation of the intermediate compound crotonol followed by chemo-catalytic dehydration to 1,3-butadiene, or through the use of a recombinant cell comprising a fully enzymatic pathway capable of converting crotonyl-CoA or crotonyl-ACP to crotonol and then crotonol to 1,3-butadiene. 1. A recombinant host cell capable of producing crotonol , the host cell comprising:(a) a recombinant polynucleotide encoding a FAR enzyme capable of converting crotonyl-CoA and/or crotonyl-ACP to crotonol.2. The recombinant host cell of claim 1 , wherein the host cell further is capable of producing 1 claim 1 ,3-butadiene and further comprises:(b) a recombinant polynucleotide encoding an enzyme capable of converting crotonol to but-2-enyl phosphate; and(c) a recombinant polynucleotide encoding an enzyme capable of converting but-2-enyl phosphate to 1,3-butadiene.3. The recombinant host cell of claim 1 , wherein the recombinant polynucleotide encoding the FAR enzyme comprises one or more nucleotide sequence differences relative to the corresponding naturally occurring polynucleotide claim 1 , which result in an improved property selected from:(a) increased activity of the FAR enzyme in the conversion of crotonyl-CoA and/or crotonyl-ACP to crotonol;(b) increased expression of the FAR enzyme;(c) increased host cell tolerance of acetyl-CoA, acetoacetyl-CoA, malonyl-CoA, malonyl-ACP, 3-hydroxybutyryl-CoA, acetoacetyl-ACP, crotonyl-CoA, crotonyl-ACP, crotonol, but-2-enyl phosphate, or 1,3-butadiene; or(d) altered host cell concentration of acetyl-CoA, acetoacetyl-CoA, malonyl-CoA, malonyl-ACP, 3-hydroxybutyryl-CoA, ...

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Номер записи: 19

19-01-2017 дата публикации

TEST STRIP AND APPARATUS FOR MEASURING THE CONTENT OF ALCOHOL IN BLOOD, OR OF ANY OTHER SUBSTANCE IN BLOOD, AND A METHOD FOR MEASURING THE CONTENT OF ALCOHOL IN BLOOD

Номер: US20170016044A1

Автор: KOLEHMAINEN Kari, VARTIAINEN IIkka, VARTIAINEN Riikka

Принадлежит: PAL Finland Oy

A test strip for measuring the blood alcohol content from blood includes a base material and two electrodes attached to it and a reaction area connected to both electrodes, to which area nicotin—amide adenine dinucleotide (NAD) and alcohol dehydrogenase enzyme (ADH) are applied as reagents. A sample opening is connected to the reaction area, from which opening the blood sample to be measured can be transferred to the reaction area to dissolve the reagents and optional auxiliary substances, and at the same time into contact with both electrodes. Between the electrodes can thus be formed a potential difference which makes possible the movement of the protons (H) formed in the reaction towards a negatively charged working electrode, whereby a measurable change in current is formed. The invention further relates to an apparatus and method for measuring the blood alcohol content from blood. 1. A test strip for measuring the blood alcohol content from blood , the test strip comprising a base material and two electrodes , attached to it and a reaction area connected to both electrodes , to which reaction area nicotinamide adenine dinucleotide (NAD) and alcohol dehydrogenase enzyme (ADH) are applied as reagents , and optionally one or more auxiliary substances , a sample opening in connection with the reaction area , from which sample opening the blood sample to be measured can be transferred to the reaction area to dissolve the reagents and optional auxiliary substances , and at the same time into contact with both electrodes , between which can be formed a potential difference which makes possible the movement of the protons (H) formed in the reaction towards a negatively charged working electrode , whereby a measurable change in current is formed.2. The test strip as claimed in claim 1 , wherein the electrodes are graphite electrodes.3. The test strip as claimed in claim 1 , to the reaction area of which saccharose and bovine serum albumin (BSA) are applied as auxiliary ...

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Номер записи: 20

21-01-2016 дата публикации

METHODS FOR REGULATING NITROGEN METABOLISM DURING THE PRODUCTION OF ETHANOL FROM CORN BY METABOLICALLY ENGINEERED YEAST STRAINS

Номер: US20160017377A1

Автор: Argyros Aaron, Barrett Trisha

Принадлежит:

The present invention provides for a mechanism to reduce glycerol production and increase nitrogen utilization and ethanol production of recombinant microorganisms. One aspect of this invention relates to strains of with reduced glycerol productivity that get a kinetic benefit from higher nitrogen concentration without sacrificing ethanol yield. A second aspect of the invention relates to metabolic modifications resulting in altered transport and/or intracellular metabolism of nitrogen sources present in com mash.

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Номер записи: 21

18-01-2018 дата публикации

METHOD FOR THE PRODUCTION OF L-SERINE USING GENETICALLY ENGINEERED MICROORGANISMS DEFICIENT IN SERINE DEGRADATION PATHWAYS

Номер: US20180016546A1

Автор: Mundhada Hemanshu, Nielsen Alex Toftgaard

Принадлежит:

The present invention generally relates to the microbiological industry, and specifically to the production of L-serine using genetically modified bacteria. The present invention provides genetically modified microorganisms, such as bacteria, wherein the expression of genes encoding for enzymes involved in the degradation of L-serine is attenuated, such as by inactivation, which makes them particularly suitable for the production of L-serine at higher yield. The present invention also provides means by which the microorganism, and more particularly a bacterium, can be made tolerant towards higher concentrations of serine. The present invention also provides methods for the production of L-serine or L-serine derivative using such genetically modified microorganisms. 1. A bacterium , which has been modified to attenuate expression of genes coding for polypeptides comprising serine deaminase activity and to attenuate expression of a gene coding for a polypeptide comprising serine hydroxymethyltransferase activity.220-. (canceled)21. The bacterium according to claim 1 , wherein the expression of the genes sdaA claim 1 , sdaB claim 1 , tdcG and glyA is attenuated.22. The bacterium according to claim 1 , wherein the expression of the genes is attenuated by inactivation of the genes.23. The bacterium according to claim 1 , wherein said bacterium has been further modified to overexpress a 3-phosphoglycerate dehydrogenase claim 1 , a phosphoserine phosphatase and a phosphoserine aminotransferase.24. The bacterium according to claim 1 , wherein said bacterium is capable of growing in a minimal culture medium comprising L-serine at a concentration of at least about 6.25 g/L.25. The bacterium according to claim 1 , wherein said bacterium is capable of growing in a minimal culture medium comprising L-serine at a concentration of at least about 6.25 g/L at a growth rate of at least 0.1 hrduring exponential growth.26. The bacterium according to claim 1 , wherein said bacterium ...

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Номер записи: 22

21-01-2021 дата публикации

ANTI-MICROBIAL AND UV-PROTECTIVE EXTRACTS AND METHODS OF MAKING AND USING THEREOF

Номер: US20210017525A1

Автор: CUERO RENGIFO Raul, LONDONO MURILLO Juliana

Принадлежит:

Described herein are anti-microbial and UV-protective biological devices and extracts produced therefrom. The biological devices include microbial cells transformed with a DNA construct containing genes for producing proteins such as, for example, zinc-related protein/oxidase, silicatein, silaffin, and alcohol dehydrogenase. In some instances, the biological devices also include a gene for lipase. Methods for producing and using the devices are also described herein. Finally, compositions and methods for using the devices and extracts to kill microbial species or prevent microbial growth and to reduce or prevent UV-induced damage or exposure to materials, items, plants, and human and animal subjects are described herein. Also disclosed are biological devices producing polyactive carbohydrates and carbo sugars, as well as compositions and articles incorporating both extracts from these devices and the anti-microbial and UV-protective extracts. 1. A DNA construct comprising the following genetic components: (a) a gene that expresses zinc-related protein/oxidase , (b) a gene that expresses silicatein , (c) a gene that expresses silaffin , and (d) a gene that expresses alcohol dehydrogenase II.2. The DNA construct of claim 1 , further comprising (e) a gene that expresses lipase.3. The DNA construct of claim 1 , wherein the gene that expresses zinc-related protein/oxidase has SEQ ID NO. 1 or at least 70% homology thereto.4. The DNA construct of claim 1 , wherein the gene that expresses silicatein has SEQ ID NO. 2 or at least 70% homology thereto.5. The DNA construct of claim 1 , wherein the gene that expresses silaffin has SEQ ID NO. 3 or at least 70% homology thereto.6. The DNA construct of claim 1 , wherein the gene that expresses alcohol dehydrogenase II has SEQ ID NO. 4 or at least 70% homology thereto.7. The DNA construct of claim 2 , where in the gene that expresses lipase has SEQ ID NO. 7 or at least 70% homology thereto.8. The DNA construct of claim 1 , wherein ...

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Номер записи: 23

28-01-2016 дата публикации

Electro-autotrophic synthesis of higher alcohols

Номер: US20160024533A1

Автор: James C. Liao, Kwang Myung Cho

Принадлежит: UNIVERSITY OF CALIFORNIA

The disclosure provides a process that converts CO 2 to higher alcohols (e.g. isobutanol) using electricity as the energy source. This process stores electricity (e.g. from solar energy, nuclear energy, and the like) in liquid fuels that can be used as high octane number gasoline substitutes. Instead of deriving reducing power from photosynthesis, this process derives reducing power from electrically generated mediators, either H 2 or formate. H 2 can be derived from electrolysis of water. Formate can be generated by electrochemical reduction of CO 2 . After delivering the reducing power in the cell, formate becomes CO 2 and recycles back. Therefore, the biological CO 2 fixation process can occur in the dark.

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Номер записи: 24

25-01-2018 дата публикации

MATERIALS AND METHODS FOR THE BIOSYNTHESIS OF SEVEN CARBON CHEMICALS IN THE PRESENCE OF METHANOL OXIDATION

Номер: US20180023088A1

Автор: CHOKKATHUKALAM Achuthanunni, MOMO Remi Ako Mbianyor, VAN ECK CONRADIE Alex

Принадлежит:

This disclosure describes methods for regulating the biosynthesis of pimelic acid, 7-aminoheptanoate, 7-hydroxyheptanoate, heptamethylenediamine, 7-aminoheptanol, or 1,7-heptanediol by channeling increased flux through the biosynthesis pathway to obtain an intermediate required for growth of the host microorganism. 1. A method for regulating biosynthesis of a product chosen from pimelic acid , 7-aminoheptanoate , 7-hydroxyheptanoate , heptamethylenediamine , 7-aminoheptanol , and 1 ,7-heptanediol , or salts and derivatives thereof , using a pathway having a pimeloyl-ACP intermediate , the method comprising converting methanol to formate via at least one spontaneous enzymatic reaction , wherein the formate is used in the conversion of tetrahydrofolate to N-formyl-tetrahydrofolate.2. The method of claim 1 , wherein:the methanol is produced during BioH enzyme activity; and/orthe method comprises the step of downregulating the activity of FolD.3. The method of claim 2 , wherein:BioH removes the methyl group from pimeloyl-ACP methyl ester during conversion of pimeloyl-ACP methyl ester to pimeloyl-ACP; and/orthe step of downregulating the activity of FolD comprises a step of attenuating folD.4. (canceled)5. (canceled)6. The method of claim 3 , whereinthe method comprises the step of cloning in a formate-tetrahydrofolate ligase (fhs);the method comprises the step of downregulating the activity of PflB and TdcE;the method comprises a step of cloning in an alcohol dehydrogenase (adh);the method comprises a step of cloning in a S-(hydroxymethyl), glutathione dehydrogenase (frmA); and/orthe method comprises a step of cloning in a S-formylglutathione hydrolase (frmB).7. The method of claim 6 , wherein:the formate-tetrahydrofolate ligase has at least 70%, at least 80%, or at least 90% sequence identity or homology to an amino acid sequence chosen from SEQ ID NOs: 18-22;the alcohol dehydrogenase has at least 70%, at least 80%, or at least 90% sequence identity or homology to the ...

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Номер записи: 25

23-01-2020 дата публикации

IMPROVED GLYCEROL FREE ETHANOL PRODUCTION

Номер: US20200024619A1

Автор: DE BRUIJN Hans Marinus Charles Johannes, DE WAAL Paulus Petrus, SCHMITZ Jozef Petrus Johannes

Принадлежит:

The invention relates to a recombinant cell, preferably a yeast cell comprising one or more genes coding for an enzyme having glycerol dehydrogenase activity, one or more genes coding dihydroxyacetone kinase (E.C. 2.7.1.28 and/or E.C. 2.7.1.29); one or more genes coding for an enzyme in an acetyl-CoA-production pathway and one or more genes coding for an enzyme having at least NAD dependent acetylating acetaldehyde dehydrogenase activity (EC 1.2.1.10 or EC 1.1.1.2), and optionally one or more genes coding for a glycerol transporter. This cell can be used for the production of ethanol and advantageously produces little or no glycerol. 1. A recombinant cell , optionally a yeast cell , said recombinant cell comprising:one or more genes coding for an enzyme having glycerol dehydrogenase activity;one or more genes coding dihydroxyacetone kinase (E.C. 2.7.1.28 and/or E.C. 2.7.1.29);one or more genes coding for an enzyme in an acetyl-CoA-production pathway; and{'sup': '+', 'one or more genes coding for an enzyme having at least NAD dependent acetylating acetaldehyde dehydrogenase activity (EC 1.2.1.10 or EC 1.1.1.2); and optionally'}one or more genes coding for a glycerol transporter.2. The Cell according to wherein the enzyme having glycerol dehydrogenase activity is a NAD linked glycerol dehydrogenase (EC 1.1.1.6).3. The Cell according to wherein the enzyme having glycerol dehydrogenase activity is a NADP linked glycerol dehydrogenase (EC 1.1.1.72).4. The recombinant cell according to wherein the one or more genes coding for an enzyme in an acetyl-CoA-production pathway comprises:one or more genes coding for an enzyme having phosphoketolase (PKL) activity (EC 4.1.2.9 or EC 4.1.2.22) or an enzyme having an amino acid sequence according SEQ ID NO: 5, 6, 7, or 8, or functional homologues thereof having a sequence identity of at least 50%, and/orone or more genes coding for an enzyme having phosphotransacetylase (PTA) activity (EC 2.3.1.8) or an enzyme having an amino acid ...

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Номер записи: 26

23-01-2020 дата публикации

ALCOHOL BASED BIOFUEL CELL

Номер: US20200028194A1

Автор: Arugula Mary, Pinchon Erica Wagner, Singhal Sameer

Принадлежит:

An anode can include: an electrode substrate; a first region of the substrate having a catalyst composition located thereon, wherein the catalyst composition includes an inorganic or metallic catalyst; and a second region of the substrate having an enzyme composition located thereon, wherein the combination of the catalyst composition and enzyme composition converts a fuel reagent to carbon dioxide at neutral pH. The first region and second region can be separate regions. The catalyst of the catalyst composition can include gold nanoparticles. The catalyst can include an inorganic or metallic catalyst selected from vanadium oxide, titanium (III) chloride, Pd(OAc), MnO, zeolite, alumina, graphitic carbon, palladium, platinum, gold, ruthenium, rhodium, iridium, or combinations thereof. The catalyst can be nanoparticle, nanorod, nanodot, or combination thereof. The catalyst can have sizes that range from about 10 to 20 nm. 1. An anode comprising:an electrode substrate;a first region of the substrate having a catalyst composition located thereon, wherein the catalyst composition includes an inorganic or metallic catalyst; anda second region of the substrate having an enzyme composition located thereon, wherein the combination of the catalyst composition and enzyme composition converts a fuel reagent to carbon dioxide at neutral pH,wherein the first region and second region are separate regions.2. The anode of claim 1 , wherein the catalyst of the catalyst composition includes an inorganic or metallic catalyst selected from vanadium oxide claim 1 , titanium (III) chloride claim 1 , Pd(OAc) claim 1 , MnO claim 1 , zeolite claim 1 , alumina claim 1 , graphitic carbon claim 1 , palladium claim 1 , platinum claim 1 , gold claim 1 , ruthenium claim 1 , rhodium claim 1 , iridium claim 1 , or combinations thereof.3. The anode of claim 2 , wherein the catalyst is in the form of a nanoparticle claim 2 , nanorod claim 2 , nanodot claim 2 , or combination thereof.4. The anode of ...

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Номер записи: 27

02-02-2017 дата публикации

Fermentive Production of Four Carbon Alcohols

Номер: US20170029851A1

Автор: DONALDSON Gail K., ELIOT Andrew C., Flint Dennis, Maggio-Hall Lori Ann, Nagarajan Vasantha

Принадлежит:

Methods for the fermentative production of four carbon alcohols is provided. Specifically, butanol, preferably isobutanol is produced by the fermentative growth of a recombinant bacterium expressing an isobutanol biosynthetic pathway. 182-. (canceled)84. The method of claim 83 , further comprising recovering the bioproduced isobutanol.85. The method of claim 84 , further comprising removing solids from the fermentation medium.86. The method of claim 84 , wherein the recovering is by distillation claim 84 , liquid-liquid extraction claim 84 , adsorption claim 84 , decantation claim 84 , pervaporation claim 84 , or combinations thereof.87. The method of claim 85 , wherein the removing is by centrifugation claim 85 , filtration claim 85 , or decantation.88. The method of claim 85 , wherein the removing occurs before the recovering. This application is a continuation of and claims priority to U.S. patent application Ser. No. 14/715,992, filed May 19, 2015 which is a continuation of and claims priority to U.S. patent application Ser. No. 13/539,125, now U.S. Pat. No. 9,068,190, filed on Jun. 29, 2012 which is a continuation of and claims priority to U.S. patent application Ser. No. 12/939,284, now U.S. Pat. No. 8,283,144, filed on Nov. 4, 2010 which is a continuation of and claims priority to U.S. patent application Ser. No. 11/586,315, now U.S. Pat. No. 7,851,188, filed on Oct. 25, 2006, which claims priority under 35 U.S.C. §119 from U.S. Provisional Application Ser. No. 60/730,290, filed Oct. 26, 2005.The invention relates to the field of industrial microbiology and the production of alcohols. More specifically, isobutanol is produced via industrial fermentation of a recombinant microorganism.Butanol is an important industrial chemical, useful as a fuel additive, as a feedstock chemical in the plastics industry, and as a foodgrade extractant in the food and flavor industry. Each year 10 to 12 billion pounds of butanol are produced by petrochemical means and the need ...

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Номер записи: 28

02-02-2017 дата публикации

ACID-TOLERANT YEAST CELL, METHOD OF PRODUCING ORGANIC ACID USING THE SAME, AND METHOD OF PRODUCING THE YEAST CELL

Номер: US20170029853A1

Автор: Cho Hwayoung, Cho Kwangmyung, Chu Hunsu, Park Jinhwan, Rhee Hongsoon, Yang Dongsik

Принадлежит:

Provided is an acid-tolerant yeast cell, a method of producing an organic acid by using the yeast cell, and a method of producing the yeast cell resistant to acid. 1. An acid-tolerant genetically engineered yeast cell comprisinga genetic modification that increases activity of an enzyme that catalyzes conversion of phosphatidylinositol (PI) and ceramide to inositol phosphorylceramide (IPC) and diacylglycerol (DG);a genetic modification that increases activity of an enzyme, which catalyzes introduction of a double bond to a fatty acyl site of a fatty acyl-CoA;a genetic modification that decreases activity of an enzyme, which catalyzes formation of triacylglycerol (TG) from diacylglycerol (DG) ora combination of the genetic modifications.2. The yeast cell of claim 1 , wherein the enzyme claim 1 , which catalyzes formation of IPC is an IPC synthase; the enzyme that catalyzes introduction of a double bond to a fatty acyl site of a fatty acyl-CoA is an enzyme that belongs to enzyme code (EC) 1.14.19.1; and the enzyme that catalyzes formation of TG from DG is selected from the group consisting of enzymes that belong to EC 2.3.1.22 and 2.3.1.158.3. The yeast cell of claim 2 , wherein the IPC synthase is AUR1; the enzyme claim 2 , which catalyzes introduction of a double bond to a fatty acyl site of a fatty acyl-CoA is OLE1; and the enzyme claim 2 , which catalyzes formation of triacylglycerol (TG) from diacylglycerol (DG) is DGA1 or LRO1.4. The yeast cell of claim 3 , wherein the AUR1 is a polypeptide having at least 95% of sequence identity with amino acid sequence of SEQ ID NO:1 claim 3 , OLE1 is a polypeptide each having at least 95% of sequence identity with amino acid sequence of SEQ ID NO:3 or SEQ ID NO:5 claim 3 , DGA1 is a polypeptide having at least 95% of sequence identity with amino acid sequence of SEQ ID NO:7 claim 3 , and LRO1 is a polypeptide having at least 95% of sequence identity with amino acid sequence of SEQ ID NO: 9.5. The yeast cell of claim 1 , ...

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Номер записи: 29

04-02-2016 дата публикации

CHLOROGLOEOPSIS SP. HOST CELL FOR PRODUCING ETHANOL AND METHOD FOR PRODUCING ETHANOL USING THE SAME

Номер: US20160032294A1

Автор: Enke Heike, FRIEDRICH Alexandra, PIVEN Irina, ULICZKA Frank

Принадлежит: ALGENOL BIOFUELS INC.

One embodiment of the invention provides a genetically enhanced sp. host cell comprising at least one first recombinant gene encoding a first protein for the production of ethanol under the transcriptional control of a first inducible promoter, having at least 85%, 90% or 95% sequence identity to an endogenous inducible promoter of the sp. host cell. 2ChlorogloeopsisChlorogloeopsis fritschii, ChlorogloeopsisChlorogloeopsis. The genetically enhanced sp. host cell of claim 1 , wherein the host cell is PCC6912sp. PCC9212 claim 1 , or sp. ABICyano3.3ChlorogloeopsisChlorogloeopsis fritschii. The genetically enhanced sp. host cell of claim 2 , wherein the host cell is PCC6912.4Chlorogloeopsis. The genetically enhanced sp. host cell of claim 1 , further comprising at least one second recombinant gene encoding a second protein for the production of ethanol.5Chlorogloeopsis. The genetically enhanced sp. host cell of claim 1 , wherein the first recombinant gene encodes pyruvate decarboxylase.6Chlorogloeopsis. The genetically enhanced sp. host cell of claim 4 , wherein the second recombinant gene encodes alcohol dehydrogenase.7Chlorogloeopsis. The genetically enhanced sp. host cell of claim 1 , wherein the first recombinant gene encodes alcohol dehydrogenase E (AdhE) converting Acetyl-CoA into ethanol.8Chlorogloeopsis. The genetically enhanced sp. host cell of claim 4 , wherein both the first and second recombinant gene are under the transcriptional control of the same first endogenous inducible promoter.9Chlorogloeopsis. The genetically enhanced sp. host cell of claim 4 , wherein the first and second recombinant genes are under the transcriptional control of separate first and second promoters.10Chlorogloeopsis. The genetically enhanced sp. host cell of claim 9 , wherein the second promoter is a constitutive promoter.11Chlorogloeopsis. The genetically enhanced sp. host cell of claim 9 , wherein the second promoter is an inducible promoter.12. (canceled)13. (canceled) ...

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Номер записи: 30

17-02-2022 дата публикации

Compositions and methods for biodegrading alcohol

Номер: US20220047682A1

Автор: Tami Bar, Thomas KOEVARY

Принадлежит: Individual

The present invention provides a pharmaceutical composition containing 10 mg to about 100 g KRED and/or a long-acting alcohol dehydrogenase as an active ingredient and a pharmaceutically acceptable carrier. Moreover, provided herein methods for lowering blood alcohol level, methods for preventing a symptom or a risk arising from alcohol consumption and methods for treating a subject afflicted with alcoholism by the administration of the pharmaceutical composition of the invention.

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Номер записи: 31

30-01-2020 дата публикации

PRODUCTION OF XYLITOL FROM GLUCOSE BY A RECOMBINANT STRAIN

Номер: US20200032224A1

Автор: CHANG YIMING, DEFRETIN SOPHIE, DIEFENBACHER MELANIE, GERARD TANIA, HEYSEN ARNAUD, HONDA MALCA SUMIRE, SCHAEFER ASTRID, Schwab Markus, THOR FRIEDERIKE

Принадлежит: ROQUETTE FRERES

The present invention relates to a recombinant microbial host for the production of xylitol, the recombinant microbial host containing a nucleic acid sequence encoding a NAD-specific D-arabitol 4-oxidoreductase (EC 1.1.1.11) using D-arabitol as substrate and producing D-xylulose as product, and a nucleic acid sequence encoding a NADPH-specific xylitol dehydrogenase using D-xylulose as substrate and producing xylitol as product. 1. A method for producing xylitol , the method comprising: a heterologous nucleic acid sequence encoding a NAD+-specific D-arabitol 4-oxidoreductase (EC 1.1.1.11) using D-arabitol as a substrate and producing D-xylulose as a product; and', 'a heterologous nucleic acid sequence encoding a NADPH-specific xylitol dehydrogenase using D-xylulose as a substrate and producing xylitol as a product; and, 'culturing in a culture medium a recombinant host cell comprisingrecovering the produced xylitol,2. The method according to claim 1 , wherein the culture medium provides the microorganism with a carbon source.3. The method according to claim 2 , wherein the carbon source includes D-glucose.4. The method according to claim 1 , wherein the host cell produces D-arabitol from D-glucose.5. The method according to claim 5 , wherein the host cell produces D-arabitol from D-glucose under a high osmotic pressure medium.6. The method according to claim 1 , wherein the host cell does not consume D-arabitol as a sole carbon source.7. The method according to claim 1 , wherein the host cell is selected from bacteria claim 1 , fungi claim 1 , and yeast.8. The method according to claim 1 , wherein the host cell is an osmophilic or osmotolerant yeast.9Pichia ohmeri.. The method according to claim 1 , wherein the host cell is10E. coliRaisionia solanacearum.. The method according to claim 1 , wherein the NAD+-specific D-arabitol 4-oxidoreductase (EC 1.1.1.11) is from and/or11. The method according to claim 1 , wherein the NAD+-specific D-arabitol 4-oxidoreductase (EC 1. ...

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Номер записи: 32

04-02-2021 дата публикации

MATERIALS AND METHODS FOR THE BIOSYNTHESIS OF SEVEN CARBON CHEMICALS IN THE PRESENCE OF METHANOL OXIDATION

Номер: US20210032632A1

Автор: CHOKKATHUKALAM Achuthanunni, MOMO Remi Ako Mbianyor, VAN ECK CONRADIE Alex

Принадлежит:

This disclosure describes methods for regulating the biosynthesis of pimelic acid, 7-aminoheptanoate, 7-hydroxyheptanoate, heptamethylenediamine, 7-aminoheptanol, or 1,7-heptanediol by channeling increased flux through the biosynthesis pathway to obtain an intermediate required for growth of the host microorganism. 1. A method for regulating biosynthesis of a product in a host cell , wherein the product comprises pimelic acid , 7-aminoheptanoate , 7-hydroxyheptanoate , heptamethylenediamine , 7-aminoheptanol , or 1 ,7-heptanediol , or salts thereof , using a pathway having a pimeloyl-acyl carrier protein (pimeloyl-ACP) intermediate , the method comprising converting methanol to formate via at least one spontaneous enzymatic reaction , wherein the formate is used in the conversion of tetrahydrofolate to N-formyl-tetrahydrofolate , wherein:the methanol is produced during pimeloyl-ACP methyl ester esterase (BioH) enzyme activity in which BioH removes a methyl group from pimeloyl-ACP methyl ester during conversion of pimeloyl-ACP methyl ester to pimeloyl ACP, wherein the BioH activity is increased compared to an unmodified host cell; andthe method comprises downregulating (a) bifunctional protein (FolD) activity by attenuating folD and/or (b) formate acetyltransferase 1 (PflB) activity and formate acetyltransferase-like enzyme (TdcE) activity: andthe method comprises inserting into the host cell (i) a gene encoding an S-formylglutathione hydrolase (frmB), (ii) a gene encoding a formate-tetrahydrofolate ligase (fhs), (iii) a gene encoding an alcohol dehydrogenase (adh), and (iv) a gene encoding an S-(hydroxymethyl) glutathione dehydrogenase (frmA),wherein the S-formylglutathione hydrolase has at least 90% sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NOs: 27-30, the formate-tetrahydrofolate ligase has at least 90% sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NOs: 18-22, the alcohol ...

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Номер записи: 33

11-02-2016 дата публикации

Engineered co2 fixing microorganisms producing carbon-based products of interest

Номер: US20160040191A1

Автор: Brian D. Green, Christian Perry Ridley, Dan Eric Robertson, David Arthur Berry, Frank Anthony Skraly, Martha Sholl, Nikos Basil Reppas, Noubar Boghos Afeyan, Sriram Kosuri

Принадлежит: JOULE BIOTECHNOLOGIES, Joule Unlimited Technologies Inc

The present disclosure identifies pathways and mechanisms to confer production of carbon-based products of interest such as ethanol, ethylene, chemicals, polymers, n-alkanes, isoprenoids, pharmaceutical products or intermediates thereof in photoautotrophic organisms such that these organisms efficiently convert carbon dioxide and light into carbon-based products of interest, and in particular the use of such organisms for the commercial production of ethanol, ethylene, chemicals, polymers, n-alkanes, isoprenoids, pharmaceutical products or intermediates thereof.

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Номер записи: 34

11-02-2016 дата публикации

ENZYMATIC CONVERSION OF BOTH ENANTIOMERS OF 1,2-PROPANEDIOL TO PROPIONALDEHYDE

Номер: US20160040196A1

Автор: Lanzilotta William

Принадлежит:

Engineered organisms, cell-free enzyme mixtures, and methods are provided for converting both enantiomers of 1,2-propanediol to propionaldehyde. Engineered organisms are provided that convert both enantiomers of 1,2-propanediol to propionaldehyde but do not convert glycerol to 3-hydroxypropionaldehyde and/or do not convert propanal to propanol. The engineered organisms and cell-free enzyme mixtures can contain a diol dehydratase enzyme similar in sequence identity to diol dehydratase. The engineered organisms and cell-free enzyme mixtures can contain a diol dehydratase activating enzyme similar in sequence identity to diol dehydratase activating enzyme. Methods of converting both enantiomers of 1-2-propanediol to propanol can include culturing a microorganism provided herein under conditions and for a period of time sufficient to convert the 1,2-propanediol to propanol. The conditions can include a substantially anaerobic culture medium. 1. A non-naturally occurring microorganism comprising an exogenous diol dehydratase enzyme.2Roseburia inulinivorans. The non-naturally occurring microorganism of claim 1 , wherein the enzyme is at least 80% identical in protein sequence to diol dehydratase (RiDD).3Roseburia inulinivorans. The non-naturally occurring microorganism of claim 1 , wherein the enzyme is diol dehydratase (RiDD).4. The non-naturally occurring microorganism of that does not convert glycerol to 3-hydroxypropionaldehyde.5. The non-naturally occurring microorganism of that does not convert propanal to propanol.6. The non-naturally occurring microorganism of claim 1 , comprising an exogenous nucleic acid that encodes for the enzyme.7. The non-naturally occurring microorganism of claim 1 , wherein the microorganism is in a substantially anaerobic culture medium.8. The non-naturally occurring microorganism of claim 1 , further comprising an exogenous diol dehydratase activating enzyme in an amount sufficient to activate the diol dehydratase enzyme.9. The non- ...

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Номер записи: 35

09-02-2017 дата публикации

SACCHAROMYCES CEREVISIAE STRAINS

Номер: US20170037361A1

Автор: Bonander Nicklas

Принадлежит:

The present invention relates to a method of preparing a strain of sugar fermenting with capability to ferment xylose, wherein said method comprises different procedural steps. The method comprises mating a first sporulated strain with a second haploid strain. Thereafter, screening for mated cells is performed, growing such mated cells, and verifying that mated cells exhibit basic morphology by microscopic inspection. Thereafter, creation of a mixture of the mated cells is performed, subjecting the mixture to continuous chemostat lignocellulose cultivation and obtaining the sugar fermenting cells with capability to ferment xylose is performed. The invention also comprises strains obtained by said method. 1Saccharomyces cerevisiae. A strain of comprising at least one native XKS1 gene in its genome encoding xylulokinase , at least one native XDH1 gene in its genome encoding xylitol dehydrogenase , and at least one modGre3 gene in its genome , said modGre3 gene encoding an amino acid sequence of SEQ ID NO 1 having xylose reductase activity or encoding a fragment of said amino acid sequence having xylose reductase activity , wherein said strain is obtained by the following steps:{'i': 'Saccharomyces cerevisiae', 'a) sporulating a first strain of for providing at least 20 tetrads of said strain,'}{'i': Scheffersomyces stipitis', 'Saccharomyces cerevisiae', 'Saccharomyces cerevisiae,, 'b) introducing DNA, encoding for xylose reductase and xylitol dehydrogenase obtained from and xylulokinase obtained from , into a second strain of'}{'i': Saccharomyces cerevisiae', 'Saccharomyces cerevisiae', 'Saccharomyces cerevisiae, 'c) mating the first sporulated strain with the second strain evolved on xylose and in a haploid state by mixing cells of said haploid strain with each tetrad obtained in step a) to provide mated cells on an YPD agar plate,'}d) screening for mated cells on xylose and geneticin agar plates,e) growing mated cells from step d) in minimal defined xylose liquid ...

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Номер записи: 36

09-02-2017 дата публикации

SYNTHETIC METHANOTROPHIC AND METHYLOTROPHIC MICROORGANISMS

Номер: US20170037438A1

Автор: CLARKE Elizabeth, GREENFIELD Derek, HELMAN Noah

Принадлежит: INDUSTRIAL MICORBES, INC.

Provided herein are non-naturally occurring microbial organisms comprising a methane-oxidizing metabolic pathway. The invention additionally comprises non-naturally occurring microbial organisms comprising pathways for the production of chemicals. The invention additionally provides methods for using said organisms for the production of chemicals. 1. A synthetic microorganism , wherein said synthetic microorganism comprises a natural methanol-consuming microorganism and one or more genetic modifications that improve the production of a chemical.2. (canceled)3. A synthetic microorganism comprising a natural non-methanol-consuming microorganism and one or more genetic modifications that allow said synthetic microorganism to oxidize methanol.4Escherichia coli, Bacillus subtilis, Pseudomonas putida, Saccharomyces cerevisiae, Corynebacterium glutamicum Klebsiella oxytoca, Anaerobiospirillum succiniciproducens, Actinobacillus succinogenes, Mannheimia succiniciproducens, Rhizobium etli, Gluconobacter oxydans, Zymomonas mobilis, Lactococcus lactis, Lactobacillus plantarum, Streptomyces coelicolor, Clostridium acetobutylicum, Pseudomonas fluorescens, Schizosaccharomyces pombe, Kluyveromyces lactis, Kluyveromyces marxianus, Aspergillus terreusAspergillus niger.. The synthetic microorganism of claim 3 , wherein the natural non-methanol-consuming microorganism is selected from the group consisting of claim 3 , and5Corynebacterium glutamicumEscherichia coli.. The synthetic microorganism of claim 4 , wherein the natural non-methanol-consuming microorganism is not or6. The synthetic microorganism of claim 3 , further comprising exogenous polynucleotides claim 3 , wherein said exogenous polynucleotides encode enzymes selected from the group consisting of methanol dehydrogenase (EC 1.1.1.224 or 1.1.99.37 or 1.1.2.7) claim 3 , alcohol dehydrogenase (EC 1.1.1.1) claim 3 , 3-hexulose-6-phosphate synthase (EC 4.1.2.43) and 6-phospho-3-hexuloisomerase (EC 5.3.1.27).7. The synthetic ...

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Номер записи: 37

08-02-2018 дата публикации

Electrochemical Bioreactor Module and Engineered Metabolic Pathways for 1-Butanol Production with High Carbon Efficiency

Номер: US20180037914A1

Автор: Armiger William B., Dodds David R., Koffas Mattheos

Принадлежит: Biocheminsights, Inc.

A combination of an electrochemical device for delivering reducing equivalents to a cell, and engineered metabolic pathways within the cell capable of utilizing the electrochemically provided reducing equivalents is disclosed. Such a combination allows the production of commodity chemicals by fermentation to proceed with increased carbon efficiency. 1. A system for 1-butanol production , comprising:an electrochemical bioreactor module for providing reducing equivalents;a first engineered pathway for producing 1-butanol from acetyl-CoA; anda second engineered pathway for recovering carbon as formate from pyruvate, and converting the recovered formate to fructose-6-phosphate;wherein the reducing equivalents are provided to one or more redox enzymes in the first and/or second engineered pathways; and wherein optionally the first and second engineered pathways are present in an engineered cell.2. The system of wherein the first engineered pathway comprises acetyl-CoA acetyltransferase (AtoB claim 1 , EC 2.3.1.9) claim 1 , 3-hydroxybutyryl-CoA dehydrogenase (Hbd claim 1 , EC 1.1.1.157) claim 1 , 3-hydroxybutyryl-CoA dehydratase (Crt claim 1 , EC 4.2.1.5) claim 1 , trans-enoyl-CoA reductase (Ter claim 1 , EC 1.3.1.38) and aldehyde/alcohol dehydrogenase (AdhE2 claim 1 , EC 1.2.157/EC 1.1.1.1).3. The system of or wherein the second engineered pathway comprises pyruvate:formate lyase (Pfl claim 1 , EC 2.3.1.54) claim 1 , formaldehyde dehydrogenase (Fld claim 1 , EC 1.2.1.46) claim 1 , hexulose-6-phosphate synthase (HPS claim 1 , EC 4.1.2.43) claim 1 , and 6-phospho-3-hexuloisomerase (HPI claim 1 , EC 5.3.1.27).4. The system of or wherein in the engineered cell claim 1 , the endogenous pyruvate dehydrogenase (Pdh claim 1 , EC 1.2.4.1) has been disabled claim 1 , deleted or otherwise rendered non-functional.5. The system of or wherein in the engineered cell claim 1 , the endogenous fumarate reductase (FrdBC claim 1 , EC 1.3.1.6) claim 1 , lactate dehydrogenase (Ldh claim 1 , ...

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Номер записи: 38

12-02-2015 дата публикации

Replacement Therapy for Dental Caries

Номер: US20150044146A1

Автор: Hillman Jeffrey D., SMITH JAMES LEIF, Wilson-Stanford Shawanda R.

Принадлежит:

The invention provides recombinant strains that can be used to improve oral health. An embodiment of the invention provides a method of reducing the incidence or severity of dental caries in a dental caries-susceptible host comprising administering orally to the host an isolated recombinant strain of the invention in an amount effective for replacement of dental caries-causing host strains in the oral cavity of the host. The isolated recombinant strain 10 can be contained in a mouthwash, toothpaste, chewing gum, floss, chewable tablet, food, or beverage. 2Streptococcus mutans. The isolated recombinant strain of claim 1 , wherein the mutacin comprising Formula I further comprises a Trp4insAla mutation or a ΔTrp4 mutation.3Streptococcus mutans. The isolated recombinant strain of claim 1 , wherein the following amino acid substitutions are present: Abu8Ala claim 1 , or Dhb14Ala claim 1 , or both Abu8Ala and Dhb14Ala in the lantibiotic comprising Formula I.4Streptococcus mutansS. mutans. The isolated recombinant strain of claim 1 , further comprising a mutation in a polynucleotide involved in ComE claim 1 , ComC or both ComE and Com C synthesis such that expression of ComE claim 1 , ComC claim 1 , or both ComE and ComC is diminished by about 80% or more as compared to a wild-type strain.5Streptococcus mutansStreptococcus mutans. The isolated recombinant strain of claim 1 , further comprising a mutation in a polynucleotide involved in D-amino acid synthesis such that expression of the D-amino acid is diminished by about 80% or more as compared to a wild-type strain.6Streptococcus mutans. The isolated recombinant strain of claim 5 , wherein the polynucleotide is dal or a promoter for dal.7Streptococcus mutansZymomonas mobilisStreptococcus mutans. The isolated recombinant strain of claim 1 , wherein the recombinant alcohol dehydrogenase polynucleotide is a alcohol dehydrogenase polynucleotide or a alcohol dehydrogenase polynucleotide.8Streptococcus mutansStreptococcus ...

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Номер записи: 39

18-02-2021 дата публикации

COMPOSITIONS AND METHODS FOR INCREASING ETHANOL PRODUCTION BY YEAST USING GCY1 AND DAK1

Номер: US20210047660A1

Автор: Teunissen Paula Johanna Maria, Zhu Quin Qun

Принадлежит:

Described are compositions and methods relating to yeast expressing glycerol dehydrogenase and dihydroxyacetone kinase polypeptides in combination with an exogenous phosphoketolase pathway, as well as to bifunctional glycerol dehydrogenase-dihydroxyacetone kinase fusion polypeptides, and their various and combined uses in starch hydrolysis processes for alcohol production. 1. A fusion polypeptide comprising a first amino acid sequence having glycerol dehydrogenase activity fused to a second amino acid sequence having dihydroxyacetone kinase activity , wherein the fusion polypeptide , when expressed in a yeast cell , is capable of converting glycerol to dihydroxyacetone phosphate.2. The fusion polypeptide of claim 1 , wherein the first amino acid sequence and second amino acid sequence are fused via a linker peptide.3. The fusion polypeptide of or claim 1 , wherein the first amino acid sequence is present at the N-terminus of the fusion polypeptide and second amino acid sequence is present at the C-terminus of the fusion polypeptide.4. The fusion polypeptide of or claim 1 , wherein the second amino acid sequence is present at the N-terminus of the fusion polypeptide and first amino acid sequence is present at the C-terminus of the fusion polypeptide.5Saccharomyces. The fusion polypeptide of any of the preceding claims claim 1 , wherein the first amino acid sequence is the glycerol dehydrogenase from a sp. or a structural or functional homolog claim 1 , thereof.6Saccharomyces. The fusion polypeptide of any of the preceding claims claim 1 , wherein the second amino acid sequence is the dihydroxyacetone kinase from a sp. or a structural or functional homolog claim 1 , thereof.7. A DNA sequence encoding the fusion polypeptide of any of the preceding claims claim 1 , optionally capable of overexpressing the fusion polypeptide compared to the individual expression levels of either or both GCY1 or DAK1 claim 1 , based on mRNA levels claim 1 , compared to a parental yeast ...

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Номер записи: 40

16-02-2017 дата публикации

HIGH YIELD ROUTE FOR THE PRODUCTION OF COMPOUNDS FROM RENEWABLE SOURCES

Номер: US20170044551A1

Автор: Chokhawala Harshal

Принадлежит:

Provided herein are methods, compositions, and non-naturally occurring microbial organism for preparing compounds such as 1-butanol, butyric acid, succinic acid, 1,4-butanediol, 1-pentanol, pentanoic acid, glutaric acid, 1,5-pentanediol, 1-hexanol, hexanoic acid, adipic acid, 1,6-hexanediol, 6-hydroxy hexanoic acid, ε-Caprolactone, 6-amino-hexanoic acid, ε-Caprolactam, hexamethylenediamine, linear fatty acids and linear fatty alcohols that are between 7-25 carbons long, linear alkanes and linear α-alkenes that are between 6-24 carbons long, sebacic acid and dodecanedioic acid comprising: a) converting a Caldehyde and pyruvate to a Cβ-hydroxyketone intermediate through an aldol addition; and b) converting the Cβ-hydroxyketone intermediate to the compounds through enzymatic steps, or a combination of enzymatic and chemical steps. 1. A non-naturally occurring microbial organism comprising at least one exogenous nucleic acid encoding a 1 ,6-hexanediol pathway enzyme.2. The microbial organism of further comprising at least one enzyme selected from 2A wherein 2A is a 4-hydroxy-2-oxo-adipate aldolase claim 1 , or a 4 claim 1 ,6-dihydroxy-2-oxo-hexanoate aldolase.327-. (canceled)28. A non-naturally occurring microbial organism claim 1 , comprising at least one exogenous nucleic acid encoding a 1 claim 1 ,6-hexanediol pathway enzyme selected from 2A and one or more of 2B claim 1 , 3B1 claim 1 , 3B2 claim 1 , wherein 2A is a 4-hydroxy-2-oxo-adipate aldolase or a 4 claim 1 ,6-dihydroxy-2-oxo-hexanoate aldolase claim 1 , 2B is a 4-hydroxy-2-oxo-adipate dehydratase or a 4 claim 1 ,6-dihydroxy-2-oxo-hexanoate 4-dehydratase claim 1 , 3B1 is a 4-hydroxy-2-oxo-adipate 2-reductase or a 4 claim 1 ,6-dihydroxy-2-oxo-hexanoate 2-reductase claim 1 , and 3B2 is a 4-hydroxy-2-oxo-adipate 4-dehydrogenase or a 4 claim 1 ,6-dihydroxy-2-oxo-hexanoate 4-dehydrogenase.29. The organism of claim 28 , further comprising a 1 claim 28 ,6-hexanediol pathway enzyme selected from one or more of 2C claim ...

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Номер записи: 41

16-02-2017 дата публикации

Polypeptides with Ketol-Acid Reductoisomerase Activity

Номер: US20170044578A1

Автор: LI YOUGEN, MCELVAIN JESSICA, ROTHMAN STEVEN CARY

Принадлежит:

Polypeptides having ketol-acid reductoisomerase activity are provided. Also disclosed are recombinant host cells comprising isobutanol biosynthetic pathways employing such polypeptides. Methods for producing isobutanol employing host cells comprising the polypeptides having ketol-acid reductoisomerase activity are also disclosed. 1. A recombinant host cell comprising an isobutanol biosynthetic pathway anda. a heterologous polypeptide with ketol-acid reductoisomerase activity having at least about 85%, at least about 90% identity, at least about 95%, or at least about 98% identity to one of the following: K9JM2 (SEQ ID NO: 193), K9JM3 (SEQ ID NO: 194), K9JM4 (SEQ ID NO: 195), K9JM5 (SEQ ID NO: 196), K9JM6 (SEQ ID NO: 197), K9JM7 (SEQ ID NO: 198), K9JM8 (SEQ ID NO: 199), K9JM9 (SEQ ID NO: 200), K9JM10 (SEQ ID NO: 201), K9JM11 (SEQ ID NO: 202), K9JM12 (SEQ ID NO: 203), K9JM13 (SEQ ID NO: 204), K9JM14 (SEQ ID NO: 205), K9JM15 (SEQ ID NO: 206), K9JM16 (SEQ ID NO: 207), K9JM17 (SEQ ID NO: 208), K9JM18 (SEQ ID NO: 209), K9JM19 (SEQ ID NO: 210), K9JM20 (SEQ ID NO: 211), K9JM21 (SEQ ID NO: 212), K9JM22 (SEQ ID NO: 213), K9JM23 (SEQ ID NO: 214), K9JM24 (SEQ ID NO: 215), K9JM25 (SEQ ID NO: 216), K9JM26 (SEQ ID NO: 217), K9JM27 (SEQ ID NO: 218), K9JM28 (SEQ ID NO: 219), K9JM29 (SEQ ID NO: 220), K9JM30 (SEQ ID NO: 221), K9JM31 (SEQ ID NO: 222), JM32 (SEQ ID NO: 223), JM33 (SEQ ID NO: 224), JM34 (SEQ ID NO: 225), JM35 (SEQ ID NO: 226), JM36 (SEQ ID NO: 227), JM37 (SEQ ID NO: 228), JM38 (SEQ ID NO: 229), JM39 (SEQ ID NO: 230), JM40 (SEQ ID NO: 231), JM42 (SEQ ID NO: 232), JM43 (SEQ ID NO: 233), JM44 (SEQ ID NO: 234), K9SB2 (SEQ ID NO: 235), K9_DAVID_SH (SEQ ID NO: 236), K9ALL3 (SEQ ID NO: 237), K9_URSALA (K9SB2+A56V) (SEQ ID NO: 239), JM41 (SEQ ID NO: 240), K9ALL148 (SEQ ID NO: 241), K9JM148 (SEQ ID NO: 242), K9ALL156 (SEQ ID NO: 243), K9JM156 (SEQ ID NO: 244), K9ALL191 (SEQ ID NO: 245), K9JM191 (SEQ ID NO: 246), K9ALL254 (SEQ ID NO: 247), K9ALL278 (SEQ ID NO: 248), K9ALL37 (SEQ ...

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Номер записи: 42

03-03-2022 дата публикации

ANAEROBIC FERMENTATIVE PRODUCTION OF FURANDICARBOXYLIC ACID

Номер: US20220064683A1

Автор: GOUVEA Iuri Estrada, Marchesin Mariana Trovo, ROMÃO DUMARESQ Aline Silva, SIMOES Marcos Rogerio

Принадлежит:

The present disclosure provides recombinant microorganisms and methods for the anaerobic production of 2,4-furandicarboxylic acid from one or more carbon sources. The microorganisms and methods provide redox-balanced and ATP positive pathways for co-producing 2,4-furandicarboxylic acid with ethanol and for co-producing 2,4-furandicarboxylic acid with ethanol and 1-propanol. The method provides recombinant microorganisms that express endogenous and/or exogenous nucleic acid molecules encoding polypeptides that catalyze the conversion of a carbon source into 2,4-furandicarboxylic acid and that coupled the 2,4-furandicarboxylic acid pathway with an additional metabolic pathway.

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Номер записи: 43

14-02-2019 дата публикации

A BACTERIAL CELL FACTORY FOR EFFICIENT PRODUCTION OF ETHANOL FROM WHEY

Номер: US20190048368A1

Автор: Dantoft Shruti Harnal, Jensen Peter Ruhdal, LIU Jianming, Solem Christian

Принадлежит:

The invention relates to a method for homo-ethanol production from lactose using a genetically modified lactic acid bacterium of the invention, where the cells are provided with a substrate comprising dairy waste supplemented with an amino nitrogen source (such as acid hydrolysed corn steep liquor). The invention further relates to genetically modified lactic acid bacterium and its use for homo-ethanol production from lactose in dairy waste. The lactic acid bacterium comprises both genes (lacABCD, LacEF, lacG) encoding enzymes catalysing the lactose catabolism pathway; and transgenes (pdc and adhB) encoding enzymes catalysing the conversion of pyruvate to ethanol. Additionally a number of genes (ldh, pta and adhE) are deleted in order to maximise homo-ethanol production as compared to production of lactate, acetoin and acetate production. 1. A method for ethanol production using a genetically engineered lactic acid bacterium comprising the steps of:a. introducing a genetically modified lactic acid bacterium into an aqueous culture medium;b. incubating the culture of (a);c. recovering ethanol produced by said culture during step (b), and optionally wherein the aqueous culture medium comprises:', 'I. whey permeate or residual whey permeate, and', wherein the genetically engineered lactic acid bacterium comprises transgenes encoding:', 'i. a polypeptide having pyruvate decarboxylase (PDC) activity (EC 4.1.1.1); and', 'wherein the genome of said lactic acid bacterium comprises genes encoding polypeptides having:', 'ii. a polypeptide having alcohol dehydrogenase B activity (EC 1.1.1.1); and'}, 'iii. lactose-specific phosphotransferase system (PTS) activity (EC 2.7.1.69)', 'iv. phospho-β-D-galactosidase activity (EC 3.2.1.85)', 'v. galactose-6-phosphate isomerase activity (EC 5.3.1.26),', 'vi. D-tagatose-6-phosphate kinase activity (EC 2.7.1.114), and', 'wherein the genome of said lactic acid bacterium is deleted for genes or lacks functional genes or genes encoding ...

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Номер записи: 44

25-02-2016 дата публикации

METHOD FOR THE ISOMERISATION OF GLUCOSE

Номер: US20160053289A1

Автор: Ertl Ortwin

Принадлежит:

Disclosed is a method for the isomerisation of glucose by reduction to sorbitol and subsequent oxidation to fructose, in which the redox cofactors NAD/NADH and NADP/NADPH are regenerated in a one-pot-reaction, wherein one of the two redox cofactors is obtained in the reduced form thereof and the other redox cofactor in the oxidised form thereof as a result of at least two additional enzymatically catalysed redox reactions (product forming reactions) taking place in the same reaction batch, wherein a) in the regeneration reaction, which transfers the reduced cofactor back to its originally oxidised form, oxygen or a compound of the general formula RC(O)COOH is reduced, and b) in the regeneration reaction, which transfers the oxidised cofactor back to its originally reduced form, a compound of the general formula RCH(OH)Ris oxidised, wherein R, Rand Rhave different meanings in the compounds, characterised in that a mixture of glucose and fructose is used as a starting material. Furthermore, the use of fructose thus produced in a method for producing furan derivatives is disclosed. 2. The method according to claim 1 , wherein in a) a compound of the general formula I claim 1 , wherein Ris a substituted or unsubstituted (C)alkyl group claim 1 , is reduced claim 1 , and in b) a compound of the general formula II claim 1 , wherein Rand Rare independently selected from the group consisting of H claim 1 , (C)alkyl claim 1 , wherein alkyl is straight or branched claim 1 , (C)alkenyl claim 1 , wherein alkenyl is straight or branched and optionally contains up to three double bonds claim 1 , cycloalkyl claim 1 , aryl claim 1 , (C)carboxyalkyl claim 1 , if compound I is a pyruvate claim 1 , optionally also carboxyl claim 1 , is oxidised.3. The method according to claim 1 , wherein in b) a compound of formula II claim 1 , wherein Rand Rare independently selected from the group consisting of H claim 1 , (C-C)alkyl claim 1 , wherein alkyl is straight or branched claim 1 , (C) ...

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Номер записи: 45

25-02-2021 дата публикации

METHOD TO PRODUCE ENANTIOMERS OF UNDECAVERTOL

Номер: US20210054417A1

Автор: BAUMGARTNER Corinne, BIERMANN Marc, ELLWOOD Simon

Принадлежит:

A method for increasing the proportion of an enantiomer of undecavertol in an enantiomeric mixture of undecavertol, a method for stereoselectively synthesising undecavertol, and the products thereof. 1. A method of increasing the proportion of an enantiomer of undecavertol in an enantiomeric mixture of undecavertol , the method comprising contacting the enantiomeric mixture of undecavertol with an alcohol dehydrogenase (ADH) and an ADH-cofactor.2. A method of stereoselectively synthesising undecavertol , the method comprising contacting undecavertone with an alcohol dehydrogenase (ADH) and an ADH-cofactor.3. The method of claim 1 , wherein the ADH-cofactor is selected from NADPH claim 1 , NADH claim 1 , quinoid cofactors claim 1 , zinc or a combination of one or more thereof.4. The method of claim 1 , wherein the method further comprises contacting the ADH-cofactor with an ADH-cofactor regeneration system.5. The method of claim 4 , wherein the ADH-cofactor regeneration system is a substrate-coupled regeneration system.6. The method of claim 4 , wherein the ADH-cofactor regeneration system is an enzyme-coupled regeneration system.7. The method of claim 1 , wherein the ratio of (R)-enantiomers to (S)-enantiomers in the enantiomeric mixture of undecavertol prior to contacting with the ADH and ADH-cofactor ranges from about 45:55 to about 55:45.8. The method of claim 1 , wherein the enantiomeric mixture of undecavertol prior to contacting with the ADH and ADH-cofactor is a racemic mixture.9. The method of claim 1 , wherein the method results in a product having an enantiomeric excess equal to or greater than about 94%.10. The method of claim 1 , wherein the method results in a product having an enantiomeric excess of (R)-enantiomer equal to or greater than about 94%.11. The method of claim 1 , wherein the contacting step occurs for a period of time ranging from about 30 minutes to about 3 days.12. The method of claim 1 , wherein the contacting step occurs at a ...

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Номер записи: 46

04-03-2021 дата публикации

Recombinant host cells and methods for the production of glyceric acid and downstream products

Номер: US20210061748A1

Автор: Jeffrey A. Dietrich, Johan Van Walsem, Mario Ouellet

Принадлежит: Lygos, Inc.

Methods and materials related to producing glyceric acid and downstream products are disclosed. Specifically, isolated nucleic acids, polypeptides, host cells, methods and materials for producing glycolic acid by direct fermentation from sugars are disclosed.

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Номер записи: 47

04-03-2021 дата публикации

VARIANT MICROORGANISM HAVING ABILITY TO PRODUCE 1,3-PROPANEDIOL, AND METHOD FOR PREPARING 1,3-PDO BY USING SAME

Номер: US20210062232A1

Автор: CHO Changhee, CHO Jae Sung, CHOI Jae Won, HAN TAEHEE, KIM Euiduk, KIM Je Woong, KO Yoo Sung, LEE JUN KYU, LEE Sang Yup, PRABOWO Cindy Pricilia Surya

Принадлежит:

The present disclosure relates to a mutant microorganism in which a glycerol catabolic pathway and a 1,3-PDO biosynthetic pathway are introduced into a microorganism incapable of using glycerol as a carbon source, and a method of producing 1,3-PDO using the same. According to the present disclosure, it is possible to produce 1,3-PDO while growing a mutant microorganism having 1,3-PDO production ability by using the inexpensive raw material glycerol as a single carbon source. Thus, the present disclosure is useful for the economical production of 1,3-PDO. 1. A mutant microorganism in which a glycerol facilitator-encoding gene , a glycerol kinase-encoding gene and a glycerol dehydrogenase-encoding gene are introduced into a microorganism incapable of using glycerol as a single carbon source and which is capable of growing on glycerol as a single carbon source.2. The mutant microorganism of claim 1 , wherein the glycerol facilitator-encoding gene claim 1 , the glycerol kinase-encoding gene and the glycerol dehydrogenase-encoding gene are glpF claim 1 , glpK and glpD claim 1 , respectively.3Corynebacterium glutamicum spp., Lactobacillus panis, Clostridium acetobutylicum, Clostridium beijerinckii, Mycobacterium tuberculosisRhodobacter capsulatus.. The mutant microorganism of claim 1 , wherein the microorganism incapable of using glycerol as a single carbon source is a microorganism selected from the group consisting of claim 1 , and4. The mutant microorganism of claim 1 , wherein the genes are overexpressed by a strong promoter selected from the group consisting of tac claim 1 , trc claim 1 , H36 and tuf.5. A mutant microorganism in which a glycerol facilitator-encoding gene claim 1 , a glycerol kinase-encoding gene claim 1 , a glycerol dehydrogenase-encoding gene claim 1 , a glycerol dehydratase-encoding gene claim 1 , a glycerol reactivase-encoding gene and a 1 claim 1 ,3-propanediol oxidoreductase-encoding gene are introduced into a microorganism incapable of using ...

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Номер записи: 48

28-02-2019 дата публикации

Ketoreductases

Номер: US20190062714A1

Автор: Andreas Petri, Andreas Vogel, Claudia Feller, Daniel Schwarze, Hedda Merkens, Kamila Rzeznicka, Marc Struhalla, Ramona Schmiedel, Rico Czaja, Sabrina Koepke, Thomas Greiner-Stoeffele

Принадлежит: C Lecta GmbH

The invention relates to ketoreductases and the use thereof. The ketoreductases of the invention are particularly useful for enzymatically catalyzing the reduction of ketones to chiral secondary alcohols.

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Номер записи: 49

10-03-2016 дата публикации

Methods and Compositions for Producing Solvents

Номер: US20160068802A1

Автор: Blaschek Hans P., SHI Zhen, Stoddard Steven F.

Принадлежит:

Described herein are methods, compositions and synthetic biology approaches for solvent production, including but not limited to butanol production. Described herein are recombinant bacteria and yeast strains which may be used in production of a solvent, including but not limited to butanol, from lignocellulosic and other plant-based feedstocks. Described herein are methods of producing solvents, including but not limited to butanol, using bacteria and yeast strains. Described herein are methods of producing organisms that display highly efficient butanol production. 161.-. (canceled)62Clostridium beijerinckii. A recombinant solventogenic organism , wherein the organism is transformed with a nucleotide molecule comprising at least one Adh (alcohol dehydrogenase) polynucleotide , wherein the Adh polynucleotide comprises at least 95% identity to the sequence selected from the group consisting of (i) SEQ ID NO: 14 , (ii) the sequence that encodes a protein comprising the amino acid sequence of SEQ ID NO: 15 , and (iii) the complement of any thereof , to form the recombinant solventogenic organism , whereby the recombinant solventogenic organism is capable of (i) more efficient solvent production , (ii) faster solvent production , and/or (iii) increased solvent production relative to an organism that is not transformed with the nucleotide molecule.63. The recombinant solventogenic organism of claim 62 , wherein the organism is further transformed with a polynucleotide comprising at least 95% identity to the sequence of SEQ ID NO:2 or 3 or complements thereof.64. The recombinant solventogenic organism of claim 62 , wherein the organism is further transformed with a polynucleotide comprising at least 95% identity to the sequence of SEQ ID NO:4 claim 62 , 5 claim 62 , 6 claim 62 , or complements thereof.65Clostridium beijerinckii. The recombinant solventogenic organism of claim 62 , wherein the Adh polynucleotide is controlled by an inducible or a constitutive promoter.66. ...

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Номер записи: 50

10-03-2016 дата публикации

Microorganisms and methods for producing pyruvate, ethanol, and other compounds

Номер: US20160068871A1

Автор: Jennifer L. Reed, Xiaolin Zhang

Принадлежит: WISCONSIN ALUMNI RESEARCH FOUNDATION

Microorganisms comprising modifications for producing pyruvate, ethanol, and other compounds. The microorganisms comprise modifications that reduce or ablate activity of one or more of pyruvate dehydrogenase, 2-oxoglutarate dehydrogenase, phosphate acetyltransferase, acetate kinase, pyruvate oxidase, lactate dehydrogenase, cytochrome terminal oxidase, succinate dehydrogenase, 6-phosphogluconate dehydrogenase, glutamate dehydrogenase, pyruvate formate lyase, pyruvate formate lyase activating enzyme, and isocitrate lyase. The microorganisms optionally comprise modifications that enhance expression or activity of pyruvate decarboxylase and alcohol dehydrogenase. The microorganisms are optionally evolved in defined media to enhance specific production of one or more compounds. Methods of producing compounds with the microorganisms are provided.

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Номер записи: 51

11-03-2021 дата публикации

FERMENTATION PROCESS FOR IMPROVED GLYCEROL AND ACETIC ACID CONVERSION

Номер: US20210071205A1

Автор: DE BRUIJN Hans Marinus Charles Johannes, de Laat Wilhelmus Theodorus Antonius Maria, Klaassen Paul

Принадлежит:

The invention relates to a process for producing a fermentation product that comprises fermentation of a carbon source in a reactor with a cell, capable of converting sugar, glycerol and acetic acid, wherein the carbon source comprises sugar and acetic acid, comprising the following steps: 1. Process for producing a fermentation product that comprises fermentation of a carbon source in a reactor with a cell , capable of converting sugar , glycerol and acetic acid , wherein the carbon source comprises sugar and acetic acid , comprising:a) Inoculating a optionally diluted carbon source with the cell;b) optionally fermenting the reactor in batch mode;c) adding carbon source comprising glycerol and optionally sugar gradually to the reactor;d) after sufficient fermentation time, isolation of fermentation product from the reactor,e) optionally keeping the remaining fraction after isolation of d) as spent broth; andf) optionally using the spent broth in a) to dilute the carbon source.2. Process according to claim 1 , wherein the carbon source comprises lignocellulosic hydrolysate.3. Process according to claim 2 , wherein the amount of glycerol added is such that the molar concentration of glycerol in the reactor is about twice the molar concentration or 1.8 to 2.2 times the molar concentration of acetic acid in the reactor.4. Process according to claim 2 , wherein the added glycerol originates from a starch or sugar based ethanol product plant or a biodiesel plant.5. Process according to claim 1 , wherein the addition of glycerol is commenced when the glucose concentration in reactor is 2 g/l or lower.6. Process according to claim 1 , wherein the remaining fraction after isolation of f) is kept as spent broth; and the spent broth is used in b).7. Process according claim 1 , wherein the cell is capable of consuming xylose in the lignocelluloic hydrolysate claim 1 , optionally substantially all xylose.8. Process according to claim 1 , wherein the cell is a yeast cell that is ...

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Номер записи: 52

24-03-2022 дата публикации

METHODS FOR PRODUCING ISOPROPANOL AND ACETONE IN A MICROORGANISM

Номер: US20220090045A1

Автор: Argyros Aaron, Kenealy William R., Stonehouse Emily

Принадлежит:

The present disclosure provides for novel metabolic pathways to increase acetone and isopropanol formation. More specifically, the present disclosure provides for a recombinant microorganism comprising a plurality of first native and/or heterologous enzymes that function in a first engineered metabolic pathway to convert fructose-6-phosphate to acetyl-CoA and acetate (e.g., phosphoketolase and acetate kinase), wherein the plurality of first native and/or heterologous enzymes is activated, upregulated, or overexpressed. The recombinant microorganism further comprises a plurality of second native and/or heterologous enzymes that function in a second engineered metabolic pathways to convert acetyl-CoA and acetate to isopropanol (e.g., thiolase, CoA transferase and acetoacetate decarboxylase), wherein the plurality of second native and/or heterologous enzymes is activated, upregulated, or overexpressed. Also provided are methods for making isopropanol or acetone using the recombinant microorganisms. 1. A recombinant microorganism comprising: a phosphoketolase; and', 'an acetate kinase; and, '(a) a plurality of first native and/or heterologous enzymes that function in a first engineered metabolic pathway to convert fructose-6-phosphate to acetyl-CoA and acetate, wherein the plurality of first native and/or heterologous enzymes is activated, upregulated, or overexpressed and comprises a thiolase;', 'a CoA transferase; and', 'an acetoacetate decarboxylase., '(b) a plurality of second native and/or heterologous enzymes that function in a second engineered metabolic pathways to convert acetyl-CoA and acetate to acetone and/or isopropanol, wherein the plurality of second native and/or heterologous enzymes is activated, upregulated, or overexpressed and comprises2. The recombinant microorganism of claim 1 , wherein the phosphoketolase:has the ability to convert D-xylulose 5-phosphate into D-glyceraldehyde 3-phosphate;has the ability to convert D-fructose 6-phosphate into D- ...

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Номер записи: 53

24-03-2022 дата публикации

METHOD FOR THE IN VIVO SYNTHESIS OF 4-HYDROXYMETHYLFURFURAL AND DERIVATIVES THEREOF

Номер: US20220090153A1

Автор: ALEXANDRINO Paulo Moises Raduan, GOUVEA Iuri Estrada

Принадлежит:

The present disclosure provides recombinant microorganisms and methods for the production of 4-HMF, 2,4-furandimethanol, furan-2,4-dicarbaldehyde, 4-(hydroxymethyl)furoic acid, 2-formylfuran-4-carboxylate, 4-formylfuran-2-carboxylate, and/or 2,4-FDCA from a carbon source. The method provides for engineered microorganisms that express endogenous and/or exogenous nucleic acid molecules that catalyze the conversion of a carbon source into 4-HMF, 2,4-furandimethanol, furan-2,4-dicarbaldehyde, 4-(hydroxymethyl)furoic acid, 2-formylfuran-4-carboxylate, 4-formylfuran-2-carboxylate, and/or 2,4-FDCA. The disclosure further provides methods of producing polymers derived from 4-HMF, 2,4-furandimethanol, furan-2,4-dicarbaldehyde, 4-(hydroxymethyl)furoic acid, 2-formylfuran-4-carboxylate, 4-formylfuran-2-carboxylate, and/or 2,4-FDCA. 175-. (canceled)76. A recombinant microorganism capable of producing 4-(hydroxymethyl)furoic acid from a feedstock comprising a carbon source , wherein the recombinant microorganism expresses the following:(a) endogenous or exogenous nucleic acid molecules encoding enzymes capable of converting a carbon source to glyceraldehyde 3-phosphate (G3P);(b) at least one endogenous or exogenous nucleic acid molecule encoding a (5-formylfuran-3-yl)methyl phosphate synthase that catalyzes the conversion of G3P from (a) to (5-formylfuran-3-yl)methyl phosphate;(c) at least one endogenous or exogenous nucleic acid molecule encoding a phosphatase that catalyzes the conversion of (5-formylfuran-3-yl)methyl phosphate from (b) to 4-hydroxymethylfurfural (4-HMF); and(d) at least one endogenous or exogenous nucleic acid molecule encoding a dehydrogenase, an oxidase, or a peroxygenase that catalyzes the conversion of 4-HMF from (c) to 4-(hydroxymethyl)furoic acid77. The recombinant microorganism of claim 76 , wherein the carbon source comprises a hexose claim 76 , a pentose claim 76 , glycerol claim 76 , CO claim 76 , sucroses or combinations thereof.78. The recombinant ...

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Номер записи: 54

16-03-2017 дата публикации

COMPOSITIONS OF ENZYMES STABILIZED WITH STABLE COENZYMES

Номер: US20170073661A1

Автор: Bucci Nadine, Heindl Dieter, Horn Carina, Meier Thomas, Nagel Rolf, Roedel Wolfgang, Schmuck Rainer, Steinke Nelli

Принадлежит:

Methods are provided for stabilizing an enzyme by storing the enzyme in the presence of a stabilized coenzyme. In addition, enzymes are provided that are stabilized with a stabilized coenzyme, as well as the use thereof in test elements for detecting analytes. Other aspects include unique compositions, methods, techniques, systems and devices involving enzyme stabilization. 1. A composition , comprising:an enzyme stabilized with a stabilized coenzyme, wherein the enzyme exhibits a decrease in enzyme activity of less than 50% based on an initial value of enzyme activity when stored for a period of time of at least 2 weeks in an environment that includes at least one of a temperature of at least 20° C. and a presence of light at a wavelength of ≧300 nm, and wherein the enzyme is selected from the group consisting of an alcohol dehydrogenase (EC I.1.1.2), a glucose-6-phosphate dehydrogenase (EC 1.1.1.49) and a diaphorase (EC1.6.99.2).2. The composition of claim 1 , wherein the decrease in enzyme activity is less than 30%.3. The composition of claim 1 , wherein the decrease in enzyme activity is less than 20% claim 1 ,4. The composition of claim 1 , wherein the period of time is at least four weeks.5. The composition of claim 1 , wherein the period of time is at least eight weeks.6. The composition of claim 1 , wherein the temperature is at least 25° C.7. The position of claim 1 , wherein the temperature is at least 30° C.8. The composition of claim 1 , wherein the enzyme further exhibits the decrease in enzyme activity of less than 50% when relative air humidity is at least 50%.9. The composition of claim 1 , wherein the enzyme further exhibits the decrease in enzyme activity of less than 50% in the absence of desiccants.10. The composition of claim 1 , wherein the enzyme further exhibits the decrease in enzyme activity of less than 50% in the absence of desiccants.16. The composition of claim 1 , wherein the stabilized coenzyme comprises carbaNAD.18. A detection ...

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Номер записи: 55

16-03-2017 дата публикации

PRODUCTION OF 1-PROPANOL

Номер: US20170073706A1

Автор: CHALABAEV Sabina, DUGAY Jose, GHIGO Jean-Marc, LETOFFE Sylvie

Принадлежит: INSTITUT PASTEUR

This invention encompasses methods of making 1-propanol. In some embodiments the methods comprise providing a cultured bacterial biofilm; culturing the bacterial biofilm under conditions suitable for production of 1-propanol; and collecting 1-propanol produced by the biofilm culture. In some embodiments the methods comprise providing a bacterial culture comprising bacteria and culture media, wherein the culture media comprises a concentration of threonine higher than that present in LB; maintaining the bacterial culture under conditions suitable for production of 1-propanol; and collecting 1-propanol produced by the culture. This invention also encompasses bacterial culture systems. In some embodiments the bacterial culture systems comprise a bacterial biofilm comprising bacteria growing on an artificial solid substrate; culture media; 1-propanol in liquid and/or gas form; and a collection device configured to collect 1-propanol produced by the culture. In come embodiments the culture systems comprise bacteria; culture media, wherein the culture media comprises a concentration of threonine higher than that present in LB; 1-propanol in liquid and/or gas form; and a collection device configured to collect 1-propanol produced by the culture. 1. A method of making 1-propanol , comprising the steps of:a) culturing bacteria under conditions suitable for expression of tdcA-G genes and adhE gene, thereby producing 1-propanol; andb) collecting 1-propanol produced by the culture.2. The method according to claim 1 , wherein step a) comprises providing a cultured bacterial biofilm.3. The method of claim 1 , wherein step a) comprises providing a planktonic bacterial culture.4. The method according to claim 1 , wherein said bacteria are cultured under anaerobic or microanaerobic conditions.5. The method according to claim 1 , wherein said bacteria are cultured in culture medium comprising threonine or a precursor thereof.6. The method according to claim 5 , wherein said culture ...

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Номер записи: 56

19-03-2015 дата публикации

GENETICALLY ENGINEERED YEAST CELLS

Номер: US20150079646A1

Автор: Chen Yun, Daviet Laurent, Nielsen Jens, Schalk Michel, Siewers Verena

Принадлежит: FIRMENICH SA

The present invention relates to yeast cells producing high levels of acetoacetyl-CoA. It also relates to a method for making such yeast cells and to the use of such yeast cells in a method for producing acetyl-CoA derived products. 1. A yeast cell modified by overexpression of an aldehyde dehydrogenase and an acetyl-CoA synthetase (ACS) , characterized in that an alcohol dehydrogenase and an acetoacetyl-CoA synthase are further overexpressed.2. A yeast cell according to claim 1 , characterized in that a gene encoding a peroxisomal citrate synthase or a gene encoding a cytosolic malate synthase have been deleted from the yeast cell genome.3Saccharomyces cerevisiae. A yeast cell according to claim 1 , characterized in that said yeast cell is a cell.4. A yeast cell according to claim 3 , characterized in that the aldehyde dehydrogenase is ALD6 claim 3 , the alcohol dehydrogenase is ADH2 claim 3 , the acetoacetyl-CoA synthase is ERG10 claim 3 , the peroxisomal citrate synthase is CIT2 and the cytosolic malate synthase is MLS1.5. A yeast cell according to claim 1 , characterized in that the ACS contains a point mutation that prevents the enzyme from being inhibited by acetylation.6Salmonella enterica.. A yeast cell according to claim 5 , characterized in that the ACS is the variant L614P from7. A method of making a modified yeast cell comprising over-expressing an aldehyde dehydrogenase and an acetyl-CoA synthase (ACS) claim 5 , characterized in that said method further comprises over-expressing an alcohol dehydrogenase and an acetoacetyl-CoA synthase.8. A method according to claim 7 , characterized in that the method further comprises deleting a gene encoding a peroxisomal citrate synthase or a gene encoding a cytosolic malate synthase from the yeast cell genome.9Saccharomyces cerevisiae. A method according to claim 7 , characterized in that said yeast cell is a cell.10. A method according to claim 9 , characterized in that the aldehyde dehydrogenase is ALD6 claim 9 , ...

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Номер записи: 57

19-03-2015 дата публикации

Recombinant microorganisms exhibiting increased flux through a fermentation pathway

Номер: US20150079650A1

Автор: Koepke Michael, Mueller Alexander Paul

Принадлежит:

The invention provides methods of increasing the production of fermentation products by increasing flux through a fermentation pathway by optimising enzymatic reactions. In particular, the invention relates to identifying enzymes and/or co-factors involved in metabolic bottlenecks in fermentation pathways, and fermenting a CO-comprising substrate with a recombinant carboxydotrophic Clostridia microorganism adapted to exhibit increased activity of the one or more of said enzymes, or increased availability of the one or more of said co-factors, when compared to a parental microorganism. 1. A method of producing a fermentation product , the method comprising:a) determining a rate-limiting pathway reaction in a fermentation pathway;b) identifying at least one enzyme, co-factor or both, which are involved in catalysing the rate-limiting pathway reaction;c) fermenting a CO-comprising substrate with a recombinant carboxydotrophic Clostridia microorganism adapted to exhibit at least one of: i) increased activity of the at least one enzyme of (b) or a functionally equivalent variant thereof, or ii) increased availability of the at least one co-factor of (b), when compared to a parental microorganism, to produce a fermentation product.2. A method of producing a fermentation product , the method comprising fermenting a CO-comprising substrate with a recombinant carboxydotrophic Clostridia microorganism to produce a fermentation product , wherein the recombinant microorganism is adapted to exhibit at least one of:a) increased activity of at least one enzyme identified as being involved in catalysing a rate-limiting pathway reaction of a fermentation pathway, or a functionally equivalent variant thereof, when compared to a parental microorganism; orb) increased availability of at least one co-factor identified as being involved in catalysing a rate-limiting pathway reaction of a fermentation pathway, when compared to a parental microorganism.4. A recombinant carboxydotrophic ...

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Номер записи: 58

22-03-2018 дата публикации

TRICHOTHECENE-TRANSFORMING ALCOHOL DEHYDROGENASE, METHOD FOR TRANSFORMING TRICHOTHECENES AND TRICHOTHECENE-TRANSFORMING ADDITIVE

Номер: US20180080011A1

Автор: BERNARD Claudia, BINDER Eva-Maria, WEBER Barbara

Принадлежит: Erber Aktiengesellschaft

A alcohol dehydrogenase of sequence ID no. 1 containing metal ions and a quinone cofactor, or in addition, a functional variant exhibiting a sequence identity of at least 80%, preferably at least 86%, especially preferred at least 89% and at least one redox cofactor for the transformation of at least one trichothecene exhibiting a hydroxyl group on the C-3 atom, as well as a method for the enzymatic transformation of trichothecenes and a trichothecene-transforming additive. 1. A alcohol dehydrogenase of SEQ ID no. 1 containing metal ions and a quinone cofactor , or in addition , a functional variant exhibiting a sequence identity of at least 80% , and at least one redox cofactor for the transformation of at least one trichothecene exhibiting a hydroxyl group on the C-3 atom.2. The alcohol dehydrogenase according to claim 1 , wherein the amino acid sequence of the functional variant is selected from the group of sequence ID numbers 2 to 4.3. The alcohol dehydrogenase according to claim 1 , wherein the quinone cofactor is selected from the group PCC claim 1 , TTC claim 1 , TPC claim 1 , LTC claim 1 , and CTC.4. The alcohol dehydrogenase according to claim 1 , wherein the quinone cofactor is bound to the alcohol dehydrogenase by at least one metal ion selected from the group Li claim 1 , Na claim 1 , K claim 1 , Mg claim 1 , Ca claim 1 , Zn claim 1 , Zn claim 1 , Mn claim 1 , Mn claim 1 , Fe claim 1 , Fe claim 1 , Cu claim 1 , Cu claim 1 , Co and Co claim 1 , preferably Ca and Mg.5. The alcohol dehydrogenase according to claim 1 , wherein at least one redox cofactor is selected from the group PMS claim 1 , PMS derivatives claim 1 , potassium hexacyanoferrate (III) claim 1 , sodium hexacyanoferrate (III) claim 1 , cytochrome C claim 1 , coenzyme Q1 claim 1 , coenzyme Q10 claim 1 , methylene blue claim 1 , and TMPD claim 1 , preferably PMS claim 1 , coenzyme Q1 and coenzyme Q10.6. The alcohol dehydrogenase according to claim 1 , wherein the transformation of ...

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Номер записи: 59

22-03-2018 дата публикации

BIOSYNTHETIC PATHWAYS AND METHODS

Номер: US20180080032A1

Автор: Xiong Mingyong, Zhang Kechun

Принадлежит:

This disclosure describes a recombinant microbial cells and methods of making and using such recombinant microbial cells. Generally, the recombinant cells may be modified to exhibit increased biosynthesis of a TCA derivative compared to a wild-type control. In some embodiments, the TCA derivative can include 1,4-butanediol. In various embodiments, the microbial cell is a fungal cell or a bacterial cell. In some embodiments, the increased biosynthesis of the TCA derivative can include an increase in xylose dehydrogenase activity, xylonolactonase activity, xylonate dehydratase activity, or 2-keto-3-deoxyaldonic acid dehydratase activity. 1. A recombinant microbial cell modified to exhibit increased biosynthesis of a TCA derivative compared to a wild-type control.2134-. (canceled) This application claims priority to U.S. Provisional Patent Application Ser. No. 61/738,752, filed Dec. 18, 2012 and U.S. Provisional Patent Application Ser. No. 61/821,490, filed May 9, 2013, each of which is incorporated herein by reference.This disclosure describes, in one aspect, a recombinant microbial cell modified to exhibit increased biosynthesis of a TCA derivative compared to a wild-type control. In some embodiments, the TCA derivative can include 1,4-butanediol. In various embodiments, the microbial cell is a fungal cell or a bacterial cell. In some embodiments, the increased biosynthesis of the TCA derivative can include an increase in xylose dehydrogenase activity, xylonolactonase activity, xylonate dehydratase activity, or 2-keto-3-deoxyaldonic acid dehydratase activity.In another aspect, this disclosure describes a method that generally includes incubating any embodiments of the recombinant cell summarized above in medium that includes a carbon source under conditions effective for the recombinant cell to produce a TCA derivative. In some embodiments, the TCA derivative can include 1,4-butanediol. In some embodiments, the carbon source can include xylose, arabinose, glucaric ...

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Номер записи: 60

31-03-2022 дата публикации

RECOMBINANT MICROORGANISMS AND USES THEREFOR

Номер: US20220098560A1

Автор: Juminaga Darmawi, Nogle Robert, Shockley Arthur

Принадлежит:

Provided is a genetically engineered microorganism comprising expression of multiple CoA transferases conferring certain advantages, including increased product production and fermentation stability. Also provided is a method for increasing production of a product comprising culturing the genetically engineered microorganism in the presence of a gaseous substrate wherein the gaseous substrate may comprise a C1-carbon source comprising one or more of CO, CO, and H. 1. A genetically engineered Wood-Ljungdahl microorganism comprising a first exogenous CoA transferase , and at least one additional exogenous CoA transferase.2. The microorganism of claim 1 , wherein the first exogenous CoA transferase replaces a coding region of an acetolactate decarboxylase gene.3. The microorganism of claim 1 , wherein the at least one additional exogenous CoA transferase replaces a coding region of an aldehyde-alcohol dehydrogenase gene.4. The microorganism of claim 1 , further comprising an exogenous thiolase and an exogenous decarboxylase selected from acetoacetate decarboxylase or alpha-ketoisovalerate decarboxylase claim 1 , or any combination thereof.5. The microorganism of claim 4 , wherein the exogenous thiolase and the exogenous decarboxylase selected from acetoacetate decarboxylase or alpha-ketoisovalerate decarboxylase claim 4 , or any combination thereof claim 4 , function with the first exogenous CoA transferase or function with the least one additional exogenous CoA transferase.6Clostridium acetobutylicumClostridium beijerinckii. The microorganism of claim 1 , wherein the exogenous CoA transferases are CtfA and CtfB claim 1 , or CtfA and CtfB.7. The microorganism of claim 6 , wherein the exogenous CoA transferases are nonnative to the microorganism.8. The microorganism of claim 6 , wherein the exogenous CoA transferases are native to the microorganism.9. The microorganism of claim 6 , wherein the exogenous CoA transferases are the same.10. The microorganism of claim 6 , ...

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Номер записи: 61

29-03-2018 дата публикации

ENGINEERING OF MULTI-CARBON SUBSTRATE UTILIZATION PATHWAYS IN METHANOTROPHIC BACTERIA

Номер: US20180087059A1

Автор: Leonard Effendi, Saville Renee M., Silverman Joshua A.

Принадлежит:

The present disclosure relates to genetically engineered methanotrophic bacteria with the capability of growing on a multi-carbon substrate (e.g., glycerol) as a primary or sole carbon source and methods for growing methanotrophic bacteria on the multi-carbon substrate. 1. A recombinant methanotrophic bacterium , comprising an exogenous polynucleotide encoding at least two glycerol utilization pathway components , wherein a first encoded glycerol utilization pathway component comprises a glycerol kinase and a second encoded glycerol utilization pathway component comprises a glycerol-3-phosphate dehydrogenase (G3PDH);wherein the recombinant methanotrophic bacterium expresses an amount of glycerol kinase and G3PDH sufficient to permit utilization of glycerol as a primary carbon source as compared to an unmodified parent methanotrophic bacterium; and{'i': Methylococcus capsulatus, Methylomonas', 'Methylosinus trichosporium, Methylosinus sporium, Methylocystis parvus, Methylomonas methanica, Methylomonas albus, Methylobacter capsulatus, Methylomonas flagellata, Methylacidiphilum infernorum, Methylomicrobium alcaliphilum Methylocella silvestris, Methylocella palustris, Methylocella tundrae, Methylocystis daltona, Methylocystis bryophila', 'Methylocapsa aurea., 'wherein the methanotrophic bacterium is selected from sp. 16A, , or'}2Escherichia coli, Acinetobacter baumannii, Fusobacterium nucleatumvincentii, PantoeaPseudomonas aeruginosa, Shigella flexneri, Shewanella balticaActinobacillus pleuropneumoniasSalmonella entericaentericaYersinia bercovieri, Aeromonas veroniiPseudomonas fluorescens, SerratiaVibrio fischeriHaemophilus haemolyticus, Vibrio harveyi, Vibrio cholera, Pseudomonas putidaPectobacterium carotovorumcarotovorumPseudomonas syringae, Acinetobacter, Photobacterium profundumCitrobacter freundii, Klebsiella pneumoniae, EnterobacterEnterococcus casseliflavus, Enterococcus faecalis, Bacillus stearothermophilus, Bacillus subtilis, Streptococcus pyogenes, ...

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Номер записи: 62

29-03-2018 дата публикации

Use of Bacteriocin-Producing Ethanologens In Biofuel Production

Номер: US20180087073A1

Автор: Broadbent Jeffrey, Phrommao Ekkarat, Steele James L.

Принадлежит:

An ethanologen for producing biofuel from one or more carbohydrates and reducing lactate and acetate production in a biofuel manufacturing process. The ethanologen is made by introducing into the ethanologen one or more exogenous genes required for production of a bacteriocin. The resulting ethanologen reduces lactate and acetate production by contaminant lactic acid bacteria by expression of the bacteriocin during the biofuel manufacturing process. Certain resulting ethanologens ferment sugars not naturally or not preferentially utilized by during the manufacturing process 1. An ethanologen for inhibiting contaminant lactic acid bacteria present in a biofuel manufacturing process , comprising an ethanologen containing one or more exogenous genes required for production of a bacteriocin , wherein production of the bacteriocin by the ethanologen inhibits contaminant lactic acid bacteria present in the biofuel manufacturing process.2. The ethanologen of claim 1 , wherein inhibition of the contaminant lactic acid bacteria results in reduced lactate and acetate levels in the biofuel manufacturing process.3. The ethanologen of claim 1 , wherein the ethanologen is capable of fermenting sugars not naturally or not preferentially utilized by a main fermenting microbe present in the biofuel manufacturing process.4Saccharomyces cerevisiae.. The ethanologen of claim 3 , wherein the main fermenting microbe is5. The ethanologen of claim 1 , wherein the ethanologen is a native biofuel-producing organism.6SacchoromycesZymononas. The ethanologen of claim 5 , wherein the native biofuel-producing organism is sp. or sp.7. The ethanologen of claim 1 , wherein the ethanologen is an organism engineered to produce the biofuel.8EscherichiaClostridium. The ethanologen of claim 7 , wherein the organism engineered to produce the biofuel is a lactic acid bacterium claim 7 , sp. claim 7 , or sp.9LactobacillusLactococcusEnterococcusStreptococcus. The ethanologen of claim 8 , wherein the lactic ...

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Номер записи: 63

29-03-2018 дата публикации

MICROORGANISMS AND METHODS FOR PRODUCING PYRUVATE, ETHANOL, AND OTHER COMPOUNDS

Номер: US20180087074A1

Автор: Reed Jennifer L., Zhang Xiaolin

Принадлежит: WISCONSIN ALUMNI RESEARCH FOUNDATION

Microorganisms comprising modifications for producing pyruvate, ethanol, and other compounds. The microorganisms comprise modifications that reduce or ablate activity of one or more of pyruvate dehydrogenase, 2-oxoglutarate dehydrogenase, phosphate acetyltransferase, acetate kinase, pyruvate oxidase, lactate dehydrogenase, cytochrome terminal oxidase, succinate dehydrogenase, 6-phosphogluconate dehydrogenase, glutamate dehydrogenase, pyruvate formate lyase, pyruvate formate lyase activating enzyme, and isocitrate lyase. The microorganisms optionally comprise modifications that enhance expression or activity of pyruvate decarboxylase and alcohol dehydrogenase. The microorganisms are optionally evolved in defined media to enhance specific production of one or more compounds. Methods of producing compounds with the microorganisms are provided. 120-. (canceled)21. A microorganism comprising activity-reducing or activity-ablating mutations in endogenous genes encoding:one or more enzymes in a first set selected from the group consisting of pyruvate dehydrogenase and 2-oxoglutarate dehydrogenase;one or more enzymes in a second set selected from the group consisting of phosphate acetyltransferase, acetate kinase, and pyruvate oxidase; and lactate dehydrogenase and one or more enzymes selected from the group consisting of cytochrome terminal oxidase and succinate dehydrogenase; or', 'one or more enzymes selected from the group consisting of cytochrome terminal oxidase and succinate dehydrogenase and one or more enzymes selected from the group consisting of 6-phosphogluconate dehydrogenase and glutamate dehydrogenase., 'enzymes in a third set comprising22. The microorganism of claim 21 , wherein the one or more enzymes in the first set is pyruvate dehydrogenase.23. The microorganism of claim 21 , wherein the one or more enzymes in the second set are selected from the group consisting of phosphate acetyltransferase and pyruvate oxidase.24. The microorganism of claim 21 , ...

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Номер записи: 64

02-04-2015 дата публикации

Microorganism capable of producing 1,4-butanediol and method of producing 1,4-butanediol using the same

Номер: US20150093798A1

Автор: Hwayoung Cho, Jinhwan Park, Kwangmyung Cho, Yukyung JUNG

Принадлежит: SAMSUNG ELECTRONICS CO LTD

A microorganism capable of producing 1,4-butanediol and a method of producing 1,4-butanediol using the same.

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Номер записи: 65

30-03-2017 дата публикации

XYLOSE UTILIZING OLEAGINOUS YEAST

Номер: US20170088866A1

Автор: GUARNIERI Michael T., KNOSHAUG Eric P., SINGH Arjun, ZHANG Min

Принадлежит:

Presented herein are oleaginous strains of yeast such as that have been modified to allow for xylose utilization. Such strains are also modified to allow for higher lipid accumulation utilizing a broad range of sugar monomers such as those released during pretreatment and enzymatic saccharification of lignocellulosic biomass. Methods of producing lipids and ethanol using these yeast strains are also disclosed. 1. An engineered yeast cell , comprising exogenously added genes encoding xylose reductase , xylitol dehydrogenase and xylulose kinase enzymes; wherein the snfl gene of the yeast cell has been ablated; and wherein the yeast cell has been modified to express the GAL2 transporter in the presence of glucose.2. The yeast cell of claim 1 , further comprising a genetic modification that allows for overexpression of a diacylglycerol acyltransferase.3Saccharomyces.. The yeast cell of claim 2 , wherein the yeast cell is from of strain of the genus4Saccharomyces cerevisiae.. The yeast cell of claim 3 , wherein the yeast cell is from of strain of5Pichia stipitis.. The yeast cell of claim 2 , wherein the xylose reductase and xylitol dehydrogenase enzymes are from6. The yeast cell of claim 5 , wherein the xylose reductase and xylitol dehydrogenase enzymes are XYL1 and XYL2.7S. cerevisiae. The yeast cell of claim 2 , wherein the xylulose kinase enzyme is XKS1.8. The yeast cell of claim 2 , wherein the modification to express the GAL2 transporter in the presence of glucose is the ablation of at least one copy of a gene encoding a GAL80 protein.9. The yeast cell of claim 4 , wherein the cell is from the strain BFY709.10S. cerevisiaeL. starkeyi.. The yeast cell of claim 2 , wherein the diacylglycerol acyltransferase is DGA1 from or11. The yeast cell of claim 10 , wherein the cell is from the strain BFY742 or BFY748.12. The yeast cell of claim 2 , wherein the yeast cell accumulates at least 25% dcw lipids when cultured in the presence of sugars.13. The yeast cell of claim 12 , ...

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Номер записи: 66

05-05-2022 дата публикации

FERMENTATIVE GLYCEROL-FREE ETHANOL PRODUCTION

Номер: US20220135989A1

Автор: GUADALUPE MEDINA Victor Gabriel, Pronk Jacobus Thomas, VAN MARIS Antonius Jeroen Adriaan

Принадлежит:

The present invention relates to a yeast cell, in particular a recombinant yeast cell, the cell lacking enzymatic activity needed for the NADH-dependent glycerol synthesis or the cell having a reduced enzymatic activity with respect to the NADH-dependent glycerol synthesis compared to its corresponding wild-type yeast cell, the cell comprising one or more heterologous nucleic acid sequences encoding an NAD-dependent acetylating acetaldehyde dehydrogenase (EC 1.2.1.10) activity. The invention further relates to the use of a cell according to the invention in the preparation of ethanol.

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Номер записи: 67

19-03-2020 дата публикации

CATALYTICALLY ACTIVE PROTEIN AGGREGATES AND METHODS FOR PRODUCING THE SAME

Номер: US20200087355A1

Автор: Diener Martin, Jaeger Karl-Erich, Krauss Ulrich, Pohl Martina

Принадлежит:

Disclosed are catalytically active water-insoluble protein aggregates comprising fusion proteins which comprise a coiled-coil domain and a catalytic domain, methods of manufacturing such protein aggregates, and their use. 1. A water-insoluble protein aggregate comprising a fusion protein , wherein said fusion protein comprises a coiled-coil domain and a catalytic domain.2. The protein-aggregate according to claim 1 , wherein the fusion protein is a N-terminal fusion protein.3. The protein aggregate according to claim 1 , wherein the fusion protein is a C-terminal fusion protein.4. The protein aggregate according to claim 1 , wherein the coiled-coil domain is selected from the group consisting ofdomains comprising at least three heptad repeats, the amino acid sequence of said heptad repeats being represented by hxxhcxc, wherein h represents a hydrophobic amino acid residue, c represents a charged amino acid residue, and x represents any amino acid residue;domains wherein the PCOILS webserver predicts the heptad repeat pattern hxxhcxc, wherein h represents a hydrophobic amino acid residue, c represents a charged amino acid residue, and x represents any amino acid residue, in any of the 3 sequence windows (14, 21, 28 amino acids) with a score above 0.2 over 3 heptad repeats, using the MTIDK matrix;domains wherein the MARCOILS webserver predicts at least 1 coiled-coil domain with a threshold of 1.0 within the sequence, using the 9FAM matrix; anddomains comprising a repeat pattern comprising two or more hydrophobic amino acid residues and two or more charged amino acid residues within a stretch of seven amino acid residues, wherein the PCOILS webserver predicts the repeat pattern comprising two or more hydrophobic amino acid residues and two or more charged amino acid residues within a stretch of seven amino acid residues with a score above 0.2 over two repeats in any of the three sequence windows (14, 21, 28 amino acids).5. The protein aggregate according to claim 1 , ...

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Номер записи: 68

28-03-2019 дата публикации

METHOD OF PRODUCING THIN ENZYME-BASED SENSING LAYERS ON PLANAR SENSORS

Номер: US20190090796A1

Автор: CHEN Samson, Scherer Axel

Принадлежит:

A sensor implanted in tissues and including a sensing layer is fabricated by mixing the signal transduction enzyme with non-reactive components including buffer salts and fillers, and spin coating the enzyme onto a substrate. The signal transduction enzyme is crosslinked by introducing the coated substrate in a vacuum chamber. In the chamber, a crosslinker evaporates and is deposited onto the enzyme, therefore crosslinking the enzyme. 1. A method comprising:coating a signal transduction enzyme onto a substrate, the signal transduction enzyme being configured to detect a molecular compound; andcrosslinking the signal transduction enzyme by depositing a crosslinker onto the signal transduction enzyme.2. The method of claim 1 , wherein coating the signal transduction enzyme onto the substrate is by spin coating.3. The method of claim 2 , further comprising claim 2 , prior to spin coating the signal transduction enzyme onto the substrate: mixing the signal transduction enzyme in a solvent with at least one non-reactive component.4. The method of claim 3 , wherein the at least one non-reactive component comprises a filler claim 3 , a buffer salt to regulate pH claim 3 , or both the filler and the buffer salt.5. The method of claim 4 , wherein the crosslinking the signal transduction enzyme is by:placing the substrate, coated with the signal transduction enzyme, and the crosslinker in a vacuum chamber; andreducing a pressure within the vacuum chamber, thereby causing the crosslinker to vaporize and deposit onto the signal transduction enzyme.6. The method of claim 5 , wherein the spin coated signal transduction enzyme is less than 1 micrometer thick.7. The method of claim 6 , wherein the solvent is water.8. The method of claim 7 , wherein the signal transduction enzyme is an oxidase enzyme.9Escherichia coliPenicillium. The method of claim 8 , wherein the signal transduction enzyme is selected from the group consisting of: malate oxidase claim 8 , EC 1.1.3.3 claim 8 , ...

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Номер записи: 69

01-04-2021 дата публикации

GENETICALLY MODIFIED MICROORGANISMS HAVING IMPROVED TOLERANCE TOWARDS L-SERINE

Номер: US20210095245A1

Автор: Mundhada Hemanshu, Nielsen Alex Toftgaard

Принадлежит:

The present invention generally relates to the microbiological industry, and specifically to the production of L-serine or L-serine derivatives using genetically modified bacteria. The present invention provides genetically modified microorganisms, such as bacteria, wherein the expression of genes encoding for enzymes involved in the degradation of L-serine is attenuated, such as by inactivation, which makes them particularly suitable for the production of L-serine at higher yield. The present invention also provides means by which the microorganism, and more particularly a bacterium, can be made tolerant towards higher concentrations of serine. The present invention also provides methods for the production of L-serine or L-serine derivative using such genetically modified microorganisms. 1. (canceled)2Escherichia coli. An bacterium which expresses a polypeptide having an amino acid sequence , which has at least 90% sequence identity to the amino acid sequence set forth in SEQ ID NO: 12 , wherein in said amino acid sequence at position 143 D is replaced by another amino acid.3. The bacterium according to which expresses a polypeptide having an amino acid sequence claim 2 , which has at least 90% sequence identity to the amino acid sequence set forth in SEQ ID NO: 12 claim 2 , wherein in said amino acid sequence at position 143 D is replaced by G.4. The bacterium according to claim 2 , wherein said bacterium comprises within the lrp gene one of more nucleotide substitutions resulting in an amino acid substitution in the encoded polypeptide at position 143.5. The bacterium according to claim 2 , wherein at least one gene selected from sdaA claim 2 , sdaB claim 2 , tdcG or glyA is inactivated.6. The bacterium according to claim 2 , wherein at least two genes selected from sdaA claim 2 , sdaB claim 2 , tdcG or glyA are inactivated.7. The bacterium according to claim 2 , wherein at least three genes selected from sdaA claim 2 , sdaB claim 2 , tdcG or glyA are inactivated ...

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Номер записи: 70

14-04-2016 дата публикации

Consumable Cryopreserved Cells Transiently Overexpressing Gene(s) Encoding Drug Transporter Protein(s) and/or Drug Metabolizing Enzyme(s)

Номер: US20160102131A1

Автор: Christopher J. Patten, Jie Wang, NA Li

Принадлежит: Corning Inc

The present invention discloses cryopreserved recombinant cells for screening drug candidates that transiently overexpress one or more drug transporter proteins and/or drug metabolizing enzymes. Advantageously, such cells provide a cost-efficient consumable product that streamlines the process of screening whether drug candidates are substrates or inhibitors of drug transporter proteins and/or drug metabolizing enzymes.

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Номер записи: 71

26-03-2020 дата публикации

IMPROVED YEAST STRAINS FOR ETHANOL PRODUCTION

Номер: US20200095536A1

Автор: PAPAPETRIDIS Ioannis, Pronk Jacobus Thomas, VAN MARIS Antonius Jeroen Adriaan

Принадлежит:

This invention relates to a recombinant cell, preferably a recombinant yeast cell comprising: a) a gene coding for an enzyme having glycerol-3-phosphate dehydrogenase activity, wherein said enzyme has a cofactor dependency for at least NADP and/or for NADPH; b) a gene encoding an enzyme having at least NAD dependent acetylating acetaldehyde dehydrogenase activity (EC 1.2.1.10); and c) a mutation or disruption in at least one gene selected from the group of GPD1 and GPD2. Said cell is suitable for ethanol production, has a reduced glycerol production at high ethanol yield. 1. A recombinant cell , optionally a recombinant yeast cell comprising:{'sup': '+', 'a) a gene coding for an enzyme having glycerol-3-phosphate dehydrogenase activity, wherein said enzyme has a cofactor dependency for at least NADP and/or for NADPH;'}{'sup': '+', 'b) a gene encoding an enzyme having at least NAD dependent acetylating acetaldehyde dehydrogenase activity (EC 1.2.1.10); and'}c) a mutation or disruption in at least one gene selected from the group of GPD1 and GPD2.2. The cell according to wherein the enzyme having glycerol-3-phosphate dehydrogenase activity has a higher affinity and/or lower Michaelis constant and/or a higher maximum activity for NADPH than for NADH.3. The cell according to wherein the gene coding for an enzyme having glycerol-3-phosphate dehydrogenase activity comprises at least one exogenous gene.4. The cell according to wherein said gene encodes an enzyme with an amino acid sequence according to SEQ ID NO: 1 or a functional homologue thereof having a sequence identity of at least 50%.5. The cell according to wherein the gene encoding an enzyme having at least NAD dependent acetylating acetaldehyde dehydrogenase activity encodes an enzyme with an amino acid sequence according to SEQ ID NO: 2 or a functional homologue thereof having a sequence identity of at least 50%.6. The cell according to which cell is free of claim 1 , or has reduced NADPH-dependent aldehyde ...

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Номер записи: 72

12-04-2018 дата публикации

MODIFIED MICROORGANISM FOR THE OPTIMIZED PRODUCTION OF 2,4-DIHYDROXYBUTYRATE

Номер: US20180100169A1

Автор: BESTEL-CORRE Gwénaëlle, Dumon-Seignovert Laurence, Soucaille Philippe

Принадлежит: METABOLIC EXPLORER

The present invention relates to a genetically modified microorganism for the production of 2,4-dihydroxybutyrate, by metabolic transformation of xylose via the 1,2,4-butanetriol intermediate. The invention also relates to a method for the production of 2,4-dihydroxybutyrate by culturing said genetically modified microorganism in a fermentation medium and recovering 2,4-DHB from said medium. 1. A microorganism genetically modified for the production of 2 ,4-dihydroxybutyrate by converting xylose into 1 ,2 ,4-butanetriol , wherein said microorganism is further genetically modified for:i) oxidizing 1,2,4-butanetriol into 2,4-dihydroxybutanal; andii) oxidizing 2,4-dihydroxybutanal into 2,4-dihydroxybutyrate.2. The microorganism according to claim 1 , wherein the genetic modification i) is an overexpression of at least one gene encoding an oxidoreductase acting on the CH—OH group of donors.3. The microorganism according to claim 2 , wherein said oxidoreductase is selected from the group consisting of alcohol dehydrogenases claim 2 , lactaldehyde reductases claim 2 , glyoxylate reductases claim 2 , didehydrogluconate reductases claim 2 , and any combination thereof.4. The microorganism according to claim 1 , wherein the genetic modification ii) is an overexpression of at least one gene encoding an oxidoreductase acting on the aldehyde or oxo group of donors.5. The microorganism according to claim 4 , wherein said oxidoreductase is selected from the group consisting of an aldehyde dehydrogenase claim 4 , an aldehyde oxidase claim 4 , and any combination thereof.6. The microorganism according to claim 1 , wherein the genetic modification for converting xylose into 1 claim 1 ,2 claim 1 ,4-butanetriol is an overexpression of at least one of the following genes:a gene encoding a xylose dehydrogenase,a gene encoding a xylonolactonase,a gene encoding a xylonate dehydratase,a gene encoding a 3-deoxy-D-glycero-pentulosonate decarboxylase,a gene encoding a 1,2,4-butanetriol ...

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Номер записи: 73

02-06-2022 дата публикации

Bacteria engineered to treat disorders involving the catabolism of a branched chain amino acid

Номер: US20220168362A1

Автор: Alex TUCKER, Dean Falb, Jonathan W. Kotula, Paul F. Miller, Vincent M. Isabella, Yves Millet

Принадлежит: Synlogic Inc, Synlogic Operating Co Inc

The present disclosure provides recombinant bacterial cells that have been engineered with genetic circuitry which allow the recombinant bacterial cells to sense a patient's internal environment and respond by turning an engineered metabolic pathway on or off. When turned on, the recombinant bacterial cells complete all of the steps in a metabolic pathway to achieve a therapeutic effect in a host subject. These recombinant bacterial cells are designed to drive therapeutic effects throughout the body of a host from a point of origin of the microbiome. Specifically, the present disclosure provides recombinant bacterial cells comprising a heterologous gene encoding a branched chain amino acid catabolism enzyme. The disclosure further provides pharmaceutical compositions comprising the recombinant bacteria, and methods for treating disorders involving the catabolism of branched chain amino acids using the pharmaceutical compositions disclosed herein.

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Номер записи: 74

04-04-2019 дата публикации

Host Cells and Methods for Production of Isobutanol

Номер: US20190100777A1

Автор: ANTHONY LARRY CAMERON, Maggio-Hall Lori Ann

Принадлежит:

The invention relates to recombinant host cells having at least one integrated polynucleotide encoding a polypeptide that catalyzes a step in a pyruvate-utilizing biosynthetic pathway, e.g., pyruvate to acetolactate conversion. The invention also relates to methods of increasing the biosynthetic production of isobutanol, 2,3-butanediol, 2-butanol or 2-butanone using such host cells. 1. A recombinant host cell comprising:{'i': Bacillus subtilis, Klebsiella pneumonia, Lactococcus lactis, Staphylococcus aureus, Listeria monocytogenes, Streptococcus mutans, Streptococcus thermophiles, Vibrio angustum', 'Bacillus cereus;, '(a) a polynucleotide encoding a polypeptide which catalyzes the substrate to product conversion of pyruvate to acetolactate wherein the polypeptide is an acetolactate synthase from , or'}(b) a polynucleotide encoding a polypeptide which catalyzes the substrate to product conversion of acetolactate to 2,3-dihydroxyisovalerate wherein the polypeptide is a ketol-acid reductoisomerase and the ketol-acid reductoisomerase has at least 95% identity to SEQ ID NO: 224;{'i': Escherichia coli, Bacillus subtilis, Methanococcus maripaludis', 'Streptococcus mutans;, '(c) a polynucleotide encoding a polypeptide which catalyzes the substrate to product conversion of 2,3-dihydroxyisovalerate to α-ketoisovalerate wherein the polypeptide is a dihydroxyacid dehydratase from , or'}(d) a polynucleotide encoding a polypeptide which catalyzes the substrate to product conversion of α-ketoisovalerate to isobutyraldehyde wherein the polypeptide is a branched-chain α-keto acid decarboxylase and the branched-chain α-keto acid decarboxylase has at least 95% identity to SEQ ID NO: 48; and{'i': Achromobacter xylosoxidans', 'Beijerinkia indica,, '(e) a polynucleotide encoding a polypeptide which catalyzes the substrate to product conversion isobutyraldehyde to isobutanol wherein the polypeptide is an alcohol dehydrogenase from or'}wherein expression of pyruvate decarboxylase in the ...

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Номер записи: 75

02-06-2022 дата публикации

MUT- METHYLOTROPHIC YEAST

Номер: US20220170032A1

Автор: GASSER Brigitte, MATTANOVICH Diethard, ZAVEC Domen

Принадлежит:

A recombinant methanol utilization pathway deficient methylotrophic yeast (Mut-) host cell which is engineered: a) by one or more genetic modifications to reduce expression of a first and a second endogenous gene compared to the host cell prior to said one or more genetic modifications, wherein i. the first endogenous gene encodes alcohol oxidase 1 (AOX1) comprising the amino acid sequence identified as SEQ ID NO:1 or a homologue thereof, and ii. the second endogenous gene encodes alcohol oxidase 2 (AOX2) comprising the amino acid sequence identified as SEQ ID NO:3 or a homologue thereof, and b) by one or more genetic modifications to increase expression of an alcohol dehydrogenase (ADH2) gene compared to the host cell prior to said one or more genetic modifications, wherein the ADH2 gene encodes an alcohol dehydrogenase (ADH2). 2. The Mut− host cell of claim 1 , wherein said one or more genetic modifications comprise a disruption claim 1 , substitution claim 1 , deletion claim 1 , knockin or knockout of (i) one or more polynucleotides claim 1 , or a part thereof; or (ii) an expression control sequence.3. The Mut− host cell of claim 2 , wherein said expression control sequence is selected from the group consisting of a promoter claim 2 , a ribosomal binding site claim 2 , transcriptional or translational start and stop sequences claim 2 , an enhancer and an activator sequence.4. The Mut− host cell of claim 1 , wherein said first and/or second endogenous gene is knocked out by said one or more genetic modifications.5. The Mut− host cell of claim 1 , wherein the ADH2 gene is endogenous or heterologous to the Mut− host cell.6. The Mut− host cell of claim 1 , wherein the ADH2 is any one of:{'i': 'P. pastoris', 'a) ADH2 comprising the amino acid sequence of SEQ ID NO:50, or a homologue thereof that is endogenous to a yeast species; or'}b) a mutant of the ADH2 of a), which is at least 60% identical to SEQ ID NO:50.7. The Mut− host cell of claim 1 , wherein said one or ...

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Номер записи: 76

02-06-2022 дата публикации

USE OF BACTERIOCIN-PRODUCING ETHANOLOGENS IN BIOFUEL PRODUCTION

Номер: US20220170052A1

Автор: Broadbent Jeffrey, Phrommao Ekkarat, Steele James L.

Принадлежит:

An ethanologen for producing biofuel from one or more carbohydrates and reducing lactate and acetate production in a biofuel manufacturing process. The ethanologen is made by introducing into the ethanologen one or more exogenous genes required for production of a bacteriocin. The resulting ethanologen reduces lactate and acetate production by contaminant lactic acid bacteria by expression of the bacteriocin during the biofuel manufacturing process. Certain resulting ethanologens ferment sugars not naturally or not preferentially utilized by during the manufacturing process 122-. (canceled)23. An ethanologen comprising(a) one or more inactivated endogenous genes encoding a mannitol dehydrogenase;(b) one or more inactivated endogenous genes encoding a lactate dehydrogenase; and(c) one or more exogenous genes encoding a pyruvate decarboxylase and one or more exogenous genes encoding an alcohol dehydrogenase;wherein the ethanologen is an engineered lactic acid bacterium; andwhereby the engineered lactic acid bacterium produces more biofuel than a wild-type lactic acid bacterium having the same genetic background in a biofuel manufacturing process24. The ethanologen of claim 23 , wherein the one or more inactivated endogenous genes encoding a mannitol dehydrogenase comprises a deletion in a mannitol dehydrogenase 1 and/or a mannitol dehydrogenase 2 gene.25. The ethanologen of claim 23 , wherein the one or more inactivated endogenous genes encoding a lactate dehydrogenase comprises a deletion in a lactate dehydrogenase 1 (LDH1) claim 23 , lactate dehydrogenase 2 (LDH2) claim 23 , lactate dehydrogenase 3 (LDH3) claim 23 , and/or lactate dehydrogenase 4 (LDH4) gene.26. The ethanologen of claim 23 , wherein the ethanologen is capable of fermenting sugars not naturally or not preferentially fermented by a main fermenting microbe present in the biofuel manufacturing process.27Saccharomyces cerevisiae.. The ethanologen of claim 26 , wherein the main fermenting microbe ...

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Номер записи: 77

19-04-2018 дата публикации

GENETICALLY ENGINEERED BACTERIUM FOR THE PRODUCTION OF 3-HYDROXYBUTYRATE

Номер: US20180105847A1

Автор: Behrendorff James Bruce Yarnton Haycock, HILL Ryan Edward, Jensen Rasmus Overgaard, Juminaga Darmawi, Koepke Michael, Mueller Alexander Paul

Принадлежит:

The invention relates to a genetically engineered bacterium having an enzyme that converts acetyl-CoA to acetoacetyl-CoA, an enzyme that converts acetoacetyl-CoA to 3-hydroxybutyryl-CoA, and an enzyme that converts 3-hydroxybutyryl-CoA to 3-hydroxybutyrate. The bacterium may also have enzymes to produce other downstream products, such as 3-hydroxybutyryaldehyde, and 1,3-butanediol. Typically, the bacterium is capable of producing these products from a gaseous substrate, such as syngas or an industrial waste gas. 1. A genetically engineered C1-fixing bacterium comprising:(a) an enzyme that converts acetyl-CoA to acetoacetyl-CoA,(b) an enzyme that converts acetoacetyl-CoA to 3-hydroxybutyryl-CoA, and(c) an enzyme that converts 3-hydroxybutyryl-CoA to 3-hydroxybutyrate,wherein at least one of the enzymes is exogenous to the bacterium.2. The bacterium of claim 1 , wherein the enzyme that converts acetyl-CoA to acetoacetyl-CoA is thiolase (EC 2.3.1.9).3. The bacterium of claim 1 , wherein the enzyme that converts acetoacetyl-CoA to 3-hydroxybutyryl-CoA is 3-hydroxybutyryl-CoA dehydrogenase (EC 1.1.1.157) or acetoacetyl-CoA reductase (EC 4.2.1.36).4. The bacterium of claim 1 , wherein the enzyme that converts 3-hydroxybutyryl-CoA to 3-hydroxybutyrate is thioesterase (EC 3.1.2.20) claim 1 , phosphate butyryltransferase (EC 2.3.1.19) and butyrate kinase (EC 2.7.2.7) claim 1 , or CoA-transferase (EC 2.8.3.9).5. The bacterium of claim 1 , wherein the enzyme that converts 3-hydroxybutyryl-CoA to 3-hydroxybutyrate is stereospecific.6. The bacterium of claim 1 , wherein the 3-hydroxybutyrate is (R)-3-hydroxybutyrate claim 1 , (S)-3-hydroxybutyrate claim 1 , or a combination thereof.7. The bacterium of claim 1 , wherein the bacterium further comprises an isomerase that interconverts (R)-3-hydroxybutyrate and (S)-3-hydroxybutyrate.8. The bacterium of claim 1 , wherein the bacterium further comprises an enzyme that converts 3 -hydroxybutyrate to 3-hydroxybutyryaldehyde.9. The ...

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Номер записи: 78

20-04-2017 дата публикации

MICROORGANISM CAPABLE OF PRODUCING 1,4-BUTANEDIOL AND METHOD OF PRODUCING 1,4-BUTANEDIOL USING THE SAME

Номер: US20170107544A1

Автор: Cho Hwayoung, Cho Kwangmyung, Jung Yukyung, Park Jinhwan

Принадлежит:

A microorganism capable of producing 1,4-butanediol and a method of producing 1,4-butanediol using the same. 110.-. (canceled)11. A method of producing 1 ,4-BDO comprising culturing in the presence of succinate a genetically engineered microorganism and recovering 1 ,4-BDO from the culture , wherein the genetically engineered microorganism comprisesa genetic modification that inactivates or attenuates one or more genes encoding a polypeptide converting pyruvate to lactate,one or more genes encoding alcohol dehydrogenase,one or more genes encoding a polypeptide converting oxaloacetate to malate;a genetic modification that increases the expression of one or more genes encoding an enzyme that converts succinate to 4-hydroxybutyrate (4HB) in comparison to a parent microorganism not having the genetic modification; anda genetic modification that increases the expression of one or more genes encoding an enzyme that converts 4HB to 1,4-butanediol (1,4-BDO) in the genetically engineered microorganism in comparison to a parent microorganism not having the genetic modification.12. The method in claim 11 , wherein additional succinate is fed to the culture during the culturing.13. The method in claim 11 , wherein the culturing is performed at a dissolved oxygen concentration which is from about 1% to about 100% of a saturated concentration.14EscherichiaCorynebacterium. The method in claim 11 , wherein the microorganism belongs to genus claim 11 , or genus.15E. coli.. The method in claim 11 , wherein the microorganism is1619.-. (canceled)20. The method in claim 11 , wherein the activity of converting succinate to 4-HB is increased by introduction of one or more genes encoding a polypeptide converting succinate to succinyl-CoA claim 11 , one or more genes encoding a polypeptide converting succinyl-CoA to succinic semialdehyde (SSA) claim 11 , one or more genes encoding a polypeptide converting SSA to 4HB claim 11 , or a combination thereof in the microorganism.21. The method in ...

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Номер записи: 79

26-04-2018 дата публикации

ENGINEERING OF MULTI-CARBON SUBSTRATE UTILIZATION PATHWAYS IN METHANOTROPHIC BACTERIA

Номер: US20180112224A1

Автор: Leonard Effendi, Saville Renee M., Silverman Joshua A.

Принадлежит:

The present disclosure relates to genetically engineered methanotrophic bacteria with the capability of growing on a multi-carbon substrate (e.g., glucose) as a primary or sole carbon source and methods for growing methanotrophic bacteria on the multi-carbon substrate. 1. A recombinant methanotrophic bacterium , comprising at least one exogenous nucleic acid encoding a glucose utilization pathway component ,wherein glucose is not utilized as a carbon source by an unmodified parent methanotrophic bacterium;wherein the encoded glucose utilization pathway component comprises a glucose transporter and is expressed in a sufficient amount to permit growth of the recombinant methanotrophic bacterium on glucose as a primary carbon source; and{'i': Methylococcus capsulatus, Methylomonas', 'Methylosinus trichosporium, Methylosinus sporium, Methylocystis parvus, Methylomonas methanica, Methylomonas albus, Methylobacter capsulatus, Methylomonas flagellata, Methylacidiphilum infernorum, Methylomicrobium alcaliphilum, Methylocella silvestris, Methylocella palustris, Methylocella tundrae, Methylocystis daltona, Methylocystis bryophila,', 'Methylocapsa aurea., 'wherein the methanotrophic bacterium is selected from sp. 16A, or'}2Escherichia coli, Bacillus subtilis, Corynebacterium glutamicum, Saccharomyces cerevisiae, Zymomonas mobilis; Agrobacterium tumefaciens, Sinorhizobium meliloti; Rhodobacter sphaeroides; Paracoccus versutus; Pseudomonas fluorescens, Pseudomonas putida, Salmonella enterica, Escherichia fergusonii, Salmonella enteric, Yersinia pestis, Yersinia pseudotuberculosis, Yersinia enterocolitica, Shigella flexneri, Shigella sonnei, Shigella boydii, Shigella dysenteriae, Pectobacterium atrosepticum, Pectobacterium wasabiae, Erwinia tasmaniensis, Erwinia pyrifoliae, Erwinia amylovora, Erwinia billingiae, Buchnera aphidicola, EnterobacterEnterobacter cloacae, Enterobacter asburiae, Enterobacter aerogenes, Cronobacter sakazakii, Cronobacter turicensis, Klebsiella pneumoniae ...

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Номер записи: 80

26-04-2018 дата публикации

CYANOBACTERIAL STRAINS CAPABLE OF UTILIZING PHOSPHITE

Номер: US20180112225A1

Автор: Budinoff Charles Ryan, Deng Ming-De, Enke Heike, JOCHEM Frank, ROESSLER PAUL GORDON, WANG Kui, Weissert Christian, Zhu Songhua

Принадлежит: Algenol Biotech LLC

The invention provides genetically modified cyanobacterial cells that are capable of utilizing phosphite as a primary phosphorus source, and can out-compete contaminant organisms for certain forms of phosphorus more effectively. 1. A genetically modified cyanobacterial cell for the production of a product of interest , comprising:a) at least one recombinant gene that encodes a heterologous phosphite dehydrogenase enzyme EC:1.20.1.1 that catalyzes the oxidation of phosphite to phosphate;b) an operon comprising at least one recombinant phosphite transporter gene encoding at least one phosphite transporter protein for transporting phosphite into the cell; andc) at least one recombinant production gene encoding a polypeptide for the production of said product of interest.2CyanotheceRalstonia. The genetically modified cyanobacterial cell of claim 1 , wherein the recombinant phosphite dehydrogenase gene encodes a polypeptide that has a sequence identity of greater than 60% to the protein sequence of the phosphite dehydrogenase enzyme from (SEQ ID NO: 16) or from (SEQ ID NO: 12).3. The genetically modified cyanobacterial cell of claim 1 , wherein the recombinant phosphite dehydrogenase gene is operably linked to a regulatable promoter selected from the group consisting of: a metal-regulatable promoter claim 1 , a nitrate-regulatable promoter claim 1 , and a phosphorus-regulatable promoter.4. The genetically modified cyanobacterial cell of claim 1 , wherein at least one of the phosphite transporter genes is derived from a different organism than the phosphite dehydrogenase gene.5Desulfotignum phosphitoxidansCyanothece.. The genetically modified cyanobacterial cell of claim 1 , wherein the at least one phosphite transporter gene is from or6. The genetically modified cyanobacterial cell of claim 1 , wherein the at least one phosphite transporter protein is selected from the group consisting of PtxA claim 1 , PtxB claim 1 , PtxC claim 1 , and PtdC.7. The genetically modified ...

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Номер записи: 81

09-04-2020 дата публикации

TRANSGENIC YEAST AND METHOD FOR PRODUCING ETHANOL USING THE SAME

Номер: US20200109373A1

Автор: Onishi Toru

Принадлежит: TOYOTA JIDOSHA KABUSHIKI KAISHA

The present disclosure is intended to reduce the amount of glycerin produced as a byproduct in ethanol fermentation to a significant extent using a transgenic yeast comprising a gene having the pentose assimilating ability and encoding glycerin dehydrogenase having a mitochondrial transport signal introduced thereinto. 1. A transgenic yeast having pentose assimilating ability , comprising a gene encoding glycerin dehydrogenase having a mitochondrial transport signal introduced thereinto.2. The transgenic yeast according to claim 1 , wherein the glycerin dehydrogenase is NAD-dependent glycerin dehydrogenase having activity of converting NAD into NADPH.3. The transgenic yeast according to claim 1 , wherein the gene encoding glycerin dehydrogenase encodes the protein (a) or (b):(a) a protein comprising the amino acid sequence as shown in SEQ ID NO: 2; or(b) a protein comprising an amino acid sequence having 70% or higher sequence identity to the amino acid sequence as shown in SEQ ID NO: 2, having mitochondrial locality, and having activity of generating dihydroxyacetone using glycerin as a substrate.4. The transgenic yeast according to claim 1 , wherein the gene encoding glycerin dehydrogenase encodes a fusion protein comprising a mitochondrial transport signal and the protein (a) or (b):(a) a protein comprising the amino acid sequence as shown in SEQ ID NO: 4; or(b) a protein comprising an amino acid sequence having 70% or higher sequence identity to the amino acid sequence as shown in SEQ ID NO: 4, and having activity of generating dihydroxyacetone using glycerin as a substrate.5. The transgenic yeast according to claim 1 , wherein the pentose is xylose and/or arabinose.6. The transgenic yeast according to claim 1 , which comprises the xylose isomerase gene introduced thereinto and has xylose assimilating ability.7. The transgenic yeast according to claim 6 , which further comprises a xylulokinase gene introduced thereinto.8. The transgenic yeast according to claim ...

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Номер записи: 82

07-05-2015 дата публикации

MICROORGANISM WITH INCREASED IRON-REGULATED ABC TRANSPORTER ACTIVITY AND METHOD OF PRODUCING HYDROXYCARBOXYLIC ACID BY USING THE MICROORGANISM

Номер: US20150125919A1

Автор: Cho Hwayoung, Cho Kwangmyung, Park Jinhwan, Rhee Hongsoon

Принадлежит:

A recombinant microorganism having increased iron-regulated ABC transporter activity and increased hydroxycarboxylic acid production, as well as a method of producing a hydroxycarboxylic acid using the recombinant microorganism, and a method of producing the recombinant microorganism. 1. A recombinant microorganism having an increased iron-regulated ABC transporter activity in comparison with a parent cell of the microorganism , and producing an increased amount of hydroxycarboxylic acid as compared to a parent cell of the microorganism.2. The recombinant microorganism of claim 1 , wherein the transporter is (a) SufB claim 1 , (b) SufD claim 1 , (c) ABC-type cobalamin/Fe-siderophore transport system claim 1 , periplasmic component claim 1 , or (d) a combination thereof.3. The recombinant microorganism of claim 1 , whereinthe SufB comprises an amino acid sequence having a sequence identity of 95% or more to SEQ ID NO: 1,the SufD comprises an amino acid sequence having a sequence identity of 95% or more to SEQ ID NO: 3, and{'sup': '3+', 'the ABC-type cobalamin/Fe-siderophore transport system, periplasmic component comprises an amino acid sequence having a sequence identity of 95% or more to SEQ ID NO: 5.'}4. The recombinant microorganism of claim 1 , wherein the recombinant microorganism has a genetic modification that increases the activity of the transporter as compared to an activity of the parent cell of the microorganism.5. The recombinant microorganism of claim 1 , wherein the recombinant microorganism comprises an exogenous gene that encodes the transporter.6. The recombinant microorganism of claim 5 , wherein the exogenous gene encodes an amino acid sequence having a sequence identity of 95% or more to SEQ ID NOS: 1 claim 5 , 3 claim 5 , or 5.7. The recombinant microorganism of claim 1 , wherein the hydroxycarboxylic acid has a chemical structure represented by Formula 1 below:{'br': None, 'sup': '1', 'HO—R—COOH\u2003\u2003[Formula 1]'}{'sup': '1', 'sub': 1', ...

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Номер записи: 83

24-07-2014 дата публикации

Recombinant microorganisms and methods of use thereof

Номер: US20140206901A1

Автор: Michael Koepke, Shilpa NAGARAJU, Wendy Yiting Chen

Принадлежит: Lanzatech New Zealand Ltd

The invention relates to methods for the production of chemical compounds, particularly but not exclusively ethanol, by microbial fermentation. Also described are genetically modified micro-organisms capable of using carbon monoxide to produce one or more products, particularly but not exclusively ethanol as a main product, and producing a reduced amount or substantially no 2,3-butanediol and/or a precursor thereof.

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Номер записи: 84

27-05-2021 дата публикации

ACETATE TOXICITY TOLERANCE IN RECOMBINANT MICROBIAL HOST CELLS

Номер: US20210155944A1

Автор: Covalla Sean, Froehlich Allan, Henningsen Brooks, Zelle Rintze

Принадлежит:

Acetate is a potent microbial inhibitor which can affect the performance of yeast in ethanolic fermentation. The present disclosure provides a recombinant microbial host cell having (i) a first genetic modification for increasing the activity of one or more proteins that function in a first metabolic pathway to convert acetate into an alcohol in the microbial host cell; (ii) a second genetic modification for increasing the activity of one or more proteins that function in a second metabolic pathway to import glycerol in the recombinant microbial host cell (iii) a third genetic modification for increasing the activity of one or more proteins that function in a third metabolic pathway to convert a C5 carbohydrate into ethanol in the microbial host cell. The recombinant microbial host cell comprises and natively expresses native proteins that function in a fourth native metabolic pathway to produce glycerol in the microbial host cell. 1. A recombinant microbial host cell having:a first genetic modification for increasing the activity of one or more proteins that function in a first metabolic pathway to convert acetate into an alcohol in the microbial host cell;a second genetic modification for increasing the activity of one or more proteins that function in a second metabolic pathway to import glycerol in the recombinant microbial host cell; anda third genetic modification for increasing the activity of one or more proteins that function in a third metabolic pathway to convert a C5 carbohydrate into the alcohol in the microbial host cell;wherein the recombinant microbial host cell comprises and natively expresses native proteins that function in a fourth native metabolic pathway to produce glycerol in the microbial host cell.2. The recombinant microbial host cell of claim 1 , wherein the alcohol is ethanol.3. The recombinant microbial host cell of claim 1 , wherein the one or more proteins that function in the first claim 1 , second or third metabolic pathway are ...

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Номер записи: 85

12-05-2016 дата публикации

Integration of a Polynucleotide Encoding a Polypeptide that Catalyzes Pyruvate to Acetolactate Conversion

Номер: US20160130612A1

Автор: ANTHONY LARRY CAMERON, Maggio-Hall Lori Ann

Принадлежит:

The invention relates to recombinant host cells having at least one integrated polynucleotide encoding a polypeptide that catalyzes a step in a pyruvate-utilizing biosynthetic pathway, e.g., pyruvate to acetolactate conversion. The invention also relates to methods of increasing the biosynthetic production of isobutanol, 2,3-butanediol, 2-butanol or 2-butanone using such host cells. 147-. (canceled)48. A recombinant host cell comprising:{'i': Bacillus subtilis, Klebsiella pneumonia, Lactococcus lactis, Staphylococcus aureus, Listeria monocytogenes, Streptococcus mutans, Streptococcus thermophiles, Vibrio angustum', 'Bacillus cereus., '(a) a polynucleotide encoding a polypeptide which catalyzes the substrate to product conversion of pyruvate to acetolactate wherein the polypeptide is an acetolactate synthase from , or'}{'i': Lactococcus lactis, Vibrio cholera, Pseudomonas aeruginosa, Pseudomonas fluorescens', 'Anaerostipes caccae., '(b) a polynucleotide encoding a polypeptide which catalyzes the substrate to product conversion of acetolactate to 2,3-dihydroxyisovalerate wherein the polypeptide is a ketol-acid reductoisomerase from , or'}{'i': Escherichia coli, Bacillus subtilis, Methanococcus maripaludis', 'Streptococcus mutans, '(c) a polynucleotide encoding a polypeptide which catalyzes the substrate to product conversion of 2,3-dihydroxyisovalerate to α-ketoisovalerate wherein the polypeptide is a dihydroxyacid dehydratase from , or ; and'}{'i': Listeria grayi, Lactococcus lactis', 'Macrococcus caseolyticus., '(d) a polynucleotide encoding a polypeptide which catalyzes the substrate to product conversion of α-ketoisovalerate to isobutyraldehyde wherein the polypeptide is a branched-chain α-keto acid decarboxylase from , or'}49Achromobacter xylosoxidansBeijerinkia indica.. The recombinant host cell of further comprising a polynucleotide encoding a polypeptide which catalyzes the substrate to product conversion isobutyraldehyde to isobutanol wherein the polypeptide ...

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Номер записи: 86

10-05-2018 дата публикации

METHODS FOR THE POSITIVE SELECTION OF ETHANOL OVERPRODUCING MUTANTS FROM SACCHAROMYCES CEREVISIAE

Номер: US20180127708A1

Автор: ABBAS Charles A, DMYTRUK Kostyantyn, KSHANOVSKA Barbara, Sibirny Andriy

Принадлежит:

Described herein are new approaches for the selection of strains with increased ethanol production from hydrolyzed starch derived sugars. An industrial production strain of AS400 was subjected to positive selection of mutants resistant to toxic concentrations of oxythiamine, trehalose, 3-bromopyruvate, glyoxylic acid, and glucosamine. The selected mutants are characterized by 5-8% increase in ethanol yield (g gof consumed glucose) as compared to the parental industrial ethanol-producing strain. A multiple-step selection approach that consisted of the sequential selection using glyoxylic acid, glucosamine and bromopyruvate as selective agents resulted in a 12% increase in ethanol yield during fermentation on industrial media. These results indicate that the selection methods provided herein are useful for producing a variety of strains that are promising candidates for industrial ethanol production. 1S. cerevisiae. A method of making a strain with enhanced ethanol producing characteristics comprising ,{'i': 'S. cerevisiae', 'contacting a culture of a parent strain of with a sufficient amount of a first selection agent selected from the group consisting of oxythiamine, trehalose, bromopyruvate, glycoxylic acid and glucosamine for the selection agent to be toxic to the parent strain;'}growing the contacted culture on a plating medium containing a sugar as a carbon source;selecting candidate spontaneous mutant strains of the culture that grow on the plating medium, and{'i': 'S. cerevisiae', 'measuring an ethanol producing characteristic of the candidate spontaneous mutant strains to determine whether the candidate strain demonstrates enhanced ethanol production characteristics in comparison to the parent strain and if so, obtaining the strain with enhanced ethanol producing characteristics.'}2. The method of wherein the obtained spontaneous mutant strain with enhanced ethanol production characteristics is contacted with an amount of a second selection agent selected ...

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Номер записи: 87

11-05-2017 дата публикации

PRODUCTION OF XYLITOL FROM GLUCOSE BY A RECOMBINANT STRAIN

Номер: US20170130209A1

Автор: CHANG YIMING, DEFRETIN SOPHIE, DIEFENBACHER MELANIE, GERARD TANIA, HEYSEN ARNAUD, HONDA MALCA SUMIRE, SCHAEFER ASTRID, Schwab Markus, THOR FRIEDERIKE

Принадлежит:

The present invention relates to a recombinant microbial host for the production of xylitol, the recombinant microbial host containing a nucleic acid sequence encoding an NAD+-specific D-arabitol 4-oxidoreductase (EC 1.1.1.11) using D-arabitol as substrate and producing D-xylulose as product, and a nucleic acid sequence encoding an NADPH-specific xylitol dehydrogenase using D-xylulose as substrate and producing xylitol as product. 117-. (canceled)18. A recombinant host cell capable of producing xylitol , wherein said host cell comprises:{'sup': '+', 'an heterologous nucleic acid sequence encoding a NAD-specific D-arabitol 4-oxidoreductase (EC 1.1.1.11) using D-arabitol as substrate and producing D-xylulose as product; and,'}an heterologous nucleic acid sequence encoding a NADPH-specific xylitol dehydrogenase using D-xylulose as substrate and producing xylitol as product.19. The recombinant host cell according to claim 18 , wherein the host cell produces D-arabitol from D-glucose claim 18 , in particular under high osmotic pressure medium.20. The recombinant host cell according to claim 19 , wherein the host cell does not consume D-arabitol as a sole carbon source.21. The recombinant host cell according claim 18 , wherein the host cell is selected from bacteria claim 18 , fungi and yeast.22. The recombinant host cell according to claim 21 , wherein the host cell is an osmophilic or osmotolerant yeast.23Pichia ohmeri.. The recombinant host cell according to claim 21 , wherein the host cell is24E. coliRalstonia solanacearum.. The recombinant host cell according to claim 18 , wherein the NAD-specific D-arabitol 4-oxidoreductase (EC 1.1.1.11) is from and/or25. The recombinant host cell according to claim 24 , wherein the NAD-specific D-arabitol 4-oxidoreductase (EC 1.1.1.11) comprises a sequence selected from the group of SEQ ID NO: 2 claim 24 , 43 and sequences with 1-3 additions claim 24 , substitutions or deletions of amino acids.26. The recombinant host cell ...

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Номер записи: 88

02-05-2019 дата публикации

FERMENTATIVE GLYCEROL-FREE ETHANOL PRODUCTION

Номер: US20190127745A1

Автор: GUADALUPE MEDINA Victor Gabriel, Pronk Jacobus Thomas, VAN MARIS Antonius Jeroen Adriaan

Принадлежит:

The present invention relates to a yeast cell, in particular a recombinant yeast cell, the cell lacking enzymatic activity needed for the NADH-dependent glycerol synthesis or the cell having a reduced enzymatic activity with respect to the NADH-dependent glycerol synthesis compared to its corresponding wild-type yeast cell, the cell comprising one or more heterologous nucleic acid sequences encoding an NAD-dependent acetylating acetaldehyde dehydrogenase (EC 1.2.1.10) activity. The invention further relates to the use of a cell according to the invention in the preparation of ethanol. 1. Transgenic yeast cells comprising one or more recombinant heterologous , nucleic acid sequences encoding a protein with NAD+-dependent acetylating acetaldehyde dehydrogenase activity (EC 1.2.1.10) ,wherein said cells lack enzymatic activity needed for the NADH-dependent glycerol synthesis, orhave a reduced enzymatic activity with respect to the NADH-dependent glycerol synthesiscompared to a corresponding wild-type yeast cell, andwherein said lack of enzymatic activity or reduced enzymatic activity is the result of a genomic mutation in said cells of at least one gene selected from the group consisting of GPD1, GPD2, GPP1 and GPP2,wherein at least one said NAD+-dependent acetylating acetaldehyde dehydrogenases comprises a sequence that has at least 90% identity to SEQ ID NO:2 or SEQ ID NO:29, andwherein said cells further comprise one or more nucleic acid sequences encoding NAD+-dependent alcohol dehydrogenase activity (EC 1.1.1.1).2. The transgenic yeast cells of claim 1 , wherein the one or more recombinant heterologous claim 1 , nucleic acid sequences encoding a protein with NAD+-dependent acetylating acetaldehyde dehydrogenase activity (EC 1.2.1.10) comprises a bifunctional protein that catalyzes the reversible conversion of acetyl-Coenzyme A to acetaldehyde and the subsequent reversible conversion of acetaldehyde to ethanol.3. The transgenic yeast cells of claim 2 , wherein the ...

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Номер записи: 89

19-05-2016 дата публикации

OXYGEN-TOLERANT CoA-ACETYLATING ALDEHYDE DEHYDROGENASE CONTAINING PATHWAY FOR BIOFUEL PRODUCTION

Номер: US20160138049A1

Автор: Lan Ethan I., Liao James C.

Принадлежит:

Provided herein are metabolically-modified microorganisms useful for producing biofuels. More specifically, provided herein are methods of producing high alcohols including isobutanol, 1-butanol, 1-propanol, 2-methyl-1-butanol, 3-methyl-1-butanol and 2-phenylethanol from a suitable substrate. 1. A recombinant microorganism the converts a carbon source to a biofuel alcohol comprising a recombinantly expressed oxygen tolerant CoA-acylating aldehyde dehydrogenase (PduP) comprising a sequence that is at least 85% identical to SEQ ID NO:34.2. The recombinant microorganism of claim 1 , wherein the microorganism is a photoautotroph or photoheterotroph microorganism.3. The recombinant microorganism of claim 2 , wherein the microorganism produces a metabolite in the production of n-butanol and/or producing n-butanol wherein the metabolite and/or n-butanol is produced through a malonyl-CoA dependent pathway comprising an oxygen tolerant CoA-acylating aldehyde dehydrogenase.4. The recombinant photoautotroph or photoheterotroph microorganism of claim 2 , wherein the organism comprises expression or elevated expression of an enzyme that converts acetyl-CoA to malonyl-CoA claim 2 , malonyl-CoA to Acetoacetyl-CoA claim 2 , and at least one enzyme that converts acetoacetyl-CoA to (R)- or (S)-3-hydroxybutyryl-CoA claim 2 , (R)- or (S)-3-hydroxybutyryl-CoA to crotonyl-CoA claim 2 , crotonyl-CoA to butyryl-CoA claim 2 , butyryl-CoA to butyraldehyde and butyraldehyde to 1-butanol.5. The recombinant microorganism of claim 3 , wherein the microorganism comprises a metabolic pathway for the production of 1-butanol that is an NADPH dependent pathway.6. The recombinant microorganism of claim 3 , wherein the photoautotrophic or photoheterotrophic microorganism is engineered to express or overexpress one or more polypeptides that convert acetyl-CoA to malonyl-CoA and malonyl-CoA to Acetoacetyl-CoA.7. The recombinant microorganism of claim 6 , wherein the one or more polypeptides comprises a ...

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Номер записи: 90

18-05-2017 дата публикации

Genetic engineered bacteria and methods for promoting production of succinic acid or lactic acid

Номер: US20170137829A1

Автор: Guang-Way Jang, Hsi-Yen Hsu, Hsiang-Yuan Chu, Jhong-De Lin, Pei-Ching Chang, Ya-Lin Lin

Принадлежит: Industrial Technology Research Institute ITRI

A genetic engineered bacteria without or comprising a plurality of important metabolic enzyme related genes is provided. When the by-product or waste of fruit and vegetable is used as the culture medium, a large quantity of succinic acid or lactic acid can be produced via fermentation. A method of producing succinic acid and lactic acid using the genetic engineered bacteria is also provided.

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Номер записи: 91

09-05-2019 дата публикации

Genetically Engineered Strain

Номер: US20190136269A1

Автор: BAI Yajun, CAI Yujie, DING Yanrui, LIU Jinbin, XIONG Tianzhen, ZHENG Xiaohui

Принадлежит:

The present disclosure discloses a genetically engineered strain, belonging to the technical field of bioengineering. L-amino acid oxidase genes, α-keto acid decarboxylase genes, alcohol dehydrogenase genes, and enzyme genes capable of reducing NAD(P) to NAD(P)H are introduced into the genetically engineered strain of the present disclosure. The present disclosure further discloses a construction method and application of a recombinant genetically engineered strain. When being applied to the biosynthesis of phenylethanoids, the method of the present disclosure has the characteristics of simple operation, low cost, and high synthesis efficiency and optical purity of the product, and has good industrialization prospects. 1Escherichia coli. A recombinant strain , which is capable of producing phenylethanoids at low cost , and capable of simultaneously expressing four enzymes which are L-amino acid oxidase , α-keto acid decarboxylase , alcohol dehydrogenase , and an enzyme capable of reducing NAD(P) to NAD(P)H.2Escherichia coliProteus mirabilisCosenzaea myxofaciens. The recombinant strain according to claim 1 , characterized in that the L-amino acid oxidase is from ATCC 29906 or ATCC 19692.3Escherichia coliProteus mirabilisLactococcus lactis. The recombinant strain according to claim 1 , characterized in that the α-keto acid decarboxylase is from ATCC 29906 or ATCC 19435.4Escherichia coliEscherichia coli. The recombinant strain according to claim 1 , characterized in that the alcohol dehydrogenase is from BL21(DE3).5Escherichia coli. The recombinant strain according to claim 1 , characterized in that the enzyme capable of reducing NAD(P) to NAD(P)H is formate dehydrogenase claim 1 , glucose dehydrogenase or phosphite dehydrogenase.6Escherichia coliKomagataella phaffiiBacillus subtilisPseudomonas abietaniphila. The recombinant strain according to claim 5 , characterized in that the enzyme capable of reducing NAD(P) to NAD(P)H is formate dehydrogenase from ATCC 76273 ...

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Номер записи: 92

26-05-2016 дата публикации

Ethanol Production in Microorganisms

Номер: US20160145649A1

Автор: Green Brian D., Reppas Nikos Basil, Robertson Dan Eric

Принадлежит:

The present disclosure relates to methods and compositions for engineering photoautotrophic organisms to convert carbon dioxide and light into fatty acid esters and other molecules, including biofuels. The molecules are then secreted by the organism into a growth medium. 1. A method for production of ethanol , comprising:culturing an engineered photosynthetic microbe in a culture medium in the presence of light and inorganic carbon, wherein the engineered photosynthetic microbe comprises a recombinant pyruvate decarboxylase nucleic acid sequence encoding a pyruvate decarboxylase having EC 4.1.1.1 and a recombinant alcohol dehydrogenase nucleic acid sequence encoding an alcohol dehydrogenase having EC 1.1.1.1 or EC 1.1.1.2, wherein the copy number of at least the alcohol dehydrogenase nucleic acid sequence in the engineered photosynthetic microbe is greater than the copy number of an alcohol dehydrogenase nucleic acid sequence in a control engineered photosynthetic microbe comprising a single recombinant alcohol dehydrogenase gene and a single recombinant pyruvate decarboxylase gene regulated together by a single promoter, and wherein the engineered photosynthetic microbe produces ethanol in an amount greater than a non-engineered photosynthetic microbe, when cultured under identical conditions.2. The method of claim 1 , wherein different promoters are used to achieve differential expression of each enzyme.3. The method of claim 2 , wherein one of the recombinant nucleic acid sequences is modulated by an inducible promoter and one of the recombinant nucleic acid sequences is modulated by a constitutive promoter.4. The method of claim 3 , wherein the copy number of the recombinant alcohol dehydrogenase nucleic acid sequence is modulated by a constitutive promoter.5. The method of claim 3 , wherein the copy number of the recombinant pyruvate decarboxylase nucleic acid sequence is modulated by a chemically-affected promoter.6. The method of claim 5 , wherein the copy ...

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Номер записи: 93

10-06-2021 дата публикации

Biological Production of Multi-Carbon Compounds from Methane

Номер: US20210171988A1

Автор: Coleman William J., COTTAREL Guillaume, Groban Eli S., JAVAN Akbar F., KAMIMURA Roy, MULEY Sheela, SUN Jianping, VIDANES Genevieve M.

Принадлежит:

Multi-carbon compounds such as ethanol, n-butanol, sec-butanol, isobutanol, tert-butanol, fatty (or aliphatic long chain) alcohols, fatty acid methyl esters, 2,3-butanediol and the like, are important industrial commodity chemicals with a variety of applications. The present invention provides metabolically engineered host microorganisms which metabolize methane (CH) as their sole carbon source to produce multi-carbon compounds for use in fuels (e.g., bio-fuel, bio-diesel) and bio-based chemicals. Furthermore, use of the metabolically engineered host microorganisms of the invention (which utilize methane as the sole carbon source) mitigate current industry practices and methods of producing multi-carbon compounds from petroleum or petroleum-derived feedstocks, and ameliorate much of the ongoing depletion of arable food source “farmland” currently being diverted to grow bio-fuel feedstocks, and as such, improve the environmental footprint of future bio-fuel, bio-diesel and bio-based chemical compositions. 191-. (canceled)92. A genetically modified methanotroph comprising a heterologous polynucleotide encoding for an alcohol dehydrogenase (ADH) , wherein the alcohol dehydrogenase can catalyze the conversion of isobutyraldehyde to isobutanol and comprises an amino acid sequence having at least 90% sequence homology to SEQ ID NO: 10 , and wherein said methanotroph is capable of converting formaldehyde to pyruvate through a type I RuMP pathway or a type II serine pathway.93. The methanotroph of claim 92 , wherein the methanotroph further comprises a heterologous polynucleotide encoding a ketoacid decarboxylase (KDC) claim 92 , wherein the ketoacid decarboxylase can catalyze the conversion of ketoisovalerate to isobutryaldehyde.94. The methanotroph of claim 93 , wherein the methanotroph further comprises a heterologous polynucleotide encoding an acetolactate synthase (ALS) claim 93 , a heterologous polynucleotide encoding a ketol-acid reductoisomerase (KARI) claim 93 , ...

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Номер записи: 94

24-05-2018 дата публикации

A METHOD OF PRODUCING ALCOHOLS

Номер: US20180142266A1

Автор: BÜLTER Thomas, Gilch Stefan, Haas Thomas, Hecker Anja

Принадлежит:

There is provided a microbial cell which is capable of producing at least one higher alcohol, wherein the microbial cell is genetically modified to include an increased expression relative to its wild type cell of at least one acyl-CoA reductase (E). In particular, there is provided a microbial cell capable of producing at least one higher alcohol, wherein the microbial cell is genetically modified to include an increased expression relative to its wild type cell of at least one acyl-CoA reductase (E) and wherein the cell is capable of producing a carboxylic acid and/or ester thereof using ethanol-carboxylate fermentation. 1. A microbial cell capable of producing at least one higher alcohol , wherein the microbial cell is genetically modified to comprise an increased expression relative to its wild type cell of at least one acyl-CoA reductase (E) and wherein the microbial cell is capable of producing a carboxylic acid and/or ester thereof using ethanol-carboxylate fermentation.2. The microbial cell according to claim 1 , wherein the microbial cell expresses at least one enzyme selected from the group consisting of alcohol dehydrogenase E claim 1 , acetaldehyde dehydrogenase E claim 1 , acetoacetyl-CoA thiolase E claim 1 , 3-hydroxybutyryl-CoA dehydrogenase E claim 1 , 3-hydroxybutyryl-CoA dehydratase E claim 1 , butyryl-CoA dehydrogenase E claim 1 , electron transfer flavoprotein subunit E claim 1 , coenzyme A transferase E claim 1 , acetate kinase E claim 1 , phosphotransacetylase E.3Clostridium kluyveriC. carboxidivorans.. The microbial cell according claim 1 , wherein the microbial cell is from a microorganism selected from the group consisting of and4. The microbial cell according to claim 1 , wherein acyl-CoA reductase (E) is capable of catalysing Reaction 1 and/or Reaction 2(a) below:{'br': None, 'Acyl-CoA+2NAD(P)H→Fatty Alcohol\u2003\u2003Reaction 1{'br': None, 'sup': '+', 'Butyryl-CoA+NAD(P)H-->Butanal+CoA+NAD(P).\u2003\u2003Reaction 2(a)5Clostridium ...

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Номер записи: 95

24-05-2018 дата публикации

ELECTRON CONSUMING ETHANOL PRODUCTION PATHWAY TO DISPLACE GLYCEROL FORMATION IN S. CEREVISIAE

Номер: US20180142267A1

Автор: Argyros Aaron, Barrett Trisha, IV Arthur J., Shaw

Принадлежит:

The present invention provides for a mechanism to completely replace the electron accepting function of glycerol formation with an alternative pathway to ethanol formation, thereby reducing glycerol production and increasing ethanol production. In some embodiments, the invention provides for a recombinant microorganism comprising a down-regulation in one or more native enzymes in the glycerol-production pathway. In some embodiments, the invention provides for a recombinant microorganism comprising an up-regulation in one or more enzymes in the ethanol-production pathway. 160-. (canceled)61. A co-culture comprising at least two host cells wherein (i) a heterologous nucleic acid encoding a phosphoketolase;', '(ii) at least one heterologous nucleic acid encoding an enzyme in an acetyl-CoA production pathway;', '(iii) a heterologous nucleic acid encoding a bifunctional acetaldehyde-alcohol dehydrogenase; and,', '(iv) at least one genetic modification that leads to the down-regulation of an enzyme in a glycerol-production pathway; and,, '(a) one of the host cells comprises(b) another host cell that is genetically distinct from (a).62. The co-culture of claim 61 , wherein the host cell is a yeast and the genetically distinct host cell is a yeast or bacterium.63. The recombinant microorganism of claim 61 , wherein said phosphoketolase is a single-specificity phosphoketolase with the Enzyme Commission Number 4.1.2.9.64. The recombinant microorganism of claim 61 , wherein said phosphoketolase is dual-specificity phosphoketolase with the Enzyme Commission Number 4.1.2.22.65Aspergillus, Neurospora, Lactobacillus, Bifidobacterium, Penicillium, LeuconostocOenococcus.. The recombinant microorganism of claim 61 , wherein said phosphoketolase is from a genus selected from the group consisting of claim 61 , and66. The recombinant microorganism of claim 61 , wherein said phosphoketolase corresponds to a polypeptide selected from a group consisting of SEQ ID NOs: 9 claim 61 , 11 claim ...

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Номер записи: 96

24-05-2018 дата публикации

ITERATIVE PLATFORM FOR THE SYNTHESIS OF ALPHA FUNCTIONALIZED PRODUCTS

Номер: US20180142273A1

Автор: CHEONG Seokjung, CLOMBURG James M., GONZALEZ Ramon

Принадлежит:

The use of microorganisms to make alpha-functionalized chemicals and fuels, (e.g. alpha-functionalized carboxylic acids, alcohols, hydrocarbons, amines, and their beta-, and omega-functionalized derivatives), by utilizing an iterative carbon chain elongation pathway that uses functionalized extender units. The core enzymes in the pathway include thiolase, dehydrogenase, dehydratase and reductase. Native or engineered thiolases catalyze the condensation of either unsubstituted or functionalized acyl-CoA primers with an alpha-functionalized acetyl-CoA as the extender unit to generate alpha-functionalized β-keto acyl-CoA. Dehydrogenase converts alpha-functionalized β-keto acyl-CoA to alpha-functionalized β-hydroxy acyl-CoA. Dehydratase converts alpha-functionalized β-hydroxy acyl-CoA to alpha-functionalized enoyl-CoA. Reductase converts alpha-functionalized enoyl-CoA to alpha-functionalized acyl-CoA. The platform can be operated in an iterative manner (i.e. multiple turns) by using the resulting alpha-functionalized acyl-CoA as primer and the aforementioned alpha-functionalized extender unit in subsequent turns of the cycle. Termination pathways acting on any of the four alpha-functionalized CoA thioester intermediates terminate the platform and generate various alpha-functionalized carboxylic acids, alcohols and amines with different β-reduction degree. 136-) (cancel)37) A genetically engineered microorganism comprising means for: i) an acyl-CoA synthase which converts the alpha-functionalized CoA thioester extender unit from an alpha-functionalized acid;', 'ii) an acyl-CoA transferase which converts the alpha-functionalized CoA thioester extender unit from said alpha-functionalized acid;', 'iii) a phosphotransacylase and a carboxylate kinase which converts the alpha-functionalized CoA thioester extender unit from said alpha-functionalized acid; or', 'iv) other one or more enzyme(s) that allows production of said alpha-functionalized CoA thioester extender unit from a ...

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Номер записи: 97

25-05-2017 дата публикации

NEW MICROORGANISM AND METHOD FOR THE PRODUCTION OF 1.2-PROPANEDIOL BASED ON NADPH DEPENDENT ACETOL REDUCTASE AND IMPROVED NADPH SUPPLY

Номер: US20170145446A1

Автор: ALIPRANDI Pascale, BESTEL CORRE Gwénaëlle, NAVARRO Emilie, Raynaud Céline, Soucaille Philippe

Принадлежит: METABOLIC EXPLORER

The present invention relates to a recombinant microorganism useful for the production of 1,2-propanediol and process for the preparation of 1,2-propanediol. The microorganism of the invention is modified in a way that the 1,2-propanediol production is improved by enhancing NADPH dependent HAR activity. 2. The method of claim 1 , wherein the NADPH availability is increased in the microorganism by at least one of the following genetic modifications:the pntAB gene operon coding for a nicotinamide nucleotide transhydrogenase is overexpressed, and/orthe pgi gene coding for a phosphoglucose isomerase is attenuated, and/orthe pfkA gene coding for a phosphofructokinase is attenuated, and/orthe zwf gene coding for a glucose-6-phosphate dehydrogenase is overexpressed, and/orthe yjeF gene coding for an ADP-dependent dehydratase is overexpressed, and/orthe gapN gene coding for a NADP-dependent glyceraldehyde-3-phosphate dehydrogenase is overexpressed, and/ora mutant lpd*gene coding for a NADP-dependent lipoamide dehydrogenase is overexpressed.3. The method of or claim 1 , wherein the gldA gene coding for a NADH dependent glycerol dehydrogenase is deleted in the microorganism.4. The method of any one of to claim 1 , wherein the microorganism further comprises the deletion of the yqhD gene coding for a methylglyoxal reductase.5Enterobacteriaceae, Bacillaceae, Clostridiaceae, Streptomycetaceae. The method of any one of to claim 1 , wherein the microorganism is selected among the group consisting in and yeasts.6Escherichia coli, Klebsiella pneumoniae, Thermoanaerobacterium thermosaccharolyticum, Clostridium sphenoidesSaccharomyces cerevisiae.. The method of claim 5 , wherein the microorganism is selected among the group consisting of and7E. coli.. The method of claim 6 , wherein the microorganism is8Clostridium beijerinckiiThermoanaerobacter brockiiEntamoeba histolyticaGluconobacter oxydansHypocrea jecorinaBacillus subtilis.. A microorganism genetically modified for the production ...

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Номер записи: 98

25-05-2017 дата публикации

BIOTECHNOLOGICAL PRODUCTION OF omega-FUNCTIONALISED CARBOXYLIC ACIDS AND ESTERS THEREOF

Номер: US20170145448A1

Автор: Bruegging Wilhelm, Haas Thomas, Meier Ralf, Schaffer Steffen

Принадлежит: EVONIK DEGUSSA GmbH

There is provided a microbial cell for producing at least one omega-functionalized carboxylic acid ester from at least one alkane, wherein the cell is genetically modified to increase the expression relative to the wild type cell of 1. A microbial cell for producing at least one ω-functionalized carboxylic acid ester from at least one alkane , wherein the cell is genetically modified to increase the expression relative to the wild type cell of{'sub': '1', '(i) Enzyme Ecapable of converting the alkane to the corresponding 1-alkanol;'}{'sub': '2', '(ii) Enzyme Ecapable of converting the 1-alkanol of (i) to the corresponding 1-alkanal;'}{'sub': '3', '(iii) Enzyme Ecapable of converting the 1-alkanal of (ii) to the corresponding alkanoic acid;'}{'sub': '4', '(iv) Enzyme Ecapable of converting the alkanoic acid of (iii) to the corresponding alkanoic acid ester; and'}{'sub': '5', '(v) Enzyme Ecapable of converting the alkanoic acid ester of (iv) to the corresponding ω-hydroxy-alkanoic acid ester,'}{'sub': 20', '24, 'claim-text': [{'sub': '20', 'EAcyl-ACP thioesterase, of EC 3.1.2.14 or EC 3.1.2.22,'}, {'sub': '21', 'EAcyl-CoA thioesterase, of EC 3.1.2.2, EC 3.1.2.18, EC 3.1.2.19, EC 3.1.2.20 or EC 3.1.2.22,'}, {'sub': '22', 'EAcyl-CoA:ACP transacylase,'}, {'sub': '23', 'EPolyketide synthase, and'}, {'sub': '24', 'EHexanoic acid synthase.'}], 'and wherein the cell does not comprise a genetic modification that increases the expression relative to the wild type cell of at least one of the following enzymes E-Eselected from the group consisting of2. The cell according to claim 1 , wherein the cell is further genetically modified to increase the expression relative to the wild type cell of{'sub': '6', '(vi) Enzyme Ecapable of converting the corresponding ω-hydroxy-alkanoic acid ester of (v) to the corresponding ω-oxo alkanoic acid ester; or'}{'sub': 6', '7, '(vii) Enzyme Ecapable of converting the corresponding ω-hydroxy-alkanoic acid ester of (v) to the corresponding ω-oxo ...

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Номер записи: 99

25-05-2017 дата публикации

Improved lipid accumulation in Yarrowia lipolytica strains by overexpression of hexokinase and new strains thereof

Номер: US20170145449A1

Автор: CRUTZ-LE COQ Anne-Marie, DULERMO Thierry, LAZAR Zbigniew, Nicaud Jean-Marc

Принадлежит:

The present invention relates to oleaginous yeast strains overexpressing a hexokinase gene, wherein said strains are capable of accumulating lipids. Methods for obtaining said strains as well as methods for producing lipids are also disclosed. 1Yarrowia lipolytica. A strain overexpressing a hexokinase gene , wherein said strain is of a background selected in the list consisting of A-101 , H222 and W29 , and said strain is capable of accumulating lipids.2. The strain of claim 1 , wherein the said hexokinase gene is ylHXK1.3. The strain of claim 1 , said strain further overexpressing a hexose transporter gene.4. The strain of claim 1 , wherein the said transporter is YHT1 claim 1 , YHT3 or YHT3-1181V.5Saccharomyces cerevisiae.. The strain of claim 1 , said strain further overexpressing the SUC2 gene of6. The strain of claim 1 , said strain further overexpressing the GPD1 gene and said strain being deficient for beta-oxidation of fatty acids.7. The strain of claim 1 , said strain further comprising at least one loss-of-function mutation in at least one gene selected from the PEX genes claim 1 , the POX genes claim 1 , the MFE1 gene claim 1 , and the POT1 gene.8. The strain of claim 1 , said strain further comprising at least one loss-of-function mutation in each of the genes POX1 claim 1 , POX2 claim 1 , POX3 claim 1 , POX3 claim 1 , POX4 claim 1 , POX5 claim 1 , and POX6.9. The strain of claim 1 , said strain further comprising at least one additional mutation in at least one gene encoding an enzyme involved in the metabolism of fatty acids.10. The strain of claim 9 , wherein said mutation further increases the capacity of the strain to accumulate lipids.11. The strain of claim 9 , wherein said mutation is a mutation in GUT2 claim 9 , TLG3 or TLG4.12. The strain of claim 9 , wherein said mutation is a mutation in the YALI0B10153g.13. The strain of claim 1 , said strain further overexpressing the ylDGA2 gene.14. A method for constructing the strain of comprising a step ...

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Номер записи: 100