КОМПОЗИЦИИ И СПОСОБЫ УЛУЧШЕНИЯ МЕДИКАМЕНТОЗНОЙ ТЕРАПИИ СРЕДСТВАМИ, ЗАВИСЯЩИМИ ОТ ИОНОВ МЕТАЛЛОВ

... 1. Способ подготовки пациента к терапевтическому введению металлопротеазы, включающий:рекомендации по введению по меньшей мере одной добавки, которая обеспечивает доступность иона металла на уровне, необходимом для активации терапевтической металлопротеазы, в течение периода нагрузки до, во время и сразу же после введения терапевтической металлопротеазы;причем рекомендованное введение добавки обеспечивает доступность необходимого иона металла на уровне, достаточном для устранения функционального дефицита иона металла как причины плохой или ограниченной реактивности по отношению к вводимой терапевтической металлопротеазе.2. Способ по п.1, отличающийся тем, что терапевтической металлопротеазой является клостридиальный нейротоксин.3. Способ по п.1, отличающийся тем, что добавка содержит цинк в форме, обеспечивающей элементарный цинк в количестве от 10 мг до 400 мг в день.4. Способ по п.3, отличающийся тем, что добавка содержит форму органического цинка, выбранную из одного или более: протеинатов ...

Polypeptid mit Phytaseaktivität und erhöhter Temperaturstabilität der Enzymaktivität sowie dieses codierende Nukleotidsequenz

Die Erfindung betrifft ein rekombinantes DNA-Molekül, das nach Expression in einer prokaryotischen oder eukaryotischen Wirtszelle ein Polypeptid mit Phytaseaktivität codiert, wobei das rekombinante DNA-Molekül eine DNA-Sequenz, ausgewählt aus a) DNA-Sequenzen, die ein Polypeptid mit Phytaseaktivität codieren, das durch Variation der reifen Wildtyp-E. coli-Phytasesequenz erhalten worden ist, wobei die Variation ausgewählt ist aus i) der Mutation Lysin -> Asparaginsäure an Position 74 (K74D), und/oder ii) einer Kombination der Mutationen Asparagin -> Arginin an Position 139 (N139R) und Asparaginsäure -> Glutaminsäure an Position 142 (D142E), und/oder iii) einer Kombination der Mutationen Leucin -> Isoleucin an Position 145 (L145l) und Leucin -> Isoleucin an Position 198 (L198l), und/oder iv) einer Mutation Valin -> Prolin an Position 200 (V200P), und/oder v) einer N-terminalen oder C-terminalen oder einer N- und C-terminalen Anfügung eines Sequenzabschnitts der sauren Phosphatase von Aspergillus ...

Process for converting phytate into inorganic phosphate

TREATMENT OF ÖLSAATEN

REKOMBINANTE BACTERIAL ONE PHYTASEN AND YOUR USE

PROCEDURE FOR UEBERFUEHRUNG OF PHYTAT INTO INORGANIC PHOSPHATE

SALT-STABILIZED ENZYME PREPARATION

High expression cereal phytase gene

The present invention provides mutant cereal plants and mature grain thereof, characterised by enhanced levels of the enzyme phytase in the grain, and methods for inducing, detecting and selecting the mutant cereal plants. The invention further relates to animal feed comprising said grain having enhanced amounts of phytase.

Fusion of bioactive molecules

Fusion-proteins containing an enzyme, preferably a feed or food enzyme, coupled to a gut surface-binding domain are presented. The fusion-proteins can be used to promote feed utilization in animals. In a particular example, a Fusion enzyme according to the invention comprising a gut-surface-binding polypeptide segment linked to a phytase show an increased resident time in the gut, which leads to an increased amount of time given to the enzyme to catalyse the corresponding reaction which finally leads to improved feed utilisation.

RECOMBINANT BACILLUS PHYTASES AND USES THEREOF

Oilseed processing

A method for improving the nutritional value of animal feed

The invention relates to the use of at least one bacterial phytase in combination with one or more protease(s) in animal feed for improving weight gain and/or Feed Conversion Ratio (FCR), wherein the phytase is administered in one or more of the following amounts (dosage ranges): 1'000 FYT /kg feed, 2'000 FYT /kg feed 3'000 FYT /kg feed and wherein the proteaseis administered in one of the following amounts (dosage ranges): 10'000 units/kg feed, 11'000, 12'000, 13'000, 14'000, 15'000, 16'000, 17'000, 18'000, 19'000, 20'000 units/kg feed.

Glycosylation as a stabilizer for phytase

The present teachings provide modified enzymes, preferably phytases, which have increased stability, hypothesized to arise from increased glycosylation. The enzymes can be modified to introduce or increase the number of glycosylation sites in the amino acid sequence, or glycosylation can be increased by the use of specific host production methods, or both. The enzymes of the present teachings have an increased stability after treatment at elevated temperature, which can be measured by inactivity reversibility or percent recovery following a treatment such as heating. The enzymes of the present teachings find application for example in food, feed, and feed pelleting.

Salt-stabilized enzyme preparations

ZINC SUPPLEMENTATION TO INCREASE RESPONSIVENESS TO METALLOPROTEASE THERAPY

Methods and compositions are provided for increasing responsiveness to therapeutic metalloproteases including increasing and/or maximizing responsiveness and preventing botulinum and tetanus toxin resistance due to a functional deficiency of zinc. Also provided are methods for zinc replacement or supplement in lacking individuals comprising the administration of a zinc supplement for a loading period and/or administration of a phytase supplement together with the zinc supplement. Also provided are methods for standardization of botulinum toxin potency assays that provide for greater certainty and margins of safety in the use of products from different manufacturers.

A METHOD FOR IMPROVING THE SOLUBILITY OF VEGETABLE PROTEINS

The present invention relates to a method for improving the solubility of ve getable proteins. More specifically, the invention rela tes to methods for the solubilization of proteins in vegetable protein sources, which methods comprise treating the vegetable protein so urce with an efficient amount of one or more phytase enzymes, and treating the ve getable protein source with an efficient amount of one o r more proteolytic enzymes. In another aspect, the invention provides animal feed additives comprising a phytase and one or more proteolyt ic enzymes.

A METHOD FOR IMPROVING THE SOLUBILITY OF VEGETABLE PROTEINS

The present invention relates to a method for improving the solubility of vegetable proteins. More specifically, the invention relates to methods for the solubilization of proteins in vegetable protein sources, which methods comprise treating the vegetable protein source with an efficient amount of one or more phytase enzymes, and treating the vegetable protein source with an efficient amount of one or more proteolytic enzymes. In another aspect, the invention provides animal feed additives comprising a phytase and one or more proteolytic enzymes.

NOVEL PHYTASE

The invention provides a purified phytate enzyme derived from Escherichia coli B. The enzyme has a molecular weight of about 47.1 kilodaltons and has phytase activity (SEQ ID NO:2). The enzyme can be produced from native or recombinant host cells and can be used to aid in the digestion of phytate where desired. In particular, the phytase of the present invention can be used in animal feed.

PROCEDE DE PREPARATION D'UNE FRACTION DES PROTEINES DU SOJA.

METHOD OF THE MICRO-BIALNI CARRYING OUT OF THE VALUABLE CONNECTION

ПЕРЕРАБОТКА МАСЛИЧНЫХ СЕМЯН

Раздавленные и обезжиренные семена масличных культур экстрагируют водой при нейтральных, умеренно щелочных или умеренно кислых рН и фильтруют. Средство фильтрации позволяет мелким фрагментам клеточного материала проходить в фильтрат. Фильтрат обрабатывают щелочью для повышения рН выше 9 и твердые вещества отделяют. Белки отделяют от других компонентов жидкости и концентрируют, например, индукционным нагреванием или изоэлектрическим осаждением и/или ультрафильтрацией. Оставшаяся жидкость обогащена сахарами. Способ обеспечивает получение одного или более белковых продуктов, пригодных в качестве ингредиентов пищи для человека или корма для животных или косметических продуктов, и богатого сахарами продукта, пригодного для ферментации или в качестве пищевого ингредиента, и волоконно-белкового ингредиента корма животных.

СПОСІБ ВОДНОГО ЕКСТРАГУВАННЯ І ФРАКЦІОНУВАННЯ МАТЕРІАЛУ З ОЛІЙНОГО НАСІННЯ

Спосіб водного екстрагування і фракціонування вихідних матеріалів з олійного насіння, одержаного з насіння рапсу або каноли включає змішування матеріалу з олійного насіння з водним розчином, що має рН більше 2 і менше 12 при концентрації від 10 до 50 % (мас./об.) з утворенням суміші, що містить водний розчин, який включає екстрагований білок, дрібні фрагменти твердих фаз серцевини клітин матеріалу з олійного насіння і матеріал волокон, здійснення розділення одержаної суміші шляхом фільтрації або просівання за допомогою сита з одержанням як фільтрату водного екстрату, що включає екстрагований білок і дрібні фрагменти твердих фаз серцевини клітин, і з одержанням твердого залишку екстрагування, що містить матеріал волокон, обробку зазначеного водного екстракту ензимом, збагаченим фітазою, з одержанням екстрагованої дефітинізованої фракції, збагаченої білком, осадження частини білка в зазначеній екстрагованій дефітинізованій фракції, збагаченій білком, і обробку зазначеної екстрагованої фракції ...

PROCESSING OF OIL-BEARING SEEDS

Fitases bacterianas recombinantes e usos das mesmas

NOVEL GRANULES CONTAINING LIVING ORGANISMS FOR ANIMAL FEED

Novel granules that have improved stability and are capable of being, optionally, subsequently granulated in an apparatus using humid heat and pressure. Said granules containing live matter are protected by a gelatine or a dextrine.

OILSEED PROCESSING

Crushed and de-fatted oilseed is extracted with water at neutral, mildly basic or mildly acidic pH; and is filtered. The filter media allows passage of small fragments of solid cell meat into the filtrate. The filtrate is treated with a base to increase pH to over pH 9, and solids are separated out. Protein is separated from other constituents of the liquid and concentrated, for example by heat-induced or isoelectric precipitation and/or ultrafiltration. The remaining liquid is rich in sugars. The process results in one or more protein products suited for human or animal food ingredients or for production of cosmetics, a sugar rich product suitable for fermentation or use as a feed ingredient, and a fiber-protein animal feed ingredient.

Process for converting phytate into inorganic phosphate

A process is provided for converting phytate in a food into inorganic phosphate. The process comprises the steps of:(i) mechanically mixing a slurry comprising:(a) 100 parts by weight of the phytate-containing food,(b) 60-1000 parts by weight of a solvent mixture which comprises water and a water-immiscible organic solvent having a boiling point of 20-100° C., the water-immiscible organic solvent constituting 20-85% by wt. of the solvent mixture, and(c) a phytase; and(ii) drying the food to remove the organic solvent.The process has the advantage of enabling a high conversion rate of phytate into inorganic phosphate in an economical manner.

Process for hydrolyzing phytate with a synergetic enzyme composition

An enzyme composition having a synergetic phytate hydrolyzing activity comprising a phytase having phytate hydrolyzing activity at a pH of from 2.5 to 5.0 and an acid phosphatase having phytate hydrolyzing activity at a pH of 2.5, in a low ratio corresponding to a pH 2.5/5.0 activity profile of from 0.8/1.0 to 3/1. Said enzyme composition preferably displays a higher synergetic phytate hydrolyzing efficiency through thermal treatment. Fungal enzymes, especially those from Aspergillus, are preferred. Use of said enzyme composition in food, feed and fodder products to improve phytate hydrolysis.

OILSEED PROCESSING

Escherichia coli phytase

The invention provides a purified phytate enzyme derived from Escherichia coli B. The enzyme has a molecular weight of about 47.1 kilodaltons and has phytase activity (SEQ ID NO:2). The enzyme can be produced from native or recombinant host cells and can be used to aid in the digestion of phytate where desired. In particular, the phytase of the present invention can be used in animal feed.

Process for converting phytate into inorganic phosphate

PRODUCTION OF PHYTAT DEGRADIERENDEN ENZYMES IN TRICHODERMA

USE FROM MUTATIONS TO THE IMPROVEMENT OF ASPERGILLUS PHYTASEN

PROCEDURE FOR THE PRODUCTION OF A HIGHLY PURIFIED VEGETABLE PROTEIN WITH LOW RIBONUKLEINSÄURE CONCENTRATION

EXPRESSION OF PHYTASE IN PLANTS

CRACKING AND TREATMENT OF OIL SEED FLOUR

Dietetic soy based product, method for production thereof and use thereof

Feed additive composition

A method for improving the performance of a subject or for improving digestibility of a raw material in a feed (e.g. nutrient digestibility, such as amino acid digestibility), or for improving nitrogen retention, or for improving dietary phosphorus absorption and retention, or for improving the efficacy of the phytase, or for improving the subject's resistance to necrotic enteritis or for improving feed conversion ratio (FCR) or for improving weight gain in a subject or for improving feed efficiency in a subject or for modulating (e.g. improving) the immune response of the subject or for reducing populations of pathogenic bacteria in the gastrointestinal tract of a subject, or for reducing nutrient excretion in manure, which method comprising administering to a subject at least one direct fed microbial in combination with a phytase, wherein the phytase is administered to the subject at a dosage of more than about 1500FTU/kg feed.

OILSEED PROCESSING

Crushed and de-fatted oilseed is extracted with water at neutral, mildly basic or mildly acidic pH; and is filtered. The filter media allows passage of small fragments of solid cell meat into the filtrate. The filtrate is treated with a base to increase pH to over pH 9, and solids are separated out. Protein is separated from other constituents of the liquid and concentrated, for example by heat-induced or isoelectric precipitation and/or ultrafiltration. The remaining liquid is rich in sugars. The process results in one or more protein products suited for human or animal food ingredients or for production of cosmetics, a sugar rich product suitable for fermentation or use as a feed ingredient, and a fiber-protein animal feed ingredient.

PROCESSES FOR INCREASING EXTRACTION OF ENZYMES FROM ANIMAL FEED AND MEASURING ACTIVITY OF THE SAME

Methods of increasing extraction of enzymes from animal feed and measuring enzyme activity are described herein.

THERMOSTABLE PHYTASE VARIANTS

The present invention relates to a method for producing phytase variants which has at least 74% identity to a phytase derived from Citrobacter braakii and comprises at least two additional disulfide bonds as compared to this phytase. These phytase variants have modified, preferably improved, properties, such as thermostability, temperature profile, pH profile, specific activity, performance in animal feed, reduced protease sensitiliby, and/or an modified glycosylation pattern. The invention also relates to the variants produced, DNA encoding these phytases, methods of their production, as well as the use thereof, e.g. in animal feed and animal feed additives.

BIOLOGICAL YEAST, METHOD FOR OBTAINING SAME AND USES THEREOF

La présente invention concerne un procédé de production de levure. Elle concerne en particulier un procédé de production de levurebiologique comprenant l'utilisation de substrats d'originesbiologiquesen particulier un substrat biologique permettant de compléter les besoins nutritionnelsde la levure en phosphore. Le procédé de la présente invention permet l'obtention de levure etextraits de levure biologiques conforment au REGLEMENT (CE) 834/2007de l'Union Européenne. Selon l'invention la composition biologique riche en phosphore est obtenue par hydrolyse et solubilisation d'au moins un substrat végétal d'origine biologique comprenant de 2 à 18g de phosphore par KG de produit dont de 60 à 80% sont sous la forme d'acide phytique. Le substrat préféré selon l'invention est le son de blé.

FEED ADDITIVE COMPOSITION

A feed additive composition comprising a direct fed microbial in combination with a protease, and a phytase, and a method for improving the performance of a subject or for improving digestibility of a raw material in a feed (e.g. nutrient digestibility, such as amino acid digestibility), or for improving nitrogen retention, or for improving the subject's resistance to necrotic enteritis or for improving feed conversion ratio (FCR) or for improving weight gain in a subject or for improving feed efficiency in a subject or for modulating (e.g. improving) the immune response of the subject or for promoting the growth of beneficial bacteria in the gastrointestinal tract of a subject, which method comprising administering to a subject a direct fed microbial in combination with a protease and a phytase.

PRODUCTION OF PHYTATE DEGRADING ENZYMES IN TRICHODERMA

... 2141437 9403612 PCTABS00030 A highly efficient overexpression system for phytase and pH 2.5 acid phosphatase in Trichoderma is described. This system results in enzyme compositions that are especially useful in the animal feed industry.

PRODUCTION OF ENZYMES IN SEEDS AND THEIR USE

A method of catalyzing in vitro reactions using seeds containing enhanced amounts of enzymes is disclosed. The method involves adding transgenic, non-wild type seeds, preferably in a ground form, to a reaction mixture and allowing the enzymes in the seeds to increase the rate of reaction. By directly adding the seeds to the reaction mixture the method provides a solution to the expensive and problematic process of extracting and purifying the enzyme. Methods of treatment are also provided whereby a subject lacking a sufficient supply of an enzyme is administered the enzyme in the form of seeds containing enhanced amounts of the enzyme.

DS11 (KCTC 0231BP), NOVEL BACILLUS SP. STRAIN AND NOVEL PHYTASE PRODUCED BY IT

This invention relates to a novel phytase produced from novel strain Bacillus sp. DS11 (KCTC 0231BP) and more precisely, to a novel strain Bacillus sp. DS11 and phytase enzyme enhancing the phosphate bioavailability present in grains supplied to monogastric animals.

METHOD OF CONVERTING PHYTATE INTO INORGANIC PHOSPHATE, A METHOD OF OBTAINING AN ANIMAL FEED OR HUMAN FOOD

NUTRITIVE COMPOSITION AMELIOREE RESULTING FROM HARDENING FROM BUT AND ITS PROCESS From OBTAINING

L'invention concerne une composition nutritive améliorée résultant de la trempe du maïs caractérisée par le fait qu'elle présente un rapport des concentrations en phosphore inorganique et phosphore total (Pi/Pt) compris entre 35 et 95%, que le dosage des protéines qu'elle contient réalisé selon un test C donne une valeur inférieure ou égale à 5, de préférence inférieure ou égale à 1, et de manière encore plus préférentielle inférieure ou égale à 0,5, et que la teneur en substances réductrices totales est inférieure ou égale à 0,9%. The invention relates to an improved nutritional composition resulting from the quenching of corn, characterized in that it has a ratio of inorganic phosphorus and total phosphorus (Pi / Pt) concentrations of between 35 and 95%, than the determination of the proteins which '' it contains produced according to a test C gives a value less than or equal to 5, preferably less than or equal to 1, and even more preferably less than or equal to 0.5, and that the content of total reducing substances is less than or equal to 0.9%.

VARIABLE IMPROVE Of a PHYTASE

La présente invention relève du domaine de l'amélioration des protéines et en particulier celui des protéines améliorées par évolution moléculaire. Elle porte sur un variant d'une phytase, dit amélioré, en ce qu'il présente une thermostabilité et/ou une activité supérieures à la phytase d'origine. L'invention porte également sur un acide nucléique codant pour ce variant, une cassette ou un vecteur d'expression contenant ce variant, une cellule hôte exprimant ce variant, une composition comprenant ce variant ainsi que les utilisations de ceux-ci, principalement dans la préparation d'additifs alimentaires et l'alimentation animale.

FITASE FROM GERMINATED BEANSES OF SOY

FUSION OF BIOACTIVE MOLECULES

Fusion-proteins containing an enzyme, preferably a feed or food enzyme, coupled to a gut surface-binding domain are presented. The fusion-proteins can be used to promote feed utilization in animals. In a particular example, a Fusion enzyme according to the invention comprising a gut-surface-binding polypeptide segment linked to a phytase show an increased resident time in the gut, which leads to an increased amount of time given to the enzyme to catalyse the corresponding reaction which finally leads to improved feed utilisation.

Thermostable phytase variants

The present invention relates to a method for producing phytase variants which has at least 74% identity to a phytase derived from Citrobacter braakii and comprises at least two additional disulfide bonds as compared to this phytase. These phytase variants have modified, preferably improved, properties, such as thermostability, temperature profile, pH profile, specific activity, performance in animal feed, reduced protease sensitiliby, and/or an modified glycosylation pattern. The invention also relates to the variants produced, DNA encoding these phytases, methods of their production, as well as the use thereof, e.g., in animal feed and animal feed additives.

Phytases, nucleic acids encoding them and methods for making and using them

The invention provides isolated and recombinant phytase enzymes. In one aspect, the phytases are produced by modification of the wild type appA of E. coli. The enzyme can be produced from recombinant host cells. The phytases of the invention can be used to aid in the digestion of phytate where desired. In particular, the phytases of the invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients. The phytases of the invention can be thermotolerant and/or thermostable. Also provided are methods for obtaining a variant polynucleotide encoding a phytase and for obtaining a phytase with thermostability or thermotolerant at high or low temperatures.

Phytase polypeptides

The present invention relates to isolated polypeptides having phytase activity, the corresponding cloned DNA sequences, a process for preparing such polypeptides, and the use thereof for a number of industrial applications. In particular, the invention relates to phytases derived from the phyllum Basidiomycota, phytases of certain consensus sequences and fungal 6-phytases.

Production of enzymes in seeds and their use

A method of catalyzing in vitro reactions using seeds containing enhanced amounts of enzymes is disclosed. The method involves adding transgenic, non-wild type seeds, preferably in a ground form, to a reaction mixture and allowing the enzymes in the seeds to increase the rate of reaction. By directly adding the seeds to the reaction mixture the method provides a solution to the expensive and problematic process of extracting and purifying the enzyme. Methods of treatment are also provided whereby a subject lacking a sufficient supply of an enzyme is administered the enzyme in the form of seeds containing enhanced amounts of the enzyme.

Phytases, nucleic acids encoding them and methods for making and using them

This invention relates to phytases, polynucleotides encoding them, uses of the polynucleotides and polypeptides of the invention, as well as the production and isolation of such polynucleotides and polypeptides. In particular, the invention provides polypeptides having phytase activity under high temperature conditions, and phytases that retain activity after exposure to high temperatures. The phytases of the invention can be thermotolerant and/or thermostable at low temperatures, in addition to higher temperatures. The phytases of the invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients. The phytases of the invention can be formulated as foods or feeds or supplements for either to, e.g., aid in the digestion of phytate. The foods or feeds of the invention can be in the form of pellets, liquids, powders and the like. In one aspect, phytases of the invention are stabile against thermal denaturation during pelleting; and this decreases the cost of the ...

USING MUTATIONS TO IMPROVE ASPERGILLUS PHYTASES

The present invention relates to an isolated nucleic acid molecule encoding a mutant phytase and the isolated mutant phytase itself. The present invention further relates to methods of using the isolated nucleic acid molecule and the isolated mutant phytase of the present invention.

USE OF PHYTASE IN THE PREPARATION OF A FERMENTED SOY BASED PRODUCT

STARCH HYDROLYSIS USING PHYTASE WITH AN ALPHA AMYLASE

Phytate hydrolysis and enzyme composition for hydrolyzing phytate

An enzyme composition having a synergetic phytate hydrolyzing activity comprising a phytase having phytate hydrolyzing activity at a pH of from 2.5 to 5.0 and an acid phosphatase having phytate hydrolyzing activity at a pH of 2.5, in a low ratio corresponding to a pH 2.5/5.0 activity profile of from 0.8/1.0 to 3/1. Said enzyme composition preferably displays a higher synergetic phytate hydrolyzing efficiency through thermal treatment. Fungal enzymes, especially those from Aspergillus, are preferred. Use of said enzyme composition in food, feed and fodder products to improve phytate hydrolysis.

A METHOD FOR IMPROVING THE SOLUBILITY OF VEGETABLE PROTEINS

The present invention relates to a method for improving the solubility of vegetable proteins. More specifically, the invention relates to methods for the solubilization of proteins in vegetable protein sources, which methods comprise treating the vegetable protein source with an efficient amount of one or more phytase enzymes, and treating the vegetable protein source with an efficient amount of one or more proteolytic enzymes. In another aspect, the invention provides animal feed additives comprising a phytase and one or more proteolytic enzymes.

ФРАКЦИОНИРОВАНИЕ И ОБРАБОТКА КОРМОВОЙ МУКИ ИЗ МАСЛИЧНЫХ СЕМЯН

FIELD: food and feed processing industry, in particular production of food proteins and feed meal. SUBSTANCE: method of water extraction and fractionation includes blending of raw material from rape or canola seeds with water in concentration of 10-50 mass/vol % under mild extractive conditions to form aqueous mixture containing aqueous extract including extracted protein, fine fragments of solid phase of seed core cells and fiber material. Further obtained aqueous extract is separated from solid phase residue by filtration or screening through screen to produce filtrate including extracted protein and fine fragments of solid phase of seed core cells residue contains at least 200 mass % of protein calculated as dry matter of solid phase residue. Then said aqueous extract and fine fragments of solid phase of seed core cells are treated with enzyme at 10-70°C to produce extracted dephytinized high protein fraction comprising at least 30 mass % of total protein amount containing in raw materials. Extracted dephytinized fraction is treated by conditioning thereof at not less than 80°C for at least 1 min to induce coagulation of protein containing therein. Further extracted fraction is separated to produce solid phase and liquid. EFFECT: method for production of value feed product for ruminants without side product or wastes. 14 cl, 1 dwg, 2 tbl, 4 ex ÐÎÑÑÈÉÑÊÀß ÔÅÄÅÐÀÖÈß RU (19) (11) 2 275 816 (13) C2 (51) ÌÏÊ A23J 1/14 (2006.01) A23K 1/14 (2006.01) A23K 1/18 (2006.01) ÔÅÄÅÐÀËÜÍÀß ÑËÓÆÁÀ ÏÎ ÈÍÒÅËËÅÊÒÓÀËÜÍÎÉ ÑÎÁÑÒÂÅÍÍÎÑÒÈ, ÏÀÒÅÍÒÀÌ È ÒÎÂÀÐÍÛÌ ÇÍÀÊÀÌ (12) ÎÏÈÑÀÍÈÅ ÈÇÎÁÐÅÒÅÍÈß Ê ÏÀÒÅÍÒÓ (21), (22) Çà âêà: 2002130575/13, 14.05.2001 (24) Äàòà íà÷àëà îòñ÷åòà ñðîêà äåéñòâè ïàòåíòà: 14.05.2001 (73) Ïàòåíòîîáëàäàòåëü(è): ÞÍÈÂÅÐÑÈÒÈ ÎÔ ÑÀÑÊÀÒ×ÅÂÀÍ ÒÅÊÍÎËÎÄÆÈÇ ÈÍÊ. (CA) (43) Äàòà ïóáëèêàöèè çà âêè: 20.08.2004 R U (30) Êîíâåíöèîííûé ïðèîðèòåò: 15.05.2000 US 60/204,120 (72) Àâòîð(û): ÌÀÅÍÖ Äýâèä Ä. (CA), ÍÜÞÊÈÐÊ Ðåêñ Ó. (CA), ÊËÀÑÑÅÍ Ãåíðè Ë. (CA), ÒÀÉËÅÐ Ðîáåðò Ò. (CA) (45) ...

КОМПОЗИЦИИ И СПОСОБЫ УЛУЧШЕНИЯ МЕДИКАМЕНТОЗНОЙ ТЕРАПИИ СРЕДСТВАМИ, ЗАВИСЯЩИМИ ОТ ИОНОВ МЕТАЛЛОВ

Изобретение относится к подготовительному набору, предоставляемому пациенту перед введением терапевтического ботулинического токсина. Заявленный набор содержит добавку в форме капсул или таблеток, которые включают ионы цинка в органической или неорганической форме в дозе, достаточной для потребления от 10 мг до 400 мг элементарного цинка в день, и добавку фитазы в количестве 0,8-10000 единиц, достаточном для введения вместе с добавкой ионов цинка в течение периода нагрузки ионами цинка. Доза потребления цинка обеспечивает активизацию терапевтического ботулинического токсина в течение периода нагрузки ионами цинка перед лечением пациента терапевтическим ботулиническим токсином. Подготовительный набор также содержит инструкции, рекомендующие пациенту принимать добавки ионов цинка и снизить прием фитатов. 3 з.п. ф-лы, 1 табл., 3 ил., 2 пр.

СПОСОБ КАТАЛИЗА РЕАКЦИИ ФЕРМЕНТАЦИИ СУБСТРАТА, СПОСОБ УЛУЧШЕНИЯ ПОТРЕБЛЕНИЯ ПИЩЕВОГО РАЦИОНА ЖИВОТНЫМ, ПЛАЗМИДА pMOG413, ПЛАЗМИДА pMOG429 И ПЛАЗМИДА pMOG227

FIELD: genetic engineering. SUBSTANCE: transgenic plants are obtained and their seeds are added to reacting mixture containing a substrate and an enzyme. Seeds are added as a ground form directly. Form of product ensures to use its for treatment as a food ration. For preparing transgenic plants plasmids are used. Invention can be used for preparing enzymes and in food production. EFFECT: decreased cost due to increase of fermentation rate, simplified extraction and purification of product. 16 cl, 13 dwg, 3 tbl, 16 ex КОММ С03 1991-289815/40 =КО 2129609-С1 5сефз сопие. епрапсеЧ епхуте |еуе|5 Нот 1гапзрепс Рапё5 - изе4 юг сага]узшя геасноп$, шсгеазте пи- итаопа| уа[ие$ ог {теаыпе Феезнуе Фвог4ег СЬТ-ВКОСАПЕ$ МУ 1990.03.23 199005-498561 Во4 016 РО9 Р13 (213) (1999.04.27) *ЕР 449376-А С12М 9/00, Аб1К 31/165, С12М 15/52, 15182, Аб1К 38/43 1991.03.25 199150-5010480 1991.03.25 1991-№И.00048 Вазе4 оп /09114772-А Аа4п].Рага: МОСЕМ ПМТ МУ (МОСЕ-) 1991-289814/40 Тве гоПо\лпв аге саппе4д: (А) а тефо4 оё сага|у$1пр, ап ш уйго геасноп сВагастег1зе4 п (ае зее45 оБа. {гот сгапзрегис р|апиз, Ше ее $ сопие. ап епрапсе аше. о ап епгуте о# пиегезе, аге а44е4 то а геасНоп пхЕ. сопЕр. а забзгате, \уВеге фе епхуте оЁ пиегезе 15 сарае оё сага]уз1е а геасноп оЁ Ве заБгате 11 Ще геасНоп пихе.; (В) а шефо4 оЁ пирго\ар Фе пиннопа! шкаке оЁ ап апипа! от а руеп Ю04 ог {ее4зтой, сВагастезе ш фаг зее 4$ оБ4. от сгапзрегис р]апз, Бе зее $ сопёе. ап епВапсе@ ага. оЁ ап ептуше оЁ пиегезе, аге сотЫште4 \миБ фе юо4 ог #ее4знай, мВеге Фе епхуте оЁ ицегезе 15 сараЫе оё сага!узшв а ЧвезИуе геасНоп т фе Ююо4 ог еедзнаЕ, мсЬ Фрезйуе геасцоп гези$ п 1шсгеаяпв пиы- попа! сотропеп$ ш фе юо4 \рсЬ аге ауаЙаЫе го бе апта] ш5е5Ипр, (пе оо4 ог Еее ф5еаЁ; (С) а тефо4 оЁ хеайпр ап апипа| зайенае гот а Ч1везйуе Ч1зог4ег; (2) а тео оЁ сага|узше ш мо геасноп$; (Е) а сотрзп. сБагастетзе {п "Баг { сопта1пз зее4з оы4. от гапзвегис р!апт$, фе 5ееф$ сопр. ап епрапсе@ апе. оЁап епхуте оЁ итегезс; (Е) а тапзрегис зее4- ...

Verfahren zur Herstellung eines hochgereinigten pflanzlichen Proteins mit niedriger Ribonukleinsäure- Konzentration

Medicament or nutritional supplement containing phytic acid cleaving reagent, preferably phytase, useful for increasing bioavailability of bioelements, e.g. calcium and iron, and combating deficiency diseases

A novel composition (A), preferably in the form of a pharmaceutical composition, medicament or nutritional supplement, contains at least one phytic acid cleaving reagent (I) (preferably an enzyme, i.e. phytase (I'), optionally together with auxiliaries and/or carriers.

PENIOPHORA PHYTASE

Feed additive composition

PRODUCTION OF ENZYMES IN SEEDS AND YOUR USE

POLYPEPTIDE WITH PHYTASE ACTIVITY

Polypeptides having phytase activity and nucleic acids encoding same

METHOD FOR PRODUCING SUBSTRATE CULTURE PRODUCT AND SUBSTRATE CULTURE PRODUCT

Provided is a method for safely and selectively producing a substrate culture product including a large amount of a desired degrading enzyme. A method for producing a substrate culture product used for feedstuff includes inoculating filamentous bacterium bred so that a target degrading enzyme is produced by self-cloning in high productivity on a substrate, and producing the substrate culture product having functionality by ventilating the substrate to carry out solid culture. Selected drawing: FIG. 1 ...

BUTTIAUXELLA SP. PHYTASE VARIANTS

Provided herein are variants of Buttiauxella sp. phytases that may be used in industrial applications including methods for starch liquefaction, alcohol fermentations and for enhancing phosphate digestion in foods and animal feeds.

USING MUTATIONS TO IMPROVE ASPERGILLUS PHYTASES

The present invention relates to an isolated nucleic acid molecule encoding a mutant phytase and the isolated mutant phytase itself. The present invention further relates to methods of using the isolated nucleic acid molecule and the isolated mutant phytase of the present invention.

HAFNIA PHYTASE

The present invention relates to isolated polypeptides having phytase act ivity and isolated polynucleotides encoding the polypeptides. The polypeptid es are related to a phytase derived from Hafnia alvei, the amino acid sequen ce of which is shown in the appended sequence listing as SEQ ID NO: 10. The invention also relates to nucleic acid constructs, vectors, and host cells c omprising the polynucleotides as well as methods for producing and using the polypeptides, in particular within animal feed.

SALT-STABILIZED ENZYME PREPARATIONS

The present invention discloses methods to improve the processing and storage stability of dry enzyme preparations. To this extend an inorganic salt, e.g. MgSO4, is dissolved in an enzyme containing solution which is subsequently dried, using e.g. spray drying. Not only does the addition of salt improve the yield of enzyme activity upon drying, also the storage and processing stability of the obtained solid composition containing the enzyme and the inorganic salt are improved with respect to enzyme activity. The invention further discloses solid compositions comprising enzymes and inorganic salts.

DS11 (KCTC 0231BP), NOVEL BACILLUS SP. STRAIN AND NOVEL PHYTASE PRODUCED BY IT

This invention relates to a novel phytase produced from novel strain Bacillu s sp. DS11 (KCTC 0231BP) and more precisely, to a novel strain Bacillus sp. DS 11 and phytase enzyme enhancing the phosphate bioavailability present in grains supplied to monogastric animals.

Feed additive composition

New pellets containing of the organizations living for the animal feeds

La présente invention concerne de nouveaux granulés présentant une stabilité améliorée capables de pouvoir, éventuellement, subir une granulation ultérieure dans un appareil utilisant comme moyen de granulation la chaleur humide et la pression. Ces granulés contenant des matières vivantes sont protégés par de la gélatine et/ou une dextrine.

SITE-DIRECTED MUTAGENESIS OF ESCHERICHIA COLI PHYTASE

The present invention relates to an isolated mutant acid phosphatase/phytase with improved enzymatic properties. The mutant acid phosphatase/phytase composition is particularly useful in animal feed compositions. © KIPO & WIPO 2007 ...

PHYTASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING THEM

The invention provides isolated and recombinant phytase enzymes. In one aspect, the phytases are produced by modification of the wild type appA of E.coli. The enzyme can be produced from recombinant host cells. The phytases of the invention can be used to aid in the digestion of phytate where desired. In particular, the phytases of the invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients. The phytases of the invention can be thermotolerant and/or thermostable. Also provided are methods for obtaining a variant polynucleotide encoding a phytase and for obtaining a phytase with thermostability or thermotolerant at high or low temperatures. © KIPO & WIPO 2007 ...

PHYTASE FORMULATION

RECOMBINANT BACTERIAL FITASES AND THE USES OF THE SAME ONES

POLYPEPTIDE HAVING PHYTASE ACTIVITY AND INCREASED TEMPERATURE RESISTANCE OF THE ENZYME ACTIVITY, AND NUCLEOTIDE SEQUENCE CODING SAID POLYPEPTIDE

Die Erfindung betrifft ein rekombinantes DNA-Molekül, das nach Expression in einer prokaryotischen oder eukaryotischen Wirtszelle ein Polypeptid mit Phytaseaktivität codiert, wobei das rekombinante DNA-Molekül eine DNA-Sequenz, ausgewählt aus a) DNA-Sequenzen, die ein Polypeptid mit Phytaseaktivität codieren, das durch Variation der reifen Wildtyp-E. co/i-Phytasesequenz erhalten worden ist, wobei die Variation ausgewählt ist aus i) der Mutation Lysin → Asparaginsäure an Position 74 (K74D), und/oder ii) einer Kombination der Mutationen Asparagin → Arginin an Position 139 (N139R) und Asparaginsäure → Glutaminsäure an Position 142 (D142E), und/oder iii) einer Kombination der Mutationen Leucin → Isoleucin an Position 145 (L145I) und Leucin → Isoleucin an Position 198 (L198I), und/oder iv) einer Mutation Valin → Prolin an Position 200 (V200P), und/oder v) einer N-terminalen oder C-terminalen oder einer N- und C-terminalen Anfügung eines Sequenzabschnitts der sauren Phosphatase von Aspergillus niger var. awamori oder der Phytase von Aspergillus niger, b) DNA-Sequenzen, die eine Homologie von 70% bis 100% zu den Sequenzen gemäß a) aufweisen, c) DNA-Sequenzen, die aufgrund der Degeneriertheit des genetischen Codes mit den Sequenzen gemäß a) und b) verwandt sind, umfasst, wobei das rekombinante DNA-Molekül bei Expression in einer geeigneten Wirtszelle mit einer erhöhten Temperatur- und Proteasestabilität der Enzymaktivität des so codierten Proteins einhergeht, wobei die Variationen iii) und iv) nur in Kombination mit einer Variation i), ii) und v) vorliegen sowie die dadurch codierten Proteine.

Phytase polypeptides

The present invention relates to isolated polypeptides having phytase activity, the corresponding cloned DNA sequences, a process for preparing such polypeptides, and the use thereof for a number of industrial applications. In particular, the invention relates to phytases derived from the phyllum Basidiomycota, phytases of certain consensus sequences and fungal 6-phytases.

SPORE SURFACE DISPLAYS OF BIOACTIVE MOLECULES

This invention discloses novel bacterial spore systems. It has now been found surprisingly that under certain conditions bacterial spore systems can be used in the food and feed industry, preferably in animal feeding and as biohybrid material. More precisely applicant has found the following: Genetically modified or genetically engineered viable spore systems expressing bioactive polypeptides, for example bacteriocins and/or enzymatically active feed enzymes, at the spore surface, have a great potential use in animal feeding. Further, it has been found that genetically modified or genetically engineered inert spore systems expressing affinity ligands or immobilized enzymes at the surface have a great potential use in biocatalysis and in the construction of biocatalytic films. Especially the resistance to harsh chemicals, desiccation, strong pressure, or high temperatures allows the spores to be a potentially valuable tool for the display of bioactive molecules, like biocatalytic enzymes ...

A method for improving the solubility of vegetable proteins

PROCEDURE FOR A SWITCHES OF GETREIDEN WITH A NEW ENZYME COMPOSITION.

PROCEDURE FOR THE PRODUCTION OF A DRIED SCHNELLKOCHHÜLSENFRÜCHTEPÜREES

PENIOPHORA PHYTASE

APPROACHING COMPOSITION FROM THE CORN SPRING WATER AND PROCEDURE FOR THEIR PRODUCTION

PHYTATE HYDROLYSIS AND ENZYMATIC COMPOSITION FOR THE HYDROLYSIS OF PHYTAT

POLYPEPTIDE WITH PHYTASE ACTIVITY AND IT CODING NUCLEIC ACIDS

NEW PHYTASE

Recombinant bacterial phytases and uses thereof

Feed additive composition

A feed additive composition comprising a direct fed microbial in combination with a protease, and a phytase, and a method for improving the performance of a subject or for improving digestibility of a raw material in a feed (e.g. nutrient digestibility, such as amino acid digestibility), or for improving nitrogen retention, or for improving the subject's resistance to necrotic enteritis or for improving feed conversion ratio (FCR) or for improving weight gain in a subject or for improving feed efficiency in a subject or for modulating (e.g. improving) the immune response of the subject or for promoting the growth of beneficial bacteria in the gastrointestinal tract of a subject, which method comprising administering to a subject a direct fed microbial in combination with a protease and a phytase.

High expression cereal phytase gene

The present invention provides mutant cereal plants and mature grain thereof, characterised by enhanced levels of the enzyme phytase in the grain, and methods for inducing, detecting and selecting the mutant cereal plants. The invention further relates to animal feed comprising said grain having enhanced amounts of phytase.

A method for improving the solubility of vegetable proteins

PROCESS FOR CONVERTING PHYTATE INTO INORGANIC PHOSPHATE

A process is provided for converting phytate in a food into inorganic phosphate. The process comprises the steps of: (i) mechanically mixing a slurry comprising: (a) 100 parts by weight of the phytate-containing food, (b) 60-1000 parts by weight of a solvent mixture which comprises water and a water-immiscible organic solvent having a boiling point of 20-100 .degree.C, the water-immiscible organic solvent constituting 20-85 % by wt. of the solvent mixture, and (c) a phytase; and (ii) drying the food to remove the organic solvent. The process has the advantage of enabling a high conversion rate of phytate into inorganic phosphate in an economical manner.

FRACTIONATION AND PROCESSING OF OILSEED MEAL

The present invention relates to a process for the aqueous extraction, fractionation and enzymatic treatment of oilseed materials to generate valued products with no significant low value by-product or waste streams. In particular, the fractionation scheme generates a protein-fibre feed ingredient principally for use with ruminant animals and a second dephytinized high protein fraction. The dephytinized high protein fraction has value as feed ingredient for a variety of species of animals.

NOVEL PHYTASES AND USES THEREOF

The present invention relates to variant phytase enzymes and their use thereof. 1. A composition comprising a variant phytase enzyme comprising at least one amino acid substitutions as compared to SEQ ID NO:1 , wherein said amino acid substitution is at a position number selected from the group consisting of 255 , 159 , 354 , 1 , 30 , 36 , 39 , 55 , 60 , 65 , 69 , 73 , 74 , 79 , 85 , 101 , 109 , 111 , 116 , 118 , 120 , 137 , 138 , 139 , 141 , 146 , 157 , 176 , 180 , 183 , 184 , 185 , 186 , 189 , 233 , 245 , 276 , 282 , 288 , 291 , 295 , 297 , 311 , 315 , 341 , 363 , 369 , 370 , 380 , 383 , 385 and 402.2. A composition comprising a variant phytase enzyme comprising at least one amino acid substitutions as compared to SEQ ID NO:1 , wherein said amino acid substitution is at a position number selected from the group consisting of 255 , 159 , 354 , 1 , 30 , 36 , 39 , 55 , 60 , 65 , 69 , 73 , 74 , 79 , 85 , 101 , 109 , 111 , 116 , 118 , 120 , 137 , 138 , 139 , 141 , 146 , 157 , 176 , 180 , 183 , 184 , 185 , 186 , 189 , 233 , 245 , 276 , 282 , 288 , 291 , 295 , 297 , 311 , 315 , 341 , 363 , 369 , 370 , 380 , 383 , 385 and 402 , wherein said variant phytase enzyme has at least at least 1.1 fold better activity as compared to SEQ ID NO:1 under a condition selected from the group consisting of thermostability at 58° C. , thermostability at 66° C. , pH stability at pH 4.5 and pH stability at pH 5.5.3. A composition according to wherein said variant phytase enzyme is at least 95% identical to SEQ ID NO:1.4. A composition according to wherein said amino acid substitutions are selected from the group consisting of Y255D claim 1 , R159Y claim 1 , F354Y claim 1 , Q1S claim 1 , Q1V claim 1 , Q1N claim 1 , Q30K claim 1 , A36K claim 1 , T39D claim 1 , I55V claim 1 , H60S claim 1 , H60Q claim 1 , R65H claim 1 , D69N claim 1 , A73D claim 1 , A73E claim 1 , K74D claim 1 , K74P claim 1 , K74L claim 1 , Q79L claim 1 , Q79R claim 1 , Q79A claim 1 , Q79G claim 1 , Q79F claim 1 , I85V claim ...

PHYTASE FORMULATION

A liquid enzyme formulation, an enzyme granule formulation, methods for manufacturing enzyme granules using a fluid bed dryer, wherein the enzyme granules are thermostable without the need for a thermostable coating is provided. The enzyme granules are phytase granules used in the manufacturing of an animal feed, wherein the phytase granule is thermostable without the need for a thermostable coating and the phytase retains about 63% to about 134% of its activity after pelleting at 80° C. 1. A liquid enzyme formulation comprising:(a) a phytase;(b) a buffer, wherein the buffer is selected from the group consisting of: a sodium citrate, potassium citrate, citric acid, sodium acetate, acetic acid, sodium phosphate, potassium phosphate and any combination thereof;(c) a stabilizer, wherein the stabilizer is selected from the group consisting of: a sucrose, a sorbitol, a mannitol, a glycerol, a trehalose, a sodium chloride, a sodium sulphate, or any combination thereof; and,(d) an anti-microbial, wherein the anti-microbial is selected from the group consisting of: a potassium sorbate, sodium sorbate, a sorbic acid, a sodium benzoate, a benzoic acid, a methyl paraben, a calcium propionate, a sodium propionate, an ammonium propionate, a propionic acid, or any combination thereof.2. The liquid enzyme formulation of claim 1 , wherein(a) the buffer has a pH in a range from about 4.5 to about 5.50,(b) the stabilizer is a combination of at least two stabilizers,(c) the stabilizer is at a concentration between about 5% to about 30%, or(d) the anti-microbial is a combination of at least two anti-microbials.322.-. (canceled)23. A granulated enzyme formulation comprising:(a) a carrier;(b) a primary solution comprising a phytase, a buffer, a stabilizer, a binding agent, a first plasticizer, an anti-microbial, or any combination thereof; and(c) a secondary solution comprising an enhancer, a second plasticizer, or any combination thereof.24. The granulated enzyme formulation of claim 23 ...

Glycosylation as a stabilizer for phytase

The present teachings provide modified enzymes, preferably phytases, which have increased stability, hypothesized to arise from increased glycosylation. The enzymes can be modified to introduce or increase the number of glycosylation sites in the amino acid sequence, or glycosylation can be increased by the use of specific host production methods, or both. The enzymes of the present teachings have an increased stability after treatment at elevated temperature, which can be measured by inactivity reversibility or percent recovery following a treatment such as heating. The enzymes of the present teachings find application for example in food, feed, and feed pelleting.

Phytase having improved enzymatic activity

A phytase having improved enzymaic activity is disclosed. The phytase has a modified amino acid sequence of SEQ ID NO: 2, wherein the modification is a substitution of Valine at position 90 with Threonine. Alternatively, the phytase has a modified amino acid sequence of SEQ ID NO: 6, wherein the modification is a substitution of Asparagine at position 204 with Alanine or a substitution of Serine at position 206 with Alanine.

Polypeptides Having Phytase Activity

The present invention discloses novel phytases that have improved phytase activity, compositions comprising them, recombinant host cells suitable for their production, and their use in feed applications.

Compositions and Methods for Enhancing Metal Ion Dependent Drug Therapies

Methods and compositions are provided for increasing responsiveness to therapeutic metalloproteases including increasing and/or maximizing responsiveness and preventing botulinum and tetanus toxin resistance due to a functional deficiency of zinc. Also provided are methods for zinc replacement or supplement in lacking individuals comprising the administration of a zinc supplement for a loading period and/or administration of a phytase supplement together with the zinc supplement. Also provided are methods for standardization of botulinum toxin potency assays that provide for greater certainty and margins of safety in the use of products from different manufacturers. 1: A plurality of supplements in powder , liquid , liquid-gel , capsule or tablet form provided to a patient to prepare the patient for treatment with a metalloprotease , wherein the supplements comprise a combination of metal ions required for activity of the metalloprotease and phytase , wherein the metal ions and the phytase are provided in the combination in a quantity sufficient to supply and render available the metal ions daily for a loading period immediately prior to treatment of the patient with the metalloprotease and thereby eliminate a functional deficiency of the metal ions as a cause for poor or limited responsiveness to the administered metalloprotease.2: The plurality of supplements of claim 1 , wherein the metalloprotease is a Clostridial neurotoxin.3: The plurality of supplements of claim 2 , wherein the metal ions comprise an organic or inorganic form of zinc at a dosage sufficient to supply 10 to 400 mg of elemental zinc daily for a loading period immediately prior to treatment of the patient with the Clostridial neurotoxin.4: The plurality of supplements of claim 1 , wherein the co-formulated supplements provide 0.8 to 10 claim 1 ,000 units of phytase to the patient daily.5: The plurality of supplements of claim 1 , wherein the loading period is at least 4 days immediately prior to ...

HIGH EXPRESSION CEREAL PHYTASE GENE

The present invention provides mutant cereal plants and mature grain thereof, characterised by enhanced levels of the enzyme phytase in the grain, and methods for inducing, detecting and selecting the mutant cereal plants. The invention further relates to animal feed comprising said grain having enhanced amounts of phytase. 115-. (canceled)16Tricticum: A variety capable of producing an average phytase endosperm content of greater than 4300 FTU/kg , produced by the following:{'i': 'Tricticum', 'a) obtaining a plant which has been identified as having a nucleotide at the 5′ end of a polynucleotide having the nucleotide sequence ACA VGA GTC ATG CAT [SEQ ID NO:1] wherein V is a cytosine.'}{'i': Tricticum', 'Tricticum, 'b) breeding said plant comprising said nucleotide sequence with a second variety plant which produces an average phytase content of about 1200 FTU/kg, to produce grains,'}{'i': 'Tricticum', 'c) growing at least one plant from said grains;'}d) assaying said grains to confirm the presence of said nucleotide sequence in that said variety produces an average phytase endosperm content of greater than 4300 FTU/kg.17Tricticum: The variety of wherein said plant has been identified by{'i': 'Tricticum', 'a) obtaining a sample of nucleic acids from a plant or portion thereof;'}b) detecting in said sample the presence of the nucleotide t the 5′ end of a polynucleotide having the nucleotide sequence ACA VGA GTC ATG CAT [SEQ ID NO:1] wherein V is a cytosine.18: The plant or variety according to claim 17 , wherein said sample of nucleic acids comprises a first polynucleotide located 5′ upstream of and operably linked to a second polynucleotide claim 17 , wherein said first polynucleotide comprises the nucleotide sequence ACA VGA GTC ATG CAT (SEQ ID NO:1) [SEQ ID NO:1] wherein V is a cytosine claim 17 , and wherein said second polynucleotide encodes a phytase polypeptide having myo-inositol hexakisphosphate phosphohydrolase activity.19Tricticum: A population of plants ...

PHYTASE FORMULATION

A liquid enzyme formulation, an enzyme granule formulation, methods for manufacturing enzyme granules using a fluid bed dryer, wherein the enzyme granules are thermostable without the need for a thermostable coating is provided. The enzyme granules are phytase granules used in the manufacturing of an animal feed, wherein the phytase granule is thermostable without the need for a thermostable coating and the phytase retains about 63% to about 134% of its activity after pelleting at 80° C. 1. A granulated enzyme formulation comprising:(a) a carrier;(b) a primary solution comprising a phytase, a buffer, a stabilizer, a binding agent, a first plasticizer, an anti-microbial, or any combination thereof; and(c) a secondary solution comprising an enhancer, a second plasticizer, or any combination thereof.2. The granulated enzyme formulation of claim 1 , wherein the carrier is selected from the group consisting of:(a) a flour;(b) a wheat flour;(c) a bleached flour(d) a wheat flour and a maltodextrin;(e) a wheat flour and a pre-gelatinized starch; and(f) a starch.3. The granulated enzyme formulation of claim 1 , wherein the buffer is a sodium citrate.4. The granulated enzyme formulation of claim 1 , wherein the stabilizer is selected from the group consisting of: a sucrose claim 1 , a sorbitol claim 1 , a mannitol claim 1 , a glycerol claim 1 , a trehalose claim 1 , a sodium chloride claim 1 , a sodium sulphtate claim 1 , kaolin claim 1 , aluminum silicate claim 1 , magnesium silicate claim 1 , and any combination thereof.5. The granulated enzyme formulation of claim 1 , wherein the binding agent is selected from the group consisting of: a guar gum claim 1 , a xanthan claim 1 , a sodium algenate claim 1 , a locust bean gum claim 1 , a carrageenan gum claim 1 , a pre-gelatinized modified starch claim 1 , a maltodextrin claim 1 , gelatin claim 1 , a methyl celluloase claim 1 , a hydroxypropyl cellulose claim 1 , a hydroxypropyl methyl celluloase claim 1 , a carboxymethyl ...

A Method for Improving the Nutritional Value of Animal Feed

The invention relates to the use of at least one bacterial phytase in combination with one or more protease(s) in animal feed for improving weight gain and/or Feed Conversion Ratio (FCR), wherein the phytase is administered in one or more of the following amounts (dosage ranges): 1,000 FYT/kg feed, 2,000 FYT/kg feed 3,000 FYT/kg feed and wherein the protease is administered in one of the following amounts (dosage ranges): 10,000 units/kg feed, 11,000, 12,000, 13,000, 14,000, 15,000, 16,000, 17,000, 18,000, 19,000, 20,000 units/kg feed. 1. A method for increasing weight gain and/or improving Feed Conversion Ratio of farm animals , the method comprising the step of applying to the animal a feed with an efficient amount of one or more proteolytic enzymes in combination with at least one phytase wherein:a. the phytase is administered in such amounts that the specific activity in the final feed is between 1000 FYT/kg feed and 4000 FYT/kg feed andb. the protease is administered in a dosage of between 10,000 units/kg feed and 30,000 units/kg feed.2. The method of claim 1 , wherein:a. the phytase is administered in one or more of the following amounts: 1000, 2000 or 3000 FYT/kg feed andb. the protease is administered in one of the following amounts: 10,000 units/kg feed, 11,000, 12,000, 13,000, 14,000, 15,000, 16,000, 17,000, 18,000, 19,000, 20,000 units/kg feed.3. The method according to claim 2 , wherein the enzymes are administered as follows:a. Phytase: 3000 FYT/kg feed andb. Protease: 15,000 units/kg feed.4. The method according to claim 1 , wherein the phytase is classified as belonging to the EC 3.1.3.26 group.5. The method according to claim 1 , to wherein the phytase is derived from the family Enterobacteriaceae.6. The method according to claim 1 , wherein the protease is an acid stable serine protease obtained or obtainable from the class Actinobacteria.7Nocardiopsis dassonvilleidassonvilleiNocardiopsis prasinaNocardiopsis prasinaalbaNocardiopsisNocardiopsis ...

PHYTASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING THEM

This invention relates to phytases, polynucleotides encoding them, uses of the polynucleotides and polypeptides of the invention, as well as the production and isolation of such polynucleotides and polypeptides. In particular, the invention provides polypeptides having phytase activity under high temperature conditions, and phytases that retain activity after exposure to high temperatures. The phytases of the invention can be thermotolerant and/or thermostable at low temperatures, in addition to higher temperatures. The phytases of the invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients. The phytases of the invention can be formulated as foods or feeds or supplements for either to, e.g., aid in the digestion of phytate. The foods or feeds of the invention can be in the form of pellets, liquids, powders and the like. In one aspect, phytases of the invention are stabile against thermal denaturation during pelleting; and this decreases the cost of the phytase product while maintaining in vivo efficacy and detection of activity in feed. 142-. (canceled)43. A variant polypeptide comprising an amino acid sequence as set forth in SEQ ID NO:2 and one single amino acid substitution to the amino acid sequence as set forth in SEQ ID NO:2 , wherein said one single amino acid substitution is selected from the group consisting of: C97E , C97V , E168R , and C226D , wherein said variant polypeptide has phytase activity.44. A protein preparation comprising the variant polypeptide as set forth in claim 43 , wherein the protein preparation comprises a liquid claim 43 , a slurry claim 43 , a powder claim 43 , a spray claim 43 , a suspension claim 43 , a lyophilized composition/formulation claim 43 , a solid claim 43 , geltab claim 43 , pill claim 43 , implant claim 43 , a gel; or a pharmaceutical formulation claim 43 , a food or a feed or a supplement thereof.45. A food or feed claim 43 , or a food or feed supplement claim 43 , for an animal or ...

Buttiauxella sp. phytase variants

Provided herein are variants of Buttiauxella sp. phytases that may be used in industrial applications including methods for starch liquefaction, alcohol fermentations and for enhancing phosphate digestion in foods and animal feeds.

BUTTIAUXELLA SP. PHYTASE VARIANTS

Provided herein are variants of sp. phytases that may be used in industrial applications including methods for starch liquefaction, alcohol fermentations and for enhancing phosphate digestion in foods and animal feeds. 1Buttiauxella. A phytase variant having improved thermal activity of at least about 5° C. as compared to wild type sp. phytase (SEQ ID NO: 1).2. The phytase variant of claim 1 , wherein the improved thermal activity is at least about 17° C. as compared to the phytase SEQ ID NO: 1.3. The phytase variant of or claim 1 , wherein the improved thermal activity is in a range from about 17 to about 21° C.4. The phytase variant of any one of to claim 1 , wherein the improved thermal activity is in a range from about 17.4 to about 20.2° C.5Buttiauxella. A thermostable phytase variant capable of retaining greater than 50% of its activity after exposure to an elevated temperature for 10 minutes at pH 5.5 in buffer claim 1 , wherein the elevated temperature is at least about 5° C. higher than a temperature at which wild type sp. phytase (SEQ ID NO: 1) retains greater than 50% of its activity.6. The thermostable phytase variant of claim 5 , wherein the elevated temperature is at least about 20.5° C. higher.7. The thermostable phytase variant of or claim 5 , wherein the elevated temperature is in a range from about 20.5° C. to about 27° C.8. The thermostable phytase variant of any one of to claim 5 , wherein the elevated temperature is in a range from about 20.2° C. to about 26.8° C.9Buttiauxella. A phytase variant having phytase activity and an amino acid sequence that varies from the amino acid sequence of wild type sp. phytase (SEQ ID NO: 1) claim 5 , wherein the amino acid sequence of the phytase variant comprises a variation at one or more positions corresponding to position 75 claim 5 , 76 claim 5 , 77 claim 5 , 198 claim 5 , 367 or 374 of SEQ ID NO: 1.10. The phytase variant of claim 9 , wherein the phytase variant further comprises one or more additional ...

THERMOSTABLE PHYTASE VARIANTS

The present invention relates to a method for producing phytase variants which has at least 74% identity to a phytase derived from and comprises at least two additional disulfide bonds as compared to this phytase. These phytase variants have modified, preferably improved, properties, such as thermostability, temperature profile, pH profile, specific activity, performance in animal feed, reduced protease sensitiliby, and/or an modified glycosylation pattern. The invention also relates to the variants produced, DNA encoding these phytases, methods of their production, as well as the use thereof, e.g., in animal feed and animal feed additives. 136-. (canceled)37. A method of producing a variant phytase having at least 74% identity to SEQ ID NO:2 and comprising the establishment of at least two disulfide bridges as compared to SEQ ID NO:2 , wherein said two disulfide bridges are not among the four naturally occurring ones in positions 77/108 , 133/407 , 178/187 , and 381/390 with the numbering as provided in SEQ ID NO:2.38. The method of claim 37 , wherein the at least two disulfide bridges are established in positions selected from the group consisting of the position pairs: A) 52/99; B) 31/177; C) 46/91; D) 141/199; E) 31/176; F) 59/100; and G) 162/247.39. The method of claim 37 , wherein the first disulfide bridge is established in the position pair A between the residues in positions 52 and 99.40. The method of claim 37 , wherein the second disulfide bridge is established in the position pair B between the residues in positions 31 and 177.41. The method of claim 37 , wherein the number of established disulfide bridges is 2 claim 37 , 3 claim 37 , 4 claim 37 , 5 claim 37 , 6 claim 37 , and/or 7.42. The method of claim 37 , wherein the number of established disulfide bridges is two using the following combinations of position pairs:A+B, A+C, A+D, A+E, A+F, A+G, B+C, B+D, B+E, B+F, B+G, C+D, C+E, C+F, C+G, D+E, D+F, D+G, E+F, E+G, and F+G, wherein A means 52/99; B ...

Polypeptide having phytase activity and increased temperature resistance of the enzyme activity, and nucleotide sequence coding said polypeptide

The invention relates to a recombinant DNA molecule encoding a polypeptide having phytase activity and increased temperature stability and increased proteolytic stability of the enzyme activity. The DNA sequence has been obtained by variation of the mature wild-type E. coli phytase sequence with defined amino acid positions being modified in comparison to the wild-type sequence or with the sequences having N- and/or C-terminal extensions, respectively. The invention further relates to a method for expressing the recombinant phytase as well as its use in the food and animal feed technologies.

Feed additive composition

A method for improving the performance of a subject or for improving digestibility of a raw material in a feed (e.g. nutrient digestibility, such as amino acid digestibility), or for improving nitrogen retention, or for improving dietary phosphorus absorption and retention, or for improving the efficacy of the phytase, or for improving the subject's resistance to necrotic enteritis or for improving feed conversion ratio (FCR) or for improving weight gain in a subject or for improving feed efficiency in a subject or for modulating (e.g. improving) the immune response of the subject or for reducing populations of pathogenic bacteria in the gastrointestinal tract of a subject, or for reducing nutrient excretion in manure, which method comprising administering to a subject at least one direct fed microbial in combination with a phytase, wherein the phytase is administered to the subject at a dosage of more than about 1500 FTU/kg feed.

Feed Additive Composition

A method for improving the performance of a subject or for improving digestibility of a raw material in a feed (e.g. nutrient digestibility, such as amino acid digestibility), or for improving nitrogen retention, or for improving dietary phosphorus absorption and retention, or for improving the efficacy of the phytase, or for improving the subject's resistance to necrotic enteritis or for improving feed conversion ratio (FCR) or for improving weight gain in a subject or for improving feed efficiency in a subject or for modulating (e.g. improving) the immune response of the subject or for reducing populations of pathogenic bacteria in the gastrointestinal tract of a subject, or for reducing nutrient excretion in manure, which method comprising administering to a subject at least one direct fed microbial in combination with a phytase, wherein the phytase is administered to the subject at a dosage of more than about 1500 FTU/kg feed.

LIPASE ENZYMES

Lipase enzymes, methods of making lipase enzymes, methods of using lipase enzymes in food, feed, personal care, detergents, grain processing, pulp and paper processing, biofuels, ethanol production, textiles, dairy processing, cocoa butter processing, cocoa extraction, dietary supplements, coffee processing, coatings, water treatment, and oil processing. 1. A variant polypeptide comprising an amino acid sequence that is at least 80% identical , at least 81% , at least 82% , at least 83% , at least 84% , at least 85% , at least 86% , at least 87% , at least 88% , at least 89% , at least 90% , at least 91% , at least 92% , at least 93% , at least 94% , at least 95% , at least 96% , at least 97% , at least 98% , or at least 99% identical to the amino acid sequence of SEQ ID NO:1 , wherein the variant polypeptide has lipase activity.2. The variant polypeptide of claim 1 , comprising an amino acid residue insertion claim 1 , deletion claim 1 , or substitution to the amino acid sequence of SEQ ID NO:1 claim 1 , wherein the variant polypeptide has lipase activity.3. The variant polypeptide of claim 2 , wherein the amino acid residue insertion claim 2 , deletion claim 2 , or substitution is at the amino acid residue position number 23 claim 2 , 33 claim 2 , 82 claim 2 , 83 claim 2 , 84 claim 2 , 85 claim 2 , 160 claim 2 , 199 claim 2 , 254 claim 2 , 255 claim 2 , 256 claim 2 , 258 claim 2 , 263 claim 2 , 264 claim 2 , 265 claim 2 , 268 claim 2 , 308 claim 2 , 311 claim 2 , or any combination thereof to the amino acid sequence of SEQ ID NO:1 claim 2 , wherein the variant polypeptide has lipase activity.4. The variant polypeptide of claim 2 , wherein the amino acid substitution is selected from the group consisting of: Y23A claim 2 , K33N claim 2 , S82T claim 2 , S83D claim 2 , S83H claim 2 , S83I claim 2 , S83N claim 2 , S83R claim 2 , S83T claim 2 , S83Y claim 2 , S84S claim 2 , S84N claim 2 , 84′Y claim 2 , 84′L claim 2 , 84′S claim 2 , I85A claim 2 , I85C claim 2 , I85F ...

Method for using lipase enzymes for cleaning

A method for removing a stain from a surface using lipase enzymes, and a formulation comprising a lipase enzyme.

FEED ADDITIVE COMPOSITION

A feed additive composition comprising a direct fed microbial in combination with a protease, and a phytase, and a method for improving the performance of a subject or for improving digestibility of a raw material in a feed (e.g. nutrient digestibility, such as amino acid digestibility), or for improving nitrogen retention, or for improving the subject's resistance to necrotic enteritis or for improving feed conversion ratio (FCR) or for improving weight gain in a subject or for improving feed efficiency in a subject or for modulating (e.g. improving) the immune response of the subject or for promoting the growth of beneficial bacteria in the gastrointestinal tract of a subject, which method comprising administering to a subject a direct fed microbial in combination with a protease and a phytase. 114-. (canceled)15. A method for improving a subject's resistance to necrotic enteritis comprising administering to a subject a feed additive composition comprising a direct fed microbial (DFM) in combination with a protease and a phytase , wherein the DFM is present at a dosage of between 3.75×10CFU/g feed additive composition and 1×10CFU/g feed additive composition , the subtilisin is present at a dosage of between 1000PU/g feed additive composition and 60 ,000PU/g feed additive composition , and the 6-phytase is present at a dosage of between 200 FTU/g feed additive composition and 40 ,000 FTU/g feed additive composition ,{'i': 'Bacillus subtilis, Bacillus licheniformis, Bacillus amyloliquefaciens', 'and wherein the DFM comprises a bacterium from one or more of the following species: and combinations thereof.'}16. (canceled)17. The method according to wherein the direct fed microbial is an antipathogen direct fed microbial.1820-. (canceled)21Bacillus subtilis. The method according to wherein the direct fed microbial comprises a bacterium of one or more of the following strains: strains 3A-P4 (PTA-6506); 15A-P4 (PTA-6507); 22C-P1 (PTA-6508); 2084 (NRRL B-500130); LSSA01 ( ...

TREHALOSE HYDROGELS FOR STABILIZATION AND DELIVERY OF PROTEINS

Trehalose-based hydrogels and methods of making such hydrogels are disclosed. Specifically, a method of creating a trehalose-based hydrogel, comprising the steps of: a) forming a trehalose homopolymer or co-polymer; b) preparing a cross-linker; and c) reacting the trehalose homopolymer or co-polymer with the cross-linker to form the trehalose-based hydrogel. 1. A method of creating a trehalose-based hydrogel , comprising the steps of:a) forming a trehalose homopolymer or co-polymer;b) preparing a cross-linker; andc) reacting the trehalose homopolymer or co-polymer with the cross-linker to form the trehalose-based hydrogel.2. The method according to claim 1 , wherein the trehalose homopolymers or co-polymers have the structure of{'br': None, 'sub': 5', '1', '2', '3', '4', 'n', '6, 'R—[RRC—CRR]-R,'}{'sub': 1', '4', '1', '4, 'wherein R-Rare independently selected from hydrogen or a side chain comprising at least one carbon atom, and wherein at least one of R-Ris a side chain comprising -L-trehalose, wherein L is a linker molecule that links trehalose to the monomer through at least one of the trehalose hydroxyl groups (—OH), and'}{'sub': 5', '6', '2', '2', '2', '2', 'n', '2', '2, 'wherein Rand Rare independently selected from the group consisting of -Alkyl, -Alkenyl, -Alkynyl, -aryl, —C(CN)(Alkyl), —SC—S-Alkyl, —C(CO)(Alkyl)-(OCHCH)—COO—CHCH—CO-Alkyl (n=1-10), and biomolecules.'}3. The method according to claim 1 , wherein the trehalose homopolymers or co-polymers are either polyethylene glycols or polyethylene glycol (PEG) derivatives.4. The method according to claim 1 , wherein the cross-linker is a boronic acid-based cross-linker.6. The method according to claim 1 , wherein the trehalose homopolymer or co-polymer is a polyethylene glycol (PEG) derivative.7. The method according to claim 1 , wherein the ratio of the cross-linker to the trehalose homopolymer or co-polymer is 1:1.8. The method according to claim 1 , where the reaction between the trehalose homopolymer ...

PHYTASE HAVING IMPROVED THERMOSTABILITY

A phytase having improved thermostability is disclosed. The phytase has a modified amino acid sequence of SEQ ID NO: 2, wherein the modification is one of mutations A to D. The mutation A is to substitute amino acids at positions 143 and 262 with cysteine, the mutation B is to substitute amino acids at positions 259 and 312 with cysteine, the mutation C is to substitute amino acids at positions 205 and 257 with cysteine, and the mutation D is to substitute amino acids at positions 264 and 309 with cysteine. 1. A phytase comprising a modified amino acid sequence of SEQ ID NO: 2 , wherein the modification is one of mutations A to D , the mutation A is to substitute amino acids at positions 143 and 262 with cysteine , the mutation B is to substitute amino acids at positions 259 and 312 with cysteine , the mutation C is to substitute amino acids at positions 205 and 257 with cysteine , and the mutation D is to substitute amino acids at positions 264 and 309 with cysteine.2Escherichia coli. The phytase according to wherein a gene encoding the amino acid sequence of SEQ ID NO: 2 is isolated from and optimized.3. The phytase according to being a histidine acid phosphatase.4. The phytase according to having a full length amino acid sequence of SEQ ID NO: 24.5. The phytase according to having a full length amino acid sequence of SEQ ID NO: 26.6. The phytase according to having a full length amino acid sequence of SEQ ID NO: 28.7. The phytase according to having a full length amino acid sequence of SEQ ID NO: 30.8. A phytase comprising a modified amino acid sequence of SEQ ID NO: 2 claim 1 , wherein the modification combines mutation A and one of mutations B and D claim 1 , the mutation A is to substitute amino acids at positions 143 and 262 with cysteine claim 1 , the mutation B is to substitute amino acids at positions 259 and 312 with cysteine claim 1 , and the mutation D is to substitute amino acids at positions 264 and 309 with cysteine.9Escherichia coli. The phytase ...

FUSION OF BIOACTIVE MOLECULES

Fusion-proteins containing an enzyme, preferably a feed or food enzyme, coupled to a gut surface-binding domain are presented. The fusion-proteins can be used to promote feed utilization in animals. In a particular example, a Fusion enzyme according to the invention comprising a gut-surface-binding polypeptide segment linked to a phytase show an increased resident time in the gut, which leads to an increased amount of time given to the enzyme to catalyse the corresponding reaction which finally leads to improved feed utilisation. 1. A composition comprising a gut surface-binding polypeptide segment linked to a polypeptide or enzyme , preferably a feed or food enzyme.2. A composition according to claim 1 , wherein the gut surface-binding polypeptide segment is a bacterial collagen-binding polypeptide segment.3. A composition according to claim 1 , wherein the gut surface-binding polypeptide segment is a segment of a mucus-binding protein4. The composition of claim 1 , wherein the enzyme is a phytase.5. The composition of claim 1 , wherein the gut surface-binding polypeptide segment and the enzyme are portions of a fusion-protein.6. An isolated nucleic acid sequence comprising a nucleic acid sequence which encodes the composition of .7. A nucleic acid construct comprising the nucleic acid sequence of operably linked to one or more control sequences that direct the production of the fusion-protein in a suitable expression host.8. A recombinant expression vector comprising the nucleic acid construct of .9. A recombinant host cell comprising the nucleic acid construct of .10. A method for producing the composition or fusion-protein comprising a gut surface-binding polypeptide segment linked to a polypeptide or enzyme claim 9 , preferably a feed or food enzyme claim 9 , the method comprising (a) cultivating the host cell of to produce a supernatant comprising the fusion protein and (b) recovering the fusion-protein.11. An animal feed composition comprising at least one ...

FEED ADDITIVE COMPOSITION

A feed additive composition comprising a direct fed microbial (DFM) in combination with a phytase derivable from spp. and a method for improving the performance of a subject or for improving digestibility of a raw material in a feed (e.g. nutrient digestibility, such as amino acid digestibility), or for improving nitrogen retention, or for avoiding the negative effects of necrotic enteritis or for improving feed conversion ratio (FCR) or for improving weight gain in a subject or for improving feed efficiency in a subject or for modulating (e.g. improving) the immune response of the subject, or for promoting the growth of beneficial bacteria in the gastrointestinal tract of a subject or for reducing populations of pathogenic bacteria in the gastrointestinal tract of a subject, or for reducing nutrient excretion in manure, which method comprising administering to a subject a direct fed microbial (DFM) in combination with a phytase derivable from spp. 1Citrobacter. A feed additive composition comprising a direct fed microbial (DFM) in combination with a phytase derivable from spp.2CitrobacterCitrobacter braakii, Citrobacter freundii, Citrobacter amalonaticus, Citrobacter gillenii, Citrobacter intermedius, Citrobacter koseri, Citrobacter murliniae, Citrobacter rodentium, Citrobacter sedlakii, Citrobacter werkmaniiCitrobacter youngae.. A feed additive composition according to wherein the phytase is derivable from a bacterium selected from the group consisting of: and3Citrobacter braakii.. A feed additive composition according to wherein the phytase is derived from46-. (canceled)7. A feed additive composition according to wherein the phytase comprises a polypeptide comprising an amino acid sequence which has at least 99.1% identity with amino acids 23-433 of SEQ ID No 1 or 2.820-. (canceled)21Citrobacter. A method for improving the performance of a subject or for improving digestibility of a raw material in a feed (e.g. nutrient digestibility claim 1 , such as amino acid ...

Animal Feed Compositions and Uses Thereof

The present invention relates to animal feed compositions comprising polypeptides having lysozyme activity and polypeptides having phytase activity and uses thereof. 1. An animal feed or animal feed additive comprising one or more polypeptides having phytase activity and one or more polypeptides having lysozyme activity , wherein:(a) the polypeptide having lysozyme activity is from glycosyl hydrolyase family 25 and is obtained or obtainable from the kingdom Fungi; and(b) the polypeptide having phytase activity is classified as an EC 3.1.3.26 phytase (4-phytase).2. The animal feed or animal feed additive of claim 1 , wherein the animal feed or animal feed additive improves the European Production Efficiency Factor (EPEF) of an animal by at least 1%.3. The animal feed or animal feed additive of claim 1 , animal feed or animal feed additive improves the Feed Conversion Ratio (FCR) of an animal by at least 1%.4Faecalibacterium. The animal feed or animal feed additive of claim 1 , wherein the animal feed or animal feed additive increases the bacteria of genus in the microbiota of the GI tract of an animal.5Faecalibacterium. The animal feed or animal feed additive of claim 4 , wherein the bacteria of genus comprise 16S rRNA that has at least 90% sequence identity to SEQ ID NO: 33.6. The animal feed or animal feed additive of claim 1 , wherein the polypeptide having lysozyme activity is selected from the group consisting of:(a) a polypeptide having at least 50% sequence identity to SEQ ID NO: 27;(b) a polypeptide having at least 50% sequence identity to SEQ ID NO: 29;(c) a variant of SEQ ID NO: 27 comprising one or more amino acid substitutions, and/or one or more amino acid deletions, and/or one or more amino acid insertions or any combination thereof in 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49 or 50 positions; and(d) a variant ...

Plug for oil field service work and method of production

Plugs for use in hydrocarbon recovery operations having lower and upper anchor slip assemblies with individual anchors, upper and lower elements, a shoe and tubular mandrel.

Processes for increasing extraction of enzymes from animal feed and measuring activity of the same

Methods of increasing extraction of enzymes from animal feed and measuring enzyme activity are described herein.

METHODS OF USING THERMOSTABLE SERINE PROTEASES

Methods of using thermostable serine proteases are described herein.

PRODUCTION OF PHYTATE DEGRADING ENZYMES IN $i(TRICHODERMA)

A highly efficient overexpression system for phytase and pH 2.5 acid phosphatase in Trichoderma is described. This system results in enzyme compositions that are especially useful in the animal feed industry.

A process for steeping cereals with a new enzyme preparation

A method for improving the solubility of vegetable proteins

The present invention relates to a method for improving the solubility of vegetable proteins. More specifically, the invention relates to methods for the solubilization of proteins in vegetable protein sources, which methods comprise treating the vegetable protein source with an efficient amount of one or more phytase enzymes, and treating the vegetable protein source with an efficient amount of one or more proteolytic enzymes. In another aspect, the invention provides animal feed additives comprising a phytase and one or more proteolytic enzymes.

Process for steeping cereals with a new enzyme preparation

Corn or sorghum kernels are steeped in warm water containing sulfur dioxide in the presence of an enzyme preparation including one or more phytin-degrading enzymes, such as phytase and acid phosphatases, to eliminate or greatly reduce phytic acid and the salts of phytic acid.

METHOD FOR SOAKING CEREALS WITH A NEW ENZYME PREPARATION.

Method for damping of maize and sorghum grains

Использование: изобретение относитс  к переработке зерна, точнее к способу замачивани  зерна кукурузыоИли сорго. Сущность: удаление фитиновой кислоты осуществл ют выдерживанием при темпе ратуре 2Q-60°C в присутствии диоксида серы и по меньшей мере одного фитин-декструктурирующего фермента до разрушени  основной части фитиновой кислоты . Ферментный препарат включает фи- тазу и/или кислотную фосфатазу. Зерна кукурузы или сорго замачивают в течение 12-48 ч. 5з.п. ф-лы.

Methods of using thermostable serine proteases.

En el presente documento se describen métodos para usar serina proteasas termoestables.

METHOD OF APPLICATION OF LIPASE ENZYMES FOR CLEANING

РОССИЙСКАЯ ФЕДЕРАЦИЯ (19) RU (11) (13) 2019 140 871 A (51) МПК C07K 14/37 (2006.01) ФЕДЕРАЛЬНАЯ СЛУЖБА ПО ИНТЕЛЛЕКТУАЛЬНОЙ СОБСТВЕННОСТИ (12) ЗАЯВКА НА ИЗОБРЕТЕНИЕ (21)(22) Заявка: 2019140871, 10.05.2018 (71) Заявитель(и): БАСФ СЕ (DE) Приоритет(ы): (30) Конвенционный приоритет: 12.05.2017 US 62/505,500 (85) Дата начала рассмотрения заявки PCT на национальной фазе: 12.12.2019 R U (43) Дата публикации заявки: 16.06.2021 Бюл. № 17 (72) Автор(ы): ПОП, Кристина (US), ЭСПЕР, Клаудиа (DE), ШПАНГЕНБЕРГ, Оливер (DE), КЛАЙН, Кэти (US) (86) Заявка PCT: (87) Публикация заявки PCT: WO 2018/209026 (15.11.2018) A Адрес для переписки: 105064, г. Москва, а/я 88, ООО "Патентные поверенные Квашнин, Сапельников и партнеры", Квашнин Валерий Павлович R U (57) Формула изобретения 1. Моющий состав, содержащий: по меньшей мере один полипептид согласно аминокислотной последовательности SEQ ID NO: 1, или по меньшей мере один полипептид на по меньшей мере 80% идентичный аминокислотной последовательности SEQ ID NO: 1, имеющий липазную активность, и по меньшей мере один моющий компонент. 2. Моющий состав по п. 1, где полипептид содержит по меньшей мере одну вставку, делецию или замещение аминокислотного остатка в положении аминокислотного остатка номер: 23, 33, 82, 83, 84, 85, 160, 199, 254, 255, 256, 258, 263, 264, 265, 268, 308, 311, или любой их комбинации, относительно аминокислотной последовательности SEQ ID NO: 1, и полипептид имеет липазную активность. 3. Моющий состав по п. 2, где по меньшей мере одно аминокислотное замещение выбрано из группы, состоящей из: Y23A, K33N, S82T, S83D, S83H, S83I, S83N, S83R, S83T, S83Y, S84S, S84N, 84'Y, 84'L, 84'S, I85A, I85C, I85F, I85H, I85L, I85M, I85P, I85S, I85T, I85V, I85Y, K160N, P199I, P199V, I254A, I254C, I254E, I254F, I254G, I254L, I254M, I254N, I254R, I254S, I2454V, I254W, I254Y, I255A, I255L, A256D, L258A, L258D; L258E, L258G, L258H, L258N, L258Q, L258R, L258S, L258T, L258V, D263G, D263K, D263P, D263R, D263S; T264A, T264D, T264G, T264I, T264L ...

Polypeptide having phytase activity and nucleic acid encoding the same

Nucleic acids of aspergillus fumigatus encoding industrial enzymes and methods of use

The present invention provides nucleotide sequences of Aspegillus fumigatus that encode proteins which exhibit enzyme activities. Vectors, expression constructs, and host cells comprising the nucleotide sequences of the enzyme genes are also provided. The invention further provides methods for producing the enzymes, and methods for modifying the enzymes in order to improve their desirable characteristics. The activities displayed by the enzymes of the invention include those of a tannase, cellulase, glucose oxidase, glucoamylase, phytase, β-galactosidases, invertase, lipase, α-amylase, laccase, polygalacturonase or xylanase. The enzymes of the invention can be used in a variety of industrial processes. Enzymatically active compositions in various forms as well as antibodies to the enzymes and fragments thereof, are also provided.

Methods of using thermostable serine proteases

Methods of using thermostable serine proteases are described herein.

Production of enzymes in seeds and their use

A method of catalyzing in vitro reactions using seeds containing enhanced amounts of enzymes is disclosed. The method involves adding transgenic, non-wild type seeds, preferably in a ground form, to a reaction mixture, especially a bakery process and allowing the enzymes in the seeds to increase the rate of reaction. By directly adding the seeds to the reaction mixture the method provides a solution to the expensive and problematic process of extracting and purifying the enzyme. Methods of treatment are also provided whereby a subject lacking a sufficient supply of an enzyme is administered the enzyme in the form of seeds containing enhanced amounts of the enzyme.

Expression of phytase in plants

The present invention provides for the expression of phytase in transgenic plants or plant organs and methods for the production of such plants. DNA expression constructs are provided for the transformation of plants with a gene encoding phytase under the control of regulatory sequences which are capable of directing the expression of phytase. These regulatory sequences include sequences capable of directing transcription in plants, either constitutively, or stage and/or tissue specific, depending on the use of the plant or parts thereof. The transgenic plants and plant organs provided by the present invention may be applied to a variety of industrial processes either directly, e.g. in animal feeds or alternatively, the expressed phytase may be extracted and if desired, purified before application.

Production of enzymes in seeds and their use

A method of catalyzing in vitro reactions using seeds containing enhanced amounts of enzymes is disclosed. The method involves adding transgenic, non-wild type seeds, preferably in a ground form, to a reaction mixture and allowing the enzymes in the seeds to increase the rate of reaction. By directly adding the seeds to the reaction mixture the method provides a solution to the expensive and problematic process of extracting and purifying the enzyme. Methods of treatment are also provided whereby a subject lacking a sufficient supply of an enzyme is administered the enzyme in the form of seeds containing enhanced amounts of the enzyme.

Phytases, nucleic acids that encode them and methods for their production and use

Un ácido nucleico aislado, sintético o recombinante que comprende (a) una secuencia de ácido nucleico que codifica un polipéptido que tiene una actividad fitasa y que tiene al menos 95%, 96%, 97%, 98% o 99% o más identidad de secuencia con SEC ID Nº: 1, y que tiene una modificación de nucleótidos donde los nucleótidos en las posiciones 1045 a 1047 de SEC ID Nº: 1 se cambian a TAT O TAC, donde el polipéptido tiene termoestabilidad mejorada sobre la fitasa de SEC ID Nº: 2; (b) un ácido nucleico que codifica un polipéptido que tiene una actividad fitasa y que tiene al menos 95%, 96%, 97%, 98% o 99% o más identidad de secuencia de SEC ID Nº: 2, y que tiene una modificación de aminoácidos donde el aminoácido treonina en la posición de aminoácido 349 de SEC ID Nº: 2 se reemplaza por una tirosina, donde el polipéptido tiene termoestabilidad mejorada sobre la fitasa de SEC ID Nº: 2; (c) la secuencia de ácido nucleico de (a) o (b) que codifica un polipéptido que tiene una actividad fitasa pero que carece de: una secuencia señal o secuencia de proproteína, o una secuencia promotora homóloga; (d) el ácido nucleico de cualquiera de (a) a (c) que codifica un polipéptido que tiene una actividad fitasa y que comprende además una secuencia de aminoácidos heteróloga, o el ácido nucleico de cualquiera de (a) a (c) que comprende además una secuencia de nucleótidos heteróloga; (e) el ácido nucleico de (d), donde la secuencia de aminoácidos heteróloga comprende, o consiste en una secuencia que codifica una secuencia señal heteróloga (líder), o un marcador o un epítopo, o la secuencia de nucleótidos heteróloga que comprende un promotor heterólogo o codifica una secuencia diana; o (f) una secuencia de ácido nucleico completamente complementaria de la secuencia de ácido nucleico de cualquiera de (a) a (e). An isolated, synthetic or recombinant nucleic acid comprising (a) a nucleic acid sequence encoding a polypeptide having a phytase activity and having at least 95%, 96%, 97%, 98% or 99% ...

Variant Buttiauxella sp. phytases having altered properties

The present invention relates to variant phytase enzymes having altered properties.

New strain bacillus sp.ds11 (kctc 0231bp)and new phytase produced from this

본 발명은 신균주 바실러스속(Bacillus sp.) DS11(KCTC 0231BP)과 이로부터 생산되는 신규 파이타아제(Phytase)에 관한 것으로서, 더욱 상세하게는 신균주 바실러스속(Bacillus sp.) DS11과 단위가축에 급여하는 곡류내 인(P)의 체내 이용성을 높여주는 신규한 파이타아제 효소에 관한 것이다. The present invention relates to Bacillus sp. DS11 (KCTC 0231BP) and a novel phytase produced therefrom. More specifically, Bacillus sp. DS11 and a unit livestock The present invention relates to a novel phytase enzyme that increases the availability of phosphorus (P) in the body to be fed.

A process for steeping cereals with a new enzyme preparation

내용없음. None.

Method for improving the nutritional value of animal feed

本发明涉及至少一种细菌植酸酶与一种或多种蛋白酶相组合在动物饲料中用于改进体重增加和/或饲料转化率(FCR)的用途，其中该植酸酶按以下量(剂量范围)中的一种或多种施用：1’000FYT/kg饲料、2’000FYT/kg饲料、3’000FYT/kg饲料，并且其中该蛋白酶按以下量(剂量范围)中的一种施用：10’000单位/kg饲料、11’000、12’000、13’000、14’000、15’000、16’000、17’000、18’000、19’000、20’000单位/kg饲料。

METHOD FOR CONVERTING PHYTATE IN INORGANIC PHOSPHATE

Methods for application of thermostable serine proteases

FIELD: biotechnology. SUBSTANCE: method for producing fermentation products from a starch-containing material, involving: (a) liquefaction of the starch-containing material by means of a mixture of enzymes, containing 1 to 20 g serine thermostable protease/metric ton (MT) of the starch-containing material, characterised by at least 80% identity of the sequence with SEQ ID NO: 3; (b) saccharification of the product from stage (a); (c) fermentation by means of a suitable organism, and (d) optionally isolation of the product produced at stage (C). EFFECT: invention allows for use of the protease in unfavourable conditions while preserving or improving the activity thereof. 4 cl, 8 dwg, 13 tbl, 14 ex РОССИЙСКАЯ ФЕДЕРАЦИЯ (19) RU (11) (13) 2 771 261 C2 (51) МПК C12N 9/50 (2006.01) C12N 15/57 (2006.01) C12P 7/06 (2006.01) C12P 21/02 (2006.01) A23J 3/34 (2006.01) ФЕДЕРАЛЬНАЯ СЛУЖБА ПО ИНТЕЛЛЕКТУАЛЬНОЙ СОБСТВЕННОСТИ (12) ОПИСАНИЕ ИЗОБРЕТЕНИЯ К ПАТЕНТУ (52) СПК C12N 9/50 (2022.02); C12P 7/06 (2022.02); A23J 3/34 (2022.02) (21)(22) Заявка: 2019122835, 18.12.2017 (24) Дата начала отсчета срока действия патента: Дата регистрации: 29.04.2022 21.12.2016 US 62/437,340 (43) Дата публикации заявки: 25.01.2021 Бюл. № 3 (45) Опубликовано: 29.04.2022 Бюл. № 13 (56) Список документов, цитированных в отчете о поиске: WO 2014209789 A1, 31.12.2014. WO 2016087427 A1, 09.06.2016. WO 2016069552 A2, 06.05.2016. RU 2558261 C2, 27.07.2015. RU 2566549 C2, 27.10.2015. (85) Дата начала рассмотрения заявки PCT на национальной фазе: 22.07.2019 (86) Заявка PCT: US 2017/067112 (18.12.2017) C 2 C 2 (73) Патентообладатель(и): ДЮПОН НЬЮТРИШН БАЙОСАЙЕНСИЗ АПС (DK) (87) Публикация заявки PCT: 2 7 7 1 2 6 1 WO 2018/118815 (28.06.2018) R U 2 7 7 1 2 6 1 Приоритет(ы): (30) Конвенционный приоритет: R U 18.12.2017 (72) Автор(ы): КРАМЕР, Якоб Флювхольм (DK), КОЛКМЭН, Марк Антон Бернхард (US), МА, Чжэнь (US), СХЕФФЕРС, Мартейн (NL), ШИПОВСКОВ, Степан (DK), ВАН БРЮССЕЛ-ЗВЕЙНЕН, Марко (NL), ЮЙ, Шукунь (SE) Адрес для ...

Patent RU2019140871A3

ВУ’ ” 2019140871`” АЗ Дата публикации: 09.09.2021 Форма № 18 ИЗПМ-2011 Федеральная служба по интеллектуальной собственности Федеральное государственное бюджетное учреждение ж 5 «Федеральный институт промышленной собственности» (ФИПС) ОТЧЕТ О ПОИСКЕ 1. . ИДЕНТИФИКАЦИЯ ЗАЯВКИ Регистрационный номер Дата подачи 201914087 1/10(079806) 10.05.2018 РСТ/О$2018/031969 10.05.2018 Приоритет установлен по дате: [ ] подачи заявки [ ] поступления дополнительных материалов от к ранее поданной заявке № [ ] приоритета по первоначальной заявке № из которой данная заявка выделена [ ] подачи первоначальной заявки № из которой данная заявка выделена [ ] подачи ранее поданной заявки № [Х] подачи первой(ых) заявки(ок) в государстве-участнике Парижской конвенции (31) Номер первой(ых) заявки(ок) (32) Дата подачи первой(ых) заявки(ок) (33) Код страны 1. 62/505,500 12.05.2017 05 Название изобретения (полезной модели): [Х] - как заявлено; [ ] - уточненное (см. Примечания) СПОСОБ ПРИМЕНЕНИЯ ЛИПАЗНЫХ ФЕРМЕНТОВ ДЛЯ ОЧИСТКИ Заявитель: БАСФ СЕ, РЕ 2. ЕДИНСТВО ИЗОБРЕТЕНИЯ [Х] соблюдено [ ] не соблюдено. Пояснения: см. Примечания 3. ФОРМУЛА ИЗОБРЕТЕНИЯ: [Х] приняты во внимание все пункты см. п см. Примечания [ ] приняты во внимание следующие пункты: р [ ] принята во внимание измененная формула изобретения (см. Примечания) 4. КЛАССИФИКАЦИЯ ОБЪЕКТА ИЗОБРЕТЕНИЯ (ПОЛЕЗНОЙ МОДЕЛИ) (Указываются индексы МПК и индикатор текущей версии) С07К 14/37 (2006.01) 5. ОБЛАСТЬ ПОИСКА 5.1 Проверенный минимум документации РСТ (указывается индексами МПК) 5.2 Другая проверенная документация в той мере, в какой она включена в поисковые подборки: 5.3 Электронные базы данных, использованные при поиске (название базы, и если, возможно, поисковые термины): Е-Габгагу, Езрасепе, РаЗеагсь, РАТЕМТСОРЕ, КОРТО, МСВТ, ЕМВГ-ЕВ1, Соозе, Соозе эсБо][аг, РиБМеа, ОЗРТО, Заепсе еси 6. ДОКУМЕНТЫ, ОТНОСЯЩИЕСЯ К ПРЕДМЕТУ ПОИСКА Кате- Наименование документа с указанием (где необходимо) частей, Относится к гория* относящихся к предмету поиска ...

ENZYMAN GRANULATE I CONTAINING PHYTASE

1. Ферментный гранулят, содержащий фитазу, для корма, частицы которого содержат ! A) ядро, содержащее по меньшей мере одну фитазу, и по меньшей мере один твердый носитель, пригодный для корма, и ! B) одно покрытие, расположенное на ядре, прчем ядра частиц или все частицы при суспендировании или растворении в полностью опресненной воде при температуре 25°С дают в итоге значение рН в интервале от 4,5 до 6,5. ! 2. Ферментный гранулят по п.1, в котором ядро для установления значения рН содержит по меньшей мере один буфер. ! 3. Ферментный гранулят по п.1 или 2, причем по меньшей мере одно покрытие, расположенное на поверхности ядра, до по меньшей мере 70% состоит из веществ, выбираемых из восков, насыщенных жирных кислот, сложных эфиров насыщенных жирных кислот, полиолефинов, производных целлюлозы, поливинилацетата, поливинилпирролидона, поливинилового спирта, сополимеров винилацетат/винилпирролидон, полиалкиленгликолей и их смесей. ! 4. Ферментный гранулят по п.1 или 2, причем по меньшей мере одно покрытие, расположенное на поверхности ядра, до по меньшей мере 90% состоит из гидрофобных веществ, нерастворимых в воде. ! 5. Ферментный гранулят по п.4, причем гидрофобное вещество выбирают из одного или нескольких триглицеридов. ! 6. Ферментный гранулят по п.1 или 2, причем массовое соотношение ядра к покрытию находится в интервале от 70:30 до 99:1. ! 7. Ферментный гранулят по п.1 или 2, частицы которого имеют средний размер частиц в интервале от 100 до 2000 мкм. ! 8. Ферментный гранулят по п.1 или 2, при этом ферментное ядро получают при применении водного ферментного концентрата, который при температуре 25°С имеет значение рН в интервале от 4,5 до 6,5. ! 9. Ферментный г� РОССИЙСКАЯ ФЕДЕРАЦИЯ (19) RU (11) 2008 113 843 (13) A (51) МПК A23K 1/165 (2006.01) ФЕДЕРАЛЬНАЯ СЛУЖБА ПО ИНТЕЛЛЕКТУАЛЬНОЙ СОБСТВЕННОСТИ, ПАТЕНТАМ И ТОВАРНЫМ ЗНАКАМ (12) ЗАЯВКА НА ИЗОБРЕТЕНИЕ (21), (22) Заявка: 2008113843/13, 11.09.2006 (71) Заявитель(и): БАСФ СЕ (DE) (30) Конвенционный приоритет: 12.09. ...

High expression cereal phytase gene

The present invention provides mutant cereal plants and mature grain thereof, characterised by enhanced levels of the enzyme phytase in the grain, and methods for inducing, detecting and selecting the mutant cereal plants. The invention further relates to animal feed comprising said grain having enhanced amounts of phytase.

Phytases, nucleic acids encoding them and methods for making and using them

Process for making instant dehydrated legume puree.

Process for producing an instant dried dehydrated legume puree

L'invention concerne un procédé de fabrication d'une purée de légumineuses déshydratées instantanée, caractérisé en ce qu'il comprend les étapes suivantes: a) broyage de graines de légumineuses en particules et tamisage éventuel de celles-ci; b) ajout aux particules résultantes d'un émulsifiant; c) cuisson-extrusion du mélange de particules et d'émulsifiant obtenu à l'étape (b), après addition d'eau, dans une extrudeuse à deux vis chauffée; d) broyage du produit cuit extrudé obtenu à l'étape c) en particules et tamisage éventuel de celles-ci; et e) facultativement incorporation aux particules obtenues en d) d'additifs alimentaires et/ou épices. Utilisation par l'industrie alimentaire. The invention relates to a process for the manufacture of an instant dehydrated legume puree, characterized in that it comprises the following steps: a) crushing legume seeds into particles and optionally sieving them; b) adding to the resulting particles an emulsifier; c) cooking-extrusion of the mixture of particles and emulsifier obtained in step (b), after addition of water, in a heated twin-screw extruder; d) grinding of the cooked extruded product obtained in step c) into particles and possible sieving thereof; and e) optionally incorporation into the particles obtained in d) of food additives and / or spices. Use by the food industry.

METHOD TO INCREASE WEIGHT GAIN AND/OR IMPROVE THE RATIO OF COVERSION OF FARM ANIMAL FOODS

MÉTODO PARA AUMENTAR O GANHO DE PESO E/OU MELHORAR A RAZÃO DE CORVERSÃO DE ALIMENTOS DE ANIMAIS DE FAZENDA, E, USO DE UMA OU MAIS ENZIMAS PROTOLÍTICAS EM COMBINAÇÃO COM PELO MENOS UMA FITASE NO ALIMENTO PARA ANIMAIS. A presente invenção refere-se ao uso de pelo menos uma fitase bacteriana em combinação com uma ou mais proteases no alimento para animais para melhorar o ganho de peso e/ou a Razão de Conversão de Alimento (FCR), em que a fitase é administrada em uma ou mais dentre as seguintes quantidades (faixas de dosagem): 1.000 FYT/kg de alimento, 2.000 FYT/kg de alimento, 3.000 FYT/kg de alimento e em que a protease é administrada em uma das seguintes quantidades (faixas de dosagem): 10.000 unidades/kg de alimento, 11.000, 12.000, 13.000, 14.000, 15.000, 16.000, 17.000, 18.000, 19.000, 20.000 unidades/kg de alimento. METHOD FOR INCREASE WEIGHT GAIN AND/OR IMPROVE THE RATIO OF CORVERSION OF FARM FOOD, AND, USE OF ONE OR MORE PROTOLYTIC ENZYMES IN COMBINATION WITH AT LEAST ONE PHYTASE IN FOOD. The present invention relates to the use of at least one bacterial phytase in combination with one or more proteases in animal feed to improve weight gain and/or Feed Conversion Ratio (FCR), wherein the phytase is administered in one or more of the following amounts (dosage ranges): 1000 FYT/kg feed, 2000 FYT/kg feed, 3000 FYT/kg feed and where the protease is administered in one of the following amounts (dosage ranges) ): 10,000 units/kg of food, 11,000, 12,000, 13,000, 14,000, 15,000, 16,000, 17,000, 18,000, 19,000, 20,000 units/kg of food.

Hafnia phytase

本发明涉及具有肌醇六磷酸酶活性的分离的多肽和编码所述多肽的分离的多核苷酸。所述多肽与源自蜂房哈夫尼菌的肌醇六磷酸酶相关，所述源自蜂房哈夫尼菌的肌醇六磷酸酶的氨基酸序列在所附的序列表中显示为SEQ ID NO：10。本发明还涉及包含所述多核苷酸的核酸构建体、载体和宿主细胞，以及生产和使用所述多肽的方法，特别是在动物饲料中。

Recombinant bacterial phytases and uses thereof

A purified and modified phytase enzyme from Escherichia coli K12 appA phytas e is provided. The enzyme has phytase activity and improved thermal tolerance as compared with the wild-type enzyme. In addition, the enzyme has improved protease stability at low pH. Glycosylation of the modified phytase provided a further improved enzyme having improved thermal tolerance and protease stability. The enzyme can be produced from native or recombinant host cells and can be used to aid in the digestion of phytase where desired. In particular, the phytase of the present invention can be used in foodstuffs t o improve the feeding value of phytase rich ingredients.

Peniophora phytase

The present invention relates to an isolated polypeptide exhibiting phytase activity, the corresponding cloned DNA sequences, a process for preparing the polypeptide, and the use thereof for a number of industrial applications, in particular in animal feed. The novel phytase is derived from Peniophora lycii and has some interesting features, such as high initial affinity for the 6-position of phytic acid, a high initial rate of liberating phosphate from phytic acid and an exceptionally high specific activity.

Feed additive composition

Expression of phytase in aspergillus niger

FEED COMPOSITIONS

COMPOSIÇÕES DE RAÇÃO. A presente invenção refere-se a polipeptídeos de clado Tm-fitase alta robustos geneticamente modificados e fragmentos dos mesmos. São também descritos métodos para produzir tal clado Tm-fitase alta e fragmentos do mesmo e uso dos mesmos para melhorar o desempenho do animal. FEED COMPOSITIONS. The present invention relates to genetically engineered robust high Tm-phytase clade polypeptides and fragments thereof. Also described are methods for producing such a high Tm-phytase clade and fragments thereof and their use to improve animal performance.

Recombinant bacterial phytases and uses thereof

A purified recombinant phytase enzyme derived from <i>Escherichia coli</i> B. The enzyme has a molecular weight of about 47.1 kilodaltons and has phytase activity. The enzyme can be produced from native or recombinant host cells and can be used to aid in the digestion of phytate where desired. In particular, the phytase of the present invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients.

Phytases, nucleic acids encoding them and methods of making and using them

本发明的发明名称是肌醇六磷酸酶、编码它们的核酸及制备和使用它们的方法。本发明涉及肌醇六磷酸酶、编码它们的多核苷酸、本发明的多核苷酸和多肽的应用、以及这类多核苷酸和多肽的产生和分离。特别是，本发明提供了在高温条件下具有肌醇六磷酸酶活性的多肽，和暴露于高温后保持活性的肌醇六磷酸酶。本发明的肌醇六磷酸酶除了在更高的温度下，在低温下也可以是耐热的和/或热稳定的。本发明的肌醇六磷酸酶可被用在食品中以改善富肌醇六磷酸成分的饲养价值。本发明的肌醇六磷酸酶可被配制为例如帮助肌醇六磷酸消化的食物或饲料或补充剂。本发明的食物和饲料可以是粒料、液体、粉末等形式。在一个方面，本发明的肌醇六磷酸酶在粒化期间对热变性稳定；并且这减少肌醇六磷酸酶产品的成本，同时保持体内功效和在饲料中检测到活性。

Phytases, nucleic acids encoding them and method for making and using them

This invention relates to phytases, polynucleotides encoding them, uses of the poiynucleotides and polypeptides of the invention, as well as the production and isolation of such polynucleotides and polypeptides. In particular, the invention provides polypeptides having phytase activity under high temperature conditions, and phytases that retain activity after exposure to high temperatures. The phytases of the invention can be thermotolerant and/or thermostable at low temperatures, in addition to higher temperatures. The phytases of the invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients. The phytases of the invention can be formulated as foods or feeds or supplements for either to, e.g., aid in the digestion of phytate. The foods or feeds of the invention can be in the form of pellets, liquids, powders and the like. In one aspect, phytases of the invention are stabile against thermal denaturation during pelleting; and this decreases the cost of the phytase product while maintaining in vivo efficacy and detection of activity in feed. (Figure 6)

Novel phytase

(57)【要約】 本発明は、大腸菌Ｂに由来する精製されたフィチン酸酵素を提供する。この酵素は、分子量が約４７．１キロダルトンであり、フィターゼ活性をもっている（配列番号２）。この酵素は天然または組換え宿主細胞で産生させることができ、所望によりフィチン酸の消化の補助として使用することができる。特に、本発明のフィターゼは動物の飼料中に用いることができる。

Production of enhanced levels of enzymes in the seeds of transgenic plants and the use of these seeds

Using mutations to improve aspergillus phytases

The present invention relates to an isolated nucleic acid molecule encoding a mutant phytase and the isolated mutant phytase itself. The present invention further relates to methods of using the isolated nucleic acid molecule and the isolated mutant phytase of the present invention.

MODIFIED FITASES

Настоящее изобретение раскрывает термостабильную фитазу, полученную путем введения по меньшей мере одной дисульфидной связи в последовательность аминокислот фитазы Escherichia coli (кишечной палочки) дикого либо мутированного типа, в результате чего улучшаются характеристики фитазы, в частности ее термостабильность, стабильность при выпаривании и гранулировании по сравнению с существующей фитазой дикого либо мутированного типа; ее термостабильность значительно превышает в том числе и термостабильность существующих на сегодняшний день модифицированных образцов с введенной дисульфидной связью. The present invention discloses a thermostable phytase obtained by introducing at least one disulfide bond into the amino acid sequence of wild or mutated type Escherichia coli phytase, as a result of which the characteristics of the phytase, in particular its thermostability, evaporation and granulation stability, are improved as compared to existing phytase of wild or mutated type; its thermal stability significantly exceeds the thermal stability of existing modified samples with introduced disulfide bonds.

Hafnia Phytase

The present invention relates to isolated polypeptides having phytase activity and isolated polynucleotides encoding the polypeptides. The polypeptides are related to a phytase derived from Hafnia alvei , the amino acid sequence of which is shown in the appended sequence listing as SEQ ID NO: 10. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides, in particular within animal feed.

Phytate hydrolysis and enzyme composition for hydrolizing phytate

An enzyme composition having a synergetic phytate hydrolyzing activity comprising a phytase having phytate hydrolyzing activity at a pH of from 2.5 to 5.0 and an acid phosphatase having phytate hydrolyzing activity at a pH of 2.5, in a low ratio corresponding to a pH 2.5/5.0 activity profile of from 0.8/1.0 to 3/1. Said enzyme composition preferably displays a higher synergetic phytate hydrolyzing efficiency through thermal treatment. Fungal enzymes, especially those from Aspergillus, are preferred. Use of said enzyme composition in food, feed and fodder products to improve phytate hydrolysis.

METHOD FOR PRODUCING SOLID ENZYME FEED GRANULATORS

1. Способ получения твердых ферментных гранулятов для корма, включающий следующие стадии: ! a) экструдирование пасты, содержащей ферменты, которая наряду с водой содержит ! i) от 50 до 96,9 мас.%, по меньшей мере, одного твердого носителя, подходящего для корма, ! ii) от 0,1 до 20 мас.%, по меньшей мере, одного полимерного связующего средства, растворимого в воде, ! iii) от 3 до 49,9 мас.%, по меньшей мере, одного фермента, при этом массовые части i), ii) и iii) являются соответственно в расчете на не водные составляющие пасты; ! b) изготовление экструдата до получения сырого гранулята с содержанием воды не больше чем 15 мас.%, и ! c) покрытие сырого гранулята гидрофобным материалом, который до, по меньшей мере, 70 мас.%, в расчете на гидрофобный материал, выбирают из насыщенных жирных кислот, сложных эфиров насыщенных жирных кислот и их смесей. ! 2. Способ по п.1, при этом на стадии с) сырой гранулят покрывают гидрофобным материалом в количестве от 1 до 30 мас.%, в расчете на не водные составляющие сырого гранулята. ! 3. Способ по п.1 или 2, при этом на стадии с) гидрофобный материал применяют в форме его расплава. ! 4. Способ по п.1 или 2, при этом при этом гидрофобный материал до, по меньшей мере, 70 мас.% состоит из одного или нескольких триглицеридов. ! 5. Способ по п.1 или 2, при этом на стадии b) экструдат из стадии а) подвергают сферонизации и затем сушат до получения сырого гранулята с остаточным содержанием воды не более чем 15 мас.%, в расчете на общую массу сырого гранулята. ! 6. Способ по п.1 или 2, при этом сырой гранулят, полученный на стадии b), имеет средний размер частиц в интервале от 100 до 2000 мкм. ! 7. Способ по п.1 или 2, при этом носитель включает, по меньшей мере, один поли� РОССИЙСКАЯ ФЕДЕРАЦИЯ (19) RU (11) 2008 113 850 (13) A (51) МПК A23K C12N C12N C12N C12N 1/165 11/10 11/12 9/14 9/98 (2006.01) (2006.01) (2006.01) (2006.01) (2006.01) ФЕДЕРАЛЬНАЯ СЛУЖБА ПО ИНТЕЛЛЕКТУАЛЬНОЙ СОБСТВЕННОСТИ, ПАТЕНТАМ И ТОВАРНЫМ ЗНАКАМ (12) ЗАЯВКА НА ...

Fermentation method and uses thereof

Various examples according to the present disclosure provide a fermentation method. The fermentation method includes producing at least about 10 g/L of a bioproduct and one or more heterologous polypeptides by fermenting a medium using an engineered microorganism. About 2 wt% to about 100 wt% of the one or more heterologous polypeptides are encapsulated intercellularly in the engineered microorganism. The method further includes isolating the engineered microorganism including the encapsulated one or more heterologous polypeptides. About 50 wt% to about 100 wt% of the one or more heterologous polypeptides retain functionality following isolation of the engineered microorganism.

Process for microbial production of a valuable compound

The present invention relates to a process for the production of a valuable compound by cultivation of a microorganism comprising cultivating the microorganism in a medium wherein all nutrients are provided in excess over the whole cultivation period and wherein a suitable amount of oxygen is fed to the culture to maintain the culture under conditions of oxygen limitation.

Methods for liberating phosphorus from organic matter

La presente invención proporciona composiciones basadas en microbios que comprenden levaduras biológicamente puras, y/o uno o más subproductos de crecimiento microbiano, tal como enzima. En ciertas modalidades, las enzimas son fitasas. También se proporcionan métodos para utilizar estas composiciones para liberar fosfatos de la materia orgánica que contiene ácido fítico.

Phytase mutants

Provided are mutants PHY1, PHY4 and PHY5 of a wild-type phytase APPA. After being treated for 10min at 80°C , the residual enzyme activities of the mutants PHY1, PHY4 and PHY5 are respectively higher by 33.85%, 53.11% and 75.86% compared with that of APPA-M; after being treated for 5min at 85°C, the residual enzyme activities of the mutants PHY1, PHY4 and PHY5 are respectively higher by 14.89%, 28.45% and 44.94% compared with that of APPA-M, and the heat resistance of these mutants is significantly higher than that of APPA-M.

Method for conversion of phytate to inorganic phosphate, method for preparing fodder for animal and method for preparing food for humans

FIELD: nutrition biochemistry, food technology. SUBSTANCE: invention relates to methods for conversion of phytate to inorganic phosphate and can be used in methods for preparing fodder for animals and food for humans. Method involves preparing a phytase-containing suspension, phytate-containing foodstuff and liquid. Suspension is prepared with pH 2.0-8.0 wherein 60-1000 weight part of liquid is added per 100 weight parts of phytate-containing foodstuff. A mixture containing water and water non-mixing organic solvent with boiling point 20-100 o C is used as a liquid. The content of water non-mixing organic solvent in phytate-containing mixture is 20-85 weight parts per each 100 weight parts of indicated mixture. Then suspension is stirred and product is dried. Method provides the development of commercially acceptable method used for conversion of phytate to inorganic phosphate due to reducing energy consumption. EFFECT: improved conversion method. 16 cl, 1 ex ЭтЕЭ9$сСс ПЧ сэ (19) РОССИЙСКОЕ АГЕНТСТВО ПО ПАТЕНТАМ И ТОВАРНЫМ ЗНАКАМ ВИ "” 2 236 146. (51) МПК? 13) С2 А 23 4 1/12, 1/14, А 23 К 1/165 12) ОПИСАНИЕ ИЗОБРЕТЕНИЯ К ПАТЕНТУ РОССИЙСКОЙ ФЕДЕРАЦИИ (21), (22) Заявка: 20011071383, 17.08.1999 (24) Дата начала действия патента: 17.08.1999 (30) Приоритет: 19.08.1998 СВ 9818126.6 (43) Дата публикации заявки: 21.03.2003 (46) Дата публикации: 20.09.2004 (56) Ссылки: ЕР 0380343 А, 01.08.1990. 1$ 3733207 А, 15.05.1973. СВ 2180241 А, 25.03.1987. 1$ 3297548 А, 10.01.1967. Классификация и номенклатура ферментов. Отчет Комиссии по ферментам Международного биохимического союза 1961. - М.: Издательство иностранной литературы, 1962, с. 128. (85) Дата перевода заявки РСТ на национальную фазу: 19.03.2001 (86) Заявка РСТ: 1В 99/01679 (17.08.1999) (87) Публикация РСТ: М/О 00/10404 (02.03.2000) (98) Адрес для переписки: 129010, Москва, ул. Большая Спасская, 25, стр.3, ООО "Юридическая фирма Городисский и Партнеры", Е.В.Томской (72) Изобретатель: МАЭНЗ Дэвид Дэниел (СА), КЛАССЕН Генри ...

Production of enzymes in seeds and their use

A method of catalyzing in vitro reactions using seeds containing enhanced amounts of enzymes is disclosed. The method involves adding transgenic, non-wild type seeds, preferably in a ground form, to a reaction mixture and allowing the enzymes in the seeds to increase the rate of reaction. By directly adding the seeds to the reaction mixture the method provides a solution to the expensive and problematic process of extracting and purifying the enzyme. Methods of treatment are also provided whereby a subject lacking a sufficient supply of an enzyme is administered the enzyme in the form of seeds containing enhanced amounts of the enzyme.

MAIZE INFUSION PROCESS AND APPARATUS.

Pre-treatment metalloprotease preparation pack

Recombinant bacterial phytase and use thereof

Method for explication of phytaze in plants

Phytase formulation

A liquid enzyme formulation, an enzyme granule formulation, methods for manufacturing enzyme granules using a fluid bed dryer, wherein the enzyme granules are thermostable without the need for a thermostable coating is provided. The enzyme granules are phytase granules used in the manufacturing of an animal feed, wherein the phytase granule is thermostable without the need for a thermostable coating and the phytase retains about 63% to about 134% of its activity after pelleting at 80°C.

Phytases, nucleic acids encoding them and methods for making and using them

本发明的发明名称是肌醇六磷酸酶、编码它们的核酸及制备和使用它们的方法。本发明涉及肌醇六磷酸酶、编码它们的多核苷酸、本发明的多核苷酸和多肽的应用、以及这类多核苷酸和多肽的产生和分离。特别是，本发明提供了在高温条件下具有肌醇六磷酸酶活性的多肽，和暴露于高温后保持活性的肌醇六磷酸酶。本发明的肌醇六磷酸酶除了在更高的温度下，在低温下也可以是耐热的和/或热稳定的。本发明的肌醇六磷酸酶可被用在食品中以改善富肌醇六磷酸成分的饲养价值。本发明的肌醇六磷酸酶可被配制为例如帮助肌醇六磷酸消化的食物或饲料或补充剂。本发明的食物和饲料可以是粒料、液体、粉末等形式。在一个方面，本发明的肌醇六磷酸酶在粒化期间对热变性稳定；并且这减少肌醇六磷酸酶产品的成本，同时保持体内功效和在饲料中检测到活性。

Phytases, nucleic acids encoding them and methods for making and using them

The invention provides isolated and recombinant phytase enzymes. In one aspect, the phytases are produced by modification of the wild type appA of E. coli. The enzyme can be produced from recombinant host cells. The phytases of the invention can be used to aid in the digestion of phytate where desired. In particular, the phytases of the invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients. The phytases of the invention can be thermotolerant and/or thermostable. Also provided are methods for obtaining a variant polynucleotide encoding a phytase and for obtaining a phytase with thermostability or thermotolerant at high or low temperatures.

Animal feed compositions and uses thereof.

La presente invención se refiere a composiciones de pienso para animales que comprenden polipéptidos que tienen actividad lisozima y polipéptidos que tienen actividad fitasa y usos de las mismas.

FOOD-CONTAINING ENZYMES

1. Содержащий ферменты гранулят для корма, частицы которого включают ! A) содержащее ферменты ядро с содержанием воды ниже 15 мас.% в расчете на массу ядра, содержащего ферменты, которое содержит ! i) от 50 до 96,9 мас.%, по меньшей мере, одного твердого носителя, подходящего для корма, ! ii) от 0,1 до 20 мас.%, по меньшей мере, одного нейтрального производного целлюлозы, растворимого в воде, ! iii) от 3 до 49,9 мас.%, по меньшей мере, одного фермента, причем массовые части i), ii) и iii) являются соответственно в расчете на не водные составляющие ядра; и ! B) по меньшей мере, одно гидрофобное покрытие, расположенное на поверхности ядра, которое включает, по меньшей мере, один гидрофобный материал, выбираемый из восков, насыщенных жирных кислот, сложных эфиров насыщенных жирных кислот, полиолефинов и полиамидов в количестве, по меньшей мере, 70 мас.% в расчете на общую массу покрытия. ! 2. Содержащий ферменты гранулят по п.1, причем массовое соотношение ядра к покрытию находится в области от 70:30 до 99:1. ! 3. Содержащий ферменты гранулят по п.1 или 2, причем гидрофобное покрытие до, по меньшей мере, 70% состоит из одного или нескольких триглицеридов насыщенных кислот. ! 4. Содержащий ферменты гранулят по п.1 или 2, в котором производным целлюлозы, растворимым в воде, является метилцеллюлоза. ! 5. Содержащий ферменты гранулят по п.1 или 2, в котором частицы имеют средний размер частиц в области от 100 до 2000 мкм. ! 6. Содержащий ферменты гранулят по п.1 или 2, причем носитель включает, по меньшей мере, один полимерный углевод, нерастворимый в воде. ! 7. Содержащий ферменты гранулят по п.1 или 2, причем ферментом является фосфатаза [Е.С. 3.1.3], в частности фитаза. ! 8. Содержащий ферменты РОССИЙСКАЯ ФЕДЕРАЦИЯ (19) RU (11) 2008 113 840 (13) A (51) МПК A23K A23K 1/165 (2006.01) 1/00 (2006.01) ФЕДЕРАЛЬНАЯ СЛУЖБА ПО ИНТЕЛЛЕКТУАЛЬНОЙ СОБСТВЕННОСТИ, ПАТЕНТАМ И ТОВАРНЫМ ЗНАКАМ (12) ЗАЯВКА НА ИЗОБРЕТЕНИЕ (21), (22) Заявка: 2008113840/13, 11.09.2006 (30) Конвенционный ...

A process for steeping cereals with a new enzyme preparation

Glycosylation as a stabilizer for phytase

The present teachings provide modified enzymes, preferably phytases, which have increased stability, hypothesized to arise from increased glycosylation. The enzymes can be modified to introduce or increase the number of glycosylation sites in the amino acid sequence, or glycosylation can be increased by the use of specific host production methods, or both. The enzymes of the present teachings have an increased stability after treatment at elevated temperature, which can be measured by inactivity reversibility or percent recovery following a treatment such as heating. The enzymes of the present teachings find application for example in food, feed, and feed pelleting.

Granulates containing enzymes for animal food

本发明涉及适合作为动物饲料的新的含酶细粒及其生产方法。本发明还涉及含酶细粒在动物饲料组合物中的用途，以及尤其是可使用含酶细粒得到的造粒的动物饲料组合物。酶细粒包含：A)水含量低于15重量％，优选为1－12重量％，尤其是3－10重量％，特别是5－9重量％的含酶核，其中所述量基于包含如下物质的含酶核的重量：i)50－96.9重量％，优选55－94.85重量％，尤其是60－89.7重量％的至少一种适用于动物饲料的固体载体材料，ii)0.1－10重量％，优选0.15－5重量％，尤其是0.2－2重量％，特别是0.3－1重量％的至少一种水溶性的天然纤维素衍生物，iii)3－49.9重量％，经常为5－49.85重量％，尤其是10－44.8重量％，特别是10－39.7重量％的至少一种酶，在每种情况下i)、ii)和iii)的重量百分数基于该核的非水性组分；和B)至少一个配置在该核表面上的疏水涂层，该涂层包含至少一种选自蜡、饱和脂肪酸、饱和脂肪酸的酯、聚烯烃和聚酰胺的疏水材料，且其量基于该涂层的总重量为至少70重量％，优选至少80重量％，尤其是至少90重量％，特别是至少99重量％。

Novel phytase

The invention provides a purified phytate enzyme derived from Escherichia coli B. The enzyme has a molecular weight of about 47.1 kilodaltons and has phytase activity (SEQ ID NO:2). The enzyme can be produced from native or recombinant host cells and can be used to aid in the digestion of phytate where desired. In particular, the phytase of the present invention can be used in animal feed.

Phytase from germinated soybeans

The invention relates to a class of phytate-degrading enzymes which are endogenously present in soya flour, soybeans, germinated soybeans or fractions thereof, to a method for obtaining such enzymes as well as to the use of these enzymes in feed and food applications.

Phytases, nucleic acids encoding them and methods for making and using them

This invention relates to phytases, polynucleotides encoding them, uses of the polynucleotides and polypeptides of the invention, as well as the production and isolation of such polynucleotides and polypeptides. In particular, the invention provides polypeptides having phytase activity under high temperature conditions, and phytases that retain activity after exposure to high temperatures. The phytases of the invention can be thermotolerant and/or thermostable at low temperatures, in addition to higher temperatures. The phytases of the invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients. The phytases of the invention can be formulated as foods or feeds or supplements for either to, e.g., aid in the digestion of phytate. The foods or feeds of the invention can be in the form of pellets, liquids, powders and the like. In one aspect, phytases of the invention are stabile against thermal denaturation during pelleting; and this decreases the cost of the phytase product while maintaining in vivo efficacy and detection of activity in feed.

Novel liquefaction process for producing alcohol from colloidal corn

本发明公开了一种胶质玉米生产酒精的辅助液化酶，其由质量份的原料组成：果胶酯酶25～35份，果胶裂解酶25～35份，木聚糖酶10～20份，植酸酶5～15份，纤维素酶8～15份，缓冲体系3～10份。本申请的辅助液化酶，明显降低了液化醪的粘度，明显缩短了液化时间，酒精生产中蒸煮液化工段蒸汽耗用量占吨酒精全部蒸汽耗用量的70％左右，由于缩短液化时间，可以大大减少耗能，发酵终了残淀粉残糊精分别比添加前降低了60％～50％左右，且发酵终了酒份比添加之前提高了0.3‑0.4％(v/v)，DDS工段固形物蒸发浓缩粘度降低明显，缩短浓缩干燥时间，节约蒸汽用量，厌氧发酵池甲烷菌代谢正常。本申请的辅助液化酶具有突出的技术效果。