17-01-2019 дата публикации
Номер: US20190015835A1
Принадлежит:
The present invention provides a portable thermal cycling device for quickly changing and regulating temperature, which is used for deoxyribonucleic acid (DNA) detection and amplification. The device adopts the concept of an electrical impedance method for detection, utilizes the spinning coating and electrospinning nanowires technologies to directly fabricate a thin film type thermal cycling device, and under the cooperation of a laser direct writing technology for patterning definition, enables the device to have the function of quickly raising/reducing temperature at one time for DNA amplification. Moreover, the present invention is provided with a micro-fluid channel and an electrical control module to adapt the reaction states of different biological DNA 1. A portable thermal cycling device for quickly changing and regulating temperature , comprising:A substrate, made from a transparent material with a high optical penetration rate, and coated with graphene;A temperature control layer, disposed on one side of the substrate, and provided with a laser defined electrical impedance area, wherein the electrical impedance area comprises a first resistor area, a second resistor area, and a third resistor area;A sensing layer, disposed on one side of the temperature control layer, and provided with a nanowires defined nano-sensing area, wherein the nano-sensing area comprises a first sensing area, a second sensing area, and a third sensing area; andA microchannel layer, disposed on one side of the sensing layer, and provided with a picosecond laser defined microchannel area, wherein the microchannel area comprises a first microchannel, a second microchannel, and a third microchannel; andThe electrical impedance area, the nano-sensing area and the microchannel area have the same range, and are sequentially sealed together.2. The portable thermal cycling device for quickly changing and regulating temperature as claimed in claim 1 , wherein the transparent material with a ...
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