01-05-2014 дата публикации
Номер: US20140119633A1
Automated islet measurement systems (AIMS) in combination with tissue volume analysis (TVA) software effectively gauges volumetric and size-based data to generate heretofore unavailable information regarding, for example, populations of islet cells, stem cells and related desiderata. 1. An automatic tissue scanning camera (ATSC) system , which comprises , in combination:a monochrome CCD camera;a horizontally-mounted culture flask;trans-illumination means, which means exceeds the deep field image size by a factor of at least two;wherein placement of the flask between the illuminator and camera coaxially and opposed on crossed, ultra precision mechanical stages driven by processing means, generates rectangular arrays of continuous digital images, and;whereby the density of any one cell, for example an islet cell, is derived as a function of scattering, absorption, and thickness.2. A system according to claim 1 , further comprising claim 1 , tissue volume analysis (TVA) software claim 1 , using thresholding to identify individual cells claim 1 , for example islet cells claim 1 , and to evaluate cross-sectional linear size of cells claim 1 , for example islet cells claim 1 , via an algorithmic simulacrum of manual counting under microscopy.3. A system according to claim 2 , wherein said TVA software measures the optical transmission of each pixel within each cell claim 2 , for example islet cell claim 2 , using this to calculate thickness of tissue at that pixel based upon optical extinction; and claim 2 ,based on constant pixel area, calculates volume represented by respective pixels;whereby voxels, volume elements, are then summed to generate resultory total cell, for example islet cell, volume.4. A system according to claim 1 , wherein an automated image measurement algorithm is employed.5. A system according to claim 2 , wherein an automated image measurement algorithm is employed.6. A system according to claim 3 , wherein an automated image measurement algorithm is ...
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