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Небесная энциклопедия

Космические корабли и станции, автоматические КА и методы их проектирования, бортовые комплексы управления, системы и средства жизнеобеспечения, особенности технологии производства ракетно-космических систем

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Мониторинг СМИ

Мониторинг СМИ и социальных сетей. Сканирование интернета, новостных сайтов, специализированных контентных площадок на базе мессенджеров. Гибкие настройки фильтров и первоначальных источников.

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05-01-2012 дата публикации

Portable sample analyzer cartridge

Номер: US20120003730A1

Автор: Aravind Padmanabhan, Cleopatra Cabuz, James A. Cox

Принадлежит: Honeywell International Inc

A sample analyzer cartridge for use at a point of care of a patient such as in a doctor's office, in the home, or elsewhere in the field. By providing a removable and/or disposable cartridge with all of the needed reagents and/or fluids, the sample analyzer can be reliably used outside of the laboratory environment, with little or no specialized training.

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Номер записи: 1

02-02-2012 дата публикации

Fluorescence detection device and fluorescence detection method

Номер: US20120025098A1

Автор: Kyouji Doi, Shigeyuki Nakada

Принадлежит: Mitsui Engineering and Shipbuilding Co Ltd

When receiving fluorescence emitted by a measurement object irradiated with laser light emitted from a laser light source unit, a fluorescence detection device generates a modulation signal for modulating the intensity of the laser light and modulates the laser light using the modulation signal. The fluorescence detection device obtains a fluorescent signal of the fluorescence emitted by the measurement object irradiated with the laser light, and calculates, from the fluorescent signal, a fluorescence intensity and the phase delay of the fluorescence with respect to the modulation signal. At the time, the fluorescence detection device controls the operation amounts of the signal level of a DC component of the modulation signal and the gain of amplification just after the output of the fluorescent signal so that the value of a fluorescence intensity signal falls within a preset range. After the operation amounts are settled, the fluorescence detection device calculates the fluorescence intensity and then calculates the fluorescence relaxation time of the fluorescence emitted by the measurement object using the phase delay.

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Номер записи: 2

09-02-2012 дата публикации

Method and apparatus for multi-parameter data analysis

Номер: US20120035859A1

Автор: Nicholas Thomas

Принадлежит: GE Healthcare UK Ltd

In one aspect, the present invention relates to a method 200 for identifying one or more phenotypes from a multi-parameter data set. The method 200 comprises measuring 202 correlation between pairs of parameters within the multi-parameter data set, modifying 204 correlated parameter values within a predetermined multi-parameter data analysis set to form an analysis parameter set, and analysing 206 the multi-parameter data set using the analysis parameter set to identify one or more phenotypes from the multi-parameter data set. Various embodiments of the present invention may, for example, be used in an automated high-content screening (HCS) apparatus 100 for biological cellular analysis.

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Номер записи: 3

15-03-2012 дата публикации

Single-cell microchamber array

Номер: US20120065082A1

Автор: Albert P. Pisano, Franciscus Albertus Kuypers, Won Chul Lee

Принадлежит: Childrens Hospital Oakland Research Center

An apparatus for analyzing individual cell composition in a heterogeneous cell population may include, in one embodiment, a deposition plate having an array of microwells disposed therein, and a cover plate substantially overlying the deposition plate. A pair of electrodes may be associated with one or more of the microwells, and may be configured to generate an electric field within the associated microwell.

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Номер записи: 4

29-03-2012 дата публикации

Apparatus for high-throughput suspension measurements

Номер: US20120073972A1

Автор: Fraser Mcneil Watson

Принадлежит: Malvern Instruments Ltd

A high-throughput optical suspension characterization instrument is disclosed, which can include hydraulically separate and at least partially transparent sample containers. A selection mechanism is operative to selectively direct light from a light source ( 12 ) through different ones of the sample containers along an optical axis, and an off-axis scattering detector ( 38,24 ) is responsive to scattered light from the light source after it has interacted with a sample. Phase analysis light scattering is used to determine the electrophoretic mobility and zeta potential of samples. A second instrument is disclosed, wherein all sample containers are illuminated simultaneously. Transmitted light is collected by a camera. The electrophoretic mobility and hydrodynamic size of the samples may be determined.

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Номер записи: 5

10-05-2012 дата публикации

High fidelity colour imaging of microbial colonies

Номер: US20120114218A1

Автор: Alasdair Graham Hayden-Wright, Philip Atkin

Принадлежит: SYNOPTICS Ltd

A system and an associated method of imaging microbial colonies in high fidelity and in colour. Differently coloured light sources are turned on and off in sequence and for each illumination colour an image is captured by a monochrome camera. The resultant respective monochrome images are combined into a composite colour image of the microbial colonies.

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Номер записи: 6

24-05-2012 дата публикации

Magnetic Sector Mass Spectrometry Based Multi-Parametric Particle Analyzer

Номер: US20120126114A1

Автор: Dmitry R. Bandura, Scott D. Tanner, Vladimir I. Baranov

Принадлежит: PerkinElmer Health Sciences Inc

An analytical instrument has a sample introduction system for generating a stream of particles from a sample and an ionization system for receiving the particles. The ionization system is operable to atomize the particles received from the sample introduction system and ionize atoms from the atomized particles. The instrument has an ion pretreatment system and a magnetic sector mass analyzer comprising an array detector. The ion pretreatment system is adapted to transport ions generated by the ionization system to the mass analyzer. The mass analyzer is adapted to detect a transient signal of at least one element from individual particles from said stream by performing mass analysis on the ions from the atomized particles. The magnetic sector mass analyzer is adapted determine an amount of said at least one element from an individual particle using the transient signal detected during mass analysis of the ions from said individual particle.

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Номер записи: 7

31-05-2012 дата публикации

Particle counting method

Номер: US20120133936A1

Автор: Takehiro Imai, Tomonobu Matsuda, Toshiyuki Abe, Tsutomu Nakajima

Принадлежит: Rion Co Ltd

A particle counting method that can count the number of the particles precisely. The method discriminates a wave pattern of the scattered light from a normal particle (subject of the counting) and a wave pattern of the light scattered by the agitation such as a floating particle, a radiation or changes in the intensity of the light. In one embodiment, a method for counting particles is disclosed which irradiates a light to a sample gas, detects a scattered light from a particle included in the sample gas by a photoelectric conversion device, counts the number of the particles of every particle size division by the output voltage wave pattern of the photoelectric conversion device, calculate a time difference (Ta−T 1 ) from a point (T 1 ) being a peak of output voltage wave pattern and a point (Ta) being a falling detection threshold (A), when the time difference (Ta−T 1 ) is beyond counting cancellation time (B), the output voltage wave pattern is not counted as a particle.

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Номер записи: 8

14-06-2012 дата публикации

Blood Measuring Apparatus

Номер: US20120145536A1

Автор: Yoshihiro Niiyama

Принадлежит: Nihon Kohden Corp

A blood measuring apparatus includes: first and second chambers which communicate with each other through an aperture; first and second electrodes which are disposed respectively in the first chamber and the second chamber; and a controller: which performs blood measurement by causing a current to flow between the first and second electrodes in a state where diluted blood is contained in the first chamber and diluting solution is contained in the second chamber; and which performs electrolysis by applying a voltage between the first and second electrodes in a state where diluting solution is contained in the first and second chambers, thereby producing washing solution, and which performs washing on at least the aperture, and the first and second chambers by using the produced washing solution.

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Номер записи: 9

19-07-2012 дата публикации

Multiple laminar flow-based particle and cellular separation with laser steering

Номер: US20120183947A1

Автор: Amy Anderson, Daniel Mueth, Jessica Shireman, Joseph Plewa, Lewis Gruber, Neil Harris Rosenbaum

Принадлежит: Arryx Inc

The invention provides a method, apparatus and system for separating cellular components, and can be combined with holographic optical trapping manipulation or other forms of optical tweezing. One exemplary method includes providing a first flow having a plurality of components; providing a second flow; contacting the first flow with the second flow to provide a first separation region; and differentially sedimenting a first cellular component of the plurality of components into the second flow while concurrently maintaining a second cellular component of the plurality of components in the first flow. The second flow having the first cellular component is then differentially removed from the first flow having the second cellular component. Holographic optical traps may also be utilized in conjunction with the various flows to move selected components from one flow to another, as part of or in addition to a separation stage.

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Номер записи: 10

02-08-2012 дата публикации

Three-dimensional (3d) hydrodynamic focusing using a microfluidic device

Номер: US20120196314A1

Автор: Ahmad Ahsan Nawaz, Tony Jun Huang, Xiaole Mao

Принадлежит: PENN STATE RESEARCH FOUNDATION

A microfluidic device comprises inlets for a sample flow and an out-of-plane focusing sheath flow, and a curved channel section configured to receive the sample flow and out-of-plane focusing sheath and to provide hydrodynamic focusing of the sample flow in an out-of-plane direction, the out-of-plane direction being normal to a plane including the curved channel. Examples of the invention also include improved flow cytometers.

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Номер записи: 11

09-08-2012 дата публикации

Minimally invasive cytometry system with qcl inspection of single cells for cancer detection

Номер: US20120202277A1

Автор: John Heanue, Matthias Wagner

Принадлежит: 1087 SYSTEMS Inc

This disclosure concerns a minimally invasive cytometry system including a handling system that presents single cells to at least one QCL laser source. The QCL laser source is configured to deliver light to a cell within the cells in order to induce vibrational bond absorption in one or more analytes within the cell. The cytometry system also includes a detection facility that detects the mid-infrared wavelength light transmitted by the cell and identifies the cell as either cancerous or non-cancerous.

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Номер записи: 12

06-09-2012 дата публикации

Device for Determining Particle Sizes

Номер: US20120224166A1

Автор: Martin Heine, Stefan Manz

Принадлежит: BUEHLER AG

Method for measuring particle size distributions, in particular for the optical measurement of wide particle size distributions of bulk materials such as cereals, cereal milling products, cereal products and the like, which is intended to enable the measurement of particle size distributions which vary by orders of magnitude. To address this problem, a sample of isolated particles is optically detected in an arrangement by means of at least two measurement methods, wherein preferably detection of the contours of the particles and laser diffraction take place at the same time.

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Номер записи: 13

01-11-2012 дата публикации

Multi-Way Sorter System and Method

Номер: US20120276621A1

Автор: Gerrit Jan Van Den Engh

Принадлежит: Becton Dickinson and Co

Provided herein are improved multi-way cell sorter systems and methods. For example, provided are systems and methods for the collection of cells that are sorted in multiple directions. The systems and methods allow for the construction of a multi-way sorter (e.g., a ten-way sorter in the space that currently only allows four-way sorting). In addition the device may actively sense the arrival of drops (with cells of interest) at a sample tube, and trigger an alarm when the drops' arrivals deviate from an expected pattern.

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Номер записи: 14

08-11-2012 дата публикации

White Blood Cell Analysis System and Method

Номер: US20120282598A1

Автор: Giacomo Vacca, Jiong Wu

Принадлежит: ABBOTT LABORATORIES

Systems and methods for analyzing blood samples, and more specifically for performing a white blood cell (WBC) differential analysis. The systems and methods screen WBCs by means of fluorescence staining and a fluorescence triggering strategy. As such, interference from unlysed red blood cells (RBCs) and fragments of lysed RBCs is substantially eliminated. The systems and methods also enable development of relatively milder WBC reagent(s), suitable for assays of samples containing fragile WBCs. In one embodiment, the systems and methods include: (a) staining a blood sample with an exclusive cell membrane permeable fluorescent dye, which corresponds in emission spectrum to an excitation source of a hematology instrument; (b) using a fluorescence trigger to screen the blood sample for WB Cs; and (c) using measurements of (1) axial light loss, (2) intermediate angle scatter, (3) 90° polarized side scatter, (4) 90° depolarized side scatter, and (5) fluorescence emission to perform a differentiation analysis.

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Номер записи: 15

06-12-2012 дата публикации

Particle Analysis in an Acoustic Cytometer

Номер: US20120304749A1

Автор: Gregory Kaduchak, Michael D. Ward

Принадлежит: Los Alamos National Security LLC

The present invention is a method and apparatus for acoustically manipulating one or more particles.

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Номер записи: 16

06-12-2012 дата публикации

Multiple Flow Channel Particle Analysis System

Номер: US20120307244A1

Автор: Blair Morad, Donald Francis Perrault, JR., Emanuel Tito Mendes Machado, Johnathan Charles Sharpe, Rudolf Hulspas

Принадлежит: Cytonome ST LLC

A microfluidic multiple channel particle analysis system ( 1 ) which allows particles ( 2 ) from a plurality of particle sources ( 3 ) to be independently simultaneously entrained in a corresponding plurality of fluid streams ( 4 ) for analysis and sorting into particle subpopulations ( 5 ) based upon one or more particle characteristics ( 6 ).

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Номер записи: 17

20-12-2012 дата публикации

Analysis device and reagent container

Номер: US20120321513A1

Автор: Nobuhiro Kitagawa, Takaaki Nagai, Yuichi Hamada

Принадлежит: Sysmex Corp

An analysis device for particle analysis, configured in such a manner that a reagent container is set in the device by inserting the reagent container from a side surface of the device toward the inside thereof, the reagent container having, near the forward end thereof, a suction pipe entrance portion into which a suction pipe can enter. The analysis device is provided with: a reagent container holding portion which holds the reagent container inserted from the suction pipe entrance portion side; and the suction pipe which enters, from above, the suction pipe entrance portion of the reagent container held by the reagent container holding portion and which sucks a reagent within the reagent container. The reagent container holding portion includes a guide member for guiding the insertion of the reagent container, which is inserted from the suction pipe entrance portion side, into the reagent container holding portion.

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Номер записи: 18

03-01-2013 дата публикации

Systems and methods for sample display and review

Номер: US20130002847A1

Автор: Adam Yie, David Zahniser, Michael Zahniser

Принадлежит: Constitution Medical Inc

Methods and systems for displaying images of cells in a sample include obtaining a plurality of images of cells in the sample, where each image corresponds to one of the cells in the sample, determining values of at least one property for each of the cells based on the plurality of images, arranging the plurality of images to form a first image array, where the images are ordered in the first image array based on the values of the at least one property, displaying the first image array, sorting the plurality of images to form a second image array in which an ordering of the images is different from the first image array, and displaying the second image array, where the sample includes blood and the cells include red blood cells.

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Номер записи: 19

14-03-2013 дата публикации

Fine particle measuring apparatus

Номер: US20130065269A1

Автор: Nao Nitta

Принадлежит: Sony Corp

A fine particle measuring apparatus is provided. The fine particle measuring apparatus includes a detection unit configured to detect light emitted from a fine particle and a processing unit having a memory device storing instructions which when executed by the processing unit, cause the processing unit to calculate a corrected intensity value of the detected light and generate spectrum data based on the corrected intensity value.

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Номер записи: 20

21-03-2013 дата публикации

Method and apparatus for measuring optical properties of particles of a dispersion

Номер: US20130070243A1

Автор: Wolfgang Goehde

Принадлежит: Partec GmbH

Disclosed is apparatus for measuring optical properties of particles of a flowable dispersion using a measuring cuvette. The dispersion flows through the central inner chamber of the cuvette. Two laser light beams, which are offset 90 degrees to one another, illuminate the inner chamber of the cuvette, so as to illuminate a particle, regardless of its orientation, in a way that balances out form factor errors.

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Номер записи: 21

02-05-2013 дата публикации

Biochip

Номер: US20130104632A1

Автор: Chin-Kuan Lin, Hsiang-Chia Chen, Pai-Yang Lin, Sheng-Hsiung Weng, Yih-Far Chen

Принадлежит: Wistron Corp

A biochip including a chip body, a first electrode and a second electrode is provided. The body has a first accommodating cavity, a second accommodating cavity and a micro-fluid channel. The micro-fluid channel is connected with the first accommodating cavity and the second accommodating cavity. The first electrode has a first end and a second end. The first end is used for contacting a first probe of a detection apparatus. The second end is positioned in the first accommodating cavity. The second electrode has a third end and a forth end. The third end is used for contacting a second probe of the detection apparatus. The forth end is positioned in the second accommodating cavity.

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Номер записи: 22

13-06-2013 дата публикации

Methods for sorting particles

Номер: US20130149736A1

Автор: Gary Durack, Gary P. Vandre, Jeffrey D. Wallace, Jeremy T. Hatcher, Lon A. Westfall, Niraj Nayak

Принадлежит: INGURAN LLC

A multi-channel apparatus for classifying particles according to one or more particle characteristics. The apparatus comprises a plurality of flow cytometry units, each of which is operable to classify particles in a mixture of particles by interrogating a stream of fluid containing the particles with a beam of electromagnetic radiation. The flow cytometry units share an integrated platform comprising at least one of the following: (1) a common supply of particles; (2) a common housing; (3) a common processor for controlling operation of the units; (4) a common processor for receiving and processing information from the units; and (5) a common fluid delivery system. The integrated platform can include a common source of electromagnetic radiation. A method of the invention comprises using a plurality of flow cytometry units sharing the integrated platform to perform a flow kilometric operation, such as analyzing or sorting particles.

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Номер записи: 23

27-06-2013 дата публикации

Imaging and evaluating embryos, oocytes, and stem cells

Номер: US20130162795A1

Автор: Barry Behr, Connie C. Wong, Kevin E. Loewke, Renee A. Reijo-Pera, Thomas M. Baer

Принадлежит: Leland Stanford Junior University

Methods, compositions and kits for determining the developmental potential of one or more embryos or pluripotent cells and/or the presence of chromosomal abnormalities in one or more embryos or pluripotent cells are provided. These methods, compositions and kits find use in identifying embryos and oocytes in vitro that are most useful in treating infertility in humans.

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Номер записи: 24

27-06-2013 дата публикации

Particle monitoring with secure data logging

Номер: US20130166251A1

Автор: Eric Robert Wright, Paul H. Yates, Robert A. Latimer, Walter D. MODIC

Принадлежит: Hach Co

A data logging monitoring instrument and related methods are described. One aspect provides a particle counting instrument, including: one or more sensors; a storage system; and a processing system coupled to the storage system, the processing system being configured to accumulate measurement data ascertained via the one or more sensors in the storage system into one or more log files; wherein the one or more log files are stored in a secure file format that is compatible with other non-particle counting instrument information processing device applications. Other embodiments are described.

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Номер записи: 25

04-07-2013 дата публикации

Convex Lens-Induced Confinement for Measuring Distributions of Molecular Size

Номер: US20130170026A1

Автор: Adam E. Cohen, Sabrina R. Leslie

Принадлежит: Individual

A curved surface is placed tangent to a slide and displaces a sample liquid from the point or line of contact outward. Imaging indicates a region where fluorescence is observed, and the location of the fluorescence indicates the molecular size. The radius of curvature of the lens is known, the distance from the (center) point of contact of the observed fluorescence is measured with a microscope and the distance of the lens surface to the slide's surface can then be calculated. This distance represents the size of the molecule or ensemble of molecules emitting. Similarly, absorbance, etc. could be measured with a light source below the slide.

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Номер записи: 26

18-07-2013 дата публикации

Liquid crystals with switchable wettability for cell sorting

Номер: US20130183711A1

Автор: Angele Sjong

Принадлежит: EMPIRE TECHNOLOGY DEVELOPMENT LLC

Disclosed are methods and apparatuses for identifying and sorting cells based on the cells' response to an external stimulus. Cellular adherence to liquid crystals with tunable wettability is measured before and after an induced change in the liquid crystal wettability. The cell-based liquid crystal reorientation can be measured and used for monitoring and sorting of cells in a label-free manner, and thus provides a positive method for selecting cells, such as stem cells, for use in tissue engineering applications.

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Номер записи: 27

18-07-2013 дата публикации

Cell processing apparatus, sample preparation apparatus, and cell analyzer

Номер: US20130183747A1

Автор: Hironori Kobayashi, Junyi Ding, Koki Tajima, Masakazu Fukuda, Ryuichiro Ebi

Принадлежит: Sysmex Corp

A cell processing apparatus includes a storage container that contains liquid L including a biological sample; a filter that prevents a first cell C 1 in the biological sample from passing and allows a second cell C 2 having a smaller diameter than that of the first cell C 1 to pass; and a filtration cylinder for separating, in the storage container and via the filter, the liquid L into a first liquid L 1 mainly including the first cell C 1 and a second liquid L 2 mainly including the second cell C 2 . A measurement target cell filtered by the filter among other cells can be easily collected.

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Номер записи: 28

15-08-2013 дата публикации

Flow Cytometry For High Throughput Screening

Номер: US20130210672A1

Автор: Bruce S. Edwards, Frederick W. Kuckuck, Larry A. Sklar

Принадлежит: STC UNM

The present invention, provides a flow cytometry apparatus for the detection of particles from a plurality of samples comprising: means for moving a plurality of samples comprising particles from a plurality of respective source wells into a fluid flow stream; means for introducing a separation gas between each of the plurality of samples in the fluid flow stream; and means for selectively analyzing each of the plurality of samples for the particles. The present invention also provides a flow cytometry method employing such an apparatus.

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Номер записи: 29

22-08-2013 дата публикации

Acoustic waves in microfluidics

Номер: US20130213488A1

Автор: Achim Wixforth, Adam R. Abate, David A. Weitz, Jeremy Agresti, Lothar Schmid, Thomas Franke

Принадлежит: Harvard College

Various aspects of the present invention relate to the control and manipulation of fluidic species, for example, in microfluidic systems. In one set of embodiments, droplets may be sorted using surface acoustic waves. The droplets may contain cells or other species. In some cases, the surface acoustic waves may be created using a surface acoustic wave generator such as an interdigitated transducer, and/or a material such as a piezoelectric substrate. The piezoelectric substrate may be isolated from the microfluidic substrate except at or proximate the location where the droplets are sorted, e.g., into first or second microfluidic channels. At such locations, the microfluidic substrate may be coupled to the piezoelectric substrate (or other material) by one or more coupling regions. In some cases, relatively high sorting rates may be achieved, e.g., at rates of at least about 1,000 Hz, at least about 10,000 Hz, or at least about 100,000 Hz, and in some embodiments, with high cell viability after sorting.

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Номер записи: 30

22-08-2013 дата публикации

Spatially Correlated Light Collection from Multiple Sample Streams Excited with a Line Focused Light Source

Номер: US20130214176A1

Автор: Andrew P. Shreve, Gabriel P. Lopez, Pearlson Prashanth Austin Suthanthiraraj, Steven Wayde GRAVES

Принадлежит: STC UNM

An affordable flow cytometry system with a significantly increased analytical rate, volumetric sample delivery and usable particle size including a light beam that interrogates multiple flow streams so as to provide excitation across all of the streams, and an optical objective configured to collect light from the sample streams and image the light onto an array detector.

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Номер записи: 31

22-08-2013 дата публикации

Waveguide-based detection system with scanning light source

Номер: US20130215425A9

Автор: Reuven Duer

Принадлежит: PLC Diagnostics Inc

The invention provides methods and devices for generating optical pulses in one or more waveguides using a spatially scanning light source. A detection system, methods of use thereof and kits for detecting a biologically active analyte molecule are also provided. The system includes a scanning light source, a substrate comprising a plurality of waveguides and a plurality of optical sensing sites in optical communication with one or more waveguide of the substrate, a detector that is coupled to and in optical communication with the substrate, and means for spatially translating a light beam emitted from said scanning light source such that the light beam is coupled to and in optical communication with the waveguides of the substrate at some point along its scanning path. The use of a scanning light sources allows the coupling of light into the waveguides of the substrate in a simple and cost-effective manner.

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Номер записи: 32

29-08-2013 дата публикации

Sperm processing methods

Номер: US20130224734A1

Автор: Gary Durack, Gary P. Vandre, Jeffrey D. Wallace, Jeremy T. Hatcher, Lon A. Westfall, Niraj V. Nayak

Принадлежит: INGURAN LLC

A multi-channel apparatus for classifying particles according to one or more particle characteristics. The apparatus comprises a plurality of flow cytometry units, each of which is operable to classify particles in a mixture of particles by interrogating a stream of fluid containing the particles with a beam of electromagnetic radiation. The flow cytometry units share an integrated platform comprising at least one of the following: (1) a common supply of particles; (2) a common housing; (3) a common processor for controlling operation of the units; (4) a common processor for receiving and processing information from the units; and (5) a common fluid delivery system. The integrated platform can include a common source of electromagnetic radiation. A method of the invention comprises using a plurality of flow cytometry units sharing the integrated platform to perform a flow kilometric operation, such as analyzing or sorting particles.

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Номер записи: 33

26-09-2013 дата публикации

Sample analyzer and computer program product

Номер: US20130252276A1

Автор: Daigo Fukuma, Noriyuki Narisada, Takaaki Nagai

Принадлежит: Sysmex Corp

A sample analyzer prepares a measurement sample from a blood sample or a body fluid sample which differs from the blood sample; measures the prepared measurement sample; obtains characteristic information representing characteristics of the components in the measurement sample; sets either a blood measurement mode for measuring the blood sample, or a body fluid measurement mode for measuring the body fluid sample as an operating mode; and measures the measurement sample prepared from the blood sample by executing operations in the blood measurement mode when the blood measurement mode has been set, and measuring the measurement sample prepared from the body fluid sample by executing operations in the body fluid measurement mode that differs from the operations in the blood measurement mode when the body fluid measurement mode has been set, is disclosed. A computer program product is also disclosed.

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Номер записи: 34

17-10-2013 дата публикации

Device for performing a blood, cell, and/or pathogen count and methods for use thereof

Номер: US20130273524A1

Автор: Joel R. L. Ehrenkranz

Принадлежит: Joel R. L. Ehrenkranz

Devices and methods for performing a point of care blood, cell, and/or pathogen count or a similar blood test. Disclosed herein are systems that can be used to provide rapid, accurate, affordable laboratory-quality testing at the point of care. The systems described herein are capable of imaging and counting individual cells in a prepared cell sample (e.g., a peripheral blood smear or a blood sample prepared in a microfluidic device) or another prepared cell-containing sample without the need for a microscope or other expensive and cumbersome optics. The systems described herein are designed to eliminate or replace expensive, centralized clinical testing equipment and technical personnel. Such systems may include automated data reporting and decision support.

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Номер записи: 35

28-11-2013 дата публикации

Device for inspecting a biological fluid

Номер: US20130316440A1

Автор: Nelly Rongeat, Patrick Brunel, Philippe Nerin

Принадлежит: CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS, HORIA ABX SA, UNIVERSITE DE LIMOGES

A device for inspecting a biological fluid, including a channel through which the fluid flows, a first inspection module arranged in a first region of the channel, and a second inspection module arranged in a second region of the channel, the device configured to provide a quantity that is representative of output of the second inspection module. The first inspection module is configured to measure at least one electrical property of the fluid passing through the first region. The second inspection module is configured to measure at least one optical property of the fluid passing through the second region. The inspection device also includes a controller connected to the first inspection module and to the second inspection module and configured to control the second inspection module according to the output of the first inspection module.

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Номер записи: 36

30-01-2014 дата публикации

Apparatus for detecting tumor cells

Номер: US20140030799A1

Автор: Chris C. Yu, He Yu, Xuedong Du

Принадлежит: Anpac Bio Medical Science Co Ltd

Among others, the present invention provides apparatus for detecting circulating tumor cells, comprising a system delivery biological subject and a probing and detecting device, wherein the probing and detecting device includes a first micro-device and a first substrate supporting the first micro-device, the first micro-device contacts a biologic material to be detected and is capable of measuring at the microscopic level an electrical, magnetic, electromagnetic, thermal, optical, acoustical, biological, chemical, electro-mechanical, electro-chemical, electro-optical, electro-thermal, electro-chemical-mechanical, bio-chemical, bio-mechanical, bio-optical, bio-thermal, bio-physical, bio-electro-mechanical, bio-electro-chemical, bio-electro-optical, bio-electro-thermal, bio-mechanical-optical, bio-mechanical thermal, bio-thermal-optical, bio-electro-chemical-optical, bio-electro-mechanical-optical, bio-electro-thermal-optical, bio-electro-chemical-mechanical, physical or mechanical property, or a combination thereof, of the biologic subject.

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Номер записи: 37

06-02-2014 дата публикации

Wick wetting for water condensation systems

Номер: US20140033915A1

Автор: Gregory Stephen Lewis, Nathan Michael Kreisberg, Steven Russel Spielman, Susanne Vera Hering

Принадлежит: Aerosol Dynamics Inc

A system and method for particle enlargement with continuously wetted wicks includes a container into which a flow of particle-laden air is introduced in a laminar manner through an inlet and to an outlet. The container has a first section, a second section and a third section though which the particle-laden air flows between the inlet and the outlet. The temperature of the second section is warmer than that of the first section at the inlet and the third section at the outlet. In one embodiment, a continuous wick spanning an interior wall of the first second, second section and third section, said wick being capable of internally transporting liquid water along its length is provided. Alternatively, a wick characterized by a bubble point pressure has one side in contact with air and an opposing side mounted adjacent to the interior wall of a housing with a gap formed between the wick and the housing, wherein the wick is used with a water reservoir such that the pressure difference between the air flow and the water filled gap is less than the bubble point pressure of the wick material.

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Номер записи: 38

13-03-2014 дата публикации

Elemental flow cytometer

Номер: US20140070092A1

Автор: Dmitry R. Bandura, Scott D. Tanner, Vladimir I. Baranov

Принадлежит: PerkinElmer Health Sciences Inc

An elemental flow cytometer includes a device to vaporize, atomize, and ionize material and an introduction system for introducing packets of discrete entities into said device to vaporize, atomize and ionize materials to vaporize, atomize and ionize the entities in the packets. A spectrometer is adapted to individually analyze elemental composition of one or more of the vaporized, atomized and excited or ionized packets.

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Номер записи: 39

13-03-2014 дата публикации

Methods and means for manipulating particles

Номер: US20140073000A1

Автор: Dong Sun, Xiaolin Wang

Принадлежит: City University of Hong Kong CityU

The present invention is concerned with a system for sorting target particles from a flow of particles. The system has a microscope, a light source, a CCD camera, microfluidic chip device with microfluidic channels, a detection apparatus for detecting the target particles with predefined specific features, a response generating apparatus for generating a signal in response to the detection of the target particles, and an optical tweezing system for controlling movement of optical traps, the optical tweezing system is operably linked to the response signal.

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Номер записи: 40

10-04-2014 дата публикации

High pressure sperm sorting and flow cytometer methods

Номер: US20140099628A1

Автор: Kenneth Michael Evans, Ramakrishnan VISHWANATH, Richard W. Lenz, Thomas Boyd Gilligan

Принадлежит: INGURAN LLC

Cell sorting methods that improve sorting efficiency and productivity by elevating sorting pressures and incorporate certain steps to help the cells better survive such elevated pressures. In the case of sperm, sorting the steps of standardizing sperm samples, staining sperm samples in a single step, calibrating a flow cytometer to place sperm in the leading edge of droplets, and changing a catch fluid distance may be incorporated individually, or in combination to help sperm better survive the sex sorting process.

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Номер записи: 41

04-01-2018 дата публикации

DEVICES AND METHODS FOR FRACTIONATED PHOTOACOUSTIC FLOW CYTOMETRY

Номер: US20180000351A1

Автор: Zharov Vladimir Pavlovich

Принадлежит:

A fractionated photoacoustic flow cytometry (PAFC) system and methods for the in vivo detection of target objects in biofluidic systems (e.g., blood, lymph, urine, or cerebrospinal fluid) of a living organism is described. The fractionated system includes a fractionated laser system, a fractionated optical system, a fractionated acoustic system, and combinations thereof. The fractionated laser system includes at least one laser or laser array for pulsing a target object within the circulatory vessel with fractionated focused laser beams. The fractionated optical system separates one or several laser beams into multiple beams in a spatial configuration on the skin above the circulatory vessel of the living organism. The fractionated acoustic system includes multiple focused ultrasound transducers for receiving photoacoustic signals emitted by the target object in response to the fractionated laser beams. The target objects have intrinsic photoacoustic contrast or may be labeled with photoswitchable or spaser-based probes. Fractioned beams may be used also for diagnostics with other spectroscopic methods (e.g., fluorescence, Raman or scattering) and energy sources both coherent and conventional such as lamp and LED in the broad spectral range from 10 Å to 1 cm (e.g., X-ray, UV, visible, NIR or microwaves) in continuous wave and pulse modes. 1. A fractionated photoacoustic flow cytometry system for the in vivo detection of target objects in a biofluid system of a living organism , comprising:a laser system comprising at least one laser comprising at least one wavelength for providing at least one laser beam to at least one target object within the biofluid system;a fractionated optical system configured to separate the at least one laser beam into fractionated laser beams having a spatial configuration on the skin above the biofluid system of the living organism; andan acoustic system comprising at least one focused ultrasound transducer for receiving more than one ...

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Номер записи: 42

04-01-2018 дата публикации

Anti-hsp70 specific chimeric antigen receptors (cars) for cancer immunotherapy

Номер: US20180000914A1

Автор: Alexandre Juillerat, Arvind Rajpal, Barbra Johnson SASU, Julianne Smith, Julien Valton, Philippe Duchateau

Принадлежит: CELLECTIS SA

The present invention relates to Chimeric Antigen Receptors (CAR) that are recombinant chimeric proteins able to redirect immune cell specificity and reactivity toward selected membrane antigens, and more particularly in which extracellular ligand binding is a scFV derived from an anti-HSP70 monoclonal antibody, conferring specific immunity against HSP70 positive cells. The engineered immune cells endowed with such CARs are particularly suited for treating in particular leukemia.

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Номер записи: 43

07-01-2021 дата публикации

Particle separation

Номер: US20210001337A1

Автор: Alexander Govyadinov, Erik D. Torniainen, Pavel Kornilovich, Viktor Shkolnikov

Принадлежит: Hewlett Packard Development Co LP

An example system includes an input channel having a first end and a second end to receive particles through the first end, a separation chamber, at least two output channels, and an integrated pump to facilitate flow through the separation chamber. The separation chamber is in fluid communication with the second end of the input channel. The separation chamber has a passive separation structure, the passive separation structure including an array of columns spaced apart to facilitate separation of particles in a flow based on a size of the particles. Each output channel is in fluid communication with the separation chamber to receive separated particles. The integrated pump is positioned within at least one of the input channel or one of the at least two output channels.

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Номер записи: 44

07-01-2021 дата публикации

Microparticle separation method, microparticle separation program, microparticle separation system

Номер: US20210001338A1

Автор: Kazuya Takahashi, Tatsumi Ito, Yoichi Katsumoto

Принадлежит: Sony Corp

A method of extracting microparticles by detecting target microparticles for extraction in a main flow path which communicates with a pressure chamber, generating for each of the detected target microparticles a change in a negative pressure in the pressure chamber communicating with the main flow path to separate and extract each of the detected target microparticles flowing in the main flow path into the pressure chamber, wherein generating the change of the negative pressure to extract the detected target microparticles comprises generating a negative change in pressure by a different amount in accordance with a separation between the detected target microparticles.

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Номер записи: 45

07-01-2021 дата публикации

SYSTEM AND METHOD FOR DETECTION AND SORTING OF CELLS

Номер: US20210001339A1

Автор: Filatov Zerikhun, Liu Peng

Принадлежит: Microsensor Labs, LLC

A system and method for detection of cells and sorting of cells are disclosed. Target cells, such as circulating tumor cells (CTCs) or antigen-specific antibody producing circulating memory B cells from COVID-19 patients, may be of interest. Magnetic beads may be bound to the target cells. After which, the bead-bound target cells may be identified using an applied magnetic field. In one example, magnetic sensors may be used to detect movement of the bead-bound target cells responsive to an applied magnetic field. In another example, an optical sensor may be used to detect movement of the bead-bound target cells responsive to an applied magnetic field. Further, separate from identification of the target cells, the bead-bound target cells may be sorted using an applied magnetic field. In this way, a magnetic field may be used for target cell identification and target cell sorting in order to detect and collect target cells of interest at the single-cell resolution. 1. An apparatus configured to determining whether a magnetic bead-labeled target cell is present in a fluid , the apparatus comprising:a well configured to house the fluid containing particles and including at least one outlet;at least one magnetic field generator configured to generate a magnetic field to at least a part of the well;one or more sensors configured to generate sensor data; and control the magnetic field generator to generate the magnetic field to the at least a part of the well;', 'identify, based on the sensor data responsive to the magnetic field, the magnetic bead-labeled target cell and an associated location within the well; and', 'control the magnetic field generator, based on the associated location within the well of the magnetic bead-labeled target cell and the at least one outlet, in order to move the magnetic bead-labeled target cell toward the at least one outlet, thereby sorting the magnetic bead-labeled target cell, in order to remove the magnetic bead-labeled target cell from ...

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Номер записи: 46

06-01-2022 дата публикации

Information processing apparatus, information processing method, and program

Номер: US20220003656A1

Автор: Junichiro Enoki, Kenji Yamane, Rei Murata

Принадлежит: Sony Group Corp

To provide an information processing apparatus, at least one non-transitory computer-readable storage medium, and a method which evaluate the appropriateness of a clustering result in consideration of characteristics of multidimensional data to be clustered. An information processing apparatus comprising: at least one hardware processor; and at least one non-transitory computer-readable storage medium storing processor-executable instructions that, when executed by the at least one hardware processor, cause the at least one hardware processor to perform: receiving multidimensional data obtained from a plurality of cells; clustering the multidimensional data to generate clustering results indicating a plurality of clusters including a first cluster and a second cluster that share at least a portion of the multidimensional data; and outputting information representing reliability of the clustering results, wherein the information is indicative of a relationship between the first cluster and the second cluster.

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Номер записи: 47

06-01-2022 дата публикации

METHOD AND SYSTEM FOR CHARACTERIZING PARTICLES USING AN ANGULAR DETECTION IN A FLOW CYTOMETER

Номер: US20220003660A1

Автор: Giesecke-Thiel Claudia, Kage Daniel, Kaiser Toralf, von Volkmann Konrad

Принадлежит:

The invention relates to a method and system for characterizing particles using a flow cytometer comprising detecting radiated light from the particles using two or more detectors positioned to allow for the detection in two or more angular directions and generating a waveform, as a digital representation for the detected radiated light for each of said angulation direction. The waveforms are transformed using one or more basis functions to obtain one or more coefficients characterizing the waveform. The one or more coefficients characterizing the waveform preferably correspond to properties of the particle(s), thereby enabling analysis of physical properties of the particles (such as size, shape, refractive index) or biological properties of the particles (such as cell type, cell cycle state or localization or distribution of molecules within the cell and/or on the cell surface). In preferred embodiments the method and system are used for a label-free sorting of particles, in particular biological cells. 1. A method for characterizing particles using a flow cytometer comprising:a. passing of one or more particles in a fluid stream through a light beam of the flow cytometer,{'b': '3', 'b. detecting radiated light as one or more particles pass through the light beam using two or more detectors positioned to allow for the detection of the radiated light () in two or more angular directions,'}c. generating for each of the angular directions a waveform which is a digital representation of the detected radiated light for said angular direction, andd. transforming each waveform using one or more basis functions and obtaining one or more coefficients characterizing the waveform.2. Method according to claim 1 , wherein the detected radiated light is a forward scatter signal claim 1 , a side scatter signal and/or a fluorescence light and the two or more detectors are positioned to allow for detection of a forward scatter signal claim 1 , a side scatter signal and/or a ...

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Номер записи: 48

07-01-2016 дата публикации

SELECTIVE CELL THERAPY FOR THE TREATMENT OF RENAL FAILURE

Номер: US20160002603A1

Автор: Atala Anthony, Yoo James J.

Принадлежит:

Provided herein are isolated populations of kidney cells harvested from differentiated cells of the kidney, wherein cells have been expanded in vitro. The kidney cells may include peritubular interstitial cells of the kidney, and preferably produce erythropoietin (EPO). The kidney cells may also be selected based upon EPO production. Methods of producing an isolated population of EPO producing cells are also provided, and methods of treating a kidney disease resulting in decreased EPO production in a patient in need thereof are provided, including administering the population to the patient, whereby the cells produce EPO in vivo. 17.-. (canceled)8. A method of producing an isolated population of erythropoietin (EPO) producing cells , said method comprising the steps of:providing differentiated kidney cells; andpassaging said differentiated kidney cells, wherein said cells produce EPO after said passaging;thereby producing an isolated population of EPO producing cells.9. The method of further comprising the step of selecting said differentiated kidney cells for EPO production.10. The method of claim 8 , wherein said passaging step comprises growth of differentiated kidney cells in a medium comprising insulin transferrin selenium (ITS).11. The method of claim 8 , wherein said differentiated kidney cells of said providing step further comprises endothelial cells of the kidney.12. The method of claim 8 , subject to the proviso that said population of EPO producing cells are not transfected with an exogenous DNA encoding a polypeptide.13. The method of claim 9 , wherein said selecting comprises selection based upon density and size.14. The method of claim 9 , wherein said selecting comprises the use of centrifugal gradients.15. The method of claim 8 , wherein said passaging is carried out from 1 to 20 times.16. The method of claim 8 , wherein said passaging is carried out at least 3 times.17. The method of claim 8 , wherein said population produces EPO without ...

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Номер записи: 49

04-01-2018 дата публикации

Cll1-specific multi-chain chimeric antigen receptor

Номер: US20180002427A1

Автор: Alexandre Juillerat, Arvind Rajpal, Barbra Johnson SASU, Julianne Smith, Julien Valton, Philippe Duchateau

Принадлежит: CELLECTIS SA

The present invention relates to a new generation of chimeric antigen receptors (CAR) referred to as multi-chain CARs, which are made specific to the antigen CLL1. Such CARs aim to redirect immune cell specificity and reactivity toward malignant cells expressing the tumor antigen CLL1. The alpha, beta and gamma polypeptides composing these CARs are designed to assemble in juxtamembrane position, which forms flexible architecture closer to natural receptors, that confers optimal signal transduction. The invention encompasses the polynucleotides, vectors encoding said multi-chain CAR and the isolated cells expressing them at their surface, in particularly for their use in immunotherapy. The invention opens the way to efficient adoptive immunotherapy strategies for treating cancer, especially leukemia.

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Номер записи: 50

04-01-2018 дата публикации

mAb-DRIVEN CHIMERIC ANTIGEN RECEPTOR SYSTEMS FOR SORTING/DEPLETING ENGINEERED IMMUNE CELLS

Номер: US20180002435A1

Автор: Duchateau Philippe, Juillerat Alexandre, RAJPAL ARVIND, SASU Barbara Johnson, Valton Julien

Принадлежит:

A polypeptide encoding a chimeric antigen receptor (CAR) comprising at least one extracellular binding domain that comprises a scFv formed by at least a VH chain and a VL chain specific to an antigen, wherein said extracellular binding domain comprises at least one mAb-specific epitope. 1. A polypeptide encoding a chimeric antigen receptor (CAR) comprising at least one extracellular binding domain that comprises a scFv formed by at least a VH chain and a VL chain specific to an antigen , wherein said extracellular binding domain comprises at least one mAb-specific epitope.2. The polypeptide according to claim 1 , wherein said mAb-specific epitope is located between the VH and VL chains.3. The polypeptide according to claim 1 , wherein said VH and VL chains claim 1 , and mAb specific-epitope are bound together by at least one linker and to the transmembrane domain of said CAR by a hinge.4. The polypeptide according to claim 3 , wherein the mAb-specific epitope is joined to the VH and VL chains by two linkers.5. The polypeptide according to claim 1 , wherein the mAb-specific epitope is an epitope to be bound by an epitope-specific mAb for in vitro cell sorting and/or in vivo cell depletion of T cells expressing a CAR comprising such epitope.6. The polypeptide according to claim 1 , wherein the polypeptide comprises one extracellular binding domain claim 1 , wherein said extracellular binding domain further comprises a hinge claim 1 , and said polypeptide further comprisesa transmembrane domain, and,an intracellular domain.7. The polypeptide according to claim 1 , wherein the extracellular binding domain comprises 1 claim 1 , 2 claim 1 , 3 claim 1 , 4 claim 1 , 5 claim 1 , 6 claim 1 , 7 claim 1 , 8 claim 1 , 9 or 10 mAb-specific epitopes.8. The polypeptide according to claim 1 , wherein the extracellular binding domain comprises 1 claim 1 , 2 claim 1 , 3 or claim 1 , 4 mAb-specific epitopes.9. The polypeptide according to claim 1 , wherein the extracellular binding ...

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Номер записи: 51

04-01-2018 дата публикации

PURIFICATION OF GERM STEM CELLS BY TARGETING MRP9

Номер: US20180002662A1

Автор: Bonkowski Michael S., Schultz Michael B., SINCLAIR DAVID A.

Принадлежит: President and Fellows of Harvard College

Provided herein are methods and compositions for the purification and detection of germ stem cells (e.g., oogonial stem cells) based on expression of MRP9. 1. A method for purifying a germ stem cell (GSC) , the method comprising:(a) contacting a sample comprising the GSC with an anti-MRP9 antibody that specifically binds to MRP9 and that does not specifically bind to DDX4;(b) incubating the sample under conditions such that the anti-MRP9 antibody forms a complex with an MRP9 protein expressed on the surface of the GSC; and(c) separating the GSC from other material present in the sample.2. The method of claim 1 , wherein the GSC is a oogonial stem cell (OSC).3. The method of claim 2 , wherein the sample is an ovarian tissue sample.4. The method of claim 3 , further comprising the step of obtaining the ovarian tissue sample from a subject.5. The method of claim 1 , wherein the GSC is a spermatogonial stem cell (SSC).6. The method of any one of to claim 1 , wherein the GSC is a human GSC.7. The method of any one of to claim 1 , wherein the anti-MRP9 antibody is monoclonal.8. The method of any one of to claim 1 , wherein the anti-MRP9 antibody is polyclonal.9. The method of any one of to claim 1 , wherein the anti-MRP9 antibody specifically binds to an extracellular region of MRP9.10. The method of any one of to claim 1 , wherein the anti-MRP9 antibody does not specifically bind to an epitope having a sequence of APNPVDD.11. The method of any one of to claim 1 , wherein the anti-MRP9 antibody specifically binds to an extracellular region of MRP9 having a sequence selected from the group consisting of SEQ ID NOs 5-20.12. The method of any one of to claim 1 , wherein the anti-MRP9 antibody specifically bind to an extracellular region of MRP9 having a sequence selected from the group consisting of SEQ ID NOs 6-12.13. The method of any one of to claim 1 , wherein the GSC is separated from other material present in the sample in step (c) by fluorescent activated cell sorting ...

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Номер записи: 52

04-01-2018 дата публикации

METHOD FOR ASSISTING IN EVALUATING PLURIPOTENT STEM CELL WITHOUT STAINS, STORAGE MEDIUM, AND OPERATION DEVICE

Номер: US20180002670A1

Автор: Ishiwata Hiroshi

Принадлежит: OLYMPUS CORPORATION

An operation device includes: a communication unit that receives signals of images of a biological sample and outputs data to a display medium, the images being captured by a microscope that converts a phase distribution into an image intensity distribution; and a calculator that calculates a first phase distribution of the biological sample from the image signals. The calculator extracts a region having a phase amount that is not less than a specified phase amount from the first phase distribution, and generates evaluation information by using the region having a phase amount that is not less than the specified phase amount, that is an indicator used when a user evaluates a state of the biological sample; and the communication unit outputs the evaluation information to the display medium. 1. A method for assisting in evaluating a pluripotent stem cell without stains , the method comprising:calculating a first phase distribution of a biological sample from signals of images of the biological sample, the images being captured by a microscope that converts a phase distribution into an image intensity distribution;extracting a region having a phase amount that is not less than a specified phase amount from the first phase distribution; andgenerating evaluation information by using the region having a phase amount that is not less than the specified phase amount, that is an indicator used when a user evaluates a state of the biological sample, and presenting the evaluation information, whereinthe specified phase amount is a phase amount of a mitochondrion in a somatic cell.2. The method for assisting in evaluating a pluripotent stem cell without stains according to claim 1 , whereinthe evaluation information is generated by distinguishing the region having a phase amount that is not less than the specified phase amount from another region in an image of the first phase distribution.3. The method for assisting in evaluating a pluripotent stem cell without stains ...

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Номер записи: 53

05-01-2017 дата публикации

COMPUTER-IMPLEMENTED METHOD OF ANALYSING DATA FROM MEASURED VALUES OF CHARACTERISTICS OF OBJECTS IN SAMPLES

Номер: US20170003212A1

Автор: BUYDENS Lutgarde, HILVERING Bart, JANSE Jeroen, KOENDERMAN Leo, VAN DEN BRINK Oscar

Принадлежит:

The invention relates to a computer implemented method of analysing data comprising measured values of characteristics of objects in samples, the data comprising —a first set of data (X) with measured values of characteristics of objects in reference samples; —a test set of data (X) with measured values of the characteristics of objects in a test sample; characterised by the method comprising; —fitting a control model to the first set of data to determine control loadings (P) each representing an independent correlation between characteristics; —projecting the first set of data (X) onto the control loadings (P) for determining a first set control scores (T) and determining one or more confidence intervals for the first set of control scores (T); —projecting the test data onto the control loadings (P) for determining test control scores; —determining if the test control scores are within one or more the confidence intervals. 117.-. (canceled)19. Computer implemented method according to claim 18 , wherein the step of fitting a control model comprises using the computer to conduct a principal component analysis to determine the control loadings (P).20. Computer implemented method according to claim 18 , wherein the first set of data (X) is centered per sample by using a computer for determining per reference sample (i) the mean value (m) of each characteristic and subtracting for each reference sample (i) the corresponding mean value (m) of the characteristic from the measured value in the first set of data (X) of the corresponding characteristic before fitting the control model.21. Computer implemented method according to claim 18 , wherein the test set of data (X) is centered per sample by using a computer for determining per test sample (i) the mean value of each characteristic and subtracting for each test sample (i) the corresponding mean value of the characteristic from the measured value in the test set of data of the corresponding characteristic before ...

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Номер записи: 54

05-01-2017 дата публикации

Analysis Device and Analysis Method

Номер: US20170003213A1

Автор: HASEGAWA YUICHI, ITONAGA Makoto, ONO Masayuki, TSUJITA Koji, YAGYU Shingo

Принадлежит:

An analysis device optically scans a surface of a substrate to which analytes and particles for labeling the analytes are fixed, detects a pulse wave included in a detection signal obtained from an optical scanning unit when the optical scanning unit scans the substrate, and counts the analytes and determines that the analyte count is one when two pulse waves are detected consecutively each having pulse width less than first reference value determined depending on first pulse width in the detection signal when the optical scanning unit scans a plurality of particles adjacent to each other. 1. An analysis device comprising:an optical scanning unit configured to optically scan a surface of a substrate to which analytes and particles for labeling the analytes are fixed;a pulse detector configured to detect a pulse wave and a pulse width of the pulse wave included in a detection signal obtained from the optical scanning unit when the optical scanning unit scans the substrate; anda counting unit configured to count the analytes and determine that an analyte count is one when the pulse detector consecutively detects two pulse waves each having a pulse width less than a first reference value.2. The analysis device according to claim 1 , wherein the counting unit determines that the analyte count is one when the pulse detector detects a pulse wave having a pulse width greater than or equal to the first reference value and less than a second reference value.3. The analysis device according to claim 1 , wherein claim 1 , when the pulse detector detects a first pulse wave having a pulse width less than the first reference value claim 1 , detects a second pulse wave after the first pulse wave claim 1 , and a pulse interval between the first pulse wave and the second pulse wave is greater than or equal to a third reference value claim 1 , the counting unit does not implement counting processing with regard to both the first pulse wave and the second pulse wave.4. The analysis ...

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Номер записи: 55

05-01-2017 дата публикации

PARTICLE MEASURING DEVICE

Номер: US20170003221A1

Автор: ASANO Takamasa, HASEGAWA Yoshiki, KOIZUMI Kazuhiro, TAKEDA Naoki

Принадлежит: FUJI ELECTRIC CO., LTD.

A particle measuring device includes: an optical resonator that reflects laser light back and forth between two facing reflective mirrors in order to amplify an energy of that laser light and form resonant laser light; a particle transport unit that transports particles in an aerosol to be measured across a beam path of the resonant laser light; a scattered light receiving unit that receives scattered light produced when the particles in the aerosol are irradiated by the resonant laser light; and a processor that receives light reception signals from the scattered light receiving unit, wherein the processor outputs light reception pulses according to the light reception signals and calculates time intervals between the light reception pulses that are temporally adjacent. 1. A particle measuring device for measuring particles in an aerosol , comprising:an optical resonator that causes laser light to travel back and forth between two opposing reflective mirrors in order to amplify an energy of the laser light and form resonant laser light;a particle transport unit configured to transport the particles in the aerosol across a beam path of the resonant laser light so as to generate a stream of the particles crossing the beam path;a scattered light receiving unit configured to receive scattered light that is produced when the particles in the aerosol are irradiated by the resonant laser light, and output a light reception signal in accordance with the received scattered light for each scattering event; anda processor that receives the light reception signal from the scattered light receiving unit for each event of the reception of the scattered light,wherein the processor outputs a light reception pulse in accordance with each light reception signal from the scattered light receiving unit, and derives time intervals between light reception pulses that are temporally adjacent.2. The particle measuring device according to claim 1 , wherein the processor further derives a ...

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Номер записи: 56

05-01-2017 дата публикации

Compositions and methods for screening t cells with antigens for specific populations

Номер: US20170003288A1

Автор: James R. Heath, Songming PENG

Принадлежит: California Institute of Technology CalTech

Compositions and methods for isolating patient-derived antigen-specific T cells include an antigen complex having a polynucleotide barcoded nanoparticle sorting agent complexed with a peptide-loaded streptavidin major histocompatability complex (MHC) tetramer, the barcoding technology allowing for high fidelity screening of a library of the antigen complexes to readily isolate and identify antigen-specific T cells.

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Номер записи: 57

07-01-2016 дата публикации

PHOTODETECTION DEVICE

Номер: US20160003727A1

Автор: Tanaka Masaki

Принадлежит: SHARP KABUSHIKI KAISHA

A photodetection device has an optical module () that includes a light source (), an excitation optical system, and a detection optical system and that two-dimensionally and relatively scans a transparent stage () in a first sampling direction and a second sampling direction intersecting the first sampling direction. A scan length in the first sampling direction is longer than a scan length in the second sampling direction. A data sampling unit in the detection optical system performs sampling for a distance of a second sampling interval during scanning in the second sampling direction, and performs sampling for a distance of a first sampling interval shorter than the distance of the second sampling interval during scanning in the first sampling direction. An aperture () of the excitation optical system sets a size in the first sampling direction of a spot shape of excitation light from the light source () to be smaller than a size in the second sampling direction. 1. A photodetection device comprising:a light-transmitting transparent stage on which a detection object is placed;an excitation optical system that irradiates the detection object with excitation light emitted from a light source;a detection optical system that detects light emitted from a detection surface of the detection object placed on the transparent stage by irradiation with the excitation light;a data sampling unit included in the detection optical system to sample an intensity of the detected light at a predetermined set interval; andan optical module that includes the light source, the excitation optical system, and the detection optical system and that two-dimensionally and relatively scans the transparent stage in a first sampling direction of the data sampling unit and a second sampling direction intersecting the first sampling direction,wherein a scan length in the first sampling direction in the optical module is longer than a scan length in the second sampling direction,wherein the data ...

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Номер записи: 58

07-01-2016 дата публикации

METHOD OF AND APPARATUS FOR ASCERTAINING THE SIZE OF PARTICLES

Номер: US20160003728A1

Автор: Hardalupas Yannis

Принадлежит:

A method of ascertaining the size of small particles is disclosed. The method includes the steps of: a) intersecting at least two light beams at an intersection volume; b) sensing at each of a plurality of sensing positions angularly displaced from one another light scattered by a particle substantially in the intersection volume, and producing respective output signals indicative of the sensed light; c) ascertaining the phase difference between one of the signals and each other of the signals to give a measured indication of the variation of phase difference with angular displacement; and d) comparing the measured indication with at least one known indication of the variation of phase difference with angle for a known particle size and thereby determining the size of the particle substantially in the intersection volume. 1. A method of ascertaining the size of small particles , the method including the steps of:a) intersecting at least two light beams at an intersection volume;b) sensing at each of a plurality of sensing positions angularly displaced from one another light scattered by a particle substantially in the intersection volume, and producing respective output signals indicative of the sensed light;c) ascertaining the phase difference between one of the signals and each other of the signals to give a measured indication of the variation of phase difference with angular displacement; andd) comparing the measured indication with at least one known indication of the variation of phase difference with angle for a known particle size and thereby determining the size of the particle substantially in the intersection volume.2. A method according to claim 1 , wherein the measured indication of the variation of phase difference with angular displacement includes an indication of the angular position of transitions between local maxima and minima of the phase difference.3. A method according to claim 2 , wherein the measured indication includes information ...

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Номер записи: 59

07-01-2016 дата публикации

FLUIDIC FLOW CYTOMETRY DEVICES AND PARTICLE SENSING BASED ON SIGNAL-ENCODING

Номер: US20160003729A1

Автор: Chen Chun Hao Randy, Cho Sung Hwan, Lo Yu-Hwa, Tsai Shang-Feng

Принадлежит:

Microfluidic devices, systems and techniques in connection with particle sorting in liquid, including cytometry devices and techniques and applications in chemical or biological testing and diagnostic measurements. 1. A particle sorter for sorting particles in a fluid , comprising:a structure having an input channel connected at an actuation area to a plurality of output channels, wherein the particles in the fluid flow through the input channel to the actuation area, and each particle travels from the actuation area to one of the plurality of output channels, anda piezoelectric actuator for causing a flow disturbance in the actuation area in response to a control signal, wherein the flow disturbance operates to direct a particle along a trajectory to one of the plurality of output channels which is different than the output channel to which the particle would travel without the flow disturbance.2. The particle sorter of claim 1 , wherein the structure includes at least one of a polymer substrate claim 1 , a polydimethylsiloxine (PDMS) substrate claim 1 , or a glass substrate.3. The particle sorter of claim 2 , wherein the piezoelectric actuator is permanently bonded via UV ozone treatment to the PDMS substrate.4. The particle sorter of claim 1 , wherein the piezoelectric actuator is integrated with the structure.5. The particle sorter of claim 1 , further comprising a driver for generating the control signal.6. The particle sorter of claim 1 , wherein the control signal is a voltage signal having a controlled magnitude and frequency.7. The particle sorter of claim 1 , wherein the detection unit comprises a bank of filters for detecting a signal from the particle.8. The particle sorter of claim 1 , wherein the piezoelectric actuator includes a contact layer for coupling the piezoelectric actuator to the structure claim 1 , and further includes a piezoelectric layer for generating a signal to cause the flow disturbance in response to the control signal.9. The ...

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Номер записи: 60

07-01-2016 дата публикации

FLOW CYTOMETRY SYSTEM AND METHOD

Номер: US20160003730A1

Автор: Dekker Ronald, Hoekman Marcel, Hosseini Seyed Naser, Schreuder Frederik

Принадлежит:

A flow cytometry system having a flow channel defined through the thickness of a substrate is disclosed. Fluid flowing through the flow channel is illuminated by a first plurality of surface waveguides that are arranged around the flow channel in a first plane, while a second plurality of surface waveguides arranged around the flow channel in a second plane receive light after it has interacted with the fluid. The illumination pattern provided to the fluid is controlled by controlling the phase of the light in the first plurality of surface waveguides. As a result, the fluid is illuminated with light that is uniform and has a low coefficient of variation, improving the ability to distinguish and quantify characteristics of the fluid, such as cell count, DNA content, and the like. 1. An apparatus comprising:a substrate that defines a first plane, the substrate comprising a flow channel that is operative for conveying fluid along a first direction that is substantially orthogonal to the first plane, the flow channel being located within a first region of the substrate;a first surface waveguide that is optically coupled with the flow channel, the first surface waveguide being located in a second plane within the first region, wherein the second plane is substantially parallel with the first plane; anda second surface waveguide that is optically coupled with the flow channel in the first region, the second surface waveguide being located in a third plane within the first region, wherein the third plane is substantially parallel with the second plane.2. The apparatus of claim 1 , wherein the second plane and the third plane are the same plane.3. The apparatus of claim 1 , further comprising:a first plurality of surface waveguides that includes the first surface waveguide, each of the first plurality of surface waveguides being located in the second plane in the first region and being optically coupled with the flow channel; anda second plurality of surface waveguides ...

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Номер записи: 61

04-01-2018 дата публикации

Method for calibrating investigated volume for light sheet based nanoparticle tracking and counting apparatus

Номер: US20180003610A1

Автор: Tatarkiewicz Jan J.

Принадлежит:

A method for calibrating a dark field microcopy setup is disclosed. The method includes preparing a plurality of particle samples, each with a known concentration and particle size, the plurality having more than one particle size and, optionally, more than one refractive index and more than one diluent. For each sample in the plurality, the sample is measured in the setup and the scattered light intensity and number of particles is measured. From this data, a relationship between the scattered light intensity, particle size and calibrated investigated volume can be determined. The calibrated investigated volume is used to obtain the proper particle size distribution in a given diluent. 1. A system for determining the particle size distribution of a colloid , the system comprising:a light source constructed to emit a beam of electromagnetic radiation at a specimen chamber, the chamber is constructed to hold the colloid and to allow a portion of the beam to scatter from particles contained in the colloid;a sensor positioned to observe the scattered portion of the beam, wherein the sensor is adapted to detect the electromagnetic radiation;a database configured to store a predetermined relationship between a scattered light intensity, a particle size and a calibrated investigated volume; a. activating the light source;', 'b. obtaining a series of images from the sensor;', i. measuring the number of particles in the images;', 'ii. measuring the scattered light intensity for the particles in the images individually;', 'iii. based on steps (c)(i) and (ii), determining the calibrated investigated volume based on the predetermined relationship;, 'c. determining the size of the particles in the images by tracking Brownian motion, for each size;'}, 'd. calculating the particle size distribution of the colloid based on the measured number of particles from step (c)(i) and the determined calibrated investigated volume from step (c)(iii)., 'a processor connected to the light ...

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Номер записи: 62

04-01-2018 дата публикации

Microfluidic Sensing

Номер: US20180003611A1

Автор: DOMINGUE Chantelle, Giri Manish, McGuinness Nick, Sells Jeremy

Принадлежит: Hewlett-Packard Development Company, L.P.

A device including a microfluidic channel structure formed on a substrate and including a first channel and a fluid actuator within the microfluidic channel structure. A sense region within the first channel is to receive a fluid flow of target biologic particles for counting in a single file pattern, with the sense region having a volume on a same order of magnitude as a volume of a single one of the target biologic particles. 1. A biologic test chip comprising:a substrate;a microfluidic channel structure formed on the substrate and including a first channel;a fluid actuator within the microfluidic channel structure.a sense region within the first channel to receive a fluid flow of biologic particles on a one-at-a-time basis via operation of the fluid actuator, the sense region having a volume on a same order of magnitude as a volume of a single respective one of the biologic particles.2. The chip of claim 1 , wherein the sense region operates according to a volume fraction in which a ratio of the volume of each single biologic particle relative to the volume of the sense region is on an order of tenths.3. The chip of claim 2 , comprising:at least one impedance sensor generally coextensive within the sense region to count biologic particles passing through the sense region.4. The chip of claim 3 , wherein the biologic particles are subject to a dilution factor on the order of tens.5. The chip of claim 4 , wherein the channel structure provides a non-uniform flow portion to align the biological particles into a single file flow pattern through the sense region claim 4 , the non-uniform flow portion including at least one of:an exclusion structure upstream from the sense region to exclude biologic particles larger than the volume of the sense region; andan inlet including a progressively narrowing cross-sectional area in the downstream orientation.6. The chip of claim 5 , wherein the first channel generally defines a first cross-sectional area and the first channel ...

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Номер записи: 63

04-01-2018 дата публикации

Particulate matter detector

Номер: US20180003612A1

Автор: Barrett E. Cole, Robert P. COSTELLO

Принадлежит: Honeywell International Inc

Devices and methods for detecting particulate matter are described herein. One device includes a laser, a reflector, an ellipsoidal reflector, and a detector, wherein the laser is configured to emit a beam, the reflector is configured to reflect the beam toward the ellipsoidal reflector, and the ellipsoidal reflector has a first focal region located on a path of the reflected beam, and a second focal region located at a surface of the detector.

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Номер записи: 64

04-01-2018 дата публикации

Phenotypic High-Content Assay to Evaluate Drugs

Номер: US20180003613A1

Автор: Conn P. Michael

Принадлежит:

The present invention includes a high throughput screen for an active agent for the treatment of comprising: plating cells at least one pathophysiologically relevant mislocated mutant form of a peroxisomal enzyme; adding a control and compound to each plate from a library of compounds; fixing the cells; contacting the cells with an agent that detects the mislocated mutant form of a peroxisomal enzyme; and imaging the cells in the wells. 1. A method of determining the effectiveness of one or more drug candidates to change the intracellular localization of a target molecule , the method comprising:(a) incubating the one or more drug candidates with a first subset of the cells, and a control agent with a second subset of the cells;(b) fixing and staining the first and second subset of cells, wherein the stain detects the target molecule;(c) generating images of the first and second subset of cells with a camera;(d) measuring the difference in the intracellular localization of the target molecule in the first as compared to a second subset of cells; and(e) determining if the drug candidate modifies the localization of the intracellular localization of the target protein, wherein if the candidate drug modifies the intracellular localization of the target protein when compared to the placebo it is an effective drug candidate.4. The method of claim 1 , further comprising the step of determining cell count claim 1 , nuclear intensity claim 1 , morphology and condensation.5. The method of claim 1 , wherein the localization changes from the cytosol or mitochondria to a peroxisome.6. The method of claim 1 , wherein a candidate drug is selected from at least one of 26-Deoxymonensin B claim 1 , nigericin claim 1 , salinomycin claim 1 , or active derivatives thereof.7. A method of determining the effectiveness of one or more candidate pharmacoperones to treat and/or prevent protein misfolding claim 1 , the method comprising:(a) incubating the one or more candidate pharmacoperones ...

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Номер записи: 65

04-01-2018 дата публикации

MULTI-THREADED FLUID PARAMETER SIGNAL PROCESSING

Номер: US20180003614A1

Автор: Giri Manish, Majeeth Saleem Jaffer, Sait M. A. Shameed

Принадлежит: Hewlett-Packard Development Company, L.P.

A data receiver thread is continuously executed to receive in which signals indicating a fluid parameter. A predetermined time quantity of the signals is repeatedly buffered. Upon completion of the buffering of each predetermined time quantity of the signals, a data processing thread is initiated that executes on the just completed buffered predetermined time quantity of signals. Upon completion of each data processing thread, data from the just completed data processing thread is passed to a data plotting thread. Results of the data plotting thread are displayed on a portable electronic device while the data receiver thread is being executed. 1. A method comprising:(a) outputting signals from a sensor that indicate a fluid parameter;(b) continuously executing a data receiver thread in which the signals indicating the fluid parameter are received;(c) repeatedly buffering a predetermined time quantity of the signals;(d) upon completion of the buffering of each predetermined time quantity of the signals, initiating a data processing thread that executes on the just completed buffered predetermined time quantity of signals;(e) upon completion of each data processing thread, passing data from the just completed data processing thread to a data plotting thread; and(f) displaying results of the data plotting thread on a portable electronic device while the data receiver thread is being executed.2. The method of claim 1 , wherein the data receiver thread receives the signals at a rate of at least 500 kHz.3. The method of further comprising:transmitting first data from the data processing thread across a wide area network to a remote server;receiving second data from the remote server across the wide area network, the second data comprising results of an analysis of the first data by the remote server; andpresenting the second data on the portable electronic device.4. The method of claim 1 , wherein the fluid comprises blood.5. The method of claim 1 , wherein the data ...

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Номер записи: 66

03-01-2019 дата публикации

DOWNHOLE LOCAL SOLID PARTICLES COUNTING PROBE, PRODUCTION LOGGING TOOL COMPRISING THE SAME AND SAND ENTRY INVESTIGATION METHOD FOR HYDROCARBON WELLS

Номер: US20190003303A1

Автор: ABBASSI Linda, Donzier Eric, TAVERNIER Emmanuel

Принадлежит: Openfield SA

A downhole local solid particles counting probe () for counting solid particles () in a fluid () present in a hydrocarbon well in production comprising: 1. A downhole local solid particles counting probe for counting solid particles in a fluid present in a hydrocarbon well in production comprising:an elongated and flexible protective tube defining an internal cavity terminating by a membrane wall defining a tip, the protective tube and the membrane wall isolating the internal cavity from the fluid of the hydrocarbon well, the protective tube and membrane wall are made of metal or metal alloy and have a thickness such as to resist to a downhole hydrocarbon well pressure;a passive acoustic sensor mounted inside the internal cavity, the passive acoustic sensor having a front side mechanically coupled on the membrane wall of the tip;wherein:a characteristic dimension of the passive acoustic sensor is similar to solid particles average characteristic dimension, ranging from 0.5 mm to 1.5 mm, and a characteristic dimension of the membrane wall defining the tip ranges from 1 mm to 2 mm;the passive acoustic sensor is arranged to detect acoustic waves generated by solid particles impacting the membrane wall defining the tip so as to resolve an individual impact from a single solid particle and to produce a signal representative of a count of solid particles.2. The probe of claim 1 , wherein the internal cavity is under a pressure ranging from 0 to 4 atm.3. The probe according to claim 1 , wherein the passive acoustic sensor has a disk shape claim 1 , and the protective tube claim 1 , internal cavity and membrane wall have a cylindrical shape.4. The probe according to claim 1 , wherein the passive acoustic sensor is a piezoelectric ceramic.5. The probe according to claim 1 , wherein the protective tube and the membrane wall are made of austenite nickel-chromium-based super-alloys.6. The probe according to claim 4 , wherein the piezoelectric ceramic comprises metallization ...

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Номер записи: 67

02-01-2020 дата публикации

LASER SENSOR MODULE FOR PARTICLE DETECTION WITH OFFSET BEAM

Номер: US20200003673A1

Автор: Jutte Petrus Theodorus, SPRUIT Johannes Hendrikus Maria, VAN DER LEE ALEXANDER MARC

Принадлежит:

A laser sensor module for detecting a particle density of particles, which includes: a laser; a detector; and a mirror. The laser is arranged to emit a laser beam to the mirror. A movement of the mirror is arranged to redirect the laser beam. The laser beam is displaced with respect to a rotation axis of the mirror such that a focus region of the laser beam is moving with a velocity having components normal and parallel to the optical axis of the redirected laser beam such that an angle between the parallel and the normal velocity component is at least a threshold angle of 2°. The detector is arranged to determine a self mixing interference signal of an optical wave within a laser cavity of the laser, the self mixing interference signal being generated by laser light of the laser beam reflected by at least one of the particles. 1. A laser sensor module for detecting a particle density of particles with a size of less than 20 μm , wherein the laser sensor module comprises:a laser;a detector; anda mirror rotatable about a rotation axis,wherein the laser beam is focused to a focus region,wherein the laser is arranged to emit a laser beam to the mirror,wherein a movement of the mirror is arranged to dynamically redirect the laser beam,wherein a direction of the redirected laser beam defines an optical axis,wherein the laser beam is displaced with respect to the rotation axis of the mirror such that the focus region of the laser beam is moving with a velocity comprising components normal and parallel to the optical axis of the redirected laser beam such that an angle α between the parallel velocity component with the normal velocity component is at least a threshold angle of 2°, andwherein the detector is arranged to determine a self mixing interference signal of an optical wave within a laser cavity of the laser, the self mixing interference signal being generated by laser light of the laser beam reflected by at least one of the particles.2. The laser sensor module ...

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Номер записи: 68

02-01-2020 дата публикации

PERSONAL AIR QUALITY MONITORING SYSTEM

Номер: US20200003674A1

Автор: GIANDOMENICO Adam, PARISEAU David

Принадлежит:

An airborne, gas, or liquid particle sensor with multiple particle sensor blocks in a single particle counter. Each sensor would sample a portion of the incoming airstream, or possibly a separate airstream. The various counters could be used separately or in concert. 1. A personal particle counter apparatus , comprising:a power source in a device housing;an air intake and outlet fluidly connected to at least one chamber in the device housing;at least one light source that passes through the at least one chamber;at least one photo-detector within the at least one chamber, the at least one photo-detector configured to detect airborne particulates passing through the beam in air traversing from the air intake to the outlet;at least one amplifier in communication with the at least one photo-detector, the at least one amplifier configured to convert signals from the at least one photo-detector into amplified electrical pulses;at least one threshold comparator configured to process the amplified electrical pulses from the at least one amplifier and produces at least one output based on any pulse above a threshold pulse height that is a peak voltage of the amplified electrical pulse, the threshold associated with at least one particulate size channel;at least one microcontroller configured to process the at least one output from the at least one threshold comparator related to the at least one particulate size channel to count particles that pass through the chamber;and at least one output mechanism or storage device configured to communicate with with the at least one microcontroller to perform, at least one of, store and communicate the collected information.2. The personal particle counter apparatus of wherein the apparatus further comprises:an air flow device within the device housing.3. The personal particle counter apparatus of wherein the airflow device further comprises:a fan or blower configured to draw or push air through a beam of light in the chamber.4. The ...

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Номер записи: 69

02-01-2020 дата публикации

Methods for predicting overall and progression free survival in subjects having cancer using circulating cancer associated macrophage-like cells (camls)

Номер: US20200003781A1

Автор: Cha-Mei Tang, Daniel Adams

Принадлежит: Creatv Microtech Inc

Means for predicting overall survival (OS) and progression free survival (PFS) of subjects having cancer are disclosed, where the predictions are based on the number arid size of circulating cancer associated macrophage-like cells (CAMLs) found in a biological sample, such as blood, from the subject.

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Номер записи: 70

07-01-2021 дата публикации

Information processing apparatus and method and system for particle simulation

Номер: US20210003493A1

Автор: Kohei Hatanaka, Masaki Kazama, Tamon SUWA

Принадлежит: Fujitsu Ltd

Technique includes acquiring first contact data of first time, associated with first particle in first region; calculating first position data on particles in the first region at second time, and receiving second position data on particles in second region at the second time; detecting second particle being in contact with the first particle and in the first region at the first time and being in the first region at the second time; copying, when the first and second particles are in contact at the second time, displacement of the second particle from the first contact data to second contact data of the second time; detecting third particle being in the first or second region at the second time and in contact with the first particle; and copying, when the third particle is listed in the first contact data, displacement of the third particle to the second contact data therefrom.

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Номер записи: 71

07-01-2021 дата публикации

Fine particle measurement device

Номер: US20210003494A1

Автор: Akinori Kimura, Asako MOTOMURA, Hiroshi Suganuma, Yoko Sugiyama

Принадлежит: Sumitomo Electric Industries Ltd

A fine particle measurement device includes a support stand (20) that has a groove (F) extending in a predetermined direction and is configured to support in the groove an observation container (10), which has an elongate shape and accommodates a liquid sample containing a fine particle therein such that an extending direction of the groove (F) coincides with a longitudinal direction of the observation container (10); and an imaging unit (40) that is configured to capture an image of the fine particle in the observation container (10) at a position where the support stand is out of a field of view, the observation container (10) being supported by the support stand (20).

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Номер записи: 72

07-01-2021 дата публикации

Method and apparatus to collect, fractionate, and classify airborne particles within specified volumetric regions

Номер: US20210003495A1

Автор: Wyatt Philip Joseph

Принадлежит:

A searching procedure based on the detection and separation by specific multiangle scattered light properties of a small set of particles sought from a sample containing far greater concentrations of irrelevant particles is presented whereby all the particulate content of a volumetric fraction of the air of a targeted facility is captured and the particles of potential importance are separated therefrom. The unique size ranges, multiangle light scattering characteristics, and physical properties associated with specific particulates sought (such as bacteria or spores from spore-forming bacteria or asbestos particles, for example), once extracted from the total populations collected, are then processed for further study/classification/actions, as required. For the case of airborne bacteria, such processes could be expected to reduce the incidence of hospital acquired infections, by providing an early warning of their presence and capturing specific exemplars thereof for future analyses. Similarly, the early detection of a bioterrorist attack in progress or the presence of dangerous airborne contaminants, such as asbestos fibers, will have long serving benefits. 120-. (canceled)21: A method to search for , isolate , find , and retrieve vanishingly small populations of a specific class of airborne particles that may be present in a selected region of air containing far greater populations of unimportant particles of varying size and composition , comprising the steps ofa. Defining the specific class of airborne particles sought;b. Collecting all particles present within said selected region of air into a liquid of relatively small volume;c. Fractionating said collected liquid-borne particles by size or other means;d. Classifying and/or identifying said fractionated and collected particles from multiangle measurement of light scattered therefrom;e. Isolating for further study, analysis and/or identification the specific fractions of said collected particles among the ...

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Номер записи: 73

13-01-2022 дата публикации

MICROBIAL CYTOMETRIC MOCK COMMUNITIES AND USE THEREOF AS STANDARD IN FLOW CYTOMETRY

Номер: US20220010351A1

Автор: CICHOCKI Nicolas, HÜBSCHMANN Thomas, MÜLLER Susann, OVERMANN Jörg

Принадлежит:

The present invention is directed to a microbial Cytometric Mock Community for use in flow cytometric analysis, the microbial Cytometric Mock Community comprising or consisting of cells of at least three different microbial species in a pre-defined ratio, wherein the at least three different microbial species are selected such that, when measured using flow cytometry, the specific gate pattern of each microbial species differs significantly from the specific gate pattern of the other microbial species of the microbial Cytometric Mock Community, preferably the at least three different microbial species differ in relative DNA content, relative genomic GC-content, relative cell size, Gram +/− affiliation and/or capacity to form spores. The microbial Cytometric Mock Community shall serve as standardization means that will help ecologists, microbiologists, molecular biologists and flow cytometrists to work on a standardized basis to allow comparison and exchange of data. 1. Microbial Cytometric Mock Community for use in flow cytometric analysis , the microbial Cytometric Mock Community comprising or consisting of cells of at least three different microbial species in a pre-defined ratio , wherein the at least three different microbial species are selected such that , when measured using flow cytometry , the specific gate pattern of each microbial species differs significantly from the specific gate pattern of the other microbial species of the microbial Cytometric Mock Community , preferably the at least three different microbial species differ in overall DNA content , relative genomic GC-content , average cell size , Gram +/− affiliation and/or capacity to form spores.2. Microbial Cytometric Mock Community of claim 1 , wherein the at least three different microbial species comprise or consist of species derived from archaea claim 1 , bacteria claim 1 , fungi claim 1 , protozoa and algae claim 1 , preferably derived from bacterial species.3. Microbial Cytometric Mock ...

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Номер записи: 74

03-01-2019 дата публикации

Particle Detection Methods and Systems for Practicing Same

Номер: US20190003952A1

Автор: Hulett, III Frederic M., Shah Amish P.

Принадлежит:

Aspects of the present disclosure include methods for detecting events in a flow cytometer. Also provided are methods of detecting cells in a flow cytometer. Other aspects of the present disclosure include methods for determining a level of contamination in a flow cell. Computer-readable media and systems, e.g., for practicing the methods summarized above, are also provided. 123-. (canceled)24. A method for detecting cells in a flow cytometer , comprising:flowing a cellular sample comprising cells through a flow cell of a flow cytometer;detecting optical signals from the cells flowing through the flow cell at a first gain setting; anddetecting optical signals from the cells flowing through the flow cell at the second gain setting, wherein the second gain setting is different from the first gain setting.25. The method according to claim 24 , wherein the second gain setting is greater than the first gain setting.26. The method according to claim 25 , wherein the second gain setting is to account for the cellular sample having a high cell concentration.27. The method according to claim 24 , wherein the second gain setting is less than the first gain setting.28. The method according to claim 27 , wherein the second gain setting is to account for the cellular sample having a low cell concentration.29. The method according to claim 24 , wherein the first and second gain settings comprise a photo diodes gain setting claim 24 , a photo multiplier tubes (PMT) gain setting claim 24 , or both.30. The method according to claim 24 , wherein the cellular sample is a blood sample.31. The method according to claim 24 , comprising:analyzing the optical signals detected at the first gain setting to detect a first cell type; andanalyzing the optical signals detected at the second gain setting to detect a second cell type.32. The method according to claim 31 , wherein the first gain setting is higher than the second gain setting claim 31 , and wherein platelets are detected at the ...

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Номер записи: 75

02-01-2020 дата публикации

Visual protocol designer

Номер: US20200004512A1

Автор: Shervin Javadi

Принадлежит: Stratedigm Inc

Disclosed is a graphical user interface to quickly build a graphical representation defining the set of instructions in a protocol without the user needing the programming knowledge to encapsulate those instructions in executable code. The graphical representation may include an arrangement of one or more graphical elements, with each graphical element corresponding to instructions or program logic. The user may also specify the set of parameters associated with each of the graphical elements. The arrangement of the one or more graphical elements, along with the set of parameters for each of the graphical elements, may be used to translate the graphical representation of the protocol into executable code for the protocol. The executable code for the protocol may then be executed by various flow cytometry machines in order to perform the protocol.

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Номер записи: 76

20-01-2022 дата публикации

METHOD FOR DETECTING AND MONITORING THE FORMATION OF BIOFILMS

Номер: US20220017937A1

Автор: BADEL-BERCHOUX Stéphanie, BLAYSAT Benoît, BOUDAREL Héloïse, GREDIAC Michel, MATHIAS Jean-Denis, PROVOT Christian

Принадлежит:

The present invention relates to a method for detecting and/or tracking and/or characterizing the formation of a biofilm. The present invention also relates to a device for detecting and/or characterizing the formation of a biofilm suitable for implementing the method. The present invention can be used in particular in the analytical fields, in biological and enzymological research, in the pharmaceutical field and/or in the medical field. 1. A method for detecting and/or tracking and/or characterizing the formation of a biofilm comprising the following steps:a) carrying out a temporal succession of observations of a solution comprising at least one microorganism and a plurality of particles while the solution is maintained under conditions allowing the development of a biofilm by said at least one microorganism,b) detecting the presence of the biofilm and/or characterizing the kinetics of biofilm formation on the basis of a comparative statistical analysis of the displacements of the particles observed during the various observations.2. The method according to claim 1 , wherein each observation comprises claim 1 , for each particle of a set of particles observed during said observation claim 1 , a determination of a trajectory corresponding to successive displacements made by said particle during said observation.3. The method according to comprising an overall statistical analysis of the displacements made by the particles observed during each observation and a calculation of characteristic times of the formation of the biofilm on the basis of the results of the overall statistical analysis.4. The method according to claim 3 , wherein the overall statistical analysis includes a calculation for each observation of a value of at least one statistical parameter of a displacement distribution carried out respectively by the particles of the plurality of particles and an analysis of the variations as a function of time of the values of said at least one statistical ...

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Номер записи: 77

20-01-2022 дата публикации

DETECTION METHOD AND DETECTION DEVICE

Номер: US20220018753A1

Автор: ARIMOTO Satoshi

Принадлежит:

A target substance detection method includes forming a complex by causing a target substance and a dielectric particle to bind to each other, the dielectric particle being modified with a substance (for example, an antibody) having a property of specifically binding to the target substance; subjecting a bound particle and an unbound particle to dielectrophoresis in a liquid, the bound particle being the dielectric particle constituting the complex, the unbound particle being a dielectric particle not constituting the complex; and detecting the target substance in the complex, based on a difference in motion between the bound particle and the unbound particle caused by the dielectrophoresis. 1. A detection method comprising:forming a complex by causing a target substance and a dielectric particle to bind to each other, the dielectric particle being modified with a substance having a property of specifically binding to the target substance;subjecting a bound particle and an unbound particle to dielectrophoresis in a liquid, the bound particle being the dielectric particle constituting the complex, the unbound particle being a dielectric particle not constituting the complex; anddetecting the target substance included in the complex, based on a difference in motion between the bound particle and the unbound particle caused by the dielectrophoresis, whereina rotating electric field is produced in the liquid to subject the bound particle and the unbound particle to the dielectrophoresis,the bound particle moves to draw a circular path by the dielectrophoresis, andthe unbound particle rotates around an axis passing through a center position of the unbound particle by the dielectrophoresis.2. The detection method according to claim 1 , whereinthe dielectric particle includes a fluorescent substance, andin the detecting of the target substance, the liquid is irradiated with excitation light and fluorescence emitted by the fluorescent substance included in each of the bound ...

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Номер записи: 78

20-01-2022 дата публикации

METHOD FOR CHARACTERISING A PARTICLE ON THE BASIS OF A HOLOGRAM

Номер: US20220018756A1

Автор: Allier Cédric, BLANDIN Pierre, CIONI Olivier, Dinten Jean-Marc, Herve Lionel, Joly Pierre

Принадлежит:

A method for characterizing a particle present in a sample, the sample lying between an image sensor and a light source and the sensor lying in a detection plane, includes illuminating the sample with the light source which emits an incident light wave propagating along a propagation axis, and acquiring an image of the sample with the sensor. The sensor is exposed to an exposure light wave. The image includes a plurality of elementary diffraction patterns each corresponding to one particle. The method also includes reconstructing a complex image representative of a complex amplitude of the light wave on a reconstruction surface passing through the sample, based on the acquired image; selecting a region of interest of the complex image corresponding to a particle of interest; forming an extracted image based on the region of interest; and characterizing the particle of interest depending on the extracted region of interest. 117-. (canceled)18. A method for characterizing a particle within a sample , the sample lying between an image sensor and a light source , the image sensor lying in a detection plane , the method comprising:a) illuminating the sample with the light source, the light source emitting an incident light wave that propagates along a propagation axis;b) acquiring an image of the sample with the image sensor, the image comprising a plurality of elementary diffraction patterns, each elementary diffraction pattern corresponding to one particle;c) on the basis of the acquired image, reconstructing a complex image representative of a complex amplitude of the exposure light wave on at least one reconstruction surface passing through the sample, the reconstruction being achieved by implementing an iterative reconstruction algorithm, the algorithm comprising, in each iteration, updating a phase of the exposure light wave in the detection plane or on the reconstruction surface;d) selecting a region of interest of the complex image, the selected region of ...

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Номер записи: 79

10-01-2019 дата публикации

SYSTEM AND METHODS FOR MANAGING BLOOD LOSS OF A PATIENT

Номер: US20190008427A1

Автор: MILLER Kevin J, Satish Siddarth, Zandifar Ali

Принадлежит:

One variation of the method for managing blood loss of a patient includes: receiving an image of a physical sample; extracting a feature from an area of the image corresponding to the physical sample; estimating a blood volume indicator of the physical sample according to the extracted feature; estimating a patient blood loss based on the blood volume indicator; estimating a euvolemic patient hematocrit based on an estimated patient blood volume and the estimated patient blood loss; receiving a measured patient hematocrit; and generating a volemic status indicator based on a comparison between the measured patient hematocrit and the estimated euvolemic patient hematocrit. 117-. (canceled)18. A method for managing blood loss of a patient , comprising:tracking a quantity of a fluid administered to the patient intravenously;receiving an image of a physical sample;extracting a feature from an area of the image correlated with the physical sample;estimating a red blood cell content of the physical sample based on the extracted feature;estimating an extracorporeal blood content of the physical sample based on the estimated red blood cell content of the physical sample; andestimating a hematocrit of the patient based on a previous hematocrit of the patient, the quantity of the fluid administered to the patient, and the estimated extracorporeal blood content of the physical sample.19. The method of claim 18 , wherein tracking the quantity of the fluid administered to the patient intravenously comprises tracking the quantity of a known composition administered to the patient according to a transfusion rate claim 18 , wherein estimating hematocrit of the patient comprises estimating the hematocrit of the patient further based on the known composition of the fluid.20. The method of claim 18 , wherein extracting the feature from the area of the image correlated with the physical sample comprises extracting a subset of pixels from a set of pixels within the area of the image.21. ...

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Номер записи: 80

11-01-2018 дата публикации

FLUID PUMPING AND TEMPERATURE REGULATION

Номер: US20180008979A1

Автор: Giri Manish, Sait M A Shameed, Smith Matthew David, YU Joshua M.

Принадлежит: Hewlett-Packard Development Company, L.P.

Fluid may be pumped within a microfluidic channel across a cell/particle sensor using a microscopic resistor. The microscopic resistor may be selectively actuated so as to heat the fluid within the microfluidic channel to a temperature below a nucleation energy of the fluid so as to regulate a temperature of the fluid for at least when the cell/particle sensor is sensing the fluid. 1. An apparatus comprising:a microfluidic channel to receive a fluid;an analyte sensor within the microfluidic channel;a microscopic resistor in the microfluidic channel; and actuate the microscopic resistor to a fluid pumping state in which fluid adjacent the microscopic resistor is heated to a temperature above a nucleation energy of the fluid to pump the fluid across the cell/particle sensor; and', 'selectively actuate the microscopic resistor to a temperature regulating state in which fluid adjacent the microscopic resistor is heated to a temperature below the nucleation energy of the fluid, wherein the controller is to selectively actuate the microscopic resistor to the temperature regulating state to regulate a temperature of the fluid for at least when the analyte sensor is sensing the fluid., 'a controller to2. The apparatus of further comprising a temperature sensor to output temperature signals indicative of a temperature of the fluid claim 2 , wherein the controller is to selectively actuate the microscopic resistor to the temperature regulating state based upon the temperature signals.3. The apparatus of comprising:a cassette containing a microfluidic diagnostic chip, the microfluidic diagnostic chip comprising the microfluidic channel, the temperature sensor and the microscopic resistor; anda portable electronic device containing the controller, wherein the cassette is releasably connectable to the portable electronic device.4. The apparatus of claim 1 , wherein the controller is to selectively actuate the microscopic resistor so as to apply different amounts of heat when in ...

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Номер записи: 81

11-01-2018 дата публикации

DIAGNOSTIC CHIP

Номер: US20180008983A1

Автор: Domingue Chantelle M., Giri Manish, McGuinness Nicholas, Sells Jeremy

Принадлежит: Hewlett-Packard Development Company, L.P.

A microfluidic diagnostic chip may comprise a main fluid channel comprising a main pump, a secondary fluid channel branching off from the main fluid channel, and a secondary pump within the secondary fluid channel wherein the secondary pump is to pull a particle of analyte of a first size from a fluid passing through the main channel, the fluid comprising particles of analyte of the first size and of a number of larger sizes. A method of analyzing an analyte on a microfluidic chip may comprise pumping, with a main microfluidic pump, a fluid comprising an analyte particle through a main microfluidic channel fluidly coupled to a fluid slot and sorting the analyte particle within the fluid through a secondary microfluidic channel by pulling the analyte particle into the secondary microfluidic channel with a secondary microfluidic pump. 1. A microfluidic diagnostic chip , comprising:a main fluid channel comprising a main pump;a secondary fluid channel branching off from the main fluid channel; anda secondary pump within the secondary fluid channel wherein the secondary pump is to pull a particle of analyte of a first size from a fluid passing through the main channel, the fluid comprising particles of analyte of the first size and of a number of larger sizes.2. The microfluidic diagnostic chip of claim 1 , wherein the secondary fluid channel comprises a smaller diameter than the main fluid channel.3. The microfluidic diagnostic chip of claim 2 , wherein the secondary fluid channel comprises a first sensor to count a number of the particles of the first size passing therethrough.4. The microfluidic diagnostic chip of claim 2 , wherein the diameter of the secondary fluid channel excludes the number of larger sizes of particles.5. The microfluidic diagnostic chip of claim 3 , wherein the main fluid channel comprises a second sensor to count particles of the analyte of the first size and larger sizes passing therethrough.6. The microfluidic diagnostic chip of claim 5 , ...

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Номер записи: 82

12-01-2017 дата публикации

Sequencing of nucleic acids via barcoding in discrete entities

Номер: US20170009274A1

Автор: Adam R. Abate, Adam R. SCIAMBI, Freeman Lan, John Halliburton

Принадлежит: UNIVERSITY OF CALIFORNIA

Microfluidic methods for barcoding nucleic acid target molecules to be analyzed, e.g., via nucleic acid sequencing techniques, are provided. Also provided are microfluidic, droplet-based methods of preparing nucleic acid barcodes for use in various barcoding applications. The methods described herein facilitate high-throughput sequencing of nucleic acid target molecules as well as single cell and single virus genomic, transcriptomic, and/or proteomic analysis/profiling. Systems and devices for practicing the subject methods are also provided.

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Номер записи: 83

14-01-2021 дата публикации

Cell capture in microfluidic devices

Номер: US20210008554A1

Автор: Johan ELF, Martin Lovmar, Mikael Olsson, Ove Ohman, Ozden Baltekin

Принадлежит: Astrego Diagnostics AB

A capturing of target cells from a biological sample is achieved by inducing a flow of the biological sample in a flow channel (30, 60) of an upstream microfluidic device (1). Target cells present in the biological sample are captured in cell channels (20) of the upstream microfluidic device(1). Once at least a minimum number of target cells are captured in the cell channels (20), the flow of the biological sample in the flow channel is reduced and are verse flow is applied at the upstream microfluidic device (1) to release the target cells captured in the cell channels (20) of the upstream microfluidic device (1) and enable transfer the target cells into cell channels (120) of a downstream microfluidic device (100).

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Номер записи: 84

27-01-2022 дата публикации

NUCLEIC ACID SEQUENCING SYSTEMS

Номер: US20220025457A1

Автор: CHEN Steve Xiangling, FULLER Derek, GUO Minghao, Previte Michael, Zhou Chunhong

Принадлежит:

Methods and systems for sequencing a nucleic acid molecule are described that comprise imaging a first surface and an axially-displaced second surface using a compensation-free optical system, the system comprising an objective lens and at least one image sensor, wherein said optical system has a numerical aperture (NA) of less than 0.6 and a field-of-view (FOV) of greater than 1.0 mm; and) processing the images of the first surface and the axially-displaced second surface to correct for optical aberration such that the images of the first surface and the axially-displaced second surface have substantially the same optical resolution. 1. A system for sequencing a nucleic acid molecule comprising:a) an optical system comprising an objective lens and at least one image sensor, wherein said optical system has a numerical aperture (NA) of less than 0.6, and is configured to acquire images of a first surface and an axially-displaced second surface; and i) process images of the first surface and the axially-displaced second surface to correct for optical aberration such that the images of the first surface and the axially-displaced second surface have substantially the same optical resolution; and', 'ii) detect a fluorescently-labeled composition comprising the nucleic acid molecule, or a complement thereof, disposed on the first surface or the axially-displaced second surface to determine an identity of a nucleotide in the nucleic acid molecule., 'b) a processor programmed to2. The system of claim 1 , wherein the images of the first surface and the axially-displaced second surface are acquired without moving an optical compensator into an optical path between said objective lens and said at least one image sensor.3. The system of claim 1 , wherein the images of the first surface and the axially-displaced second surface are acquired by just refocusing the optical system.4. The system of claim 1 , wherein the numerical aperture is greater than 0.3.5. The system of claim 1 ...

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Номер записи: 85

09-01-2020 дата публикации

APPARATUS FOR PERFORMING CONTACTLESS OPTICALLY-INDUCED DIELECTROPHORESIS FOR SEPARATION OF CIRCULATING TUMOR CELLS

Номер: US20200009581A1

Автор: Wu Min-Hsien

Принадлежит:

A method for performing contactless ODEP for separation of CTCs is provided with the steps of obtaining patients' blood with rare cell suspected CTCs; adding at least one fluorescent antibody binding to CTCs into the blood; staining the blood; injecting the stained blood with fluorescent dye into an ODEP device and then performing fluorescent image identification; trapping the CTCs with at least one fluorescent antibody in the ODEP device by creating an image pattern and then generating an ODEP force; Separating the trapped CTCs from other non-CTCs cells; absorbing the trapped CTCs; and obtaining a high purity of CTCs. An apparatus for performing contactless ODEP for separation of CTCs is also provided. 15-. (canceled)6. An apparatus for performing contactless optically-induced dielectrophoresis for separation of circulating tumor cells , the apparatus comprising:an ODEP device including a first conductive glass, a bio-compatible membrane, and a second conductive glass wherein the bio-compatible membrane is disposed below the first conductive glass, the bio-compatible membrane includes a transverse main channel and a longitudinal micro channel perpendicular to the main channel and joining the main channel at a cell separation zone; the first conductive glass includes a first hole and a second hole aligned with two ends of the main channel respectively, and a third hole aligned with one end of the micro channel; and the second conductive glass is disposed below the bio-compatible membrane;a sample receiving member disposed on and aligned with the first hole;an exhaust discharge member disposed on and aligned with the second hole;a target collection member disposed on and aligned with the third hole; anda controller including an optical projection device and an image fetch device.7. The apparatus of claim 6 , further comprising a first electrode channel disposed along an edge of the first conductive glass and corresponding to the main channel claim 6 , and a second ...

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Номер записи: 86

27-01-2022 дата публикации

Complex particle measurement apparatus

Номер: US20220026330A1

Автор: Hisashi Akiyama, Makoto Nagura, Takashi KIMBA, Takeshi Akamatsu, Yasuhiro Tatewaki, Yohei Oka

Принадлежит: Horiba Ltd

A complex particle measurement apparatus comprising a first light source that irradiates a first storage cell; a photodetector that detects intensity of light; a second light source that irradiates a second storage cell; an imaging unit that images a particle group; an image data output unit that outputs image data; a supporter that supports the first storage cell and the second storage cell; and a communication pipe that connects the first storage cell and the second storage cell to pass a sample solution, wherein the first storage cell and the second storage cell have bottom surfaces located at positions different from each other, and the communication pipe is laid such that a channel from the first storage cell to the second storage cell has an incline of not less than 0 or not more than 0.

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Номер записи: 87

27-01-2022 дата публикации

METHOD FOR DETECTING PARTICLES OR AEROSOL IN A FLOWING FLUID, COMPUTER PROGRAM, AS WELL AS ELECTRICAL MEMORY MEDIUM

Номер: US20220026338A1

Автор: Rusanov Radoslav

Принадлежит:

A method for detecting particles or aerosol in a flowing fluid, using the principle of laser-induced incandescence. The method includes the following steps: a. focusing a laser light originating from a laser in a spot; b. conducting a fluid which includes particles or aerosol through the spot; c. detecting a thermal radiation originating from the spot with the aid of a detector; and d. evaluating a variable which is provided by the detector and characterizes the detected thermal radiation within time intervals, the duration of the time intervals being dependent on a velocity of the fluid. 114-. (canceled)15. A method for detecting particles or aerosol in a flowing fluid , using laser-induced incandescence , the method comprising the following steps:a. focusing a laser light originating from a laser in a spot;b. conducting the fluid which includes particles or aerosol through the spot;c. detecting a thermal radiation originating from the spot using a detector; andd. evaluating a variable which is provided by the detector and characterizes the detected thermal radiation within time intervals, a duration of the time intervals being dependent on a velocity of the fluid.16. The method as recited in claim 15 , wherein at least several of the time intervals overlap.17. The method as recited in claim 16 , wherein the duration of the time intervals is greater than an expected full width at half maximum (FWHM) of the variable characterizing the thermal radiation.18. The method as recited in claim 17 , wherein the duration of the time intervals is 1 to 2 times the expected FWHM.19. The method as recited in claim 18 , wherein the duration of the time intervals is 1.5 times the expected FWHM.20. The method as recited in claim 16 , wherein an overlapping time period of the time intervals corresponds to at least half the duration of the time interval.21. The method as recited in claim 16 , wherein a particle is considered to be detected when the variable characterizing the thermal ...

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Номер записи: 88

27-01-2022 дата публикации

METHODS AND DEVICES FOR EVALUATING PERFORMANCE OF A DIODE LASER

Номер: US20220026339A1

Автор: Amiri Waheed, Gianduzzo David J., Nguyen An-Dien

Принадлежит:

Methods for evaluating performance a diode laser are provided. In embodiments, methods include receiving a laser beam profile of a diode laser, determining first, second and third laser beam widths at first, second and third laser intensities, respectively, for the laser beam profile, computing a first ratio between the second and third laser beam widths, computing a second ratio between the first and second laser beam widths, evaluating laser performance based on the first and second ratios, and outputting a determination regarding the suitability of the laser for use in a flow cytometry setting. Devices for practicing the subject methods are also provided, and include first and second stages configured to receive a diode laser and beam profiler, respectively. Aspects of the invention further include flow cytometers incorporating a diode laser that has been evaluated by the subject method. 1. A method for evaluating performance of a diode laser , the method comprising:receiving a laser beam profile of the diode laser, the laser beam profile comprising laser beam width data and laser intensity data;determining first, second and third laser beam widths at first, second and third laser intensities, respectively, for the laser beam profile;computing:a first ratio between the second and third laser beam widths; anda second ratio between the first and second laser beam widths; andevaluating laser performance based on the first and second ratios.2. The method according to claim 1 , wherein the diode laser is a semiconductor laser diode.35-. (canceled)6. The method according to claim 1 , wherein evaluating performance of the diode laser comprises assessing the extent to which the laser beam profile deviates from a Gaussian beam shape.7. The method according to claim 6 , wherein assessing the extent to which the laser beam profile deviates from a Gaussian beam shape comprises identifying whether multiple modes are present in the laser beam profile.8. The method according to ...

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Номер записи: 89

27-01-2022 дата публикации

MICROFLUIDIC SYSTEM AND METHOD WITH FOCUSED ENERGY APPARATUS

Номер: US20220026341A1

Автор: Appleyard David, Betthauser Jeff, Faust Marjorie, Larsen John, Shao Guocheng, Xia Zheng, Zhou Yu

Принадлежит:

A method of inseminating an animal including flowing a stream of a population of sperm cells through a channel, differentiating the sperm cells into two subpopulations of X-chromosome containing sperm cells and Y-chromosome containing sperm cells, selecting a desired subpopulation, ablating an undesired subpopulation, and collecting both the subpopulations of sperm cells including the desired subpopulation and the ablated undesired subpopulation together, wherein the collected population of sperm cells is used to fertilize an egg. 1. A method for producing a sexed composition of sperm comprising non-human animal sperm by identifying a subpopulation of sperm from a population of sperm in a sample fluid , the method comprising:staining the population of sperm in the sample fluid with a dye;defining a gating strategy for differentiating the subpopulation of sperm in the sample fluid in the population of sperm based on one or more physical characteristics of sperm in the population of sperm, wherein the one or more physical characteristics are identifiable within the population of sperm based in part on the dye staining, and wherein the gating strategy comprises identifying one or more desired physical characteristics of the subpopulation of sperm;measuring the one or more physical characteristics for the sperm in the population of sperm;identifying sperm in the subpopulation of sperm based on the measured one or more physical characteristics and the defined gating strategy; andgenerating the sexed composition of sperm comprising the subpopulation of sperm identified based on the measured one or more physical characteristics and the defined gating strategy.2. The method of claim 1 , wherein the population of sperm in the sample fluid comprises bovine sperm.3. The method of claim 1 , wherein the sexed composition of sperm substantially comprises X chromosome-bearing sperm cells.4. The method of claim 1 , wherein the sexed composition of sperm substantially comprises Y ...

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Номер записи: 90

12-01-2017 дата публикации

METHOD AND DEVICE FOR DETERMINING CHARACTERISTIC PROPERTIES OF A TRANSPARENT PARTICLE

Номер: US20170010197A1

Автор: SCHAEFER Walter, TROPEA Cameron

Принадлежит:

The invention relates to a method for determining the size d of a transparent particle, according to which method the particle is illuminated with light from a light source, a radiation detector measures a time-resolved intensity profile of light of the light source scattered by the particle, a reflection peak () and a refraction peak are determined in the intensity profile and the size d of the particle is determined based on a time difference between the reflection peak () and the refraction peak. The method according to the invention is characterized in that the time-resolved intensity profile is measured at a definable scattering angle θs, a first second-order refraction peak () and a second second-order refraction peak () having a mode different from that of the first refraction peak () being determined, a characteristic variable γ being determined as the ratio of a first time difference Δtbetween the reflection peak () and the first refraction peak () and of a second time difference Δtbetween the reflection peak () and the second refraction peak (), and the size of only those particles being determined for which the characteristic variable γ corresponds to a definable value. 1. A method for determining characteristic properties of a transparent particle , wherein the particle is illuminated with light from a light source , wherein a time-resolved intensity profile of light from the light source that is scattered at the particle is measured by a radiation detector at a predefinable scattering angle θ , wherein characteristic scattered light peaks are determined in the intensity profile , and wherein a size of the particle is determined based on a time difference between two scattered light peaks ,wherein a first time difference is determined between a first pair of scattered light peaks and a second time difference is determined between a second pair of scattered light peaks, a characteristic variable is determined from the ratio of the first time difference ...

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Номер записи: 91

14-01-2016 дата публикации

Methods and Apparatus for Real-Time Detection and Clearing of a Clog

Номер: US20160011094A1

Автор: Aaron B. Kennington

Принадлежит: Intellicyt Corp

A flow cytometer apparatus and methods for detecting and clearing a clog therein are disclosed. An example method for detecting a clog may include (i) detecting, via a fault detection system of a flow cytometer, a first plurality of events associated with a first aliquot from a first sample well, (ii) determining a count of the first plurality of events associated with the first aliquot, (iii) determining whether the count of the first plurality of events is below a minimum count tolerance and (iv) (a) if the count of the first plurality of events is below the minimum count tolerance, then determining that the flow cytometer has a clog, (b) if the count of the first plurality of events is equal to or above the minimum count tolerance, then detecting a second plurality of events associated with a second aliquot from a second sample well.

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Номер записи: 92

14-01-2016 дата публикации

Flow Cytometry Apparatus and Methods

Номер: US20160011096A1

Автор: Vacca Giacomo

Принадлежит:

A particle analyzer, comprising a source of a substantially nondiffracting light beam; a flow path configured to produce in a flowcell a ribbon-like core stream having a cross-sectional aspect ratio of at least 4 and a largest cross-sectional dimension of at least 50 micrometers; the flowcell being configured to expose a segment of the core stream to the light beam; a detector configured to receive a signal resulting from an interaction of a particle in the core stream with the light beam; a first sorting actuator connected with the flowcell, downstream of the exposed segment of core stream; a plurality of sorting channels in fluid connection with the flow path and downstream of the first actuator; the actuator having multiple actuation states, each state configured to direct at least one part of the core stream to a corresponding channel; a second sorting actuator connected with the flowcell, opposite the first actuator, and operable in coordination with the first actuator. 1. A particle analyzer , comprising:a source of a non-Gaussian, substantially nondiffracting light beam;a flow path configured to produce a ribbon-like core stream in a flowcell, said core stream having a cross-sectional aspect ratio of at least 4 and a largest cross-sectional dimension of at least 50 micrometers;said flowcell being configured to expose a segment of said core stream to said light beam; anda detector configured to detect a signal from said core stream, the signal resulting from an interaction of a particle in said core stream with said light beam.2. The particle analyzer of claim 1 , further comprising:a first sorting actuator connected with said flowcell and downstream of said segment of said core stream exposed to said light beam; anda plurality of sorting channels in fluid connection with said flow path and downstream of said first sorting actuator;said first sorting actuator having multiple actuation states, each actuation state configured to direct at least one part of said ...

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Номер записи: 93

14-01-2016 дата публикации

COMPOUND OPTICAL FLOW CELLS AND METHOD OF MANUFACTURE AND USE

Номер: US20160011098A1

Автор: ARBOLEDA Carlos Alberto, CANO Jose M., CLARKIN James P., GRAHAM Marshall Donnie, GRAHAM William Gerry, SANCHEZ Armando J., WELLS Mark A.

Принадлежит:

An improved optical flow cell adapted for use in a flow cytometer for differentiating formed bodies (e.g., blood cells) in liquid suspensions. Preferably manufactured by assembling, aligning, and optically joining at least two elements made from transparent material, the improved flow cell has a seamless internal flow channel of preferably non-circular cross-section in a cylindrical first element through which prepared samples can be metered and an independent second element having an external envelope suited to acquisition of optical parameters from formed bodies in such suspensions, the second element being conforming and alignable to the first element so that non-axisymmetric refractive effects on optical characterizing parameters of formed bodies passing through the flow channel in the first element may be minimized before the two elements are optically joined and fixed in working spatial relationship. 1. A method for making a transparent compound optical flow cell of the type used to characterize formed bodies passing through the flow cell , the optical flow cell having formed therein a rectilinear internal flow channel , the method comprising the steps of:providing a cylindrical monolithic preform comprising a thick-wall glass tube having an axially-extending channel therethrough and a transition temperature, the channel comprising a substantially uniform original cross-section of a desired shape;heating the preform to a predetermined temperature above the transition temperature of the glass tube;axially drawing the preform at a controlled rate, for a controlled time, and at a constant angular orientation, to achieve a desired reduced cross-sectional area of the axially-extending channel;providing an optical element, the optical element comprising a conforming surface that conforms to a segment of the drawn preform, and an exterior non-cylindrical envelope of predetermined form and orientation relative to the conforming surface;assembling the optical element ...

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Номер записи: 94

14-01-2016 дата публикации

Method and device for examining myocardial toxicity and evaluating cardiomyocytes

Номер: US20160011176A1

Автор: Fumimasa Nomura, Kenji Yasuda, Tomoyuki Kaneko

Принадлежит: Tokyo Medical and Dental University NUC

Regarding cardiomyocytes and fibroblasts, it is meaningful to develop a device or system whereby, upon the transmission of pulsation from an adjacent cardiomyocyte or fibroblast, cell potential and cell morphology can be accurately measured on a single cell basis and the toxicity of a drug on cardiomyocytes can be examined on the basis of accurately measured cell potential and cell morphology of a single cell. In the present invention, a mass of cardiomyocytes is disposed on a transparent substrate and the qualities of the cardiomyocytes are evaluated depending on the response of the cardiomyocytes to a forced pulsation stimulus that is applied to the pulsating cardiomyocytes. A mass of cardiomyocytes, said mass being disposed on the transparent substrate, is exposed to a flow of a drug-containing liquid so as to allow the drug to act on cells configuring a network. The level of myocardial toxicity of the drug is evaluated by measuring the fluctuations that are obtained by comparing adjacent pulsating cardiomyocytes in the network.

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Номер записи: 95

14-01-2016 дата публикации

METHODS FOR HIGH THROUGHPUT RECEPTOR:LIGAND IDENTIFICATION

Номер: US20160011204A1

Автор: Almo Steven C., Garrett-Thomson Sarah C., Hillerich Brandan S., III Ronald D., Love James D., Seidel

Принадлежит:

Methods and systems for high-throughput identification of receptor:ligand interactions are provided. 1106.-. (canceled)107. A system comprising:a) a first plurality of suspension-adapted cells, each of which first plurality of cells expresses a first heterologous polypeptide fused to a first fluorescent polypeptide; and i) a second plurality of suspension-adapted cells, each of which second plurality of cells expresses a second heterologous polypeptide fused to a second fluorescent polypeptide, wherein the second heterologous polypeptide is a ligand or a co-receptor for the first heterologous polypeptide, and wherein the second fluorescent polypeptide is distinguishable from the first fluorescent polypeptide;', 'ii) a microbead comprising a second polypeptide that is a ligand or a co-receptor for the first heterologous polypeptide linked to the microbead via a polypeptide linker, wherein the polypeptide linker comprises a detectable label moiety; or', 'iii) an immunoglobulin (Ig) Fc fused to a second polypeptide that is a ligand or a co-receptor for the first heterologous polypeptide, wherein the Ig Fc comprises a detectable label moiety., 'b) a challenge component, wherein the challenge component comprises108. The system of claim 107 , wherein the first plurality of cells is immobilized on a surface.109. The system of claim 108 , wherein the first plurality of cells is immobilized on a surface in a spatially restricted manner.110. The system of claim 107 , wherein the first plurality of cells is suspended in a liquid medium.111. The system of claim 107 , wherein the first heterologous polypeptide is a polypeptide that mediates cell-cell interactions claim 107 , an immunoglobulin superfamily polypeptide claim 107 , a TNF superfamily polypeptide claim 107 , a TNR receptor superfamily polypeptide claim 107 , a G-protein coupled receptor claim 107 , a growth factor receptor claim 107 , a nectin claim 107 , an interleukin receptor claim 107 , an ion channel claim 107 , ...

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Номер записи: 96

11-01-2018 дата публикации

OPTICAL PARTICLE SORTER

Номер: US20180010997A1

Автор: CURRY JOHN J., LEVINE ZACHARY H.

Принадлежит:

A process for optically sorting a plurality of particles includes: providing a particle receiver; producing particles; receiving the particles by the particle receiver; receiving a light by the particle receiver; producing a standing wave optical interference pattern in an optical interference site of the particle receiver from the light; subjecting the particles to an optical gradient force from the standing wave optical interference pattern; deflecting the particles into a plurality of deflected paths to form the sorted particles from the particles; and propagating the sorted particles from the optical interference site through the deflected paths to optically sort the particles 1. An optical particle sorter comprising: a particle entrance that receives a plurality of particles;', 'an optical entrance that receives light and that is geometrically disposed at a non-parallel angle with respect to the particle entrance;', 'a sorted particle exit opposing the particle entrance and that communicates sorted particles from an optical interference site; and', 'the optical interference site interposed between the particle entrance and the sorted particle exit;, 'a particle receiver comprising produces a first light; and', 'produces a standing wave optical interference pattern in the optical interference site of the particle receiver; and, 'a first light source in optical communication with the particle receiver and that provides the particles; and', {'b': '24', 'communicates the particles to the particle receiver at an acute angle with respect to the standing wave optical interference pattern ,'}], 'a particle source in fluid communication with the particle receiver and thatwherein the optical particle sorter sorts the particles into a plurality of sorted particles that exit the particle receiver at the sorted particle exit, andthe sorted particles propagate in a plurality of deflected paths relative to a path of propagation of the particles at the particle entrance, the ...

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Номер записи: 97

11-01-2018 дата публикации

EVALUATING BIOLOGICAL MATERIAL FOR UNASSOCIATED VIRUS-SIZE PARTICLES WITH ADENOVIRUS OR ADENO-ASSOCIATED VIRUS EPITOPE

Номер: US20180010998A1

Автор: Artinger Michael A., Gates Tyler Donald, Kohlmeier Francis Kevin, Olszowy Michael W.

Принадлежит:

A method for evaluating a biological material for unassociated virus-size particles having a particular epitope indicative of an adeno-associated virus viral type or an adenovirus viral type uses a fluorescent antibody stain specific for binding with the epitope and a fluid sample with the virus-size particles and fluorescent antibody stain is subjected to flow cytometry with identification of fluorescent emission detection events indicative of passage through a flow cell of a flow cytometer of unassociated labeled particles of virus size including such a virus-size particle and fluorescent antibody stain. 1. A flow cytometry method for evaluating a biological material sample for unassociated virus-size particles of a viral type selected from the group consisting of an adenovirus viral type and an adeno-associated virus viral type , the method comprising: flowing the fluid sample through a flow cell of a flow cytometer;', 'subjecting the fluid sample flowing through the flow cell to excitation radiation capable of causing a fluorescent emission response from the fluorescent antibody stain; and', 'detecting radiation from the flow cell within a wavelength range of the fluorescent emission and evaluating the detected radiation to identify detection events indicative of passage through the flow cell of the unassociated labeled particles of virus size including a said virus-size particle of the viral type bound with a portion of the fluorescent antibody stain;, 'subjecting to flow cytometry a fluid sample comprising at least a portion of the biological material sample, wherein the fluid sample comprises a fluorescent antibody stain capable of binding, directly or indirectly, with an epitope of the viral type, the flow cytometry comprisingwherein the unassociated labeled particles of virus size are of a particle size in a range of from 10 nanometers to 200 nanometer; andwherein the fluid sample as fed to the flow cytometer comprises a concentration of the fluorescent ...

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Номер записи: 98

11-01-2018 дата публикации

METHODS FOR QUANTITATIVE ASSESSMENT OF MUSCLE FIBERS IN MUSCULAR DYSTROPHY

Номер: US20180011000A1

Автор: Krueger Joseph, LANGE Holger, Martin Nathan T., Milici Anthony J., Young George David

Принадлежит: Flagship Biosciences, Inc.

The disclosure concerns a method for assessing muscular dystrophy-linked protein expression in muscle fibers using digital image analysis of tissue. The method relates to assessing disease severity in individuals with muscular dystrophy. Muscle tissue samples are obtained from patients submitted for evaluation and processed to produce tissue sections mounted on glass slides which have been stained for a muscular dystrophy-linked protein. Digital images of the stained tissue sections are generated and analyzed by applying an algorithm process implemented by a computer to the images. The algorithm process extracts the morphometric and staining features of the muscular dystrophy-linked protein staining in the tissue, and parameters relating to these features are used to score the disease status for each patient submitted for evaluation. The score of disease status is ultimately used to infer disease severity, monitor the efficacy of a therapeutic approach, or select patients as candidates for a therapeutic approach. 1. A method comprising:capturing at least one digital image of at least one stained muscle tissue section;extracting at least one image analysis feature from each muscle fiber in the at least one digital image, wherein the at least one image analysis feature is selected from the group consisting of staining features and morphometric features;combining at least one staining and morphometric feature to derive a score of disease status; andinterpreting the score of disease status to draw inferences associated with the severity of disease.2. The method of claim 1 , wherein the at least one tissue section is stained for at least one muscular marker selected from the group consisting of a muscular dystrophy-linked protein and a muscle fiber membrane biomarker.3. The method of claim 2 , wherein the muscular dystrophy-linked protein is a protein product of a gene that when mutated claim 2 , or otherwise disrupted claim 2 , gives rise to at least one muscular ...

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Номер записи: 99

11-01-2018 дата публикации

Automated real-time particle characterization and three-dimensional velocimetry with holographic video microscopy

Номер: US20180011001A1

Автор: David G. Grier, Fook Chiong CHEONG, Ke Xiao

Принадлежит: New York University NYU

An in-line holographic microscope can be used to analyze on a frame-by-frame basis a video stream to track individual colloidal particles' three-dimensional motions. The system and method can provide real time nanometer resolution, and simultaneously measure particle sizes and refractive indexes. Through a combination of applying a combination of Lorenz-Mie analysis with selected hardware and software methods, this analysis can be carried out in near real time. An efficient particle identification methodology automates initial position estimation with sufficient accuracy to enable unattended holographic tracking and characterization.

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Номер записи: 100