High performance liquid chromatography method and apparatus

MODIFIED MEMBRANE SUPPRESSOR AND METHOD OF USE

Suppressor for suppressing the electrolyte of an eluent from a chromatographic column. The suppressor includes a regenerant compartment separated from an effluent compartment by an ion exchange membrane sheet permeable to ions of the same charge as its exchangable ions. Structure including ion exchange sites is provided in one or both compartments extending between the membrane sheet and compartment walls to define a continuous convoluted liquid flow through passage. Suitable structures are a screen or projections from the compartment walls. In a sandwich suppressor, two membrane sheets are included with an effluent flow channel between them. A detector is provided downstream of the suppressor.

PROCESS FOR ANALYTICAL DETERMINATIONS AND ROTOR INSERT ELEMENT THEREFOR

ABSTRACT The present invention provides a process for carrying out analytical determinations by mixing and incubating a sample solution with at least one reagent solution and optical measurement of one parameter in the incubated reaction solution mixture, mixing, incubating and measuring being carried out during the action of a centrifugal force, wherein, under the influence of the centrifugal force, the sample solution is brought together with a soluble dry reagent, with at least partial dissolving thereof, and the mixture or the solution is forced centrifugally through a plurality of small hollow spaces, the centrifugal force and the flow resistance of the small hollow spaces being so adapted with regard to one another that a complete dissolving and mixing of the reaction components and possibly incubation takes place before the reaction solution passes from the small hollow spaces into a measuring chamber in which the measure-ment is carried out. The present invention also provides an insert element for a centrifugal analysis rotor, wherein it comprises at least one analysis reagent in dry form and has a plurality of very small interconnecting hollow spaces which connect a sample providing chamber with a measuring chamber.

PROCESS FOR ANALYTICAL DETERMINATIONS AND ROTOR INSERT ELEMENT THEREFOR

SEQUENTIAL DETECTION ION CHROMATOGRAPHY

Packings or equivalent structures which produce radial mixing in channels can be used to enhance reagent addition devices for ion chromatography post column reactions in which band spreading at detrimental levels is effectively controlled. Application to two dimensional conductometric detection in simultaneous suppressed and nonsuppressed ion chromatography.

Verfahren zur Herstellung von Kapillarsäulen für die Gaschromatographie

Verfahren und Vorrichtung zur Anzeige bzw. Messung mindestens eines oxydierbaren bzw. reduzierbaren Bestandteils eines Eluats auf elektrochemischem Weg

Chromatograph and extraction system

In a liquid/liquid chromatography or extraction p4rocess the mobile phase (2) is injected in the column with the stationary phase (1) at such a speed (40/80 cm/sec.) that at the entry (3) a continuous stream (13) is formed which disintegrates into droplets (14). This permits larger tubes (5) to be used. Their inner diameter B should have a ratio to the length C of 1:30 to 1:70. The entry diameter D should be less than B/2.

DETECTION METHOD AND APPARATUS FOR CHROMATOGRAPHY

Low-retention pre-columns: a straightforward approach to enable larger injection volumes and reduce extra-column effects in HPLC

Low-retention pre-columns that allow increased injection volumes of solvents chromatographically stronger than the mobile phase and use of solvents with limited solubility in the mobile phase, such as ethyl acetate and MTBE. The system and method also reduces band broadening due to the extra-column effects acting upstream of the analytical column, including band broadening due to the injection process and due to the connecting tubing and fittings between the injection system and the column. A pre-column may also be used as a guard column, thereby minimizing band broadening due to the guard column.

SAMPLE ADJUSTING DEVICE AND SAMPLE ADJUSTING DEVICE FORMING METHOD

PROBLEM TO BE SOLVED: To provide a sample adjusting device and a method for removing impurities and undesired substance from a sample, ahead of mass spectrometry by matrix auxiliary laser desorption/ionization (MALDI), electro-spray ionization(ESI) or the like. SOLUTION: A substrate used in this sample adjusting device can be formed in a probe 50 shape or as a standard pipette chip. A chromatography medium is attached to the inner surface of the chip in order to form a single layer of particles easily contactable to a fluid flowing the pipette chip. The device can be used for washing, protein coupling, elution treatment or the like. COPYRIGHT: (C)2001,JPO ...

Verfahren zur selektiven Abtrennung nach einem chromatographischen Verfahren

VERFAHREN ZUR DURCHFUEHRUNG ANALYTISCHER BESTIMMUNGEN UND HIERFUER GEEIGNETES ROTOREINSATZELEMENT

METHOD AND APPARATUS FOR FRACTIONAL SEPARATION OF MIXTURE

1334302 Chromatography MOBIL OIL CORP 22 April 1971 [18 May 1970] 10789/71 Heading B1X The flow of liquid or gaseous fluid through a packed chromatography column 10 is improved by the provision of several axially arranged, helically twisted deflector plates 17. Adjacent plates have opposite twist and their ends are preferably arranged mutually at right angles. Fluid passing helically along the plates is divided at each point of reversal of twist and recombined in such a manner that part of one stream is combined with part of another at the next reversal of twist. In operation specified hydrocarbon mixtures are passed via heated block 14 and line 16 to the heated column 10. Effluent is detected by a thermal conductivity bridge 19 which activates controls to divert portions of effluent to appropriate collectors. The column is packed with specified zeolites, gels and clays and can be pretreated with steam or by caldining. The column may be of the moving bed type.

HIGH SPEEDLIQUID CHROMATOGRAPHY-PROCEED N AND DEVICE

MODIFIED MEMBRANE CHROMATOGRAPHIC ELUENT SUPPRESSOR

Process and measuring device of at least a variable of constitution and concentration of a chromatographic éluat

SELECTIVE SEPARATION BY A CHROMATOGRAPHIC PROCESS

... 1334233 Separating xylenes MOBIL OIL CORP 4 Dec 1970 [5 Dec 1969] 57727/70 Heading B1L A zeolite 0.9 Π0.2 M 2# O:W 2 O 3 :5-100YO 2 : zH 2 O where m is a cation of valency n, W is aluminium or gallium, Y is silicon or germanium and z is 0-40 is used to separate paraxylene from its isomers. Examples are given of the preparation of zeolites ZSM-5 and ZSM-8 useful in the process.

PROCEDURE FOR THE EXECUTION OF ANALYTIC REGULATIONS AND FOR THIS APTITUDE ROTOR EMPLOYMENT ELEMENT.

Sequential detection ion chromatography

SEPARATING COLUMN FOR GAS CHROMATOGRAPHY

MODIFIED MEMBRANE SUPPRESSOR AND METHOD OF USE

HIGH PERFORMANCE LIQUID CHROMATOGRAPHY METHOD AND APPARATUS

High pressure liquid chromatographic apparatus in which a fluid mixture containing at least one solute that is reactive with chromatographically reactive surfaces in the column is loaded into the column, and a number of plugs of different eluant fluids are injected into that column. The injections are made in a manner that minimizes the amount of eluant required. In one embodiment, the injections are made to insure that flow of at least the eluant fluids through the column will occur, preferably with a substantially flat wave front, at speeds corresponding to reduced velocities greater than about 5,000, i.e. at flow rates sufficient to induce turbulent flow in those fluids, thereby minimizing the time required for the entire succession of mixture and eluant fluids to traverse the column. In other embodiments, the injections are made substantially simultaneously at spatially separated points adjacent the entrance to the column or are made in sequence to a single location adjacent the entrance ...

Selective sepn chromatographic process

MODULAR MULTIPLE-COLUMN CHROMATOGRAPHY CARTRIDGE

A cartridge for chromatography separations comprises a housing; at least a first and a second chromatography column at least partially passing through the housing, each chromatography column comprising a first and a second end fitting operable to connect the chromatography to external tubing of a chromatography system.

Capillary chromatographic column

Metal capillary columns for gas chromatography having solid material embedded in their inside surfaces may be made by drawing a larger tube and introducing solid material into the larger tube as it enters the zone of constriction. The solid material is a partitioning agent, e.g. molecular sieve or an inert material, either of which can be subsequently coated with liquid partitioning agent.

Detection method and apparatus for chromatography

... 1, 120, 364. Analyzing fluids electro-chemically. V. PRETORIUS and H. H. HAHN. Oct. 3, 1966 [Oct. 6, 1965; Aug. 2, 1966], No. 44025/66. Heading G1N. The eluate from a chromatographic separation process passes through an electrolytic detection cell in which two electrodes are maintained at such a potential difference as to cause an electrolytic oxidation/reducion of the constituents being monitored and the applied voltage and the electric current flowing through the cell are measured. The separation process may be carried out in a chromatographic column or by paper chromatography or starch or gel chromatography (electrophoresis). The eluting agent may be a liquid or a gas. If it is a gas, a film of water may be caused to run at a constant rate over the electrodes or the gas may be bubbled through a liquid in the cell or the gas may flow co-currently with a constant stream of liquid through the detector cell. The liquid may include a solubilising agent for the constituents of the gas. As ...

A method of introducing Samples into Chromatographic separating Systems and Apparatus for Liquid Chromatography

... 1,194,375. Liquid chromatography. V. PRETORIUS and H. H. HAHN, 21 July, 1967 [2 Aug., 1966], No. 33749/67. Heading B1X. A method of electrically introducing a sample of substances amenable to reversible electrodeposition into a chromatographic system for chromatographic separation of the sample comprises (a) bringing a solution of said sample into intimate contact with an electrode, (b) electrolytically depositing said sample from the solution on said electrode, (c) bringing the electrode into intimate contact with at least one of the separating phases of the chromatographic system in an inlet zone of the system and (d) electrolytically redissolving the sample off the electrode in at least said one separating phase.

HIGH SPEED LIQUID CHROMATOGRAPHY PROCEDURE AND DEVICE

SEPARATING COLUMN FOR GAS CHROMATOGRAPHY

METHOD AND APPARATUS FOR FRACTIONAL SEPARATION OF MIXTURE

A TURBULENT FLOW MIXING DEVICE FOR USE IN A CHROMATOGRAPHY SYSTEM

Modified membrane suppressor and method of use

Suppressor for suppressing the electrolyte of an eluent from a chromatographic column. The suppressor includes a regenerant compartment separated from an effluent compartment by an ion exchange membrane sheet permeable to ions of the same charge as its exchangeable ions. Structure including ion exchange sites is provided in one or both compartments extending between the membrane sheet and compartment walls to define a continuous convoluted liquid flow through passage. Suitable structures are a screen or projections from the compartment walls. In a sandwich suppressor, two membrane sheets are included with an effluent flow channel between them. A detector is provided downstream of the suppressor.

Verfahren zur Herstellung von Kapillarsaeulen fuer die Gaschromatographie

Packed separating columns of small diameter for liquid chromatography

... 1,103,943. Liquid chromatography column. BECKMAN INSTRUMENTS Inc. 15 March, 1965 [19 March, 1964], No. 10805/65. Addition to 1,023,496. Heading B1X. A chromatographic column for liquid chromatography comprises tube 22 having inner diameter greater than 1 mm. but not greater than 2 mm and containing a granular partitioning material 24, the individual grains of partitioning material having a dimension greater than 1/5 of the inner diameter of the tube. The granular material may itself be the sorptive material or it may support a layer of partitioning liquid. There is reference to the use of aluminium oxide. The column may be made of glass, copper, stainless steel, aluminium or plastics material and may be made by drawing down a tube of larger diameter filled with the granular material. To obtain a loose packing of the particles of granular material a rod or wire may be inserted into the tube before it is filled, then removed to leave free space between the particles.

Control of sample separation based on analysis of mobile phase supply from mobile phase container

A method of controlling a sample separation apparatus (10, fig 1) for separating a fluidic sample using a mobile phase (I, II, fig 1) provided from at least one mobile phase container (100, fig 1) is dislcosed. The method comprises determining a weight and volume reduction behaviour 132, according to which weight and volume of mobile phase in the mobile phase container are reduced during operation of the sample separation apparatus. The method also comprises determining a tare weight 138 of the mobile phase container based on a gross weight information, which is indicative of an initial gross weight 128 of the mobile phase container; and a volume information, which is indicative of an initial mobile phase volume 130 in the mobile phase container. Also disclosed is a method of controlling a sample separation apparatus involving measuring actual measurement information indicative of conveyance of the mobile phase (such as the weight over time of the mobile phase container), determining operation ...

High performance liquid chromatography method and apparatus

High performance liquid chromatography method and apparatus

HIGH PERFORMANCE LIQUID CHROMATOGRAPHY METHOD AND APPARATUS

High pressure liquid chromatographic apparatus in which a fluid mixture containing at least one solute that is reactive with chromatographically reactive surfaces in the column is loaded into the colum n, and a number of plugs of different eluant fluids are injected into that column. The injections are made in a manner that minimize s the amount of eluant required. In one embodiment, the injections are made to insure that flow of at least the eluant fluids throug h the column will occur, preferably with a substantially flat wave front, at speeds corresponding to reduced velocities greater than about 5 000, i.e. at flow rates sufficient to induce turbulent flow in those fluids, thereby minimizing the time required for the entire succession of mixture and eluant fluids to traverse the column. In other embodiments, the injections are made substantially simultaneously at spatial ly separated points adjacent the entrance to the column or are made in sequence to a single location adjacent the entrance to the column, i n either case the fluids then tend to travel through the column at a common group velocity as closely bunched fluid plugs.

Process for the manufacture of capillary tubes for the chromatography of gases and capillary tubes in conformity with those thus obtained

High performance liquid chromatography method and apparatus

A method of and apparatus for liquid chromatography at improved analytical and preparative speeds and quantities involves flow of fluids through the column at flow rates sufficient to induce turbulent flow in those fluids. In one embodiment, the apparatus and method includes a substantially uniform chromatography column created by packing together a multiplicity of rigid, solid, porous particles having diameters of not less than about 30 mu m, surfaces of the particles being chromatographically active. A fluid mixture containing at least one solute that is reactive with the particle surfaces is injected into the column and subsequently eluted therefrom by a eluant fluid flow, both the injection and elution being effected at a velocity sufficient to induce flow within at least a substantial portion of the interstitial volume between the particles at a reduced velocity greater than about 5,000.

Improvement in chemical analyses

METHODS AND DEVICES FOR MICROFLUIDIC EXTRACTION

HIGH PERFORMANCE LIQUID CHROMATOGRAPHY METHOD AND APPARATUS

A method of and apparatus for liquid chromatography at improved analytical a nd preparative speeds and quantities involves flow of fluids through the column at flow rates sufficient to induce turbulent flow in those fluids. In one embodiment, the apparatus and method includes a substantially uniform chromatography column created by packing together a multiplicity of rigid, solid, porous particles having diameters of not less than about 30 .mu.m, surfaces of the particles being chromatographically active. A fluid mixture containing at least one solute that is reactive with the particle surfaces i s injected into the column and subsequently eluted therefrom by an eluant flui d flow, both the injection and elution being effected at a velocity sufficient to induce flow within at least a substantial portion of the interstitial volume between the particles at a reduced velocity greater than about 5,000.

SELECTIVE SEPARATION BY A CHROMATOGRAPHIC PROCESS

Improvements relating to detection in liquid chromatography

... 1,125,358. Electro-chemical analysis. V. PRETORIUS and H.H. HAHN. July 26, 1967 [Aug. 2, 1966], No.34443/67. Addition to 1,120.364. Heading G1N. The progress of a chromatogram in a liquid chromatography system is monitored by passing a moving liquid phase of the system in contact with a solid detection electrode and observing a constitution and concentration dependent electrical quantity detectable at the detection electrode with reference to a reference electrode connected to the system. The electrical quantity may be changes in the electrical double layer on the detection electrode produced by the zeta effect, or the capacitance between the electrodes, or the conductivity of the eluate. A solute may be detected indirectly by observing the rate of an electrolytic reaction involving oxygen dissolved in the forwarding phase. For measuring conductivity at the outlet of a chromatographic column, the column wall (2), made of stainless steel, may serve as one electrode, and a smaller diameter ...

ANALYTICAL METHOD USING CENTRIFUGAL FORCE

ANALYTICAL METHOD USING CENTRIFUGAL FORCE

The present invention provides a process for carrying out analytical determinations by mixing and incubating a sample solution with at least one reagent and optically measuring a parameter in the incubated reaction mixture. The mixing, incubating and measuring are carried out during the action of a centrifugal force which forces the solution through a plurality of interconnected small hollow spaces having flow resistance so adapted with regard to one another as to mix the reaction components. Incubation may also take place before the reaction solution passes from the plurality of interconnected small hollow spaces into a measuring chamber in which the measurement is carried out.

SELECTIVE SEPARATION BY A CHROMATOGRAPHIC PROCESS

Chemical analysis

A system for separating compounds of relatively low molecular weight substantially not greater than about one kilodalton from compounds having relatively high molecular weights substantially an order of magnitude greater or more than the low molecular weight compounds in a liquid mixture. The system includes a chromatographic column packed with uniformly distributed rigid, solid, porous particles having chromatographically active, hydrophobic surfaces, average diameters of not less than about 30 μm, and average pore diameters sufficiently small to substantially exclude introduction of the compounds of relatively high molecular weight into the pores. The mixture is pumped through the interstitial volume between the particles at a reduced velocity greater than about 5,000, until a band of the high molecular weight compounds exits the column. The low molecular weight compounds are then eluted and are recovered separately from the relatively high molecular weight compounds. Spectrographic identification ...

Packed columns having a small diameter for gas chromatography

The partitioning material in a gas chromatography column has a dimension greater than 1/5 of the inner diameter e.g. 1 mm. or less, of the column. The total solid volume of the particles may be less than 55% of the inner volume of the tube and the particles may be coated with a partitioning liquid whilst the particles themselves may act as the sorbing material. The particles may be packed in the column to form alternate zones of packed and unpacked column by loosely packing a tubular column having an inner diameter in excess of the inner diameter of the ultimate column, with granular partitioning material and drawing the column down to the desired size. The particles for packing such a column may have a diameter greater than 1/5 of the diameter of the ultimate column. A rod may be placed in the tube before packing and withdrawn prior to the reduction in size thereof thus causing the alternate packed and unpacked zones to be formed. The partitioning material may be Al2O3, whilst the tube ...

HIGH PERFORMANCE LIQUID CHROMATOGRAPHY METHOD AND APPARATUS

A method of and apparatus for liquid chromatography at improved analytical and preparative speeds and quantities involves flow of fluids through the column at flow rates sufficient to induce turbulent flow in those fluids. In one embodiment, the apparatus and method includes a substantially uniform chromatography column created by packing together a multiplicity of rigid, solid, porous particles having diameters of not less than about 30 .mu.m, surfaces of the particles being chromatographically active. A fluid mixture containing at least one solute that is reactive with the particle surfaces is injected into the column and subsequently eluted therefrom by an eluant fluid flow, both the injection and elution being effected at a velocity sufficient to induce flow within at least a substantial portion of the interstitial volume between the particles at a reduced velocity greater than about 5,000.

MASS SPECTROMETRY ASSAY FOR THIOPURINE-S-METHYL TRANSFERASE ACTIVITY AND PRODUCTS GENERATED THEREBY

Methods are described for measuring the amount of a methylation TPMT enzyme product in a sample. More specifically, mass spectrometric methods are described for detecting and quantifying 6-MMP or isotopically labeled 6-MMP in a test sample utilizing mass spectrometric techniques and for using such methods to determine the activity of TPMT enzyme that is present in a sample.

CHROMATOGRAPHIC PROCESSES AND APPARATUS

HOCHLEISTUNGSFLÜSSIGKEITSCHROMATOGRAPHIEVERFAHREN UND VORRICHTUNG

SEPARATING COLUMN FOR GAS CHROMATOGRAPHY

SEPARATING COLUMN FOR GAS CHROMATOGRAPHY A method of fabricating an elution chromatography separating column from an elongated capillary tube, such column having a layer comprising a plurality of disunited particles of porous solid material disposed on an inner surface of the tube. The method comprises the steps of: preparing a suspension comprising a dispersive liquid and particles of the solid material; filling an elongated capillary tube with the prepared suspension; and flowing an inert gas through the tube to dry the tube thereby leaving a layer comprising a plurality of disunited particles of the solid material attached to the inner surface of the tube. DCRH04/MF ...

Columns furnished for the chromatography

Process and measuring device of at least a variable of constitution and concentration of a chromatographic éluat.

Process and apparatus of chromatography

A TURBULENT FLOW MIXING DEVICE FOR USE IN A CHROMATOGRAPHY SYSTEM

A mixing device for use in a chromatography system, the device includes an exterior housing having a first end and a second end and a hydraulic flow connector at the first end of the exterior housing. A cartridge including a chamber is enclosed within the exterior housing. The chamber has at least one wall defining an interior volume having a shape, wherein the shape of the interior volume creates a turbulent flow condition to mix at least two fluids and provide flow through the cartridge during operation of the chromatography system. The chamber also retains the sample, thereby ensuring that the sample is focused in the chamber such that a more narrow bolus of sample enters the chromatography column.

SEQUENTIAL DETECTION ION CHROMATOGRAPHY

Packings or equivalent structures which produce radial mixing in channels can be used to enhance reagent addition devices for ion chromatography post column reactions in which band spreading at detrimental levels is effectively controlled. Application to two dimensional conductometric detection in simultaneous suppressed and nonsuppressed ion chromatography.

Separating column for gas chromatography

Improvements in Chromatographic Processes and Apparatus.

1,183,833. Gas and liquid chromatography. V. PRETORIUS and H. H. HAHN. 9 Aug., 1967 [11 Aug., 1966], No. 36538/67. Addition to 1148661. Headings B1L and B1X. A gas or liquid chromatographic separating process is carried out in a tubular column in which movement of the retarding phase is stationary or restrained and the relative overall flow velocity between the phases is adjusted to a value in excess of the velocity corresponding to the onset in the forwarding phase of convective flow phenomena apparent by a point of downward inflection in a graphical representation of plate height against the said relative overall velocity, and the chromatographic separation is carried out at this adjusted velocity. In Fig 1, the ordinate h is proportional to the plate height, the abscissa Re is proportional to the relative overall velocity, and the point of downward inflection is at c. The separation may be carried out at or beyond a relative overall velocity corresponding to point d. Columns described may be of copper internally covered with whiskers or fur formed by oxidation with sodium chlorite in the presence of cyanide ion and strong alkali (Fig. 2); have an internal spiral groove for countercurrent chromatography (Fig. 6); have inclined perforated plates (Fig. 8); comprise bonded beads coated with retarding phase (Fig. 11) or Raschig rings (Fig. 12).

Chromatographic Separation

... 1,194,376. Chromatography. V. PRETORIUS and. H. H. HAHN. 25 July, 1967 [2 Aug., 1966], No. 34180/67. Heading B1X. In a liquid chromatographic separating process, retention of solute ions, amendable to reversable electrodeposition is induced by applying an alternating current potential difference having a puke-shape of diminishing width at increasing potential levels 'between a reference locality and a locality on the retarding phase. The pulse shapes may be as in Fig. 4, may be imposed on a direct current potential, and may be applied between either end of the retarding phase, or (as in Fig. 1, not shown) between electrically pervious packing 24, Fig. 5 and electrode material 25 which forms the reference locality. Specifications 1,169,523 and 1,120,364 are referred to.

MODIFIED MEMBRANE CHROMATOGRAPHIC ELEUNT SUPPRESSOR

SEPARATING COLUMN FOR GAS CHROMATOGRAPHY

SELECTIVE SEPARATION BY A CHROMATOGRAPHIC PROCESS

METHOD AND APPARATUS FOR FRACTIONAL SEPARATION OF MIXTURE

Modular multi-column chromatographic box

一种模块式多柱色谱盒，即用于色谱分离的盒包括一个壳体；至少部分地穿过该壳体的至少一个第一和一个第二色谱柱，每个色谱柱包括一个第一和一个第二端接头，这些端接头可操作以便将该色谱连接至一个色谱系统的外部管线。在每个柱的各端处突出在该壳体之外的多个连接接头实现与一个色谱管道系统和/或质谱仪检测器的流体连接。该盒可以包括多个加热器和多个温度传感器以及任选的多个传感器以便监测流体流动速率或pH。一个无源鉴别特征，例如，一个条形码或RFID模块，可以鉴别该盒和它的针对外部软件的相关色谱方法。此外，一个板载记忆模块和控制器芯片可以用于主动地记录计算机可读模块信息，包括模块历史信息，该信息可通过一个标准接口如一个通用串行总线（USB）端口传送。

Process and apparatus for the introduction of samples into systems of chromatographic separation

HIGH POROSITY COLUMN FOR GAS CHROMATOGRAPHY

Microfluidic chromatography

The present invention is directed to a microfluidic chromatography apparatus comprising a microfabricated fluid delivery system and a chromatography column which is in fluid communication with the fluid delivery system, and a method for producing and using the same. Preferably, the chromatography column comprises an OTLC, PCLC, or combinations thereof.

Detektionsverfahren und -vorrichtung

Control of sample separation based on analysis of mobile phase supply from mobile phase container

Gepackte Trennkolonnen geringen Durchmessers fuer die Fluessigkeitschromatographie

Chemical analyses

A system for separating compounds of relatively low molecular weight substantially not greater than about one kilodalton from compounds having relatively high molecular weights substantially an order of magnitude greater or more than the low molecular weight compounds in a liquid mixture. The system includes a chromatographic column packed with uniformly distributed rigid, solid, porous particles having chromatographically active, hydrophobic surfaces, average diameters of not less than about 30 mu m, and average pore diameters sufficiently small to substantially exclude introduction of the compounds of relatively high molecular weight into the pores. The mixture is pumped through the interstitial volume between the particles at a reduced velocity greater than about 5,000, until a band of the high molecular weight compounds exits the column. The low molecular weight compounds are then eluted and are recovered separately from the relatively high molecular weight compounds. Spectrographic ...

MASS SPECTROMETRY ASSAY FOR THIOPURINE-S-METHYL TRANSFERASE ACTIVITY AND PRODUCTS GENERATED THEREBY

Methods are described for measuring the amount of a methylation TPMT enzyme product in a sample. More specifically, mass spectrometric methods are described for detecting and quantifying 6-MMP or isotopically labeled 6-MMP in a test sample utilizing mass spectrometric techniques and for using such methods to determine the activity of TPMT enzyme that is present in a sample.

High performance liquid chromatography method and apparatus

A method of and apparatus for liquid chromatography at improved analytical and preparative speeds and quantities involves flow of fluids through the column at flow rates sufficient to induce turbulent flow in those fluids. In one embodiment, the apparatus and method includes a substantially uniform chromatography column created by packing together a multiplicity of rigid, solid, porous particles having diameters of not less than about 30 mu m, surfaces of the particles being chromatographically active. A fluid mixture containing at least one solute that is reactive with the particle surfaces is injected into the column and subsequently eluted therefrom by a eluant fluid flow, both the injection and elution being effected at a velocity sufficient to induce flow within at least a substantial portion of the interstitial volume between the particles at a reduced velocity greater than about 5,000.

[UNK]

TURBULENT FLOW MIXING DEVICE FOR USE IN A CHROMATOGRAPHY SYSTEM

A mixing device for use in a chromatography system, the device includes an exterior housing having a first end and a second end and a hydraulic flow connector at the first end of the exterior housing. A cartridge including a chamber is enclosed within the exterior housing. The chamber has at least one wall defining an interior volume having a shape, wherein the shape of the interior volume creates a turbulent flow condition to mix at least two fluids and provide flow through the cartridge during operation of the chromatography system. The chamber also retains the sample, thereby ensuring that the sample is focused in the chamber such that a more narrow bolus of sample enters the chromatography column.

IMPROVEMENT IN CHEMICAL ANALYSES

A system for separating compounds of relatively low molecular weight substantially not greater than about one kilodalton from compounds having relatively high molecular weight substantially an order of magnitude greater or more than the low molecular weight compounds in a liquid mixture. The system includes a chromatographic column (20) packed with uniformly distributed rigid, porous particles (22) having chromatographically active, hydrophobic surfaces, average diameters of not less than about 30 νm, and average pore diameters sufficiently small to substantially exclude introduction of the compounds of relatively high molecular weight into the pores. The mixture is pumped through the interstitial volume between the particles (22) at a reduced velocity greater than about 5,000, until a band of the high molecular weight compounds exits the column (20). The low molecular weight compounds are then eluted and are recovered separately from the relatively high molecular weight compounds. Spectrographic identification of the recovered low molecular weight compounds can then be made, preferably by ultraviolet absorption or by mass spectrograph.

VERFAHREN ZUR DURCHFUEHRUNG ANALYTISCHER BESTIMMUNGEN UND HIERFUER GEEIGNETES ROTOREINSATZELEMENT

High-performance liquid chromatography

A chromatographic column assembly is described, comprising a channel and a flow inducer. The channel comprises an inlet for receiving a sample liquid and an outlet, and is adapted for separating the sample liquid into components when the sample liquid flows through the channel in an axial direction from the inlet to the outlet. The flow inducer is adapted for controllably inducing, when the sample fluid is flowing through the channel, a motion of the sample liquid in the channel in a plane substantially orthogonal to the axial direction.

Mass spectrometry assay for thiopurine-S-methyl transferase activity and products generated thereby

Methods are described for measuring the amount of a methylation TPMT enzyme product in a sample. More specifically, mass spectrometric methods are described for detecting and quantifying 6-MMP or isotopically labeled 6-MMP in a test sample utilizing mass spectrometric techniques and for using such methods to determine the activity of TPMT enzyme that is present in a sample.

VORRICHTUNG UND VERFAHREN ZUM REINIGEN EINES ELUIERMITTELS

Microfluidic chromatography

The present invention is directed to a microfluidic chromatography apparatus comprising a microfabricated fluid delivery system and a chromatography column which is in fluid communication with the fluid delivery system, and a method for producing and using the same. Preferably, the chromatography column comprises an OTLC, PCLC, or combinations thereof.

DETECTION IN CHROMATOGRAPHY

Microfluidic chromatography

The present invention is directed to a microfluidic chromatography apparatus comprising a microfabricated fluid delivery system and a chromatography column which is in fluid communication with the fluid delivery system, and a method for producing and using the same. Preferably, the chromatography column comprises an OTLC, PCLC, or combinations thereof.

System with reusable column with resin packing and unpacking procedures and sample analysis

Systems and methods for automatically packing resin into and unpacking resin from a reusable separation column for sample analysis are described. A method embodiment includes, but is not limited to, introducing a slurry of resin to a reusable sample separation column to pack the reusable column with resin; introducing a sample solution to the reusable sample separation column; and unpacking the resin from the reusable sample separation column with a flow of liquid unpacking reagent and gaseous material.

Weiterbildung eines chromatografischen Verfahrens und Vorrichtungen

HOCHLEISTUNGSFLÜSSIGCHROMATOGRAPHIEVERFAHREN UND VORRICHTUNG

MODIFIZIERTER MEMBRANUNTERDRUECKER UND ANWENDUNGSVERFAHREN.

SEPARATING COLUMN FOR GAS CHROMATOGRAPHY

MODULAR MULTIPLE-COLUMN CHROMATOGRAPHY CARTRIDGE

A cartridge for chromatography separations comprises a housing; at least a first and a second chromatography column at least partially passing through the housing, each chromatography column comprising a first and a second end fitting operable to connect the chromatography to external tubing of a chromatography system. Connection fittings protruding outside of the housing at each end of each column enable fluidic connection to a chromatograph plumbing system and/or mass spectrometer detector. The cartridge may comprise heaters and temperature sensors and optional sensors to monitor fluid flow rate or pH. A passive identification feature, e.g., a barcode or RFID module may identify the cartridge and its associated chromatography methods to external software. Further, an on-board memory module and controller chip may be used to actively record computer-readable module information, including module history information, the information transferable through a standard interface, such as a universal serial bus (USB) port.

METHODS AND DEVICES FOR MICROFLUIDIC EXTRACTION

Devices for performing liquid extraction of one or more constituents from one fluid to another fluid are provided. In operation, the fluids are separated by channel structures that stabilize the interfacial boundary between the fluids allowing, for example, countercurrent flow and exchange or other flow conditions incompatible with unassisted maintenance of laminar flow. Also provided are channel structures which aid in mixing the fluids. Thin membranes may be formed using liquid extraction devices according to the invention. A process for manufacturing such devices using DRIE is described.

Verbesserungen in chromatographischen Verfahren und Vorrichtungen

Modular Multiple-Column Chromatography Cartridge

A method for liquid chromatography comprises steps of: mixing a sample comprising a plurality of chemical constituents with a first solvent; transferring the solvent and the plurality of constituents to a first chromatographic column housed in a cartridge; chromatographically separating a sub-group of the plurality of constituents from unwanted constituents using the first chromatographic column; transferring the sub-group of the plurality of constituents from the first chromatographic column to a second column housed in the cartridge, the transferring effected by the flow of a second solvent; chromatographically separating, from one another, individual constituents of the sub-group of the plurality of constituents using the second chromatographic column; and transferring the separated individual constituents to a detector. 1mixing a sample comprising a plurality of chemical constituents with a first solvent;transferring the solvent and the plurality of constituents to a first chromatographic column housed in a cartridge;chromatographically separating a sub-group of the plurality of constituents from unwanted constituents using the first chromatographic column;transferring the sub-group of the plurality of constituents from the first chromatographic column to a second column housed in the cartridge, the transferring effected by the flow of a second solvent;chromatographically separating, from one another, individual constituents of the sub-group of the plurality of constituents using the second chromatographic column; andtransferring the separated individual constituents to a detector.. A method for liquid chromatography comprising: This application is a Divisional application of U.S. patent application Ser. No. 13/882,116, having a 371(c) date of May 2, 2013, which is the United States National Stage application, under 35 U.S.C. 371, of International Application PCT/US2011/058229 having an international filing date of Oct. 28, 2011, which claims the benefit of the ...

HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY

A chromatographic column assembly is described, comprising a channel and a flow inducer. The channel comprises an inlet for receiving a sample liquid and an outlet, and is adapted for separating the sample liquid into components when the sample liquid flows through the channel in an axial direction from the inlet to the outlet. The flow inducer is adapted for controllably inducing, when the sample fluid is flowing through the channel, a motion of the sample liquid in the channel in a plane substantially orthogonal to the axial direction. 117.-. (canceled)18. A chromatographic column assembly comprising:a channel comprising an inlet for receiving a sample liquid and an outlet, the channel being adapted for separating the sample liquid into components when said sample liquid flows through the channel in an axial direction from said inlet to said outlet; anda controllable flow inducer for inducing, when the sample fluid is flowing through the channel, a motion of said sample liquid in the channel in a plane substantially orthogonal to said axial direction.19. The chromatographic column assembly according to claim 18 , wherein said controllable flow inducer is a vortex flow inducer.20. The chromatographic column assembly according to claim 18 , wherein said controllable flow inducer comprises a plurality of electrodes arranged in or on said channel claim 18 , the plurality of electrodes being adapted for generating an electric field suitable for inducing said motion.21. The chromatographic column assembly according to claim 18 , wherein said controllable flow inducer comprises a plurality of electrodes arranged in or on said channel claim 18 , the plurality of electrodes inducing a motion by acting on charged and/or polarizable constituents of said sample liquid claim 18 , independent of the macroscopic flow of the sample liquid.22. The chromatographic column assembly according to claim 20 , further comprising an electrical insulator for electrically insulating said ...

Control of sample separation based on analysis of mobile phase supply from mobile phase container

A method of controlling a sample separation apparatus, for separating a fluidic sample using a mobile phase provided from at least one mobile phase container, includes determining a weight and volume reduction behavior according to which weight and volume of mobile phase in a mobile phase container are reduced during conveying mobile phase from the mobile phase container in the sample separation apparatus, and determining a tare weight of the mobile phase container based on a gross weight information, a volume information, and the determined weight and volume reduction behavior. The gross weight information is indicative of an initial gross weight of the mobile phase container including its mobile phase, and the volume information is indicative of an initial mobile phase volume in the mobile phase container.

High-performance liquid chromatography with a vortex flow inducer

A chromatographic column assembly (2) is described, comprising a channel as the chromatographic column (12) and a vortex flow inducer (5). The channel (12) comprises an inlet (13) for receiving a sample liquid and an outlet (14), and is adapted for separating the sample liquid into components when the sample liquid flows through the channel in an axial direction from the inlet to the outlet. The vortex flow inducer (5) is adapted for inducing, when the sample fluid is flowing through the channel, a motion (19) of the sample liquid in the channel in a plane substantially orthogonal to the axial direction (A).

Separating column for performing gas chromatographic analyzes

METHOD AND APPARATUS FOR THE FRACTIONAL SEPARATION OF MIXTURES

Turbulent mixing device for use in chromatography systems

Microfluidic chromatography

The present invention is directed to a microfluidic chromatography apparatus comprising a microfabricated fluid delivery system and a chromatography column which is in fluid communication with the fluid delivery system, and a method for producing and using the same. Preferably, the chromatography column comprises an OTLC, PCLC, or combinations thereof.

Hochleistungsflüssigchromatographieverfahren und vorrichtung

一种新型燃烧在线离子色谱系统及其使用方法

本发明公开了一种新型燃烧在线离子色谱系统及其使用方法，系统包括工作台、加热炉、第一驱动单元、密封单元和自动进样器，所述工作台上设有依次连接的燃烧管、样品舟室和导向管，所述燃烧管包括内管和外管，所述外管的第一端与内管外壁面通过封口结构连接，所述外管的第二端形成锥形接口，所述内管的第一端与样品舟室连通，所述内管的第二端伸入至外管内；第二驱动单元驱动密封筒滑移以打开或密封上进料口和下出料口，自动进样器可完成进样操作。本发明结构合理、自动上下料效力高且有利于提高检测结果准确性可靠性。

一种新型燃烧在线离子色谱系统及其使用方法

本发明公开了一种新型燃烧在线离子色谱系统及其使用方法，系统包括工作台、加热炉、第一驱动单元、密封单元和自动进样器，所述工作台上设有依次连接的燃烧管、样品舟室和导向管，所述燃烧管包括内管和外管，所述外管的第一端与内管外壁面通过封口结构连接，所述外管的第二端形成锥形接口，所述内管的第一端与样品舟室连通，所述内管的第二端伸入至外管内；第二驱动单元驱动密封套滑移以打开或密封上进料口和下出料口，自动进样器可完成进样操作。本发明结构合理、自动上下料效力高且有利于提高检测结果准确性可靠性。

Optical detector flow cell for co2-based chromatography

The present disclosure relates to optical flow cells for use in chromatography or extraction systems. The optical flow cells include an inlet portion configured to receive a highly compressible fluid and an inlet transition portion or gasket having an internal volume and internal geometry configured to receive the highly compressible fluid from the inlet portion. The optical flow cell also includes an optical path portion configured to receive the highly compressible fluid from the inlet transition portion and direct the highly compressible fluid along an optical flow path. The internal volume and the internal geometry of the inlet transition portion configured to minimize turbulence and eddies within the highly compressible fluid as it travels through the optical flow path.

Analysis of mobile phase supply from mobile phase container

In a sample separation apparatus for separating a fluidic sample, a mobile phase container is identified by determining a weight and volume characteristic of the mobile phase container, and identifying the mobile phase container based on a comparison of the determined weight and volume characteristic of the mobile phase container with a pre-known reference weight and volume characteristic of one or more reference mobile phase containers with pre-known identity. A mobile phase is identified by determining a weight and volume reduction behavior according to which weight and volume of mobile phase in a mobile phase container are reduced during conveying mobile phase from the mobile phase container in the sample separation apparatus, and identifying the mobile phase based on a comparison of the determined weight and volume reduction behavior with pre-known reference weight and volume reduction behavior of one or more reference mobile phase materials with pre-known identity.

Mass spectrometry assay for thiopurine-s-methyl transferase activity and products generated thereby

Methods are described for measuring the amount of a methylation TPMT enzyme product in a sample. More specifically, mass spectrometric methods are described for detecting and quantifying 6-MMP or isotopically labeled 6-MMP in a test sample utilizing mass spectrometric techniques and for using such methods to determine the activity of TPMT enzyme that is present in a sample.

基于来自流动相容器的流动相供应的分析来控制样品分离

一种控制样品分离装置(10)的方法，该样品分离装置(10)用于使用从至少一个流动相容器(100)提供的流动相(I、II)来分离流体样品，其中该方法包括：确定重量和体积减少行为(132)，在样品分离装置(10)中从流动相容器(100)输送流动相(I、II)期间，流动相容器(100)中流动相(I、II)的重量和体积根据该行为而减少，以及基于总重量信息、体积信息以及所确定的重量和体积减少行为(132)来确定流动相容器(100)的皮重(138)，其中总重量信息指示流动相容器(100)在包括其流动相(I、II)的情况下的初始总重量(128)，体积信息指示流动相容器(100)中的初始流动相体积(130)。

High performance liquid chromatography method and apparatus

A method of and apparatus for liquid chromatography at improved analytical and preparative speeds and quantities involves flow of fluids through the column at flow rates sufficient to induce turbulent flow in those fluids. In one embodiment, the apparatus and method includes a substantially uniform chromatography column created by packing together a multiplicity of rigid, solid, porous particles having diameters of not less than about 30 mu m, surfaces of the particles being chromatographically active. A fluid mixture containing at least one solute that is reactive with the particle surfaces is injected into the column and subsequently eluted therefrom by a eluant fluid flow, both the injection and elution being effected at a velocity sufficient to induce flow within at least a substantial portion of the interstitial volume between the particles at a reduced velocity greater than about 5,000.

High performance liquid chromatography method and apparatus

Cromatografia liquida de alto rendimiento, procedimiento y aparato.

SE DESCRIBE UN PROCEDIMIENTO Y UN APARATO PARA LA CROMATOGRAFIA LIQUIDA CON UNAS VELOCIDADES Y CANTIDADES ANALITICAS Y PREPARATIVAS MEJORADAS, QUE IMPLICA EL FLUJO DE FLUIDOS A TRAVES DE LA COLUMNA A UNA VELOCIDAD DE FLUJO SUFICIENTE PARA INDUCIR UN FLUJO TURBULENTO EN ESTOS FLUIDOS. EN UNA REALIZACION, EL APARATO Y EL PROCEDIMIENTO INCLUYEN UNA COLUMNA DE CROMATOGRAFIA SUSTANCIALMENTE UNIFORME CREADA CARGANDO UNA MULTIPLICIDAD DE PARTICULAS POROSAS, RIGIDAS Y SOLIDAS QUE PRESENTAN UNOS DIAMETROS NO MENORES QUE APROXIMADAMENTE 30 MI}M, SIENDO LAS SUPERFICIES DE LAS PARTICULAS CROMATOGRAFICAMENTE ACTIVAS. UNA MEZCLA FLUIDA QUE CONTIENE AL MENOS UN SOLUTO QUE ES REACTIVO CON LAS SUPERFICIES DE LAS PARTICULAS SE INYECTA EN LA COLUMNA Y POSTERIORMENTE SE ELUYE DE ESTA MEDIANTE UN FLUJO DE FLUIDO DE ELUCION, LLEVANDOSE A CABO LA INYECCION Y LA ELUCION A UNA VELOCIDAD SUFICIENTE PARA INDUCIR UN FLUJO DENTRO DE AL MENOS UNA PORCION SUSTANCIAL DEL VOLUMEN INTERSTICIAL ENTRE LAS PARTICULAS A UNA VELOCIDAD REDUCIDA MAYOR QUE APROXIMADAMENTE 5.000.

一种水处理工艺中微生物体内鸟苷四磷酸的hplc检测方法

本发明涉及污水处理与生化分析及检测领域，公开了一种水处理工艺中微生物体内鸟苷四磷酸的HPLC检测方法，具体为：采用低温强酸提取微生物体内核苷酸的方式，有效提取微生物体内的核苷酸类物质，然后通过选择磷酸二氢钾浓度为5～10mM、pH值为3.9～4.1的超纯水溶液作为流动相A，磷酸二氢钾浓度为0.5M、硫酸钠浓度为0.5M、pH值为5.3～5.5的超纯水溶液作为流动相B，利用高效液相色谱结合标准曲线法进行检测及计算，实现对水处理微生物中鸟苷四磷酸含量的准确测定，为完善水处理工艺中微生物生长状态调节机制研究奠定基础。

Højeffektiv væskekromatografi

Verfahren und vorrichtung zur probeneinfuehrung in chromatographische trennsysteme

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High-performance liquid chromatography with a controllable transverse flow inducer

A chromatographic column assembly (2) is described, comprising a channel (12) and a flow inducer (5). The channel (12) comprises an inlet (13) for receiving a sample liquid and an outlet (14), and is adapted for separating the sample liquid into components when the sample liquid flows through the channel in an axial direction from the inlet to the outlet. The flow inducer (5) is adapted for controllably inducing, when the sample fluid is flowing through the channel, a motion (19) of the sample liquid in the channel in a plane substantially orthogonal to the axial direction (A).

Detektorvorrichtung zum Überwachen der Zusammensetzung chromatographischer Eluate Ausscheidung aus: 1792701

Improvement in chemical analyses

A system for separating compounds of relatively low molecular weight substantially not greater than about one kilodalton from compounds having relatively high molecular weight substantially an order of magnitude greater or more than the low molecular weight compounds in a liquid mixture. The system includes a chromatographic column (20) packed with uniformly distributed rigid, porous particles (22) having chromatographically active, hydrophobic surfaces, average diameters of not less than about 30 νm, and average pore diameters sufficiently small to substantially exclude introduction of the compounds of relatively high molecular weight into the pores. The mixture is pumped through the interstitial volume between the particles (22) at a reduced velocity greater than about 5,000, until a band of the high molecular weight compounds exits the column (20). The low molecular weight compounds are then eluted and are recovered separately from the relatively high molecular weight compounds. Spectrographic identification of the recovered low molecular weight compounds can then be made, preferably by ultraviolet absorption or by mass spectrograph.

Improvement in chemical analyses

A system for separating compounds of relatively low molecular weight substantially not greater than about one kilodalton from compounds having relatively high molecular weight substantially an order of magnitude greater or more than the low molecular weight compounds in a liquid mixture. The system includes a chromatographic column (20) packed with uniformly distributed rigid, porous particles (22) having chromatographically active, hydrophobic surfaces, average diameters of not less than about 30 νm, and average pore diameters sufficiently small to substantially exclude introduction of the compounds of relatively high molecular weight into the pores. The mixture is pumped through the interstitial volume between the particles (22) at a reduced velocity greater than about 5,000, until a band of the high molecular weight compounds exits the column (20). The low molecular weight compounds are then eluted and are recovered separately from the relatively high molecular weight compounds. Spectrographic identification of the recovered low molecular weight compounds can then be made, preferably by ultraviolet absorption or by mass spectrograph.

ガスクロマトグラフイ−用分離カラム

(57)【要約】本公報は電子出願前の出願データであるた め要約のデータは記録されません。

Hochleistungsflüssigkeitschromatographieverfahr n und vorrichtung

Verfahren zur durchfuehrung analytischer bestimmungen und hierfuer geeignetes rotoreinsatzelement.

基于来自流动相容器的流动相供应的分析来控制样品分离

一种控制样品分离装置(10)的方法，该样品分离装置(10)用于使用从至少一个流动相容器(100)提供的流动相(I、II)来分离流体样品，其中该方法包括：确定重量和体积减少行为(132)，在样品分离装置(10)中从流动相容器(100)输送流动相(I、II)期间，流动相容器(100)中流动相(I、II)的重量和体积根据该行为而减少，以及基于总重量信息、体积信息以及所确定的重量和体积减少行为(132)来确定流动相容器(100)的皮重(138)，其中总重量信息指示流动相容器(100)在包括其流动相(I、II)的情况下的初始总重量(128)，体积信息指示流动相容器(100)中的初始流动相体积(130)。

一种新型燃烧在线离子色谱系统及其使用方法

本发明公开了一种新型燃烧在线离子色谱系统及其使用方法，系统包括工作台、加热炉、第一驱动单元、密封单元和自动进样器，所述工作台上设有依次连接的燃烧管、样品舟室和导向管，所述燃烧管包括内管和外管，所述外管的第一端与内管外壁面通过封口结构连接，所述外管的第二端形成锥形接口，所述内管的第一端与样品舟室连通，所述内管的第二端伸入至外管内；第二驱动单元驱动密封套滑移以打开或密封上进料口和下出料口，自动进样器可完成进样操作。本发明结构合理、自动上下料效力高且有利于提高检测结果准确性可靠性。

一种水处理工艺中微生物体内鸟苷四磷酸的hplc检测方法

本发明涉及污水处理与生化分析及检测领域，公开了一种水处理工艺中微生物体内鸟苷四磷酸的HPLC检测方法，具体为：采用低温强酸提取微生物体内核苷酸的方式，有效提取微生物体内的核苷酸类物质，然后通过选择磷酸二氢钾浓度为5～10mM、pH值为3.9～4.1的超纯水溶液作为流动相A，磷酸二氢钾浓度为0.5M、硫酸钠浓度为0.5M、pH值为5.3～5.5的超纯水溶液作为流动相B，利用高效液相色谱结合标准曲线法进行检测及计算，实现对水处理微生物中鸟苷四磷酸含量的准确测定，为完善水处理工艺中微生物生长状态调节机制研究奠定基础。

雪梨膏的指纹图谱检测方法

本发明公开了雪梨膏的指纹图谱检测方法，该方法包括以下步骤：步骤1、雪梨膏供试品溶液的制备；步骤2、混合对照品溶液的制备：步骤3、分别精密吸取供试品溶液和混合对照品溶液注入高效液相色谱仪，记录色谱图；步骤4，将步骤3中获得的雪梨膏指纹图谱仪器导出，并导入中药色谱指纹图谱相似度评价系统，选择不同批次雪梨膏的色谱图中均存在的色谱峰作为共有峰；用平均值计算法生成雪梨膏的对照指纹图谱；计算各共有峰的相对保留时间、相对峰面积。本发明所提供的雪梨膏指纹图谱，能全面，客观地表征雪梨膏的质量。本发明提供的指纹图谱的检测方法具有方法简便、稳定、精密度高、重现性好等优点。