Mikrofluidische Glykananalyse

Es werden Mikrofluideinheiten und -verfahren zum Analysieren der Glykanprofile von Glykoproteinen bereitgestellt. Einige Ausführungsformen der Mikrofluideinheiten weisen eine Deglykosylierungs-Säule zum Abspalten der Glykane, wahlweise eine Reinigungssäule zum Entfernen der Proteine, eine Trap-Säule zum Anreichern der Glykane und eine Trennsäule zum Trennen der Glykane auf. Die Mikrofluideinheiten und -verfahren verbessern die Geschwindigkeit und das Auflösungsvermögen der Glykananalyse beträchtlich.

Verfahren und Vorrichtung zum Trennen von Teilchen

MONODISPERSE PARTIKEL VERWENDENDE KAPILLARSÄULEN

Elucidating the metabolism of substances, useful for investigating the degradation of pharmaceuticals, comprises analyzing substances in a liquid reaction system to identify the metabolites by liquid chromatography and mass spectrometry

Elucidating the metabolism of substances, comprising analyzing substances in a liquid reaction system to determine the decomposition product, is new. Elucidating the metabolism of substances, comprising analyzing substances in a liquid reaction system to determine the decomposition products, is new. Potential decomposition products are computed in advance according to a set of degrading rules matching the effects of enzymes, to set the conditions for identification in the analysis procedure. The analysis uses a combination of chromatography to separate the liquid components to be identified by mass spectrometry.

Stacked layer-type member with integrated functional component

A fluidic valve 90 for a sample separation apparatus (10) for separating a fluid, wherein the fluidic valve 90 comprises a stack of connected layer structures 200, a first conduit 202 within the stack, a second conduit 204 within the stack, a movable body 206 within the stack, and an actuator 208 configured for actuating the movable body 206 to selectively bring the movable body 206 into a flow enabling configuration in which flow of fluid between the first conduit 202 and the second conduit 204 is enabled, or into a flow disabling configuration in which flow of fluid between the first conduit 202 and the second conduit 204 is disabled. Also claimed is a method of manufacturing fluidic valve 90. Also claimed is a planar member comprising a stack of metallic layer structures connected to one another and at least one non-metallic functional component integrated with the stack.

Microfluidic flame ionization detector

Minature HPLC device

GAS CHROMATOGRAPH

A gas chromatograph (10) having a plurality of layers or channels comprises an inlet (12), a column (16) disposed in each of the chromatograph layers, each column being in fluid communication with and downstream from the inlet and having a stationary phase coating its inner surfaces, and a detector (18) in fluid communication with and downstream from at least one of the columns. The chromatograph includes a pre-column (14) disposed in each of the chromatograph layers upstream of columns 16, each pre-column being in fluid communication with the inlet and having a stationary phase coating its inner surfaces. Heaters 18, 20 may provide a thermal gradient across all the columns so that different weight fractions may elute from each column during the same time span. Heater 18 may be a thermoelectric cooler. Pre-columns 14 may be purged while the sample passes through main columns 16 via lines 82, 84, 86.

Gas chromatograph

Fluid transport system

MINIATURIZED GAS CHROMATOGRAPH AND INJECTOR FOR THIS

VERFAHREN UND VORRICHTUNG ZUR VORHERSAGE EINES DESTILLATIONSTEMPERATURBEREICHS EINER KOHLENWASSERSTOFFE ENTHALTENDEN VERBINDUNG

SYSTEM FOR THE TRANSPORT OF MICROSCOPIC LIQUID QUANTITIES

MESSEINRICHTUNGEN UND ROHRANSCHLUSS SOWIE VERFAHREN ZUR HERSTELLUNG EINER MESSEINRICHTUNG UND VERFAHREN ZUR VERBINDUNG VON ROHREN MIT EINER MESSEINRICHTUNG BZW. ZUR HERSTELLUNG VON ROHRANSCHLUESSEN

PROCEDURE AND DEVICE FOR DIFFUSION EXCHANGE BETWEEN NOT MIXABLE LIQUIDS

PROCEDURE FOR THE PRODUCTION OF INTEGRATIONS OF MULTILAYER MICRO FLUID CONSTRUCTION UNITS

DEVICE AND PROCEDURE FOR MOVING FLUIDS OF MEANS CENTRIFUGAL ACCELERATION DURING THE AUTOMATIC LABORATORY TREATMENT

METHOD FOR THE CONNECTION OF A CAPILLARY WITH A MICRO FLUID DEVICE

LOW POWER GAS LEAK DETECTOR

SYSTEM AND METHOD FOR PERFORMING MULTIPLE PARALLEL CHROMATOGRAPHIC SEPARATIONS

Method for continuous particle separation using obstacle arrays asymmetrically aligned to fields

Fabrication of ultra-shallow channels for microfluidic devices and systems

A microfabricated device and method for multiplexed electrokinetic focusing of fluid streams and a transport cytometry method using same

Micropump with sonic energy generator

Non-fouling, flow-through capacitor, system and method of separation

Method and apparatus for diffusive transfer between immiscible fluids

Method of establishing at least one enveloped flow in a channel

Sample injector for high pressure liquid chromatography

STRUCTURAL UNITS THAT DEFINE FLUIDIC FUNCTIONS

A microfluidic device that comprises several microchannel structures in which there are an inlet port, an outlet port and therebetween a substructure comprising a fluidic function. The device has an axis of symmetry around which the microchannel structures are arranged as two or more concentric annular zones. for an inlet port and an outlet port of the same microchannel structure the inlet port is typically closer to the axis of symmetry than the outlet port. Each microchannel structure comprises a substructure that can retain liquid while the disc is spun around the axis and/or the inlet ports are positioned separate from the paths waste liquid leaving open waste outlet ports will follow across the surface of the disc when it is spun. For the microchannel structures of an annular zones the corresponding substructures are at essentially at the same radial distance while corresponding substructures in microchannel structures of different annular zones are at different radial distances. The ...

INTEGRATED MULTILAYERED MICROFLUIDIC DEVICES AND METHODS FOR MAKING THE SAME

A multilayered microfluidic device having a substantially monolithic structure is formed by sintering together a plurality of green-sheet layers. The substantially monolithic structure has an inlet port for receiving fluid, an outlet port for releasing fluid, and an interconnection between the inlet port and the outlet port. The substantially monolithic structure may also include a variety of components to enable useful interaction with the fluid, such as electrically conductive pathways, heaters, fluid sensors, fluid motion transducers, and optically transmissive portions. The components are preferably fabricated using thick-film or green-sheet technology and are preferably co-fired with and sintered to the green-sheet layers to become integral with the substantially monolithic structure. By using an adhesive to bind the green-sheet layers together, the multilayered microfluidic device may be fabricated without the application of high pressures. Selection of an adhesive with a polymer ...

CONTINUOUSLY REGULATED PRECISION PRESSURE FLUID DELIVERY SYSTEM

A fluid flow characteristic regulator (58) which provides a variable volume flow path in which a fluid flow can be continuously adjusted by a control fluid (57) to regulate at least one fluid flow characteristic of the fluid flow (11) within the variable volume flow path.

CHARACTERIZATION OF REACTION VARIABLES

A microscale method for the characterization of one or more reaction variables that influence the formation or dissociation of an affinity complex comprising a ligand and a binder, which have mutual affinity for each other. The method is characterized in comprising the steps of: (i) providing a microfluidic device comprising a microchannel structures that are under a common flow control, each microchannel structure comprising a reaction microactivity; (ii) performing essentially in parallel an experiment in each of two or more of the plurality of microchannel structures, the experiment in these two or more microchannel structures comprising either a) formation of an immobilized form of the complex and retaining under flow conditions said form within the reaction microactivity, or b) dissociating, preferably under flow condition, an immobilized form of the complex which has been included in the microfluidic device provided in step (i), at least one reaction variable varies or is uncharacterized ...

VERY SMALL CHEMICAL DEVICE AND FLOW RATE ADJUSTING METHOD THEREFOR

A very small chemical device with a valve function, having high pressure- resistance and a flow channel cross-sectional area independent of liquid pressure, capable of suppressing adsorption of living body components, and easy to manufacture; and a flow rate adjusting method therefor. A member (A) having a groove in its surface has another member (B) bonded to the grooved surface thereof, and the groove of the member (A) cooperates with the member (B) to define a capillary flow channel having a width of 1-1000 m and a height of 1-1000 m on the bond surface between the members (A, B), the flow channel having a gap somewhere therein, the width of the gap being 0.5 100 times that of the capillary flow channel, the maximum height/maximum width ratio of the gap being 1 or less, either the member (A) or the member (B) being made of a soft material having a Young's modulus of from 0.1 Mpa to less than 700 Mpa at least in the portion opposed to the gap, the valve function being such that selectively ...

SOFTWARE FOR MICROFLUIDIC SYSTEMS INTERFACING WITH MASS SPECTROMETRY

Methods, devices, and systems for improving the quality of electrospray ionization mass spectrometer (ESI-MS) data are described, as are methods, devices, and systems for achieving improved correlation between chemical separation data and mass spectrometry data.

SYSTEM AND METHOD OF PRECONCENTRATING ANALYTES IN A MICROFLUIDIC DEVICE

A method and system for preconcentrating analytes at a microvalve in a microfluidic device (1600) is disclosed. The system includes a sample channel (1610) loaded with a sample solution. The sample channel (1610) includes a semi-permeable membrane microvalve (1630). An electric potential is applied at or across the microvalve (1630) to preconcentrate the sample solution when the microvalve is closed. Sample solution is subsequently injected into the separation channel (1640,2105). A tapered capillary end (2110) is inserted into the separation channel (2105). Also disclosed are methods including pretreatments of the device or valve for preconcentration of the analytes. For preconcentration of anionic analytes, the device is baked. For preconcentration of the cationic analytes, the surface of the membrane microvalve (1630) is coated with a polycationic coating, and the device is baked.

PRODUCTION OF CHEMICAL REACTORS

A method for producing a chemical reactor. The chemical reactor comprises one or more effective channels which comprise pillar structures, an input connected to one of the effective channels to allow fluid/gas into the effective channels and an output connected to one of the effective channels to remove at least one component of the liquid/gas. The method comprises obtaining an initial design of the reactor, further introducing into the initial design at least a structured area positioned adjacent to an effective channel of the one or more effective channels located at the edge of the initial design, the structured area not being fluidly connected to one of the effective channels, to obtain a further design and the production of the reactor according to the further design.

A MICROFABRICATED DEVICE AND METHOD FOR MULTIPLEXED ELECTROKINETIC FOCUSING OF FLUID STREAMS AND A TRANSPORT CYTOMETRY METHOD USING SAME

One embodiment of the present invention provides a microchip (10) adapted for the simultaneous spatial confinement of electrokinetically driven fluidic material streams on the microchip. The microchip includes a focusing chamber (22) formed in the surface of the microchip and in fluid communication with two sample channels (28, 30) and three focusing channels (32, 40, 42). The embodiment further includes electromotive means operatively connected to the sources of the sample fluids (14, 18) and the sources of focusing fluid (12, 16, 20) for electrokinetically driving the respective streams of the sample and focusing fluids such that the focusing fluid streams spatially confine the first and second sample streams within the focusing chamber. Another embodiment of the present invention provides a cytometry method for analyzing microscopic particles in a fluid medium on a microchip by utilizing the focusing function of the microchip. In this process the width of the fluid stream is narrowed ...

SEPARATOR AND METHOD OF MANUFACTURING A SEPARATOR

A separator has a specimen separating area comprising a number of recesses defined in an inner wall of a flow passage through which a specimen passes. For separating nucleic acid and protein, the recesses have openings with a maximum diameter of 300 nm, and are spaced apart at an average interval of 300 nm or less.

FLUIDIC DEVICES AND METHODS OF USING THEM

Certain embodiments described herein are directed to chromatography systems that include a microfluidic device configured to provide three-way switching or switching between three or more inputs or outputs. The microfluidic device can be fluidically coupled to one or more switching valves to provide for selective control of fluid flow in the chromatography system.

INTEGRATED CHEMICAL SYNTHESIZERS

A modular reactor system (see figure) and method for synthesizing chemical compounds characterized by a uniform temperature throughout the reaction mixture by use of a continuous flow reactor (100, 60, 70) under high pressure. The apparatus includes a number of generic components such as pumps, flow channels (81, 82, 83, 84), manifolds, flow restrictors, and valves. Modular reactors (100, 60, 70), separator and analyzers on an assembly board (80) provide a system where a modular reactor unit (100, 60, 70) has an internal diameter of up to 100 micrometers to optimize control of residence time within a reaction zone.

METHOD AND APPARATUS FOR DIFFUSIVE TRANSFER BETWEEN IMMISCIBLE FLUIDS

In order to facilitate diffusive transfer of an entity such as a solute between immiscible fluids and subsequent separation of the liquids without mixing, method and apparatus are disclosed having first and second flow paths (1, 2) carrying first and second immiscible fluids on opposite sides of a foraminous sheet (8) wherein the height of the apertures (10) therein are not greater than 200 micrometers (measured perpendicular to the width of the sheet and to the direction of fluid flow), and a stable interface is formed between the fluids within each aperture, with a significant amount of fluid flow immediately adjacent the interface. Diffusive transfer takes place across the interface, and subsequently the fluids flow away from the region without mixing. The width of the flow paths measured perpendicular to sheet (8) lies between 10 and 500 micrometers. The walls of each aperture may be parallel or tapered.

METHOD AND APPARATUS FOR DIFFUSIVE TRANSFER BETWEEN IMMISCIBLE FLUIDS

In order to facilitate diffusive transfer of an entity such as a solute between immiscible fluids and subsequent separation of the fluids without mixing, method and apparatus are disclosed having first and second fluid flow paths (11, 12) carrying first and second immiscible fluids, the flow paths communicating with one another in an interface region (16) in which the fluids contact one another and a stable open interface is formed. Diffusive transfer takes place across the interface, and subsequently the fluids flow away from the interface without mixing. The width of the flow paths in the interface region measured normal to the interface lies between 10 and 500 micrometres.

DEVICES AND METHODS FOR USING CENTRIPETAL ACCELERATION TO DRIVE FLUID MOVEMENT IN A MICROFLUIDICS SYSTEM WITH ON-BOARD INFORMATICS

This invention relates to methods and apparatus for performing microanalytic and microsynthetic analyses and procedures. The invention provides a microsystem platform and a micromanipulation device for manipulating the platform that utilizes the centripetal force resulting from rotation of the platform to motivate fluid movement through microchannels. The microsystem platforms of the invention are also provided having system informatics and data acquisition, analysis and storage and retrieval informatics encoded on the surface of the disk opposite to the surface containing the fluidic components. Methods specific for the apparatus of the invention for performing any of a wide variety of microanalytical or microsynthetic processes are provided.

CAPILLARY MICROVALVE

The invention provides microvalves for controlling fluid flow from microreservoirs into transfer channels using capillary valving mechanisms. This invention provides microvalving means for use in apparatus useful for performing microanalytic and microsynthetic analyses and procedures, such as microminiaturization of genetic, biochemical and chemical processes related to analysis, synthesis and purification of biological, chemical, environmental and other compounds.

fused-silica capillary screwing.

Die erfindungsgemässe Fused-Silica-Kapillarverschraubung weist einen konischen Klemmabschnitt (8) und einen davon getrennten Krimpabschnitt für eine Aussenhülse auf. Die Abschnitte wirken auf eine Zwischenhülse ein. Damit erfolgt das Abdichten der Fused-Silica-Kapillare in der Zwischenhülse durch die Verkrimpung der Aussenhülse und damit unabhängig von der Standard-Konus-Abdichtung (8).

CONTINUOUS FLOW ANALYSIS SYSTEM, IN PARTICULAR FLOW INJECTION ANALYSIS SYSTEM.

Apparatus for extraction and analysis of gases.

Die Vorrichtung zum Extrahieren und Analysieren von Gas weist einen Gaschromatographen mit einem miniaturisierten Chromatographen-Modul (30) und ein miniaturisiertes Gasextraktionsmodul (20) auf. Das Gasextraktionsmodul weist mindestens einen Gasextraktor (21, 22) auf, welcher eine gaspermeable Membran zum Extrahieren von Gas aus einer Flüssigkeit durch die Membran aufweist. Dabei ist eine Probengasabfuhrleitung des Gasextraktionsmoduls mit einer Kapillare zur Zufuhr von Probengas im Chromatographen-Modul verbunden und bildet einen Verbindungsabschnitt einer Probengasleitung. Der Verbindungsabschnitt der Probengasleitung zwischen Gasextraktionsmodul (20) und Injektor (34) des Chromatographen-Moduls (30) sind ventilfrei.

ПЛИТА ДЛЯ ПРОБ

В заявке описаны способ и устройство для анализа проб. Предлагаются плита для проб, портативное аналитическое устройство и способ анализа сернистых и/или азотистых соединений в пробе текучей среды, способ включает в себя подачу пробы текучей среды в плиту для проб, имеющую впуск для пробы, зону реакции, зону анализа и по меньшей мере одну зону разделения, плита для проб обеспечивает: (а) подачу пробы текучей среды в плиту для проб через впуск в зону реакции или необязательно в зону разделения; зона разделения разделяет пробу текучей среды на две или более фракций, по меньшей мере одна из которых поступает в зону реакции; (б) подачу реагента в зону реакции; (в) поддержание зоны реакции в условиях, обеспечивающих протекание реакции между реагентом и пробой текучей среды или её фракцией для получения продуктовой текучей среды и (г) перенос продуктовой текучей среды в зону анализа или необязательно в зону разделения, где продуктовая текучая среда разделяется на две или более фракций, по меньшей ...

ПЛИТА ДЛЯ ПРОБ

В изобретении описаны способ и устройство для анализа проб. Предлагаются плита для проб, портативное аналитическое устройство и способ анализа сернистых и/или азотистых соединений в пробе текучей среды, способ включает в себя подачу пробы текучей среды в плиту для проб, имеющую впуск для пробы, зону реакции, зону анализа и по меньшей мере одну зону разделения, плита для проб обеспечивает: (а) подачу пробы текучей среды в плиту для проб через впуск в зону реакции или необязательно в зону разделения; зона разделения разделяет пробу текучей среды на две или более фракций, по меньшей мере одна из которых поступает в зону реакции; (б) подачу реагента в зону реакции; (в) поддержание зоны реакции в условиях, обеспечивающих протекание реакции между реагентом и пробой текучей среды или её фракцией для получения продуктовой текучей среды и (г) перенос продуктовой текучей среды в зону анализа или необязательно в зону разделения, где продуктовая текучая среда разделяется на две или более фракций, по ...

DEVICE COMPRISING A FLUID CHANNEL HAVING AT LEAST ONE MICROELECTRONIC SYSTEM OR NANOELECTRONIC AND METHOD FOR MAKING SAME

MEASUREMENT SYSTEM INCLUDING A RESONATOR ARRAY SUCH AS NANO-ELECTROMECHANICAL SYSTEM

DEVICE COMPRISING A FLUID CHANNEL HAVING AT LEAST ONE MICROELECTRONIC SYSTEM OR NANOELECTRONIC AND METHOD FOR MAKING SAME

Dispositif comportant un substrat comprenant au moins une structure microélectronique et/ou nanoélectronique comportant au moins une partie sensible et un canal fluidique (2) défini entre ledit substrat et un capot (6), ledit canal fluidique (2) comportant au moins deux ouvertures pour assurer une circulation dans ledit canal, ladite structure microélectronique et/ou nanoélectronique étant située à l'intérieur du canal fluidique, ledit capot étant assemblé avec le substrat au niveau d'une interface d'assemblage, ledit dispositif comportant des connexions électriques entre ladite structure microélectronique et/ou nanoélectronique et l'extérieur du canal fluidique (2), lesdites connexions électriques (8) étant formées par des vias réalisés à travers le substrat (4) à l'aplomb de ladite structure microélectronique et/ou nanoélectronique et en contact électrique avec ladite structure microélectronique et/ou nanoélectronique.

METHOD FOR REALIZATION OF MICROCAVITIES AND APPLICATION HAS AN ELECTROCHEMICAL SENSOR AS HAS A CHOMATOGRAPHE IN GAS PHASE

Mikrostruktur för vätskeflödessystem och förfarande för tillverkning av ett sådant system

NANOSTRUCTURES CONTAINING CARBON NANOTUBES AND METHODS OF THEIR SYNTHESIS AND USE

The invention relates to novel micro- or nanostructures incorporating nanotubes, wherein nanotubes are synthesized or grown directly in or on components of a micro- or nano-structure. In a particular embodiment, the invention relates to methods of synthesizing or growing nanotubes in a gas chromatography column and their use in portable gas chromatography devices.

CHROMATOGRAPHIC DEVICE AND METHOD OF FABRICATION AND CHROMATOGRAPHIC METHODS

A chromatographic device for use in multi-dimensional GC is described having a gas flow channel means having an inlet and an outlet, and including a first length of tube defining a first stage and a second length of tube defining a second stage; wherein each of the first length of tube defining a first stage and second length of tube defining a second stage is microfabricated in the plane of a planar substrate layer such that each length of tube comprises a bore defining a closed curve in cross section. A GC assembly further comprising modulator, injector and detector and a method of fabrication of device and assembly are described. A method of analysing multi-dimensional GC data is described.

PRE-CONCENTRATOR AND METHOD OF USING THE SAME

A chemical pre-concentrator (30) is provided having a support structure (32), an airflow conduit (34), and a layer of a reactive chemical compound on a surface (44) of the support structure that is useful for collecting and pre-concentrating at least one chemical analyte from a dilute sample. A method of concentrating a gaseous sample is provided that includes exposing the chemical pre-concentrator with a dilute gaseous sample that contains at least one chemical analyte; and forming a conjugate of the at least one chemical analyte. A method of diagnosing a disease state in a mammalian patient is provided using the chemical pre-concentrator.

CHROMATOGRAPHY APPARATUS HAVING DIFFUSION-BONDED AND SURFACE-MODIFIED COMPONENTS

A microfluidic device for separating a sample by chromatography includes diffusion bonded metallic sheets joined together to create a hermetically sealed interface between each adjacent metallic sheet without the introduction of a secondary material. Enclosed within the diffusion bonded sheets is a separation channel accessible by at least one of an inlet or an outlet. The separation channel is packed with micrometer-sized particles serving as a stationary phase in a chromatographic separation. Wetted surfaces of the separation channel include a coating of an organic material at least one monolayer thick.

FLUID CONNECTIONS

Apparatus comprising a micro engineered structure and a capillary or other small bore tube and a method for connecting the tube to the structure. The micro engineered structure is composed of at least one substrate (2) in which fluid flow channels (6) are formed, connecting to an aperture (12) into which the tube (14) is inserted. A sealant material is flowed into the aperture around the tube and then hardened in order to seal the tube within the aperture.

RETAINING MICROFLUIDIC MICROCAVITY AND OTHER MICROFLUIDIC STRUCTURES

A microfluidic device that comprises several microchannel structures in which there are an inlet port, an outlet port an there between a structural unit comprising a fluidic function. The structural unit can be selected amongst units enabling a) retaining of nl-aliquots comprising constituents which has been defined by mixing of aliquots within the microfluidic device (unit A), b)mixing of aliquots of liquids (unit B), c) partition of larger aliquots of liquids into smaller aliquots of liquids into smaller aliquots of liquids and distributing the latter individually and in parallel to different microchannel structure of the same microfluidic device (unit C), d) quick penetration into a microchannel structure of an aliquot of a liquid dispensed to an inlet port of a microchannel structure (unit D), and e) volume definition integrated within a microchannel structure (unit E). In the preferred variants the device is adapted for using centrifugal force possibly combined with capillarity for driving liquid flow within the microchannel structures.

METHODS FOR MODELING, PREDICTING, AND OPTIMIZING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY PARAMETERS

A method for modeling high performance liquid chromatography parameters is disclosed. The method can predict retention times, peak widths, and resolution. The method can also perform a multivariate optimization of a separation over two or more user-adjustable parameters. The method can be applied to isocratic and gradient separations and any combination of isocratic and gradient conditions.

FLUID PARTITIONING IN MULTIPLE MICROCHANNELS

A device (3) and method to generate independent fluid samples (51) for multichannel analysis, preferably in diagnostic cartridges, are disclosed according to the invention. A fluidic device (3), preferably a microfluidic device, has a plurality of fluid channels (35). Fluids are transported in the fluid channels. A cross-over channel (32) has a fluid inlet (33) and a fluid outlet (34). In use of said device (3), a method is performed. According to the method, the sample channels are filled with sample fluid up to a threshold (39). A flush fluid (gas or inert liquid) is then flushed through the sample-filled cross-over channel, replacing the sample fluid with flush fluid. Subsequently the cross-over channels’ inlet and outlet are closed and the sample fluid is pushed further into the channel arrays (30, 31). Alternatively, an appropriate pressure is applied to the fluid in order to push the fluid into said sample channels. The method steps are repeated in an appropriate way if it is ...

Mobile phase gradient generation microfluidic device

The present invention relates to a microfluidic device for separating the components of a fluid sample. A cover plate is arranged over the first surface of a substrate, which, in combination with a microchannel formed in the first surface, defines a separation conduit for separating the components of the fluid sample. An inlet port in fluid communication with the separation conduit allows a mobile phase containing a gradient of a selected mobile-phase component to be introduced from an integrated gradient-generation means to the separation conduit. A method is also provided for separating the components of a fluid sample using a mobile phase containing a gradient of a selected mobile-phase component, wherein the gradient is generated within a small volume of mobile phase.

Integrated monolithic microfabricated electrospray and liquid chromatography system and method

An electrospray device, a liquid chromatography device and an electrospray-liquid chromatography system are disclosed. The electrospray device comprises a substrate defining a channel between an entrance orifice on an injection surface and an exit orifice on an ejection surface, a nozzle defined by a portion recessed from the ejection surface surrounding the exit orifice, and an electrode for application of an electric potential to the substrate to optimize and generate an electrospray; and, optionally, additional electrode(s) to further modify the electrospray. The liquid chromatography device comprises a separation substrate defining an introduction channel between an entrance orifice and a reservoir and a separation channel between the reservoir and an exit orifice, the separation channel being populated with separation posts perpendicular to the fluid flow; a cover substrate bonded to the separation substrate to enclose the reservoir and the separation channel adjacent the cover substrate ...

Block copolymer mask for defining nanometer-scale structures

A nanometer-scale mask includes a periodic array of nanometer-scale structural elements comprising an inorganic oxide.

Liquid chromatograph

A liquid chromatograph comprises an analyzing chip in which a capillary is formed in a substrate and a detector section is disposed downstream of the capillary. The capillary is covered so that an inlet opening end and an outlet opening end of said capillary flow path is opened. A frame member has a sample introduction path, a carrier liquid introduction path and a liquid discharge path. The analyzing chip is movably disposed in the frame member so that the inlet opening end and the outlet opening end of the capillary are selectively in communication with and/or under interruption against said sample introduction path, the carrier liquid introduction path and the liquid discharge path. The liquid chromatograph is very small in size but is easy to handle. Incidentally, there is disclosed also a sample introduction apparatus and method for the chromatograph, and an FET type detector suitable for the chromatograph.

Capillary column chromatography process and system

A microfluidic system is provided for separating components of many fluid samples in a parallel fashion through an array of capillary conduits. The system includes capillary conduits for receiving fluid samples, and for effecting separation of substituents of the sample by passing the sample through a corresponding array of capillary separation conduits containing a solid separation medium. The microfluidic circuit is made of three or more substrate layers and preferably includes etched channels in different layers that are dimensioned relative to one another to provide for retaining the solid composition within the capillary separation conduits. The system also includes an array of detector flow cells in fluid communication with the capillary separation conduits, and an array of high pressure connectors for connecting discrete capillary tubes to the fluid passages in the microfluidic circuit for introducing and removing the fluid samples from the system. The connections for the capillary ...

Microfluidic detection device having reduced dispersion and method for making same

A microfluidic detection device provides reduced dispersion of axial concentration gradients in a flowing sample. The microfluidic detection device includes a cell body and a flow path through the cell body. The flow path has an inlet segment, an outlet segment, and a central segment, which forms a detection cell. The central segment is located between and at an angle with both the inlet segment and the outlet segment. The central segment has a first junction with the inlet segment and a second junction with the outlet segment. The cell body contains two arms that can transmit light to and from the detection cell. At least a portion of a first arm is located in the first junction and at least a portion of a second arm is located in the second junction. The portions of the arms located in the junctions are situated so that fluid entering or exiting the central segment of the flow path flows around the outer surface of one of the portions. By ensuring that the flow velocity is high near the ...

Micromechanical ejection pump for separating small fluid volumes from a flowing sample fluid

A micromechanical ejection pump for extracting small fluid volumes from a flowing sample fluid is provided with a substrate, and a canal for pressurized working fluid. The micromechanical ejection pump also has a canal for ejecting a fluid volume from the sample fluid. The working fluid canal runs into the extension of the sample fluid canal discharging crosswise thereto. The ejection canal connected to the sample fluid canal branches off from the working fluid canal opposite to the sample fluid canal. The working fluid canal and the ejection canal have in particular a substantially similar cross-section of 0.01-0.12 mm2, wherein the ratio of the cross-section of the sample fluid canal to the cross-section of the working fluid canal or the ejection canal is preferably between 0.2 and 0.5, more particularly between 0.3 and 0.4 and most preferably 0.35.

Micro-fluidic device for measuring osmotic second virial coefficients

A micro-fluidic device having a cavity containing a solid support (e.g., a plurality of particles) and a biomolecule immobilized on a surface of the solid support is provided. The micro-fluidic device can be used to monitor interactions between a biomolecule in a sample and the biomolecule immobilized on the solid support. For example, the micro-fluidic device can be used to monitor protein self-interactions wherein the protein in the sample is the same protein as that immobilized on the surface of the solid support. A method of making the micro-fluidic device is also described. The micro-fluidic device can be used for chromatographic applications where sample consumption is crucial.

Microfluidic system

A dual-side field-emission display has one cathode plate and two anode plates. The cathode plate has two opposite surfaces, and each surface has a cathode conductive layer and an electron emission layer formed thereon. The anode plates are disposed at two opposing sides of the cathode plate. Each of the anode plates has an anode conductive layer and a phosphor layer.

LC-MFR-MS-Based Method and Apparatus for Screening a New Drug Candidate

The present invention relates to a method for screening a new drug candidate using a liquid chromatography/microfluidic device/mass spectrometry system, and to a liquid chromatography/microfluidic device/mass spectrometry system. The present invention involves the detection of an interaction between molecules on a real-time basis through adjustment of a microreaction between traces of natural material or synthesized new drug candidates and a target material (protein or cell, etc.), thus developing materials for new drug candidates at a lower cost and with high efficiency, while improving quality of life and reducing medical costs. The present invention can be valuably used in increasing new scientific technology through the convergence of nanotechnology, biotechnology, and analytical chemistry technology.

Fabrication of ultra-shallow channels for microfluidic devices and systems

A method for etching an ultra-shallow channel includes using an etch process that is selective for one material to etch a different material in order to achieve a very precise channel depth in the different material. Channels as shallow as 10 nm can be fabricated in silicon with precision of 5 nm or better using the method. Stepped channels can be fabricated where each segment is a different depth, with the segments being between 10 nm and 1000 nm in depth. The method is applied to create a fluidic channel which includes a channel substrate to which is bonded a lid substrate to confine fluids to the fluidic channels so fabricated.

Fluid transport apparatus and method

A fluid transport apparatus, comprising a transport channel including a fluid inlet; and an evaporator including at least one evaporator channel arranged to receive fluid, each evaporator channel having at least one open fluid outlet operable to evaporate fluid at the at least one fluid outlet so as to cause the flow of fluid thought the transport channel.

Microfluidic devices for liquid chromatography and mass spectrometry

In one aspect, a microfluidic device includes a substrate with a top surface and a raised channel architecture in which at least one channel is formed and defined across a top surface of the substrate and between raised side walls such that a floor of the channel is coplanar with the top surface. The device has a cover positioned over the substrate in alignment with the substrate and including a seal portion that is sealingly received between the raised side walls so as to seal the at least one channel. In addition, the device includes a column packing material disposed within the at least one channel between the raised side walls prior to sealing the at least one channel by merely only inserting the seal portion of the cover within the at least one channel between the raised side walls.

A MEMS Gas Chromatograph and Method of Forming a Separator Column for a MEMS Gas Chromatograph

A micro gas chromatograph includes one or more separator columns formed within a device layer. The separator columns have small channel cross sections and long channel lengths with atomic-smooth channel sidewalls enabling a high channel packaging density, multiple channels positioned on top of each other, and channel segments that are thermally decoupled from the substrates. The micro gas-chromatograph also enables electrostatic and thermal actuators to be positioned in close proximity to the separator columns such that the material passing through the columns is one or more of locally heated, locally cooled, and electrically biased. 1. A method of forming a microelectromechanical system (MEMS) device , comprising:providing a silicon-on-insulator (SOI) wafer including a base layer, a device layer above the base layer, and a buried oxide layer between the base layer and a lower surface of the device layer;etching a plurality of trenches from an upper surface of the device layer to the buried oxide layer, the trenches spaced along a sequential path in the device layer;releasing a portion of the buried oxide layer through the trenches; andannealing the SOI wafer to seal off the trenches at the upper and lower surfaces of the device layer so as to form (i) one or more atomic-smooth, continuous channels within the device layer and (ii) a cavity bounded by the base layer, the buried oxide layer, and the lower surface of the device layer.2. The method of claim 1 , wherein:the one or more atomic-smooth, continuous channels includes a first continuous channel and a second continuous channel, andthe device layer defines (i) a first ingress leading into the first continuous channel and a first egress leading out of first continuous channel and (ii) a second ingress leading into the second continuous channel and a second egress leading out of the second continuous channel.3. The method of claim 2 , wherein:the first continuous channel is disposed substantially along a first ...

Hand-held microfluidic testing device

A hand-held microfluidic testing device is provided. The testing device includes a housing having at least one cartridge receiving port and at least one cartridge for inputting to the cartridge receiving port, and an optical detection system in the housing, where the optical detection system is disposed to analyze at least one sample in the in at least one detection channel of the cartridge. The cartridge includes at least one sample inlet and sample outlet, at least one buffer inlet and buffer outlet, at least one detection material inlet and outlet, at least one calibration standard inlet and calibration standard outlet and a detection window disposed above a detection region of a channel, where the analysis cartridge is surrounded by a protective shell, that holds the analysis chip base, a chip lid, a sealing gasket, and protective film cover.

Characterization of reaction variables

A microscale method for the characterization of one or more reaction variables that influence the formation or dissociation of an affinity complex comprising a ligand and a binder, which have mutual affinity for each other. The method is characterized in comprising the steps of: (i) providing a microfluidic device comprising a microchannel structures that are under a common flow control, each microchannel structure comprising a reaction microactivity; (ii) performing essentially in parallel an experiment in each of two or more of the plurality of microchannel structures, the experiment in these two or more microchannel structures comprising either a) formation of an immobilized form of the complex and retaining under flow conditions said form within the reaction microactivity, or b) dissociating, preferably under flow condition, an immobilized form of the complex which has been included in the microfluidic device provided in step (i), at least one reaction variable varies or is uncharacterized ...

Separation column devices and fabrication methods

Pressure-driven microfluidic separation devices, such as may be used for performing high performance liquid chromatography, are provided. Multiple separation columns may be defined in a single device and packed with stationary phase material retained by porous frits. One or more splitters may be provided to distribute slurry and/or mobile phase among multiple separation columns. In one embodiment, separation devices are substantially planar and fabricated with multiple device layers. Systems and methods employing slurry for packing separation devices are also provided.

GAS SENSOR WITH AN ANALYTE MODULATOR

GAS CHROMATOGRAPH COLUMN WITH CARBON NANOTUBE-BEARING CHANNEL

A MICROFABRICATED DEVICE AND METHOD FOR MULTIPLEXED ELECTROKINETIC FOCUSING OF FLUID STREAMS AND A TRANSPORT CYTOMETRY METHOD USING SAME

Miniaturized device for sample processing and mass spectroscopic detection of liquid phase samples

A miniaturized planar device is described for use in a liquid phase analysis system. The device comprises a separation compartment that is in fluidic communication with a make-up flow channel and a channel compartment that terminates in a on-device mass spectrometer delivery means. The device is formed by microfabrication of microstructures in novel support substrates. The invention herein is used for the analysis of small and/or macromolecular and/or other solutes in the liquid phase.

MICROFLUIDIC DETECTION DEVICE

LIQUID FLOW MANAGEMENT MEANS

СИСТЕМА ПОДАЧИ ЖИДКОСТИ С ТОЧНО ЗАДАННЫМ НЕПРЕРЫВНО РЕГУЛИРУЕМЫМ ДАВЛЕНИЕМ

Изобретение относится к регуляторам давления и может использоваться для отделения функции подачи количества текучей среды от функции регулирования характеристик потока текучей среды. Регулятор характеристик потока текучей среды содержит конфигурацию, которая определяет внутреннюю камеру, канал потока первой текучей среды, канал потока второй текучей среды, гибкую перегородку, расположенную между каналом потока первой текучей среды и каналом потока второй текучей среды, датчик отклонения потока текучей среды, воспринимающий перемещение гибкой перегородки под действием потока первой текучей среды, сигнал отклонения потока текучей среды, генерируемый датчиком отклонения потока текучей среды, принимая во внимание перемещение гибкой перегородки. Также регулятор содержит анализатор сигналов отклонения потока текучей среды, выполненный с возможностью генерирования корректирующего значения второй текучей среды в ответ на сигнал отклонения текучей среды, корректирующий элемент подачи второй текучей ...

СИСТЕМА ПОДАЧИ ЖИДКОСТИ С ТОЧНО ЗАДАННЫМ НЕПРЕРЫВНО РЕГУЛИРУЕМЫМ ДАВЛЕНИЕМ

... 1. Способ регулирования потока текучей среды, включающий этапы, на которых:а) создают источник текучей среды;б) создают поток текучей среды, имеющий характеристики потока текучей среды из источника текучей среды;в) подают поток текучей среды, имеющий упомянутые характеристики потока текучей среды, в регулятор характеристик потока текучей среды;г) измеряют фактический уровень по меньшей мере одной из характеристик потока текучей среды в регуляторе характеристик потока текучей среды;д) сравнивают фактический уровень по меньшей мере одной из характеристик потока текучей среды в регуляторе характеристик потока текучей среды с предварительно заданным уровнем по меньшей мере одной характеристики потока текучей среды;е) подают поток управляющей текучей среды, имеющий управляющие характеристики потока текучей среды, в регулятор характеристик потока текучей среды;ж) обеспечивают контакт потока управляющей текучей среды, имеющего по меньшей мере одну характеристику потока управляющей текучей среды ...

Trennsäule für Chromatographen und Verfahren zur Herstellung

Trennsäule für Chromatographen, die dafür vorgesehen ist, zwischen einem Injektionssystem (1) für eine Probe und einem Detektor (2) angeordnet zu werden, und die mindestens einen Kanal (10) aufweist, der dafür vorgesehen ist, von der Probe durchströmt zu werden, wobei der Kanal (10) eine Oberflächenbeschaffenheit aufweist, die zur Erzeugung unterschiedlicher Strömungsgeschwindigkeiten unterschiedlicher Komponenten der Probe innerhalb der Trennsäule (3) vorgesehen ist, wobei diese Oberflächenbeschaffenheit durch anorganische Nanoröhren ausgebildet ist, dadurch gekennzeichnet, dass die anorganischen Nanoröhren ein Valve-Metall umfassen.

MIKROHERGESTELLTER CHEMISCHER SENSOR AUF DIFFUSIONSBASIS

METHODE ZUR VERBINDUNG EINER KAPILLARE MIT EINER MIKROFLUIDVORRICHTUNG

Micro fluidic structure producing method, involves implementing reactive ion etching on masked hard layer, removing structured photo resist layer, and etching micro fluidic structure in hard masked titanium substrate

The method involves precipitating a hard mask on a titanium substrate, masking the hard mask with a photoresist layer, and structuring the photo resist layer. A reactive ion etching (RIE) is implemented on the masked hard layer to structure a micro fluidic structure on the hard mask. The structured photo resist layer is removed, and the micro fluidic structure is etched in the hard masked titanium substrate under utilization of a deep reactive ion etching (DRIE) e.g. chlorine-based DRIE etching and a chlorine-boron-trichloride-mixture-based DRIE etching.

Verfahren zur Herstellung eines Gaskonverters und entsprechender Gaskonverter

Beschrieben und dargestellt ist ein Verfahren zur Herstellung eines Gaskonverters (1). Die Aufgabe der Erfindung besteht darin, ein Verfahren zur Herstellung eines Gaskonverters vorzuschlagen, das in Erweiterung des Standes der Technik eine Verbesserung darstellt. Die Aufgabe wird bei dem in Rede stehenden Verfahren dadurch gelöst, dass eine Katalysatorschicht (4) auf einer Lage (5) eines Mehrlagenträgerelements (6) aufgebracht wird. Die Erfindung bezieht sich weiterhin auf einen mit dem Verfahren hergestellten Gaskonverter (1).

FLÜSSIGKEITSVERBINDUNGEN

MIKROMECHANISCHE EJEKTIONSPUMPE ZUM HERAUSTRENNEN KLEINSTER FLUIDVOLUMINA AUS EINEM STRÖMENDEN PROBENFLUID

A ring shaped counter electrode to improve beam stability and compound sensitivity on a ceramic tile type microfluidic device

METHOD FOR SPECTRUM MATCHING

A method for matching one spectrum to another, when one spectrum has smoothly varying positional and amplitude instrumental distortions which are superimposed on amplitude differences which represent intersample variations, comprises creating a one-to-one mapping (as shown) between peaks in the two spectra which maximises the correlations between the local structures around the peaks and simultaneously minimises the curvature of the distortions. ...

Chromatograph

Microfluidic apparatus with at least one fluidic channel

Microchemical nanofactories

Embodiments of an apparatus, system, and method for chemical synthesis and/or analysis are disclosed. One embodiment of a disclosed apparatus comprises a laminated, microfluidic structure defining a reactor and a separator. Such apparatuses, or portions thereof, generally have dimensions ranging from about 1 micrometer to about 100 micrometers. To implement synthetic processes, disclosed embodiments of the apparatus generally include at least one unit operation, such as a mixer, a valve, a separator, a detector, and combinations thereof. Individual apparatuses may be coupled both in series and in parallel to form a system for making chemical compounds. An individual apparatus or a system also can be used in combination with known devices and processes.

System for analyzing a gas mixture including at least one chromatography column

A system for analyzing a gas mixture, including at least one chromatography column, a mechanism injecting the mixture into the column, and a mechanism detecting compound(s) forming the gas mixture, the detection mechanism including at least one detector of nanosensor type of an outlet of the column and a detector of nanosensor type in the column, capable of detecting passage of the compounds. It is then possible to determine the velocity of each of the compounds within the system.

Miniature hplc device

A liquid chromatography device comprises one or more liquid reservoirs ( 3 ) for a liquid medium, a sample reservoir for a sample to be analysed and a chromatography column ( 4 ) in fluid communication with the liquid reservoir ( 3 ) and the sample reservoir ( 5 ). The device further comprises a gas reservoir ( 1 ) for containing a volume of gas under pressure to force liquid from the liquid reservoir ( 3 ) through the chromatography column ( 4 ), in use.

Sealed fluidic component comprising a composite material of different paek materials

A sealed fluidic component ( 280 ) for use in a fluidic flow path is made by providing a composite material ( 300 ) comprising a first material ( 305 ) and a second material ( 310 ), wherein the first material ( 305 ) and the second material ( 310 ) are different PAEK materials with the first material ( 305 ) having a lower melting point than the second material ( 310 ). The composite material ( 300 ) is heated in order to provide a sealing by the first material ( 305 ).

MICRO-DEVICE FOR ANALYSIS BY GAS PHASE CHROMATOGRAPHY OFFERING GREAT COMPACTNESS

Device for analysis by gas phase chromatography comprising: 1. Device for analysis by gas phase chromatography comprising:at least one chromatography micro-column,at least one detection module comprising at least one NEMS and/or MEMS type detector arranged in a channel,a direct fluidic connection between an evacuation end of the chromatography micro-column and an admission end of the channel of the detection module,said chromatography micro-column and said detector forming an analysis sub-assembly,a hot face of at least one thermoelectric module being configured to heat the chromatography micro-column formed bythe cold face of the thermoelectric module being configured to cool the detection module,2. Device for analysis according to claim 1 , comprising at least one first and one second thermoelectric module.3. Device for analysis by gas phase chromatography comprising:at least one chromatography micro-column,at least one detection module comprising at least one NEMS and/or MEMS type detector arranged in a channel,a direct fluidic connection between an evacuation end of the chromatography micro-column and an admission end of the channel of the detection module,said chromatography micro-column and said detector forming an analysis sub-assembly,wherein a hot face of at least one first thermoelectric module begin configures to heat the chromatography micro-column,wherein the cold face of a second thermoelectric module being configured to cool the detection module, andwherein a cold face of the first thermoelectric module is in thermal contact with a hot face of the second thermoelectric module.4. Device for analysis according to claim 3 , wherein the cold face of the first thermoelectric module is in direct contact with the hot face of the second thermoelectric module.5. Device for analysis according to claim 3 , wherein a cold face of the first thermoelectric module is in contact with a heat sink claim 3 , a hot face of the second thermoelectric module is in contact ...

Mobile Phase Degassing for Nano-Flow Liquid Chromatography

Systems and methods for degassing liquids in nano-flow liquid applications (). In a chromatography embodiment, a system includes a buffer container, a degasser, a buffer pump, a nano-flow pump, and a separation column. The buffer pump is configured to move a buffer from the buffer container through the degasser and the nano-flow pump is configured to move the buffer from the buffer container or the degasser to the separation column. 1. A nano-flow liquid chromatography system , comprising:a buffer container,a degasser in fluid communication with the buffer container,a buffer pump configured to move a buffer from the buffer container through the degasser,a nano-flow pump in fluid communication with at least one of the buffer container and the degasser; anda separation column in fluid communication with the nano-flow pump, wherein the nano-flow pump is configured to move the buffer from one of the buffer container and the degasser to the separation column.2. The nano-flow liquid chromatography system of claim 1 , wherein the buffer pump is configured to move the buffer from the buffer container claim 1 , through the degasser claim 1 , and back into the buffer container.3. The nano-flow liquid chromatography system of and further comprising a tee connection positioned at an outlet of the degasser claim 1 , wherein the tee connection is configured to direct a first portion of the buffer from the degasser toward the buffer container and a second portion of the buffer from the degasser toward the nano-flow pump.4. The nano-flow liquid chromatography system of and further comprising a tee connection positioned at an outlet of the degasser claim 1 , and a waste container in fluid communication with the degasser claim 1 , wherein the tee connection is configured to direct a first portion of the buffer from the degasser toward the waste container and a second portion of the buffer from the degasser toward the nano-flow pump.5. A nano-flow liquid degassing system claim 1 , ...

METHODS AND SYSTEMS FOR DIAGNOSING DISEASES

The present disclosure provided methods and systems for diagnosing diseases and monitoring their progression and therapeutic responses by detecting a presence or absence, or an increase or decrease, of one or more substances in a sample. 1. A method for detecting a presence of a plurality of biomarkers in a biological sample of a subject , comprising:(a) providing a microfluidic device including at least one fluid channel in fluid communication with at least one emitter having a plurality of nozzles that are operatively coupled to a detector, wherein said fluid channel includes a separation medium that is adapted to separate said plurality of biomarkers into subsets of biomarkers along said fluid channel, and wherein said detector is adapted to generate signals that are indicative of each of said subsets of biomarkers;(b) directing said biological sample having a volume less than or equal to about 50 microliters through said fluid channel to said at least one emitter under conditions that permit said plurality of biomarkers to be separated into said subsets of biomarkers along said fluid channel;(c) directing at least a portion of said biological sample from said plurality of nozzles to said detector, wherein said detector generates signals upon exposure to said at least said portion of said biological sample; and(d) detecting a presence of said subsets of biomarkers based on said signals generated in (c) to detect said presence of said plurality of biomarkers in said biological sample.2. The method of claim 1 , wherein said microfluidic device is part of a disposable chip.3. (canceled)4. The method of claim 1 , wherein said nozzles extend from a base tube having a larger cross-sectional dimension than said nozzles claim 1 , and wherein said base tube is in fluid communication with said fluid channel.5. (canceled)6. The method of claim 4 , wherein said nozzles and said base tube are monolithic.7. The method of claim 4 , wherein said nozzles have a cross-sectional ...

PORTABLE GAS ANALYSIS DEVICE WITH COMPACT MEASUREMENT DEVICE

A portable gas analysis device having a separating column and a detector. The separating column is composed as a multi-capillary unit from parallel individual capillaries and, depending on the length, is bent into a compact shape, preferably even wound into multiple turns. A thermally conductive casing and a thermal stabilizing device are provided for the multi-capillary unit. The thermal stabilizing device comprises a temperature sensor, a heating element and control electronics. The casing protects the sensitive multi-capillary unit from mechanical actions; it acts as a protective space. The temperature-controlled casing also forms a space in which uniform and controlled conditions prevail and which in particular is isolated from the temperature and humidity of the environment, allowing reliable measurements outside a laboratory environment, in the field. This double effect of the casing for the capillaries in conjunction with the compact dimensions forms the true essence of the invention. 1. A portable gas analysis device comprising a measuring unit that comprises:a detector;a separation column embodied as a multi-capillary unit that comprises capillaries arranged in parallel in bent form;a casing for the multi-capillary unit, the casing comprising a material having good thermal conductivity; anda thermal stabilization unit that comprises a temperature sensor and a heating element located on the casing.2. The portable gas analysis device of claim 1 , wherein the multi-capillary unit comprises capillaries wound in multiple turns.3. The portable gas analysis device of claim 1 , wherein multiple heating elements are arranged on the casing such that the multi-capillary unit is temperature-controlled homogeneously.4. The portable gas analysis device of claim 1 , wherein the multi-capillary unit is anchored using a heat-conductive holder in the casing.5. The portable gas analysis device of claim 1 , wherein the casing encloses the multi-capillary unit in a ring shape ...

MICROCHANNEL PHOTOIONIZATION DETECTOR

A microfluidic photoionization detector (PID) may include a substrate and an electrically conductive layer formed on the substrate. The electrically conductive layer may include a microchannel, and a first electrode region and a second electrode region separated from each other by the microchannel, an ohmic contact layer formed on top of the first electrode region and the second electrode region, and a light source formed on the ohmic contact layer for emitting light toward the microchannel. 1. A microfluidic photoionization detector (PID) , comprising:a substrate;an electrically conductive layer formed on the substrate, the electrically conductive layer including a microchannel, wherein the electrically conductive layer further includes a first electrode region and a second electrode region separated from each other by the microchannel;an ohmic contact layer formed on top of the first electrode region and the second electrode region; anda light source formed on the ohmic contact layer for emitting light toward the microchannel.2. The microfluidic PID of claim 1 , wherein the electrically conductive layer is formed of a doped semiconductor material.3. The microfluidic PID of claim 1 , wherein the first electrode region and the second electrode region are formed with a plurality of concave patterns.4. The microfluidic PID of claim 1 , wherein the ohmic contact layer is formed with a plurality of concave patterns.5. The microfluidic PID of claim 1 , wherein the ohmic contact layer may be a layer in which an electrically conductive material is uniformly included.6. The microfluidic PID of claim 1 , wherein the ohmic contact layer may be a multilayer comprising:a first layer formed on top of the first electrode region and the second electrode region; anda second layer formed on top of the first layer.7. The microfluidic PID of claim 1 , further comprising:a light transmitting layer disposed between the ohmic contact layer and the light source.8. The microfluidic PID of ...

DEVICE AND METHODS USING POROUS MEDIA IN FLUIDIC DEVICES

A system that incorporates teachings of the subject disclosure may include, for example, a process that includes obtaining a porous medium comprising a porous material having a first shape and an initial porosity profile. The porous medium is engaged with a cavity in a fluidic device, wherein the cavity is in fluid communication with a channel of the fluidic device. The first shape of the porous material can be adjusted to a second shape resulting in the initial porosity profile being adjusted to a target porosity profile. Such adjustment can be accomplished by the engaging of the porous medium with the cavity, by pre-adjusting a shape of the porous media before insertion into the cavity, or by some combination thereof. Other embodiments are disclosed. 1. An apparatus , comprising:a fluidic device having a fluidic channel and a cavity, the fluidic channel having a channel diameter to accommodate a fluid flow and the cavity having an open end in proximity to a surface of the fluidic device, wherein the cavity has a dimension that is substantially greater than the channel diameter and wherein the cavity is in fluid communication with the fluidic channel; anda pre-formed porous medium comprising a porous material disposed within the cavity, the porous medium having a porosity profile and being in fluid communication with the fluidic channel, wherein the fluid flow is directed through at least a portion of the pre-formed porous medium and wherein the porosity profile comprises an inner region having a first porosity and an outer region having a second porosity that differs from the first porosity, wherein the inner region and the outer region are aligned along a common axial reference location.2. The apparatus of claim 1 , wherein the fluidic device is a microfluidic device.3. The apparatus of claim 1 , wherein the inner region of the porous material has a higher porosity than the outer region of the porous material claim 1 , wherein the inner region is in proximity to ...

Method for producing a functional unit and corresponding functional unit

A method for producing a functional unit with a gas converter ( 1 ) and a flame ionization detector ( 10 ) is produced with the gas converter ( 1 ) and the flame ionization detector ( 10 ) being connected together as parts of a multi-layer ceramic ( 6 ).

GAS CHROMATOGRAPHY COLUMN WITH POLYBUTADIENE COATING

A 3D gas chromatography (GC) column development is possible by assembly of two parts each being substrates formed by gas tight materials. One part may be a silicon substrate with a snake shaped flow channel structure and the other part may be a glass plate. Both are coated with a column packing comprising polubutadiene, which is also able to glue or bond both parts together, thereby sealing the flow channel, thus forming a GC column. The column packaging can be composed in all kinds of polarity from very hydrophobic till very hydrophilic. In this way the column packing can be tuned on resolution for particular molecules which are interesting to detect, e.g. Octane. The invention is advantageous for micro GC columns. 2. The gas chromatography column according to claim 1 , wherein said additional material is selected so as to provide a molecule selectivity to allow detection of Octane in the gas sample.3. The gas chromatography column according to claim 1 , wherein said additional material comprises linoleic acid which is anchored to the polybutadiene.4. The gas chromatography column according to claim 1 , wherein the first and second layers have a thickness of 0.1 μm to 10 μm.5. The gas chromatography column according to claim 1 , wherein the flow channel has a width of 1 μm to 300 μm.6. The gas chromatography column according to claim 1 , wherein the first and second coating materials are identical.7. The gas chromatography column according to claim 1 , wherein the first substrate has a plane surface in which a groove has been formed claim 1 , wherein the groove defines the flow channel claim 1 , and wherein the second substrate has a plane surface arranged to seal the flow channel when bonded to said plane surface of the first substrate.8. The gas chromatography column according to claim 1 , wherein the flow channel forms a curved shape in said plane between the inlet and outlet.9. The gas chromatography column according to claim 1 , wherein the first substrate is ...

CHROMATOGRAPHY APPARATUS HAVING DIFFUSION-BONDED AND SURFACE-MODIFIED COMPONENTS

A microfluidic device for separating a sample by chromatography includes diffusion bonded metallic sheets joined together to create a hermetically sealed interface between each adjacent metallic sheet without the introduction of a secondary material. Enclosed within the diffusion bonded sheets is a separation channel accessible by at least one of an inlet or an outlet. The separation channel is packed with micrometer-sized particles serving as a stationary phase in a chromatographic separation. Wetted surfaces of the separation channel include a coating of an organic material at least one monolayer thick. 1. A diffusion-bonded product manufactured comprising the steps of:supplying two or more substantially compositionally similar metal sheets with each having a flat major surface with no layer thereon to promote bonding; at least one of the two or more metal sheets including at least a portion of a microfluidic channel disposed therein;bringing the flat major surface of each of the two or more metal sheets into a contacting relationship with at least one of the two or more sheets thereby forming an interface and forming and enclosing the microfluidic channel at the interface between such sheets, the microfluidic channel having at least one entrance port and at least one exit port;heating the contacting sheets in a vacuum furnace or an inert-atmosphere furnace to a temperature substantially below melting temperature of such sheets;urging the contacting sheets together under a compressive stress while the sheets are being heated to bond the sheets together by causing grains of the two or more metal sheets to merge across the interface from one sheet to the other sheet;cooling the bonded two or more sheets to about room temperature; andapplying at least an organic coating to the microfluidic channel enclosed between the bonded two or more sheets through at least one of the at least one entrance port or the at least one exit port.2. The diffusion-bonded product of claim ...

Portable water quality instrument

A hand-held microfluidic testing device is provided that includes a housing having a cartridge receiving port, a cartridge for input to the cartridge receiving port having a sample input and a channel, where the channel includes a mixture of Raman-scattering nanoparticles and a calibration solution, where the calibration solution includes chemical compounds capable of interacting with a sample under test input to the cartridge and the Raman-scattering nanoparticles, and an optical detection system in the housing, where the optical detection system is capable of providing an illuminated electric field, where the illuminating electric field is capable of being used for Raman spectroscopy with the Raman-scattering nanoparticles and the calibration solution to analyze the sample under test input to the cartridge.

MICRO GAS CHROMATOGRAPHY SYSTEM

Disclosed is a micro gas chromatography system including a fluid feeder for feeding a fluid composed of a carrier gas and a gas mixture containing an analyte component to the next stage, a micro gas preconcentrator chip configured to concentrate and desorb the analyte component contained in the fluid, a micro gas chromatography chip including a micro separation column for separating the analyte component concentrated and desorbed by the micro gas preconcentrator chip, and a micro sensing unit including a micro thermal conductivity detection sensor configured to detect the analyte component separated by the micro gas chromatography chip. 1. A micro gas chromatography system comprising:a fluid feeder configured to feed a fluid composed of a carrier gas and a gas mixture containing at least one analyte component to a micro gas preconcentrator chip;the micro gas preconcentrator chip configured to concentrate and desorb the analyte component contained in the fluid composed of the carrier gas and the gas mixture;a micro gas chromatography chip into which the fluid containing the analyte component desorbed from the micro gas preconcentrator chip is introduced and from which the analyte component contained in the fluid is separately eluted; anda micro sensing unit including a gas detector configured to detect the analyte component eluted from the micro gas chromatography chip,wherein the micro gas chromatography chip includes a micro separation column having a rectangular, circular, or serpentine micro-channel formed in one surface of a substrate, the micro-channel having a plurality of bumps formed on an inside wall surface thereof, wherein some of the bumps are formed on a first side of the inside wall surface of the micro-channel, the other bumps are formed on a second side that is opposite to the first side, and the bumps formed on the first side and the bumps formed on the second side are alternate with each other.2. The micro gas chromatography system according to ...

PORTABLE WATER QUALITY INSTRUMENT

A hand-held microfluidic testing device is provided that includes a housing having a cartridge receiving port, a cartridge for input to the cartridge receiving port having a sample input and a channel, where the channel includes a mixture of Raman-scattering nanoparticles and a calibration solution, where the calibration solution includes chemical compounds capable of interacting with a sample under test input to the cartridge and the Raman-scattering nanoparticles, and an optical detection system in the housing, where the optical detection system is capable of providing an illuminated electric field, where the illuminating electric field is capable of being used for Raman spectroscopy with the Raman-scattering nanoparticles and the calibration solution to analyze the sample under test input to the cartridge. 1. A portable testing device comprising:a. a housing, wherein said housing comprises a cartridge receiving port;b. a cartridge for input to said cartridge receiving port, wherein said cartridge contains a mixture including a water sample comprising an analyte, Raman-scattering nanoparticles and a calibration solution, wherein said calibration solution comprises a chemical compound capable of interacting with the analyte and the Raman-scattering nanoparticles; andc. an optical detection system in said housing, wherein said optical detection system is capable of providing an illuminated electric field, wherein said illuminating electric field is capable of being used for Raman spectroscopy with said Raman-scattering nanoparticles and said calibration solution to analyze said sample under test input to said cartridge,wherein the chemical compound is selected from the group consisting of thiols, amines, silanes, polymeric particles, metallic particles, crown esters, cysteamine, cystamine, diethylaminethanethiol, mercaptopropionic acid, 1-propanethiol, octanethiol, octyldecanethiol, polystyrene, iron, and silica.2. A portable testing device comprising:a. a housing, ...

MICRO-SEPARATOR HAVING STATIONARY PHASE WITH THREE-DIMENSIONAL NANO-STRUCTURE AND METHOD FOR MANUFACTURING THE SAME

A disclosed micro-separator for gas chromatography includes a base substrate having a trench, a channel column disposed in the trench, and a cover member combined with the base substrate and covering the channel column. The channel column includes a stationary phase having pores ordered and three-dimensionally connected to each other. 1. A micro-separator for gas chromatography comprising:a base substrate having a trench;a channel column disposed in the trench; anda cover member combined with the base substrate and covering the channel column,wherein the channel column includes a stationary phase having pores ordered and three-dimensionally connected to each other.2. The micro-separator for gas chromatography of claim 1 , wherein the base substrate includes at least one selected from the group consisting of silicon claim 1 , glass claim 1 , quartz claim 1 , sapphire and a polymer.3. The micro-separator for gas chromatography of claim 1 , wherein the stationary phase includes at least one selected from the group consisting of a polymer claim 1 , a metal and a ceramic.4. The micro-separator for gas chromatography of claim 1 , wherein the stationary phase is disposed on a bottom surface of the trench and is spaced apart from the cover member to define a gas flow path.5. The micro-separator for gas chromatography of claim 1 , wherein the stationary phase includes a lower stationary phase disposed on a bottom surface of the trench claim 1 , and an upper stationary phase combined with a lower surface of the cover member claim 1 , wherein at least a portion of the upper stationary phase is spaced apart from the lower stationary phase to define a gas flow path between the lower stationary phase and the upper stationary phase.6. The micro-separator for gas chromatography of claim 1 , wherein the stationary phase entirely fills the channel column.7. The micro-separator for gas chromatography of claim 1 , wherein a bottom surface of the trench has a concave shape claim 1 , and ...

Microfluidic Methods and Apparatus for Analysis of Analyte Bearing Fluids

A fluid analyzer for analysis of analyte bearing fluids includes an optical source and an optical transducer defining a beam path of an optical beam; a fluid flow cell with a fluid channel, wherein an interrogation region is defined in which the optical beam interacts with the fluids resulting in transducer output signals; and a controller configured and operative to control operation of the fluid analyzer. In one example the fluid analyzer is controlled to (1) combine a third fluid with the first or second fluid, (2) conduct the first fluid and second fluid through the interrogation region in first and second intervals respectively, (3) measure the transducer output signals during the first and second time intervals, and (4) determine, from the transducer output signals measurement, values of the first and second fluids and an indication of a physical property of the first fluid. 1. A fluid analyzer , comprising:an optical source and an optical transducer defining a beam path of an optical beam;a fluid flow cell with a fluid channel, wherein the beam path defines an interrogation region in the fluid channel in which the optical beam interacts with a fluid bearing an analyte;an electromagnetic fluid modulator for changing a characteristic of the fluid between a first time interval and a second time interval at the interrogation region; anda controller,wherein the optical transducer is configured and operative to sample the optical beam after the optical beam interacts with the fluid in the interrogation region and generates transducer output signals,and wherein the controller is configured and operative to (1) control the fluid modulator, (2) measure the transducer output signals from the optical transducer during the first and second time intervals, and (3) determine from the transducer output signals a measurement value indicative of a physical property of the analyte.2. The fluid analyzer of claim 1 , wherein the electromagnetic fluid modulator is a source of an ...

Reducing Dispersion Due To Vias In Planar Microfluidic Separation Devices

A planar microfluidic chemical separation device includes a separation channel that is located in the plane of the device. The device also includes one or more vias situated perpendicular to the separation channel. The vias extends between the separation channel and an outer surface of the substrate for fluid communication with the separation channel. The vias have cross-sectional areas that are substantially less than a cross-sectional area of a first region of the separation channel to inhibit band-broadening caused by passage of a sample band through the one or more vias.

CHROMATOGRAPHY SYSTEMS AND METHODS USING THEM

Certain embodiments described herein are directed to chromatography systems that include a microfluidic device. The microfluidic device can be fluidically coupled to a switching valve to provide for selective control of fluid flow in the chromatography system. In some examples, the microfluidic device may include a charging chamber, a bypass restrictor or other features that can provide for added control of the fluid flow in the system. Methods of using the devices and methods of calculating lengths and diameters to provide a desired flow rate are also described. 125-. (canceled)26. A laminated microfluidic device comprising an internal microchannel formed from laminating individual layers of the laminated microfluidic device to each other , the laminated microfluidic device comprising a first port , a second port and a third port each fluidically coupled to the internal microchannel , wherein each of the first port , the second port and the third port permits fluid flow into and out of the laminated microfluidic device , wherein the laminated microfluidic device is configured to permit flow of sample into the laminated microfluidic device through the first port and to permit the flow of the sample to a first component fluidically coupled to the second port and to a second component fluidically coupled to the third port depending on a pressure within the laminated microfluidic device.27. The laminated microfluidic device of claim 26 , wherein the internal microchannel comprises a bypass restrictor to restrict fluid flow within the internal microchannel.28. The laminated microfluidic device of claim 26 , wherein the laminated microfluidic device comprises stainless steel layers laminated to each other.29. The laminated microfluidic device of claim 26 , wherein the laminated microfluidic device comprises stainless steel plates laminated to each other.30. The laminated microfluidic device of claim 26 , wherein the first port is configured to fluidically couple to a ...

Variable fluidic restrictor having selective restriction

A variable fluidic restrictor of a liquid chromatography system including a stator body, the stator body include a plurality of fluidic channels located within the stator body, wherein each fluidic channel of the plurality of fluidic channels includes a restrictor element, wherein a flow of a fluid through the variable fluidic restrictor is selectively restricted based on a position of an external element coupled to the stator body is provided. Furthermore, an associated method is also provided.

Microfluidic device

A microfluidic device has a cavity in which a functionalized mesh is arranged in a layered configuration to provide a high surface area. The cavity is housed in a body that is easily configured for sample flow to concentrate the analyte, heat release of the analyte from the mesh, and elution of the released analyte. The microfluidic device can be used in a variety of applications by functionalizing the mesh with a suitable affinity media.

BREATHALYZER

A sensor structure is disclosed comprising at least four planar layers subsuming at least one cavity housed but not contained by overlapping apertures through at least two of the planar layers, wherein the at least one cavity comprises a plurality of chambers, and wherein at least one chamber of the plurality of chambers is configured to be in fluid coupling with at least one other chamber. The plurality of chambers may be defined by overlapping apertures through a plurality of the planar layers. The plurality of chambers may include a Gas Chromatograph (GC) column. The planar layers may be flexible flat glass. The planar layers may be fused together. The layers may be made with apertures through the layers disposed in a desired pattern to define complex structures by the apertures overlapping between abutting layers when the layers are stacked. The planar layers may be configured to admit ultraviolet light. 1. A breathalyzer comprising:a processor;a structure comprising a plurality of abutting planar layers, wherein at least one chamber is defined by individual overlapping apertures through the thickness of, and having an opening central axis substantially perpendicular to, the plane of at least two of the plurality of abutting planar layers;a plurality of sensors operably coupled with the processor, wherein each sensor has a different response characteristic than each of the other sensors of the plurality of sensors; anda memory configured to be operably coupled to the processor, wherein the memory comprises encoded processor executable program instructions and data, wherein the instructions and data, program the processor and configure the breathalyzer, wherein the instructions, when executed by the processor, cause the breathalyzer to perform operations.2. The breathalyzer of claim 1 , wherein the plurality of sensors are disposed in a plurality of respective chambers claim 1 , wherein each individual sensor is disposed in one chamber claim 1 , wherein each ...

System and method of preconcentrating analytes in a microfluidic device

A method and system for preconcentrating analytes at a microvalve in a microfluidic device is disclosed. The system includes a sample channel loaded with a sample solution. The sample channel includes a semi-permeable membrane microvalve. An electric potential is applied at or across the microvalve to preconcentrate the sample solution when the microvalve is closed. The method includes pretreatments of the device or valve for preconcentration of the analytes. For preconcentration of anionic analytes, the device is baked. For preconcentration of the cationic analytes, the surface of the membrane microvalve is coated with a polycationic coating, and the device is baked.

Micro-Machined Frit and Flow Distribution Devices for Liquid Chromatography

A micro-machined frit is provided for use in a chromatography column, having a substrate with a thickness, and holes extending through the thickness and providing fluid communication through the substrate. A micro-machined flow distributor is provided for use in a chromatography column having a substrate, holes extending through the substrate, and channels in fluid communication with the holes. A micro-machined integrated frit and flow distributor device is also provided having a substrate with a thickness, holes extending through the thickness and providing fluid communication therethrough, and channels in fluid communication with at least one of the holes. A chromatography column is provided having a tube, an extraction medium contained therein, and a micro-machined frit positioned proximate an end of the tube. The column can include a micro-machined flow distributor positioned between the frit and the end of the tube. 120-. (canceled)21. A chromatography column comprising:a tube having an inlet end and an opposed outlet end;an extraction medium contained within said tube, said extraction medium comprising particles having an average dimension;at least one micro-machined frit positioned proximate one of said inlet end and said outlet end of said tube, said at least one frit comprising a first substrate having a first surface, an oppositely disposed second surface, and a thickness, said first substrate defining a plurality of first holes extending through said thickness, each of said first holes having a first end positioned on said first surface and an opposed second end positioned on said second surface, wherein for each of said holes, said first end is aligned with said second end, and wherein said holes provide fluid communication therethrough said first substrate; anda support lattice positioned on said first surface, wherein said support lattice defines a plurality of openings, wherein each of said openings is in fluid communication with at least one of said ...

SIMPLE SINGLE-STEP POROUS POLYMER MONOLITH FOR DNA EXTRACTION

A method and microfluidic device with a porous polymer monolith in a channel of the device with capture affinity element (such as an oligonucleotide complementary to a DNA target from the KPC antibiotic resistance gene) on the monolith surface. 1. A method for creating a monolith in a void space of a microfluidic device , comprising:filling the internal void spaces of the monolith with a polymerization mixture comprising one or more photopolymerizable monomers, a photo initiator, and a capture affinity element;masking microfluidic device to prevent light from reaching the internal void spaces with the exception of a gap to allow light to reach a preselected portion of the void spaces;exposing the microfluidic device to light to polymerize polymerization solution in the preselected portion to form in the preselected portion a porous polymer monolith with capture affinity element on a polymer monolith surface, and with the polymer monolith anchored on a device surface within the preselected portion;rinsing unpolymerized polymerization solution from the internal void spaces.2. The method of wherein the capture affinity element is a capture oligonucleotide.3. The method of wherein the polymerizable mixture comprises one or more photopolymerizable acrylate monomers.4. The method of wherein the microfluidic device is exposed to ultra-violet light.5. The method of wherein the filling claim 1 , masking claim 1 , exposing claim 1 , and rinsing steps are conducted once in a single step.6. The method of wherein the acrylate monomers are one or more from the group of PEGDA and EDMA.7. The method of wherein the capture oligonucleotide is complementary to a target DNA sequence.8. The method of wherein the capture oligonucleotide is complementary to a target from a gene from a microorganism.9. The method of wherein the capture oligonucleotide is complementary to a target from a gene from DNA of a bacterium.10. The method of wherein the capture oligonucleotide is complementary to a ...

METHODS AND SYSTEMS FOR DIAGNOSING DISEASES

The present disclosure provided methods and systems for diagnosing diseases and monitoring their progression and therapeutic responses by detecting a presence or absence, or an increase or decrease, of one or more substances in a sample. 1100.-. (canceled)101. A microfluidic device , comprising:a fluid channel comprising an enrichment region and a separation region downstream of said enrichment region; andan emitter in fluid communication with said fluid channel,wherein said enrichment region is configured to (i) receive a solution comprising a plurality of biomarkers and (ii) concentrate said plurality of biomarkers to yield a concentrated solution comprising said plurality of biomarkers, andwherein said separation region is configured to (i) receive said concentrated solution comprising said plurality of biomarkers and (ii) separate said plurality of biomarkers.102. The microfluidic device of claim 101 , wherein said microfluidic device is monolithic.103. The microfluidic device of claim 101 , wherein said microfluidic device comprises a first substrate adjacent to a second substrate claim 101 , and wherein said emitter is disposed between said first substrate and said second substrate.104. The microfluidic device of claim 103 , wherein said first substrate or said second substrate comprises a semiconductor.105. The microfluidic device of claim 103 , wherein said fluid channel is disposed between said first substrate and said second substrate.106. The microfluidic device of claim 101 , wherein said enrichment region has a different dimension than said separation region.107. The microfluidic device of claim 101 , wherein said emitter comprises a nozzle.108. The microfluidic device of claim 107 , further comprising a base tube in fluid communication with said fluid channel claim 107 , wherein said nozzle extends from said base tube having a larger cross-sectional dimension than said nozzle.109. The microfluidic device of claim 107 , wherein said nozzle has a cross- ...

CHROMATOGRAPHY SYSTEMS AND METHODS USING THEM

Certain embodiments described herein are directed to chromatography systems that include a microfluidic device. The microfluidic device can be fluidically coupled to a switching valve to provide for selective control of fluid flow in the chromatography system. In some examples, the microfluidic device may include a charging chamber, a bypass restrictor or other features that can provide for added control of the fluid flow in the system. Methods of using the devices and methods of calculating lengths and diameters to provide a desired flow rate are also described.

Progressive Cellular Architecture For Microfabricated Gas Chromatograph

A progressive cellular architectures has been presented for vapor-phase chemical analyzers. The progressive cellular architecture consists of a series of heterogeneous micro-gas chromatography cells. Each individual cell targets vapor species within a specific volatility range by using a unique combination of a preconcentrator and a separation column. The cells are connected progressively in series to cover a broad range of volatile analyte chemical vapors. Valves may inadvertently absorb or adsorb and subsequently release target chemical analyte molecules, thereby interfering with quantitative analysis. Therefore, the inlet to the cells is configured without a valve. 1. A gas chromatography architecture , comprising:two or more preconcentrators coupled together in series, the two or more preconcentrators having an inlet configured without a valve therein to receive an analyte and an outlet, each preconcentrator comprising a chamber through which the analyte passes and sorbent material within the chamber, wherein surface area of sorbent material residing in a given preconcentrator increases from the inlet to the outlet across the two or more preconcentrators;a sampling pump fluidly coupled to the outlet of the two or more preconcentrators;a sampling valve interposed between the outlet of the two or more preconcentrators and the sampling pump;a separation pump;a separation column associated with each preconcentrator of the two or more preconcentrators, each separation column is disposed in a separation path fluidly coupled between an inlet of the corresponding preconcentrator and the separation pump;a separation valve disposed in each of the separation paths between the corresponding preconcentrator and the separation pump; anda controller operably coupled to the sampling valve and each of the separation valves.2. The gas chromatography architecture of further comprises a flow restrictor disposed in the inlet of the two or more preconcentrators.3. The gas ...

PORTABLE WATER QUALITY INSTRUMENT

A hand-held microfluidic testing device is provided that includes a housing having a cartridge receiving port, a cartridge for input to the cartridge receiving port having a sample input and a channel, where the channel includes a mixture of Raman-scattering nanoparticles and a calibration solution, where the calibration solution includes chemical compounds capable of interacting with a sample under test input to the cartridge and the Raman-scattering nanoparticles, and an optical detection system in the housing, where the optical detection system is capable of providing an illuminated electric field, where the illuminating electric field is capable of being used for Raman spectroscopy with the Raman-scattering nanoparticles and the calibration solution to analyze the sample under test input to the cartridge. 1. A hand-held microfluidic testing device comprising:a. a housing, wherein said housing comprises a cartridge receiving port;b. a cartridge for input to said cartridge receiving port, wherein said cartridge comprises a sample input and a channel, wherein said channel comprises a mixture of Raman-scattering nanoparticles and a calibration solution, wherein said calibration solution comprises chemical compounds capable of interacting with a sample under test input to said cartridge and said Raman-scattering nanoparticles; andc. an optical detection system in said housing, wherein said optical detection system is capable of providing an illuminated electric field, wherein said illuminating electric field is capable of being used for Raman spectroscopy with said Raman-scattering nanoparticles and said calibration solution to analyze said sample under test input to said cartridge.2. The hand-held microfluidic testing device of claim 1 , wherein said chemical compounds are selected from the group consisting of thiols claim 1 , amines claim 1 , silanes claim 1 , polymeric particles claim 1 , metallic particles claim 1 , crown esters claim 1 , cysteamine claim 1 , ...

DEVICE AND METHOD FOR EXTRACTION OF COMPOUNDS WITH AROMATIC CYCLES CONTAINED IN A LIQUID SAMPLE

A device for extraction of analytes with aromatic cycles, preferably analytes with aromatic cycles for which the octanol-water partition coefficient is more than 10, the analytes being contained in a liquid phase, the extraction device including a support and an adsorption layer at least partially covering the support, the adsorption layer being porous SiOxCyHz. 1. The extraction device for the extraction of analytes with aromatic cycles , said analytes being contained in a liquid phase , said extraction device comprising a support and an adsorption layer being porous SiOxCyHz , said adsorption layer at least partially covering said support , the extraction device being configured to put into contact the liquid phase and the adsorption layer.2. The extraction device according to claim 1 , in which the octanol-water partition coefficient of analytes of interest with aromatic cycles is more than 10 claim 1 , and advantageously between 10and 10.3. The extraction device according to claim 1 , wherein x is between 1 and 2 and preferably between 1.4 and 1.8; y is between 0.8 and 3 and preferably between 1 and 2.5 and z is between 2.5 and 4.5 and preferably between 3 and 4.1.4. The extraction device according to claim 1 , wherein the thickness of the adsorption layer is between 50 nm and 2000 nm claim 1 , and preferably between 50 nm and 1000 nm.5. The extraction device according to claim 1 , wherein the porosity of the adsorption layer is between 3% and 60% claim 1 , and preferably between 10% and 40% and the radius of pores is between 1 nm and 5 nm.6. The extraction device according to claim 1 , wherein the support is composed of at least the walls of an extraction chamber claim 1 , said extraction chamber comprising at least one liquid phase supply orifice and at least one liquid phase outlet orifice claim 1 , said supply and outlet orifices being arranged such that the liquid phase comes into contact with the adsorption layer and flows from the supply orifice towards ...

INTEGRATED FLUIDIC SYSTEM FOR GAS CHROMATOGRAPHY

A method is presented for fabricating a fluidic system for a gas chromatograph. The method includes: microfabricating a portion of a fluidic system of a gas chromatograph on a substrate using a first mask; microfabricating a portion of the fluidic system of the gas chromatograph using a second mask; and microfabricating a portion of the fluidic system of the gas chromatograph using a third mask, such that the first mask, the second mask and the third mask are different from each other and the microfabricating of the fluidic system of the gas chromatograph is completed using only the first, second and third masks. A gas chromatograph wherein a microfabricated Knudsen pump is arranged to operate in a first direction to draw carrier gas into a preconcentrator and in a second direction to draw gas out of the preconcentrator. 1. A method for microfabricating a fluidic system for a gas chromatograph having at least three components , comprising:microfabricating, using a first mask, a portion of a fluidic system of a gas chromatograph on a substrate;microfabricating, using a second mask, the same or a different portion of the fluidic system of the gas chromatograph; andmicrofabricating, using a third mask, the same or a different portion of the fluidic system of the gas chromatograph, wherein the first mask, the second mask and the third mask are different from each other and the microfabricating of the fluidic system of the gas chromatograph is completed using only the first, second and third masks.2. The method of further comprisesmicrofabricating three components of the gas chromatograph on separate portions of the substrate using only the first, second and third masks;dicing the substrate into multiple dies, each die having a different sub-assembly for one of the three components disposed on each die; andstacking the multiple dies to form the gas chromatograph.3. The method of wherein microfabricating is further defined as one of sandblasting claim 1 , plasma etching ...

CHROMATOGRAPHY SYSTEMS AND METHODS USING THEM

Certain embodiments described herein are directed to chromatography systems that include a microfluidic device. The microfluidic device can be fluidically coupled to a switching valve to provide for selective control of fluid flow in the chromatography system. In some examples, the microfluidic device may include a charging chamber, a bypass restrictor or other features that can provide for added control of the fluid flow in the system. Methods of using the devices and methods of calculating lengths and diameters to provide a desired flow rate are also described. 125-. (canceled)26. A system for modulating flow of a fluid in a chromatography system , the system comprising:a microfluidic device fluidically coupled to the switching valve, in which the microfluidic device comprises a first charging chamber and a second charging chamber;a detector fluidically coupled to a first outlet of the microfluidic device;a switching valve fluidically coupled to the microfluidic device, the switching valve configured to actuate between a first position and a second position, wherein the first position is configured to permit fluid flow from a modulating gas source to the first charging chamber of the microfluidic device to provide column effluent from the first charging chamber to the detector and wherein second position is configured to permit fluid flow from the modulating gas source to the second charging chamber of the microfluidic device to provide column effluent from the second charging chamber to the detector fluidically coupled to the microfluidic device.27. The system of claim 26 , in which the switching valve is a 3-way solenoid valve that is actuated at a frequency of about 10 Hz to about 100 Hz.28. The system of claim 26 , further comprising a restrictor between the outlet of the microfluidic device and the detector claim 26 , in which the restrictor is configured to balance pressure in system.30. The system of claim 26 , further comprising a second detector ...

PORTABLE WATER QUALITY INSTRUMENT

A hand-held microfluidic testing device is provided that includes a housing having a cartridge receiving port, a cartridge for input to the cartridge receiving port having a sample input and a channel, where the channel includes a mixture of Raman-scattering nanoparticles and a calibration solution, where the calibration solution includes chemical compounds capable of interacting with a sample under test input to the cartridge and the Raman-scattering nanoparticles, and an optical detection system in the housing, where the optical detection system is capable of providing an illuminated electric field, where the illuminating electric field is capable of being used for Raman spectroscopy with the Raman-scattering nanoparticles and the calibration solution to analyze the sample under test input to the cartridge. 1. A portable testing device comprising ,a portable testing device housing, wherein said portable testing device housing comprises a cartridge receiving port;a cartridge for input to said cartridge receiving port, wherein said cartridge contains a mixture including a water sample comprising selenate, metallic Raman-scattering nanoparticles, and a selenate isotope or a sulfate isotope; andan optical detection system in said portable testing device housing, wherein said optical detection system is configured to provide an illuminating electric field, wherein said illuminating electric field is configured to produce Raman spectra of the selenate and isotope simultaneously while the cartridge is input to said cartridge receiving port to allow for a ratiometric analysis of the simultaneously produced selenate and isotope spectra to quantify the selenate in the water sample.2. The portable testing device of wherein said mixture further comprises an amine or thiol.3. The portable testing device of wherein said mixture further comprises an amine.4. A method for determining the concentration of selenate in a water sample comprising the steps of claim 1 ,adding metallic ...

Column Having a Compression-Limited Assembly

A chromatography column has a column assembly that further includes a column jacket tube and a column liner tube. The column assembly is configured with end fittings that have compression limiting features and are able to withstand very high pressure, such as used in UHPLC, in conjunction with flange portions of the column liner tube that seal the column assembly. The column liner tube may be biocompatible and may have a smooth consistent inner diameter of less than 2 mm, and an inner diameter of 1 mm in particular embodiments. The end fittings for the microtube assembly may ensure durability and reliability by preventing over- or under-tightening. 2. The column assembly of claim 1 , wherein claim 1 , when the threaded tube portion threadingly engages with the retainer body claim 1 , the shoulder is configured for detention contact with the frit retainer to limit a compression force applied by the retainer body to the column liner tube.3. The column assembly of claim 2 , wherein the compression force is specified to provide a high pressure fluidic seal by compressing the flange portion against the first cavity.4. The column assembly of claim 3 , wherein the high pressure fluidic seal is enabled to seal the column passageway up to 280 claim 3 ,000 kPa.5. The column assembly of claim 2 , wherein the shoulder in detention contact with the frit retainer forms a secondary seal in addition to the high pressure seal.6. The column assembly of claim 1 , further comprising:a third cavity disposed within the first cavity, the third cavity configured to receive and hold a frit in fluid communication with the column passageway at the flange portion.7. The column assembly of claim 6 , wherein the frit is a dimpled frit having an increased inlet surface area.8. The column assembly of claim 7 , wherein the dimpled frit comprises a porous metal.9. The column assembly of claim 7 , wherein the porous metal is a metal foam.10. The column assembly of claim 1 , further comprising the ...

INTEGRATED HIGH THROUGHPUT SYSTEM FOR THE ANALYSIS OF BIOMOLECULES

Described is an affinity microcolumn comprising a high surface area material, which has high flow properties and a low dead volume, contained within a housing and having affinity reagents bound to the surface of the high surface area material that are either activated or activatable. The affinity reagents bound to the surface of the affinity microcolumn further comprise affinity receptors for the integration into high throughput analysis of biomolecules. 1. An affinity microcolumn comprising a polymer contained within a housing and affinity reagents bound to the polymer , wherein the affinity reagents are either activated or activatable.2. The affinity microcolumn of wherein the polymer is formed by molding.3. The affinity microcolumn of wherein the polymer is exposed to at least one of chemical etching and electro etching.4. The affinity microcolumn of wherein the affinity reagents that are bound to the polymer further comprise affinity receptors bound to the affinity reagents.5. The affinity microcolumn of further comprising a tethering molecule that is activated or activatible and binds the affinity receptors to the affinity reagents.6. The affinity microcolumn of further comprising an amplification media that is activated or activatible and is interposed between the affinity reagents and the affinity receptors claim 4 , where the amplification media allows a high density of affinity receptors to be bound to the affinity reagents than in the absence of the amplification media.7. The affinity microcolumn of further comprising an amplification media interposed between the affinity reagents and the affinity receptors claim 4 , where the amplification media allows better access by an analyte to the affinity receptors than in the absence of the amplification media.8. The affinity microcolumn of wherein the amplification media comprises at least one of a biological polymer claim 6 , a non-biological organic polymer claim 6 , and an inorganic polymer.9. The affinity ...

CHROMATOGRAPHY APPARATUS AND METHODS USING MULTIPLE MICROFLUIDIC SUBSTRATES

An apparatus for chemical separations includes a first substantially rigid microfluidic substrate defining a first fluidic port; a second substantially rigid microfluidic substrate defining second fluidic port; and a coupler disposed between the first and second substrates, the coupler defining a fluidic path in fluidic alignment with the ports of the first and second substrates. The coupler includes a material that is deformable relative to a material of the first substrate and a material of the second substrate. The substrates are clamped together to compress the coupler between the substrates and form a fluid-tight seal. 1. An apparatus for chemical separations , comprising:a first substantially rigid microfluidic substrate comprising a plurality of layers, a first channel formed between the layers, and a first fluidic port in fluid communication with the first channel;a second substantially rigid microfluidic substrate comprising a plurality of layers, a second channel formed between the layers, and a second fluidic port in fluid communication with the second channel,the layers of the first substantially rigid microfluidic substrate comprising a different material from the layers of the second substantially rigid microfluidic substrate; anda coupler disposed between the first and second substrates, the coupler defining a fluidic path in fluidic alignment with the ports of the first and second substrates.2. The apparatus of claim 1 , wherein at least one of the first channel and the second channel defines a separation column in fluidic communication with the respective fluidic port.3. The apparatus of claim 1 , wherein at least one of the first channel and the second channel defines a trap column in fluidic communication with the respective fluidic port.4. The apparatus of claim 1 , further comprising an alignment fitting attached to the first substrate claim 1 , to position the fluidic path of the coupler in fluidic alignment with the first fluidic port.5. The ...

Characterization of reaction variables

A microscale method for the characterization of one or more reaction variables that influence the formation or dissociation of an affinity complex comprising a ligand and a binder, which have mutual affinity for each other. The method is characterized in comprising the steps of: (i) providing a microfluidic device comprising a microchannel structures that are under a common flow control, each microchannel structure comprising a reaction microactivity; (ii) performing essentially in parallel an experiment in each of two or more of the plurality of microchannel structures, the experiment in these two or more microchannel structures comprising either a) formation of an immobilized form of the complex and retaining under flow conditions said form within the reaction microactivity, or b) dissociating, preferably under flow condition, an immobilized form of the complex which has been included in the microfluidic device provided in step (i), at least one reaction variable varies or is uncharacterized for said two or more microchannel structures while the remaining reaction variables are kept essentially constant; (iii) measuring the presentation of the complex in said reaction microactivity in said two or more microchannel structures; and (iv) characterizing said one or more reaction variables based on the values for presentation obtained in step (iii).

Hydrophilic Substrate, Measuring Instrument, Device, and Hydrophilicity Retention Method

The invention provides a technique for retaining hydrophilicity of a surface of a substrate over a long period of time with a simple maintenance without changing the structure and characteristics of the substrate. A component includes a hydrophilic component having a hydrophilic surface, a protective layer that is formed on the surface and contains a soluble sub stance.

Detection System and Method with Nanostructure Flow Cell

A system and method for determining concentration of a constituent of a sample fluid includes a flow cell with a light source emitting incident light to a proximal end thereof. Media disposed within the flow cell supports nanostructures that are substantially transparent in at least a portion of the incident light spectrum. The nanostructures adsorb or absorb the constituent to attain a concentration that is a multiple of the concentration of the constituent in the sample fluid. A sensor detects transmitted light exiting from the media, and generates outputs corresponding to a spectrum of the transmitted light. A processor captures the sensor outputs and compares the incident light spectrum to the transmitted light spectrum to generate an absorbance spectrum. The absorbance spectrum is used to calculate the concentration in the nanostructures, which is then used with the predetermined multiple to calculate the sample concentration. 1. A system for optically determining concentration of a constituent of a sample fluid in real time , the system comprising:a flow cell configured to convey the sample fluid therethrough from a proximal end to a distal end;a light source disposed in optical communication with the proximal end of the flow cell, the light source configured to emit incident light of an incident light spectrum into the flow cell;media disposed within the flow cell;nanostructures supported by the media, the nanostructures being substantially transparent in at least a portion of the incident light spectrum, the nanostructures configured to adsorb or absorb the constituent to attain a nanostructures concentration that is a predetermined multiple of a sample concentration of the constituent in the sample fluid passing through the flow cell;at least one sensor disposed at the distal end of the flow cell, the sensor configured to detect transmitted light exiting from the media, and to generate one or more outputs corresponding to a transmitted light spectrum of the ...

Electrospray emitter assemblies for microfluidic chromatography apparatus

An apparatus for chemical separations includes a microfluidic substrate having an outlet aperture for outputting an eluent of a sample. An emitter assembly includes having a deformable end portion, an inlet near the deformable end portion to receive the sample eluent from the microfluidic substrate, and an electrically conductive outlet portion to emit a spray of the sample eluent. A force-applying unit applies a force to the emitter assembly that urges the deformable end portion into contact with the microfluidic substrate. The deformable end portion is more elastic than the microfluidic substrate so that the contact between the microfluidic substrate and the deformable end portion produces a substantially fluid-tight seal between the outlet aperture of the microfluidic substrate and the inlet of the emitter assembly.

Method for cleaning microfluidic device and cleaning liquid

The present invention provides a cleaning method by which the performance of a microchip can be recovered and a cleaning liquid. A method for cleaning a microfluidic device comprising: cleaning a channel that is formed in the microfluidic device, has a surface having a polymer coating, and has been brought into contact with a sample containing nucleic acid and/or protein, by bringing the channel into contact with a cleaning liquid comprised only of an organic solvent having solubility in at least the same volume of water at 25° C., or a cleaning liquid containing 50 vol % or more of the organic solvent in a buffer solution. The method wherein the buffer solution has a pH of 8 to 10. The method wherein the buffer solution further contains 3 to 8M of a protein denaturant. The method wherein the buffer solution has a pH of 2 to 4.

DERIVATIZATION REACTION GAS CHROMATOGRAPHIC CHIP

Provided is a derivatization reaction gas chromatographic chip including: an analysis solution inlet allowing an analysis solution containing an analysis target to be introduced therethrough; a derivative inlet allowing a reaction solution containing a derivative chemically reacting with the analysis target to be introduced therethrough; a guide channel connecting one end of a first micro-channel to each of the analysis solution inlet and the derivative inlet; the first micro-channel in which a fluid flows and the analysis target in the fluid is vaporized; a first outlet connected to the other end of the first micro-channel; a gas phase inlet communicating with the first outlet; a second micro-channel having one end connected to the gas phase inlet and having a stationary phase formed therein; and a second outlet connected to the other end of the second micro-channel. 1. A derivatization reaction gas chromatographic chip comprising:an analysis solution inlet allowing an analysis solution containing an analysis target to be introduced therethrough;a derivative inlet allowing a reaction solution containing a derivative chemically reacting with the analysis target to be introduced therethrough;a guide channel connecting one end of a first micro-channel to each of the analysis solution inlet and the derivative inlet;the first micro-channel in which a fluid introduced through the analysis solution inlet and the derivative inlet flows and the analysis target in the fluid reacting with the derivative is vaporized;a first outlet connected to the other end of the first micro-channel; a gas phase inlet communicating with the first outlet;a second micro-channel having one end connected to the gas phase inlet and having a stationary phase formed therein; anda second outlet connected to the other end of the second micro-channel,wherein the analysis target contained in the analysis solution is vaporized and the vaporized analysis target is separated by the stationary phase by the ...

Fluidic devices and methods using them

Certain embodiments described herein are directed to chromatography systems that include a microfluidic device configured to provide three-way switching or switching between three or more inputs or outputs. The microfluidic device can be fluidically coupled to one or more switching valves to provide for selective control of fluid flow in the chromatography system.

Analytical Tool and Analytical System

An analytical tool for use in analysis of a sample is provided. The analytical tool includes a first unit and a second unit. The first unit has an analysis portion where analysis is performed. The second unit, configured to be coupled with the first unit, has a liquid reservoir for confining a particular liquid used for the analysis of the sample. When the first unit and the second unit are coupled, a fluid passage for conducting the particular liquid from the liquid reservoir into the first unit is formed by only a part of the first unit, or only a part of the second unit, or a combination of respective parts of the first unit and the second unit. 110.-. (canceled)11. An analytical system comprising:an analytical tool that is formed in a plate shape, and that includes a conductor for causing electrophoresis of a sample;an analytical device to which the analytical tool mounts, and that analyzes a component of the sample electrophoresed at the analytical tool; andan electrode that is provided with the analytical device, and that electrically contacts with the conductor toward a width-direction side surface of the analytical tool to cause the electrophoresis of the sample by applying a voltage.12. An analytical system according to claim 11 , wherein the electrode electrically contacts with the conductor toward the width-direction side surface of the analytical tool claim 11 , in case of a state in which the analytical tool mounts to the analytical device.13. An analytical system according to claim 12 , wherein the analytical tool includes an electrode-receiving recess that is recessed from the width-direction side surface toward an inside of the analytical tool claim 12 , and that houses a portion of the conductor in a longitudinal direction claim 12 , andthe electrode is inserted into the electrode-receiving recess to electrically contact with the conductor.14. An analytical system according to claim 11 , wherein the analytical tool includes an analysis portion that ...

Chip-Scale Gas Chromatography

A miniaturized gas chromatography system integrated on single chip comprising a sample injection unit, a separation column having an inlet, an exit and an interior surface, at least one detector located at the separation column exit and the sample injection unit having a T-shaped configuration. The column may be coated with room temperature ionic liquids, with and without an intermediate layer between the room temperature ionic liquid and the silicon surface. 1. A miniaturized gas chromatography system integrated on single chip comprising:a sample injection unit;a separation column having an inlet, an exit and an interior surface;at least one detector located at the separation column exit; andsaid sample injection unit having a T-shaped configuration.2. The miniaturized gas chromatography system of wherein the system reduces the need for heated interconnect lines between individual components by reducing the footprint claim 1 , cost and power budget for the operation of the system.3. The miniaturized gas chromatography system of wherein the system reduces band-broadening of compounds having high molecular weight thus improving the performance of the system.4. The miniaturized gas chromatography system of wherein said interior surface of said separation column is coated with a room temperature ionic liquid film.5. The miniaturized gas chromatography system of further including a plurality of separation columns each having an interior surface claim 1 , said interior surfaces coated with one or more room temperature ionic liquid films.6. The miniaturized gas chromatography system of wherein said interior surface of said separation column has a silicon surface claim 1 , said silicon surface coated with an alumina surface claim 1 , said alumina surface coated with a one or more room temperature ionic liquid films.7. The miniaturized gas chromatography system of wherein said interior surface of said separation column has a silicon surface claim 1 , said silicon surface ...

3-d glass printable hand-held gas chromatograph for biomedical and environmental applications

A sensor structure is disclosed comprising at least four planar layers subsuming at least one cavity housed but not contained by overlapping apertures through at least two of the planar layers, wherein the at least one cavity comprises a plurality of chambers, and wherein at least one chamber of the plurality of chambers is configured to be in fluid coupling with at least one other chamber. The plurality of chambers may be defined by overlapping apertures through a plurality of the planar layers. The plurality of chambers may include a Gas Chromatograph (GC) column. The planar layers may be flexible flat glass. The planar layers may be fused together. The layers may be made with apertures through the layers disposed in a desired pattern to define complex structures by the apertures overlapping between abutting layers when the layers are stacked. The planar layers may be configured to admit ultraviolet light.

USE OF GLYCAN AS BIOMARKERS FOR AUTOIMMUNE DISEASES

The present invention discloses a method of determining the presence of autoimmune disease with the use of glycan biomarkers. A method of improving the detection sensitivity of trace glycans from a mixture of glycans and a microfluidic chip therefor are also disclosed. 19-. (canceled)10. A microfluidic chip for enriching trace glycan from a mixture of glycans comprising an enrichment column that attaches to an analytical column , a first section that includes porous graphitized carbon;', 'a second section that connects to said first section and includes titanium dioxide; and', 'a third section that connects to said second section and includes porous graphitized carbon;, 'wherein said enrichment column enriches acidic glycans and includes'}wherein said analytical column includes porous graphitized carbon;wherein said first section and said third section of said enrichment column perform pre-enrichment of glycans to remove non-glycan constituents; said second section of said enrichment column performs enrichment of acidic glycans; and said analytical column performs chromatographic separation of glycans, such that detection sensitivity of low-abundance trace glycans is highly enhanced when a mixture of glycans runs through the microfluidic chip.11. The microfluidic chip of wherein said trace glycan is acidic glycan.12. The microfluidic chip of wherein said trace glycan is sulfated glycan.13. The microfluidic chip of wherein said trace glycan comprises a structure set forth in .14. A method of improving the detection sensitivity of trace glycan from a mixture of glycans comprising:a) loading a sample of said mixture of glycans onto an enrichment column;b) running said neutral glycans from step (a) onto an analytical column that performs chromatographic separation of glycans while said acidic glycans are retained on said enrichment column and 'wherein said enrichment column enriches acidic glycans while said analytical column performs chromatographic separation of ...

Microfluidic Glycan Analysis

Microfluidic devices and methods for analyzing glycan profiles of glycoproteins are provided. Some embodiments of the devices comprise a deglycosylation column for cleaving glycans, an optional cleaning column for removing proteins, a trapping column for enriching glycans, and a separation column for resolving glycans. The devices and methods significantly improve the speed and sensitivity of glycan analysis. 2. The device of claim 1 , further comprising a cleaning column capable of binding proteins claim 1 , wherein the cleaning column is configured to be connectable to the deglycosylation column and/or the trapping column by the valve system.3. The device of claim 1 , wherein the enzyme is N-glycosidase F.4. The device of claim 1 , wherein the solid support in the deglycosylation column comprises beads or a monolithic medium.5. The device of claim 1 , wherein the separation column is a liquid chromatography column.6. The device of claim 1 , wherein the separation column is a capillary electrophoresis apparatus.7. The device of that comprises two layers claim 1 , wherein the deglycosylation column is in one layer claim 1 , and the trapping column and separation column are in the other layer.8. The device of that comprises three layers claim 2 , wherein the deglycosylation column is in a first layer claim 2 , the cleaning column is in a second layer claim 2 , and the trapping column and separation column are in a third layer.9. A system for analyzing a sample claim 1 , comprising the device of claim 1 , the switching element claim 1 , and a mass spectrometer.10. The system of claim 9 , wherein the mass spectrometer comprises an electrospray ion source.11. A method for analyzing the carbohydrate moieties of glycoproteins claim 9 , comprising:{'claim-ref': {'@idref': 'CLM-00001', 'claim 1'}, 'applying a sample that may comprise glycoproteins to the device of ;'}digesting the glycoproteins in the deglycosylation column to result in cleaved carbohydrates;binding the ...

MICROFLUIDIC DEVICE

A microfluidic device for separating a phase in a specimen has been described. This is based on a microfluidic trapping area, channels connected to it and integrated inputs and outputs connected onto the channels. An additional integrated input is provided which allows the flow in the device to be controlled and which may prevent leaking of the specimen and the phase. 119.-. (canceled)20. A microfluidic device for separating a phase in a specimen , the microfluidic device comprising a trapping area for capturing the phase of interest , whereby the trapping area is connected on two sides to a first duct and a second duct respectively , both integrated in the microfluidic device , and whereby the microfluidic device furthercomprises a first integrated input connected to the first duct to take the specimen into the trapping area to separate the phase of interest,comprises a first integrated output connected to the second duct to discharge the rest of the specimen, once it has flowed through the trapping area,has a second integrated output connected to a selected duct selected from the first duct or the second duct, to elute the separated phase out of the device via this output,has a second integrated input connected to a first duct or a second duct that is not the selected duct, to connect to a pump to be able to pump the separated phase out of the device, andhas a third integrated input, also connected to the selected duct via a connection located between the connection of the second integrated output on the selected duct and the microfluidic trapping area and via which the liquid flow during separating of the phase and eluting of the phase may be controlled,wherein the third integrated input, when the microfluidic device is in operation and during eluting of the phase of interest, is connected in a circuit to the first integrated input when the third integrated input is in the first duct or to the first integrated output when the third integrated input is in the ...

GAS ANALYTE SPECTRUM SHARPENING AND SEPARATION WITH MULTI-DIMENSIONAL MICRO-GC FOR GAS CHROMATOGRAPHY ANALYSIS

The disclosure describes embodiments of an apparatus including a first gas chromatograph including a fluid inlet, a fluid outlet, and a first temperature control. A controller is coupled to the first temperature control and includes logic to apply a first temperature profile to the first temperature control to heat, cool, or both heat and cool the first gas chromatograph. Other embodiments are disclosed and claimed. 1. An apparatus comprising:a first gas chromatograph including a fluid inlet, a fluid outlet, and a first temperature control; anda controller coupled to the first temperature control, wherein the controller includes logic to apply a first temperature profile to the first temperature control to heat, cool, or both heat and cool the first gas chromatograph.2. The apparatus of wherein the first temperature profile comprises:a first time period during which the temperature control cools the gas chromatograph from an initial temperature to a first temperature; anda second time period during which the temperature control heats the gas chromatograph from the first temperature to a second temperature.3. The apparatus of wherein the first temperature profile further comprises a third time period during which the temperature control holds the gas chromatograph at the first temperature.4. The apparatus of wherein the initial temperature is substantially room temperature claim 2 , the first temperature is lower than room temperature claim 2 , and the second temperature is greater than room temperature.5. The apparatus of wherein the temperature change in the first time period and the temperature change in the second time period are both linear.6. The apparatus of wherein at least one of the temperature change in the first time period and the temperature change in the second time period is non-linear.7. The apparatus of claim 1 , further comprising:a second gas chromatograph having a fluid inlet, a fluid outlet, and a second temperature control, the fluid inlet of ...

DEVICES AND METHODS OF USE THEREOF

Disclosed herein are devices comprising: a sample conduit providing a path for fluid flow extending from a sample inlet to a sample outlet; a thermal housing enclosing the sample conduit, wherein the thermal housing comprises a plurality of measurement regions; and a motorized stage translatable along the thermal housing from a first location to a second location so as to align a detector with one or more of the plurality of measurement regions. Also disclosed are methods of use of the devices described herein. 1. A device comprising:a sample conduit providing a path for fluid flow extending from a sample inlet to a sample outlet;a thermal housing enclosing the sample conduit, wherein the thermal housing comprises a plurality of measurement regions;a detector configured to capture a spectroscopic signal from the sample conduit at one or more of the plurality of measurement regions; anda motorized stage translatable along the thermal housing from a first location to a second location, wherein the detector is coupled to the motorized stage such that the motorized stage is configured to translate the detector along the thermal housing align the detector with one or more of the plurality of measurement regions.25.-. (canceled)6. The device of claim 1 , wherein the sample conduit comprises one or more modules claim 1 , wherein each of the one or more modules comprises a fluid flow path of a predetermined length such that the sample conduit is configured to have a path for fluid flow of a desired length by fluidly connecting one or more modules.7. (canceled)8. The device of claim 1 , wherein the thermal housing is formed from a metal.9. (canceled)10. (canceled)11. The device of claim 1 , wherein the plurality of measurement regions comprise a plurality of voids claim 1 , a plurality of windows comprising a substantially spectroscopically transparent material claim 1 , or a combination thereof claim 1 , such that the plurality of measurement regions are substantially ...

Flow channel structure and measuring device for measurement target liquid

A flow channel structure includes a substrate including a supply flow channel that guides a measurement target liquid toward inside; a separation element accommodating unit that accommodates a separation element that separates components included in the measurement target liquid; and a detection unit that guides the measurement target liquid passing through the separation element accommodating unit, wherein measuring light for measuring information about the components is to be irradiated onto the measurement target liquid. The detection unit includes a measurement flow channel part that guides the measurement target liquid, an incident part that is provided at an end of the measurement flow channel part and that guides the measuring light toward inside the measurement flow channel part, and an emission part that is provided at the other end of the measurement flow channel part and that derives the measuring light from the measurement flow channel part.

Micro Circulatory Gas Chromatography System and Method

A gas chromatography system can include a circulatory loop, a gas inlet positioned along the circulatory loop, a gas outlet positioned along the circulatory loop, a micro column positioned in line with the circulatory loop, and an in-line population sensor positioned in line with the circulatory loop. The in-line population sensor can be configured to detect changes in gas population. The gas inlet and gas outlet can be associated with a gas inlet valve and gas outlet valve, and configured to admit or withdraw gas from the circulatory loop, respectively. A gas sample can be circulated through the circulatory loop for at least one cycle, and a component of the gas sample can be detected using the in-line population sensor. 1. A gas chromatography system comprising:a recirculating loop;a gas inlet positioned along the recirculating loop and configured to admit gas into the recirculating loop;a gas inlet valve associated with the gas inlet, wherein the gas inlet valve can be switched to allow gas to flow into the recirculating loop;a gas outlet positioned along the recirculating loop and configured to withdraw gas from the recirculating loop;a gas outlet valve associated with the gas outlet, wherein the gas outlet valve can be switched to allow gas to flow out of the recirculating loop;a micro column positioned in line with the recirculating loop such that gas cycles around the recirculating loop and through the micro column multiple times; andan in-line population sensor positioned in line with the recirculating loop, the in-line population sensor configured to detect changes in gas population.2. The gas chromatography system of claim 1 , further comprising an in-line micro pump configured to recirculate gas in the recirculating loop.3. The gas chromatography system of claim 1 , further comprising an in-line blocking valve and a controller claim 1 , wherein the controller is configured to open and close the gas inlet valves claim 1 , the gas outlet valves claim 1 , ...

MICROFLUIDIC CHIPS WITH ONE OR MORE VIAS

Microfluidic chips that can comprise thin substrates and/or a high density of vias are described herein. An apparatus comprises: a silicon device layer comprising a plurality of vias, the plurality of vias comprising greater than or equal to about 100 vias per square centimeter of a surface of the silicon device layer and less than or equal to about 100,000 vias per square centimeter of the surface of the silicon device layer, and the plurality of vias extending through the silicon device layer; and a sealing layer bonded to the silicon device layer, wherein the sealing layer has greater rigidity than the silicon device layer. In some embodiments, the silicon device layer has a thickness between about 7 micrometers and about 500 micrometers while a via of the plurality of vias has a diameter between about 5 micrometers and about 5 millimeters. 1. An apparatus , comprising:a silicon device layer comprising a plurality of vias, the plurality of vias comprising greater than or equal to about 100 vias per square centimeter of a surface of the silicon device layer and less than or equal to about 100,000 vias per square centimeter of the surface of the silicon device layer, and the plurality of vias extending through the silicon device layer; anda sealing layer bonded to the silicon device layer, wherein the sealing layer has greater rigidity than the silicon device layer.2. The apparatus of claim 1 , wherein the sealing layer is selected from a group consisting of silicon and glass.3. The apparatus of claim 2 , wherein the silicon device layer has a thickness greater than or equal to about 7 micrometers and less than or equal to about 500 micrometers.4. The apparatus of claim 2 , wherein a via of the plurality of vias has a diameter greater than or equal to about 5 micrometers and less than or equal to about 5 millimeters.5. The apparatus of claim 1 , further comprising a microfluidic element located on a second surface of the silicon device layer claim 1 , wherein the ...

Method and Apparatus for Chromatograph Nano-Flow Fractionator

A nano-flow fractionator apparatus, comprises: one or more sources of mobile phase solvent; a source of auxiliary solvent; a sample injection valve; a chromatographic column having an inner diameter of less than or equal to 75 micro-meters, a column inlet end and a column outlet end; a solvent fraction delivery line comprising: an inlet end that is configured to receive eluate that is emitted from the column outlet end and an outlet end that is configured to dispense the eluate to each of a plurality of sample fraction containers; a fluid junction configured to receive the eluate that is emitted from the column outlet end and to receive a flow of the auxiliary solvent that is delivered from the source of auxiliary solvent and to deliver the eluate and the flow of auxiliary solvent to the solvent fraction delivery line.

Micro Circulatory Gas Chromatography System and Method

A gas chromatography system can include a circulatory loop, a gas inlet positioned along the circulatory loop, a gas outlet positioned along the circulatory loop, a micro column positioned in line with the circulatory loop, and an in-line population sensor positioned in line with the circulatory loop. The in-line population sensor can be configured to detect changes in gas population. The gas inlet and gas outlet can be associated with a gas inlet valve and gas outlet valve, and configured to admit or withdraw gas from the circulatory loop, respectively. A gas sample can be circulated through the circulatory loop for at least one cycle, and a component of the gas sample can be detected using the in-line population sensor. 1. A gas chromatography system comprising:a circulatory loop;a gas inlet positioned along the circulatory loop and configured to admit gas into the circulatory loop, the gas inlet associated with a gas inlet valve;a gas outlet positioned along the circulatory loop and configured to withdraw gas from the circulatory loop, the gas outlet associated with a gas outlet valve;a micro column positioned in line with the circulatory loop; andan in-line population sensor positioned in line with the circulatory loop, the in-line population sensor configured to detect changes in gas population.2. The gas chromatography system of claim 1 , further comprising an in-line micro pump configured to circulate gas in the circulatory loop.3. The gas chromatography system of claim 1 , further comprising an in-line blocking valve and a controller claim 1 , wherein the controller is configured to open and close the gas inlet valves claim 1 , the gas outlet valves claim 1 , and the in-line blocking valves in a sequence to circulate gas in the circulatory loop.4. The gas chromatography system of claim 3 , wherein the system comprises two gas inlets claim 3 , two gas outlets claim 3 , two in-line blocking valves claim 3 , and two micro columns positioned along the ...

MICROSCALE COLLECTOR-INJECTOR TECHNOLOGIES FOR PASSIVE ENVIRONMENTAL VAPOR SAMPLING AND FOCUSED INJECTION

A microscale collector and injector device comprises a microscale passive pre-concentrator (μPP) and a microscale progressively-heated injector (μPHI). The μPP devices comprises first and second substrate portions, a first collection material, a μPP heater, and an outlet. The first substrate portion defines an array of microscale diffusion channels. The first and second substrate portions cooperate to define a first compartment in fluid communication with the diffusion channels. The first collection material is disposed within the first compartment, at least partially surrounding the outlet. The μPP heater is disposed in thermal communication with the second substrate portion. The μPHI device comprises third and fourth substrate portions, a second collection material, and a plurality of μPHI heaters. The third and fourth substrate portions cooperate to define a second compartment. The second collection material is disposed within the second compartment. The μPHI heaters are disposed in thermal communication with the second compartment. 1. A microscale passive pre-concentrator (μPP) device comprising:a first substrate defining an array of microscale diffusion channels;a second substrate coupled to the first substrate, the first substrate and the second substrate cooperating to define a compartment in fluid communication with the array of microscale diffusion channels;a first collection material disposed within the compartment and configured to capture compounds within a first range of vapor pressures;a heating unit disposed in thermal communication with the second substrate and configured to heat the compartment; anda central outlet, the first collection material at least partially surrounding the central outlet, wherein the μPP device is configured to collect a sample at a known rate by using passive diffusion, without use of artificial circulation, and subsequently discharge the sample through the central outlet.2. The μPP device of claim 1 , wherein the first ...

STACKED LAYER-TYPE MEMBER WITH INTEGRATED FUNCTIONAL COMPONENT

A fluidic valve for a sample separation apparatus for separating a fluid, wherein the fluidic valve comprises a stack of connected layer structures, a first conduit within the stack, a second conduit within the stack, a movable body within the stack, and an actuator configured for actuating the movable body to selectively bring the movable body into a flow enabling configuration in which flow of fluid between the first conduit and the second conduit is enabled, or into a flow disabling configuration in which flow of fluid between the first conduit and the second conduit is disabled. 1. A fluidic valve for a sample separation apparatus for separating a fluid , the fluidic valve comprising:a stack of connected layer structures;a first conduit within the stack;a second conduit within the stack;a movable body within the stack;an actuator configured for actuating the movable body to selectively bring the movable body into a flow enabling configuration in which flow of fluid between the first conduit and the second conduit is enabled, or into a flow disabling configuration in which flow of fluid between the first conduit and the second conduit is disabled.2. The fluidic valve according to claim 1 , wherein at least part of the layer structures is configured as a sheet.3. The fluidic valve according to claim 1 , wherein at least part of the layer structures is configured as a patterned layer having one or more recesses constituting at least part of at least one of the first conduit and the second conduit.4. The fluidic valve according to claim 1 , wherein the movable body is configured as a ball.5. The fluidic valve according to claim 1 , wherein the movable body comprises at least one material selected from the group consisting of a ceramic claim 1 , sapphire claim 1 , and ruby.6. The fluidic valve according to claim 1 , wherein the movable body is configured for being forced to sealingly rest on a seat claim 1 , formed by at least a part of the layer structures claim 1 , ...

Laterally perfused chromatography element

A microfluidic chromatography element in which all components are situated in one plane and the mobile phase perfuses the stationary phase laterally.

SAMPLE EXTRACTION APPARATUS WITH MICRO ELUTION BED DESIGN

An apparatus for extracting an analyte from a liquid sample having a container with an entrance, an exit, and a passage therebetween for passage of a liquid sample containing an analyte, the container having a full diameter bed region and a reduced diameter bed region. The container includes a layered construction extending across the passage, having from top to bottom one or more of: (i) an upper flow distributor/support layer, (ii) an upper compression layer, (iii) an extraction layer of microparticulate extraction medium adjacent to the layer (ii), and (iv) a lower compression layer located adjacent to the extraction layer (iii), optionally including one or more air gap layers. At least some of the layers are located in the full diameter bed region, and some of the layers are located in the reduced diameter bed region. The apparatus may have a plurality of containers arranged in an array and/or in series so as to provide multi-stage filtration or extraction. 1. An apparatus for extracting an analyte from a liquid sample comprising:a first microcolumn comprising a first passage, a flow distributor layer extending across the first passage; anda second microcolumn comprising a second passage, an extraction layer extending across the second microcolumn;wherein, the first microcolumn positioned above and in series with the second microcolumn with the first passage in fluid communication with the second passage.2. The apparatus of claim 1 , wherein the first passage of the first microcolumn comprises a first full diameter bed region and a first reduced diameter bed region claim 1 , and the second passage of the second microcolumn comprises a second full diameter bed region and a second reduced diameter bed region.3. The apparatus of claim 2 , wherein the flow distributor layer is located in the first full diameter bed region of the first microcolumn claim 2 , and the extraction layer is located in the second reduced diameter bed region of the second microcolumn.4. The ...

PORTABLE WATER QUALITY INSTRUMENT

A hand-held microfluidic testing device is provided that includes a housing having a cartridge receiving port, a cartridge for input to the cartridge receiving port having a sample input and a channel, where the channel includes a mixture of Raman-scattering nanoparticles and a calibration solution, where the calibration solution includes chemical compounds capable of interacting with a sample under test input to the cartridge and the Raman-scattering nanoparticles, and an optical detection system in the housing, where the optical detection system is capable of providing an illuminated electric field, where the illuminating electric field is capable of being used for Raman spectroscopy with the Raman-scattering nanoparticles and the calibration solution to analyze the sample under test input to the cartridge. 1. A hand-held microfluidic testing device comprising:a. a housing, wherein said housing comprises a cartridge receiving port;b. a cartridge for input to said cartridge receiving port, wherein said cartridge comprises a sample input and a channel, wherein said channel comprises a mixture of Raman-scattering nanoparticles and a calibration solution, wherein said calibration solution comprises chemical compounds capable of interacting with a sample under test input to said cartridge and said Raman-scattering nanoparticles; andc. an optical detection system in said housing, wherein said optical detection system is capable of providing an illuminated electric field, wherein said illuminating electric field is capable of being used for Raman spectroscopy with said Raman-scattering nanoparticles and said calibration solution to analyze said sample under test input to said cartridge.2. The hand-held microfluidic testing device of claim 1 , wherein said chemical compounds are selected from the group consisting of thiols claim 1 , amines claim 1 , silanes claim 1 , polymeric particles claim 1 , metallic particles claim 1 , crown esters claim 1 , cysteamine claim 1 , ...

AUTOMATED SAMPLE FRACTIONATION PRIOR TO MASS SPECTROMETRIC ANALYSIS

Systems, apparatus, methods, and kits are provided for automated mass spectrometric analysis of small volumes of liquid samples, such as biological samples. The systems, apparatus, and kits may be used in facilities where high throughput of samples, as well as reliable and repeatable assay results with little training of staff, are needed. Such facilities include hospital emergency wards. 120.-. (canceled)21. A cartridge for preparing an analyte of interest in a biological sample for mass spectrometry analysis , the cartridge comprising:a housing;a solvent inlet in the housing comprising configured to introduce solvents into the cartridge, at least a portion of the solvent inlet has an asymmetrical shape;a sample introduction port in the housing fluidly connected to the solvent inlet and configured to receive a biological sample comprising an analyte of interest;a chromatography region in the housing fluidly connected to the sample introduction port; andan outlet from the housing fluidly connected to the chromatography region,wherein the solvent inlet of the cartridge is configured to fluidly connect with an outlet of a solvent assembly for mass spectrometry analysis, andwherein the asymmetrical shape of the at least a portion of the solvent inlet is configured to fit together with the outlet of the solvent assembly in a specific orientation to provide an indication that the cartridge is compatible with the solvent assembly for performing an assay to determine the concentration of the analyte of interest.22. The cartridge of claim 21 , further comprising immobilized chromatography media in the chromatography region of the cartridge.23. The cartridge of claim 22 , wherein the immobilized chromatography media corresponds to the assay.24. The cartridge of claim 21 , further comprising one or more filters in the cartridge claim 21 , wherein the one or more filters are configured to remove precipitates and/or insoluble material from the biological sample and yield a ...

METHOD AND APPARATUS FOR DETECTING PARTICLES, LIKE BIOLOGICAL MACROMOLECULES OR NANOPARTICLES

A method of detecting particles (), e. g. proteins, after separation of particles based on their specific features, e.g. charge, size, shape, density, as series of single light scattering events created by the individual particles is described. The particles () are separated from each other along the separation path () and particles have specific arrival times at the target side depending on the particle features. The detecting step comprises an interferometric sensing of the light scattered at individual particles bound or transient in the detection volume (). Parameters of the scattering light signals e.g. the interferometric contrast are analysed for obtaining specific particle features, e.g. size, mass, shape, charge, or affinity of the particles (). Furthermore, a detection apparatus () being configured for detecting particles () is described. 1. A method of detecting particles , comprising the steps ofproviding a sample substance including particles to be detected on a source side of a separation path,subjecting the sample substance to a separation force, so that the particles move along the separation path toward a downstream target side thereof, wherein the particles are separated from each other along the separation path and the particles have specific arrival times at the target side, anddetecting the particles in a detection volume, whereinthe detecting step comprises an interferometric sensing of a series of single light scattering events created by the individual particles in the detection volume, whereinscattering light signals obtained from the interferometric sensing are analyzed for obtaining specific particle features of the particles.2. The method according to claim 1 , whereinthe particles are bound to a detection surface of the detection volume during the detecting step.3. The method according to claim 2 , whereinthe detection surface is arranged with a distance from an opening end of the separation path on the target side thereof.4. The method ...

HIGH SURFACE AREA CHROMATOGRAPHIC DEVICE WITH LOW PRESSURE DROP

A chromatographic device includes a primary channel having a cross-sectional area and characteristic length such that analyte travel within the primary channel is substantially convective. A plurality of secondary channels each having a cross-sectional area and characteristic length such that analyte flow into and out of a secondary channel is substantially diffusive, each of the plurality of secondary channels having an entrance in fluidic communication with the primary channel wherein the entrance intersects the primary channel. 1. A chromatographic method , comprising:providing a chromatographic device comprising a primary channel, and a plurality of secondary channels each having an entrance in fluidic communication with the primary channel wherein the entrance intersects the primary channel; and{'sup': −7', '2', '−12', '2, 'introducing an analyte through each of the primary and secondary channels of the chromatographic device, wherein the Péclet number for the analyte in the primary channel is between 1 and 10 and the Péclet number for the analyte in each of the secondary channels is less than 0.2, wherein a diffusion coefficient of the analyte is between about 1×10m/s to about 1×10m/s.'}2. The chromatographic method of claim 1 , wherein the analyte transport in the primary channel is at least substantially convective claim 1 , and wherein the analyte transport in the secondary channel is at least substantially diffusive.3. The chromatographic method of claim 1 , wherein each of the plurality of secondary channels intersects the primary channel at an angle between about 10 degrees and about 90 degrees.4. The chromatographic method of claim 1 , wherein the cross sectional area of the primary channel varies along the length of the primary channel.5. The chromatographic method of claim 1 , wherein the chromatographic device has a fluid inlet and a fluid outlet and the primary channel follows a substantially linear path from the inlet to the outlet.6. The ...

MICROCOLUMN FOR USE IN GAS CHROMATOGRAPHY

A microcolumn for use in gas chromatography comprises a self-supporting polymer body that functions as a stationary phase and a structural support. The polymer body comprises an enclosed channel having a length L, height h and width w extending therethrough and one or more channel walls surrounding the enclosed channel. The one or more channel walls are integrally formed with the polymer body. The polymer body and the one or more channel walls may comprise a phase-separated polymer composition. 1. A microcolumn for use in gas chromatography , the microcolumn comprising: an enclosed channel having a length L, height h and width w extending therethrough; and', 'one or more channel walls surrounding the enclosed channel, the one or more channel walls being integrally formed with the polymer body., 'a self-supporting polymer body functioning as a stationary phase and a structural support, the polymer body comprising2. The microcolumn of claim 1 , wherein the polymer body and the one or more channel walls comprise a phase-separated polymer composition.3. The microcolumn of claim 1 , wherein the polymer body has a nonuniform thickness about a perimeter of the enclosed channel.4. The microcolumn of claim 1 , wherein the channel walls surrounding the enclosed channel consist of an enclosing wall and one or more supporting walls claim 1 , the enclosing wall having a wall thickness less than that of each of the one or more supporting walls.5. The microcolumn of claim 4 , wherein the enclosing wall comprises a wall thickness of about 100 μm or less claim 4 , and the one or more supporting walls each comprise a wall thickness of at least about 1.5 times the wall thickness of the enclosing wall.6. The microcolumn of claim 1 , wherein a transverse cross-section of the enclosed channel has a polygonal shape.7. The microcolumn of claim 6 , wherein the polygonal shape is a rectangle claim 6 , the polymer body comprising four channel walls surrounding the enclosed channel.8. The ...

MEASUREMENT SYSTEM INCLUDING A NETWORK OF NANOELECTROMECHANICAL SYSTEM RESONATORS

The invention relates to a measurement system including a network of nanoelectromechanical system (NEMS) resonators, characterized in that: each one of said resonators includes: an electrostatic activation device capable of generating a vibration of a beam exposed to said excitation signal, at least one piezoresistive stress gauge made of a doped semiconducting material, extending from the beam so as to detect a movement of said beam, the variation in the electrical resistance of said at least one gauge supplying an output signal; said network includes at least two groups of resonators, each group including at least two resonators having an identical empty resonance frequency, each group of resonators having an empty resonance frequency different from that of each other group; the resonators forming each group are connected in parallel; the groups of resonators forming said network are connected in parallel; said system includes a reading device designed to supply an excitation signal at the network input and to determine the resonance frequency of a group of resonators which is selected by injecting, into said excitation signal, a frequency component corresponding to the empty resonance frequency of each selected group of resonators, and by identifying, in the output signal of the network, a resonance frequency component of the selected group of resonators. 1. A measurement system comprising a network of nanoelectromechanical system (NEMS) resonators , wherein: an input for receiving an excitation signal and an output for supplying an output signal in response to said excitation signal, said output signal exhibiting resonance at the resonant frequency of the resonator,', 'a beam suspended with respect to a support, the natural resonant frequency of the resonator corresponding to the natural resonant frequency of said beam,', 'an electrostatic activation device capable of generating a vibration of said beam under the effect of said excitation signal,', 'at least one ...

MANUFACTURE OF A MICROFLUIDIC COMPONENT BY ADDITIVE MANUFACTURING

A microfluidic component for a sample separation apparatus includes a component body including ceramic and at least one microfluidic structure in the component body. The component body is manufactured by additive manufacturing, in particular by three-dimensional printing. 1. A microfluidic component for a sample separation apparatus , the microfluidic component comprising:a component body comprising ceramic;at least one microfluidic structure in the component body;wherein the component body is produced by a process selected from the group consisting of: additive manufacturing; and three-dimensional printing.2. The microfluidic component according to claim 1 , configured as one selected from the group consisting of: a fluid valve; or a component of a fluid valve; a rotor component of a fluid valve; a stator component of a fluid valve; a sample separation device; a part of a sample separation device; a heat exchanger; and a mixer.3. (canceled)4. The microfluidic component according to claim 1 , wherein the microfluidic component is a one-piece component consisting of the component body.5. The microfluidic component according to claim 1 , wherein the entire component body consists of the ceramic.6. The microfluidic component according to claim 1 , comprising at least one of the following features:wherein the ceramic comprises aluminum oxide and/or zirconium oxide;wherein an aspect ratio of the at least one microfluidic structure is in a range selected from the group consisting of: at least 50; at least 100; and between 200 and 400;wherein an inner diameter of the at least one microfluidic structure is in a range selected from the group consisting of: between 0.05 mm and 1 mm; and between 0.1 mm and 0.5 mm;wherein the at least one microfluidic structure comprises at least one of a group consisting of: a completely circumferentially confined microfluidic channel; and a microfluidic groove.7. A green body for producing the microfluidic component of claim 1 , the green ...

POLYMER MICROCOLUMN FOR GAS OR VAPOR SEPARATION, CHROMATOGRAPHY, AND ANALYSIS

In an aspect, a method for forming a microcolumn comprises steps of: (a) providing a sacrificial fiber; (b) forming a microcolumn body around said sacrificial fiber; and (c) removing said sacrificial fiber from said microcolumn body such that a hollow channel is formed within said microcolumn body via removal of said sacrificial fiber. In any embodiment of the methods disclosed herein for forming a microcolumn, said hollow channel extends through said microcolumn body and is continuous between a first end and a second end. The first end may be an inlet and the second end may be an outlet, for example, allowing for a mobile phase to enter the hollow channel via the first end and exit via the second end. 1. A method for forming a microcolumn , the method comprising steps of:a. providing a sacrificial fiber;b. forming a microcolumn body around said sacrificial fiber; andc. removing said sacrificial fiber from said microcolumn body such that a hollow channel is formed within said microcolumn body via removal of said sacrificial fiber.2. The method of claim 1 , wherein said hollow channel extends through said microcolumn body claim 1 , and said hollow channel is continuous between a first end and a second end of said hollow channel.3. The method of claim 1 , wherein said hollow channel has a three-dimensional geometry.4. The method of claim 2 , wherein a cross-sectional shape of said hollow channel is circular between said first end and said second end of said hollow channel.5. The method of claim 4 , wherein said hollow channel has an inner diameter and an outer diameter defining a thickness of a wall of said hollow channel claim 4 , and wherein said thickness of said wall is substantially uniform between said first end and said second end of said hollow channel; and said wall comprises a stationary phase material.6. (canceled)7. The method of claim 2 , wherein an inner surface of said hollow channel is substantially smooth.8. The method of claim 1 , wherein said ...

MICROFLUIDIC HPLC-CHIP FOR GLYCOPEPTIDE ANALYSIS WITH INTEGRATED HILIC ENRICHMENT

A microfluidic device for glycopeptide analysis includes an enrichment column capable of binding carbohydrates; a trapping column capable of binding peptides, wherein the trapping column is configured to be connected downstream of the enrichment column; a separation column, wherein the separation column is configured to be connected downstream of the trapping column; and a plurality of ports configured to work with a switching device to form a plurality of flow paths, wherein one of the plurality of flow paths allows the trapping column to be in fluid communication with the separation column. A method for glycopeptide analysis using a microfluidic device comprising a trapping column and a separation column, the method includes applying a sample of peptides to the microfluidic device; trapping the peptides on the trapping column; eluting the peptides from the trapping column into the separation column; and separating the peptides on the separation column. 1. A microfluidic device for glycopeptide analysis , comprising:an enrichment column comprising a stationary phase capable of binding carbohydrates;a trapping column comprising a stationary phase capable of binding peptides, wherein the trapping column is configured to be connected downstream of the enrichment column;a separation column comprising a stationary phase capable of separating peptides, wherein the separation column is configured to be connected downstream of the trapping column; anda plurality of ports configured to work with a switching device to form a plurality of flow paths, wherein one of the plurality of flow paths allows the trapping column to be in fluid communication with the separation column.2. The microfluidic device of claim 1 , wherein the enrichment column comprises a hydrophilic interaction (HILIC) stationary phase.3. The microfluidic device of claim 1 , wherein the trapping column comprises a hydrophilic interaction (HILIC) stationary phase claim 1 , a reversed phase stationary phase ...

DEVICE COMPRISING A FLUID CHANNEL PROVIDED WITH AT LEAST ONE MICRO OR NANOELECTRONIC SYSTEM AND METHOD FOR CARRYING OUT SUCH A DEVICE

Device including a substrate including at least one microelectronic and/or nanoelectronic structure (NEMS) having a sensitive portion and a fluid channel. The fluid channel includes two lateral walls, an upper wall connecting the two lateral walls, a lower wall formed by the substrate, and at least two openings in order to provide a circulation in the fluid channel, with the openings being defined between the two lateral walls, with the structure being located inside the fluid channel. Electrical connection lines extend between the structure and the outside of the fluid channel, with the connection lines being carried out on the substrate and passing under the lateral walls. The device also includes an intermediate layer having a planar face in contact with base faces of said lateral walls. The connection lines are at least partially covered by the intermediate layer at least immediately above base faces of the lateral walls. The lateral walls are made sealingly integral on the substrate by a sealing layer on the intermediate layer. 1. A device comprising:a substrate comprising at least one microelectronic and/or nanoelectronic structure comprising at least one suspended portion,a cover,a fluid channel defined between said substrate and the cover, said fluid channel comprising two lateral walls and an upper wall connecting the two lateral walls formed by said cover and a lower wall formed by said substrate and at least two openings in order to provide a circulation in said channel, said microelectronic and/or nanoelectronic structure being located inside the fluid channel,an intermediate layer comprising a face in contact with base faces of said lateral walls, said face of the intermediate layer having an aptitude for sealing with the base faces,at least one electrical connection line extending between said microelectronic and/or nanoelectronic structure and the outside of the fluid channel, said connection line being carried out on the substrate and passing under ...

Chromatography Column Assembly

Described is a chromatographic column assembly that includes an outer tube comprising a metal, a first conduit disposed within the outer tube, a second conduit disposed within the outer tube, and a first joint located between the first conduit and the second conduit. The outer tube is deformed by a first uniform radial crimp at a longitudinal location along the outer tube that surrounds the first conduit on a first side of the first joint, and a second uniform radial cramp at a longitudinal location along the outer tube that surrounds the second conduit on a second side of the first joint. The first and second uniform radial cramps form a fluid-tight seal between the first conduit and the second conduit and each have a substantially flat base region over which a diameter of the outer tube is reduced for a non-zero longitudinal length. 1. A chromatographic column assembly , comprising:an outer tube comprising a metal;an intermediate tube comprising a polymeric material and disposed within the outer tube;a sorbent bed tube disposed within the intermediate tube and having a first end and a second end opposite the first end;a sorbent bed disposed within the sorbent bed tube; anda first inner tube disposed within the intermediate tube and having a first inner tube end abutting the first end of the sorbent bed tube, thereby defining a first joint;wherein the outer tube is deformed by a first uniform radial crimp at a longitudinal location along the outer tube that surrounds the first inner tube on a first side of the first joint, wherein the outer tube is deformed by a second uniform radial cramp at a longitudinal location along the outer tube that surrounds the sorbent bed tube on a second side of the first joint, andwherein the first and second uniform radial cramps form a fluid-tight seal between the first inner tube end and the first end of the sorbent bed tube.2. The chromatographic column assembly of claim 1 , wherein the first and second uniform radial crimps each ...

APPARATUS FOR ASSAY, SYNTHESIS AND STORAGE, AND METHODS OF MANUFACTURE, USE, AND MANIPULATION THEREOF

The invention features methods of making devices, or “platens”, having a high-density array of through-holes, as well as methods of cleaning and refurbishing the surfaces of the platens. The invention further features methods of making high-density arrays of chemical, biochemical, and biological compounds, having many advantages over conventional, lower-density arrays. The invention includes methods by which many physical, chemical or biological transformations can be implemented in serial or in parallel within each addressable through-hole of the devices. Additionally, the invention includes methods of analyzing the contents of the array, including assaying of physical properties of the samples. 111-. (canceled)12. A method of creating a chemical array , the method comprising:a) providing a platen having a plurality of through-holes and two opposing surfaces;b) applying a mask to one or both surfaces of the platen to block at least some of the through-holes, while leaving other through-holes open;c) exposing a surface of the platen to a reagent so that the reagent enters at least one of the open through-holes; andd) repeating steps b) and c) with at least one different mask and at least one different reagent to create a chemical array.13. The method of claim 12 , wherein the mask is made of a polymer claim 12 , an elastomer claim 12 , paper claim 12 , glass claim 12 , or a semiconductor material.14. The method of claim 12 , wherein the mask comprises mechanical valves claim 12 , pin arrays claim 12 , or gas jets.15. The method of claim 12 , wherein the applying step forms a hermetic seal between the mask and the platen.16. The method of claim 12 , wherein the reagent is a liquid claim 12 , a gas claim 12 , a solid claim 12 , a powder claim 12 , a gel claim 12 , a solution claim 12 , a suspension claim 12 , a cell culture claim 12 , a virus preparation claim 12 , or electromagnetic radiation.17. The method of claim 12 , wherein the mask is translated to expose ...

PRODUCTION OF CHEMICAL REACTORS

A method for producing a chemical reactor, wherein the chemical reactor comprises one or more effective channels which comprise pillar structures, an input connected to one of the effective channels to allow fluid/gas into the effective channels and an output connected to one of the effective channels to remove at least one component of the liquid/gas. The method comprises obtaining an initial design of the reactor, further introducing into the initial design at least a structured area positioned adjacent to an effective channel of the one or more effective channels located at the edge of the initial design, the structured area not being fluidly connected to one of the effective channels, to obtain a further design and the production of the reactor according to the further design 123.-. (canceled)24. A method for producing a chemical reactor implemented on a substrate , the chemical reactor comprising:one or more effective channels for transporting a liquid and/or gas during use of the chemical reactor, the channels optionally comprising pillar structures;an input connected to one of the one or more effective channels to allow liquid/gas into the effective channels;an output connected to one of the one or more effective channels to remove at least one component of the liquid and/or gas from the effective channels;the method comprisingobtaining an initial design of the chemical reactor with the one or more effective channels, the input and the output;further introducing into the initial design at least a portion of a structured area positioned adjacent to an effective channel of the one or more effective channels located at the edge of the initial design, the structured area not being fluidly connected to one of the effective channels, to obtain a further design;the production of the chemical reactor according to the further design, the production comprising the generation of an electrical current density in the substrate, and wherein the structured area at least ...

COLUMN FOR MICRO GAS CHROMATOGRAPHY

Disclosed is a separation column for micro gas chromatography, having superior separation performance and including a microchannel formed on a substrate and having a serpentine shape, and bumps formed on the surface of the microchannel, wherein the bumps are alternately disposed to face each other on the surface of the microchannel. In the separation column for micro gas chromatography, a sufficient pressure drop occurs, and thus an M interaction between a gas mixture to be analyzed and a stationary phase in the column sufficiently takes place, whereby individual gas components are efficiently separated from the gas mixture, discharged from the column, and detected. 1. A separation column for micro gas chromatography , comprising:a microchannel formed on a substrate and having any one shape selected from among a rectangular shape, a circular shape, and a serpentine shape; andbumps formed on a surface of the microchannel,wherein the bumps are alternately disposed to face each other on the surface of the microchannel.2. The separation column of claim 1 , wherein the microchannel has a channel width of 140 to 200 μm and a channel depth of 300 to 450 μm.3. The separation column of claim 1 , wherein a ratio of a distance claim 1 , r2 claim 1 , between a top of the bumps and a wall of the microchannel to a height claim 1 , r1 claim 1 , of the bumps formed on the wall of the microchannel claim 1 , which is r2/r1 claim 1 , ranges from 1.0 to 1.5.4. The separation column of claim 1 , wherein a ratio of a distance claim 1 , d claim 1 , between the bumps to a height claim 1 , r1 claim 1 , of the bumps formed on a wall of the microchannel claim 1 , which is d/r1 claim 1 , ranges from 3 to 5. This application claims the priority of the Korean Patent Applications NO 10-2017-0057569 filed on May 8, 2017, in the Korean Intellectual Property Office, the disclosure of which is incorporated herein by reference.The present invention relates to a column for use in micro gas ...

BIOCHEMICAL ANALYSIS APPARATUS AND ROTARY VALVE

An analysis apparatus for performing biochemical analysis of a sample using nanopores comprises: a sensor device that that supports plural nanopores, reservoirs holding material for performing the analysis; a fluidics system; and plural containers for receiving respective samples, all arranged in a cartridge that is removably attachable to an electronics unit arranged to generate drive signals to perform signal processing circuit to generate output data representing the results of the analysis. The fluidics system supplies samples selectively from the containers to the sensor device using a rotary valve. In one valve, a stator defines a plurality of first ports arranged around the rotational axis and a collection chamber extending in around the axis of rotation in communication with a second port. A rotor provides a passage extending between the collection chamber and a position in communication with any one of the plurality of first ports. In another valve a stator defining a plurality of first ports in an annular surface facing the rotational axis, and a rotor is mounted inside a liner arranged between the annular surface of the stator and a facing annular surface of the rotor. The liner has a greater compliance than the rotor and stator to facilitate sealing. 1. An analysis apparatus for performing biochemical analysis of a sample using nanopores , the analysis apparatus comprising:a sensor device that is capable of supporting plural nanopores and being operable to perform biochemical analysis of a sample using the nanopores;at least one reservoir for holding material for performing the biochemical analysis; a fluidics system configured to controllably supply material from the at least one reservoir to the sensor device; anda plurality of containers for receiving respective samples, the fluidics system being configured to supply the samples selectively from the containers to the sensor device.2. An analysis apparatus according to claim 1 , wherein the analysis ...

LIQUID-CHROMATOGRAPHY APPARATUS HAVING DIFFUSION-BONDED TITANIUM COMPONENTS

An HPLC apparatus includes a heat exchanger formed from diffusion-bonded first and second titanium substrates. At least two conduits for counterflow are defined between the first and second substrates. 116-. (canceled)17. An HPLC apparatus , comprising:a separation column; and a cell body comprising titanium or a titanium alloy, the cell body defining a flow channel, an inlet port to receive an eluent from the separation column, and an outlet port,', 'a window disposed adjacent to an inlet of the flow channel,', 'a titanium-based gasket disposed between the window and the cell body and diffusion bonded to the cell body to define a fluid circuit that connects the inlet of the flow channel to the inlet port., 'a flow cell downstream of the separation column, the flow cell comprising'}18. The HPLC apparatus of claim 17 , wherein the window is diffusion bonded to the gasket.19. The HPLC apparatus of claim 17 , wherein the window in anodically bonded to the gasket.20. (canceled)21. The HPLC apparatus of claim 17 , wherein the flow cell further comprises a second window disposed adjacent to an outlet of the flow channel cell body claim 17 , and a second gasket disposed between the second window and the outlet of the flow channel and diffusion bonded to the cell body.22. (canceled)23. The HPLC apparatus of claim 18 , wherein the window and the gasket further comprise a gold coating layer.24. The HPLC apparatus of claim 17 , wherein the titanium-based gasket comprises Ti-6Al-4V.25. The HPLC apparatus of claim 17 , further comprising a protective coating formed as a surface layer on the flow channel.26. The HPLC apparatus of claim 25 , wherein surface layer is formed of TiN claim 25 , TiCN or TiAlN.27. An LC apparatus claim 25 , comprising:a detector cell,a separation column, anda heat exchanger, the detector cell or separation column disposed downstream of the heat exchanger, to receive a temperature regulated liquid from the heat exchanger, wherein the detector cell or the ...

PROTECTION DEVICE OF INTEGRATED COLUMN USED IN CAPILLARY LIQUID CHROMATOGRAPHY-ELECTROSPRAY IONIZATION-MASS SPECTROMETRY

A device for protecting electrospray ionization (ESI) emitter tip made on a fused silica capillary nanoscale liquid chromatography (nanoLC) column for robust use of the nanoLC-ESI integrated column. 1. A nanoLC-ESI integrated column protection device comprises a protection sleeve tube to protect the integrated nanoLC column ESI emitter , a protection tube to protect the integrated nanoLC column , and a sliding tube to provide sliding friction for pulling/pushing said protection sleeve tube.2. The nanoLC-ESI integrated column protection device of claim 1 , wherein said protection sleeve tube has a length ranging from 1 mm to full length of the integrated column.3. The nanoLC-ESI integrated column protection device of claim 2 , wherein said protection sleeve tube is made of plastic claim 2 , metal claim 2 , rubber claim 2 , or other materials.4. The nanoLC-ESI integrated column protection device of claim 3 , wherein said protection sleeve tube is in the shape of round claim 3 , square claim 3 , or others.5. The nanoLC-EST integrated column protection device of claim 4 , wherein said protection sleeve tube in the shape of round has an inner diameter ranging from 0.01 mm to 3 mm and an outside diameter ranging from 0.01 mm to 30 mm.6. The nanoLC-ESI integrated column protection device of claim 4 , wherein said protection sleeve tube in the shape of square and others has an inside dimension ranging from 0.01 mm to 3 mm and an outside dimension ranging from 0.01 mm to 30 mm.7. The nanoLC-ESI integrated column protection device of claim 1 , wherein said protection tube has a length ranging from zero to full length of the nanoLC column. (Said protection tube length of zero means that said protection sleeve tube slides directly along the fused silica capillary nanoLC column.)8. The nanoLC-ESI integrated column protection device of claim 7 , wherein said protection tube to protect the nanoLC column is made of plastic materials.9. The nanoLC-ESI integrated column protection ...

MICROFLUIDIC BIOSENSOR FOR ALLERGEN DETECTION

The present application relates to biosensors and methods for detecting and/or quantifying a target analyte such as a target allergen or toxin. In some embodiments, the biosensors use an allergen- or toxin-binding molecule conjugated to a fluorescent label such as a quantum dot that adheres to and is quenched by graphene oxide in the absence of the allergen or toxin. 1. A probe composition comprising:a probe comprising an allergen- or toxin-binding molecule conjugated to a fluorophore, andgraphene oxide,wherein the probe adheres to graphene oxide such that the fluorophore is quenched through fluorescence energy resonance transfer (FRET) and the probe dissociates from graphene oxide when bound to a target allergen or toxin.2. The probe composition of claim 1 , wherein the allergen- or toxin-binding molecule is an aptamer or antibody and the fluorophore is a quantum dot.4. The probe composition of claim 1 , wherein the allergen-binding molecule selectively binds to an allergen selected from peanut allergens claim 1 , egg allergens claim 1 , legume allergens claim 1 , milk allergens claim 1 , seafood allergens claim 1 , mustard allergens claim 1 , sesame allergens claim 1 , soy allergens claim 1 , tree nut allergens and wheat allergens.5. The probe composition of claim 4 , wherein the peanut allergen is Ara h 1 claim 4 , the egg allergen is lysozyme claim 4 , and/or the legume allergen is lupine.6. The probe composition of claim 5 , wherein the allergen-binding molecule is an aptamer comprising a nucleic acid molecule with a nucleic acid sequence having at least 90% sequence identity to SEQ ID NO: 1 claim 5 , SEQ ID NO: 2 or SEQ ID NO: 3.7. The probe composition of claim 1 , wherein the toxin-binding molecule selectively binds to a seafood toxin selected from okadaic acid and brevetoxin.8. The probe composition of claim 7 , wherein the toxin-binding molecule is an aptamer comprising a nucleic acid molecule with a nucleic acid sequence having at least 90% sequence ...

MICRO FLUIDIC STRUCTURES

A micro fluidic system includes a substrate, and, provided on said substrate, at least one flow path interconnecting with functional means in which liquid samples can be treated by desired procedures. The flow paths are laid out to form a pattern for the transport of liquid samples to and from said functional means. These flow paths comprise a plurality of micro posts protruding upwards from said substrate, the spacing between the micro posts being small enough to induce a capillary action in a liquid sample applied anywhere within any of said flow paths, so as to force said liquid to move from where said liquid sample was applied. 1. A method for measuring the amount of analyte in a biological sample , the method comprising the steps of: a substrate having a non-porous surface;', 'a plurality of microposts protruding from the non-porous surface of the substrate, said microposts defining at least one open flow path in which liquid samples can be treated by desired procedures, wherein the at least one flow path is laid out for transport of liquid samples, the at least one flow path extending in a lateral direction which is transverse to the protruding microposts, wherein cross sections of the microposts and the center to center spacing between each of the microposts spontaneously induces a passive capillary action in a liquid sample applied to the at least one flow path, so as to force the liquid to move laterally away from where the liquid sample was applied, the microposts further including antibodies having an affinity for the analyte in the sample;', 'a sample receiving area disposed prior to the at least one flow path and fluidly connected therewith; and', 'a sample collecting sink disposed after the at least one flow path on the substrate and fluidly connected with the at least one flow path, the sample collecting sink having an area which is larger than the area of the at least one flow path extending from the sample receiving area, the sink having a plurality ...

Microfluidic device

A microfluidic device for analysing a specimen comprises a loading area for loading the specimen of interest and an analytical column. The loading area is connected on two sides to a first duct and a second duct respectively, both integrated in the microfluidic device. The microfluidic device comprises a first integrated input connected to the first duct to take the specimen into the loading area, a first integrated output connected to the second duct to discharge the rest of the specimen, once it has flown through the loading area, and a second integrated output downstream the analytical column. The first integrated output is arranged for during a first loading period of time being in circuit connected to the first integrated input so as to load the sample into the loading zone of the device while preventing loss of specimen during loading of the sample into the analytical column.

SYSTEM FOR DETECTING LIQUID ANALYTES

A sample cartridge for a liquid chromatography device includes a microfluidic chip. A collector in the microfluidic chip includes a collector flow channel and a first window for acquisition of spectral data from a sample in the collector flow channel. 1. A sample cartridge for a liquid chromatography device comprising:a microfluidic chip; anda collector formed in the microfluidic chip comprising a collector flow channel and a first window for acquisition of spectral data from a sample in the collector flow channel.2. The sample cartridge of claim 1 , further comprising:a case enclosing the sample cartridge, the case including a port, the port aligned with the first window.3. The sample cartridge of claim 1 , wherein the microfluidic chip further comprises a separator column in fluid communication with the collector flow channel.4. The sample cartridge of claim 3 , wherein the separator column comprises a separator structure and a separator flow channel through the separator structure.5. The sample cartridge of claim 4 , wherein the separator structure comprises a plurality of three-triangular pillars claim 4 , the three-triangular pillars arranged in a plurality of rows to define the separator flow channel.6. The sample cartridge of claim 5 , wherein:in each row of three-triangular pillars in the separator structure, a spacing from a center of one three-triangular pillar to a center an adjacent three-triangular pillar is a distance w; anda subsequent row of three-triangular pillars is laterally offset from a preceding row of three-triangular pillars by a distance w/2.7. The sample cartridge of wherein at least some of the plurality of three-triangular pillars comprise a seven-sided structure.8. The sample cartridge of claim 1 , wherein the microfluidic chip further comprises a switching valve.9. The sample cartridge of claim 8 , wherein the switching valve is in fluid communication with the separator column in at least one position.10. The sample cartridge of claim ...

MEMS FLOW CONTROL CHIP FOR GAS CHROMATOGRAPHY

A micro-electro-mechanical system (MEMS) flow control chip that can control the flow of gas and be configured to operate in a gas chromatography system are disclosed. The MEMS flow control chip can include at least one inlet port in the chip, at least one outlet port in the chip, at least one flow channel between the inlet and outlet ports, and at least one pneumatic valve in the chip for controlling a flow of gas through the flow channel and between the inlet port and outlet ports. Advantageously, the MEMS flow control chip can be positioned in an oven of the a gas chromatography system and have a temperature approximately the same as one or more chromatographic columns of the system. 1. A MEMS flow control chip comprising: a first inlet port in the chip , a first outlet port in the chip , a first flow channel between the first inlet port and the first outlet port in the chip , a first pneumatic valve in the chip for controlling a flow of gas through the first flow channel and between the first inlet port and the first outlet port , wherein the flow channel has a cross sectional area of between about 1 μmand 2.5 mm.2. The MEMS flow control chip of claim 1 , comprising: (i) a flow channel chip including the first inlet port claim 1 , the first outlet port claim 1 , the first flow channel and a valve seat positioned in the first channel claim 1 , (ii) an actuator chip including a chamber aligned over the valve seat and (iii) a diaphragm positioned between the chamber of the actuator chip and the valve seat of the flow channel chip claim 1 , wherein the diaphragm can form a seal against the valve seat to prevent gas from flowing through the first channel and includes a moveable portion that can deform along an axial direction into the chamber to allow fluid communication through the first flow channel and between the first inlet and first outlet ports.3. The MEMS flow control chip of claim 2 , wherein the flow channel chip is composed of a glass and the diaphragm is ...

Progressive Cellular Architecture For Microfabricated Gas Chromatograph

In order to achieve a system capable of analyzing a wide range of compounds while saving time and energy consumption, a progressive cellular architecture is presented for vapor collection and gas chromatographic separation. Each cell includes a preconcentrator and separation column that are adapted for collecting and separating compounds only within a specific volatility range. A wide volatility range can therefore be covered by the use of multiple cells that are cascaded in the appropriate order. The separation columns within each cell are short enough to reduce the heating and pumping requirements. The gas flow for vapor collection and separation is provided by low-power gas micropumps that use ambient air. The system is also configurable to incorporate capabilities of detecting and reducing vapor overload. The progressive cellular architecture directly address the compromise between low power and broad chemical analyses. 1. A gas chromatography architecture , comprising:two or more preconcentrators coupled together in series, the two or more preconcentrators having an input configured to receive an analyte and an output, each preconcentrator comprising a chamber through which the analyte passes and sorbent material within the chamber;a separation column associated with each preconcentrator of the two or more preconcentrators, each separation column having a channel through which the analyte passes; anda three-port fluidic coupling device disposed at an inlet of each preconcentrator in the two or more preconcentrators, the first port of the fluidic coupling device configured to receive an incoming analyte, the second port of the fluidic coupling device fluidly coupled to an inlet of an associated preconcentrator and a third port of the fluidic coupling device fluidly coupled to an associated separation column.2. The gas chromatography architecture of further comprises a pump fluidly coupled to the output of the two or more preconcentrators.3. The gas ...

Thermal conductivity sensing device, Methods for operation and uses of the Same

A thermal conductivity sensing device () is disclosed, along with a method for operation of the thermal conductivity sensing device and use of the thermal conductivity sensing device in a system for gas chromatography and a method of carrying out gas chromatography. The thermal conductivity sensing device is for use in sensing one or more gaseous components in a flowing gaseous environment. The device has a first sensor (B) and a second sensor (A) for exposure to the same flowing gaseous environment (G). The first sensor has an associated flow altering means () to affect gas flow at least at part of the surface of the first sensor, to be different to gas flow at the surface of the second sensor. Each sensor provides an output relating to heat transfer between a surface of the sensor and the gaseous environment. The device is operable to compare outputs of the first and second sensors. The sensor is able to reduce the effects of bulk convection of the flowing gas on thermal conductivity measurements. 1. A thermal conductivity sensing device for use in sensing one or more gaseous components in a flowing gaseous environment , the device having a first sensor and a second sensor for exposure to the gaseous environment , each sensor providing a surface for thermal contact with the gaseous environment , each sensor providing an output relating to heat transfer between said surface and the gaseous environment , the first sensor having an associated flow altering means to affect gas flow at least at part of said surface of the first sensor , to be different to gas flow at the surface of the second sensor , the device being operable to compare outputs of the first and second sensors.2. The thermal conductivity sensing device according to claim 1 , wherein the first and second sensors are provided with respective heating elements.3. The thermal conductivity sensing device according to claim 2 , wherein at least one of the heating elements is made of tungsten or a tungsten- ...

APPARATUS FOR ASSAY, SYNTHESIS AND STORAGE, AND METHODS OF MANUFACTURE, USE, AND MANIPULATION THEREOF

The invention features methods of making devices, or “platens”, having a high-density array of through-holes, as well as methods of cleaning and refurbishing the surfaces of the platens. The invention further features methods of making high-density arrays of chemical, biochemical, and biological compounds, having many advantages over conventional, lower-density arrays. The invention includes methods by which many physical, chemical or biological transformations can be implemented in serial or in parallel within each addressable through-hole of the devices. Additionally, the invention includes methods of analyzing the contents of the array, including assaying of physical properties of the samples. 150-. (canceled)51. A device for filling through-holes in a platen , the device comprising:a platen comprising a two-dimensional array of through-holes, each of the through-holes configured to receive at least one reagent;a nozzle having an aperture of a suitable size to inject a sample into a plurality of the array of through-holes; anda valve configured to control a flow of a sample through the nozzle;wherein the nozzle is configured to move in at least two dimensions to predetermined positions over respective ones of the plurality of through-holes.52. The device of claim 51 , wherein the nozzle is positioned so as to contact the platen.53. (canceled)54. The device of claim 51 , further comprising a computer that controls the valve and controls the predetermined positions of the nozzle over the plurality of through-holes.5563-. (canceled)64. A method of storing a plurality of samples in an assay-ready claim 51 , high-density format claim 51 , the method comprising:a) providing a platen having a plurality of through-holes;b) loading the through-holes with said samples dissolved in a mixture comprising two solvents, a first solvent having a low vapor pressure and a second solvent having a higher vapor pressure; andc) evaporating the second solvent to result in a plurality ...

Spin coated stationary phase microfabricated gas chromatographic columns

Presented herein is a new concept of uniformly spin coating a flat surface with a stationary phase and creating a gas chromatography column by pressing a grooved lid, with micro-stamped ridges, down onto the coated substrate. The lids are molded out of commercially available rigid materials including epoxies so that when pressed onto a flat surface it will create an air tight seal. The epoxy material is rendered inert by a thin layer of gold.

DEVICE FOR PREPARING A LIQUID SAMPLE FOR A GAS CHROMATOGRAPH

A device for preparing a liquid sample for a direct injection of a corresponding gaseous sample to a micro-gas chromatograph includes: a fluid space and a gas space, which spaces are separated by a semipermeable separating layer, the fluid space including a supply line for the liquid sample, and the gas space having an outlet connectable with the gas chromatograph. The fluid space and/or the gas space is associated with at least one heating element. The device absorbs a sample volume of approximately 10 μl to 30 μl. The separating layer has a thickness of 10 μl to 300 μl and pores having a size between 0.05 μl and 5 μl. 1. Device A device for preparing a liquid sample for a direct injection of a corresponding gaseous sample to a micro-gas chromatograph , the device comprising:having a fluid space and a gas space, which spaces are separated by a semipermeable separating layer, the fluid space comprising a supply line for the liquid sample, and the gas space having an outlet connectable with the gas chromatograph,wherein the fluid space and/or the gas space is associated with at least one heating element,wherein the device is configured to absorb a sample volume of approximately 10 μl to 30 μl, andwherein the separating layer has a thickness of 10 μm to 300 μm and pores having a size between 0.05 μm and 5 μm.2. The device according to claim 1 , wherein the separating layer is selectively permeable from at least one side.3. (canceled)4. The device according to claim 1 , the separating layer is a membrane.5. The device according to claim 4 , wherein the membrane consists of one of the following materials: polytetrafluoroethylene claim 4 , polyvinylidene fluoride claim 4 , polyester claim 4 , polysulphone claim 4 , cellulose derivative claim 4 , polyamide claim 4 , polyacrylate claim 4 , or polypropylene.6. The device according to claim 5 , wherein the membrane is elongated.7. The device according to claim 1 , wherein the separating layer comprises at least two layers.8. ...

SYSTEM FOR DETECTING LIQUID ANALYTES

A sample cartridge for a liquid chromatography device includes a microfluidic chip. A collector in the microfluidic chip includes a collector flow channel and a first window for acquisition of spectral data from a sample in the collector flow channel. 120.-. (canceled)21. A microfluidic chip for a liquid chromatography device comprising: a collector flow channel;', 'a first window for acquisition of spectral data from a sample in the collector flow channel; and', 'a reflector supported on a surface of the collector flow channel., 'a collector formed in the microfluidic chip comprising22. The microfluidic chip of claim 21 , wherein the reflector is a mirror.23. The microfluidic chip of claim 21 , wherein the first window is arranged on a first side of the collector and the reflector is arranged on a second side of the collector claim 21 , the second side opposite of the first side claim 21 , such that a portion of the collector flow channel is between the first window and the reflector.24. The microfluidic chip of claim 23 , wherein the reflector is arranged parallel to the first window.25. The microfluidic chip of claim 24 , wherein a direction of flow through the portion of the collector flow channel between the first window and the reflector is parallel to the first window and the reflector.26. The microfluidic chip of claim 24 , wherein a direction of flow through the portion of the collector flow channel between the first window and the reflector is perpendicular to the first window and the reflector.27. The microfluidic chip of claim 26 , wherein a cross section of the collector channel is z-shaped as viewed from a side of the collector.28. The microfluidic chip of claim 21 , wherein the microfluidic chip further comprises a separator column in fluid communication with the collector flow channel.29. The microfluidic chip of claim 28 , wherein the microfluidic chip further comprises a switching valve in fluid communication with the separator column in at least ...

Micromachined titanium for high pressure microfluidic applications

In accordance with the invention, a method for making microfluidic structures in bulk titanium is disclosed. Specific microfluidic structures include HPLC structures.

Fluidic device with planar coupling member

A fluidic device (300, 304) for providing fluidic connections is described. The fluidic device (300, 304) comprises a fluid conduit and a planar coupling member (301, 305) with a fluid port (303, 307), the fluid port (303, 307) being fluidically connected with the fluid conduit. A contour (302, 306) of the planar coupling member is in a predefined relationship with the fluid port's position.

Microfluidic devices and methods of use

A microfluidic device comprises pumps, valves, and fluid oscillation dampers. In a device employed for sorting, an entity is flowed by the pump along a flow channel through a detection region to a junction. Based upon an identity of the entity determined in the detection region, a waste or collection valve located on opposite branches of the flow channel at the junction are actuated, thereby routing the entity to either a waste pool or a collection pool. A damper structure may be located between the pump and the junction. The damper reduces the amplitude of oscillation pressure in the flow channel due to operation of the pump, thereby lessening oscillation in velocity of the entity during sorting process. The microfluidic device may be formed in a block of elastomer material, with thin membranes of the elastomer material deflectable into the flow channel to provide pump or valve functionality.

Detector cell

A miniaturised detector cell (4) having a measurement chamber volume of 25 fl-1 mu l is described. Despite the miniaturised design, the detector cell has an optical wavelength of approximately 0.1-100 mm. In this case, the incident measuring light (R) is multiply reflected at the interior walls (23, 25, 26) of the interaction region (21) before it once again leaves the detector cell (4). It is preferred to produce the detector cell (4) photolithographically from silicon or quartz. <IMAGE>

Electrospray interface to a microfluidic substrate

An apparatus for chemical separations includes a microfluidic substrate having an outlet aperture for outputting an eluent of a sample, a spray unit having an inlet to receive the eluent and an outlet to emit a spray of the eluent, and a force-applying unit. The spray unit has a deformable portion defining the inlet and having an elastic modulus that is lower than an elastic modulus of the microfluidic substrate. The force-applying unit, such as a spring, is disposed to urge the deformable portion in contact with the substrate to form a substantially fluid-tight seal.

Magnetic bead-based arrays

The present invention relates to magnetic particle separators using micromachined magnetic arrays (52) deposited on to a substrate (50).

표면탄성파를 이용한 미세유동 크로마토 그래피 기반 미세입자 분리 장치 및 방법

본 발명은 표면탄성파를 이용한 미세유동 크로마토 그래피 기반 미세입자 분리 장치 및 방법에 관한 것이다. 본 발명은 압전기판; 압전기판에 패터닝되고, 압전기판으로 전기 에너지를 인가하여 표면탄성파를 발생시키는 한 쌍의 트랜스듀서; 압전기판에 설치되고, 트랜스듀서의 사이에 미세입자를 포함한 유체가 흐르는 미세유동 채널이 배치되는 미세유동칩; 및 미세유동 채널을 통과하면서 표면탄성파에 의해 분리된 미세입자를 검출하기 위한 검출부를 포함한다. 이와 같은 본 발명에 의하면, 유동하는 미세 입자에 유동저항을 발생시켜 입자의 크기 별로 이동 속도를 다르게하여 검출부에서 입자를 분리할 수 있고 검출 장치에 의하여 어떤 종류의 물질인지와 크기를 검출할 수 있다.

Magnetic bead-based arrays

The present invention relates to magnetic particle separators using micromachined magnetic arrays and more particularly, to magnetic particle separators or manipulators using controlled magnetization on micromachined magnetic arrays for the separation of cells and other biological materials. The present invention also pertains to using such devices for the separation and analysis of biological materials for immunoassays, DNA sequencing, protein analysis, and biochemical detection applications. The present invention can also be viewed as a novel method for fabricating fully integrated permanent magnet components within any microelectromechanical system (“MEMS”) structures. The present invention also provides a magnetic particle separation and manipulation system for rapid separation and accurate manipulation of magnetic particles in two-dimensional electromagnetic arrays, which utilize high throughput biological analyses. A disposable cartridge can be produced in low cost using a low cost substrate such as plastic or other polymer, glass, or metal. Magnetic flux is generated by conventional or micromachined electromagnets a platform system consisting of magnetic flux sources, magnetic flux guidance, and a microprocessor control interface. By controlling direction of electric currents into inductors on the platform system, arbitrary magnetic poles can be generated on Permalloy structures of the cartridge to separate and manipulate magnetic particles. The magnetic particle separator and manipulator in the present invention can be easily combined with automated detection systems such as a fluorescent monitoring system.

Magnetic bead-based arrays

The present invention relates to magnetic particle separators using micromachined magnetic arrays and more particularly, to magnetic particle separators or manipulators using controlled magnetization on micromachined magnetic arrays for the separation of cells and other biological materials. The present invention also pertains to using such devices for the separation and analysis of biological materials for immunoassays, DNA sequencing, protein analysis, and biochemical detection applications. The present invention can also be viewed as a novel method for fabricating fully integrated permanent magnet components within any microelectromechanical system (“MEMS”) structures. The present invention also provides a magnetic particle separation and manipulation system for rapid separation and accurate manipulation of magnetic particles in two-dimensional electromagnetic arrays, which utilize high throughput biological analyses. A disposable cartridge can be produced in low cost using a low cost substrate such as plastic or other polymer, glass, or metal. Magnetic flux is generated by conventional or micromachined electromagnets a platform system consisting of magnetic flux sources, magnetic flux guidance, and a microprocessor control interface. By controlling direction of electric currents into inductors on the platform system, arbitrary magnetic poles can be generated on Permalloy structures of the cartridge to separate and manipulate magnetic particles. The magnetic particle separator and manipulator in the present invention can be easily combined with automated detection systems such as a fluorescent monitoring system.