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Небесная энциклопедия

Космические корабли и станции, автоматические КА и методы их проектирования, бортовые комплексы управления, системы и средства жизнеобеспечения, особенности технологии производства ракетно-космических систем

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Мониторинг СМИ

Мониторинг СМИ и социальных сетей. Сканирование интернета, новостных сайтов, специализированных контентных площадок на базе мессенджеров. Гибкие настройки фильтров и первоначальных источников.

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Поддерживает ввод нескольких поисковых фраз (по одной на строку). При поиске обеспечивает поддержку морфологии русского и английского языка
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Применить Всего найдено 7148. Отображено 100.
02-02-2012 дата публикации

On-chip phase microscope/beam profiler based on differential interference contrast and/or surface plasmon assisted interference

Номер: US20120026509A1
Автор: Axel Scherer, Changhuei Yang, Demetri Psaltis, Xin Heng, Xiquan Cui
Принадлежит: California Institute of Technology CalTech

A differential interference contrast (DIC) determination device and method utilizes an illumination source, a layer having a pair of two apertures that receive illumination from the illumination source, and a photodetector to receive Young's interference from the illumination passing through the pair of two apertures. In addition, a surface wave assisted optofluidic microscope and method utilize an illumination source, a fluid channel having a layer with at least one aperture as a surface, and a photodetector that receives a signal based on the illumination passing through the aperture. The layer is corrugated (e.g., via fabrication) and parameters of the corrugation optimize the signal received on the photodetector.

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Номер записи: 1
01-03-2012 дата публикации

Laser microscope

Номер: US20120050733A1
Автор: Shinichi Takimoto
Принадлежит: Olympus Corp

Provided is a laser microscope, in which laser irradiation optical systems ( 3, 4, 5, 6 ) are employed for coaxially irradiating a sample with a CARS laser beam and a Raman scattering laser beam, and CARS light is detected by CARS light detecting means ( 12 ) and Raman scattering light is detected by Raman scattering light detecting means ( 13 ). In this manner, Raman scattering light observation and CARS light observation can be selectively performed without moving the sample, so that the vibration frequency for the CARS light observation can be efficiently selected without needing complicated work.

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Номер записи: 2
15-03-2012 дата публикации

Adaptor for microscopes

Номер: US20120062987A1
Автор: Brian J. HNATKOVICH, Christopher R. LITTEN, Craig Stout
Принадлежит: QBC DIAGNOSTICS Inc

A fluorescent microscope attachment is disclosed that includes a removable filter arm to provide an adaptor for use in transforming a light microscope into a fluorescent microscope. The adaptor may further include an LED light source and/or a magnetic microscope objective attachment.

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Номер записи: 3
22-03-2012 дата публикации

Method and apparatus for retrieval of amplitude and phase of nonlinear electromagnetic waves

Номер: US20120069345A1
Автор: Christian Depeursinge, Etienne Shaffer
Принадлежит: Ecole Polytechnique Federale de Lausanne EPFL

The present invention discloses a method and its associated apparatus to retrieve the amplitude and, especially, the phase of nonlinear electromagnetic waves. The application field of the present invention is optical imaging. A sample is probed by coherent electromagnetic radiation, and by a nonlinear interaction such as harmonic generation a nonlinear object wave is emitted. A nonlinear reference wave is generated by interaction of the same nature with the coherent electromagnetic radiation, and an interference between the nonlinear object wave and the nonlinear reference wave is sensed by a detector array. As an example, the technique makes possible real-time nanometric localization and tracking of nonlinear field emitters, such as, but not limited to, nanoparticles.

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Номер записи: 4
10-05-2012 дата публикации

Image processing apparatus, incubation observing apparatus, and image processing method

Номер: US20120114219A1
Автор: Hirotada WATANABE, Masato Nakagawa, Yoichi Wada
Принадлежит: KYOTO UNIVERSITY, Nikon Corp

An image processing apparatus includes an outline extracting processing unit inputting a phase contrast image of a cell colony acquired by an observing unit, and extracting an outline of the cell colony, an extracting unit extracting a feature quantity of an outline part of the cell colony based on brightness information at an outside and brightness information at an inside of the outline on the phase contrast image, and an automatic discriminating unit automatically discriminating whether or not the cell colony is an iPS cell colony based on a discriminant criterion determined in advance and the feature quantity extracted by the extracting unit.

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Номер записи: 5
31-05-2012 дата публикации

Method for imaging on thin solid-state interface between two fluids

Номер: US20120135410A1
Автор: Amit Meller, Gautam V. Soni
Принадлежит: Boston University

Described herein is a fluid cell for an optical microscopy tool having a solid state membrane having a first side and a second, opposing side; a first fluid chamber comprising a first fluid having a first refractive index located on the first side of the membrane; and, a second fluid chamber comprising a second fluid having a second refractive index located on the second side of the membrane, the second refractive index being different than the first refractive index. Also described herein is a method for imaging a single biomolecule, the method including generating a field of evanescent illumination at a solid state membrane between a first fluid and a second fluid having different refractive indexes; and detecting light emitted by optical detectors linked to the single biomolecules at the solid state membrane.

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Номер записи: 6
07-06-2012 дата публикации

Microscope, region determining method, and program

Номер: US20120140055A1
Автор: Goh Matsunobu, Ryu Narusawa
Принадлежит: Sony Corp

A microscope includes a dark-field illumination system that irradiates dark-field illumination light to a preparation in which a specimen is encapsulated between slide glass and cover glass by using an encapsulant, and an imaging unit that takes a dark-field image of the preparation irradiated with the dark-field illumination light. The microscope further includes a region determiner that detects the boundary between the encapsulant and air included between the slide glass and the cover glass based on the taken dark-field image and determines a region other than a region of the air as a region of interest for the specimen.

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Номер записи: 7
07-06-2012 дата публикации

Method for detection of specimen region, apparatus for detection of specimen region, and program for detection of specimen region

Номер: US20120140983A1
Автор: Goh Matsunobu, Koichiro Kishima, Ryu Narusawa
Принадлежит: Sony Corp

A method for detecting the specimen region includes the first step for the first region detecting unit to detect the first region which is a region with contrast in the first image of an object for observation which is photographed under illumination with visible light, the second step for the second region detecting unit to detect the second region which is a region with contrast in the second image of the object for observation which is photographed under illumination with ultraviolet light, and the third step for the specimen region defining unit to define, based on the first and second regions mentioned above, the specimen region where there exists the specimen in the object for observation.

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Номер записи: 8
09-08-2012 дата публикации

Transillumination device for a microscope

Номер: US20120200905A1
Автор: Didier Henry, Harald Schnitzler, Robert Paulus
Принадлежит: Leica Microsystems Schweiz AG

A transillumination device ( 150 ) for a microscope ( 100 ) comprises a flat panel light source ( 151 ), a diaphragm arrangement ( 152 ) arranged behind the flat panel light source ( 151 ) in the radiating direction (AR) that comprises two diaphragm elements movable relative to one another, at least one of the two diaphragm elements having a cutout, the two diaphragm elements defining, together with the at least one cutout, a diaphragm opening, wherein the dimensions of the diaphragm opening in two mutually perpendicular directions are determined by the position of the diaphragm elements relative to one another.

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Номер записи: 9
27-09-2012 дата публикации

System for and Method of Performing Multi-Technique Imaging

Номер: US20120241596A1
Автор: Cagri A. Savran, Khalid M. Arif
Принадлежит: PURDUE RESEARCH FOUNDATION

A system for and method of performing multi-technique imaging are disclosed. Such multi-technique imaging system includes a surface for supporting a specimen and at least two illumination sources for producing light radiation. The system also includes a plurality of reflective and refractive devices arranged to direct at least part of the light radiation from each of the at least two illumination sources to the surface such that the at least part of the light radiation from each of the at least two illumination sources illuminates substantially the same area on the surface. The system also includes a sensor configured to receive light radiation from the at least two illumination sources reflected by the specimen and/or that pass by the specimen. The system also includes a power source configured to power the at least two illumination sources and the sensor.

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Номер записи: 10
18-10-2012 дата публикации

Imaging apparatus and program

Номер: US20120262562A1
Автор: Naoki Fukutake, Shinichi Nakajima
Принадлежит: Nikon Corp

An imaging apparatus includes an imaging unit that images a test object, an analysis unit that outputs a feature amount of an image which is captured by the imaging unit, a storage unit that stores an evaluation function which has the image feature amount as a variable, for evaluation of the image, a selection unit that selects one image from two or more images including an image specified based on a value of the evaluation function, and a changing unit that changes the evaluation function based on the one image in a case where the one image selected by the selection unit is different from the specified image.

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Номер записи: 11
18-10-2012 дата публикации

Through-the-lens illuminator for optical comparator

Номер: US20120262707A1
Автор: David E. Lawson, Stephanie Bloch
Принадлежит: Quality Vision International Inc

An illumination system shares portions of an objective of an optical inspection system. A plurality of beam-shaping optics collects light from a plurality of effective light sources and directs the light through a portion of the objective for illuminating an object under inspection. The objective includes a front relay lens, a rear relay lens, and an objective stop disposed between the front and rear relay lenses for collecting light scattered from the object and forming an image of the object with the collected light. The beam-shaping optics, which surround the objective stop, are arranged together with the associated effective light sources for non-uniformly distributing light within a range of angles required for illuminating the object.

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Номер записи: 12
22-11-2012 дата публикации

Microscope system

Номер: US20120293644A1
Автор: Hiroki Yazawa, Naoki Fukutake, Shigeru Nakayama, Shinichi Nakajima
Принадлежит: Nikon Corp

A phase-contrast microscope system includes: an illumination optical system that illuminates a specimen with an illumination light from a light source; an imaging optical system that forms an image of the specimen from a light from the specimen; a first spatial modulation element that is disposed in a position of a pupil of the imaging optical system and changes an amplitude transmittance distribution of the light from the specimen; an image sensor that detects the image of the specimen by the imaging optical system and outputs a picture signal; a calculation section that calculates the amplitude transmittance distribution of the light from the specimen appropriate for observing the specimen on the basis of the output data detected by the image sensor and the amplitude transmittance distribution of the light from the specimen formed by the first spatial modulation element.

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Номер записи: 13
14-02-2013 дата публикации

Device and method for distributing illumination light and detected light in a microscope

Номер: US20130038875A1
Автор: Frank Schreiber
Принадлежит: Leica Microsystems CMS GmbH

A device for distributing illumination light and detected light in a microscope includes a distributor optic configured to guide illumination light onto a sample and guide detected light proceeding from the sample onto a detector. The distributor optic includes a polarization unit disposed in a first light path and configured to convert the illumination light directed onto the sample into a first polarization state, a beam splitter disposed in the first light path and having the polarization dependence so as to guide the converted illumination light onto the sample, a first portion of the detected light back into the first light path, and a second portion of the detected light into a second light path separated from the first light path. A beam combiner is configured to combine the first portion and the second portion of the detected light and guide the first portion and second portion onto the detector.

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Номер записи: 14
04-04-2013 дата публикации

Super-resolution observation aparatus

Номер: US20130083186A1
Автор: Shinichi Hayashi
Принадлежит: Olympus Corp

Super-resolution observation apparatus includes excitation light irradiation unit for irradiating excitation light intended to excite a sample on the sample, excitation light modulation unit for modulating a spatial intensity distribution of the excitation light on the sample, enlarged image forming unit for forming an enlarged image of the sample at an image position from observation light generated by irradiating the excitation light on the sample, image capturing unit for converting a spatial intensity distribution of the enlarged image into digital image data, and super-resolution processing unit for generating a super-resolution image where a super-resolution frequency component higher than a cutoff frequency of the enlarged image forming unit is made visible from one or a plurality of pieces of the digital image data. The super-resolution processing unit includes spatial frequency intensity modulation unit that change process contents according to an intensity level of a noise included in the digital image data.

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Номер записи: 15
18-04-2013 дата публикации

Omnidirectional super-resolution microscopy

Номер: US20130093871A1
Автор: Andreas G. Nowatzyk, Daniel FARKAS
Принадлежит: PHOTONANOSCOPY Inc

A microscopy method and apparatus includes placing a specimen to be observed adjacent to a reflective holographic optical element (RDOE). A beam of light that is at least partially coherent is focused on a region of the specimen. The beam forward propagates through the specimen and is at least partially reflected backward through the specimen. The backward reflected light interferes with the forward propagating light to provide a three dimensional interference pattern that is at least partially within the specimen. A specimen region illuminated by the interference pattern is imaged at an image detector. Computational reconstruction is used to generate a microscopic image in all three spatial dimensions (X,Y,Z), simultaneously with resolution greater than conventional microscopy.

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Номер записи: 16
18-04-2013 дата публикации

Fast, modular port switcher for an optical microscope using a galvanometer

Номер: US20130094071A1
Автор: Glen Ivan Redford
Принадлежит: Intelligent Imaging Innovations Inc

A fast modular port switching device is described. The device can be used with an optical microscope to facilitate using multiple devices with the microscope. The port switching is done with a galvanometer for switching very fast. The device is modular so it can be combined with any number of similar devices for building a complex, multi-modal imaging system. Also described is the combination of a port switcher with automated spherical aberration correction. Even further described is a similar device where the outputs are recombined, thus making the device a fast filter switcher.

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Номер записи: 17
02-05-2013 дата публикации

Method and system for illuminating a sample

Номер: US20130107358A1
Автор: Frank Sieckmann, Werner Knebel
Принадлежит: Leica Microsystems CMS GmbH

A method for illuminating at least one sample in SPIM microscopy includes generating a light beam and forming a light strip from the light beam using an optical device that interacts with the light beam. The light strip is passed strip through at least one objective having optics configured to deliver detection light emanating from the sample directly or indirectly to a detector, with the objective optics interacting with the light strip. The light strip is deflected using a light-redirecting device downstream of the objective optics so as to propagate the light strip, after deflection, at an angle other than zero degrees with respect to an optical axis of the objective in order to illuminate the sample.

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Номер записи: 18
20-06-2013 дата публикации

High Resolution Microscope and Image Divider Assembly

Номер: US20130155218A1
Автор: Goelles Michael, Kalkbrenner Dr. Thomas
Принадлежит:

The invention relates to a microscope having an illumination beam path with wide field illumination of a sample and a first detection beam path having a spatially resolved surface receiver, which is reached by a first part of the detection light coming from the sample via the first detection beam path, or an image divider assembly for a microscope. In order to lengthen the optical path length, at least a second part of the detection light coming from the sample is masked out of the detection beam path and, via deflection means belonging to the detection beam path, is led into a second detection beam path and, preferably via further deflection means, is deflected back in the direction of the detection in such a way that detection light is applied to at least two partial regions beside one another on the surface receiver. At least the second part of the detection light runs in an optical element having an optical density that is increased as compared with the first detection beam path, in order to lengthen the optical path length, and the optical element is designed to be displaceable at an angle, preferably perpendicular, to the optical axis of the first detection beam path in order to adjust the optical path length, and has flat surfaces, at least on the light entry and light exit side thereof; a prism is provided, preferably a glass prism, preferably at least in the second detection beam path after a first beam deflection, for deflection in a direction parallel to the first detection beam path, in order to increase the path length and for reverse deflection. 1. A microscope having an illumination beam path with wide-field illumination of a sample and a first detection-beam path that includes a spatially resolved surface receiver , that is reached by a first part of the detection light coming from the sample via the first detection-beam path or an image splitter arrangement for a microscope , and at least a second part of the detection light coming from the sample ...

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Номер записи: 19
27-06-2013 дата публикации

Quantitative phase microscopy for label-free high-contrast cell imaging using frequency domain phase shift

Номер: US20130162800A1
Автор: Dmitry Vladimirovich Dylov, Evgenia Mikhailovna Kim, William Albert Challener
Принадлежит: General Electric Co

Some systems described herein include a frequency dependent phase plate for generating multiple phase-contrast images of a sample, each from a different frequency range of light, each phase-contrast image for frequency range of light formed from light diffracted by the sample interfered with undiffracted light that has a frequency-dependent baseline relative phase shift from the phase plate. In some embodiments, the multiple phase-contrast images may be used to generate a quantitative phase image of a sample. The phase-contrast images or the produced quantitative phase image may have sufficient contrast for label-free auto-segmentation of cell bodies and nuclei.

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Номер записи: 20
04-07-2013 дата публикации

Convex Lens-Induced Confinement for Measuring Distributions of Molecular Size

Номер: US20130170026A1
Автор: Adam E. Cohen, Sabrina R. Leslie
Принадлежит: Individual

A curved surface is placed tangent to a slide and displaces a sample liquid from the point or line of contact outward. Imaging indicates a region where fluorescence is observed, and the location of the fluorescence indicates the molecular size. The radius of curvature of the lens is known, the distance from the (center) point of contact of the observed fluorescence is measured with a microscope and the distance of the lens surface to the slide's surface can then be calculated. This distance represents the size of the molecule or ensemble of molecules emitting. Similarly, absorbance, etc. could be measured with a light source below the slide.

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Номер записи: 21
25-07-2013 дата публикации

Microscope and inspection apparatus

Номер: US20130188251A1
Автор: Haruhiko Kusunose, Takao Tsubouchi
Принадлежит: Lasertec Corp

A system including a microscope and an inspection apparatus in which an objective lens having a large numerical aperture is used for detecting a defect existing inside a sample. A light source apparatus produces linearly polarized light. The polarization maintaining fibers optically coupled to the light source apparatus project the linearly polarized light onto the sample surface as an illumination beam of P-polarized light at an incidence angle substantially equal to the Brewster's angle of the sample. The scattered light generated by the defect existing in the sample is emitted from the sample and is collected by the objective lens whose optical axis is perpendicular to the sample surface. Since the illumination beam of P-polarized light is projected at the incidence angle equal to the Brewster's angle of the sample, no surface reflection occurs and it is possible to use the objective lens having a large numerical aperture.

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Номер записи: 22
05-09-2013 дата публикации

Microscopic device and microscopic method for the three-dimensional localization of point-like objects

Номер: US20130229494A1
Автор: Marcus Dyba
Принадлежит: Leica Microsystems CMS GmbH

A microscopic device provides three-dimensional localization of point-like objects and includes two imaging optics, each configured to image a same point-like object located in an object space into two separate image spaces as a focused light distribution. Two detector units are respectively associated with the imaging optics and configured to capture an analyzable light spot in detection points of a detection surface disposed in the respective image space. Each imaging optics includes an optical device that orients the focused light distributions obliquely to a detection axis such that, taking into account the detection point correspondence, the two light spots shift in opposite directions based on a z-position of the point-like object. An evaluation unit brings the detection points of the two detection surfaces into mutual pairwise correspondence and analyzes the two light spots so as to ascertain a lateral x-y position and an axial z-position of the point-like object.

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Номер записи: 23
05-09-2013 дата публикации

Microscope Having A Switchable Documentation Beam Path

Номер: US20130229626A1
Автор: Wenk Urban, ZUEND René
Принадлежит: Leica Microsystems (Schweiz) AG

A microscope () having a principal observer's beam path () and an assistant's beam path (3), and having an optical beam splitter device () for generating a documentation beam path (), where the beam splitter device () in a first position outcouples the documentation beam path from the principal observer's beam path (), and in a second position outcouples it from the assistant's beam path (). 11. A microscope () , comprising:{'b': '2', 'i': 'a', "a principal observer's beam path ();"}{'b': 3', '3, 'i': a,', 'b, "an assistant's beam path (); and"}{'b': 5', '15', '4, 'an optical beam splitter device (, ) for generating a documentation beam path ();'}{'b': 5', '15', '2', '3, 'i': a', 'b, "wherein, in a first position, the optical beam splitter device (, ) is configured to outcouple the documentation beam path from the principal observer's beam path (), and in a second position configured to outcouple the documentation beam path from the assistant's beam path ()."}2332787a,ba. The microscope according to claim 1 , wherein the assistant's beam path () and the principal observer's beam path () extend from a microscope objective () separately from one another claim 1 , parallel to the main axis () of the microscope objective ().3251542aa. The microscope according to claim 1 , wherein the principal observer's beam path includes two stereoscopic beam paths () claim 1 , wherein in the first position of the beam splitter device ( claim 1 , ) the documentation beam path () is configured to be outcoupled from one of the two principal observer's beam paths ().43351543a,bb. The microscope according to claim 1 , wherein the assistant's beam path includes two stereoscopic assistant's beam paths () claim 1 , wherein in the second position of the beam splitter device ( claim 1 , ) the documentation beam path () is configured to be outcoupled from one of the two assistant's beam paths ().554623ab. The microscope according claim 1 , wherein the beam splitter device () includes claim 1 , ...

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Номер записи: 24
19-09-2013 дата публикации

Microscope

Номер: US20130242078A1
Автор: Kazumi Yokota, Takeshi Maji, Tomoki Sasayama
Принадлежит: Shimadzu Corp

A microscope is provided. The microscope includes: a detection section for detecting the measurement light; a first image acquisition section emitting the visible light onto a detection surface to obtain an optical image; and a switch mirror or beam splitter disposed on a light path, along which the measurement light from the analysis position of the sample is guided to the detection section. The microscope further includes a second image acquisition section that is disposed in a position apart from the light path of the detection section for obtaining an optical image of a large area which includes the analysis position of the sample, wherein the optical image of the large area is larger than an optical image of an area, which includes the analysis position of the sample, obtained by the first image acquisition section.

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Номер записи: 25
19-09-2013 дата публикации

Microscope system, driving method of the same, and computer-readable recording medium

Номер: US20130242382A1
Автор: Toshiya Komuro
Принадлежит: Olympus Corp

A microscope system includes a plurality of actuators configured to cause components of a microscope to operate, a plurality of power drivers configured to drive the plurality of actuators, respectively, an actuator controller configured to control the plurality of actuators through the plurality of power drivers, and a control management unit configured to control the actuator controller to designate any one of the plurality of power drivers to output a start signal for allowing the designated power driver to start operation to the designated power driver, and to control the actuator controller to stop outputting of the start signal to the designated power driver and the other power drivers other than the designated power driver until passage of a settling time in which driving of one of the plurality of actuators in response to the operation of the designated power driver is completed and then enabled again.

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Номер записи: 26
03-10-2013 дата публикации

Light microscope and method for recording images with a light microscope

Номер: US20130258090A1
Автор: Cornelia Bendlin, Matthias Langhorst, Peter Schoen, Ralf Steinmeyer
Принадлежит: CARL ZEISS MICROSCOPY GMBH

The invention relates to a method for recording images with a light microscope, wherein a specimen container with a specimen is arranged on a specimen holder of the light microscope, and wherein illuminating light is guided onto the specimen. The illuminating light can hereby be cut in a cross-section transversely to an optical axis of the light microscope through a wall of the specimen container to a limited cross-sectional region. First and second diaphragm settings are determined and set, for the limited cross-sectional region of the illuminating light defined by the wall of the specimen container, in which the diaphragm covers equal sized portions of the limited cross-sectional region. In addition the invention relates to a light microscope which is adapted in particular to carry out the method.

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Номер записи: 27
24-10-2013 дата публикации

INTERFEROMETRIC SYSTEMS AND METHODS

Номер: US20130278922A1
Автор: Gelernt Barry, Jota Thiago, Milster Thomas D.
Принадлежит: INVENT TECHNOLOGIES, LLC

Optical systems and methods including interferometric systems and methods are disclosed herein. In some embodiments, the present invention relates to a system comprising at least one light source including a deep ultraviolet light source, a lens device, a beam splitter, and a camera device. The lens device receives first light, directs at least some of that light toward a target location, receives reflected light therefrom, and directs at least some of the reflected light toward a further location, where at least part of a light path between the deep ultraviolet light source and the target location is other than at a high vacuum. The camera device is positioned at either the further location or an additional location, whereby an image is generated by the camera device based upon at least a portion of the reflected light. Also encompassed herein are interferometric lithography and optical microscopy systems. 1. An optical system comprising:at least one light source including a deep ultraviolet light source configured to generate first light having a wavelength within a window in the deep ultraviolet region of the electromagnetic spectrum within which a local minimum in the absorption coefficient of oxygen occurs, wherein the wavelength is approximately 121.6 nm, and wherein the at least one light source is further configured to generate second light that includes visible light or near-ultraviolet light and that is directed toward a target location;a lens device that receives at least a first portion of the generated first light, directs at least some of the first portion of the generated first light toward the target location, receives reflected light from the target location, and directs at least some of the reflected light toward a further location, wherein at least a part of a light path between the deep ultraviolet light source and the target location is other than at a high vacuum;a beam splitter positioned between at least two of the deep ultraviolet light ...

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Номер записи: 28
14-11-2013 дата публикации

Increased depth-resolution microscopy

Номер: US20130302905A1
Автор: Helmut Lippert, Ingo Kleppe, Thomas Kalkbrenner
Принадлежит: CARL ZEISS MICROSCOPY GMBH

A method for high-resolution luminescence microscopy of a sample marked with marking molecules that can be activated to excite particular luminescent radiation, including: repeated activation of a subset of the marking molecules to emit luminescent radiation; repeated imaging of the sample along a depth direction and with a predetermined optical resolution; and producing images from the repeated imaging. Locations of the marking molecules are determined with a spatial resolution that is increased above the predetermined optical resolution. Activation of the marking molecules can be through radiation introduced into multiple regions, each extending along a plane substantially perpendicular to the depth direction. The regions can be arranged so that the regions are behind one another and overlap only partially. Separate images of the sample may be recorded for activation in each of the regions in order to obtain depth information relating to the marking molecules from the separate images.

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Номер записи: 29
28-11-2013 дата публикации

System and method of multitechnique imaging for the chemical biological or biochemical analysis of a sample

Номер: US20130314528A1
Автор: Anne Chantal Gouget Laemmel, Elisabeth Anne-Gabrielle Galopin, Francois Ozanam, Jean-Noel Chazalviel, Larbi Touahir, Rabah Boukherroub, Sabine Szunerits
Принадлежит: CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS, ECOLE POLYTECHNIQUE, Universite De Lille 1 Sciences Et Technologies

The invention substantially relates to an imaging system for the chemical, biological or biochemical analysis of a sample ( 10 ), comprising a device ( 40 ) for holding the sample ( 10 ), a device ( 20 ) for optical detection, a lighting device ( 30 ), liable to emit a monochromatic light beam, and a wide aperture objective (f 1 ), It is substantially characterized in that the objective (f 1 ) is configured for, downstream of the sample ( 10 ): focusing the excitation light beam reflected at a point (C) located in the focal plane (FF) of said objective (f 1 ), and transforming the retransmitted or scattered light beam into a quasi-parallel beam, and in that the system further comprises a device ( 70 ) for selectively blocking the collected light beam by said objective (f 1 ).

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Номер записи: 30
28-11-2013 дата публикации

Microscope apparatus

Номер: US20130314775A1
Автор: Nobuhiro Takamizawa
Принадлежит: Olympus Corp

This microscope apparatus is provided with the following elements: an objective lens; a CCD that constructs an image of a sample S; an illumination intensity change unit for adjusting an intensity of excitation light; an sensitivity adjustment unit for adjusting a detection sensitivity by the CCD; a galvanometer mirror for changing a light focusing position of excitation light at a pupil position of the objective lens; a storage unit that stores, for each observation method, a predetermined intensity, a predetermined detection sensitivity, and the light focusing position; and a control unit that switches the observation method, reads out the intensity, the detection sensitivity, and the light focusing position according to the observation method based on a synchronization signal synchronized with a frame signal for constructing an image, and controls the illumination intensity change unit, the sensitivity adjustment unit, and the galvanometer mirror.

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Номер записи: 31
19-12-2013 дата публикации

DARK FIELD ILLUMINATION METHOD AND A DARK FIELD ILLUMINATOR

Номер: US20130335976A1
Автор: BEN-EZER ZEHAVA
Принадлежит:

A dark field illuminator that includes a light source adapted to provide a ring of light characterized by uniform intensity distribution; a collimating ring adapted to receive the ring of light and to direct collimated light beams towards an area of an inspected object such that different points within the area are illuminated by light beams that form substantially identical cones of light; and wherein the collimating ring and the light source are co-centric to an optical axis of the dark field illuminator. 1. A dark field illuminator , comprising: a light source adapted to provide a ring of light characterized by uniform intensity distribution; a collimating ring adapted to receive the ring of light and to direct collimated light beams towards an area of an inspected object such that different points within the area are illuminated by light beams that form substantially identical cones of light; and wherein the collimating ring and the light source are co-centric to an optical axis of the dark field illuminator.2. The dark field illuminator according to wherein the light source has an opening through which at least one lens of an imaging optics can be placed.3. The dark field illuminator according to wherein the light source is a pulsed light source.4. The dark field illuminator according to wherein the light source is a continuous light source.5. A dark field illuminator claim 1 , comprising: a light source adapted to provide a ring of light characterized by uniform intensity distribution; a folding ring reflector adapted to receive the ring of light and to direct reflected light beams towards a collimating ring; a collimating ring adapted to receive the reflected light beams and to direct collimated light beams towards an area of an inspected object such that different points within the area are illuminated by identical cones of light; wherein the collimating ring claim 1 , the ring reflector and the light source are co-centric to an optical axis of the dark ...

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Номер записи: 32
26-12-2013 дата публикации

Variable orientation illumination-pattern rotator

Номер: US20130342886A1
Автор: Jeremy R. COOPER
Принадлежит: Applied Precision Inc

Variable orientation illumination-pattern rotators (“IPRs”) that can be incorporated into structured illumination microscopy instruments to rapidly rotate an interference pattern are disclosed. An IPR includes a rotation selector and at least one mirror cluster. The rotation selector directs beams of light into each one of the mirror clusters for a brief period of time. Each mirror cluster imparts a particular predetermined angle of rotation on the beams. As a result, the beams output from the IPR are rotated through each of the rotation angles imparted by each of the mirror clusters. The rotation selector enables the IPR to rotate the beams through each predetermined rotation angle on the order of 5 milliseconds or faster.

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Номер записи: 33
30-01-2014 дата публикации

Optical element, optical device, measurement device, and screening apparatus

Номер: US20140027653A1
Автор: Kunihiko Yoshino, Masanobu Kato, Muneki Hamashima, Susumu Mori, Takehiko Ueda, Tomoya Saito
Принадлежит: Nikon Corp

An optical element includes a separation section that can separate incident light according to a wavelength. The separation section has an optical characteristic in which incident light in a first wavelength band is reflected, incident light in a second wavelength band is transmitted, and incident light in a third wavelength band is partially transmitted and partially reflected.

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Номер записи: 34
13-02-2014 дата публикации

Microscope with a viewing direction perpendicular to the illumination direction

Номер: US20140042339A1
Автор: Ernst H.K. Stelzer, James H. Swoger, Jan Huisken, Sebastian Enders, Steffen Lindek
Принадлежит: Europaisches Laboratorium fuer Molekularbiologie EMBL

A microscope and imaging method in which a layer of the sample is illuminated by a thin strip of light and the sample is viewed perpendicular to the plane of the strip of light. The depth of the strip of light thus essentially determines the depth of focus of the system. To record the image, the object is displaced through the strip of light, which remains fixed in relation to the detector, and fluorescent and/or diffused light is captured by a planar detector. Objects that absorb or diffuse a large amount of light are viewed from several spatial directions. The three-dimensional images, which are captured from each direction can be combined retrospectively to form one image, in which the data is weighted according to its resolution. The resolution of the combined image is then dominated by the lateral resolution of the individual images.

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Номер записи: 35
20-02-2014 дата публикации

Fluorescence observation device, domed base and fluorescence microscope provided with multiple light sources having different illumination angles

Номер: US20140049817A1
Автор: Chih-Yi Yang
Принадлежит: Lumos Technology Co Ltd

The present invention relates to a fluorescence observation device, a domed base and a fluorescence microscope. The fluorescence observation device includes a dome, a multi-angle light-emitting unit and a controller unit. The dome includes a transparent thermal insulation layer and a heat sink layer. The multi-angle light-emitting unit includes multiple directional narrow light field electro-luminescence devices thermally connected to the heat sink layer and adapted to emit light at different angles to pass through the transparent thermal insulation layer. The narrow light field electro-luminescence devices are selectively activated to emit light by the controller unit, so that the illumination angle of the multi-angle light-emitting unit can be adjusted. The fluorescence observation device can be combined with a base to constitute the domed base. Alternatively, narrow light field electro-luminescence devices are mounted on the base.

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Номер записи: 36
27-02-2014 дата публикации

Laser scanning microscope

Номер: US20140055852A1
Автор: E. Szilveszter Vizi, Gergely Katona, J. Balázs RÓZSA
Принадлежит: FEMTONICS KFT

A laser scanning reflection or fluorescent microscope is provided with focusing-detecting unit having a laser beam focusing objective, an image detector that detects light reflected from the sample or back fluoresced light emitted by the sample, and a drive that simultaneously displaces the objective and the image detector.

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Номер записи: 37
06-03-2014 дата публикации

Multiple wavelength led array illuminator for fluorescence microscopy

Номер: US20140063797A1
Автор: Jeffrey B. Lee, Junying Jonathan Lu, Robert E. Schleicher
Принадлежит: Dicon Fiberoptics Inc

One embodiment provides light along an optical axis. It comprises a substrate and at least one array of multiple LED chips without individual packaging supported by the substrate. The LED chips emit light within different wavelength ranges and are distributed laterally with respect to the axis over an area, the LED chips having light emitting surfaces for emitting light in directions transverse to the area. An optical element adjacent to the light emitting surfaces of the LED chips in the at least one array collects and directs light emitted by the LED chips of the at least one array along the axis towards a target. Another embodiment is directed to a method for providing multiple wavelength light for fluorescent microscopy using the above system. Electric current is supplied to the multiple LED chips, causing them to emit light of multiple wavelengths. The currents supplied to the multiple LED chips are controlled so as to control the exposure of fluorescent dyes with different excitation wavelengths wherein the light emitted by the multiple LED chips include wavelength components at such different excitation wavelengths without having to move the multiple LED chips.

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Номер записи: 38
01-01-2015 дата публикации

MICROSCOPE AND METHOD FOR HIGH-RESOLUTION 3-D FLUORESCENCE MICROSCOPY

Номер: US20150002632A1
Автор: Kalkbrenner Thomas, Wolleschensky Ralf
Принадлежит:

In a sample, fluorescence emitters are repeatedly excited to emit fluorescence, and still images are produced of the sample by means of a microscope. At least a subset of the fluorescence emitters is isolated in each still image. The positions of the fluorescence emitters are localized in the still images with a location accuracy exceeding the optical resolution. A high-resolution composite image is generated therefrom. An adaptive mirror is arranged in the imaging beam path, and is adjusted in such a manner that it produces an astigmatism when at least one of the still images is produced. As a result, still images with astigmatism are captured. Depth position information for the fluorescence emitters is derived from the rotational asymmetry. The adaptive mirror is additionally adjusted in such a manner that it does not produce any astigmatism when some of the still images are produced. 1. A method for high-resolution 3D fluorescence microscopy , comprising(a) repeatedly exciting fluorescence emitters in a sample to emit fluorescence, and producing still images of the sample by means of a microscope having an imaging beam path with an optical resolution and a focal plane, said fluorescence emitters being stimulated to emit fluorescence in such a manner that at least a subset of the fluorescence emitters is isolated in each still image, so that the images of these fluorescence emitters can be separated in the still images within the optical resolution,(b) localizing the positions of the fluorescence emitters from the images of the isolated fluorescence emitters in the resulting still images, with a location accuracy exceeding the optical resolution, and generating a high-resolution composite image therefrom,(c) arranging an adaptive mirror in the imaging beam path of the microscope, and adjusting said mirror in such a manner that it produces an astigmatism when at least some of the still images are produced, thereby capturing still images with astigmatism, said ...

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Номер записи: 39
05-01-2017 дата публикации

NANOMETER SCALE MICROSCOPY VIA GRAPHENE PLASMONS

Номер: US20170003223A1
Автор: Al-Amri Mohammad D., Zeng Xiaodong, Zubairy Muhammad Suhail
Принадлежит:

Novel and advantageous systems and methods for performing nanometer-scale microscopy using graphene plasmons (GPs) are provided. Sub-diffraction microscopy can be achieved, taking advantage of the extremely small plasmon wavelength and low dissipation of GPs. Nanometer-scale resolution can be obtained under very weak light intensity, which is especially important in the imaging of biological systems. 1. A method of performing nanometer-scale microscopy on a sample , the method comprising:i) providing the sample to an imaging system comprising a graphene layer, wherein the sample is disposed over the graphene layer;ii) exciting a plurality of graphene plasmons (GPs) in the graphene layer as an illumination source for imaging the sample; andiii) capturing an image of the sample using the GPs as the illumination source.2. The method according to claim 1 , wherein the imaging system further comprises a dielectric layer disposed on the graphene layer claim 1 , such that the sample is disposed on the dielectric layer.3. The method according to claim 2 , wherein the imaging system further comprises a lens claim 2 ,wherein the sample is disposed between the graphene layer and the lens, andwherein capturing the image of the sample is performed by the lens.4. The method according to claim 2 , wherein the dielectric layer has a thickness that is set or tuned to optimize the imaging result based on a desired result.5. The method according to claim 2 , wherein the graphene layer is in direct physical contact with a lower surface of the dielectric layer claim 2 , andwherein, during the steps of exciting the plurality of GPs and capturing the image of the sample, the sample is in direct physical contact with an upper surface of the dielectric layer.6. The method according to claim 3 , wherein the imaging system further comprises a substrate on which the graphene layer is disposed claim 3 ,wherein the substrate comprises dielectric gratings therein,wherein the dielectric gratings ...

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Номер записи: 40
05-01-2017 дата публикации

COMPRESSIVE PLENOPTIC MICROSCOPY

Номер: US20170003491A1
Автор: Adesnik Hillel, Pegard Nicolas, Waller Laura
Принадлежит: The Regents of the University of California

A system and method for quantitative functional neuroimaging through thick brain tissue in live animals. A computational imaging method is disclosed that uses plenoptic image acquisition including a first initialization step that identifies individual neurons by their optical signature and provides a reliable estimate of their position in space and a second stimulation-based image processing step that used acquired calibration data to quickly quantify activity in each identified neuron at video frame-rate. 1. A functional imaging apparatus , comprising:(a) a computer processor; and(b) a memory storing instructions executable by the computer processor; (i) acquiring initial image data from a sample tissue, the initial image data comprising features corresponding to a plurality of neurons within the sample tissue;', '(ii) generating a database of individual optical signatures, each of the individual optical signatures corresponding to an identified neuron within the plurality of neurons;', '(iii) acquiring secondary image data from the sample tissue, the secondary image data comprising features corresponding to a plurality of neurons responsive to an external stimulus applied to the sample tissue; and', '(iv) decomposing the secondary image data as a function of the database of individual optical signatures to output a quantitative measure of fluorescence levels of the identified individual neurons., '(c) said instructions, when executed by the computer processor, performing steps comprising2. The apparatus of claim 1 , wherein the database comprises data corresponding to an estimated location in space for each identified neuron within the plurality of neurons.3. The apparatus of claim 1 , wherein the secondary image data is decomposed as a function of a positive claim 1 , linear combination of one or more image frames within database of individual optical signatures.4. The apparatus of :wherein acquiring initial image data comprises acquiring video data from the ...

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Номер записи: 41
05-01-2017 дата публикации

FULLY AUTOMATIC RAPID MICROSCOPE SLIDE SCANNER

Номер: US20170003492A1
Автор: Soenksen Dirk G.
Принадлежит:

Microscope slide scanner. In an embodiment the microscope slide scanner comprises a single enclosure unit that includes at least one objective lens, at least one line scan camera, at least one communication port, and at least one processor. The line scan camera may be configured to capture image data of a sample as a plurality of image stripes via the objective lens. The communication port provides communication over a network. The processor may align the plurality of image stripes into a contiguous image of at least a portion of the sample, and executes a web server that provides an operator interface over the network to one or more remote devices. 1a stage configured to support a microscope slide;an objective lens positioned to view a portion of the microscope slide;a line scan camera optically coupled with the objective lens, the line scan camera configured to create a digital image strip of a portion of the microscope slide;a processor configured to align adjacent digital image strips into a contiguous digital image; anda data storage area configured to store the contiguous digital image.. A microscope slide scanner comprising: The present application is a continuation of U.S. patent application Ser. No. 14/275,021, filed May 12, 2014, which is a continuation of U.S. patent appllication Ser. No. 13/771,831, filed Feb. 20, 2013, and issued as U.S. Pat. No. 8,755,579, which is a continuation of U.S. patent application Ser. No. 13/291,971, filed Nov. 8, 2011 and issued as U.S. Pat. No. 8,385,619, which is a continuation of U.S. patent application Ser. No. 12/344,037, filed Dec. 23, 2008 and issued as U.S. Pat. No. 8,055,042, which is a continuation of U.S. patent application Ser. No. 12/235,479, filed Sep. 22, 2008 and issued as U.S. Pat. No. 7,826,649, which is a continuation of U.S. patent application Ser. No. 11/379,648, filed Apr. 21, 2006 and issued as U.S. Pat. No. 7,428,324, which is a continuation of U.S. patent application Ser. No. 10/371,586, filed Feb. ...

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Номер записи: 42
07-01-2016 дата публикации

DIFFERENTIAL SCAN IMAGING SYSTEMS AND METHODS

Номер: US20160003739A1
Автор: Bouzid Ahmed
Принадлежит:

Systems and methods for producing background-reduced fluorescence imaging signals include an illumination system that provides illumination light from an illumination source to a targeted area on the sample platform, a sensor adapted to detect light and having an array of sensing locations, and collection optics arranged and configured to project light emanating from the sample platform onto the sensor. In typical operation, light from the targeted area is projected onto a first portion of the sensor comprising a first plurality of the sensing locations and light from proximal to the targeted area on the platform is projected onto a second portion of the sensor comprising a second plurality of the sensing locations, and a second signal detected by the second portion of the sensor is subtracted from a first signal detected by the first portion of the sensor to produce a background-reduced signal, e.g., a signal with reduced background related noise. 1. An imaging system , comprising:a sample platform; and an illumination system that provides illumination light from an illumination source to a targeted area on the sample platform;', 'a sensor adapted to detect light and having an array of sensing locations; and', 'collection optics arranged and configured to project light emanating from the sample platform onto the sensor,, 'an illumination and detection system, includingwherein light from the targeted area is projected onto a first portion of the sensor comprising a first plurality of the sensing locations and light from proximal to the targeted area on the platform is projected onto a second portion of the sensor comprising a second plurality of the sensing locations, andwherein a second signal detected by the second portion of the sensor is subtracted from a first signal detected by the first portion of the sensor to produce a background-reduced signal.2. The system of claim 1 , wherein the system is a fluorescence imaging system claim 1 , wherein the targeted area ...

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Номер записи: 43
07-01-2016 дата публикации

APPARATUS AND METHODS FOR FLUORESCENCE IMAGING USING RADIOFREQUENCY-MULTIPLEXED EXCITATION

Номер: US20160003741A1
Автор: Buckley Brandon, Diebold Eric D., Jalali Bahram
Принадлежит: The Regents of the University of California

Apparatus and methods for fluorescence imaging using radiofrequency multiplexed excitation. One apparatus splits an excitation laser beam into two arms of a Mach-Zehnder interferometer. The light in the first beam is frequency shifted by an acousto-optic deflector, which is driven by a phase-engineered radiofrequency comb designed to minimize peak-to-average power ratio. This RF comb generates multiple deflected optical beams possessing a range of output angles and frequency shifts. The second beam is shifted in frequency using an acousto-optic frequency shifter. After combining at a second beam splitter, the two beams are focused to a line on the sample using a conventional laser scanning microscope lens system. The acousto-optic deflectors frequency-encode the simultaneous excitation of an entire row of pixels, which enables detection and de-multiplexing of fluorescence images using a single photomultiplier tube and digital phase-coherent signal recovery techniques. 1. An apparatus for optical interrogation of a sample , comprising: (1) a laser light source;', '(2) an acousto-optic deflector; and', '(3) a radio frequency comb generator;, '(a) a first beam generator, comprising (1) a laser light source;', '(2) an acousto-optic frequency shifter; and', '(3) a radio frequency tone generator;, '(b) a second beam generator, comprising(c) an objective lens system configured to expose a sample to beams from said first and second beam generators over time; and(d) a photodetector;wherein light emissions from the sample from exposure to beams from the first and second beam generators are detected by the photodetector.2. The apparatus as recited in claim 1 , wherein said objective lens system comprises:a scanning mirror;a scan lens;a tube lens; andan objective lens.3. The apparatus as recited in claim 1 , wherein said photodetector comprises:a fluorescence emission filter;a slit aperture; anda photo-multiplier tube.4. The apparatus as recited in claim 3 , said photodetector ...

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Номер записи: 44
01-01-2015 дата публикации

SYSTEMS AND METHODS FOR IMAGING AT HIGH SPATIAL AND/OR TEMPORAL PRECISION

Номер: US20150004637A1
Автор: Cohen Adam E., Hochbaum Daniel, Kralj Joel, Maclaurin Dougal
Принадлежит:

Various aspects of the present invention are generally directed to systems and methods for imaging at high spatial and/or temporal resolutions. In one aspect, the present invention is generally directed to an optical microscopy system and related methods adapted for high spatial and temporal resolution of dynamic processes. The system may be used in conjunction with fluorescence imaging wherein the fluorescence may be mediated by voltage-indicating proteins. In some cases, time resolutions may be enhanced by fitting predefined temporal waveforms to signal values received from an image. The system may also contain a high numerical aperture objective lens and a zoom lens located in an imaging optical path to an object region. Other aspects of the present invention are generally directed to techniques of making or using such systems, kits involving such systems, manufactured storage devices able to implement such systems or methods, and the like. 1. A method for temporally resolving a time-varying image , the method comprising:receiving, from a plurality of imaging pixels, a plurality of signal values associated with a plurality of measurement time bins during which the time-varying image was obtained; andfitting, for at least some of the pixels, a pre-defined temporal waveform to the respective signal values received for each pixel.2. The method of claim 1 , wherein the fitting provides a temporal resolution finer than the smallest duration of any of the measurement time bins.3. The method of any one of or claim 1 , wherein the time-varying image is obtained with a microscope.43. The method of any one of - claims 1 , wherein the time-varying image is obtained with a fluorescence microscope.5. The method of any one of or claims 1 , wherein the time-varying image is obtained with an X-ray imaging system.6. The method of any one of or claims 1 , wherein the time-varying image is obtained with a magnetic resonance imaging system.76. The method of any one of - claims 1 , ...

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Номер записи: 45
07-01-2016 дата публикации

PORTABLE MICROSCOPE DEVICE

Номер: US20160004057A1
Автор: Chen Chang-Yu, CHIANG Tsun-Chao, LIN Cheng-Ming, LIN Shu-Sheng
Принадлежит:

The present invention discloses a portable microscope device which can be installed on the smartphone capable of capturing image. By combing these devices, users can observe the detection sample and capture the image of the sample instantly without environment limitation. Moreover, during operation, the user can observe the whole image of the sample by substituting the microscope lens of different magnification ratio or by shifting the position of the sample. 1. A portable microscope device , for cooperating with a communication device capable of capturing image and utilizing an image capture module of the communication device to capture an image of a detection sample , comprising:a base, having an illumination module;a cover, movably installed on the base;a microscope module, having a microscope lens movably installed on the cover to enable the image capture module of the communication device to capture the image of the detection sample by the microscope lens.2. The portable microscope device of claim 1 , wherein a magnetic attraction portion is installed on the periphery of the detective portion claim 1 , a relative magnetic attraction portion of the microscope module is attracted to the magnetic attraction portion to enable the microscope module and the cover to relatively move under the condition that they are not separated.3. The portable microscope device of claim 1 , wherein the base has a restrictive groove on an upper plane thereof for a microscope slide disposed in the restrictive groove.4. The portable microscope device of claim 1 , wherein a microscope slide is installed at one side of the cover.5. The portable microscope device of claim 1 , wherein the cover is made by transparent material.6. The portable microscope device of claim 1 , wherein the base has a detective portion at the top thereof.7. The portable microscope device of claim 6 , wherein a power module is installed on the bottom of the base claim 6 , the illumination module corresponds to the ...

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Номер записи: 46
07-01-2016 дата публикации

Coherent fluorescence super-resolution microscopy

Номер: US20160004059A1
Автор: Amihai Meiri, Carl Ebeling, Jordan GERTON, Rajesh Menon, Zeev Zalevsky
Принадлежит: BAR ILAN UNIVERSITY, University of Utah Research Foundation UURF

A microscopy system which includes a light source for illuminating a sample; an objective lens for capturing light emitted from the illuminated sample to form a signal beam; and a dispersive optical element through which the signal beam is directed, wherein the dispersive optical element converts the signal beam to a spatially coherent signal beam.

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Номер записи: 47
07-01-2016 дата публикации

MICROSCOPY SLIDE SCANNER WITH VARIABLE MAGNIFICATION

Номер: US20160004062A1
Автор: Dixon Arthur Edward
Принадлежит:

An instrument and a method of scanning a large microscope specimen moves the specimen relative to a detector array during scanning by a scanner. Magnification of the instrument is adjustable using a zoom tube lens over a continuous range of magnification to enable scans of the specimen to be taken over a range of resolutions without varying the infinity corrective objective. Scans of the specimen can be taken over a range of resolutions with the same infinity connected objective. 1. An instrument for scanning a large microscope specimen , the instrument comprising a detector array that is part of an optical train to focus light from the specimen onto the detector array , the specimen being movable relative to the detector array , the optical train having an infinity corrected objective , the specimen being mounted on a support and moving relative to the detector array during scanning by a scanner , the instrument having a magnification that is adjustable using a zoom tube lens over a continuous range of magnification to enable scans of the specimen to be taken over a range of resolutions with the same infinity corrected objective.2. (canceled)3. (canceled)4. (canceled)5. (canceled)6. (canceled)7. (canceled)8. (canceled)9. (canceled)10. (canceled)11. (canceled)12. (canceled)13. (canceled)14. (canceled)15. (canceled)16. (canceled)17. (canceled)18. (canceled)19. (canceled)20. (canceled)21. (canceled)22. (canceled)23. (canceled)24. (canceled)25. (canceled)26. A method for scanning a large microscope specimen using an instrument having a detector array that is part of an optical train to focus light from the specimen onto the detector array , the optical train having an infinity corrected objective , the method comprising moving the specimen relative to the detector array during scanning by a scanner , adjusting the magnification over a range using a zoom tube lens to enable scans of the specimen to be taken over a range of resolutions with the same infinity corrected ...

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Номер записи: 48
07-01-2016 дата публикации

MICROSCOPY IMAGING DEVICE WITH ADVANCED IMAGING PROPERTIES

Номер: US20160004063A1
Автор: Burns Laurie, Cocker Eric, El Gamal Abbas, Ghosh Kunal, Ho Tatt Wei, Schnitzer Mark J.
Принадлежит:

Systems, methods and devices are implemented for microscope imaging solutions. One embodiment of the present disclosure is directed toward an epifluorescence microscope. The microscope includes an image capture circuit including an array of optical sensor. An optical arrangement is configured to direct excitation light of less than about 1 mW to a target object in a field of view of that is at least 0.5 mmand to direct epi-fluorescence emission caused by the excitation light to the array of optical sensors. The optical arrangement and array of optical sensors are each sufficiently close to the target object to provide at least 2.5 μm resolution for an image of the field of view. 1. An epifluorescence microscopy system , the system comprising:an optical excitation arrangement configured to direct light over an area encompassed within a field of view containing an imaging target along a specimen plane;an imaging circuit including an optical sensor array configured to generate image data from fluorescence caused by an interaction between the directed light and the imaging target;a synchronization circuit in communication with the imaging circuit and configured to interface with an external optical-data processing system that provides visual feedback of the image data; andan optical arrangement configured to direct the fluorescence through a dichroic mirror to the optical sensor array with sufficient intensity and focus for the image data to reach cellular level brain imaging,wherein the epifluorescence microscopy system is configured to be attached and reattached to a base plate of a supportive structure for allowing precise alignment of the epifluorescence microscopy system for repeated imaging of the field of view containing the imaging target during chronic experiments, andwherein the epifluorescence microscopy system has a dimension parallel to the specimen plane that does not exceed 1 inch.2. The epifluorescence microscopy system of claim 1 , wherein the optical ...

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Номер записи: 49
02-01-2020 дата публикации

Method and Device for Superresolution Optical Measurement using Singular Optics

Номер: US20200003551A1
Автор: Sirat Gabriel Y.
Принадлежит:

An optical method of measurement and an optical apparatus for determining the spatial position of at least one luminous object on a sample. A sequence of at least two compact luminous distributions of different topological families is projected onto the sample, and light re-emitted by the luminous object is detected. At least one optical image is generated for each luminous distribution on the basis of the light detected. The optical images are analyzed to obtain spatiotemporal information regarding the light re-emitted by the luminous object, or location of the luminous object. 1projecting onto the sample a first set of sequences, the first set having at least two members, of first and second compact luminous distributions, wherein the first and second compact luminous distributions are of distinct topological families and the sequences in the first set differ from one another by an attribute selected from the group consisting of projection axis, wavelength, and combinations thereof;projecting a second set of sequences, the second set having at least two members, of compact light distributions relative to a hypothesized position of the at least one luminous object, each compact light distribution characterized by a center, in such a manner that there is substantially zero intensity at the center of each compact light distribution and the sequences in the second set differ from one another by an attribute separately selected from the group consisting of projection axis, wavelength, and combinations thereof;detecting light re-emitted by the at least one object in the sample; andobtaining spatiotemporal information with respect to the light reemitted by the at least one luminous object on a basis selected from the group consisting of directly detecting a plurality of images, algorithmically analyzing the plurality of images, and combinations thereof.. A method of optical measurement for determining a spatial position of at least one luminous object in a sample, the ...

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Номер записи: 50
04-01-2018 дата публикации

High content imaging system and a method of operating the high content imaging system

Номер: US20180003941A1
Автор: Loren A. STAUFFER
Принадлежит: Molecular Devices LLC

A high content imaging system and a method of operating the high content imaging system are disclosed. A microscope has a first objective lens and a second objective lens, and an objective lens database has first and second transformation values associated with the first and the second objective lenses, respectively. A microscope controller operates the microscope with the first objective lens to develop first values of acquisition parameters. A configuration module automatically determines second values of the acquisition parameters using the first values of the acquisition parameters, first transformation values associated with the first objective lens, and second transformation values associated with the second objective lens. The microscope controller operates the microscope using the second objective lens and the second values of the acquisition parameters.

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Номер записи: 51
02-01-2020 дата публикации

LIGHT SHEET MICROSCOPE AND MICROSCOPIC METHOD USING A LIGHT SHEET MICROSCOPE

Номер: US20200004002A1
Автор: Fahrbach Florian, Tille Sebastian
Принадлежит:

A light sheet microscope includes a specimen-side objective having an illumination device configured to provide a first illumination beam which is focused for forming a first light sheet for illuminating a specimen from a first direction, the first light sheet being inclined obliquely in relation to an optical axis of the objective and being guided through the objective. A detector is configured to detect light passing through the objective. The illumination device is further configured to provide at least one second illumination beam which is focused for forming at least one second light sheet for illuminating the specimen from at least one second direction that differs from the first direction, the at least one second light sheet being inclined obliquely in relation to the optical axis of the objective and being guided through the objective. 1. A light sheet microscope comprising:a specimen-side objective having an illumination device configured to provide a first illumination beam, which is focused for forming a first light sheet for illuminating a specimen from a first direction, the first light sheet being inclined obliquely in relation to an optical axis of the objective and being guided through the objective; anda detector configured to detect light passing through the objective, andwherein the illumination device is further configured to provide at least one second illumination beam, which is focused for forming at least one second light sheet for illuminating the specimen from at least one second direction that differs from the first direction, the at least one second light sheet being inclined obliquely in relation to the optical axis of the objective and being guided through the objective.2. The light sheet microscope according to claim 1 , wherein the first direction of the first illumination beam and the at least one second direction of the at least one second illumination beam are perpendicular to each other or approximately perpendicular to each other ...

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Номер записи: 52
02-01-2020 дата публикации

Illumination Apparatus Optimized for Synthetic Aperture Optics Imaging Using Minimum Selective Excitation Patterns

Номер: US20200004040A1
Автор: Lee Chun-Sheu, Park Jong Buhm
Принадлежит:

A synthetic aperture optics (SAO) imaging method minimizes the number of selective excitation patterns used to illuminate the imaging target, based on the objects' physical characteristics corresponding to spatial frequency content from the illuminated target and/or one or more parameters of the optical imaging system used for SAO. With the minimized number of selective excitation patterns, the time required to perform SAO is reduced dramatically, thereby allowing SAO to be used with DNA sequencing applications that require massive parallelization for cost reduction and high throughput. In addition, an SAO apparatus optimized to perform the SAO method is provided. The SAO apparatus includes a plurality of interference pattern generation modules that can be arranged in a half-ring shape. 1. An apparatus for performing synthetic aperture optics (SAO) on a target including one or more objects , the apparatus comprising: a beam splitter for splitting a first laser beam into the pair of laser beams including a second laser beam and a third laser beam; and', 'a rotating window configured to modulate an optical path length of the third laser beam, wherein the second laser beam and the modulated third laser beam interfere to generate the selective excitation pattern on the target, and wherein the plurality of phases of the selective excitation pattern of the IPGM is generated by adjusting the optical path length of the third laser beam; and, 'a plurality of interference pattern generation modules (IPGMs), each IPGM configured to generate a pair of light beams that interfere to generate a selective excitation pattern illuminating the target at a predetermined orientation and a predetermined pitch, each IPGM further configured to generate a plurality of phases of the selective excitation pattern, each IPGM further includingan optical imaging module configured to optically image the illuminated target at a first resolution, the optical imaging module further configured to ...

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Номер записи: 53
03-01-2019 дата публикации

SPECTRAL MICROSCOPE

Номер: US20190003892A1
Автор: Aieta Francesco, Rogacs Anita, Santori Charles M.
Принадлежит: Hewlett-Packard Development Company ,L.P.

In one implementation, a spectral microscope may comprise a substrate with a planar lens, the planar lens including a phase profile including an axial focus and an oblique focus, a light source to excite a signal of a particle among a plurality of particles, and a detector to receive light generated from the light source from the axial focus of the planar lens and a spectral color component of the excited signal of the particle from the oblique focus of the planar lens. 1. A spectral microscope , comprising:a substrate with a planar lens, the planar lens including a phase profile including an axial focus and an oblique focus;a light source to excite a signal of a particle among a plurality of particles; and light generated from the light source from the axial focus of the planar lens; and', 'a spectral color component of the excited signal of the particle from the oblique focus of the planar lens., 'a detector to receive2. The spectral microscope of claim 1 , wherein the plurality of particles are located in a fluid.3. The spectral microscope of claim 1 , wherein the spectral color component of the oblique focus corresponds to a wavelength of the excited signal.4. The spectral microscope of claim 1 , wherein the phase profile of the planar lens includes:an axial phase profile based on the axial focus of the planar lens; andan oblique phase profile based on the oblique focus of the planar lens.5. The spectral microscope of claim 1 , wherein the planar lens is a diffractive lens.6. The spectral microscope of claim 1 , wherein the light source is a light emitting diode.7. The spectral microscope of claim 1 , wherein the light source is a laser.8. A method claim 1 , comprising:generating, by a light source, an excitation light to excite a plurality of fluorescent signals of a plurality of particles of a fluid, wherein the fluid is located in a channel in a transparent chip;receiving, at a detector from an axial focus of a planar lens, the excitation light;receiving, at ...

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Номер записи: 54
03-01-2019 дата публикации

Method for creating a digital fluorescent image

Номер: US20190003967A1
Автор: Ralf Wolleschensky, Thomas Kalkbrenner
Принадлежит: CARL ZEISS MICROSCOPY GMBH

In a method for creating a digital fluorescent image, the light emitted per pixel from an object plane is converted into a sequence of amplitudes, each of which is associated with one specific measurement time, the sequence of amplitudes is auto-correlated in a manner that is delayed by at least one time offset, and a specific correlation amplitude, from which a total amplitude is determined, is formed for each of the time offsets.

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Номер записи: 55
03-01-2019 дата публикации

Interferometric scattering microscopy

Номер: US20190004299A1
Автор: Alexander Weigel, Philipp Kukura
Принадлежит: Oxford University Innovation Ltd

An interferometric scattering microscope is adapted by performing spatial filtering of output light, which comprises both light scattered from a sample location and illuminating light reflected from the sample location, prior to detection of the output light. The spatial filtering passes the reflected illumination light but with a reduction in intensity that is greater within a predetermined numerical aperture than at larger numerical apertures. This enhances the imaging contrast for coherent illumination, particularly for objects that are weak scatterers.

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Номер записи: 56
03-01-2019 дата публикации

ILLUMINATION SYSTEM, MICROSCOPE COMPRISING AN ILLUMINATION SYSTEM AND MICROSCOPE METHOD

Номер: US20190004303A1
Автор: Themelis George
Принадлежит:

The invention relates to an illumination system () for a fluorescence microscope () for observation of an object () containing at least one fluorophore (), to a microscope () and to a microscope method for illumination of an object () comprising at least one fluorophore (). Solutions of the art have the disadvantage that orientation within an object () is difficult and visibility of fluorescing regions of the object () is non satisfying. The inventive illumination system () improves the visibility of an object () under study by comprising an illumination device (), with an emission spectrum () which includes fluorescent excitation wavelengths () of the at least one fluorophore () and visible-light background wavelengths (), further comprising a illumination filter () having at least one fluorescence excitation passband () and at least one background illumination passband (), wherein the transmissivity ()/width () of the fluorescence excitation passband () is larger/smaller than the transmissivity ()/width () of the background illumination passband (). The inventive illumination system () is adapted to perform the inventive microscope method. 11031719. An illumination system () for a fluorescence microscope () for observation of an object () containing at least one fluorophore () , comprising:{'b': 9', '9', '22', '23', '19', '25', '23, 'an illumination device (), the illumination device () having an emission spectrum () which comprises fluorescence excitation wavelengths () of the at least one fluorophore () and visible-light background illumination wavelengths () which differ from the fluorescence excitation wavelengths (),'}{'b': 41', '9', '17', '41', '93', '23', '95', '25, 'an illumination filter () for arrangement between the illumination device () and the object (), the illumination filter () having at least one fluorescence excitation passband () which is limited to the fluorescence excitation wavelengths () and at least one background illumination passband () ...

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Номер записи: 57
03-01-2019 дата публикации

DEVICE AND METHOD FOR CAPTURING IMAGES

Номер: US20190004304A1
Автор: GAIDUK Alexander, Sticker Markus, Stoppe Lars, Wolleschensky Ralf
Принадлежит:

A device for recording images is provided, an image-recording device and an illumination device being arranged on the same side of a specimen plane in said device. The image-recording device has illumination portions, for example individual light sources, which are actuatable independently of one another in order to be able to illuminate a specimen in the specimen plane at different angles and/or from different directions. In this way, it is possible to record a plurality of images with different illuminations, which can be combined to form a results image with improved properties. 1. A device for recording images , comprising:an image-recording device; andan illumination device arranged around an optical axis of the image-recording device,wherein the illumination device has a cut-out to facilitate a passage of light to the image-recording device, andwherein the illumination device has a plurality of independently actuatable illumination portions,wherein the image-recording device and the illumination device are arranged on the same side of a specimen plane of the image-recording device, andwherein an angle between adjacent illumination portions of the illumination device as seen from the specimen plane is less than or equal to an angle defined by a numerical aperture of the image-recording device.2. The device of claim 1 , wherein the angle between adjacent illumination portions of the illumination device is at least 10% smaller than the angle defined by the numerical aperture of the image-recording device.3. The device of claim 1 , wherein the plurality of illumination portions comprise a plurality of light source elements claim 1 ,wherein the light source elements are actuatable individually and/or segment-by-segment.4. (canceled)5. The device of claim 3 , wherein different light source elements have different properties in respect of the spectral range and/or polarization.6. The device of claim 1 , wherein the illumination device comprises an areal light source ...

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Номер записи: 58
11-01-2018 дата публикации

METHODS AND SYSTEMS FOR GENERATING NON-DIFFRACTING LIGHT SHEETS FOR MULTICOLOR FLUORESCENCE MICROSCOPY

Номер: US20180011303A1
Автор: DU Shengwang, LAU Sze Cheung, LOY Ming Tak Michael, SU Yumian, Wang Ying, ZHAO Teng
Принадлежит:

Methods and systems for generating non-diffracting light sheets for multicolor fluorescence microscopy are disclosed. A method for generating a non-diffracting light patterned Bessel sheet comprises transmitting an input light beam through a Fourier transform lens the input light beam has a spatial intensity pattern at a first plane, and a Fourier plane is formed after the Fourier transform lens to obtain a first light beam; transmitting the first light beam through an annulus mask to obtain a second light beam; and transmitting the second light beam through an excitation objective lens to form a non-diffracting patterned light sheet. A method for generating a non-diffracting light line Bessel sheet comprises transmitting an input light beam at a first lane through an annulus mask to obtain a first light beam; and transmitting the first light beam through an excitation objective lens to form a non-diffracting Bessel light sheet. 1. A method for generating a non-diffracting light patterned Bessel sheet (PBS) , comprising:transmitting an input light beam through a Fourier transform lens to obtain a first light beam, wherein the input light beam has a spatial intensity pattern at a first plane;transmitting the first light beam through an annulus mask arranged at a Fourier plane formed after the Fourier transform lens to obtain a second light beam; andtransmitting the second light beam through an excitation objective lens to form a non-diffracting patterned light sheet.2. A method for generating a non-diffracting light line Bessel sheet (LBS) , comprising:transmitting an input light beam that has a narrower intensity distribution along a first direction than that along a second direction vertical to the first direction at a first plane through an annulus mask arranged at the first plane to obtain a first light beam; andtransmitting the first light beam through an excitation objective lens to form a non-diffracting Bessel light sheet.3. A system for generating a non- ...

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Номер записи: 59
10-01-2019 дата публикации

TOTAL INTERNAL REFLECTION FLUORESCENCE IMAGING SYSTEM AND SEQUENCING DEVICE

Номер: US20190011365A1
Автор: Ge Liangjin, Wu Ping, Yan Qin, Zhao Luyang
Принадлежит: DIRECT GENOMICS CO., LTD.

The present disclosure discloses a total internal reflection fluorescence imaging system and a sequencing device. The total internal reflection fluorescence imaging system includes a first imaging system and a second imaging system. The first imaging system includes N separate laser light paths. The second imaging system determines the changes of the height of the sample by means of the differences of the positions at which the second receiving device acquires the second images, and performs automatic compensation according to the changes of the height of the sample, so as to ensure that the sample is always at a focused position. The embodiment can perform light path adjustment of the N separate laser light paths respectively by means of the second imaging system, so as to realize that the N separate laser light paths have the same penetration depth. Therefore no further light path adjustment is required when switching between different light sources, thus saving the time consuming for light path adjustment. 1. A total internal reflection fluorescence imaging system , comprising:a first imaging system, wherein the first imaging system comprises N separate laser light paths, an objective lens, and a first receiving device, N is an integer greater than one, each of the laser light path is configured to generate light to enter the objective lens, the light passing through the objective lens illuminates a sample to excite the sample to emit fluorescence, the fluorescence passes through the objective lens and enters the first receiving device to form a first image; anda second imaging system, wherein the second imaging system comprises a light emitting source, the objective lens, and a second receiving device, the light emitting source is configured to emit light to enter the objective lens, the light passing through the objective lens illuminates the sample and is reflected, the reflected light passes through the objective lens and enters the second receiving device to ...

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Номер записи: 60
14-01-2021 дата публикации

LIGHT DISC MICROSCOPY FOR FLUORESCENCE MICROSCOPES

Номер: US20210011269A1
Автор: Fadero Tanner Christian, Maddox Paul Samuel
Принадлежит:

Methods for imaging a sample using fluorescence microscopy, systems for imaging a sample using fluorescence microscopy, and illumination systems for fluorescence microscopes. In some examples, a method includes positioning the sample such that a plane of interest of the sample is coplanar with a focal plane of a detection objective of a microscope. The method includes positioning a paraboloidal minor around the sample such that a focal point of the paraboloidal mirror is coplanar with the focal plane of the detection objective and the plane of interest of the sample. The method includes directing a beam of annularly collimated excitation light on the paraboloidal minor to focus a disc of light on the sample and thereby to provide 360 degree lateral illumination of the sample. The method includes imaging the sample through the detection objective. 1. A method for imaging a sample using fluorescence microscopy , the method comprising:positioning the sample such that a plane of interest of the sample is coplanar with a focal plane of a detection objective of a microscope;positioning a paraboloidal mirror around the sample such that a focal point of the paraboloidal mirror is coplanar with the focal plane of the detection objective and the plane of interest of the sample;directing a beam of annularly collimated excitation light on the paraboloidal mirror to focus a disc of light on the sample and thereby to provide 360 degree lateral illumination of the sample; andimaging the sample through the detection objective.2. The method of claim 1 , wherein directing the beam of annularly collimated excitation light comprises setting a thickness of the beam of annularly collimated excitation light such that a propagation axis of each coplanar light sheet reflected by the paraboloidal mirror is at an oblique angle relative to the focal plane of the detection objective.3. The method of claim 2 , wherein setting the thickness of the beam of annularly collimated excitation light ...

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Номер записи: 61
09-01-2020 дата публикации

SUPER-RESOLUTION MICROSCOPY

Номер: US20200012121A1
Автор: Martin-Fernandez Marisa, WANG Lin
Принадлежит:

We describe a super-resolution optical microscopy technique in which a sample is located on or adjacent to the planar surface of an aplanatic solid immersion lens and placed in a cryogenic environment. 1. A method of carrying out super-resolution optical microscopy on a sample , comprising:providing an aplanatic solid immersion lens having a planar surface;locating the sample on or adjacent to the planar surface of the aplanatic solid immersion lens;locating the sample in a cryogenic environment; andimaging the sample through the aplanatic solid immersion lens using a super-resolution optical microscopy technique to provide a super-resolution image of the sample.2. The method of wherein the cryogenic environment is at least one of: at a temperature of less than 200 Kelvin claim 1 , at a temperature of less than 100 Kelvin; maintained using liquid nitrogen; and maintained using nitrogen vapour.3. The method of wherein the sample is in contact with the aplanatic solid immersion lens.4. The method of wherein the super-resolution optical microscopy technique is a single molecule localisation optical microscopy technique.5. The method of wherein the single molecule localisation optical microscopy technique comprises:labelling the sample with optical emitters;separately detecting each of a plurality of optical emissions, each optical emission being from a single one of the emitters, the optical emissions being received at a detector through the aplanatic solid immersion lens while the sample is located in the cryogenic environment; andconstructing the super-resolution image of the sample from the separately detected optical emissions.6. The method of wherein the optical emitters comprise fluorophores such as organic dyes claim 5 , fluorescent proteins and quantum dots.7. The method of wherein each fluorophore is a fluorophore molecule for bonding to a molecule of the sample.8. The method of wherein the single molecule localisation optical microscopy technique is one or ...

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Номер записи: 62
03-02-2022 дата публикации

Optical arrangment for fluorescence microscopy applications

Номер: US20220034813A1
Автор: Falk Eilenberger, Frank Setzpfand, Markus GRAEFE, Marta Gilaberte Basset
Принадлежит: Fraunhofer Gesellschaft zur Forderung der Angewandten Forschung eV, Friedrich Schiller Universtaet Jena FSU

In the optical arrangement for fluorescent microscopic applications, one or more multiphoton beams, but at least one or two photon pair beams, from a source of non-classical light is/are directed at a first optical system, consisting of an arrangement of at least one lens or one photon-reflecting element or another beam-forming element or a combination thereof. The first optical system (3) is designed to shape the non-classical light into a light sheet (4) or a light sheet-like shape and thence to direct it at a specimen (5), so that fluorescent radiation is excited by means of multiphoton absorption using the multiple multiphoton beams that are simultaneously incident on/in the specimen. Fluorescent radiation (6) obtained by excitation is incident by means of a second optical system (7) on a detection system (8) that is designed for the spatially resolved capture of fluorescent radiation.

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Номер записи: 63
03-02-2022 дата публикации

MICRODEVICE AND ANALYSIS DEVICE

Номер: US20220034876A1
Автор: MIZOKUCHI Chikaaki, SHIGEMURA Koji, SUMIYOSHI Ken
Принадлежит: TIANMA JAPAN, LTD.

A microdevice includes: a microchannel to which a measurement target solution containing a measurement target substance is introduced; an antibody being fixed to at least one sidewall surface of the microchannel and specifically binding to the measurement target substance; a fluorescence-labeled derivative being specifically bound to the antibody and being acquired by fluorescence-labeling the measurement target substance; and a light blocker blocking excitation light exciting fluorescent light radiated by the fluorescence-labeled derivative. The measurement target substance and the fluorescence-labeled derivative specifically bind to the antibody in a competitive manner, and the antibody is fixed to the sidewall surface of the microchannel in a state of specifically binding to the fluorescence-labeled derivative. The light blocker blocks the excitation light entering the fluorescence-labeled derivative specifically binding to the antibody. 1. A microdevice comprising:a microchannel to which a measurement target solution containing a measurement target substance is introduced;an antibody being fixed to at least one sidewall surface of the microchannel and specifically binding to the measurement target substance;a fluorescence-labeled derivative being specifically bound to the antibody and being acquired by fluorescence-labeling the measurement target substance; anda light blocker blocking excitation light exciting fluorescent light radiated by the fluorescence-labeled derivative, whereinthe measurement target substance and the fluorescence-labeled derivative specifically bind to the antibody in a competitive manner,the antibody is fixed to the sidewall surface of the microchannel in a state of specifically binding to the fluorescence-labeled derivative, andthe light blocker blocks the excitation light entering the fluorescence-labeled derivative specifically binding to the antibody.2. The microdevice according to claim 1 , whereinthe antibody is a plurality of the ...

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Номер записи: 64
03-02-2022 дата публикации

FILTER MODULE AND PROJECTION APPARATUS

Номер: US20220035081A1
Автор: Chien Cheng-Che, Lin Sheng-Chiang, Weng Ming-Tsung
Принадлежит: CORETRONIC CORPORATION

A filter module and a projection apparatus are provided. The filter module includes a filter layer and a diffusion layer. The filter layer includes a first filter region and a second filter region, which respectively allow light having a first waveband and light having a second waveband to pass through. The diffusion layer is disposed on a side of the filter module opposite to the filter layer and includes a first diffusion portion with a first haze value and a second diffusion portion with a second haze value. The first diffusion portion is disposed corresponding to the first filter region and allows the light having the first waveband to pass through. The second diffusion portion is disposed corresponding to the second filter region and allows the light having the second waveband to pass through. The first haze value is different from the second haze value. 1. A filter module , comprising a filter layer and a diffusion layer , wherein:the filter layer comprises a first filter region and a second filter region, the first filter region and the second filter region respectively allow light having a first waveband and light having a second waveband to pass through; andthe diffusion layer is disposed on a side of the filter module opposite to the filter layer and comprises a first diffusion portion with a first haze value and a second diffusion portion with a second haze value, the first diffusion portion is disposed corresponding to the first filter region and allows the corresponding light having the first waveband to pass through, and the second diffusion portion is arranged corresponding to the second filter region and allows the corresponding light having the second waveband to pass through, and the first haze value is different from the second haze value.2. The filter module as claimed in claim 1 , wherein a peak value of the first waveband is greater than a peak value of the second waveband claim 1 , and the first haze value is less than the second haze value.3. ...

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Номер записи: 65
03-02-2022 дата публикации

MICROSCOPIC TRANSMITTED LIGHT CONTRASTING METHOD

Номер: US20220035146A1
Автор: Schumann Christian
Принадлежит:

A microscopic transmitted light contrasting method includes illuminating a sample through asymmetrical first and second illumination pupils and imaging the sample through asymmetrical first and second detection pupil in order to generate, respectively, first and second partial images. The first illumination pupil and the first detection pupil, as well as the second illumination pupil and the second detection pupil, are arranged pivoted in relation to one another and partially overlapping in projection on a plane perpendicular to an optical axis in such a way that first and third regions of an angular space are in a bright field and second and fourth regions of the angular space are in a dark field, and the first and second partial images each have a bright and a dark field component. An image of the sample is generated from the first and second partial images. 1. A microscopic transmitted light contrasting method , the method comprising:at least partially illuminating a sample through an asymmetrical first illumination pupil;at least partially imaging the sample through an asymmetrical first detection pupil in order to generate a first partial image, wherein the first illumination pupil and the first detection pupil are arranged pivoted in relation to one another and partially overlapping one another in projection on a plane perpendicular to an optical axis in such a way that at least one first region of an angular space is in a bright field and one second region of the angular space different from the first region is in a dark field, and the first partial image has a first bright field component and a first dark field component;at least partially illuminating the sample through an asymmetrical second illumination pupil;at least partially imaging the sample through an asymmetrical second detection pupil in order to generate a second partial image, wherein the second illumination pupil and the second detection pupil are arranged pivoted in relation to one another and ...

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Номер записи: 66
03-02-2022 дата публикации

IMAGING SYSTEMS WITH MICRO OPTICAL ELEMENT ARRAYS AND METHODS OF SPECIMEN IMAGING

Номер: US20220035147A1
Автор: Horisberger Aurèle Timothée, Joss Diego, Naumenko Andrey, Pirolet Jonathan Abel, Rachet Bastien, Schmitt Frédéric, Shaffer Etienne
Принадлежит:

Disclosed herein are systems for imaging of samples using an array of micro optical elements and methods of their use. In some embodiments, an optical chip comprising an array of micro optical elements moves relative to an imaging window and a detector in order to scan over a sample to produce an image. A focal plane can reside within a sample or on its surface during imaging. Detecting optics are used to detect back-emitted light collected by an array of micro optical elements that is generated by an illumination beam impinging on a sample. In some embodiments, an imaging system has a large field of view and a large optical chip such that an entire surface of a sample can be imaged quickly. In some embodiments, a sample is accessible by a user during imaging due to the sample being exposed while disposed on or over an imaging window. 1. A system for imaging of a sample , the system comprising:a transparent imaging window, wherein the transparent imaging window provides a surface onto or over which a sample is disposed during imaging of the sample;a photon source for providing an illumination beam;a beam splitter for directing the illumination beam toward the imaging window;a collimating lens for collimating the illumination beam over an area comparable in size to a field to be illuminated, thereby providing a collimated illumination beam, wherein the collimating lens is disposed between the imaging window and the beam splitter such that the illumination beam is directed to the collimating lens by the beam splitter; (i) focuses a portion of the collimated illumination beam onto a tight focus, and', '(ii) directs back-emitted light from the sample through the collimating lens and towards the beam splitter;, 'an optical chip, wherein the optical chip comprises an array of micro optical elements for focusing the collimated illumination beam onto a focal plane above or on the imaging window, wherein each micro optical element of the array of micro optical elementsa ...

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Номер записи: 67
19-01-2017 дата публикации

BIOMOLECULE ANALYSIS DEVICE

Номер: US20170016814A1
Автор: Kajiyama Tomoharu, KAMBARA Hideki, SHIRAI Masataka
Принадлежит: Hitachi, Ltd.

An object is to provide a biomolecule analyzer capable of collecting and analyzing a biomolecule in a single cell without damaging neighboring cells. 1. A biomolecule analyzer , comprising:a first unit configured to obtain an optical image of a plurality of cells;a second unit configured to disrupt a part or the whole of at least one cell of the plurality of cells;an array device in which regions for capturing a biomolecule in the cell released by the disrupting unit are arranged; anda third unit configured to associate the region in which the biomolecule is captured in the array device with a portion corresponding to the cell disrupted by the disrupting unit in the optical image.2. The biomolecule analyzer according to claim 1 , further comprising a fourth unit configured to place the region of the array device and the cell to be disrupted in close proximity of each other before the cell is disrupted by the disrupting unit.3. The biomolecule analyzer according to claim 1 , further comprising a fifth unit configured to suck the biomolecule in the cell to be released into the region of the array device or allows the biomolecule to migrate into the region.4. The biomolecule analyzer according to claim 1 , wherein the array device is a porous membrane or a bead array having beads packed on the surface thereof.5. The biomolecule analyzer according to claim 1 , wherein a probe molecule for selectively capturing the biomolecule in the cell is immobilized on at least one of the surface and in the inside of the array device.6. The biomolecule analyzer according to claim 5 , wherein the biomolecule in the cell is mRNA claim 5 , and the probe molecule is a DNA probe.7. The biomolecule analyzer according to claim 6 , wherein the DNA probe has a sequence which is different for each position of the array device.8. The biomolecule analyzer according to claim 5 , wherein the biomolecule in the cell is a protein or a peptide claim 5 , and the probe molecule is an antibody.9. The ...

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Номер записи: 68
19-01-2017 дата публикации

ENHANCEMENT OF VIDEO-RATE FLUORESCENCE IMAGERY COLLECTED IN THE SECOND NEAR-INFRARED OPTICAL WINDOW

Номер: US20170017069A1
Автор: Bardhan Neelkanth M., Belcher Angela M., Rajan Nandini, Siegel Andrew M.
Принадлежит:

System and method configured to operate under conditions when the object being imaged destroys or negates the information which otherwise allows the user to take advantage of optical parallax, configured to elicit luminescence from the same targets in the object as a result of irradiation of these targets with pump light at different, respectively corresponding wavelengths, and acquire optical data from so-illuminated targets through the very same optical path to image the object at different wavelengths. One embodiment enables acquisition, by the same optical detector and from the same object, of imaging data that includes a reflectance image and multiple fluorescence-based images caused by light at different wavelengths, to assess difference in depths of locations of targets within the object. 1. An imaging system comprising:an optical system including an optical train of components and a single optical detector in optical communication with said optical train, the single optical detector having a spectral band of operation, andan illumination system configured to emit first light and second light at respectively-corresponding first and second wavelengths,wherein the first light is received in direct reflection of light, that has been emitted by the illumination system, from the object andwherein the first fluorescent light includes fluorescence generated, at a first fluorescence wavelength, at a first portion of the object as a result of interaction of the object with the second light,wherein both the first wavelength and the first fluorescence wavelengths are within the spectral band of operation, andwherein the first and second wavelengths are defined such that, during imaging of a chosen object, the first light and first fluorescent light are received by the single optical detector along the same optical path through said optical train.2. An imaging system according to claim 1 ,wherein the spectral band of operation is defined within a second near-infrared ( ...

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Номер записи: 69
19-01-2017 дата публикации

OPTICAL SYSTEM COMPRISING A SPATIAL LIGHT MODULATOR

Номер: US20170017076A1
Автор: Emiliani Valentina, Guillon Marc, Papagiakoumou Eirini
Принадлежит:

The invention concerns an optical system () comprising: 2301718. The optical system according to claim 1 , wherein for a same power of the unmodulated “zero order” part in the replay volume claim 1 , the perturbing aberrant optical element () decreases by a factor ten or more claim 1 , a maximum intensity of the unmodulated “zero order” part of the modulated light beam () in the replay volume ().3301217. The optical system according to one of to claim 1 , wherein the perturbing aberrant optical element () is configured for spatially modulating a phase of the incident light beam () and/or the modulated light beam ().430. The optical system according to claim 3 , wherein the aberrations introduced by the perturbing aberrant optical element () have spatial frequencies that are less than or equal to 1/(2a) claim 3 , where a is a period of the array of controllable elements.513. The optical system according to one of to claim 3 , wherein the spatial light modulator () is a spatial phase modulator.6301217. The optical system according to one of to claim 3 , wherein the perturbing aberrant optical element () comprises a lens having a cylindrical surface claim 3 , for spreading the incident light beam () and/or the modulated light beam () according to a spreading direction.7301217. The optical system according to one of to claim 3 , wherein the perturbing aberrant optical element () comprises a first lens having a first cylindrical surface and a second lens having a second cylindrical surface claim 3 , for spreading the incident light beam () and/or the modulated light beam () according to a first spreading direction and to a second spreading direction defining an angle relative to the first spreading direction.8. The optical system according to one of to claim 3 , wherein:{'b': 18', '18, 'the modulated part carries the image to be projected into the replay volume () for exciting a light sensitive substance located in the replay volume (), and'}{'b': '17', 'the light ...

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Номер записи: 70
21-01-2021 дата публикации

METHODS AND APPARATUSES FOR SORTING TARGET PARTICLES

Номер: US20210016277A1
Автор: Dimov Ivan K., Fernhoff Nathaniel, Pradhan Lagnajeet
Принадлежит:

This disclosure provides methods and apparatuses for sorting target particles. In various embodiments, the disclosure provides a cassette for sorting target particles, methods for sorting target particles, methods of loading a microchannel for maintaining sample material viability, methods of quantifying sample material, and an optical apparatus for laser scanning and particle sorting. 1165.-. (canceled)166. A method of sorting cells , comprising screening cellular material to identify cells with a desired phenotype , wherein the cellular material is screened at a rate of 50 ,000 cells per second or greater.167. The method of claim 166 , wherein the cellular material is screened at a rate 500 claim 166 ,000 cells per second or greater.168. The method of claim 166 , wherein the cellular material is screened at a rate of 1 claim 166 ,000 claim 166 ,000 cells per second or greater.169. The method of claim 166 , wherein the method further comprises extracting said cells of a desired phenotype from said cellular material at a rate of 100 claim 166 ,000 cells per second or greater.170. The method of claim 169 , wherein said cells of a desired phenotype are extracted at a rate of 300 claim 169 ,000 cells per second or greater.171. The method of claim 166 , wherein said cellular material is screened in an array with through-holes.172. The method of claim 171 , wherein said cells of aid desired phenotype are extracted from said array.173. The method of claim 172 , wherein said extracting comprises releasing said cells of said desired phenotype using electromagnetic radiation.174. The method of claim 173 , wherein each through-hole of said array comprises from 0 to about 5 cells and at least 30% of the through-holes of the array comprise at least one cell.175. The method of claim 166 , wherein the cellular material is obtained from a human subject and/or comprises less than 5% of HSCs and HSPCs.176. The method of claim 169 , wherein greater than 95% of the extracted cells are ...

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Номер записи: 71
21-01-2016 дата публикации

HIGH VERSATILE COMBINABLE MICROSCOPE BASE AND MICROSCOPE HAVING THE SAME

Номер: US20160018631A1
Автор: YANG Chih-Yi
Принадлежит:

A combinable microscope base for mounting a light source and a microscopic image capturing device having an optical axis. The light source and the microscopic image capturing device are connected to a display processing device through signal transmission. The light source and the microscopic image capturing device have compatible first and second coupling portions. The combinable microscopic base includes a base body and a main support frame, the main support frame is formed with a placement unit and a main assembly port. The main assembly port is configured such that when the microscopic image capturing device or the light source is mounted therein, the optical axis of the microscopic image capturing device or a main light emitting direction of the light source is oriented toward and corresponds to the placement unit.

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Номер записи: 72
21-01-2016 дата публикации

RAPID CONFOCAL MICROSCOPY TO SUPPORT SURGICAL PROCEDURES

Номер: US20160018632A1
Автор: GAREAU Daniel S.
Принадлежит:

One embodiment of techniques for confocal microscopy includes illuminating a spot on a surface of a biological sample. A first emission intensity from the spot is detected in a first range of optical properties; and a second emission intensity in a second range. A pixel that corresponds to the spot is colored using a linear combination of the first and second emission intensities. Sometimes, the pixel is colored to approximate a color produced by histology. In some embodiments, a surface of a sample is contacted with a solution of a nucleus dye. Then, a spot is illuminated with a laser beam of wavelength about 488 nanometers (nm). Fluorescence emission intensity is detected above about 500 nm. Sometimes, a certain illumination correction is applied. In some embodiments, a sample holder that compresses a sample is removable from a stage that is fixed with respect to a focal plane of the microscope.

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Номер записи: 73
17-01-2019 дата публикации

FULLY AUTOMATED CONTINUOUS CELL CULTURE SYSTEM

Номер: US20190017009A1
Автор: Yu Xuejun
Принадлежит: Shanghai GenBase Biotechnology Co., Ltd.

The invention provides a full-automatic cell culture system, at least comprising: a control module, a control platform, a dark field microscope for on-line observation of cell culture, a cell incubator shaker and a cell culture bag. The control platform is connected to the dark field microscope for on-line observation of cell culture and the cell incubator shaker respectively, and the control module is connected to the control platform. The invention also provides a culture bag support frame for a continuous cell incubator shaker, the continuous cell incubator shaker, a non-contact sensor connector, the cell culture bag and the dark field microscope for on-line observation of cell culture which are related to the full-automatic cell culture system, thereby realizing continuous culture and observation of cells. 1. A full-automatic cell culture system , characterized by at least comprising: a control module , a control platform , a dark field microscope for on-line observation of cell culture , a cell incubator shaker , a cell culture bag and a power source module; whereinthe cell incubator shaker comprises a culture bag support frame, a swing frame and a shake device, the culture bag support frame is provided with an arc-shaped bottom plate and cell culture bag fixing parts, the swing frame comprises a swing base, the swing base is provided with support arms, the culture bag support frame and the swing frame are fixedly connected by the support arms, the shake device comprises a substrate, the substrate is provided with swing components, the swing base is connected to the swing components, and the arc-shaped bottom plate is provided with an object lens inserting gap;the cell culture bag comprises a closed bag body, and the middle of the bottom surface of the bag body is provided with a transparent observation area; and the bag body is disposed on the cell incubator shaker, and the transparent observation area is matched with the object lens inserting gap;the dark ...

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Номер записи: 74
19-01-2017 дата публикации

DEVICE FOR CORRELATIVE SCANNING TRANSMISSION ELECTRON MICROSCOPY (STEM) AND LIGHT MICROSCOPY

Номер: US20170018399A1
Автор: DE JONGE Niels
Принадлежит: LEIBNIZ-INSTITUT FUER NEUE MATERIALIEN GEMEINNUETZIGE GESELLSCHAFT MIT BESCHRAENKTER HAFT

The invention relates to a device tor correlative scanning transmission electron microscopy (STEM) and light microscopy. In order to create a device for correlative microscopy which enables an improved combination of light microscopy and STEM methods, a STEM detector () according to the invention is combined with a photo-optical lens (). This detection device combines the efficient detection by means of STEM microscopy of materials having a high atomic number, for example specific nanoparticle markers in a specimen in a liquid, such as a cell, with simultaneous light microscopy. 178. Device for correlative scanning transmission electron microscopy (STEM) and light microscopy , wherein a STEM detector () is combined with a photo-optical lens ().278. Device according to claim 1 , wherein the STEM detector () is integrated in a photo-optical lens ().3558. Device according to claim 2 , wherein the STEM detector () is positioned in a cavity () in the photo-optical lens ().4567. Device according to claim 3 , wherein the cavity () has claim 3 , at the specimen end claim 3 , a small-diameter opening followed by a conical electron drift chamber () at the lower end of which the STEM detector () is located.57. Device according to claim 1 , wherein the signal from the STEM detector () can be transmitted to the outside at the side of the lens.6281. Device according to claim 1 , wherein a specimen holder () is located at that end of the photo-optical lens () which is nearer to a specimen ()722. Device according to claim 6 , wherein the specimen holder () is configured as a thin claim 6 , electron-permeable membrane ().862. Device according to claim 6 , wherein the electron drift chamber () and the space surrounding the specimen holder () have the property of being able to establish a vacuum.927. Device according to claim 6 , wherein an electron beam source is located on the other side of the specimen holder () from the STEM detector ().108. Device according to claim 1 , wherein a ...

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Номер записи: 75
17-01-2019 дата публикации

SPECTRALLY-RESOLVED SCANNING MICROSCOPE

Номер: US20190018231A1
Автор: Dixon Arthur Edward
Принадлежит: Huron Technologies, International Inc.

A scanning microscope and method of operation has a scan filter with a repeat pattern of a plurality of rows that is repeated at least across an active area of an entire surface of an area detector. Each row is covered by a bandpass filter or an emission filter for a specific fluorophore or filters of a particular colour for each row. The scanning microscope can be used to obtain one or more of hyperspectral images, multispectral images, RGB images, RGBW images, W images and Single Field Of View images of a specimen using Moving Specimen Image Averaging. A method of obtaining one or more of the images is also described. 1. A scanning microscope for scanning and obtaining a hyperspectral image of at least a portion of a large microscope specimen , the microscope comprising:a) an illumination system to illuminate a part of the specimen being scanned;b) at least one lens that focuses light from the specimen onto an area detector, the specimen being mounted on a support that is movable relative to the area detector;c) the motion of the support being controlled by a computer, the motion of the support being in a direction perpendicular to rows of the area detector;d) the area detector having a scan filter with a repeat pattern of a plurality of rows that is repeated at least across an active area of an entire surface of the area detector, each row of the repeat pattern being covered by a bandpass filter, there being a plurality of repeat patterns and a plurality of bandpass filters having the same bandwidth, each bandpass filter transmitting a narrow spectral range, the bandpass filters forming a hyperspectral filter with a bandwidth that is equal to a sum of the bandwidths of the separate bandpass filters for each of the rows;e) the area detector is computer controlled to capture sequential substantially overlapping frame images of the specimen each time that an optical image of the specimen has moved a distance relative to the area detector that is equal to the ...

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Номер записи: 76
16-01-2020 дата публикации

Spectral imaging apparatus and methods

Номер: US20200018702A1
Автор: Kent A. Murphy, Philip R. Couch
Принадлежит: AXON DX LLC

An imaging system images a sample across one or more wavelengths. A light source illuminates a sample with one or more wave-lengths of light, and an image sensor detects light from the illuminated sample. A linear variable long pass filter is positioned to filter light reflected from the sample to pass to the image sensor multiple different wave-length bands having different cut-off wavelengths. Wavelengths of light on one side of the cut-off wavelength are blocked and wavelengths of light on the other side of the cut-off wavelength are passed as multiple different long pass wavelength bands for detection by the image sensor. The image sensor detects light for each of the multiple different long pass wavelength bands from the sample. Data processing circuitry converts the detected light for the multiple different long pass wavelength bands for the sample into corresponding different long pass wavelength band data sets for the sample.

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Номер записи: 77
21-01-2021 дата публикации

HIGH-THROUGHPUT HYPERSPECTRAL IMAGING SYSTEMS

Номер: US20210018440A1
Автор: Conrad Andrew, Santori Charles, Sinha Supriyo
Принадлежит: Verily Life Sciences LLC

High-throughput hyperspectral imaging systems are provided. According to an aspect of the invention, a system includes an excitation light source; an objective that is configured to image excitation light onto the sample, such that the excitation light causes the sample to emit fluorescence light; a channel separator that is configured to separate the fluorescence light into a plurality of spatially dispersed spectral channels; and a sensor. The excitation light source includes a light source and a plurality of lenslet arrays. Each of the lenslet arrays is configured to receive light from the light source and to generate a pattern of light, and the patterns of light generated by the lenslet arrays are combined to form the excitation light. The objective is configured to simultaneously image each of the patterns of light to form a plurality of parallel lines or an array of circular spots at different depths of the sample. 1. A system comprising:an excitation light source that is configured to emit excitation light;an objective that is configured to receive the excitation light from the excitation light source and image the excitation light onto a sample, such that the excitation light causes the sample to emit fluorescence light;a channel separator that is configured to receive the fluorescence light from the sample and separate the fluorescence light into a plurality of spatially dispersed spectral channels; anda sensor that is configured to receive the plurality of spatially dispersed spectral channels from the channel separator, a plurality of light sources, wherein each of the plurality of light sources emits light having a different wavelength;', 'a dichroic array that is configured to combine light from each of the plurality of light sources; and', 'a first lenslet array, wherein the first lenslet array is configured to receive light from the dichroic array and to generate a plurality of patterns of light corresponding to the plurality of light sources;, ' ...

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Номер записи: 78
21-01-2021 дата публикации

QUANTITATIVE LIQUID BIOPSY DIAGNOSTIC SYSTEM AND METHODS

Номер: US20210018441A1
Автор: Tafas Triantafyllos P., Winfree Seth
Принадлежит:

The present invention provides a quantitative liquid biopsy diagnostic system and methods for performing diagnostic assays. The system offers a liquid biopsy method using circulating tumor cells (CTCs) or White Blood Cells (WBC) subpopulations for precision cancer diagnosis, early detection of disease evolution, and cancer patient management. The invention utilizes selective plane illumination microscopy (SPIM) to deliver high sensitivity and specificity for the detection and isolation of individual CTCs, superseding the efficacy of existing methodologies for early cancer detection. Isolated CTCs can be analyzed for their molecular fingerprint, which can lead to matching genetic abnormalities with specific drug treatments. The system allows ex vivo observation of live CTC or WBC response to treatment. This observation of live cells offers the oncologist a new potential for optimizing therapeutic protocols by testing a patient's own cells, and then administering treatment to the patient with the expectation of improving efficacy and reducing toxicity to normal cells. 1. A method for characterizing and quantitating target cells in a blood sample comprising the steps of:(a) obtaining a blood sample from a subject, (i) centrifugation to separate cell layers from the blood serum layer', '(ii) removal of one of the cell layers,', '(iii) suspension of the removed layer from step (b) (ii) in a buffer,', '(iv) purification of the suspended layer of (b) (iii),', '(v) immobilization of the purified layer of (b) (iv), and', '(vi) immunostaining and/or fluorescence in situ hybridization (FISH) staining of the immobilized layer of (b) (v),, '(b) preparing the sample by one or more of the following steps (i) through (vi), comprising'}(c) subjecting the prepared sample from (b) to selective plane image microscopy by scanning the sample with a laser sheet light source at a multiple of cross sections to obtain contiguous cross-sectional images,(d) collecting a sufficient quantity of ...

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Номер записи: 79
16-01-2020 дата публикации

SYSTEM AND TECHNIQUES FOR DETECTING FLUORESCING PARTICLES ON A TARGET

Номер: US20200018942A1
Автор: Baldwin Tristan J., Brabant, Forgione Matteo, JR. Edward L., Mazel Charles H., Meade Michael J., Rochford Richard
Принадлежит:

The present disclosure relates to a system and technique for a system for detecting fluorescing particles on a target comprising a viewing device configured to view the target and a light subsystem configured to illuminate the target, wherein the target includes a fluorescing particle that effectuates the fluorescent feedback second wavelength. In one example, no additional fluorescing agent or additive, other than the fluorescing particle, is added to the target. A passive cooling subsystem is configured to prevent overheating of the system without expending additional energy resources. The system includes a filter in operative communication with the viewing device having a selected filter wavelength configured to allow light to pass that is greater than the first wavelength and at least one non-transitory computer readable storage medium having instructions encoded thereon. 1. A system for detecting fluorescing particles on a target , comprising:a viewing device configured to view the target;a light subsystem configured to illuminate the target and having a white light source and a blue light source integrated into a single unitary housing, wherein the blue light source emits light at a first wavelength towards the target adapted to excite particles on the target at a fluorescent feedback second wavelength generated in response to particle excitation from the blue light source, wherein the fluorescent feedback second wavelength is greater than the first wavelength;a passive cooling subsystem configured to prevent overheating of the system without expending additional energy resources; andat least one filter in operative communication with the viewing device having a selected filter wavelength configured to allow light to pass that is greater than the first wavelength.2. The system of claim 1 , wherein the light subsystem further comprises an ultraviolet light source in the single unitary housing.3. The system of claim 1 , wherein the passive cooling subsystem ...

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Номер записи: 80
16-01-2020 дата публикации

Optical arrangement and method for imaging a sample

Номер: US20200018943A1
Автор: Gustavo Quintas Glasner de Medeiros, Lars Hufnagel, Nils Norlin
Принадлежит: Europaisches Laboratorium fuer Molekularbiologie EMBL

An optical arrangement for imaging a sample is disclosed. The optical arrangement comprises at least one first objective lens and at least one second objective lens, at least one illumination source for producing an illumination beam, detector for imaging radiation from the sample, and at least one mirror for reflecting the radiation from one of the first objective lens or the second objective lens into the detector. The at least one mirror is double-sided and dependent on the illumination beam at the other one of the first objective lens and the second objective lens.

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Номер записи: 81
16-01-2020 дата публикации

ENDOSCOPE AND IMAGE PICKUP APPARATUS

Номер: US20200018947A1
Автор: TSUYUKI Hiroshi
Принадлежит: OLYMPUS CORPORATION

An endoscope includes in order from an object side to an image side, an objective optical system, an optical-path splitter which splits light from the objective optical system into two, and an image sensor which picks up two split images. 2. The endoscope according to claim 1 , wherein the multi-order λ/4 wavelength plate is adhered to an isotropic plate material.3. The endoscope according to claim 1 , wherein an anti-reflection coating is applied to a surface of the multi-order λ/4 wavelength plate.4. The endoscope according to claim 1 , wherein the birefringent material of the multi-order λ/4 wavelength plate is one of LiNbO claim 1 , YVO claim 1 , calcite claim 1 , and α-BBO.5. An image pickup apparatus claim 1 , comprising in order from an object side to an image side:an objective optical system;an optical-path splitter which splits light from the objective optical system into two; andan image sensor which picks up two split images, whereina multi-order λ/4 wavelength plate made of a birefringent material is disposed in an optical path between the objective optical system and the optical-path splitter, and [{'br': None, 'i': 'd/d', '10≤(0)×0.25≤85\u2003\u2003(1)'}, {'br': None, 'i': 'n|<', '0.08<|Δ0.24\u2003\u2003(2)′'}], 'the multi-order λ/4 wavelength plate satisfies the following conditional expressions (1) and (2)′'}where,d denotes a thickness of the multi-order λ/4 wavelength plate,d0 denotes a thickness of the multi-order λ/4 wavelength plate, which becomes zero-order for an e-line, andΔn denotes a birefringence for the e-line of the multi-order λ/4 wavelength plate.6. The image pickup apparatus according to claim 5 , wherein the multi-order λ/4 wavelength plate is adhered to an isotropic plate material.7. The image pickup apparatus according to claim 5 , wherein an anti-reflection coating is applied to a surface of the multi-order λ/4 wavelength plate.8. The image pickup apparatus according to claim 1 , wherein the birefringent material of the multi-order ...

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Номер записи: 82
21-01-2021 дата публикации

ILLUMINATION FILTER SYSTEM AND OBSERVATION SYSTEM FOR A MULTISPECTRAL FLUORESCENCE MICROSCOPE, MULTISPECTRAL FLUORESCENCE MICROSCOPE, AND MICROSCOPYING METHOD

Номер: US20210018738A1
Автор: Kuster Manfred, Themelis George
Принадлежит:

The present invention relates to an illumination filter system () for medical imaging, in particular multispectral fluorescence imaging, as performed e.g. in a microscope () or endoscope, such as a surgical microscope, in particular a surgical multispectral fluorescence microscope, comprising a first optical filter (). The present invention also relates to an observation system () for medical imaging, in particular multispectral fluorescence imaging, as performed e.g. in a microscope () or endoscope, in particular a multispectral fluorescence microscope, comprising a beam splitter () adapted to split a light image () into a first light portion () along a first light path () and a second light portion () along a second light path (). To improve known illumination filter systems and observation systems, so these systems work with one light source only, are capable of capturing simultaneously at least one fluorescence signal and a signal of visible reflected light and allow a homogeneous illumination for obtaining different images from the object illuminated, the first optical filter () is adapted to quench light of at least one fluorescence excitation band within the visible spectrum in the illumination filter system () of the present invention, and the first light portion () comprises at least one fluorescence emission band (Em., Em.) in the visible spectrum and the second light portion () portion comprises a visible reflected light (VISR) in the observation system () of the present invention. 1. An observation system for multispectral fluorescence medical imaging , the observation system comprising:a beam splitter arranged to receive a light image from an illuminated object, wherein the beam splitter is adapted to split the light image into a first light portion along a first light path and a second light portion along a second light path;a first sensor in the first light path for capturing the first light portion;a second sensor in the second light path for ...

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Номер записи: 83
21-01-2021 дата публикации

A Sample Holder for Imaging a Plurality of Samples

Номер: US20210018740A1
Автор: Alexander AULEHLA, Henning FALK, Lars Hufnagel
Принадлежит: Europaisches Laboratorium fuer Molekularbiologie EMBL

A sample holder comprises one or more elongated sample tubes. The one or more elongated sample tubes are adapted for accommodating a plurality of samples to be imaged at an imaging position. The imaging position is defined by at least one illumination objective lens and at least one detection objective lens of a microscope. A microscope is disclosed, comprising at least one illumination objective lens and at least one detection objective lens, which define an imaging position. The microscope further comprises a sample holder for holding a plurality of samples. The sample holder is moveable with respect to the imaging position. A method for imaging a plurality of samples by means of the microscope is disclosed.

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Номер записи: 84
21-01-2021 дата публикации

Microscope System and Method for Controlling a Microscope System of this Type

Номер: US20210018741A1
Автор: FABRIS Stefan, Huber Stefan, PAN Jiahao, SCHWAB Karin
Принадлежит:

A microscope system and a method for controlling the microscope system have a microscope with several microscope components electrically adjustable and/or activatable via a control apparatus at least one objective, one illumination device, and a camera generating a digital microscopic image, and having a control and display device generating control signals for controlling at least one of the components and displaying the microscopic image; the control and display device is connected to the control apparatus and comprising a display area; the operating element with several operating fields; one or several operating fields configured such that the control and display device, upon selection of an operating field, controls one or several of the components and/or means for modifying settings in the control and display device; upon selection of the operating element as a whole, it becomes modified, via within the display area, regarding its position, its size, shape. 1. A microscope system comprising:a microscope having several microscope components and a control apparatus for electrically adjusting and/or activating said several microscope components;at least one microscope objective, at least one microscope illumination device, and a microscope camera for generating a digital microscopic image;a control and display device serving to generate control signals for controlling at least one of the several adjustable and/or activatable microscope components and for displaying the digital microscopic image, the control and display device being communicatingly connected to the control apparatus and comprising a display area for displaying at least a portion of the digital microscopic image with overlaid a virtual graphical operating element;the operating element comprising several operating fields;at least one of the several operating fields being configured such that upon selection of one of the several operating fields the control and display device, applies control to the ...

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Номер записи: 85
26-01-2017 дата публикации

PIVOTABLE OPTICAL ASSEMBLY FOR A SURGICAL MICROSCOPE, IN PARTICULAR 0°-ASSISTANT'S DEVICE, AND A RETAINING SYSTEM FOR SAID PIVOTABLE OPTICAL ASSEMBLY OF THE SURGICAL MICROSCOPE

Номер: US20170020624A1
Автор: GUENTERT Michael, Schlumpf Curdin
Принадлежит:

The present invention provides a pivotable optical assembly for a surgical microscope, specifically a zero-degree assistant's device, the assembly comprising: a microscope body interface for mounting the assembly on a microscope body; an assistant's module including an interface for an assistant's tube, the assistant's module being pivotable about a pivot axis relative to the microscope body; and a retaining system that blocks pivoting the assistant's module about the pivot axis. The retaining system comprises: a retaining element being moveable from a locking position into a release position, a biasing member forcing the retaining element into the locking position, and a hand-operable control element acting on the retaining element for moving it from the locking position into the release position, as well as a pivotable optical assembly comprising said retaining system according to the invention, whose retaining element, in its blocking position, blocks pivoting the assistant's module about the pivot axis. 1. A retaining system for a pivotable optical assembly of a surgical microscope , the retaining system comprising: a retaining element being moveable from a locking position into a release position , a biasing member forcing the retaining element into the locking position , and a hand-operable control element acting on the retaining element for moving it from the locking position into the release position.2. The retaining system according to claim 1 , wherein the retaining element is a locking bolt being moveable linearly between the locking position and the release position.3. The retaining system according to claim 2 , wherein the locking bolt comprises a beveled locking tip and claim 2 , opposite thereto a biasing end claim 2 , on which the biasing member exerts the biasing force.4. The retaining system according to claim 1 , wherein the retaining element comprises a cam that engages with the control element.5. The retaining system according to claim 4 , ...

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Номер записи: 86
26-01-2017 дата публикации

MICROSPECTROSCOPY DEVICE

Номер: US20170023409A1
Автор: FUJII Akira, Fujioka Michiru, KUSAKA Hiromi, OOURA Takeshi, Sonehara Tsuyoshi, Yamamoto Shuhei
Принадлежит:

With a microspectroscopy device provided with an objective lens with a high numerical aperture, a defocus arises from thermal drift, etc., necessitating auto-focusing. Conventional auto-focus based on through-focus image acquisition takes time, and thus, it cannot be applied to continuous measurement over a long time wherein high-speed sampling is carried out. The present invention addresses this problem by having a defocus-sensing beam that has either defocus or astigmatism fall incident on the objective lens. Since how the image of the spot of the beam for defocus sensing blurs differs depending on the orientation of the defocus, real-time detection of the amount and orientation of defocus becomes possible, and high-speed realtime auto-focus becomes possible. 1. A microspectroscopy device comprising:a light source for outputting an excitation beam for exciting a light emission from a sample;an objective lens for illuminating the sample with the excitation beam;an imaging device for detecting the light emission from the sample excited by the excitation beam;a defocus-sensing beam having a wavelength or an optical axis different from a wavelength or an optical axis of the excitation beam incident on the objective lens;a stage for moving the sample in a direction of an optical axis of the objective lens;a monitoring imaging device for observing a shape of an image formed on the sample by the defocus-sensing beam having passed through the objective lens; anda controller for detecting a direction and an amount of defocus from the shape of the image of the defocus-sensing beam and for sending a feedback control signal so as to move the stage to a focus position of the objective lens,the defocus-sensing beam includes at least two or more defocus-sensing beamsat least one of the at least two or more defocus-sensing beams is focused by the objective lens at a position closer to the objective lens than a position where the excitation light beam is focused by the objective ...

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Номер записи: 87
26-01-2017 дата публикации

ATOMIC FORCE MICROSCOPE MEASURING DEVICE

Номер: US20170023611A1
Автор: GERBER Christoph, MARTIN Sascha, Martinez-Martin David, MUELLER Daniel J.
Принадлежит:

Atomic force microscope measuring device comprising a micro-cantilever and an intensity modulated laser exciting the cantilever, wherein the measuring device comprises an optical microscope, in particular a fluorescence microscope, a confocal microscope, a fluorescence energy transfer (FRET) microscope, a DIC and/or phase contrast microscope, all of those in particular construed as an inverted microscope. 1. Art atomic force microscope measuring device comprising a micro-cantilever and an intensity modulated light source exciting the cantilever , wherein the measuring device comprises an optical microscope.2. The measuring device according to claim 1 , wherein the cantilever is transparent for a wavelength of the visual spectrum.3. The measuring device according to claim 1 , wherein the cantilever has a length in the range of 10 μm to 1000 μm claim 1 , and/or a fundamental resonance frequency in a range of 1 Hz to 10 MHz when immersed in water and/or an oscillation amplitude when excited at resonance in the range of 0.01 nm to 500 nm and/or the exciting light has a wavelength in the range of 350 nm to 750 nm.4. The measuring device according to claim 1 , wherein the exciting light spot is focused on the base of the cantilever.5. The measuring device according to claim 1 , wherein the exciting light spot is smaller than 100 μm in diameter.6. The measuring device according to claim 1 , wherein said optical microscope is at least one selected from the group consisting of a fluorescence microscope claim 1 , a confocal microscope claim 1 , a fluorescence energy transfer (FRET) microscope claim 1 , a DIC and/or phase contrast microscope or a Raman spectrometer all of those in particular construed as an inverted microscope.7. The measuring device according to claim 3 , wherein the cantilever has a length in the size of 10 μm to 500 μm and/or a fundamental resonance frequency in the range of 1 kHz to 2000 kHz claim 3 , when immersed in water and/or an oscillation amplitude ...

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Номер записи: 88
28-01-2016 дата публикации

SAMPLE OBSERVATION METHOD AND SAMPLE OBSERVATION DEVICE

Номер: US20160025959A1
Автор: Suzuki Yoshimasa
Принадлежит: OLYMPUS CORPORATION

A sample observation method includes an acquisition of for acquiring an electronic image of a sample, and a subtraction step of subtracting a DC component from a signal of the electronic image, and the acquisition step is performed in a state of bright-field observation, the electronic image at the subtraction step is an image acquired in a first predetermined state, and in the first predetermined state, at least a position of the sample and a in-focus position of an image forming optical system are different. A sample observation device includes a light source, an illumination optical system, an image forming optical system, an image-pickup device, and an image processing device, and the illumination optical system is disposed so as to irradiate a sample with illumination light from the light source, the image forming optical system is disposed so that light from the sample is incident thereon and an optical image of the sample is formed, the image-pickup device is disposed at a position of the optical image, and the image processing device is configured to implement the aforementioned sample observation method. 3. The sample observation method according to claim 1 , further comprising:an amplification step after the subtraction step, whereinat the amplification step, a signal of an electronic image subjected to the subtraction step is amplified.6. The sample observation method according to claim 1 , whereina position of the sample is changed relative to a in-focus position of the image forming optical system a plurality of times,at each position of the sample after changing, the acquisition step and the subtraction step are performed, and thereby a plurality of electronic images are generated after the subtraction step, andthe plurality of electronic images generated are added.7. The sample observation method according to claim 6 , whereinbefore addition, a part with highest contrast in each of the plurality of electronic images is extracted, andaddition is ...

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Номер записи: 89
26-01-2017 дата публикации

AN OPTICAL ARRANGEMENT AND METHOD FOR IMAGING A SAMPLE

Номер: US20170023784A1
Автор: Hufnagel Lars, Norlin Nils, Quintas Glasner de Medeiros Gustavo
Принадлежит:

An optical arrangement for imaging a sample is disclosed. The optical arrangement comprises at least one first objective lens and at least one second objective lens, at least one illumination source for producing an illumination beam, detector for imaging radiation from the sample, and at least one mirror for reflecting the radiation from one of the first objective lens or the second objective lens into the detector. The at least one mirror is double-sided and dependent on the illumination beam at the other one of the first objective lens and the second objective lens. 111.-. (canceled)12. An optical arrangement for imaging a sample comprising:at least one first objective lens and at least one second objective lens;at least one illumination source for producing an illumination beam;a detector for imaging radiation from the sample; andat least one movable mirror for at least one of reflecting the illumination beam towards one of the first objective lens or the second objective lens, or reflecting the radiation from the other one of the first objective lens or the second objective lens to the detector, wherein, depending on the position of the selected one of the at least one movable mirror, the illumination beam is directed through one of the first objective lens or the second objective lens, and the radiation is directed through the other one of the first objective lens or the second objective lens.13. The optical arrangement of claim 12 , wherein the at least one movable mirror is translatable or rotatable.14. The optical arrangement of claim 12 , wherein the at least one movable mirror is double-sided.15. The optical arrangement of claim 12 , further comprising at least two further movable mirrors and wherein the reflected radiation can be directed into one of the at least two further movable mirrors.16. The optical arrangement of claim 12 , further comprising an image processor connected to the detector.17. The optical arrangement of claim 12 , wherein the sample ...

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Номер записи: 90
28-01-2021 дата публикации

IMAGE PICKUP DEVICE, MICROSCOPE IMAGE PICKUP SYSTEM, AND ENDOSCOPE IMAGE PICKUP SYSTEM

Номер: US20210022591A1
Автор: NAGAE Satoshi
Принадлежит: SONY CORPORATION

A medical imaging device in accordance with the present application includes a color separation prism, a fluorescence image sensor, a visible light image sensor, and a bandpass filter. The color separation prism splits light into first light belonging to a visible light wavelength band and second light belonging to a fluorescence wavelength band. The fluorescence image sensor is provided at an output side of the color separation prism and is configured to image at least part of the second light belonging to the fluorescence wavelength band separated by the dichroic film. The visible light image sensor is provided at the output side of the color separation prism and is configured to image at least part of the first light belonging to the visible light wavelength band separated by the dichroic film. The bandpass filter is disposed between the color separation prism and the fluorescence image sensor. 1. A medical imaging device comprising:a color separation prism that has a dichroic film configured to split light into first light belonging to a visible light wavelength band and second light belonging to a fluorescence wavelength band;a fluorescence image sensor that is provided at an output side of the color separation prism and that is configured to image at least part of the second light belonging to the fluorescence wavelength band separated by the dichroic film;a visible light image sensor that is provided at the output side of the color separation prism and that is configured to image at least part of the first light belonging to the visible light wavelength band separated by the dichroic film; anda bandpass filter that is disposed between the color separation prism and the fluorescence image sensor,wherein the fluorescence image sensor and the visible light image sensor are arranged such that an optical path difference between an optical path length of a fluorescence optical path for the second light imaged on the fluorescence image sensor via the color ...

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Номер записи: 91
25-01-2018 дата публикации

METHOD AND SCANNING FLUORESCENCE MICROSCOPE FOR MULTI-DIMENSIONAL HIGH-RESOLUTION IMAGING A STRUCTURE OR A PATH OF A PARTICLE IN A SAMPLE

Номер: US20180024063A1
Автор: Egner Alexander, Geisler Claudia, Krueger Jennifer-Rose
Принадлежит:

For multi-dimensional high-resolution imaging a structure marked with fluorescence markers, fluorescence enabling light is focused to illuminate a measurement area in a sample. A partial area of the measurement area is subjected to fluorescence inhibiting light. The partial area omits a center of the measurement area in that an intensity distribution of the fluorescence inhibiting light comprises a line-shaped intensity minimum. A minimal extension of the intensity minimum in a direction through the center area is by a factor k≧2 smaller than a diameter of the measurement area in said direction. Without spatial resolution, fluorescence light emitted out of the measurement area is measured for a plurality of consecutive angle positions of the intensity minimum about the center, while the measurement area, for each angle position, is subjected to the fluorescence enabling light. A value of the measured fluorescence light is assigned to the position of the center. 1. A method of multi-dimensional high-resolution imaging a structure of a sample , which is marked with fluorescence markers , the method comprising:focusing fluorescence enabling light into the sample, the focused fluorescence enabling light illuminating a measurement area in the sample;subjecting a partial area of the measurement area to fluorescence inhibiting light, wherein the partial area omits a center of the measurement area in that an intensity distribution of the fluorescence inhibiting light comprises a line-shaped or plane-shaped intensity minimum extending across the center of the measurement area, and wherein a minimal extension of the intensity minimum in a direction through the center of the measurement area is by a factor k≧2 smaller than a diameter of the measurement area in said direction;measuring fluorescence light emitted from the sample out of the measurement area without spatial resolution within the measurement area, wherein the fluorescence light emitted out of the measurement area ...

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Номер записи: 92
25-01-2018 дата публикации

MAGNIFYING OBSERVATION APPARATUS

Номер: US20180024339A1
Автор: Inomata Masahiro, MATSUMURA Shingo
Принадлежит: KEYENCE CORPORATION

An emitting section of a light projecting section is provided to surround an optical axis of an objective lens of a lens unit. The optical axis of the emitting section is substantially the same as the optical axis of the objective lens. Ring illumination is irradiated on the observation target from the emitting section and light from the observation target is received by an imaging section via the objective lens, whereby first original image data is generated. Directional illumination is irradiated on the observation target from the emitting section and the light from the observation target is received by the imaging section via the objective lens, whereby second original image data is generated. Image data for display indicating an image of the observation target that should be obtained when it is assumed that light in a specific emitting direction is irradiated on the observation target is generated. 1. A magnifying observation apparatus comprising:a stage including a placement surface on which an observation target is placed;a lens unit including an objective lens;a light projecting unit including an emitting section having an optical axis substantially same as an optical axis of the objective lens and provided to surround the optical axis of the objective lens, the light projecting unit selectively irradiating, on the observation target placed on the stage, light in a first emitting direction and light in a second emitting direction different from the first emitting direction;an imaging section configured to receive light from the observation target via the objective lens and generate first or second original image data indicating an image of the observation target; anda data generating section configured to generate image data for display indicating an image of the observation target that should be obtained when it is assumed that light in a specific emitting direction designated by a user is irradiated on the observation target, whereinat a first light ...

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Номер записи: 93
25-01-2018 дата публикации

MAGNIFYING OBSERVATION APPARATUS

Номер: US20180024340A1
Автор: Inomata Masahiro, MATSUMURA Shingo
Принадлежит: KEYENCE CORPORATION

Lights in a plurality of emitting directions different from one another are selectively irradiated on the observation target from a light projecting section. A plurality of image data indicating images of the observation target at the time when the lights in the plurality of emitting directions are respectively irradiated on the observation target are generated by an imaging section. An imaginary emitting direction of light is designated on the basis of operation of an operation section by the user. Image data for display indicating an image of the observation target that should be obtained when it is assumed that the light in the designated emitting direction is irradiated on the observation target is generated on the basis of the designated emitting direction and the plurality of image data generated by the imaging section. The image based on the generated image data for display is displayed on the display section. 1. A magnifying observation apparatus comprising:a stage on which an observation target is placed;a light projecting device configured to selectively irradiate lights in a plurality of emitting directions different from one another on the observation target placed on the stage;an imaging section configured to receive light from the observation target and respectively generate a plurality of image data indicating images of the observation target at times when the lights in the plurality of emitting directions are respectively irradiated on the observation target;a designating section configured to designate an imaginary emitting direction of light on the basis of operation by a user;a data generating section configured to generate, on the basis of the emitting direction designated by the designating section and the plurality of image data generated by the imaging section, image data for display indicating an image of the observation target that should be obtained when it is assumed that the light in the designated emitting direction is irradiated on the ...

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Номер записи: 94
25-01-2018 дата публикации

AUGMENTED STEREOSCOPIC MICROSCOPY

Номер: US20180024341A1
Автор: GAINER CHRISTIAN, Romanowski Marek, WATSON JEFFREY
Принадлежит:

Various examples are provided related to imaging with augmented stereoscopic microscopes. In one example, an augmented stereoscopic microscope includes an objective lens that can simultaneously receive near infrared (NIR) images and visible bright-field images of an examined object and an augmentation module. The augmentation module can separate the NIR images from the visible bright-field images for processing by an image processing unit to produce synthetic images using the NIR images and combine the synthetic images with the visible bright-field images to form co-registered augmented images that are directed to an eyepiece of the augmented stereoscopic microscope. In another example, a method includes obtaining a NIR image of an examined object; generating a synthetic image using the NIR image; combining the synthetic image with a real-time visual image of the examined object to form an augmented image; and directing the augmented image to an eyepiece of an augmented stereoscopic microscope. 1. An augmented stereoscopic microscope , comprising:an objective lens configured to simultaneously receive near infrared (NIR) images and visible bright-field images of an examined object; and separate the NIR images from the visible bright-field images for processing by an image processing unit to produce synthetic images based upon the NIR images; and', 'combine the synthetic images with the visible bright-field images to form co-registered augmented images that are directed to an eyepiece of the augmented stereoscopic microscope., 'an augmentation module configured to2. The augmented stereoscopic microscope of claim 1 , wherein the augmentation module comprises a beamsplitter configured to combine the synthetic images with the visible bright-field images to form the co-registered augmented images.3. The augmented stereoscopic microscope of claim 2 , wherein combining the synthetic images with the visible bright-field images comprises projecting the synthetic images onto ...

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Номер записи: 95
25-01-2018 дата публикации

APPARATUS AND METHOD FOR CONTROLLING A PLURALITY OF OPTICAL TRAPS

Номер: US20180024342A1
Автор: Biebricher Andreas Sebastian, Candelli Andrea, Heller Iddo, Laurens Niels, Peterman Erwin Johannes Gerard, Wuite Gijs Jan Lodewijk
Принадлежит: Stichting Vu

The present disclosure relates to an apparatus () and method for controlling a plurality of simultaneously active optical traps (OT, OT, OT). In one method, trapping beams (TB, TB, TB) are provided and redirected for individually controlling a respective position (X,Y) of optical traps (OT, OT, OT) formed by focusing of the redirected trapping beams in a sample volume (SV). Light (L,L) from the sample volume (SV) corresponding to the optical traps is received. A path of a detector beam (AB) is overlapped with one of the trapping beams (TB), wherein the detector beam has a distinct wavelength (λA) from that of the overlapping trapping beam (TB). In one channel, the light from the sample volume is filtered according to wavelength, and only the filtered light having the wavelength (λA) of the detector beam (AB) is measured. 116.-. (canceled)17. An apparatus for controlling a plurality of optical traps , the apparatus comprisingtrapping beam optics configured to provide a plurality of simultaneously active trapping beams,controllable mirrors configured to receive and redirect the trapping beams for individually controlling a respective position of a plurality of simultaneously active optical traps formed by focusing of the redirected trapping beams in a sample volume;light detectors configured to receive light from the sample volume corresponding to the optical traps;detector beam optics configured to overlap a path of a detector beam with one of the trapping beams, wherein the detector beam has a distinct wavelength from that of the overlapping trapping beam; anda wavelength filter disposed in a light path between the sample volume and a first light detector, and configured to pass light with the wavelength of the detector beam to the first light detector; whereinthe trapping beam optics are configured to provide at least three simultaneously active trapping beams, wherein a first of the trapping beams is provided with a first polarization, and the other trapping beams ...

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Номер записи: 96
25-01-2018 дата публикации

Microscope

Номер: US20180024344A1
Автор: Atsushi Ueda
Принадлежит: Shimadzu Corp

A microscope equipped with an imaging device used for the positioning of a sample, the microscope capable of removing distortion and blind spots from an image generated by the imaging device and reducing cost by using a commercially available imaging device. A sample holder for holding a sample; a measurement light source for irradiating the sample held by the sample holder with irradiation light; a focusing optical element for focusing measurement light derived from the irradiation light transmitted through or reflected from the sample; a detection unit for detecting the measurement light focused by the focusing optical element; an image capture device for capturing the image of the sample; and an objective optical system switching unit arranged to switch either the focusing optical element or the image capture device with the other to a position facing the sample.

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Номер записи: 97
25-01-2018 дата публикации

MAGNIFYING OBSERVATION APPARATUS

Номер: US20180024346A1
Автор: Inomata Masahiro, MATSUMURA Shingo
Принадлежит: KEYENCE CORPORATION

Light in a first emitting direction is irradiated on an observation target from a light projecting section and light from the observation target is received by an imaging section via an objective lens while a focal position of the light is changed in an optical axis direction of the objective lens, whereby a plurality of first original image data are generated in a plurality of focal positions. Light in a second emitting direction different from the first emitting direction is irradiated on the observation target from the light projecting section, whereby a plurality of second original image data are generated. First and second focused image data are respectively acquired on the basis of the pluralities of first and second original image data. 1. A magnifying observation apparatus comprising:an objective lens;a light projecting section configured to selectively irradiate, on an observation target, lights from first and second emitting directions different from each other;an imaging section configured to receive light from the observation target via the objective lens and generate first and second original image data indicating an image of the observation target;a focal-position changing section configured to change a focal position of the light passed through the objective lens to an optical axis direction of the objective lens relatively to the observation target; anda data generating section configured to generate image data for display indicating an image of the observation target that should be obtained when it is assumed that light in a specific emitting direction designated by a user is irradiated on the observation target,wherein, at a first light irradiation time, the light projecting section irradiates the light in the first emitting direction on the observation target and the imaging section generates a plurality of the first original image data in a different plurality of the focal positions changed by the focal-position changing section, at a second ...

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Номер записи: 98
24-01-2019 дата публикации

PHOTONIC SUPERLATTICE-BASED DEVICES AND COMPOSITIONS FOR USE IN LUMINESCENT IMAGING, AND METHODS OF USING THE SAME

Номер: US20190024163A1
Автор: Dehlinger Dietrich, Topolancik Juraj, Zhong Cheng Frank
Принадлежит:

Under one aspect, a device is provided for use in luminescent imaging. The device can include a photonic superlattice including a first material, the first material having a first refractive index. The first material can include first and second major surfaces and first and second pluralities of features defined through at least one of the first and second major surfaces, the features of the first plurality differing in at least one characteristic from the features of the second plurality. The photonic superlattice can support propagation of a first wavelength and a second wavelength approximately at a first angle out of the photonic superlattice, the first and second wavelengths being separated from one another by a first non-propagating wavelength that does not selectively propagate at the first angle out of the photonic superlattice. 1. A device comprising:a photonic superlattice comprising a surface having wells of different sizes, the photonic superlattice supporting propagation of a first wavelength and a second wavelength, the photonic superlattice inhibiting propagation of a third wavelength between the first and second wavelengths; andan optical component facing the surface, the optical component receiving first luminescence at the first wavelength at an angle, the optical component receiving second luminescence at the second wavelength at approximately the angle.2. The device of claim 1 , wherein the angle is approximately normal to the surface.3. The device of claim 1 , wherein the photonic superlattice comprises a first material that comprises the wells claim 1 , the device further comprising a second material in contact with the first material.4. The device of claim 3 , wherein the second material has a higher refractive index than the first material.5. The device of claim 3 , further comprising a third material in contact with at least the second material claim 3 , the third material comprising first and second luminophores claim 3 , the first ...

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Номер записи: 99
10-02-2022 дата публикации

METHOD, COMPUTER PROGRAM, AND APPARATUS FOR ADAPTING AN ESTIMATOR FOR USE IN A MICROSCOPE

Номер: US20220042914A1
Автор: SCHMIDT Roman
Принадлежит:

The present invention is related to a method, a computer program, and apparatus for adapting an estimator for use in a microscope for estimating a position of an emitter in a sample based on a method, in which the sample is illuminated with light at one or more sets of probe positions and fluorescence photons are acquired for the sets of probe positions. The invention is further related to a microscope, which makes use of such a method or apparatus. The sample is illuminated with light at one or more sets of probe positions and fluorescence photons are acquired for the sets of probe positions. Photon counts of the acquired photons are then added to vectors of photon counts or sums of photon counts are determined for the sets of probe positions. A value representative of background noise is determined and used for adapting the estimator in real-time. 1. A method for adapting an estimator for use in a microscope for estimating a position of an emitter in a sample based on a method , in which the sample is illuminated with light at one or more sets of probe positions (P) and fluorescence photons are acquired for the sets of probe positions (P) , comprising:{'sub': 'i', 'illuminating the sample with light at one or more sets of probe positions (P);'}acquiring fluorescence photons for the sets of probe positions;adding photon counts of the acquired photons to vectors of photon counts or sums of photon counts for the sets of probe positions;determining a value representative of background noise from the vectors of photon counts or the sums of photon counts; andadapting the estimator in real-time using the value representative of background noise.2. The method according to claim 1 , wherein for determining a value representative of background noise only sets of probe positions are evaluated claim 1 , for which no fluorescence from an emitter is detected.3. The method according to claim 2 , wherein for determining whether fluorescence from an emitter is detected claim 2 , ...

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Номер записи: 100