18-01-2018 дата публикации
Номер: US20180018498A1
Принадлежит:
Methods, storage mediums, and systems for image data processing are provided. Embodiments for the methods, storage mediums, and systems include configurations to perform one or more of the following steps: background signal measurement, particle identification using classification dye emission and cluster rejection, inter-image alignment, inter-image particle correlation, fluorescence integration of reporter emission, and image plane normalization. 17-. (canceled)8. A system , comprising: selecting a first set of one or more optical filters corresponding to a first wavelength band;', 'illuminating the particles through the first set of optical filters;', 'selecting a second set of one or more optical filters corresponding to a second wavelength band; and', 'illuminating the particles through the second set of optical filters; and, 'an imaging subsystem configured to image, at different wavelength bands, particles disposed within the imaging subsystem, wherein the imaging comprises store data acquired for multiple images of the particles, and wherein particular images of the multiple images include spots corresponding to the particles, wherein a first image of the particular images corresponds to the first set of optical filters, wherein a second image of the particular images corresponds to the second set of optical filters;', 'create a first composite image of the multiple images, wherein the first composite image includes first composite spots corresponding to the particles, the first composite spots having a first amount of misalignment from the spots in the particular images; and', 'modify coordinates of at least one of the multiple images such that a second composite image based on the modified coordinates includes second composite spots having a second, smaller amount of misalignment from the spots in the multiple images., 'a data processing subsystem configured to9. The system of claim 8 , wherein the first set of optical filters corresponds to a plurality of ...
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