16-05-2023 дата публикации
Номер: CN116121424A
Принадлежит:
The invention discloses a method for visual, isothermal and signal-on detection of helicobacter pylori pathogens based on trident neck ring structure DNA (deoxyribonucleic acid) and a CRISPR/Cas12a (clustered regularly interspaced short palindromic repeats/CRISPR associated 12a) system, and belongs to the technical field of nucleic acid detection. A helicobacter pylori pathogen is taken as a template, nucleic acid of the helicobacter pylori pathogen is subjected to extraction and recombinase polymerase amplification, then incubated with a CRISPR/Cas12a system and trifurcate neck ring structure DNA (deoxyribonucleic acid) taken as a reporter probe, and finally reacted with hemin and a color developing agent, and detection is carried out by naked eyes or through an ultraviolet-visible light absorption spectrum. The method realizes simple, rapid, sensitive, specific and accurate detection of helicobacter pylori, does not need complex operation steps and instruments and equipment, and has a ...
Подробнее