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Небесная энциклопедия

Космические корабли и станции, автоматические КА и методы их проектирования, бортовые комплексы управления, системы и средства жизнеобеспечения, особенности технологии производства ракетно-космических систем

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Мониторинг СМИ

Мониторинг СМИ и социальных сетей. Сканирование интернета, новостных сайтов, специализированных контентных площадок на базе мессенджеров. Гибкие настройки фильтров и первоначальных источников.

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Поддерживает ввод нескольких поисковых фраз (по одной на строку). При поиске обеспечивает поддержку морфологии русского и английского языка
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Применить Всего найдено 7. Отображено 7.
01-06-2011 дата публикации

Hairpin type DNA modified gold colloid nano particles and synthesizing method thereof

Номер: CN0102080131A
Автор: HUIFANG CUI, YULONG SUN, YUHAN CUI, CUI HUIFANG, SUN YULONG, CUI YUHAN
Принадлежит:

The invention discloses hairpin type DNA (hpDNA) modified gold colloid nano particles and a synthesizing method thereof, which relate to the technical field of biological nanomaterial synthesis. The method is characterized in that: an hpDNA (HS-hpDNA) one end part of which is connected with a sulfydryl and an ssDNA (HS-ssDNA) one end part of which is connected with a sulfydryl are highly modified onto the surfaces of the gold colloid nano particles together through gold-disulfide bonds; in order to inhibit the formation of dimers between different hpDNAs, a section of DNA sequence (CpDNA) which is complemented with a section of sequence at the free end of the hpDNA is introduced into a reaction system together with the HS-hpDNA; and after the synthetic reaction and ageing, the reaction product is repeatedly centrifuged and dispersed by a high-speed centrifuge at a low temperature, and the liquid of the final product is preserved at a low temperature. The oligonucleotide highly modified ...

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Номер записи: 1
11-12-1996 дата публикации

Preparing reagent grade sulfosalicylic acid and its sodium salt by industrial product epuration

Номер: CN0001137517A
Автор: JING ZHU, HUIFANG CUI, FU JINGGUO, ZHU JING, CUI HUIFANG
Принадлежит:

Reagent-grade sulfosalicylic acid is prepared as follows: the industrial product sulfosalicylic acid is dissolved, cooled, crystalized and centrifuged, and its mother liquor (A) is recovered. The crystalline product is dissolved in deionized water, active carbon is added into the solution, then filtered, concentrated, cooled, crystallized, separated by copper centrifuge, crystallized, dried, and its mother liquor (B) is recovered. Active carbon is added into the mother liquor (A+B) mixture, stirred and filtered, then NaOH solution is added into the obtained filtrate to make pH 2-3, the filtrate is concentrated, crystallized, recrystallized, centrifuged, crystallized and dried. up to 99.18% sulfosalicylic acid is obtained.

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Номер записи: 2
28-07-2023 дата публикации

Dialysis tube fixing device and fixing method thereof

Номер: CN116492572A
Автор: CUI HUIFANG, TANG XIAOYANG, GE JIANJIAN, TANG WEIGANG, GONG LIFENG
Принадлежит:

The invention discloses a dialysis catheter fixing device and a fixing method thereof, and belongs to the technical field of dialysis catheters.The dialysis catheter fixing device comprises a shell, a sliding plate installed on the shell, a first dialysis catheter installed on the shell and a second dialysis catheter restrained by the sliding plate, four sliding restraining blocks distributed in a rectangular array mode are installed on the side edge of the sliding plate, and four tension springs are fixed to the side face, close to the arc-shaped face, of the sliding plate. When the dialysis tube fixing device is used, the second dialysis tube can move by a certain distance when the second dialysis tube is pulled, and the second dialysis tube is reset when the second dialysis tube does not need to be pulled, so that the problem that the connectivity of the first dialysis tube and the second dialysis tube is affected due to the fact that the second dialysis tube is accidentally stressed ...

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Номер записи: 3
19-08-2015 дата публикации

DNA aptamer of small cell lung cancer marker gastrin releasing peptide precursor polypeptide fragment

Номер: CN104845975A
Автор: CUI HUIFANG, LI YAJUN, WANG JIA, LI XIAOJIA
Принадлежит:

The invention discloses a DNA aptamer sequence of a small cell lung cancer marker gastrin releasing peptide precursor polypeptide fragment Pro-GRP31-98, and relates to the technical field of chemicobiology. The DNA aptamer sequence is obtained by screening from a single-chain DNA library through an SELEX (Systematic Evolution of Ligands by Exponential Enrichment) screening method based on an affinity magnetic bead separation method, then carrying out PCR amplification on the screened DNA aptamer library, then separating a DNA aptamer contained in the DNA aptamer library through a gene clone technology by taking escherichia coli as a recipient cell and finally sequencing various DNA aptamers through a sequencing technology. An experimental result indicates that the DNA aptamer sequence disclosed by the invention is combined with a gastrin releasing peptide precursor fragment at high affinity and high specificity.

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Номер записи: 4
29-06-2011 дата публикации

Thin-layer chromatography detection method of elecampane in fukant pills

Номер: CN0102106947A
Автор: DUAN GUISHENG, ZHANG YANHONG, FENG JINHE, WEI CHAOYANG, CUI HUIFANG, GUISHENG DUAN, YANHONG ZHANG, JINHE FENG, CHAOYANG WEI, HUIFANG CUI
Принадлежит:

The invention discloses a thin-layer chromatography detection method of elecampane in fukant pills, comprising the following steps: control sample solution preparation, test sample solution preparation and negative control solution preparation and chromatograph recognition: absorbing the solutions prepared in step (1), step (2) and step (3) on a silica gel plate for applying samples, and developing in the developer; spraying 5% vanillic aldehyde sulfuric acid solution, and heating at the temperature of 90-110 DEG C until spots develop; and carrying out comparative analysis on the same Rf values of the spots according to the test sample solution prepared in the step (2), the negative control solution prepared in the step (3), and the reference solution prepared in the step (1). The method provided by the invention has the beneficial effects of low price, strong feasibility and good repeatability, is simple to operate and is beneficial for popularization.

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Номер записи: 5
29-06-2011 дата публикации

Thin-layer chromatography for Chinese angelica in fukang pills

Номер: CN0102106946A
Автор: FENG JINHE, ZHANG YANHONG, DUAN GUISHENG, WEI CHAOYANG, CUI HUIFANG, JINHE FENG, YANHONG ZHANG, GUISHENG DUAN, CHAOYANG WEI, HUIFANG CUI
Принадлежит:

The invention discloses a thin-layer chromatography for Chinese angelica in fukang pills. The thin-layer chromatography is characterized by being operated by the following steps of: the preparation of solution of a reference substance; the preparation of sample solution; the preparation of negative control solution; and chromatographic recognition, namely dripping the solution prepared in step (1), (2) and (3) on a silica gel plate to obtain a sample, developing in a developing agent, air-drying, placing under an ultraviolet lamp with the wavelength of 365nm to view bright blue white fluorescence spots, and performing comparative analysis on the part with the same Rf value of the spots of the sample solution prepared in step (2), the negative control solution prepared in step (3) and the solution of reference substance prepared in step (1). The thin-layer chromatography is simple in operation, strong in feasibility, good in repeatability and favorable for promotion.

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Номер записи: 6
16-05-2023 дата публикации

Method for visually detecting helicobacter pylori

Номер: CN116121424A
Автор: CUI HUIFANG, NIU CHANGBIN, SONG XIAOJIE, LYU QIYAN
Принадлежит:

The invention discloses a method for visual, isothermal and signal-on detection of helicobacter pylori pathogens based on trident neck ring structure DNA (deoxyribonucleic acid) and a CRISPR/Cas12a (clustered regularly interspaced short palindromic repeats/CRISPR associated 12a) system, and belongs to the technical field of nucleic acid detection. A helicobacter pylori pathogen is taken as a template, nucleic acid of the helicobacter pylori pathogen is subjected to extraction and recombinase polymerase amplification, then incubated with a CRISPR/Cas12a system and trifurcate neck ring structure DNA (deoxyribonucleic acid) taken as a reporter probe, and finally reacted with hemin and a color developing agent, and detection is carried out by naked eyes or through an ultraviolet-visible light absorption spectrum. The method realizes simple, rapid, sensitive, specific and accurate detection of helicobacter pylori, does not need complex operation steps and instruments and equipment, and has a ...

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Номер записи: 7