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Небесная энциклопедия

Космические корабли и станции, автоматические КА и методы их проектирования, бортовые комплексы управления, системы и средства жизнеобеспечения, особенности технологии производства ракетно-космических систем

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Применить Всего найдено 4. Отображено 4.
01-06-2011 дата публикации

Hairpin type DNA modified gold colloid nano particles and synthesizing method thereof

Номер: CN0102080131A
Автор: HUIFANG CUI, YULONG SUN, YUHAN CUI, CUI HUIFANG, SUN YULONG, CUI YUHAN
Принадлежит:

The invention discloses hairpin type DNA (hpDNA) modified gold colloid nano particles and a synthesizing method thereof, which relate to the technical field of biological nanomaterial synthesis. The method is characterized in that: an hpDNA (HS-hpDNA) one end part of which is connected with a sulfydryl and an ssDNA (HS-ssDNA) one end part of which is connected with a sulfydryl are highly modified onto the surfaces of the gold colloid nano particles together through gold-disulfide bonds; in order to inhibit the formation of dimers between different hpDNAs, a section of DNA sequence (CpDNA) which is complemented with a section of sequence at the free end of the hpDNA is introduced into a reaction system together with the HS-hpDNA; and after the synthetic reaction and ageing, the reaction product is repeatedly centrifuged and dispersed by a high-speed centrifuge at a low temperature, and the liquid of the final product is preserved at a low temperature. The oligonucleotide highly modified ...

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Номер записи: 1
11-12-1996 дата публикации

Preparing reagent grade sulfosalicylic acid and its sodium salt by industrial product epuration

Номер: CN0001137517A
Автор: JING ZHU, HUIFANG CUI, FU JINGGUO, ZHU JING, CUI HUIFANG
Принадлежит:

Reagent-grade sulfosalicylic acid is prepared as follows: the industrial product sulfosalicylic acid is dissolved, cooled, crystalized and centrifuged, and its mother liquor (A) is recovered. The crystalline product is dissolved in deionized water, active carbon is added into the solution, then filtered, concentrated, cooled, crystallized, separated by copper centrifuge, crystallized, dried, and its mother liquor (B) is recovered. Active carbon is added into the mother liquor (A+B) mixture, stirred and filtered, then NaOH solution is added into the obtained filtrate to make pH 2-3, the filtrate is concentrated, crystallized, recrystallized, centrifuged, crystallized and dried. up to 99.18% sulfosalicylic acid is obtained.

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Номер записи: 2
29-06-2011 дата публикации

Thin-layer chromatography detection method of elecampane in fukant pills

Номер: CN0102106947A
Автор: DUAN GUISHENG, ZHANG YANHONG, FENG JINHE, WEI CHAOYANG, CUI HUIFANG, GUISHENG DUAN, YANHONG ZHANG, JINHE FENG, CHAOYANG WEI, HUIFANG CUI
Принадлежит:

The invention discloses a thin-layer chromatography detection method of elecampane in fukant pills, comprising the following steps: control sample solution preparation, test sample solution preparation and negative control solution preparation and chromatograph recognition: absorbing the solutions prepared in step (1), step (2) and step (3) on a silica gel plate for applying samples, and developing in the developer; spraying 5% vanillic aldehyde sulfuric acid solution, and heating at the temperature of 90-110 DEG C until spots develop; and carrying out comparative analysis on the same Rf values of the spots according to the test sample solution prepared in the step (2), the negative control solution prepared in the step (3), and the reference solution prepared in the step (1). The method provided by the invention has the beneficial effects of low price, strong feasibility and good repeatability, is simple to operate and is beneficial for popularization.

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Номер записи: 3
29-06-2011 дата публикации

Thin-layer chromatography for Chinese angelica in fukang pills

Номер: CN0102106946A
Автор: FENG JINHE, ZHANG YANHONG, DUAN GUISHENG, WEI CHAOYANG, CUI HUIFANG, JINHE FENG, YANHONG ZHANG, GUISHENG DUAN, CHAOYANG WEI, HUIFANG CUI
Принадлежит:

The invention discloses a thin-layer chromatography for Chinese angelica in fukang pills. The thin-layer chromatography is characterized by being operated by the following steps of: the preparation of solution of a reference substance; the preparation of sample solution; the preparation of negative control solution; and chromatographic recognition, namely dripping the solution prepared in step (1), (2) and (3) on a silica gel plate to obtain a sample, developing in a developing agent, air-drying, placing under an ultraviolet lamp with the wavelength of 365nm to view bright blue white fluorescence spots, and performing comparative analysis on the part with the same Rf value of the spots of the sample solution prepared in step (2), the negative control solution prepared in step (3) and the solution of reference substance prepared in step (1). The thin-layer chromatography is simple in operation, strong in feasibility, good in repeatability and favorable for promotion.

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Номер записи: 4