04-04-2023 дата публикации
Номер: CN115902014A
Принадлежит:
The invention discloses a marigold alcohol extract characteristic chromatogram construction method, which comprises: 1) preparing a reference substance solution, respectively taking a certain amount of ellagic acid, quercetagetin, 6-hydroxykaempferol, quercetin and tagetin, adding a methanol aqueous solution, dissolving, carrying out volume metering, and filtering to obtain a filtrate as a reference substance mixed solution; 2) preparation of a test solution: taking dried marigold flowers, and crushing the dried marigold flowers to obtain a test solution; the method comprises the following steps: taking a certain amount of a test sample, adding a methanol aqueous solution solvent for extraction, cooling, fixing the volume, and filtering to obtain filtrate, namely a test sample solution; 3) performing UPLC analysis on the reference substance: injecting the reference substance solution in the step 1) into an ultra-high performance liquid chromatography ultraviolet detector, and detecting ...
Подробнее