23-06-2023 дата публикации
Номер: CN116286575A
Принадлежит:
The invention discloses a method for efficiently expressing raw starch alpha-amylase by using bacillus subtilis, and belongs to the field of genetic engineering and bioengineering. According to the bacillus subtilis recombinant bacteria, pBHVY is used as a carrier skeleton, a raw starch alpha-amylase gene derived from Pontibacillus sp.ZY is used as a target gene, bacillus subtilis WB600 is used as an expression host, a promoter is modified, a signal peptide is screened, an RBS sequence is optimized, and 3-L tank fermentation feeding process optimization is carried out on the obtained recombinant bacteria. The raw starch alpha-amylase can be efficiently expressed by using the bacillus subtilis recombinant strain, the bacillus subtilis recombinant strain is used as a production strain, shake flask fermentation is performed for 48 hours, the enzyme activity of the raw starch alpha-amylase in fermentation supernatant is 4824.2 U/mL, and the enzyme activity of the extracellular starch alpha-amylase ...
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