Cationic lipid compound, preparation method and application thereof, and mRNA delivery system

The invention relates to a cationic lipid compound, a preparation method and application thereof, and an mRNA delivery system. The purpose of the present invention is to solve the problem of the need to develop cationic lipids having high delivery efficiency and excellent immune activation characteristics. Wherein the structure of the cationic lipid compound is as shown in the following formula (I): in the formula (I), X is O or N; n is 2-4; m is 2-4; a is 0 or 1; r1 is a chain structure containing tertiary amine; r2 is a straight-chain fatty acyl group or a chain fatty acyl group with a branched chain; and R3 is a chain fatty acyl group with a branched chain. The invention is mainly used for developing the cationic lipid compound, and the cationic lipid compound is used for preparing an mRNA delivery system and has the characteristics of high delivery efficiency, organ targeted delivery and excellent immune activation characteristic.

轮胎修补工具

ORGANIC COMPOUND AND ELECTRONIC DEVICE AND ELECTRONIC DEVICE COMPRISING

The invention relates to an organic compound, and an electronic element and an electronic device comprising the same. The structural formula of the organic compound is as shown in the formula 1, and the performance of the device can be remarkably improved when the organic compound is applied to the organic electroluminescent device.

Method for reducing total nitrogen in aquaculture water body by utilizing slow-release carbon source

The invention discloses a method for reducing total nitrogen in an aquaculture water body by using a slow-release carbon source, and belongs to the technical field of aquaculture tail water treatment.The method mainly comprises the steps that a certain amount of polyhydroxyalkanoate particles are directly added into the aquaculture water body for denitrification treatment in the aquaculture process, and the total nitrogen content of the aquaculture water body is determined by measuring the total nitrogen index of the water body; it is verified that the particles can be placed in the pond culture process to achieve the purpose of reducing the total nitrogen in the culture water body. The method provided by the invention has the effect of reducing the total nitrogen in the aquaculture water body so as to purify the water quality.

Preparation method and application of recombinant engineering bacterium capable of secreting N-acyl homoserine lactone acylase

The invention provides a preparation method of a PvdQ recombinant engineering bacterium, the PvdQ recombinant engineering bacterium can secrete N-acyl homoserine lactone acylase, and the preparation method specifically comprises the following steps: S1, constructing a recombinant expression vector pET-PvdQ containing a PvdQ gene; and S2, transforming the recombinant expression vector pET-PvdQ into Escherichia coli BL21 to obtain the PvdQ recombinant engineering bacterium. The recombinant engineering bacterium prepared by the invention can efficiently secrete PvdQ acylase, and the acylase can inhibit a microbial quorum sensing (QS) system, so that bacterial biofilm pollution can be controlled, and formation of drug resistance of pathogenic bacteria is avoided.

Playing test method and device, storage medium and electronic device

The invention discloses a playing test method and device, a storage medium and an electronic device, and relates to the technical field of smart home, and the playing test method comprises the steps: calling a playing test interface of target equipment, and obtaining a target file returned by the playing test interface; determining a to-be-tested music list from the target file according to a first index, and extracting a playing link of each piece of music from the music list according to a second index; and verifying the music list and the playing link of each piece of music, and determining a playing test result according to a verification result. By adopting the technical scheme, the problem that the testing efficiency is relatively low due to the fact that music testing is carried out on the equipment in a manual mode is solved.

LuxM recombinant engineering bacterium and preparation method of acyl homoserine lactone signal molecule

The invention provides a preparation method of LuxM recombinant engineering bacteria, the LuxM recombinant engineering bacteria can secrete acyl homoserine lactone, the preparation method specifically comprises the following steps: S1, constructing an escherichia coli recombinant expression vector pET-LuxM containing a LuxM gene; and S2, transforming the recombinant expression vector pET-LuxM into escherichia coli, so as to obtain the LuxM recombinant engineering bacterium. The recombinant engineering bacteria prepared by the method can overexpress acyl homoserine lactone (AHL) synthetase, so that bacteria are promoted to synthesize more AHL signal molecules, the AHL signal molecules can be efficiently produced, a large amount of AHL signal molecules can be obtained, and the process cost is saved.

Method for increasing survival rate of out-of-season fry

The invention discloses a method for increasing the survival rate of out-of-season fry, and belongs to the technical field of aquaculture. The method comprises the step of alternately feeding a feed A and a feed B into anti-season fries, the feed A is prepared by adding berberine into a basal feed; and the feed B is prepared by adding polyhydroxybutyrate into the basal feed. According to the method, the two kinds of feed added with BBR and PHB are fed in a scientific alternate feeding strategy for breeding, the growth performance and the survival rate of the anti-season offspring seeds are improved, the intestinal tract and liver health and the body immunity of the offspring seeds are remarkably improved, no adverse effect is generated on the production performance of aquatic animals, and the survival rate of the offspring seeds is increased. The method lays an important foundation for autumn breeding and popularization of the fries.

Treatment device and process for continuously preparing sodium salt from molten bisphenol A

The invention discloses a treatment device and process for continuously preparing sodium salt from molten bisphenol A. The molten bisphenol A is conveyed to a bisphenol A sodium salt reaction tank A/B by utilizing a bisphenol A conveying pump and is uniformly distributed through a feeding spray head; caustic soda and deoxygenated water are prepared into a caustic soda solution through a mixer, and the caustic soda solution and bisphenol A are fully mixed and reacted in a first tank; overflowing to a reaction tank II, and overflowing the reacted material to a bisphenol A sodium salt reaction tank III; the materials are conveyed to a bisphenol A sodium salt cooling cooler, and unqualified materials are conveyed to a first reaction tank. According to the invention, the stability and continuity of the reaction are ensured, so that the technological process and equipment structure between bisphenol A and polycarbonate are simplified, meanwhile, unmanned operation of the device on site can be ...

Cationic lipid compound, preparation method and application thereof, and mRNA delivery system

The invention relates to a cationic lipid compound, a preparation method and application thereof, and an mRNA delivery system, and relates to the technical field of medical biology. The problem that cationic lipid with high delivery efficiency and good biological safety needs to be developed urgently is solved. According to the main technical scheme, the structure of the cationic lipid compound is shown in the specification, wherein R1 is a chain structure containing tertiary amine; r2 is a chain fatty acyl group; and R3 is a chain fatty acyl group with a branched chain. The invention mainly develops a cationic lipid compound with high delivery efficiency, organ targeted delivery and good biological safety, and the cationic lipid compound is used for preparing an mRNA delivery system and nucleic acid drugs.

System and method for recovering phenol in bisphenol A by-product

The invention provides a system and a method for recovering phenol in bisphenol A by-products, the system comprises a recovery tower and is characterized in that the bottom of the recovery tower is communicated with a tower bottom pump, the outlet of the tower bottom pump is communicated with a tower kettle reboiler, and the outlet of the tower kettle reboiler is communicated with the recovery tower; the top of the recovery tower is communicated with a tower top condenser, an outlet of the tower top condenser is communicated with a reflux tank, a first outlet of the reflux tank is communicated with a vacuum pump, a second outlet of the reflux tank is communicated with a reflux pump, and the reflux pump is communicated with the recovery tower. According to the system and the method, reduction and resource utilization of by-products in the bisphenol A raw material recovery stage can be realized.

Sargassum pallidum polyphenol extract as well as preparation method and application thereof

The invention belongs to the technical field of natural medicine preparation, and particularly relates to a sargassum pallidum polyphenol extract as well as a preparation method and application thereof. The total phenol content of the sargassum pallidum polyphenol extract is 70 g GAE/100 g to 80 g GAE/100 g, and the content of brown algae polyphenol is 10 g PE/100 g to 20 g PE/100 g. The sargassum pallidum polyphenol extract can effectively improve the symptoms of ulcerative colitis, including weight loss, diarrhea, hematochezia and the like; the traditional Chinese medicine composition can relieve inflammation symptoms and alleviate intestinal symptoms by regulating inflammatory factors TNF-alpha, IL-1beta, INF-gamma and IL-10.

Preparation process of plastic human body positioning pad

The invention discloses a preparation process of a plastic human body positioning pad, which comprises the following steps: pouring polystyrene foaming particles and an adhesive into a kneading machine according to a weight ratio of 1: (0.5-1.5), and stirring, so that the polystyrene foaming particles are uniformly wrapped by the adhesive; the preparation method comprises the following steps: adding 2-15% of PVME into a low-temperature thermoplastic material, carrying out blending granulation by using a double-screw extruder, and processing the modified granules into powder for later use in an environment of-20-40 DEG C; and putting the powder in the step S2 and the polystyrene foaming particles in the step S1 into a powder mixer according to a ratio of 1: (0.2-1), and mixing. The curing time of the material is changed by modifying the low-temperature thermoplastic material, the thermoplastic material is subjected to powder grinding by adopting a low-temperature grinding technology, the ...