30-05-2023 дата публикации
Номер: CN116178407A
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The invention discloses a near-infrared fluorescent probe for detecting peroxynitrite and a preparation method thereof, and the preparation method specifically comprises the following steps: mixing (E)-2-(3-formyl-4-hydroxystyryl)-5, 5-dimethylcyclohexyl-2-ene-1-subunit malononitrile, 4-bromomethylphenylboronic acid pinacol ester and K2CO3 in DMF (Dimethyl Formamide) in an argon atmosphere, stirring, monitoring through TCL, after the reaction is finished, washing with water, and drying to obtain the near-infrared fluorescent probe for detecting peroxynitrite. Extracting, drying and performing column chromatography separation and purification to obtain an orange solid, namely the near-infrared probe. The probe can show high selectivity and strong anti-interference capability to ONOO <->, the detection limit LOD is 97nM, and in the process of activating the probe by ONOO <->, fluorescence emission is subjected to red shift, the intensity is enhanced, and the fluorescence color is also changed ...
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