18-07-2023 дата публикации
Номер: CN116439131A
Автор:
YUAN QIN,
WANG WENBIAO,
DU MEIHOU,
LI PING,
LI XIANGRU,
LIU DONGSHENG,
LI TING,
TIAN WANG,
ZHANG JISHU,
SU JIANYING,
YE LINA,
YANG XUEQING,
XIAN XIAN
Принадлежит:
The invention discloses a tissue culture seedling raising method of sand-fixing plant Polygonum sambucifolium, which comprises the following steps: step 1, collecting explants of Polygonum sambucifolium, cutting into sections and disinfecting, step 2, carrying out induction culture on the explants by using a starting culture medium to obtain tissue culture seedlings, step 3, carrying out multiplication culture on the tissue culture seedlings by using a multiplication culture medium to obtain multiplication tissue culture seedlings, and step 4, culturing the multiplication tissue culture seedlings to obtain the sand-fixing plant Polygonum sambucifolium. Step 4, performing rooting transfer culture on the proliferation tissue culture seedlings by using two rooting culture media to obtain polygonum sambucifolium seedlings; the method is simple to operate, does not need to induce callus, directly utilizes stem segments with axillary buds to induce buds, then carries out multiplication culture ...
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