01-02-2018 дата публикации
Номер: KR1020180011473A
Принадлежит:
The present invention relates to a method for evaluating quality of hovenia dulcis extract, wherein pharmacological substances contained in hovenia dulcis extract can be clearly separated for a short period of time, and qualitative and quantitative analysis of a plurality of the isolated pharmacological substances can be made in real time. The method comprises the steps of: using a developing solvent to make a sample containing hovenia dulcis extract pass through liquid chromatography to separate ampelopsin, taxifolin, myricetin and quercetin; and qualitatively or quantitatively analyzing the separated ampelopsin, taxifolin, myricetin and quercetin. The developing solvent includes a carboxylic acid compound and a nitrile- based compound, and a volume ratio of the carboxylic acid compound solution and the nitrile-based compound solution is maintained at a ratio (R1) ranging from 90:10 to 70:30 from a start (0 min.) until 1 to 10 min. (t1) from the start; is gradually altered from the R1 ...
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